KR20100137162A - A mixer and centrifuge having temperature controlling function - Google Patents

Abstract

PURPOSE: A centrifuge with a mixer is provided to easily control temperature and to maintain a sample by having a temperature control unit. CONSTITUTION: A centrifuge with a mixer, which is able to control temperature comprises: a cover(1) of plate form, which is made of semi-transparent material; a centrifuge unit(2) having a plurality of bucket frames; a motor unit for rotating the centrifuge unit by being mounted at the lower portion of the centrifuge unit; a buffer unit for reducing noise and vibration; control panel(5) having a built-in microprocessor which controls the motor unit; and an inner case(6b) having a plurality of discharge parts(6a).

Description

A mixer and centrifuge having temperature controlling function

The present invention relates to a centrifuge, and more particularly, having a temperature control means, while maintaining a sample at a required temperature, by attaching a mixer to the centrifuge and simply and easily mixing the objects to be centrifuged continuously. The present invention relates to a mixer-use centrifuge having a temperature control function capable of increasing the efficiency of centrifugation as well as convenience of centrifugation by centrifugal separation in a short time.

In general, centrifuges are used to separate solid particles in a liquid by centrifugation that separates, purifies, and concentrates substances with different components or specific gravity by the action of centrifugal force. Also used to separate them.

These centrifuges have been developed in various types according to the target material to be centrifuged, and are widely used in chemistry laboratories.

Among them, the patent registered "0358953" as "platelet concentration centrifuge" which performs centrifugal separation by rotating the rotating drum containing a plurality of test tubes containing the material to be centrifuged at high speed by a driving means. And published in a registered patent publication.

The patent registration "0358953" "platelet enrichment centrifuge" is translucent and has a hole formed in the upper portion, and a hole is formed in the center of the conical form a plurality of holes (Hole) is formed so that the falcon tube is inserted from the inside to the outside A centrifugation means unit; A rotating member including a shock absorbing member for buffering the centrifuge to rotate and vibrate; A control unit configured to be controlled by the microcomputer according to the operation of the first and second button switches so that the rotational speed of the rotating member rotates or stops at a constant speed for a predetermined time; In order to obtain the soft layer (white blood cell layer and platelet) through two steps in blood, it consists of an inner case in which a plurality of outlets are formed so as to discharge the air generated by the centrifugal separation unit to the outside, and a body part formed in the front panel of the control panel. As a dedicated centrifuge, it is processed for anyone to use and it is convenient to operate. Especially, it has a special advantage that there is little noise and vibration at high speed.

However, as well as the "platelet concentration centrifuge" as well as various conventional centrifuges are not equipped with any function other than the centrifugal separation is used only for centrifugation.

In spite of this situation, the object to be centrifuged (hereinafter referred to as "sample") may not only need to be kept at a constant temperature from time to time, but also needs to be mixed with the sample before centrifugation. Was using a separate temperature control device or a separate mixer.

For example, when centrifuging a sample in a falcon tube with the "platelet concentration centrifuge", the sample to be centrifuged in the falcon tube is mixed by shaking the falcon tube by hand as a preliminary operation of the centrifugation operation. After the falcon tube is inserted into the hole (Hole) of the centrifugation means to increase the centrifugation efficiency by centrifugation by operating the platelet concentration centrifuge.

Therefore, mixing the target material to be centrifuged in the falcon tube by manually shaking the falcon tube is not only a very cumbersome task, but also a centrifugal separation in a short time due to the time required for mixing. There was a flaw that I could not.

Of course, using a mixer separate from the "platelet concentration centrifuge" may be mixed before centrifuging the target material to be centrifuged in the falcon tube, in this case, a separate mixer from the "platelet concentration centrifuge" Therefore, not only the space limitation to put the mixer, but also the disadvantage of using the platelet concentration centrifuge and the need to operate the mixer double, especially the cost of the separate purchase of the mixer economical There was a problem falling.

As such, the conventional centrifuge has only a centrifugal separation function, and thus, it is necessary to separately prepare a defect and a temperature control device or a mixer, which make it difficult or difficult to store the sample at a constant temperature or to mix the sample. In addition to the space constraints to be placed, there are disadvantages in that the operation of the centrifuge, the thermostat, and the mixer must be operated separately. There was a problem.

In view of the above circumstances, the present invention has been made to solve various drawbacks and problems caused by a centrifuge which centrifuges a sample to be centrifuged, especially in a plurality of Falcon tubes. It is an object of the present invention to provide a mixer and centrifuge having a temperature control function capable of facilitating the temperature control and storage of the sample by keeping the sample at the required temperature with the control means.

Another object of the present invention is to equip the centrifuge with a mixer to easily and easily mix the sample to be centrifuged and then continuously centrifuged to increase the efficiency of centrifugation as well as the convenience of use, which allows a large amount of centrifuge in a short time. It is to provide a centrifuge having a.

Another object of the present invention is not to be provided with a temperature control device or a mixer separately from the centrifuge, so it is not limited to the space to put a separate temperature control device or a mixer, as well as to purchase a separate temperature control device or mixer. It is possible to reduce the cost, and to provide a centrifuge having an excellent economic mixer.

Mixer combined centrifuge having a temperature control function of the present invention for achieving the above object is a centrifugal separator that is provided with a plate-shaped lid portion (1) made of a semi-transparent body, and a plurality of incisions in which the discs are cut at regular intervals. Means 2, a motor means 3 installed below the centrifugal means 2 to rotate the centrifugal means 2, and a cushioning means for reducing noise and vibration of the motor means 3 ( 4), a control panel 5 incorporating a microprocessor controlling the motor means 3, and a plurality of discharge ports 6a are formed to discharge the air generated by the centrifugal separation means 2 to the outside. In the centrifuge consisting of a main body 6 in which the inner case 6b is built-in and the control panel 5 is installed in the front, a mixer built up and down in the front corner of the main body 6 ( 10); Characterized in that it further comprises a temperature control means 20 is connected to one side of the body portion (6).

The present invention is provided with a temperature control means integrally with the centrifuge to keep the sample at the required temperature and to facilitate the temperature control and storage of the sample, equipped with a mixer in the centrifuge to the sample to be centrifuged Simple and easy mixing, continuous centrifugation, not only convenience in use, but also high efficiency of centrifugal separation enables a large amount of centrifugation in a short time, and there is no need to provide a temperature controller or mixer separately from the centrifuge. Not only is it not restricted by the space to place a thermostat or a mixer, but also can reduce the cost of purchasing a separate thermostat or a mixer has an excellent economical advantages.

Hereinafter, with reference to the accompanying drawings will be described in detail a preferred embodiment of the mixer combined centrifuge having a temperature control function of the present invention.

Figure 1 is a perspective view of the mixer combined centrifuge with a temperature control function of the present invention, Figure 2 is a perspective view of the lid open state of the mixer combined centrifuge with a temperature control function of the present invention showing another embodiment of the centrifuge, Figure 3 4 is an exploded perspective view of the heating means and the heating block according to the present invention, FIG. 4 is an exploded perspective view of the cooling means and the cooling block according to the present invention, FIG. 5 is a sectional view taken along the line A-A of FIG. 6B is a cross-sectional view taken along line C-C of FIG. 1, FIG. 7 is a perspective view showing various structures of a bucket insert employed in the present invention, and FIG. 8 is a state diagram of a heating block and a cooling block according to the present invention. The mixer-use centrifuge having a temperature control function has a plate-shaped lid part 1 made of a translucent body, a centrifugal separation means 2 having a plurality of bucket frames 2b having a 'Y' shape at the center and a similar shape; The centrifuge A motor means (3) installed in the lower part of the means (2) for rotating the centrifugal separation means (2), a buffer means (4) for reducing noise and vibration of the motor means (3), and the motor means (3) Control panel (5) with a built-in microprocessor controlling the control panel and an inner case (6b) having a plurality of discharge ports (6a) formed to discharge the air generated by the centrifugal separation means (2) to the outside A centrifugal separator comprising a main body portion (6) provided with a control panel (5) in the main body (6), the mixer (10) mounted up and down in the front corner portion of the main body portion (6); It is further provided with a temperature control means 20 is connected to one side of the).

The centrifugal separation means (2) is a central portion is fixed to the shaft of the motor means (3) a plurality of bucket frame (2b) of the 'Y' shape is formed at the center similarly, the end of the bucket frame (2b) A downwardly inclined groove (2c) is formed to rotate the projection (2c) in the inclined groove (2c) with the upper portion of the bucket (2a) is fixed to the bucket insert (2b ') formed on the outer periphery rotation projection (2a') 2a ') is inserted into the centrifuge having a mixer.

Here, the centrifugal separation means 2 may be configured as an angle rotor 2d in which a plurality of bucket insertion grooves 2d 'are formed downward along the edge of the cylindrical body instead of the plurality of bucket frames 2b having a' Y 'shape. have.

The mixer 10 consists of a motor 11 rotating in an eccentric shaft, an eccentric shaft 12 of the motor 11, and a funnel-shaped vibration cup 13 fitted to and fixed to the eccentric shaft 12. Switched to the downward pressing of the cup 13, the motor 11 operates and the vibration cup 13 is configured to vibrate by the eccentric rotation of the eccentric shaft 12 according to the operation of the motor 11.

The temperature control means 20 is the body portion 21 of the hexahedral shape, and the first heating means 22 and the second mounted on the rear portion of the body portion 21 so as to have an opening on the upper surface of the body portion 21 Heating means 22 ', cooling means 23 mounted at the center of the first half of the body portion 21 to have an opening on the body portion 21, and the first and second heating means 22, 22'. A cover body 24 having a front surface surrounding the upper surface of the upper surface of the mounted body portion 21; A lid mounting stand 25 in which a rear plate which stands on the rear end of the upper surface of the body portion 21 and a side plate which is installed on both sides of the rear half portion are integrally formed, and an upper end of the rear plate forms a lid mount; A lid 26 installed on the lid mounting stand 25 by a hinge 26a; A heating block 27 inserted into the first and second heating means 22 and 22 '; A cooling block 27 'inserted into the cooling means 23; The first and second heating means 22, 22 'and the control panel (28, 28', 28 ") that incorporates a microprocessor for controlling the temperature of the cooling means (23).

In addition, each of the first and second heating means 22 and 22 'includes a square pipe type heat conducting tube 22b having an upper and lower end portions extending in the right, left, right, and right directions to form a coupling portion 22a, and the heat conducting tube 22b. A heat dissipating member 22c surrounding an outer surface of the heat dissipation member, a temperature sensor 22d installed in the heat dissipating member 22c, a silver foil 22e surrounding the heat dissipating member 22c, and the heat conductive tube body 22b. The heat conduction plates 22f and 22f 'coupled to the coupling portion 22a of the lower front end portion of the bottom surface) and the plate heater 22g inserted and fixed between the heat conduction plates 22f and 22f'.

Here, the temperature control range of the first heating means 22 is 80 ° C or more and 95 ° C, and the temperature control range of the second heating means 22 'is set to 65 ° C or more and less than 80 ° C, optionally. It is preferable to be able to use, and as the heat dissipation material 22c, any one of urethane foam, styrofoam, and iso pink can be preferably used.

The heating block 27 is a block of a rectangular column formed with a plurality of cylindrical holes (27a) to be inserted into the heat conduction tube (22b).

The cooling means 23 has a rectangular pipe-type heat conduction tube 23b having an upper and lower end portions extending in the right, left, right, and right directions to form a coupling portion 23a, and a heat dissipating member 23c surrounding the outer surface of the heat conductive tube 23b. ), The temperature sensor 23d installed on the heat dissipating member 23c, the silver foil 23e surrounding the heat dissipating member 23c, and the engaging portion 23a of the lower end portion of the heat conductive pipe 23b. Combined heat conduction plates 23f and 23f ', a plate cooler 23g inserted into and fixed to an inner central portion of the heat conduction plate 23f', and a plurality of annular heat sinks installed on the outer surface of the heat conduction plate 23f '. It consists of the swash plate 23h.

Here, the temperature control range of the cooling means 23 can be arbitrarily set, and is usually set in the range of 0 ° C to 10 ° C.

The cooling block 27 'is a block of a square column having a plurality of cylindrical holes 27a' and is inserted into the heat conduction tube 23b, and the heating block 27 and the cooling block 27 'are the same. May be used, or different ones may be used in which the formation of the cylindrical holes 27a and 27a 'is different.

Next will be described in detail the operation of the mixer combined centrifuge having a temperature control function of the present invention configured as described above.

First, if you want to control the temperature of the temperature control object (hereinafter referred to as "sample") put in various tubes or syringes using the temperature control means 20 of the present invention, the first and second heating means according to the temperature to be adjusted 22, 22 'or cooling means 23 are selected.

That is, when the temperature to be adjusted is 80 ° C. or higher and 95 ° C., the first heating means 22 is lower than 65 ° C. or higher and lower than 80 ° C., and the second heating means 22 ′ is 0 ° C. to 10 ° C. and cooling means ( 23).

For example, if the sample contained in the syringe is to be maintained at 90 ° C., the first heating means 22 is selected to open the lid 26 of the temperature adjusting means 20, and the heat conduction of the first heating means 22 is selected. Insert the syringe into the cylindrical hole 27a of the heating block 27 inserted into the tubular body 22b, close the lid 26, and operate the control panel 28 to set the temperature to 90 deg. Activate the means 22.

Accordingly, the plate heater 22g of the first heating means 22 is heated to maintain the temperature inside the cylindrical hole 27a of the heating block 27 to 90 ° C, and this temperature is maintained by the temperature sensor 22d. It detects in real time and is transmitted to the microprocessor embedded in the control panel 28. When the temperature detected by the temperature sensor 22d is less than 90 ° C, the plate heater 22g is operated under the control of the microprocessor. The control of the processor stops the operation of the plate heater 22g, so that the temperature inside the cylindrical hole 27a of the heating block 27 is kept constant at 90 ° C, so that the sample contained in the syringe maintains the temperature of 90 ° C.

If the sample contained in the tube is to be maintained at 70 ° C, the second heating means 22 'is selected to open the lid 26 of the temperature control means 20, and the second heating means 22' thermally conductive tube 22b. The tube is inserted into the cylindrical hole 27a of the heating block 27 inserted in the heating block 27, the lid 26 is closed, and the control panel 27 'is operated to set the temperature to 70 deg. 22 ').

Accordingly, the plate heater 22g of the second heating means 22 'is heated to maintain the temperature inside the cylindrical hole 27a of the heating block 27 to 70 ° C, and this temperature is maintained at the temperature sensor 22d. Is detected in real time and transmitted to the microprocessor embedded in the control panel 28 'so that when the temperature detected by the temperature sensor 22d is less than 70 ° C, the plate heater 22g is operated under the control of the microprocessor, and the temperature exceeds 70 ° C. When the plate heater 22g is stopped under the control of the microprocessor, the temperature inside the cylindrical hole 27a of the heating block 27 is kept constant at 70 ° C., so that the sample contained in the tube maintains the temperature of 70 ° C. do.

In addition, in order to maintain the sample contained in the syringe at 8 ° C., select the cooling means 23 to open the lid 26 of the temperature control means 20, and insert it into the heat conduction tube 23 b of the cooling means 23. Insert the syringe into the cylindrical hole 27a 'of the cooling block 27', close the lid 26, operate the control panel 28 " to set the temperature to 8 DEG C, and set the cooling means 23. It works.

Accordingly, the plate cooler 23g of the cooling means 23 is cooled to maintain the temperature inside the cylindrical hole 27a 'of the cooling block 27' by lowering to 8 ° C, and this temperature is maintained by the temperature sensor 23d. When detected in real time and transmitted to the microprocessor embedded in the control panel 28 ", when the temperature detected by the temperature sensor 23d is less than 8 ℃, the operation of the plate cooler (23g) is stopped by the control of the microprocessor, 8 ℃ If exceeded, the plate cooler (23g) is operated under the control of a microprocessor, so that the temperature inside the cylinder hole (27a ') of the cooling block (27') is kept constant at 8 ° C, so the sample contained in the syringe maintains the temperature of 8 ° C. Done.

On the other hand, when centrifugation is desired, the object to be centrifuged (hereinafter referred to as "sample") is put in the tube (X), and the power is supplied to the centrifuge equipped with the mixer of the present invention by operating a switch on the control panel (5). .

Depending on the type of tube X used here, as shown in FIG. 6, one bucket 2a may be inserted, or two or more buckets 2a may be inserted.

Then, the user opens the lid 1, and the lower end of the tube (X) held by the hand touches the vibrating cup 13 of the mixer 10 and presses the vibrating cup 13, the operation of the motor 11 is switched As the eccentric shaft 12 of the motor 11 rotates eccentrically, the vibrating cup 13 vibrates, and thus, the tube X also vibrates and the sample in the tube X is mixed.

After mixing the sample by the mixer 10, the user inserts the tube X into the bucket 2a of the centrifuge means 2, closes the lid part 1, and then puts it in the control panel 5. Operate the switch to operate the centrifuge.

Then, the motor 11 operates to rotate the bucket frame 2b of the centrifugal separation means 2 so that the bucket 2a into which the tube X is inserted rotates so that the sample in the tube X receives the centrifugal force. Centrifuged, if necessary, the centrifuged tube (X) may be mixed again using the mixer (10).

Here, the rotational speed of the motor can be arbitrarily adjusted in the range of 300 to 11,000 rpm according to the set value set in the microprocessor in the control panel 5, and if necessary, the rotation of the motor is divided into a low speed step and a high speed step. The sample can be centrifuged while controlling the speed.

While the present invention has been described as a preferred embodiment, the present invention is not limited thereto, and various modifications can be made without departing from the spirit of the invention.

Figure 1 is a perspective view of the lid open state of the mixer combined centrifuge having a temperature control function of the present invention,

Figure 2 is a perspective view of the lid open state of the mixer centrifuge with a temperature control function of the present invention showing another embodiment of the centrifuge,

3 is an exploded perspective view of a heating means and a heating block according to the present invention;

4 is an exploded perspective view of a cooling means and a cooling block according to the present invention;

5 is a cross-sectional view taken along the line A-A of FIG.

6A is a cross-sectional view taken along the line BB of FIG. 1,

6B is a cross-sectional view taken along the line C-C of FIG.

7 is a perspective view showing the various structures of the bucket insert employed in the present invention,

8 is a state diagram used in the heating block and the cooling block according to the present invention.

Description of the Related Art

1: lid 2: centrifugation means

2a: Bucket 2a ': Rotating protrusion

2b: Bucket Frame 2b ': Bucket Insert

2c: Inclined groove 2d: Angle rotor

2d ': Bucket insert groove 3: Motor means

4: buffer means 5: control panel

6: main body 6a: discharge port

6b: inner case 10: mixer

11 motor 12 eccentric shaft

13: vibrating cup 20: temperature control means

21 body portion 22 first heating means

22 ': second heating means 22a, 23a: coupling portion

22b, 23b: heat conduction tube 22c, 23c: heat dissipating material

22d, 23d: temperature sensor 22e, 23e: silver foil

22f, 22f ', 23f, 23f': Heat Transfer Plate 22g: Plate Heater

23: cooling means 23g: plate cooler

23h: annular thermal radiation plate 24: cover body

25: lid mount 26: lid

26a: hinge 27: heating block

27 ': Cooling block 28, 28', 28 ": control panel

Claims (9)

A plate-shaped lid portion 1 composed of a translucent body, centrifugal separation means 2 provided with a plurality of bucket frames 2b having a 'Y' shape at the center thereof, and a lower portion of the centrifugal separation means 2; A motor means (3) installed in the rotary means for rotating the centrifugal separation means (2), a buffer means (4) for reducing noise and vibration of the motor means (3), and a microprocessor for controlling the motor means (3) A control panel (5) having a built-in, and an inner case (6b) formed with a plurality of discharge ports (6a) to discharge the air generated by the centrifugal separation means (2) to the outside and the control panel (5) in front A centrifuge comprising a main body portion (6) provided with a main body (6), the mixer (10) being built up and down in the front corner portion of the main body portion (6) and connected to one side of the main body portion (6); Having a temperature control function characterized in that it further comprises a temperature control means 20 is installed Combined centrifuges. According to claim 1, wherein the centrifugal separation means (2) is a central portion is fixed to the axis of the motor means (3) a plurality of bucket frame (2b) of the 'Y' shape from the center is similarly formed, the bucket frame The inclined groove (2c) is formed at the end of the (2b) and the inclined groove (2b) with the upper portion of the bucket (2a) is fixed to the bucket insert (2b ') formed on the outer periphery, the rotary projection (2a') A mixer-use centrifuge having a temperature control function, characterized in that a rotary projection (2a ') is inserted into 2c). The angle rotor (2d ') is characterized in that a plurality of bucket insertion groove (2d) formed downward along the edge of the cylindrical body instead of the plurality of' Y 'shaped bucket frame (2b) Combined centrifuge with temperature control. The funnel-shaped vibration cup according to claim 1, wherein the mixer (10) is fitted with a motor (11) for rotating the eccentric shaft, the eccentric shaft (12) of the motor (11), and the eccentric shaft (12). The vibration cup 13 is configured to oscillate by the eccentric rotation of the eccentric shaft 12 in accordance with the operation of the motor 11 is operated by switching to the downward pressing of the vibration cup 13 made of (13) Combined centrifuge with a temperature control function, characterized in that there is. The method of claim 1, wherein the temperature control means 20 is the first heating means mounted to the rear portion of the body portion 21 to have a body portion 21 having a hexahedral shape, and an opening on the upper surface of the body portion 21 (22) and the second heating means (22 '), the cooling means 23 is mounted in the center of the first half of the body portion 21 to have an opening on the body portion 21, the first and the first and second heating means A cover body 24 having an open upper surface of the upper surface of the upper surface of the body portion 21 on which the 22 and 22 'are mounted; A lid mounting stand 25 in which a rear plate which stands on the rear end of the upper surface of the body portion 21 and a side plate which is installed on both sides of the rear half portion are integrally formed, and an upper end of the rear plate forms a lid mount; A lid 26 installed on the lid mounting stand 25 by a hinge 26a; A heating block 27 or a cooling block 27 'inserted into the first and second heating means 22 and 22' and the cooling means 23; Temperature control, characterized in that composed of a control panel (28, 28 ', 28 ") having a microprocessor for controlling the temperature of the first and second heating means (22, 22') and the cooling means (23) Combined centrifuge with a function. The temperature control range of the first heating means 22 is 80 ° C or more and 95 ° C, and the temperature control range of the second heating means 22 'is 65 ° C or more and less than 80 ° C. Mixer combined centrifuge having a temperature control function, characterized in that the temperature control range of the means (23) is 0 ° C to 10 ° C. The method of claim 5, wherein the first and second heating means (22, 22 ') each of the square pipe-type heat conduction tube (22b) and the heat conduction of the upper and lower front end extending in the right, left, right and right directions to form a coupling portion (22a), The heat dissipation material 22c surrounding the outer surface of the tubular body 22b, the temperature sensor 22d embedded in the heat dissipation material 22c, the silver foil 22e surrounding the heat dissipation material 22c, and the It consists of heat conduction plates 22f and 22f 'coupled to the coupling part 22a of the lower end part of the heat conduction tube 22b, and the plate heater 22g inserted and fixed between the heat conduction plates 22f and 22f'. Mixer combined centrifuge having a temperature control function, characterized in that. 6. The cooling means (23) according to claim 5, wherein the cooling means (23) has a rectangular pipe-type heat conducting tube (23b) having an upper and lower front end extending in right, left, right and right directions to form a coupling portion (23a), and an outer surface of the heat conducting tube (23b). A surrounding heat dissipating member 23c, a temperature sensor 23d installed on the heat dissipating member 23c, a silver foil 23e surrounding the heat dissipating member 23c, and a lower end portion of the heat conducting tube body 23b. The heat conduction plates 23f and 23f 'coupled to the coupling portion 23a of the plate, the plate cooler 23g inserted and fixed to the inner central portion of the heat conduction plate 23f', and the outer surface of the heat conduction plate 23f '. Mixer combined centrifuge having a temperature control function, characterized in that composed of a plurality of annular heat radiation plate (23h) is installed as a plate. 9. The heating block 27 according to claim 7 or 8, wherein the heating block 27 is a block of a square column having a plurality of cylindrical holes 27a, and is inserted into the heat conduction tube 22b, and the cooling block 27 ' The mixing and centrifugal mixer with a temperature control function, characterized in that the block body of the rectangular column formed with a plurality of cylindrical holes (27a ') is inserted into the heat conduction tube (23b).

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