KR101731618B1 - Single nucleotide polymorphism marker composition for identification of paternity and its use - Google Patents

Abstract

A marker composition for identifying a paternity, a microarray comprising the same, and a marker composition for identifying a paternity. According to the marker composition for identifying a paternity according to one embodiment, the same paternity can be effectively confirmed.

Description

[0001] MARKER COMPOSITION FOR PATIENT DETECTION AND USE THEREOF [0002]

A marker composition for identifying a paternity, a microarray comprising the same, and a marker composition for identifying a paternity.

Since the development of DNA profiling and DNA fingerprinting for personal identification in 1985 by JEFFREIS in the UK, progress has been made, and through the standardization process over the last decade, the current STR gene marker analysis Has been established and used, and is now widely used in many countries due to its excellent analytical ability and discrimination. However, despite the high discriminatory power and excellent analytical ability, there is a possibility of misidentification due to the relatively high mutation rate (~ 10 ^-3 ), and in particular, the number of missing persons It is confirmed that there are some problems in the discrimination power.

As a part of efforts to solve these problems, it is possible to estimate physical characteristics such as race estimation, blood type, eye color as well as individual identification with a low mutation rate (2 to 2.5 X 10 ^-8 ) Research and development on SNP markers capable of system that can read out is progressing steadily.

Krawczak reported that the paternity exclusion power of an STR marker is similar to that of SNPs with an allele frequency of 0.5 (Gill et al., 2000). Gill et al. Reported that 50 SNPs with allelic frequencies between 0.2 and 0.8 Was 0.999, which is similar to 12 STR.

Since the Y chromosome is inherited only by the paternal system, the male, male ancestor, and male offspring with the normal genotype have the same Y chromosome. Therefore, it is possible to identify relatives of the same parent, such as brothers, grandchildren, grandchildren, grandchildren, and uncle-nephews through the Y chromosome gene test.

In 2003, Juan J. Sanchez et al. Found 35 SNPs, while Vallone and Butler discovered 50 SNP markers in Caucasian and African American. These results are mainly obtained from the study of Western people, and show a great difference from Korean MAF, which is a limitation to apply directly to Koreans. Therefore, selection of Y-chromosome SNP markers that are effective in Korean requires extensive screening through large-scale SNP data, and development of a single nucleotide polymorphism marker for paternity discrimination suitable for Korean population for paternity search is required .

One aspect is to provide a marker composition for paternity identification.

Another aspect is to provide a microarray comprising a marker composition for paternity identification.

Another aspect is to provide a method for identifying the same paternity using a marker composition for identifying the paternity.

In one aspect, at least one polynucleotide or a complementary polynucleotide thereof selected from the group consisting of the nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 27 has a single nucleotide polymorphism (SNP) at the 101st position of the polynucleotide, And a polynucleotide consisting of at least 10 contiguous nucleotides, including the base sequence.

According to one embodiment, the marker composition may be for identifying the same paternal relationship selected from the group consisting of between riches, between brothers, between grandparents and grandchildren, and between uncles-nephews.

The usefulness of the single base polymorphic marker composition used for identification of the paternity according to one embodiment can be judged by the "matching probability (PI)" and "power of exclusion".

The term "matching probability (PI)" means the probability that a particular genotype will be found by chance in any population. For a combination of several markers, we determine the usefulness as a marker for paternity confirmation by the cumulative probability. In order to determine the exact paternity relationship, a marker with a lower probability of being mated or a cumulative probability is required.

The term "Power of Exclusion (PE)" means the ability to exclude the possibility that a relative, especially a father, of a man is a biological father. Paternity exclusion depends on the genetics of both mother and child, and on the ethnic background of the mother and the presumed father.

The polynucleotide marker for paternity confirmation consisting of 10 or more consecutive nucleotides including the SNP located at position 101 of SEQ ID NO: 1 to SEQ ID NO: 27 has a maximum (1-2.83345E-23) at minimum 0.996694 when 27 markers are used, (Overall EP: exclusion probability) .

In the present invention, "polynucleotide" may be DNA or RNA. The polynucleotide may also be in single stranded or double stranded form. The polynucleotide may also be modified so that the sugar, base or phosphate site of a natural nucleotide, an analogue of a natural nucleotide, or a natural nucleotide is modified, as long as it has a property of hybridizing to a complementary nucleotide by hydrogen bonding, (Scheit, Nucleotide Analogs, John Wiley, New York (1980); Uhlman and Peyman, Chemical Reviews, 90: 543-584 (1990)), and nucleotides selected from the group consisting of . In addition, the polynucleotide may comprise a peptide nucleic acid (PNA). The polynucleotide can be detected, for example, in a manner such that a detectable label (for example, a fluorescent substance such as Cy3 or Cy5) is changed to a 3 'position in order to facilitate detection of the polynucleotide or a complex to which the polynucleotide is bound, Terminal or 5 ' end.

According to one embodiment, the polynucleotide exhibits a single base polymorphism at the SNP site. If one single-stranded polynucleotide is associated with the determination of a paternal relationship, it can be determined that the polynucleotide complementary to the single-stranded polynucleotide is also naturally associated with the determination of the paternal relationship. Thus, a polynucleotide according to one embodiment of the present invention may comprise a single-stranded polynucleotide and a polynucleotide with a complementary sequence thereof that are associated with a determination of a paternal relationship having one particular sequence. For example, the polynucleotide of SEQ ID NO: 1 is the 101st (SNP position) nucleotide is "C or A ". In this case, the polynucleotide includes not only 10 or more consecutive nucleotides selected from the polynucleotide of SEQ ID NO: 1 but also the nucleotide sequence of " C or A "at the 101st (SNP position) Quot; G < / RTI > or T "nucleotides at a single site. In this regard, all sequences herein are referred to by reference to sequences on the sense strand in the genomic DNA, unless otherwise noted. In the present invention, single nucleotide polymorphism (SNP) is used as is commonly known in the art. SNPs can represent a single nucleotide polymorphism present in the genome within a population. The SNP may have a frequency of a minor allele of the SNP in the population of 1% or more.

In one embodiment of the invention, the polynucleotide may be 10-100 nucleotides in length. For example, the polynucleotide may be 10 to 50 nucleotides in length, or 10 to 30 nucleotides in length.

According to one embodiment, the polynucleotide may be a primer or a probe.

The term "primer " refers to a single-stranded polynucleotide capable of acting as a starting point in the polymerization of a nucleotide by a polymerase. For example, the primer can serve as a starting point for template-directed DNA synthesis in the presence of suitable conditions, i.e., four different nucleoside triphosphates and polymerases, at the appropriate temperature and in the appropriate buffer Lt; RTI ID = 0.0 > polynucleotides < / RTI > The appropriate length of the primer may vary depending on various factors, for example, temperature and use of the primer. The primer may be 10 to 50 nucleotides in length. In general, the shorter the length of the primer, the more stable hybrid complex can be formed with the template at lower annealing temperatures.

The sequence of the primer does not need to have a sequence completely complementary to a partial sequence of the template, and it is sufficient if the primer has complementarity within a range capable of hybridizing with the template to perform the primer-specific function. Therefore, the primer includes not only the polynucleotide itself but also a sequence that specifically hybridizes to the polynucleotide, and can act as a starting point in the polymerization reaction. For example, it may be a sequence completely complementary to the polynucleotide of SEQ ID NO: 1, or a sequence complementary to such a sequence that hybridizes to this sequence and can perform a primer action. The design of the primer can be readily carried out by those skilled in the art by reference to the sequence of the target nucleic acid to be amplified. For example, it can be designed using a commercially available program for primer design. Commercially available primer design programs include, but are not limited to, the PRIMER 3 program, for example.

According to one embodiment, when the polynucleotide is used as a PCR primer, in addition to the polynucleotide, it may include a primer that specifically binds to its complementary strand.

The term "probe" refers to a polynucleotide that specifically binds to a specific target sequence. The polynucleotide may be DNA or RNA. The polynucleotide may be in single stranded form. The polynucleotide may also be a polynucleotide which is not only composed of a natural nucleotide but also a natural nucleotide, an analogue of a natural nucleotide, a sugar, a base or a phosphate site of a natural nucleotide, which is capable of being hybridized to a complementary nucleotide by hydrogen bonding And nucleotides selected from the group consisting of combinations thereof. The polynucleotide includes PNA. The polynucleotide may be a detectable label (for example, a fluorescent substance such as Cy3 or Cy5), for example, in order to detect the polynucleotide or a complex to which the polynucleotide is bound in the assay reaction, Terminal or 5 ' end.

The probe may be an entirely complementary sequence to the polynucleotide comprising the SNP site. In addition, the probe may have a substantially complementary sequence within a range that does not interfere with specific hybridization to the polynucleotide including the SNP site. In addition, the probe may have a modified nucleotide within a range that does not impair the specific hybridization to the polynucleotide including the SNP site. Examples of such probes include a perfect probe consisting of a sequence complementary to the polynucleotide including a SNP site, and a polynucleotide comprising a SNP site, And a mismatch probe having a complementary sequence.

The primers and probes can be used to amplify or confirm the presence of nucleotide sequences with specific alleles at the SNP site.

Another aspect provides a pantograph identification microarray comprising the marker composition for identifying the paternity.

The term "microarray" in the present invention means that the polynucleotide is immobilized at a high density in the divided region of the substrate surface. The microarray may be such that the region is arranged on the substrate at a density of, for example, 400 / cm ² or more, 10 ³ / cm ² , or 10 ⁴ / cm ² .

Another embodiment of the present invention provides a kit for identifying a paternity comprising the marker composition for identifying the paternity.

The polynucleotide markers in the kit may be in the form of microarrays that are arranged at high density in distinct regions on the substrate surface. In one embodiment of the present invention, the polynucleotide marker comprises at least one polynucleotide selected from the group consisting of the nucleotide sequences of SEQ ID NOS: 1 to 27, or a complementary polynucleotide thereof, wherein the polynucleotide has a single base Polymorphism (SNP) exists and may be a combination of polynucleotides consisting of 10 or more consecutive nucleotides, including the 101st nucleotide.

The kit may be a kit for specifically amplifying the polynucleotide and confirming the same paternity through the presence or absence of an amplification product. In this case, the kit may include the polynucleotide as a primer and the reagent necessary for amplification. The amplification reagent may comprise, for example, dNTPs, polymerases, and suitable buffers. For example, the primer may be one in which the nucleotide sequence corresponding to the SNP site forms the 3'-terminal nucleotide of the primer, the 3'-terminal nucleotide is complementary to the nucleotide at the SNP site (specific primer) (Non-specific primers). The non-specific primer may include a sequence that is not complementary to the 3 'terminal nucleotide as well as other sites. The kit may also include instructions for use. For example, in the case where the target sequence is amplified in the amplification reaction using the specific primer and the target sequence is not amplified in the amplification reaction using the non-specific primer, May include an explanation of the result determination, including determining that the sequence is present and identifying individuals from the results.

The kit may be a kit for hybridizing the polynucleotide or a probe derived therefrom with a nucleic acid in a sample and identifying an individual from the hybridization result. In this case, the kit may include the probe and a reagent necessary for hybridization. Reagents necessary for hybridization include, for example, a hybridization buffer. The nucleic acid may not be amplified or amplified. Thus, the kit may further comprise reagents necessary for amplification of the nucleic acid. The nucleic acid may be labeled with a detectable label. The kit may comprise a perferct match probe that is completely complementary to the polynucleotide or a mismatch probe that is complementary at all sites except for the SNP site in the polynucleotide. The probes may be in the form of microarrays fixed to a plurality of discrete regions on the substrate. The kit may also include instructions for use. For example, when the target sequence is detected in the hybridization reaction using the fully complementary probe and the target sequence is not detected in the hybridization reaction using the mismatch probe, the user's manual may include a profile obtained by each probe And a description of the result determination, including an explanation of determining that the same sub-category is determined from the result.

According to one embodiment, the kit may be a kit for identifying the same parent-child relationship selected from the group consisting of between rich, brothers, grand-grandchildren, and uncle-nephews.

Yet another aspect provides a method for detecting nucleic acid, comprising: providing an isolated nucleic acid sample; And

And determining the nucleotide at the SNP position of the polynucleotide contained in the marker composition for identifying the pendant.

A method for identifying the same paternity according to one embodiment may comprise providing a separate nucleic acid sample. Methods for separating nucleic acids from an individual are known in the art. For example, DNA can be isolated directly from tissues or cells by amplifying specific regions by nucleic acid amplification methods such as PCR. The separated nucleic acid sample includes not only the purely isolated nucleic acid but also a cell lysate containing a crude nucleic acid, for example, a nucleic acid. The nucleic acid amplification method may include PCR, ligase chain reaction (LCR), transcription amplification, self-sustained sequence replication, and nucleic acid-based sequence amplification (NASBA). The separated nucleic acid may be DNA or RNA. The DNA may be a genomic DNA, a cDNA, or a recombinant DNA. The RNA may be mRNA.

The method may further comprise determining the nucleotide at the SNP position of the polynucleotide contained in the marker composition for identifying the pendant.

According to one embodiment, the step of determining the nucleotide at the SNP position of the polynucleotide marker can be performed by sequencing, hybridization with a probe, or single base primer extension.

Methods for determining the nucleotides at SNP positions are well known in the art. For example, the nucleotide at the SNP position can be determined directly by a nucleotide sequencing method of known nucleic acids. The nucleotide sequence determination method may include a sanguine (or dideoxy) method or a curd-gilbert (chemical cleavage) method. In addition, the nucleotide at the SNP position can be determined by hybridizing a probe containing the sequence of the SNP position with the polynucleotide of interest, and analyzing the hybridization result. The degree of hybridization can be confirmed, for example, by marking a detectable label on the target nucleic acid, detecting the hybridized target nucleic acid, or confirming it by an electrical method or the like. In addition, a single base primer extension (SBE) method can be used.

In the above method, the step of determining the nucleotide at the SNP site comprises: hybridizing the nucleic acid sample to a microarray in which the polynucleotide marker is immobilized; And detecting the hybridization result.

According to one embodiment, the method for identifying the same paternity may further comprise comparing the nucleotide at the SNP position of the polynucleotide included in the determined paternity identifying marker composition to the result of the control sample. According to one embodiment, the control sample may be a DNA of an individual to be compared or a DNA profile of a registered individual.

According to the marker composition for identifying a paternity according to one embodiment, the same paternity can be effectively confirmed.

Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments, and the scope of the invention is not limited to these embodiments.

Example  1: for checking paternity SNP Marker  selection

(1) Subject classification

Genome-wide genome-wide human SNP array 6.0 of Affymetrix was applied to 6586 Korean specimens to determine the minimum level of call rate per sample (the ratio of genotype results to total SNPs) to SNPs of more than 90% .

(2) SNP Marker  selection

In Affymetrix Genome-Wide Human SNP Array 6.0, there are 257 SNPs located on the Y chromosome. Of these, 6586 Korean males selected SNP with a call rate of 90% or more and MAF of 10% or more. In order to select the Y chromosome-related monoclonal polymorphism markers for identifying the correct paternal markers, markers were selected after statistical processing in the following manner.

The conservative method r-square was applied to the estimation of LD. If the LD is high among the SNPs, the SNP information is duplicated. In this case, the tagging SNPs can be selected and sufficient information can be obtained with a small number of SNPs. In this embodiment, the tagging SNP is selected based on the strong LD with r-square of 0.8 or more.

A total of 27 SNPs were selected from the Y chromosome based on the above criteria, and a list of selected SNPs is shown in Table 1 below

number rs_number chromosome position allele_A allele_B Freq_A Freq_B AA_
freq AB_
freq BB_
freq One rs11096432 Y 20064289 C T 0.77 0.23 0.77 - 0.23 2 rs13305612 Y 9191385 C T 0.11 0.89 0.11 - 0.89 3 rs16980363 Y 14712374 G C 0.8 0.2 0.8 - 0.2 4 rs16980601 Y 13924509 A G 0.23 0.77 0.23 - 0.77 5 rs17249791 Y 17269063 C T 0.12 0.88 0.12 - 0.88 6 rs17250114 Y 19294091 C G 0.92 0.08 0.92 - 0.08 7 rs17250121 Y 19296941 C T 0.18 0.82 0.18 - 0.82 8 rs17269396 Y 12798981 A G 0.12 0.88 0.12 - 0.88 9 rs17269816 Y 15563165 C T 0.61 0.39 0.61 - 0.39 10 rs17269928 Y 17156436 A G 0.63 0.37 0.63 - 0.37 11 rs17276358 Y 17688857 T G 0.38 0.62 0.38 - 0.62 12 rs17276777 Y 6010884 G A 0.89 0.11 0.89 - 0.11 13 rs17315694 Y 17295568 G A 0.91 0.09 0.91 - 0.09 14 rs17316007 Y 12511232 G A 0.2 0.8 0.2 - 0.8 15 rs17323322 Y 21156174 T C 0.88 0.12 0.88 - 0.12 16 rs1846574 Y 8081340 C T 0.2 0.8 0.2 - 0.8 17 rs2032595 Y 13323385 T C 0.99 0.01 0.99 - 0.01 18 rs2032652 Y 20376701 T C 0.82 0.18 0.82 - 0.18 19 rs2032665 Y 14036145 C T 0.82 0.18 0.82 - 0.18 20 rs2075640 Y 2782506 G A 0.18 0.82 0.18 - 0.82 21 rs2191902 Y 16272062 T C 0.18 0.82 0.18 - 0.82 22 rs3853054 Y 8739843 T G 0.82 0.18 0.82 - 0.18 23 rs3863116 Y 15463504 A T 0.23 0.77 0.23 - 0.77 24 rs4121530 Y 6721973 A C 0.84 0.16 0.84 - 0.16 25 rs4589047 Y 14751710 A T 0.18 0.82 0.18 - 0.82 26 rs7067226 Y 20443215 C T 0.99 0.01 0.99 - 0.01 27 rs891234 Y 14810699 T C 0.64 0.36 0.64 - 0.36

In Table 1, the numbers indicate the sequence numbers on the sequence listing, and rs_number means the reference sequence. After the human genome project, the single nucleotide sequence (SEQ ID NO: 1) in the database of the National Institute of Biotechnology Information (NCBI) This is the name given to distinguish the base polymorphism. The chromosome represents the number of the chromosome in which the single nucleotide polymorphism is located. Position indicates the position where each sequence number exists on each chromosome.

Allele_A and allele_B are randomly named alleles for ease of experiment in genotyping, and Freq_A and Freq_B represent frequencies of alleles A and B, respectively. AA, AB and BB are the number of subjects with a genotype, and AA_freq, AB_freq and BB_freq represent the frequency.

(3) The same paternity criteria

When the genotypes of the samples to be compared are clearly different from each other among all the markers, it is a rule to judge that the same paternity relationship is not obtained when one item is different from another. In this case, the paternity identification index is set to 0, If all of the items match, a genotypic match is determined and a probabilistic estimate of the same parent is provided.

If we assume that allele a _i is in the locus Y of the putative Y chromosome, the putative son also has allele a _i . In this case, the Exclusion probability (EP) is (1-p _i ). Where p _i is the allele frequency of a _i (frqeuency). The exclusion probability from Locus l is EP _l , The overall exclusion probability of combining K SNP information is as follows.

Overall EP =

Since the Y chromosome, unlike autosomes, has only one allele in one locus, both the estimated father and the presumed son, the overall exclusion probability is briefly summarized as follows.

Overall EP =

Where p _{i l} is the frequency of allele a _i in locus l (frqeuency).

SNP markers have limited coverage to family relationships between identical parental families in which the common allele is necessarily present in the Mendelian genetic code. The criterion was applied to 99.9% of the AABB guidelines for the 1: 1 designation comparison case, which requires confirmation of the family relationship by prior information, as the first criterion for the third person survey. In addition, more than 99.99% of the cases of missing persons, finding missing persons, mass casualties, etc.

Example  2: Example of identification of the same parent and results

The results are shown in Table 2 below. [Table 2] < tb > < TABLE >

number rs_number Rich Relationship 1 Genotype Rich relationship 2 genotype Rich relationship 3 genotype Part 1 1 Exclusion rate Part 2 2 Exclusion rate Part 3 Now 3 Exclusion rate One rs11096432 C C 0.77 T T 0.23 C C 0.77 2 rs13305612 T T 0.89 T T 0.89 C C 0.11 3 rs16980363 C C 0.20 G G 0.80 G G 0.80 4 rs16980601 G G 0.77 A A 0.23 G G 0.77 5 rs17249791 T T 0.88 T T 0.88 T T 0.88 6 rs17250114 C C 0.92 C C 0.92 C C 0.92 7 rs17250121 C C 0.18 T T 0.82 C C 0.18 8 rs17269396 G G 0.88 A A 0.12 G G 0.88 9 rs17269816 C C 0.61 C C 0.61 C C 0.61 10 rs17269928 A A 0.63 A A 0.63 G G 0.37 11 rs17276358 T T 0.38 G G 0.62 G G 0.62 12 rs17276777 G G 0.89 A A 0.11 G G 0.89 13 rs17315694 A A 0.09 G G 0.91 G G 0.91 14 rs17316007 A A 0.80 A A 0.80 A A 0.80 15 rs17323322 T T 0.88 T T 0.88 C C 0.12 16 rs1846574 C C 0.20 T T 0.80 T T 0.80 17 rs2032595 T T 0.99 T T 0.99 T T 0.99 18 rs2032652 T T 0.82 T T 0.82 T T 0.82 19 rs2032665 C C 0.82 C C 0.82 C C 0.82 20 rs2075640 A A 0.82 A A 0.82 A A 0.82 21 rs2191902 C C 0.82 C C 0.82 C C 0.82 22 rs3853054 T T 0.82 T T 0.82 T T 0.82 23 rs3863116 T T 0.77 A A 0.23 T T 0.77 24 rs4121530 A A 0.84 A A 0.84 C C 0.16 25 rs4589047 T T 0.82 T T 0.82 T T 0.82 26 rs7067226 C C 0.99 C C 0.99 C C 0.99 27 rs891234 C C 0.36 T T 0.64 T T 0.64 Overall EP 99.9998454% 99.9998515% 99.9998632%

As a result of analysis of each pair of rich relationships, all negative alleles in the family were observed, and in this case, it was confirmed that the exclusion power for paternity was at least 99.9998454% (the possibility of the same paternity can not be excluded) There were many incongruous alleles among the rich of other families, and it was confirmed that it was not a rich relationship.

&Lt; 110 > DNA LINK, INC. <120> Single nucleotide polymorphism marker composition for          identification of paternity and its use <130> PN096234 <160> 27 <170> Kopatentin 1.71 <210> 1 <211> 201 <212> DNA <213> Homo sapiens <400> 1 tcctttgaat tgaataatga taatgacaca agttaccaaa aagtgtggga tacagtgaaa 60 tcagtgcttt acaaaaaaag gttatagcac tgaatgtcta cgtagaaaag cctgaagggg 120 tcaagggcag aaactgacaa cctcctgtca tacctcaatg aactagagaa acaagaacaa 180 actaaaccca aagctagaag a 201 <210> 2 <211> 201 <212> DNA <213> Homo sapiens <400> 2 agcactgtcc atgtcctctg tcttcacctt ttttgcaggt gaagttgcag gaccccgtcc 60 acccctcacc agattgtatg ctcaccccta tgtgaactta cggctgctca cactctctgt 120 ttcagaatga aatcccaaga caatggagga gtttccccta atgacgttaa gcacctgctt 180 ggctgggaaa tgaattggag g 201 <210> 3 <211> 201 <212> DNA <213> Homo sapiens <400> 3 tggtccttag gctcctattt cctcatggct tagacttttt atatggaaat gcagctctgt 60 cactgtcata gcacacagtg gtagctggcc ttgccaccag ctagatttgg cttgatggaa 120 acctgaatat caatttctag ttattatgaa atagacactc ttattcttca gtgactttta 180 agaatttttt ttgaattatc t 201 <210> 4 <211> 201 <212> DNA <213> Homo sapiens <400> 4 tagctatagt cattagtagt tccttctcta taaacgactt ctcaattctc gcaaatagta 60 gcagaaacct tattaagaaa ggtatagtgt tcaaaatgta acctgtctcc taccactctt 120 ggctcctacc acatgtcaaa tgttagcata taatgtaaag aatcataact cttccacaat 180 gagtaggtgg atggcactgc c 201 <210> 5 <211> 201 <212> DNA <213> Homo sapiens <400> 5 ccttttatca agccatcctc aattatcctt acatgagagt gccatctgtt tcccttaaac 60 ctcaactcag acaagtagag ctaaatatat taatgtctta cgaatccatc ccaaaaattc 120 tcggtgaaat ttcaagcaaa tacaaatgcc catgttcatt ttccacattc cacacaagac 180 atgtcgtata catttattct g 201 <210> 6 <211> 201 <212> DNA <213> Homo sapiens <400> 6 gaatttttgt ttgtttttta ttcctttgct cattttcccc ttccaacaat ggtctccttt 60 ttgcccacca cccaaatcct acccaaggcc ttgatggagt cacatatatt ccaagagatg 120 atctagatca cttccatctt ccaccacctc ccctttacac tccttactta gtttaccatg 180 ttattttaat tcctgctcaa t 201 <210> 7 <211> 201 <212> DNA <213> Homo sapiens <400> 7 gaaagggtaa agtattttct tatctttttt tggattattt attaagagtt tcttcctcca 60 ctgactgtaa aatgctgaat tatagttaag tgtttataga ctaggtctgt ttctccctct 120 ttgaattcat attgtacctg cactaaatat atttgttata taccaagtgg gtactaaatt 180 tgaaaaaaaa aatttggctt c 201 <210> 8 <211> 201 <212> DNA <213> Homo sapiens <400> 8 tgatttcagc tccatgaaaa actgttccct aaaacaaggg gtcgattgat taaaataacg 60 aaagaaaaga aaatcagaat aataacagag taattggcag acgtatacaa tagacttttt 120 ttctttcttt tatatatata tttttttatt atactttagg ttcaagggca catgtgcaca 180 acgtgcaggt ttgttacata g 201 <210> 9 <211> 201 <212> DNA <213> Homo sapiens <400> 9 ccttttta gccaaaaaat tgagtagcac tttaaaacaa gacagagaga ctcactttgc tgtgtttaga 120 attgattttc tcctggaaaa tgtagactgg tgttacatgc catatttaga agttagccac 180 ttatgctctc ataattttat t 201 <210> 10 <211> 201 <212> DNA <213> Homo sapiens <400> 10 agaaaagatt gctctaatca gcctatttat ttaaaattgt ttaagatagg taagtgttcc 60 tagcttagca gttattgcaa tcagaaatgg gaagacttca agaatttcta tgttgctctt 120 cggaagtgta aagtcattta gctgtatgat cctttattgc catttttttt ctgacagatt 180 ttatgaattt atctttgtct c 201 <210> 11 <211> 201 <212> DNA <213> Homo sapiens <400> 11 ccaaccaatg cttaaataag tggggaaaac attgctttca cctctgaggc cagcgaaatg 60 atgactaatt ggttgcacaa caatctgtta cagaccttct gcttgaaagg ttattaccct 120 tctgctgtcc ttcagcagaa acatgattag ttttcaaacc cttgtttatt taactcaatc 180 atcctatgta gctttctcta g 201 <210> 12 <211> 201 <212> DNA <213> Homo sapiens <400> 12 acacaactat tttaatacta agttctgtac tcttgacatt gttttatgtt ctcttctgct 60 gatatgactg aacaaactca tctaaacatt gacaaacagc attataggac tcattaaata 120 aattctagta aatacaatag ggttctaatt tatttttgaa aggaatgagg gagacctata 180 tttactgaca tagaaagact t 201 <210> 13 <211> 201 <212> DNA <213> Homo sapiens <400> 13 ttaaatttta tttgctgatg ctgaaatgat tgaaataaca tgattaatgt tttcttgttc 60 agtttgtttt acaaggtaat aggcattgat tgggcattgt atattccaca attttacgaa 120 catttttcac tttcacataa ctgatttttt tattttgaga cagagtcagg ctttgttacc 180 taggctggat tgcagtgatg t 201 <210> 14 <211> 201 <212> DNA <213> Homo sapiens <400> 14 atgattaaaa agatattgtt tgatggttca gcttccagat attaccagca tgcagattta 60 gagaccggt gttttcttta gcattttggt cccatctttt ataagaggca gagtttccaa 120 agagggataa agaggatgaa tgggaggaaa aagaggggag catgaataat tgagaaaaaa 180 gctaaagctg acagaaagga g 201 <210> 15 <211> 201 <212> DNA <213> Homo sapiens <400> 15 aatattttct tgcaggcttg ggcaagtact gtagaccatc tgtcctcatc catttaaagg 60 ccaatggtgt ttcaggcatt cagctaggta tttcagacat cgtagttccc aaatgccggt 120 ctgttaaata gtattggtgc aggctgaatt ttcagtgctc tgaagtcaaa ttagaagata 180 catagttacg atgtttttca t 201 <210> 16 <211> 201 <212> DNA <213> Homo sapiens <400> 16 ggagttagat ctctgaaatc ctgaatggaa gagagagcac tccccttcta tcaccagaca 60 tagtatgctt gcttcacagg cttgttctag caacaaatcc cacctgctat tctccctgga 120 cagattgttt ggtaaagtat aaatgtgacg gtgaacactc ctaggccttc ttgctttctt 180 ctctgccttg ccacagcaag a 201 <210> 17 <211> 201 <212> DNA <213> Homo sapiens <400> 17 ccttttaaaa gaaaggattc atgatgaaat ctgctttttg ttttgcagag agcttggaga 60 taattctggt ggctgtgtgg agtatgtgtt ggaggtgagt cgctagctga agaattaaaa 120 caatagtttt agcagtttgg gtaagagatg tttacagaaa tgttttgtgg aataaaactg 180 aacagtcaga gacctatgag a 201 <210> 18 <211> 201 <212> DNA <213> Homo sapiens <400> 18 gagtcttatc ctatgaggtg ccatgaaagt ggggcccaca gaaggatgct gctcagcttc 60 ctggattcag ctctcttcct aaggttatgt acaaaaatct catctctcac tttgcctgag 120 ttgcagctac ctttgctggt gatcctggac ccaaagtgtg ccagcctctc ctgatactct 180 gtgtgtacct gagcagctat t 201 <210> 19 <211> 201 <212> DNA <213> Homo sapiens <400> 19 aggccatata aaaacgcagc attctgttaa tataaaacac aaaacaacct ttataacaga 60 ttttatatct attactatta catatattaa taagaagtca cgtaacgaga tgttttaagt 120 tctgaatatt ttaccatata ttacaatatt cttctctact ttttctcaag ttctctccat 180 tttgaaaatt ggaatcaatt t 201 <210> 20 <211> 201 <212> DNA <213> Homo sapiens <400> 20 caccagaggg ttctgaaaca gattccccct tggcaaggtt tcccaaatgt ctaaattact 60 aaatcagtat gggagatcat cagctctgtg actggttaat atagtttttc ccaagagcac 120 tccttctctg accgaggaaa tgctggaaat cgatgggata agtaatgtct gggtgactgc 180 tttaggctgg ctttgtgttt a 201 <210> 21 <211> 201 <212> DNA <213> Homo sapiens <400> 21 tttggctctc cctggtccta ttgctcatgg attctctctc ttctttctat tggtctcttg 60 ttttgggcat cccattttgc agtcctccaa tatggagtaa cttctctctc catccaaaag 120 aaaggtaagc taaacaatat ctccagctgt cttctaggaa ggaactactg gctctcacat 180 cagatgagta taggatttac a 201 <210> 22 <211> 201 <212> DNA <213> Homo sapiens <400> 22 tatggcttct cctgctacaa catgaaaaca tcattacaac tcataggcaa tatgaataaa 60 tactaaaggc tgcaatgaaa gattataagg gaacctgatt gcttgggagt gataattcag 120 gagaaacttt tctaaggaac tgatttaaat tatttttagt taatttgata ctgcagcttt 180 cttctgaccc tatcacattg a 201 <210> 23 <211> 201 <212> DNA <213> Homo sapiens <400> 23 tggcctattt ctccctgtca ggttgcacag atgcatgtag agcattctta ggagaccatt 60 gttttagaaa actttgattt gtacatgtta gttttcatga aattgcaaca cagagatagg 120 tcctaaaagt ggaatgtatt taaaacttgt tgaattagac acacacacac agacacacac 180 aaagaatcag cagagaaaac a 201 <210> 24 <211> 201 <212> DNA <213> Homo sapiens <400> 24 tttgaggcta caagttagtt aatcttttgg aacatgttat cttctgctat ttatagcaaa 60 gatcctaagt gtaactattg tcattttctt ctatatctgc aagttggtat gtttacatac 120 aggtgaaaga gcctcttttc catatagtct agtcataagt aaaagacctt ttaggcttag 180 gtctggaaaa cttttaagtc c 201 <210> 25 <211> 201 <212> DNA <213> Homo sapiens <400> 25 aactgctagg tcgttactag agtgcaggtc tttcctcact caaacacaaa ggcaggccag 60 cagcatcaca ctgaccaact tgagagggag aatagacaga aatatccctg cttttatact 120 caggtcccag aaaagtctat tctttcactg ggaaaaatta aaatggatgt ttgttttctg 180 tggatgcttt tttttttttt t 201 <210> 26 <211> 201 <212> DNA <213> Homo sapiens <400> 26 gatcagtgtc actgtaaagc aactatacaa acaacaaaca taataactcc atttatgtaa 60 ctatgtttac aagttaacag cacaatgtca agatcagata cacatattaa tactaatctt 120 acatgtaaat gacctaaatg tcccatttaa aaggcacaga gaagagtggc aagctggata 180 aaattgcaag actcaattgt a 201 <210> 27 <211> 201 <212> DNA <213> Homo sapiens <400> 27 ttccagggct tgagaaacca acagattgat ctgactctgt cttcatcagt ttaaaggaaa 60 gggccagtgg gataaatatg attttttttt tggtaatgat cttgaaacaa gaaagaaaaa 120 aaaaagcaat ctatttattc ttctctatat taaggtatgg tcaattggta ccaaaagaaa 180 gccacttccc tttgacttta t 201

Claims (8)

A polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 1 to SEQ ID NO: 27, or a complementary polynucleotide thereof, wherein a single base polymorphism (SNP) is present at the 101st position of the polynucleotide and the polynucleotide comprising the single nucleotide polymorphism A marker composition for identifying a paternity comprising a polynucleotide consisting of 10 or more contiguous nucleotides. 2. The marker composition according to claim 1, wherein the marker composition is for confirming the same parental relationship selected from the group consisting of between rich and poor, between brother and sister, between grandparent and grandchild, and between uncle and niece. A microarray for identifying a paternity comprising the marker composition of claim 1 or 2. Providing an isolated nucleic acid sample; And
Determining the nucleotide at the SNP position of the polynucleotide included in the marker composition of claim 1 or 2. &lt; Desc / Clms Page number 24 &gt; 5. The method of claim 4, wherein the method is for identifying a paternal relationship of the same paternity selected from the group consisting of between riches, between brothers, between grandparents, and between uncles and nephews. 5. The method of claim 4, wherein the step of determining the nucleotide at the SNP position of the polynucleotide is performed by sequencing, hybridization with a probe, or single base primer extension. 5. The method of claim 4, wherein the method further comprises comparing a nucleotide at a SNP position of a polynucleotide included in the determined paternity identifying marker composition to a result of a control sample. 8. The method of claim 7, wherein the control sample is a DNA of an individual to be compared or a DNA profile of a registered individual.