KR20100125171A - Soluble myostatin prodomain recombination protein having myostatin inhibitory activity and its use - Google Patents

Abstract

PURPOSE: A soluble myostatin prodomain recombinant protein having activity of suppressing myostatin is provided to induce double-muscle or low fat higher organism and marine organism and to improve muscle growth. CONSTITUTION: A method for producing soluble myostatin prodomain recombinant protein comprises: a step of cloning the myostatin prodomain gene using myostatin of tissue; a step of inserting the prodomain to an expression vector; a step of transforming the prodomain into E. coli and adding expression inducer to expressing recombinant protein of the soluble myostatin prodomain; and a step of purifying the recombinant protein of the transformed soluble myostatin prodomain. A pharmaceutical composition for preventing and treating muscular diseases contains the recombinant protein as an active ingredient.

Description

Soluble myostatin prodomain recombination protein having myostatin inhibitory activity and its use}

The present invention relates to a soluble myostatin prodomain recombinant protein having myostatin inhibitory activity and its use. More specifically, the present invention relates to a soluble myostatin that inhibits the function of myostatin prepared using various expression vectors and E. coli. The present invention relates to a recombinant protein of ostatin prodomain and a feed additive for promoting muscle growth or improving a growth rate of plants and animals using the same, or to prevent and treat muscle-related diseases such as muscular dystrophy or muscular dystrophy.

Myostatin, a muscle growth regulator, is also called a growth and differentiation factor (GDF-8), regulates cell proliferation and differentiation, and belongs to the TGF-b family. In mice that are knocked out of myostatin, muscles are increased 2-3 times than normal mice, and recently, it is known that muscle accumulation increases and fat accumulation decreases.

Myostatin of the higher organisms known to date is very similar in structure. In general, myostatin is composed of three exons and two introns, and the myostatin gene is about 3.1 kb. RNA is known to exist in and most muscles. Myostatin mRNA produces a myostatin protein consisting of about 375 amino acids, and myostatin protein is largely divided into three parts: a signal peptide region, a propeptide (prodomain) region; 28 kDa, N-terminus] and mature region (12 kDa, C-terminus). Myostatin undergoes three major post-translational processing in cells. The propeptide and the mature moiety are disulfide bonds with each other, thereby maintaining the shape of the homer dimeric protein. As the proteolytic part is cleaved, it is divided into a propeptide zone and a mature zone. After cleavage, the propeptide region binds to the mature region via non-covalent binding. Then, as it is secreted out of the cell, the mature zone binds to and becomes phosphorylated with activin type II receptors, passing its signal back to the activin type I receptor, Signals are delivered to receptor-regulated proteins Smad2 and Smad3, and Smad2 and Smad3 bind co-Smad4 to regulate transcription of the gene of interest.

Most of the studies on myostatin are limited to higher animals, and recently, research has begun as the presence of myostatin in marine animals is known. Myostatin research shows that by inhibiting the function of myostatin, a large amount of muscle production and low fat, ie, double-muscle individuals, are rapidly reduced in weight, such as AIDS patients. Research into the mechanism of myostatin is being used, such as regeneration of myotubes and muscle atrophy of unused muscles.

Research on how to regulate the amount of muscle by offsetting the function of myostatin is as follows. According to research by Yang et al. And Lee and McPherron, overexpression of the myostatin propeptide region results in the myostatin propeptide region binding to the myostatin mature peptide. The activity of statin-maturated peptides was lost and muscle mass increased. As a result of this and Macperon's overexpression of activin receptors, myostatin mature peptides bind to activin receptors and lose their function, resulting in hyperplasia (27%) and hypertrophy (19%). Appeared, resulting in an increase in muscle. Recently, Carpio et al. Induced the growth of muscle using recombinant activin IIB.

In the case of metalloproteinases belonging to the BMP-1 / Tolloid group, it is known that myostatin substantially loses its function by degrading all of the propeptide regions of myostatin. In the case of follistatin, when a protein belonging to the TGF-b family is secreted, it is reported that the muscle increases due to the failure to control the growth of the muscle by binding. Anti-myostatin monoclonal antibodies against the mature myostatin of chickens were produced and injected directly into the fertilized eggs of the chickens, and the weight and muscle mass of the chickens were measured. Increased. Callis et al. Reported that microRNAs play an important role in muscle development.

In the cell surface expression vector expressing myostatin and the microorganism transformed by the vector, polynucleotide encoding myostatin mature protein and polygamma glutamic acid inducing surface expression of the microorganism A feed additive or vaccine comprising a cell surface expression vector containing a gene encoding an enzyme complex, a microorganism transformed with the vector and a myostatin mature protein produced by culturing the transformed microorganism as an active ingredient are disclosed. The Korean patent application No. 10-2008-7008707 anti-myostatin antibody is characterized by high affinity useful for increasing muscle mass, increasing bone density, or treating various disorders in mammalian and avian species. , Chimeric, humanized or fully human antibodies, immunoconjugates of antibodies or antigen-binding thereof An anti-myostatin antibody is disclosed that can be a combined fragment.

Most of the methods that counteract myostatin function focus on higher animals. In the case of marine organisms, it is necessary to mass-produce double-muscled marine organisms by inhibiting the function of myostatin, so it is difficult to apply existing methods (genes such as RNAi, recombinant proteins using mammalian cell lines, etc.). It is almost meaningless in economic terms. Therefore, we need to find an easier and more economical way to apply to marine life.

Development of methods for inhibiting myostatin function involves the production of recombinant proteins from monoclonal antibodies of myostatin and mammalian cells and the inhibition of the function of mature myostatin in higher organisms and oceans that require mass production. Application to living things is inadequate in economics. Therefore, it is necessary to develop an easier and more economical method for mass production.

In order to solve this problem, the present invention used prodomain recombinant protein, which is one of the methods of inhibiting the function of myostatin. In general, myostatin has 7-9 cysteines, and thus, mammalian cells are used to produce recombinant proteins having the function of myostatin. However, in order to apply the inclusion body produced from Escherichia coli, it must go through a refolding process. However, the present inventors have developed a method for producing recombinant proteins of soluble myostatin prodomains that inhibit the function of myostatin using various expression vectors and E. coli, and thus, marine animals, including higher animals, higher plants, and zooplankton. Applied to marine plants, including phytoplankton, it was confirmed that the present invention can be applied to dual muscle-forming higher organisms, marine organisms and medicines, and completed the present invention.

Accordingly, an object of the present invention is to provide a recombinant protein production method of soluble myostatin prodomains that inhibits the function of myostatin using various expression vectors and E. coli.

It is another object of the present invention to provide a recombinant protein of soluble myostatin prodomain that inhibits the function of myostatin produced by the above method.

It is another object of the present invention to provide a feed additive for promoting muscle growth or improving the growth rate of a flora and fauna containing a recombinant protein of soluble myostatin prodomain or an agent for preventing and treating muscle related diseases such as muscular dystrophy or muscular dystrophy.

An object of the present invention as described above is to clone the halibut myostatin prodomain gene using PCR from myostatin cDNA of halibut muscle tissue as a biological myostatin prodomain, and insert it into the expression vectors pET32a and pMALc2x. E. coli Rogetta-gami2 (DE3) pLyss effectively expressed soluble myostatin prodomain, a water-soluble protein, and was purified using metal binding affinity and amylose resin affinity chromatography. , 30 ug of purified soluble myostatin prodomain, respectively, from 250 mL of culture.

The present invention provides a method for cloning a myostatin prodomain gene using myostatin of a biological tissue, inserting the biomyostatin prodomain into an expression vector, and expressing the expression vector-binding myostatin prodomain in E. coli. Transforming to express a recombinant protein of soluble myostatin prodomain, and purifying the recombinant protein of the transformed soluble myostatin prodomain, and the soluble myostatin pro inhibiting the function of myostatin. It provides a method for producing a recombinant protein of the domain.

In the present invention, the myostatin prodomain is preferably a cow, a rat, for example, a rat and a mouse, a pig, a chicken, a turkey, a sheep, a human, a dog, a mallard, a quail, a monkey, for example, the Philippines. Terrestrial animals such as monkeys and roland gorillas, abalones, flounders, e.g. stone flounder, flounder, zodiac rock, tilapia, sea bream, e.g. sea bream and black sea bream, puffer fish, e.g. trout, salmon, catfish, flounder Myostatin prodomains of marine animals such as rotifers.

In the present invention, the expression vector may be selected from the group consisting of pET32a, pET43.a, pMALp2x and pMALc2x, preferably pET32a and pMALc2x.

In the present invention, the E. coli may be selected from the group consisting of BL21 (DE3), BL21 (DE3) pLyss, origami2 (DE3), Origami2 (DE3) pLyss, and Rogetta-gami2 (DE3) pLyss, preferably Rogetta- gami2 (DE3) pLyss.

The present invention provides a recombinant protein of soluble myostatin prodomains that inhibits the function of myostatin of an organism produced by the above method.

An organism capable of inhibiting myostatin function using the recombinant protein of the soluble myostatin prodomain according to the present invention may be any living organism on the earth, and in particular, a superhero animal belonging to a higher animal, for example, an invertebrate animal. , Marine animals, including toxins, fishes, amphibians, reptiles, birds, mammals, etc. belonging to vertebrates, tonics, tortoises, molluscs, arthropods, and vertebrates, including, for example, flowering plants and zooplankton And all marine plants, including phytoplankton.

The recombinant protein of soluble myostatin prodomains that inhibits the function of myostatin of the organism according to the present invention is preferably a pMALc2x-prodomain protein or a pET32a-prodomain protein.

Pro domains of pMALc2x-prodomain protein or pET32a-prodomain protein that inhibit the function of myostatin in organisms according to the present invention may be used in cattle, rats, mice, pigs, chickens, turkeys, sheep, humans, dogs, mallards, It may be a quail, Philippine monkey, Roland gorilla, abalone, stone flounder, flounder, zodiac rock, tilapia, red snapper, black sea bream, purple lobster, rainbow trout, salmon, catfish, rotifer or flounder myostatin prodomain.

The myostatin prodomain according to the present invention is described in SEQ ID NOs: 1-26.

The pMALc2x-prodomain protein that inhibits the function of myostatin of the organism according to the present invention may be any one of the pMALc2x-prodomain proteins of SEQ ID NOs: 27 to 52.

The pET32a-prodomain protein that inhibits the function of myostatin of the organism according to the present invention may be any one of the pET32a-prodomain proteins of SEQ ID NOs: 53-78.

The present invention provides a feed additive composition for promoting muscle growth or improving the growth rate of a flora and fauna containing the recombinant protein of the soluble myostatin prodomain.

In the present invention, the feed additive is a generic term for substances added in a small amount to the feed for nutritional or specific purposes of animals and plants.

The present invention provides a pharmaceutical composition for the prevention and treatment of muscle diseases, such as muscular dystrophy, muscular dystrophy, muscle loss disease, etc. containing a recombinant protein of soluble myostatin prodomain as an active ingredient to inhibit the activity of myostatin.

The present invention provides a health food composition for improving muscle growth containing the recombinant protein of the soluble myostatin prodomain as an active ingredient.

In the present invention, the term "active ingredient" refers to a substance or a group of substances (including medicinal herbs whose pharmacologically active ingredients are not known) which are expected to directly or indirectly express the efficacy effect of the medicine by inherent pharmacological action. It means containing a main component as.

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

Dosage forms of the pharmaceutical compositions of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable collection.

"Health functional food" as defined in the present invention means a food manufactured and processed using raw materials or ingredients having functional properties useful for the human body according to the Health Functional Food Act No. 6767, and "functional" means a human body It means the ingestion for the purpose of obtaining a useful effect in health use such as nutrient control or physiological action on the structure and function of.

Recombinant pMALc2x-prodomain protein and pET32a-prodomain protein according to the present invention showed an inhibitory effect on myostatin activity through luciferase analysis, and the recombinant soluble myostatin prodomain thus expressed is higher animals, higher plants. In addition, marine animals, including zooplankton, and marine plants, including phytoplankton, may be used to inhibit myostatin, thereby inducing double-muscle or low-fat higher organisms and marine organisms, and in medicine for muscle-related diseases. It is feed.

In addition, feed additives for promoting muscle growth or improving body weight of plants and animals containing the active ingredient of E. coli expressing soluble myostatin prodomain may also be used for the prevention and treatment of muscle-related diseases such as muscular dystrophy and muscular dystrophy.

Myostatin prodomain recombinant proteins pMALc2x-prodomain protein and pET32a-prodomain protein according to the present invention has an inhibitory effect on myostatin activity, marine animals, including higher animals, higher plants and zooplankton, It can be applied to marine plants containing phytoplankton to inhibit the function of myostatin to induce double-muscle or low fat higher organisms and marine organisms, and to promote muscle growth of plants and animals by using soluble myostatin prodomain recombinant protein. Alternatively, the present invention may be used for the preparation of a feed additive for improving the growth rate, or for the preparation and treatment of muscle-related diseases such as muscular dystrophy or muscular dystrophy.

1 is a diagram showing the expression results of pET32a poMSTN prodomain induced in E. coli strain Rogetta-gami2 (DE3) pLyss.
Figure 2 is a diagram showing the expression results of pMALc2x poMSTN prodomain induced in E. coli strain Rogetta-gami2 (DE3) pLyss.
Figure 3 is a diagram showing the expression results of pET32a prodomain (1), pMALc2x prodomain (2), pET43.1a prodomain (3) induced in E. coli strain Rogetta-gami2 (DE3) pLyss at 37 degrees.
Figure 4 shows the expression results of pET32a prodomain (1), pMALc2x prodomain (2), pET43.1a prodomain (3) induced in E. coli strain Rogetta-gami2 (DE3) pLyss at 12.5 degrees.
5 is a diagram showing the results of expression over time after IPTG addition of pET32a poMSTN prodomain induced in E. coli strain Rogetta-gami2 (DE3) pLyss.
6 is a diagram showing the results of expression over time after IPTG addition of pMALc2x poMSTN prodomain induced in E. coli strain Rogetta-gami2 (DE3) pLyss.
Figure 7 shows the affinity chromatography results for pET32a poMSTN prodomain in E. coli strain Rogetta-gami2 (DE3) pLyss.
FIG. 8 shows affinity chromatography results for pMALc2x poMSTN prodomain in E. coli strain Rogetta-gami2 (DE3) pLyss.
Figure 9 shows the results of size exclusion chromatography of the pET32a poMSTN prodomain.
10 is a diagram showing the size exclusion chromatography results of pMALc2x poMSTN prodomain.
11 is a diagram showing the expression and purification results of pET32a poMSTN prodomain induced in E. coli strain Rogetta-gami2 (DE3) pLyss.
12 is a diagram showing the results of expression and purification of pMALc2x poMSTN prodomain induced in E. coli strain Rogetta-gami2 (DE3) pLyss.
13 is a diagram showing inhibition of myostatin activity by recombinant prodomains (pET32a-prodomain and pMALc2x-prodomain) (A) and (B).
14 is a diagram showing the weight variation and biochemical analysis by myostatin activity inhibition after applying recombinant prodomains (pET32a-prodomain and pMALc2x-prodomain) to fish for one month.

Hereinafter, preferred embodiments of the present invention will be described in more detail with reference to Examples. However, the scope of the present invention is not limited to these examples.

Example

LB liquid medium was prepared with 1.2% tryptone, 0.6% yeast extract, and 0.8% NaCl at pH 7.0. Antibiotic plate medium for selection of strains was BL21 (DE3) Escherichia coli, and the final concentration of ampicillin (Amp, Sigma) was 50. ug / mL and Origami2 (DE3) Escherichia coli were prepared with ampicillin (Amp, Sigma) and tetracycline (tetracycline; Tet, Sigma) at a final concentration of 50 ug / mL and 12.5 ug / mL, respectively. (DE3) pLyss and Origami2 (DE3) pLyss and Rogetta-gami2 (DE3) pLyss Escherichia coli have a final concentration of 50 μg / mL and 34 ug of ampicillin (Amp, Sigma) and chloroamphenicol (Cam, Sigma), respectively. It prepared to be / mL.

Protein expression strains and protein expression plasmids used in this Example are shown in Table 1 below.

TABLE 1

Vector, expressed structure, detected MW, promoter, strain and place of purchase of this example

Escherichia coli BL21 (DE3), BL21 (DE3) pLyss, origami2 (DE3), Origami2 (DE3) pLyss and Rogetta-gami2 (DE3) pLyss were purchased from Novagen. pET32a and pET43.a were purchased from Novagen and pMALp2x and pMALc2x were purchased from New England BioLab.

Example 1 Cloning of Flounder Myostatin Prodomain Gene and Preparation of an E. Coli Expression Vector

Total RNA was extracted from halibut muscle tissue using the Trizol reagent method (Invitrogen, USA). CDNA was synthesized using Superscript ™ II Reverse Transcriptase (Invitrogen) with 0.5 ug of total RNA as a template.

Oligonucleotide primers synthesized based on the nucleotide sequence of the olive flounder myostatin gene (GeneBank Accession No. DQ412048) ') Was amplified for the myostatin prodomain using an iCycler thermal cycler (Bio-Rad, USA). At this time the protein, the restriction position of the side of the primer is EcoR I, 3 'expression vector pET32a, pET43.1a, pMALp2x and pMAL to c2x vector using the 5-side of the primer contains a restriction enzyme and the termination codon position of the Hind III Designed. The composition of the polymerase chain reaction (PCR) reaction mixture consisted of 2 μl cDNA, 2.5 μl 10x buffer, 1 μl 25 mM MgCl ₂ , 0.5 μl 10 mM dNTP, 0.5 μl 10 pM forward primer, 0.5 μl 10 pM reverse primer, GoTaq (5 U / μl, promega) 0.1 μl and DDW 18.4 μl were added to make the total volume 25 μl. The PCR reaction was carried out for 35 cycles of 94 ° C. 3 minutes [pre-denaturation], 94 ° C. 40 seconds, 56 ° C. 40 seconds, 72 ° C. for 1 minute, and at 10 ° C. for 10 minutes at final extension. . After the reaction was confirmed and separated by 1.0% agarose gel electrophoresis. Genes isolated from the gel were purified by gel cleaning kit (Bioneer).

When the flounder myostatin gene was amplified by PCR using the flounder muscle cDNA template, agarose gel electrophoresis resulted in DNA bands of about 800 nucleotides in size. The E. coli expression system of the cloned flounder myostatin gene was prepared as follows. Reaction of pMALp2x and pMALc2x with E. coli expression vectors pET32a, pET43.1a, and tac promoters with the prodomain region and T7 promoter of the flounder myostatin gene amplified by PCR was performed at 37 degrees for 3 hours at 37 ° C with restriction enzymes EcoR I and Hind III After cutting, the mixture was separated by agarose gel electrophoresis. The isolated vector and the structural gene were linked using T4 DNA ligase to prepare an expression plasmid of recombinant myostatin. The resulting pET32a-prodomain, pET43-prodomain, pMALp2x-prodomain and pMALc2x-prodomain were transformed with E. coli DH5α. From the cultured cells of transformed DH5α, pET32a-prodomain, pET43-prodomain, pMALp2x-prodomain and pMALc2x-prodomain plasmid were obtained. Expression vectors obtained at this time were identified by agarose gel electrophoresis and DNA sequencing to identify myostatin prodomains.

Example 2 Preparation of Expression Vectors and Expression of Recombinant Flounder Myostatin Prodomains

The purified halibut myostatin prodomain region gene and protein expression vectors pET32a, pET43.1a, pMALp2x and pMAL c2x were digested with restriction enzymes EcoR I (promega) and Hind III (promega) at 37 ° C for 3 hours, followed by T4. Recombinant myostatin prodomain expression plasmid was prepared using ligase.

Escherichia coli BL21 (DE3), BL21 (DE3) pLyss, Origami2 (DE3), Origami2 (DE3) pLyss and Rogetta-gami2 transformed with pET32a-prodomain, pET43-prodomain, pMALp2x-prodomain and pMALc2x-prodomain DE3) pLyss (amp 50 ug / mL), (amp 50 ug / mL, cam 34 ug / mL), (amp 50 ug / mL), (amp 50 ug / mL, cam 34 ug / mL), (amp Isopropyl-beta-D-thiogalactopyranoside, an expression inducer, when expressed at 37 ° C OD600 0.4-0.5 in LB medium containing 50 ug / mL, cam 34 ug / mL) IPTG, Bioneer) was incubated at a final concentration of 0.2 mM and incubated at 37 ° C, 30 ° C, 18 ° C and 12.5 ° C for 4 hours, 10 hours, 16 hours and 24 hours, respectively, and centrifuged at 10,000 g for 20 minutes. .

Escherichia coli BL21 (DE3), BL21 (DE3) pLyss, Origami2 (DE3), Origami2 (DE3) pLyss and Rogetta-gami2 transformed with pET32a-prodomain, pET43-prodomain, pMALp2x-prodomain and pMALc2x-prodomain Cells obtained by small incubation (5 mL) of DE3) pLyss were collected by centrifugation at 10,000 g for 20 minutes and stored at -20 ° C until purification.

To purify the proteins contained in the transformed pET32a-prodomain, pET43-prodomain, pMALp2x-prodomain and pMALc2x-prodomain aqueous fractions, they were dissolved in 20 mM Tris-HCl (pH7.5), 200 mM NaCl buffer. After crushing the cells using an ultrasonic crusher (sonicator, misonic INC) to obtain an aqueous fraction by centrifugation at 10,000g for 15 minutes, the presence or absence of soluble myostatin prodomain using SDS-PAGE (Table 1) 2).

TABLE 2

Vectors, Host Cell Strains, and Induction Temperature of Vectors to Obtain Soluble poMSTN Prodomains

+: Recombinant soluble prodomain protein is expressed

-: Recombinant soluble prodomain protein is not expressed

±: co-expression of recombinant soluble prodomain protein and inclusion body (insoluble, inclusion body)

E. coli BL21 (DE3), BL21 (DE3) pLyss, Origami2 (DE3), Origami2 (DE3), Origami2 (DE3), Origami2 (DE3), Origami2 (DE3), Origami2 (DE3), Origami2 (DE3) DE3) pLyss and Rogetta-gami2 (DE3) pLyss were transformed, and then cultured at 37 ° C. for about 3-4 hours in 5 mL of LB medium. When the OD600 0.4-0.5 of the culture solution, the protein inducer IPTG was administered to a final concentration of 0.2 mM to induce protein expression at 37 ° C. for 4 hours, 30 ° C. for 10 hours, 18 ° C. for 12 hours, and 12.5 ° C. for 24 hours. As a result, it was confirmed that water-soluble proteins were expressed in pET32a-prodomain and pMALc2x-prodomain transformed in Rogetta-gami2 (DE3) pLyss (see Table 2 and FIGS. 3 and 4).

The molecular weights of the recombinant olive flounder myostatin protein expressed by the expression vectors pET32a-prodomain and pMALc2x-prodomain were identified at 54 kDa and 80 kDa positions in SDS-PAGE, respectively (see FIGS. 1 and 2). In order to set the incubation time after IPTG administration, the expression levels of recombinant halibut myostatin according to each time were examined after culturing for 12 hours, 24 hours, 36 hours, and 48 hours. As a result, the expression amount increased up to 24 hours, but when cultured for 48 hours, the expression amount gradually decreased, and there was almost no difference in the expression amount between 24 hours and 36 hours (see FIGS. 5 and 6). Therefore, the optimum expression condition of recombinant halibut myostatin in Escherichia coli was optimally incubated at 12.5 degrees for 24 hours after administration of 0.2 mM IPTG, a protein inducer, when the culture medium had an OD600 of 0.4-0.5.

In this example, IPTG was used as the expression inducing agent, but Lactose of 0.001-10% may be used, and the temperature range is described as 12.5 ° C.-37 ° C. in this example, but 0 ° C.-15 ° for pET32a-prodomain. In the case of ° C and pMALc2x-prodomain, water-soluble proteins were expressed at 0 ° C to 50 ° C, respectively. In addition, in the present embodiment, the concentration of IPTG was 0.2 mM as the expression inducing agent, but depending on the experimental method, it may be used up to 0.001-10 mM.

In the present embodiment, expression vectors and the like were transformed in E. coli, but it is obvious that even if transformed in yeast or the like falls within the scope of the present invention.

Example 3 Purification of Recombinant Flounder Myostatin Prodomains

Cells obtained by culturing (250 mL) E. coli Rogetta-gami2 (DE3) pLyss transformed with pET32a-prodomain and pMALc2x-prodomain were centrifuged at 4,000 g for 30 minutes and stored at -20 ° C until purification.

Immobilized metals in which the recombinant protein solution was equilibrated with 20 mM phosphate (pH8.0), 300 mM NaCl, 10 mM imidazole buffer solution for purification of the protein contained in the transformed pET32a-prodomain aqueous fraction. affinity) column (Ni-NTA agarose, Qiagen). Wash thoroughly with 20 mM phosphate (pH 8.0), 300 mM NaCl, 10 mM imidazole buffer to remove unnecessary protein in the column, then 20 mM phosphate (pH 8.0) containing 30 mM imidazole, Eluted with 300 mM NaCl buffer (see FIG. 7).

Recombinant protein solutions were adsorbed onto amylose resin (New England BioLab) equilibrated with 20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA buffer for protein purification in the transformed pMALc2x-prodomain water soluble fraction. Wash thoroughly with 20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA buffer to remove unnecessary protein in the column, then 20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA buffer containing 10 mM maltose. Eluted (see FIG. 8).

Size exclusion gel chromatography (SEGC) was TSK G3000 column (Tosho), equilibrated with 20 mM Phosphate buffer (pH8.0) and 300 mM NaCl, and the elution rate was 0.3mL / min with the same solution. The absorbance was measured to collect fractions by 0.2 mL (see FIGS. 9 and 10).

Purification of recombinant pET32a-prodomain and pMALc2x-prodomain myostatin proteins using a nickel affinity column was confirmed by SDS-PAGE, and the bands were identified at about 54 kDa and 80 kDa. The recovery of pET32a-prodomain and pMALc2x-prodomain against total water soluble protein after affinity chromatography was about 1.5% and 1.8%, respectively, and the total water soluble protein of pET32a-prodomain and pMALc2x-prodomain after size exclusion gel chromatography. The recovery rates were 0.6% and 0.8%, respectively (see Table 3, FIGS. 11-12).

TABLE 3

Yield and recovery of soluble pET32a poMSTN and pMALc2x poMSTN prodomains during the purification step

^a Protein concentration was determined by Bradford method using BSA as a standard.

Example 4 Determination of Myostatin Inhibitory Activity of Recombinant pET32a-Prodomain and pMALc2x-Prodomain Protein

In vitro analysis showed that A204 cells were seeded with 30,000 cells per 100 uL in 96-well plates and grown for 24 hours in DMEM containing 10% fetal calf serum. After 24 hours, 0.15 ug of pGL3 (CAGA) ₁₂ DNA and 1 uL of Fugene (trade name) were transfected. After 24 hours, 2.5 ng / mL myostatin (GDF-8, R & D Systems) and various concentrations of pET32a-prodomain and pMALc2x-prodomain protein were added. Luciferase activity was measured 24 hours after the addition of myostatin and recombinant prodomain protein (Luciferase Assay System, Promega).

Recombinant pMALc2x-prodomain protein was found to decrease myostatin activity at 31 ng / mL, and completely inhibited myostatin activity at 1 ug / mL. Recombinant pET32a-prodomain protein decreased luciferase activity at 1, 2, and 4 ug / mL. This indicates that recombinant pET32a-prodomain protein inhibits myostatin activity but does not inhibit 100% myostatin activity. As a result, the pET32a-prodomain was shown to have myostatin inhibitory activity lower than that of the pMALc2x-prodomain (see FIG. 13).

In vivo analysis was performed for one month using rainbow trout and the weight, protein content and fat content were measured. Growth experiments for pET32a-prodomain and pMALc2x-prodomain proteins were performed with rainbow trout (0.9g, 360 individuals), and each experimental group was adapted to a 50L tank one week before the experiment. Escherichia coli Rogetta-gami2 (DE3) pLyss transformed with pET32a-prodomain and pMALc2x-prodomain were expressed 24 hours at 12.5 ° C using 0.2M IPTG, a protein inducer, and then centrifuged at 4,000 g for 30 minutes. E. coli was crushed using an ultrasonic crusher, and then a supernatant containing a water-soluble recombinant protein was obtained using a centrifuge. The obtained supernatant was used for the rainbow trout growth experiment. Before treating the two recombinant proteins, the cells were cleaned using siphon, and then, the water of the culture tank was adjusted to 1 L, and the supernatant containing the prepared recombinant proteins (pET32a-prodomain and pMALc2x-prodomain) was added. . The concentration of recombinant protein in the treatment group was 0.05 mg and 0.1 mg of the recombinant protein in 1 L of water, respectively. The control was allowed to give a supernatant of untransformed E. coli Rogetta-gami2 (DE3) pLyss to 0.1 mg / L. Treatment time was 120 minutes and was performed twice a week for 4 weeks. And the growth effect was confirmed by weight change, and after the experiment, biochemical analysis of protein and fat content. Experimental results showed the effect of growth depending on the concentration of soluble recombinant myostatin treated. In the experimental group treated with 0.05 mg of pET32a-prodomain protein, body weight increased by 117% compared to the control group after 4 weeks, and the experimental group treated with 0.1 mg increased 128% (p <0.05). However, the protein and fat content (mg / g) did not show a significant difference compared to the control. In the case of pMALc2x-prodomain protein, the experimental group treated with 0.05 mg increased 126% in weight and the 142% increased in 0.1 mg treated group after 4 weeks (p <0.05). In addition, in the case of protein content (mg / g), the experimental group treated with 0.05 mg was increased by 3 mg per g, and the experimental group treated with 0.1 mg 6 mg per g compared to the control (p <0.05). However, although the fat content appeared to be insignificant, the mean value seems to decrease slightly (p> 0.05). pMALc2x-prodomains showed much higher growth effects and higher protein content than pET32a-prodomains. It was shown that pMALc2x-prodomain protein inhibited myostatin activity more than pET32a-prodomain protein in vitro experiments as well as in vitro (see FIG. 14). In Escherichia coli, the water-soluble recombinant pET32a-prodomain and pMALc2x-prodomain proteins showed in vivo and in vitro assays to inhibit the activity of myostatin protein, increasing the growth effect and protein content. Therefore, expression of soluble myostatin prodomains in Escherichia coli Rogetta-gami2 (DE3) pLyss by inserting them into expression vectors pET32a and pMALc2x to promote muscle growth or increase the weight gain of plants and animals, or to reduce atrophy as well as low fat feed additives. It can be used as a pharmaceutical composition for the prevention and treatment of muscle-related diseases such as muscular dystrophy.

TABLE 4

<110> Kangnung-Wonju National University <120> Soluble myostatin drodomain recombination protein having          myostatin inhibitory activity and its use <160> 78 <170> KopatentIn 1.71 <210> 1 <211> 266 <212> PRT <213> myostatin prodomain of bostaurus <400> 1 Met Gln Lys Leu Gln Ile Ser Val Tyr Ile Tyr Leu Phe Met Leu Ile   1 5 10 15 Val Ala Gly Pro Val Asp Leu Asn Glu Asn Ser Glu Gln Lys Glu Asn              20 25 30 Val Glu Lys Glu Gly Leu Cys Asn Ala Cys Leu Trp Arg Glu Asn Thr          35 40 45 Thr Ser Ser Arg Leu Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Thr Ala Pro Asn Ile Ser Lys Asp Ala Ile Arg Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Leu Glu Leu Ile Asp Gln Phe Asp Val                  85 90 95 Gln Arg Asp Ala Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Arg Thr Glu Thr Val Ile Thr Met Pro Thr Glu Ser Asp Leu Leu         115 120 125 Thr Gln Val Glu Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Leu Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Pro Val Lys Thr Pro Ala Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Leu Asp Glu Asn Gly His Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Glu Pro Gly Glu Asp Gly Leu Thr Pro Phe Leu Glu Val Lys                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 2 <211> 267 <212> PRT <213> myostatin prodomain of Norway rat <400> 2 Met Ile Gln Lys Pro Gln Met Tyr Val Tyr Ile Tyr Leu Phe Val Leu   1 5 10 15 Ile Ala Ala Gly Pro Val Asp Leu Asn Glu Asp Ser Glu Arg Glu Ala              20 25 30 Asn Val Glu Lys Glu Gly Leu Cys Asn Ala Cys Ala Trp Arg Gln Asn          35 40 45 Thr Arg Tyr Ser Arg Ile Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys      50 55 60 Leu Arg Leu Glu Thr Ala Pro Asn Ile Ser Lys Asp Ala Ile Arg Gln  65 70 75 80 Leu Leu Pro Arg Ala Pro Pro Leu Arg Glu Leu Ile Asp Gln Tyr Asp                  85 90 95 Val Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr             100 105 110 His Ala Thr Thr Glu Thr Ile Ile Thr Met Pro Thr Glu Ser Asp Phe         115 120 125 Leu Met Gln Ala Asp Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser     130 135 140 Ser Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr 145 150 155 160 Leu Arg Ala Val Lys Thr Pro Thr Thr Val Phe Val Gln Ile Leu Arg                 165 170 175 Leu Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser             180 185 190 Leu Lys Leu Asp Met Ser Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp         195 200 205 Val Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu     210 215 220 Gly Ile Glu Ile Lys Ala Leu Asp Glu Asn Gly His Asp Leu Ala Val 225 230 235 240 Thr Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val                 245 250 255 Lys Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 3 <211> 266 <212> PRT <213> myostatin prodomain of mouse <400> 3 Met Gln Lys Leu Gln Met Tyr Val Tyr Ile Tyr Leu Phe Met Leu Ile   1 5 10 15 Ala Ala Gly Pro Val Asp Leu Asn Glu Gly Ser Glu Arg Glu Glu Asn              20 25 30 Val Glu Lys Glu Gly Leu Cys Asn Ala Cys Ala Trp Arg Gln Asn Thr          35 40 45 Arg Tyr Ser Arg Ile Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Thr Ala Pro Asn Ile Ser Lys Asp Ala Ile Arg Gln Leu  65 70 75 80 Leu Pro Arg Ala Pro Pro Leu Arg Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Thr Thr Glu Thr Ile Ile Thr Met Pro Thr Glu Ser Asp Phe Leu         115 120 125 Met Gln Ala Asp Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Pro Val Lys Thr Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Ser Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Leu Asp Glu Asn Gly His Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val Lys                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 4 <211> 266 <212> PRT <213> myostatin prodomain of pig <400> 4 Met Gln Lys Leu Gln Ile Tyr Val Tyr Ile Tyr Leu Phe Met Leu Ile   1 5 10 15 Val Ala Gly Pro Val Asp Leu Asn Glu Asn Ser Glu Gln Lys Glu Asn              20 25 30 Val Glu Lys Glu Gly Leu Cys Asn Ala Cys Met Trp Arg Gln Asn Thr          35 40 45 Lys Ser Ser Arg Leu Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Thr Ala Pro Asn Ile Ser Lys Asp Ala Ile Arg Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Arg Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Thr Thr Glu Thr Ile Ile Thr Met Pro Thr Glu Ser Asp Leu Leu         115 120 125 Met Gln Val Glu Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Pro Val Lys Thr Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Leu Asp Glu Asn Gly His Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val Lys                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 5 <211> 266 <212> PRT <213> myostatin prodomain of chicken <400> 5 Met Gln Lys Leu Ala Val Tyr Val Tyr Ile Tyr Leu Phe Met Gln Ile   1 5 10 15 Ala Val Asp Pro Val Ala Leu Asp Gly Ser Ser Gln Pro Thr Glu Asn              20 25 30 Ala Glu Lys Asp Gly Leu Cys Asn Ala Cys Thr Trp Arg Gln Asn Thr          35 40 45 Lys Ser Ser Arg Ile Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Gln Ala Pro Asn Ile Ser Arg Asp Val Ile Lys Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Gln Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Thr Thr Glu Thr Ile Ile Thr Met Pro Thr Glu Ser Asp Phe Leu         115 120 125 Val Gln Met Glu Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Gln Val Gln Lys Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Phe Asp Glu Thr Gly Arg Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val Arg                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 6 <211> 266 <212> PRT <213> myostatin prodomain of turkey <400> 6 Met Gln Lys Leu Ala Val Tyr Val Tyr Ile Tyr Leu Phe Met Gln Ile   1 5 10 15 Leu Val His Pro Val Ala Leu Asp Gly Ser Ser Gln Pro Thr Glu Asn              20 25 30 Ala Glu Lys Asp Gly Leu Cys Asn Ala Cys Thr Trp Arg Gln Asn Thr          35 40 45 Lys Ser Ser Arg Ile Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Gln Ala Pro Asn Ile Ser Arg Asp Val Ile Lys Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Gln Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Thr Thr Glu Thr Ile Ile Thr Met Pro Thr Glu Ser Asp Phe Leu         115 120 125 Val Gln Met Glu Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Gln Val Gln Lys Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Phe Asp Glu Asn Gly Arg Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val Arg                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 7 <211> 266 <212> PRT <213> myostatin prodomain of sheep <400> 7 Met Gln Lys Leu Gln Ile Phe Val Tyr Ile Tyr Leu Phe Met Leu Leu   1 5 10 15 Val Ala Gly Pro Val Asp Leu Asn Glu Asn Ser Glu Gln Lys Glu Asn              20 25 30 Val Glu Lys Lys Gly Leu Cys Asn Ala Cys Leu Trp Arg Gln Asn Asn          35 40 45 Lys Ser Ser Arg Leu Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Thr Ala Pro Asn Ile Ser Lys Asp Ala Ile Arg Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Arg Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Val Thr Thr Glu Thr Val Ile Thr Met Pro Thr Glu Ser Asp Leu Leu         115 120 125 Ala Glu Val Gln Glu Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln His Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Pro Val Lys Thr Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Leu Asp Glu Asn Gly His Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Glu Pro Gly Glu Glu Gly Leu Asn Pro Phe Leu Glu Val Lys                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 8 <211> 266 <212> PRT <213> myostatin prodomain of human <400> 8 Met Gln Lys Leu Gln Leu Cys Val Tyr Ile Tyr Leu Phe Met Leu Ile   1 5 10 15 Val Ala Gly Pro Val Asp Leu Asn Glu Asn Ser Glu Gln Lys Glu Asn              20 25 30 Val Glu Lys Glu Gly Leu Cys Asn Ala Cys Thr Trp Arg Gln Asn Thr          35 40 45 Lys Ser Ser Arg Ile Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Thr Ala Pro Asn Ile Ser Lys Asp Val Ile Arg Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Arg Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Thr Thr Glu Thr Ile Ile Thr Met Pro Thr Glu Ser Asp Phe Leu         115 120 125 Met Gln Val Asp Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Pro Val Glu Thr Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Leu Asp Glu Asn Gly His Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val Lys                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 9 <211> 266 <212> PRT <213> myostatin prodomain of dog <400> 9 Met Gln Arg Leu Gln Ile Cys Val Tyr Ile Tyr Leu Phe Val Leu Ile   1 5 10 15 Val Ala Gly Pro Val Asp Leu Ser Glu Asn Ser Glu Gln Lys Glu Asn              20 25 30 Val Glu Lys Glu Gly Leu Cys Asn Ala Cys Met Trp Arg Gln Asn Thr          35 40 45 Lys Ser Ser Arg Ile Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Thr Ala Pro Asn Ile Ser Arg Asp Ala Val Arg Gln Leu  65 70 75 80 Leu Pro Arg Ala Pro Pro Leu Arg Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Thr Thr Glu Thr Val Ile Ala Met Pro Ala Glu Thr Asp Leu Leu         115 120 125 Met Gln Val Glu Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Pro Val Lys Thr Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Leu Asp Glu Asn Gly His Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val Lys                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 10 <211> 266 <212> PRT Myostatin prodomain of anasplatyrhynchos (mallard) <400> 10 Met Gln Lys Leu Ala Val Tyr Val Tyr Ile Tyr Leu Phe Met Leu Ile   1 5 10 15 Ser Val Asp Pro Val Ala Leu Asp Asp Gly Ser Gln Pro Thr Glu Asn              20 25 30 Ala Glu Lys Asp Gly Leu Cys Asn Ala Cys Thr Trp Arg Gln Asn Thr          35 40 45 Lys Ser Ser Arg Ile Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Gln Ala Pro Asn Ile Ser Arg Asp Val Ile Lys Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Gln Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Thr Thr Glu Thr Ile Ile Thr Met Pro Thr Glu Ser Asp Phe Leu         115 120 125 Val Gln Met Glu Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Gln Val Gln Lys Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Pro Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Phe Asp Glu Asn Gly Arg Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val Arg                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 11 <211> 266 <212> PRT <213> myostatin prodomain of coturnix coturnix <400> 11 Met Gln Lys Ile Val Val Tyr Val Tyr Ile Tyr Leu Phe Val Gln Ile   1 5 10 15 Ser Val Asp Pro Val Ala Leu Asp Gly Ser Ser Gln Pro Thr Glu Asn              20 25 30 Thr Glu Lys Asp Gly Leu Cys Asn Ala Cys Thr Trp Arg Gln Asn Thr          35 40 45 Lys Ser Ser Arg Ile Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Gln Ala Pro Asn Ile Ser Arg Asp Val Ile Lys Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Gln Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Thr Thr Glu Thr Ile Ile Thr Met Pro Thr Glu Ser Asp Phe Leu         115 120 125 Val Gln Met Glu Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Gln Val Gln Lys Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Asn Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Phe Asp Glu Asn Gly Arg Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val Arg                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 12 <211> 266 <212> PRT <213> myostatin prodomain of macacafascicularis (cynomolgusmonkey) <400> 12 Met Gln Lys Leu Gln Leu Cys Val Tyr Ile Tyr Leu Phe Met Leu Ile   1 5 10 15 Val Ala Gly Pro Val Asp Leu Asn Glu Asn Ser Glu Gln Lys Glu Asn              20 25 30 Val Glu Lys Glu Gly Leu Cys Asn Ala Cys Thr Trp Arg Gln Asn Thr          35 40 45 Lys Ser Ser Arg Ile Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Thr Ala Pro Asn Ile Ser Lys Asp Ala Ile Arg Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Arg Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Thr Thr Glu Thr Ile Ile Thr Met Pro Thr Glu Ser Asp Phe Leu         115 120 125 Met Gln Val Asp Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Pro Val Glu Thr Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Leu Asp Glu Asn Gly His Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val Lys                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 13 <211> 266 <212> PRT <213> myostatin prodomain of gorilla gorilla gorilla (lowlandgorilla) <400> 13 Met Gln Lys Leu Gln Leu Cys Val Tyr Ile Tyr Leu Phe Met Leu Ile   1 5 10 15 Val Ala Gly Pro Val Asp Leu Asn Glu Asn Ser Glu Gln Lys Glu Asn              20 25 30 Val Glu Lys Glu Gly Leu Cys Asn Ala Cys Thr Trp Arg Gln Asn Thr          35 40 45 Lys Ser Ser Arg Ile Glu Ala Ile Lys Ile Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Glu Thr Ala Pro Asn Ile Ser Lys Asp Ala Ile Arg Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Arg Glu Leu Ile Asp Gln Tyr Asp Val                  85 90 95 Gln Arg Asp Asp Ser Ser Asp Gly Ser Leu Glu Asp Asp Asp Tyr His             100 105 110 Ala Thr Thr Glu Thr Ile Ile Thr Met Pro Thr Glu Ser Asp Phe Leu         115 120 125 Met Gln Val Asp Gly Lys Pro Lys Cys Cys Phe Phe Lys Phe Ser Ser     130 135 140 Lys Ile Gln Tyr Asn Lys Val Val Lys Ala Gln Leu Trp Ile Tyr Leu 145 150 155 160 Arg Pro Val Glu Thr Pro Thr Thr Val Phe Val Gln Ile Leu Arg Leu                 165 170 175 Ile Lys Pro Met Lys Asp Gly Thr Arg Tyr Thr Gly Ile Arg Ser Leu             180 185 190 Lys Leu Asp Met Asn Pro Gly Thr Gly Ile Trp Gln Ser Ile Asp Val         195 200 205 Lys Thr Val Leu Gln Asn Trp Leu Lys Gln Pro Glu Ser Asn Leu Gly     210 215 220 Ile Glu Ile Lys Ala Leu Asp Glu Asn Gly His Asp Leu Ala Val Thr 225 230 235 240 Phe Pro Gly Pro Gly Glu Asp Gly Leu Asn Pro Phe Leu Glu Val Lys                 245 250 255 Val Thr Asp Thr Pro Lys Arg Ser Arg Arg             260 265 <210> 14 <211> 268 <212> PRT <213> myostatin prodomain of haliotis discus hannai <400> 14 Met His Leu Ser His Ile Val Leu Tyr Leu Ser Leu Leu Val Ala Leu   1 5 10 15 Gly Pro Val Val Leu Ser Asp Gln Glu Thr His His Gln Gln Pro Ser              20 25 30 Ala Ser Ser Pro Glu Asp Ala Glu Gln Cys Ala Thr Cys Asp Val Arg          35 40 45 Gln Gln Ile Lys Thr Met Arg Leu Asn Ala Ile Lys Ser Gln Ile Leu      50 55 60 Ser Lys Leu Arg Met Lys Glu Ala Pro Asn Ile Ser Arg Asp Ile Val  65 70 75 80 Lys Gln Leu Leu Pro Lys Ala Pro Pro Leu Gln Gln Leu Leu Asp Gln                  85 90 95 Tyr Asp Val Leu Gly Asp Asp Asn Arg Asp Val Val Met Glu Asp Asp             100 105 110 Asp Glu His Ala Thr Thr Glu Thr Ile Met Met Ala Thr Glu Pro         115 120 125 Glu Pro Ile Val Gln Val Asp Ala Glu Pro Lys Cys Cys Phe Phe Ser     130 135 140 Phe Thr Gln Lys Phe Gln Ala Ser Arg Ile Val Arg Ala Gln Leu Trp 145 150 155 160 Val Tyr Leu Arg Pro Ala Asp Glu Ala Thr Thr Val Phe Leu Gln Ile                 165 170 175 Ser Arg Leu Met Pro Val Thr Asp Gly Ser Arg His Ile Arg Ile Arg             180 185 190 Ser Leu Lys Ile Asp Val Asn Ala Gly Leu Ser Ser Trp Gln Ser Ile         195 200 205 Asp Val Lys Gln Val Leu Thr Val Trp Leu Arg Gln Pro Glu Thr Asn     210 215 220 Trp Gly Ile Glu Ile Asn Ala Phe Asp Ser Arg Gly Asn Asp Leu Ala 225 230 235 240 Val Thr Ser Thr Glu Pro Gly Glu Glu Gly Leu Gln Pro Phe Met Glu                 245 250 255 Val Lys Ile Thr Asp Gly Pro Lys Arg Val Arg Arg             260 265 <210> 15 <211> 268 <212> PRT <213> myostatin prodomain of kareiusbicoloratus (stone flounder) <400> 15 Met His Pro Ser His Phe Val Leu Tyr Leu Thr Leu Leu Val Ala Val   1 5 10 15 Gly Pro Val Val Leu Ser Glu Gln Glu Thr His His Gln Gln Pro Ser              20 25 30 Ala Ser Ser Pro Glu Asp Ala Glu Pro Cys Ala Thr Cys Glu Val Arg          35 40 45 Gln Gln Ile Lys Thr Met Arg Leu Asn Ala Ile Lys Ser Gln Ile Leu      50 55 60 Ser Lys Leu Arg Met Lys Glu Ala Pro Asn Ile Ser Arg Asp Val Val  65 70 75 80 Lys Gln Leu Leu Pro Lys Ala Pro Pro Leu Gln Gln Leu Leu Asp Gln                  85 90 95 Tyr Asp Val Leu Gly Asp Asp Asn Arg Val Val Val Glu Glu Asp             100 105 110 Asp Glu His Ala Ile Thr Glu Thr Ile Met Met Ala Val Glu Pro         115 120 125 Glu Pro Ile Val Gln Val Asp Ala Glu Pro Lys Cys Cys Phe Phe Ser     130 135 140 Phe Thr Gln Lys Phe Gln Ala Ser Arg Val Val Arg Ala Gln Leu Trp 145 150 155 160 Val His Leu Arg Pro Ala Val Glu Ala Ser Thr Val Phe Leu Gln Ile                 165 170 175 Ser Arg Leu Met Pro Val Thr Asp Gly Ser Arg His Ile Arg Ile Arg             180 185 190 Ser Leu Lys Ile Asp Val Asn Ala Gly Val Ser Ser Trp Gln Ser Ile         195 200 205 Asp Val Lys Gln Val Leu Ser Val Trp Leu Arg Gln Pro Glu Thr Asn     210 215 220 Trp Gly Ile Glu Ile Asn Ala Phe Asp Ser Arg Gly Asn Asp Leu Ala 225 230 235 240 Val Thr Ser Thr Glu Pro Gly Glu Glu Gly Leu Gln Pro Phe Met Glu                 245 250 255 Val Lys Ile Ser Glu Gly Pro Arg Arg Val Arg Arg             260 265 <210> 16 <211> 267 <212> PRT <213> myostatin prodomain of psetta maxima (turbot) <400> 16 Met His Gln Ser Gln Ile Val Leu Tyr Leu Ser Leu Leu Ile Ala Leu   1 5 10 15 Gly Ala Val Val Leu Ser Asp Gln Glu Thr Tyr Gln Gln Pro Ser Ala              20 25 30 Thr Ser Pro Glu Asp Ala Glu Gln Cys Ala Thr Cys Glu Val Arg Gln          35 40 45 Gln Ile Lys Thr Met Arg Leu Asn Ala Ile Lys Ser Gln Ile Leu Ser      50 55 60 Lys Leu Arg Met Lys Glu Ala Pro Asn Ile Ser Arg Asp Ile Val Lys  65 70 75 80 Gln Leu Leu Pro Lys Ala Pro Pro Leu Gln Gln Leu Leu Asp Gln Tyr                  85 90 95 Asp Val Leu Gly Asp Asp Asn Arg Asp Val Val Met Glu Asp Asp Asp             100 105 110 Glu His Ala Thr Thr Glu Thr Ile Met Met Ala Thr Glu Pro Glu         115 120 125 Ser Ile Val Gln Val Asp Asp Glu Pro Lys Cys Cys Phe Phe Ser Phe     130 135 140 Thr Gln Lys Phe Gln Ala Ser Arg Ile Val Arg Ala Gln Leu Trp Val 145 150 155 160 His Leu Arg Pro Ala Val Glu Ala Thr Thr Val Phe Leu Gln Ile Ala                 165 170 175 Arg Leu Met Pro Val Thr Glu Gly Asn Arg His Ile Arg Ile Arg Ser             180 185 190 Leu Lys Ile Asp Val Asn Ala Gly Thr Ser Ser Trp Gln Ser Ile Asp         195 200 205 Val Lys Gln Val Leu Ala Val Trp Leu Arg Gln Pro Glu Thr Asn Trp     210 215 220 Gly Ile Glu Ile Asn Ala Phe Asp Ser Arg Gly Asn Asp Leu Ala Val 225 230 235 240 Thr Ser Pro Glu Pro Gly Glu Glu Gly Leu Gln Pro Phe Met Glu Val                 245 250 255 Lys Ile Ser Glu Asn Pro Lys Arg Ala Arg Arg             260 265 <210> 17 <211> 268 <212> PRT <213> myostatin prodomain of sebastes schlegelii <400> 17 Met His Leu Ser His Ile Val Leu Tyr Leu Ser Leu Leu Val Ala Leu   1 5 10 15 Gly Pro Val Val Leu Ser Asp Gln Glu Thr His Gln Gln Pro Pro Ser              20 25 30 Ala Ala Ser Pro Gly Glu Thr Glu Gln Cys Ala Thr Cys Glu Val Arg          35 40 45 Gln Gln Ile Lys Thr Met Arg Leu Asn Ala Ile Lys Ser Gln Ile Leu      50 55 60 Ser Lys Leu Arg Met Lys Glu Ala Pro Asn Ile Ser Arg Asp Ile Val  65 70 75 80 Lys Gln Leu Leu Pro Lys Ala Pro Pro Leu Gln Gln Leu Leu Asp Gln                  85 90 95 Tyr Asp Val Leu Gly Asp Asp Asn Lys Asp Val Val Met Glu Glu Asp             100 105 110 Asp Glu His Ala Thr Thr Glu Thr Val Met Met Met Ala Thr Glu Pro         115 120 125 Ala Ser Ile Val Gln Val Ala Glu Glu Pro Lys Cys Cys Phe Phe Ser     130 135 140 Phe Ser Pro Lys Phe Gln Ala Ser Arg Ile Val Arg Ala Gln Leu Trp 145 150 155 160 Val His Leu Arg Pro Ala Thr Glu Ala Thr Thr Val Phe Leu Gln Ile                 165 170 175 Ser Arg Leu Met Pro Val Thr Asp Gly Ser Arg His Ile Arg Ile Arg             180 185 190 Ser Leu Lys Ile Asp Val Asn Ala Gly Leu Ser Ser Trp Gln Ser Ile         195 200 205 Asp Val Lys Gln Val Leu Thr Val Trp Leu Arg Gln Pro Glu Thr Asn     210 215 220 Trp Gly Ile Glu Ile Asn Ala Phe Asp Ser Arg Gly Asn Asp Leu Ala 225 230 235 240 Val Thr Ser Thr Glu Pro Gly Glu Glu Gly Leu Gln Pro Phe Met Glu                 245 250 255 Val Lys Val Ser Glu Gly Pro Arg Arg Ala Arg Arg             260 265 <210> 18 <211> 267 <212> PRT <213> myostatin prodomain of oreochromis mossambicus (mozambiquetilapia) <400> 18 Met His Leu Ser Gln Ile Val Leu Tyr Leu Ser Leu Leu Ile Ala Leu   1 5 10 15 Gly Pro Val Val Leu Ser Asp Gln Glu Ala His Gln Gln Pro Ser Val              20 25 30 Ser Thr Pro Val Asp Thr Asp Gln Cys Ala Thr Cys Glu Val Arg Gln          35 40 45 Gln Ile Lys Thr Met Arg Leu Asn Ala Ile Lys Ser Gln Ile Leu Ser      50 55 60 Lys Leu Arg Met Lys Glu Ala Pro Asn Ile Ser Arg Glu Ile Val Lys  65 70 75 80 Gln Leu Leu Pro Lys Ala Pro Pro Leu Gln Gln Leu Leu Asp Gln Tyr                  85 90 95 Asp Val Leu Gly Asp Asp Asn Arg Glu Glu Val Leu Glu Asp Asp Asp             100 105 110 Glu His Ala Thr Thr Glu Thr Ile Val Met Val Ala Thr Glu Pro Asp         115 120 125 Pro Ala Val Gln Val Asp Gly Gln Pro Lys Cys Cys Phe Phe Ser Ile     130 135 140 Thr Gln Lys Phe Gln Ala Ser Arg Val Val Arg Ala Gln Leu Trp Val 145 150 155 160 His Leu Arg Pro Ser Glu Glu Val Thr Thr Val Phe Leu Gln Ile Ser                 165 170 175 Arg Leu Ile Pro Val Thr Asp Gly Asn Arg His Ile Arg Ser Arg Ser             180 185 190 Leu Lys Ile Asp Val Asn Pro Gly Pro Ala Ser Trp Gln Ser Ile Asp         195 200 205 Val Lys Gln Val Leu Thr Val Trp Leu Arg Gln Pro Glu Thr Asn Trp     210 215 220 Gly Ile Glu Ile Asn Ala Phe Asp Ser Arg Gly Asn Asp Leu Ala Val 225 230 235 240 Thr Ser Ala Glu Pro Gly Glu Glu Gly Leu Gln Pro Phe Met Glu Val                 245 250 255 Lys Ile Ser Glu Gly Pro Arg Arg Ala Arg Arg             260 265 <210> 19 <211> 275 <212> PRT <213> myostatin prodomain of pagrus major (redseabream) <400> 19 Met His Pro Ser Gln Ile Val Leu Tyr Leu Ser Leu Leu Ile Val Leu   1 5 10 15 Gly Pro Val Val Leu Ser Asp Gln Glu Thr Gln Gln Gln Gln Gln Gln              20 25 30 Gln Gln Gln Gln Gln Pro Ser Ala Thr Gly Pro Glu Asp Thr Glu Gln          35 40 45 Cys Ala Thr Cys Glu Val Arg Gln Gln Ile Lys Thr Met Arg Leu Asn      50 55 60 Ala Ile Lys Ser Gln Ile Leu Ser Lys Leu Arg Met Lys Glu Ala Pro  65 70 75 80 Asn Ile Ser Arg Asp Ile Val Lys Gln Leu Leu Pro Lys Ala Pro Pro                  85 90 95 Leu Gln Gln Leu Leu Asp Gln Tyr Asp Val Leu Gly Asp Asp Asn Arg             100 105 110 Asp Val Val Met Glu Glu Asp Asp Glu His Ala Ile Thr Glu Thr Ile         115 120 125 Met Met Met Ala Thr Glu Pro Glu Ser Val Val Gln Val Asp Gly Glu     130 135 140 Pro Arg Cys Cys Phe Phe Ser Phe Thr Gln Lys Ile Gln Ala Asn Arg 145 150 155 160 Ile Val Arg Ala Gln Leu Trp Val His Leu Arg Ala Ser Asp Glu Ala                 165 170 175 Thr Thr Val Phe Leu Gln Ile Ser Arg Leu Met Pro Val Thr Asp Gly             180 185 190 Asn Gly His Ile His Ile Arg Ser Leu Lys Ile Asp Val Asn Ala Gly         195 200 205 Val Gly Ser Trp Gln Ser Ile Asp Val Lys Gln Val Leu Ser Val Trp     210 215 220 Leu Arg Gln Pro Glu Thr Asn Trp Gly Ile Gln Ile Asn Ala Phe Asp 225 230 235 240 Ser Arg Gly Asn Asp Leu Ala Val Thr Ser Ala Glu Pro Gly Glu Asp                 245 250 255 Gly Leu Gln Pro Phe Met Glu Val Lys Ile Ser Glu Gly Pro Lys Arg             260 265 270 Val arg arg         275 <210> 20 <211> 273 <212> PRT <213> myostatin prodomain of acanthopagrus schlegelii (blackseabream) <400> 20 Met His Pro Ser Gln Ile Val Leu Tyr Leu Ser Leu Leu Ile Val Leu   1 5 10 15 Gly Pro Val Val Leu Ser Glu Gln Glu Thr Gln Gln Gln Gln Gln Gln              20 25 30 Gln Gln Gln Pro Ser Ala Thr Ser Pro Glu Asp Thr Glu Leu Cys Ala          35 40 45 Thr Cys Glu Val Arg Gln Gln Ile Lys Thr Met Arg Leu Asn Ala Ile      50 55 60 Lys Ser Gln Ile Leu Ser Lys Leu Arg Met Lys Glu Ala Pro Asn Ile  65 70 75 80 Ser Arg Asp Ile Val Lys Gln Leu Leu Pro Lys Ala Pro Pro Leu Gln                  85 90 95 Gln Leu Leu Asp Gln Tyr Asp Val Leu Gly Asp Asp Asn Arg Asp Ala             100 105 110 Val Met Glu Asp Asp Asp Glu His Ala Ile Thr Glu Thr Ile Met Met         115 120 125 Met Ala Thr Glu Pro Glu Pro Val Val Gln Val Asp Gly Glu Pro Arg     130 135 140 Cys Cys Phe Phe Ser Phe Thr Gln Lys Ile Gln Asp Asn Arg Ile Val 145 150 155 160 Arg Ala Gln Leu Trp Val His Leu Arg Ala Ser Asp Glu Ala Thr Thr                 165 170 175 Val Phe Leu Gln Ile Ser Arg Leu Met Pro Val Thr Asp Gly Asn Gly             180 185 190 His Ile His Ile Arg Ser Leu Lys Ile Asp Val Asn Ala Gly Val Gly         195 200 205 Ser Trp Gln Ser Ile Asp Val Lys Gln Val Leu Ser Val Trp Leu Arg     210 215 220 Gln Pro Glu Thr Asn Trp Gly Ile Gln Ile Asn Ala Phe Asp Ser Arg 225 230 235 240 Gly Asn Asp Leu Ala Val Thr Ser Ala Glu Pro Gly Glu Asp Gly Leu                 245 250 255 Gln Pro Phe Met Glu Val Lys Ile Ser Glu Gly Pro Arg Arg Val Arg             260 265 270 Arg     <210> 21 <211> 267 <212> PRT <213> myostatin prodomain of takifugu rubripes (fugurubripes) <400> 21 Met Gln Leu Ser Pro Ser Met Leu His Phe Ser Leu Met Ile Ser Leu   1 5 10 15 Ser Leu Val Val Leu Ser Gly Gln Glu Thr His Gln Gln Pro Pro Val              20 25 30 Gly Ser Pro Glu Asp Thr Glu Gln Cys Val Thr Cys Asp Val Arg Gln          35 40 45 His Ile Lys Thr Met Arg Leu Asn Ala Ile Lys Pro Gln Ile Leu Ser      50 55 60 Lys Leu Arg Met Lys Glu Ala Pro Asn Ile Ser Arg Asp Thr Val Lys  65 70 75 80 Gln Leu Leu Pro Lys Ala Pro Pro Leu Gln Gln Leu Leu Asp Gln Tyr                  85 90 95 Asp Val Leu Gly Asp Asp Asn Arg Asp Val Val Thr Glu Glu Asp Asp             100 105 110 Glu His Ala Ile Thr Glu Thr Ile Met Met Met Ala Thr Glu Pro Ala         115 120 125 Ser Val Val Gln Val Asn Gly Glu Pro Lys Cys Cys His Phe Ser Phe     130 135 140 Thr Gln Lys Phe Gln Val Ser Arg Leu Val Arg Ala Gln Leu Trp Val 145 150 155 160 His Leu Arg Pro Ala Ala Glu Ala Thr Thr Val Phe Leu Gln Ile Ser                 165 170 175 Arg Leu Met Pro Val Thr Asp Gly Asn Arg His Ile Arg Ile Arg Ser             180 185 190 Leu Lys Leu Asp Val Lys Ala Gly Val Ser Ser Trp Gln Ser Ile Asp         195 200 205 Val Lys Gln Val Leu Ser Val Trp Leu Arg Gln Pro Glu Thr Asn Trp     210 215 220 Gly Ile Glu Ile Asn Ala Phe Asp Ser Arg Gly Lys Asp Leu Ala Val 225 230 235 240 Thr Ser Thr Gln Pro Gly Glu Glu Gly Leu Gln Pro Phe Met Glu Val                 245 250 255 Lys Ile Ser Glu Gly Pro Arg Arg Val Arg Arg             260 265 <210> 22 <211> 264 <212> PRT <213> myostatin prodomain of oncorhynchus mykiss (rainbowtrout) <400> 22 Met His Leu Thr Gln Val Leu Ile Tyr Leu Ser Phe Met Val Ala Phe   1 5 10 15 Gly Pro Val Gly Leu Gly Asp Gln Thr Ala His His Gln Pro Pro Ala              20 25 30 Thr Asp Asp Gly Glu Gln Cys Ser Thr Cys Glu Val Arg Gln Gln Ile          35 40 45 Lys Asn Met Arg Leu His Ala Ile Lys Ser Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Lys Gln Ala Pro Asn Ile Ser Arg Asp Val Val Lys Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Gln Gln Leu Leu Asp Gln Tyr Asp Val                  85 90 95 Leu Gly Asp Asp Asn Lys Asp Gly Leu Met Glu Glu Asp Asp Glu His             100 105 110 Ala Ile Thr Glu Thr Ile Met Thr Met Ala Thr Glu Pro Glu Ser Ile         115 120 125 Val Gln Val Asp Arg Lys Pro Lys Cys Cys Leu Phe Ser Phe Ser Ser     130 135 140 Lys Ile Gln Val Asn Arg Ile Val His Ala Gln Leu Trp Val His Leu 145 150 155 160 Leu Pro Ala Asp Glu Val Thr Thr Val Phe Leu Gln Ile Ser Arg Leu                 165 170 175 Met Pro Val Thr Asp Gly Gly Arg His Ile Gly Ile Arg Ser Leu Lys             180 185 190 Ile Asp Val Asn Ala Gly Val Ser Ser Trp Gln Ser Ile Asp Val Lys         195 200 205 Gln Val Leu Ser Val Trp Leu Arg Gln Pro Glu Thr Asn Trp Gly Ile     210 215 220 Glu Ile Asn Ala Phe Asp Ser Lys Gly Asn Asp Leu Ala Val Thr Ser 225 230 235 240 Ala Glu Ala Gly Glu Gly Leu Gln Pro Phe Met Glu Val Thr Ile Ser                 245 250 255 Glu Gly Pro Lys Arg Phe Arg Arg             260 <210> 23 <211> 264 <212> PRT <213> myostatin prodomain of salmo salar (atalantic salmon) myostatin 1a <400> 23 Met His Leu Thr Gln Val Leu Ile Tyr Leu Ser Phe Met Val Ala Phe   1 5 10 15 Gly Pro Val Gly Leu Gly Asp Gln Thr Ala His His Gln Pro Pro Ala              20 25 30 Thr Asp Asp Gly Glu Gln Cys Pro Thr Cys Glu Val Arg Gln Gln Ile          35 40 45 Lys Asn Met Arg Leu His Ala Ile Lys Ser Gln Ile Leu Ser Lys Leu      50 55 60 Arg Leu Lys Gln Ala Pro Asn Ile Ser Arg Asp Val Val Lys Gln Leu  65 70 75 80 Leu Pro Lys Ala Pro Pro Leu Gln Gln Leu Leu Asp Gln Tyr Asp Val                  85 90 95 Leu Gly Asp Asp Asn Lys Asp Gly Val Met Glu Glu Asp Asp Glu His             100 105 110 Ala Ile Thr Glu Thr Ile Met Thr Met Ala Thr Glu Pro Gln Ser Ile         115 120 125 Val Gln Val Asp Arg Lys Pro Lys Cys Cys Leu Phe Ser Phe Ser Ser     130 135 140 Lys Ile Gln Val Asn Arg Ile Val His Ala Gln Leu Trp Val His Leu 145 150 155 160 Leu Pro Ala Asp Glu Val Thr Thr Val Phe Leu Gln Ile Ser Arg Leu                 165 170 175 Met Pro Val Thr Asp Gly Gly Arg His Ile Gly Ile Arg Ser Leu Lys             180 185 190 Ile Asp Val Asn Ala Gly Val Ser Ser Trp Gln Ser Ile Asp Val Lys         195 200 205 Gln Val Leu Ser Val Trp Leu Arg Gln Pro Glu Thr Asn Trp Gly Ile     210 215 220 Glu Ile Asn Ala Phe Asp Ser Lys Gly Asn Asp Leu Ala Val Thr Ser 225 230 235 240 Ala Glu Ala Gly Glu Gly Leu Gln Pro Phe Met Glu Val Thr Ile Ser                 245 250 255 Glu Gly Pro Lys Arg Ser Arg Arg             260 <210> 24 <211> 266 <212> PRT <213> myostatin prodomain of pimephales promelas <400> 24 Met His Phe Thr Gln Val Leu Ile Ser Leu Ser Val Leu Ile Ala Cys   1 5 10 15 Gly Pro Val Gly Asn Gly Asp Ile Thr Ala Pro Gln Gln Pro Ser Thr              20 25 30 Ala Thr Glu Glu Ser Glu Gln Cys Ser Thr Cys Glu Phe Arg Gln His          35 40 45 Ser Lys Leu Met Arg Leu His Ala Ile Lys Ser Gln Ile Leu Ser Lys      50 55 60 Leu Arg Leu Lys Gln Ala Pro Asn Ile Ser Arg Asp Val Val Lys Gln  65 70 75 80 Leu Leu Pro Lys Ala Pro Pro Leu Gln Glu Leu Leu Asp Gln Tyr Asp                  85 90 95 Val Leu Gly Asp Asp Ser Lys Asp Gly Ala Met Glu Glu Asp Asp Glu             100 105 110 His Ala Thr Thr Glu Thr Ile Met Thr Met Ala Thr Glu Pro Asp Pro         115 120 125 Ile Val Gln Val Asp Arg Lys Pro Lys Cys Cys Phe Phe Ser Phe Ser     130 135 140 Pro Lys Ile Gln Ala Asn Arg Ile Val Arg Ala Gln Val Trp Val His 145 150 155 160 Leu Arg Pro Ala Asp Glu Val Thr Thr Val Phe Leu Gln Ile Ser Arg                 165 170 175 Leu Met Pro Val Thr Asp Gly Gly Arg His Ile Arg Ile Arg Ser Leu             180 185 190 Lys Ile Asp Val Asn Ala Gly Val Thr Ser Trp Gln Ser Ile Asp Val         195 200 205 Lys Gln Val Leu Thr Val Trp Leu Arg Gln Pro Glu Thr Asn Trp Gly     210 215 220 Ile Glu Ile Asn Ala Tyr Asp Ala Lys Gly Asn Asp Leu Ala Val Thr 225 230 235 240 Ser Ala Glu Ala Gly Glu Asp Gly Leu Leu Pro Phe Met Glu Val Lys                 245 250 255 Ile Ser Glu Gly Pro Lys Arg Ile Arg Arg             260 265 <210> 25 <211> 268 <212> PRT <213> myostatin prodomain of Rotifer <400> 25 Met His Leu Ser His Ile Val Leu Tyr Leu Ser Leu Leu Val Ala Leu   1 5 10 15 Gly Pro Val Val Leu Ser Asp Gln Glu Thr His His Gln Gln Pro Ser              20 25 30 Ala Ser Ser Pro Glu Asp Ala Glu Gln Cys Ala Thr Cys Asp Val Arg          35 40 45 Gln Gln Ile Lys Thr Met Arg Leu Asn Ala Ile Lys Ser Gln Ile Leu      50 55 60 Ser Lys Leu Arg Met Lys Glu Ala Pro Asn Ile Ser Arg Asp Ile Val  65 70 75 80 Lys Gln Leu Leu Pro Lys Ala Pro Pro Leu Gln Gln Leu Leu Asp Gln                  85 90 95 Tyr Asp Val Leu Gly Asp Asp Asn Arg Asp Val Val Met Glu Asp Asp             100 105 110 Asp Glu His Ala Thr Thr Glu Thr Ile Met Met Ala Thr Glu Pro         115 120 125 Glu Pro Ile Val Gln Val Asp Ala Glu Pro Lys Cys Cys Phe Phe Ser     130 135 140 Phe Thr Gln Lys Phe Gln Ala Ser Arg Ile Val Arg Ala Gln Leu Trp 145 150 155 160 Val Tyr Leu Arg Pro Ala Asp Glu Ala Thr Thr Val Phe Leu Gln Ile                 165 170 175 Ser Arg Leu Met Pro Val Thr Asp Gly Ser Arg His Ile Arg Ile Arg             180 185 190 Ser Leu Lys Ile Asp Val Asn Ala Gly Leu Ser Ser Trp Gln Ser Ile         195 200 205 Asp Val Lys Gln Val Leu Thr Val Trp Leu Arg Gln Pro Glu Thr Asn     210 215 220 Trp Gly Ile Glu Ile Asn Ala Phe Asp Ser Arg Gly Asn Asp Leu Ala 225 230 235 240 Val Thr Ser Thr Glu Pro Gly Glu Glu Gly Leu Gln Pro Phe Met Glu                 245 250 255 Val Lys Ile Thr Asp Gly Pro Lys Arg Val Arg Arg             260 265 <210> 26 <211> 268 <212> PRT <213> myostatin prodomain of flatfish <400> 26 Met His Leu Ser His Ile Val Leu Tyr Leu Ser Leu Leu Val Ala Leu   1 5 10 15 Gly Pro Val Val Leu Ser Asp Gln Glu Thr His His Gln Gln Pro Ser              20 25 30 Ala Ser Ser Pro Glu Asp Ala Glu Gln Cys Ala Thr Cys Asp Val Arg          35 40 45 Gln Gln Ile Lys Thr Met Arg Leu Asn Ala Ile Ile Ser Gln Ile Leu      50 55 60 Ser Lys Leu Arg Met Lys Glu Ala Pro Asn Ile Ser Arg Asp Ile Val  65 70 75 80 Lys Gln Leu Leu Pro Lys Ala Pro Pro Leu Gln Gln Leu Leu Asp Gln                  85 90 95 Tyr Asp Val Leu Gly Asp Asp Asn Arg Asp Val Val Met Glu Asp Asp             100 105 110 Asp Glu His Ala Thr Thr Glu Thr Ile Met Met Ala Thr Glu Pro         115 120 125 Glu Pro Ile Val Gln Val Asp Ala Glu Pro Lys Cys Cys Phe Phe Ser     130 135 140 Phe Thr Gln Lys Phe Gln Ala Ser Arg Ile Val Arg Ala Gln Leu Trp 145 150 155 160 Val Tyr Leu Arg Pro Ala Asp Glu Ala Thr Thr Val Phe Leu Gln Ile                 165 170 175 Ser Arg Leu Met Pro Val Thr Asp Gly Ser Arg His Ile Arg Ile Arg             180 185 190 Ser Leu Lys Ile Asp Val Asn Ala Gly Leu Ser Ser Trp Gln Ser Ile         195 200 205 Asp Val Lys Gln Val Leu Thr Val Trp Leu Arg Gln Pro Glu Thr Asn     210 215 220 Trp Gly Ile Glu Ile Asn Ala Phe Asp Ser Arg Gly Asn Asp Leu Ala 225 230 235 240 Val Thr Ser Thr Glu Pro Gly Glu Glu Gly Leu Gln Pro Phe Met Glu                 245 250 255 Val Lys Ile Thr Asp Gly Pro Lys Arg Val Arg Arg             260 265 <210> 27 <211> 391 <212> PRT <213> pMALc2x-prodomain of bostaurus <400> 27 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 28 <211> 391 <212> PRT <213> pMALc2x-prodomain of Norway rat <400> 28 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 29 <211> 391 <212> PRT <213> pMALc2x-prodomain of mouse <400> 29 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 30 <211> 391 <212> PRT <213> pMALc2x-prodomain of pig <400> 30 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 31 <211> 391 <212> PRT <213> pMALc2x-prodomain of chicken <400> 31 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 32 <211> 391 <212> PRT <213> pMALc2x-prodomain of turkey <400> 32 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 33 <211> 391 <212> PRT <213> pMALc2x-prodomain of sheep <400> 33 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 34 <211> 391 <212> PRT <213> pMALc2x-prodomain of human <400> 34 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 35 <211> 391 <212> PRT <213> pMALc2x-prodomain of dog <400> 35 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 36 <211> 391 <212> PRT <213> pMALc2x-prodomain of anasplatyrhynchos (mallard) <400> 36 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 37 <211> 391 <212> PRT <213> pMALc2x-prodomain of coturnix coturnix <400> 37 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 38 <211> 391 <212> PRT <213> pMALc2x-prodomain of macacafascicularis (cynomolgusmonkey) <400> 38 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 39 <211> 391 <212> PRT <213> pMALc2x-prodomain of gorilla gorilla gorilla (lowlandgorilla) <400> 39 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 40 <211> 391 <212> PRT <213> pMALc2x-prodomain of haliotis discus hannai <400> 40 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 41 <211> 391 <212> PRT <213> pMALc2x-prodomain of kareiusbicoloratus (stone flounder) <400> 41 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 42 <211> 391 <212> PRT <213> pMALc2x-prodomain of psetta maxima (turbot) <400> 42 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 43 <211> 391 <212> PRT <213> pMALc2x-prodomain of sebastes schlegelii <400> 43 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 44 <211> 391 <212> PRT <213> pMALc2x-prodomain of oreochromis mossambicus (mozambiquetilapia) <400> 44 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 45 <211> 391 <212> PRT <213> pMALc2x-prodomain of pagrus major (redseabream) <400> 45 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 46 <211> 391 <212> PRT <213> pMALc2x-prodomain of acanthopagrus schlegelii (blackseabream) <400> 46 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 47 <211> 391 <212> PRT <213> pMALc2x-prodomain of takifugu rubripes (fugurubripes) <400> 47 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 48 <211> 391 <212> PRT <213> pMALc2x-prodomain of oncorhynchus mykiss (rainbowtrout) <400> 48 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 49 <211> 391 <212> PRT <213> pMALc2x-prodomain of salmo salar (atalantic salmon) myostatin 1a <400> 49 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 50 <211> 391 <212> PRT <213> pMALc2x-prodomain of pimephales promelas <400> 50 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 51 <211> 391 <212> PRT <213> pMALc2x-prodomain of Rotifer <400> 51 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe 385 390 <210> 52 <211> 659 <212> PRT <213> pMALc2x-prodomain of flatfish <400> 52 Met Lys Ile Glu Glu Gly Lys Leu Val Ile Trp Ile Asn Gly Asp Lys   1 5 10 15 Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys Lys Phe Glu Lys Asp Thr              20 25 30 Gly Ile Lys Val Thr Val Glu His Pro Asp Lys Leu Glu Glu Lys Phe          35 40 45 Pro Gln Val Ala Ala Thr Gly Asp Gly Pro Asp Ile Ile Phe Trp Ala      50 55 60 His Asp Arg Phe Gly Gly Tyr Ala Gln Ser Gly Leu Leu Ala Glu Ile  65 70 75 80 Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu Tyr Pro Phe Thr Trp Asp                  85 90 95 Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala Tyr Pro Ile Ala Val Glu             100 105 110 Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu Leu Pro Asn Pro Pro Lys         115 120 125 Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys Glu Leu Lys Ala Lys Gly     130 135 140 Lys Ser Ala Leu Met Phe Asn Leu Gln Glu Pro Tyr Phe Thr Trp Pro 145 150 155 160 Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe Lys Tyr Glu Asn Gly Lys                 165 170 175 Tyr Asp Ile Lys Asp Val Gly Val Asp Asn Ala Gly Ala Lys Ala Gly             180 185 190 Leu Thr Phe Leu Val Asp Leu Ile Lys Asn Lys His Met Asn Ala Asp         195 200 205 Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe Asn Lys Gly Glu Thr Ala     210 215 220 Met Thr Ile Asn Gly Pro Trp Ala Trp Ser Asn Ile Asp Thr Ser Lys 225 230 235 240 Val Asn Tyr Gly Val Thr Val Leu Pro Thr Phe Lys Gly Gln Pro Ser                 245 250 255 Lys Pro Phe Val Gly Val Leu Ser Ala Gly Ile Asn Ala Ala Ser Pro             260 265 270 Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu Asn Tyr Leu Leu Thr Asp         275 280 285 Glu Gly Leu Glu Ala Val Asn Lys Asp Lys Pro Leu Gly Ala Val Ala     290 295 300 Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys Asp Pro Arg Ile Ala Ala 305 310 315 320 Thr Met Glu Asn Ala Gln Lys Gly Glu Ile Met Pro Asn Ile Pro Gln                 325 330 335 Met Ser Ala Phe Trp Tyr Ala Val Arg Thr Ala Val Ile Asn Ala Ala             340 345 350 Ser Gly Arg Gln Thr Val Asp Glu Ala Leu Lys Asp Ala Gln Thr Asn         355 360 365 Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn Asn Asn Asn Leu Gly Ile     370 375 380 Glu Gly Arg Ile Ser Glu Phe Met His Leu Ser His Ile Val Leu Tyr 385 390 395 400 Leu Ser Leu Leu Val Ala Leu Gly Pro Val Val Leu Ser Asp Gln Glu                 405 410 415 Thr His His Gln Gln Pro Ser Ala Ser Ser Pro Glu Asp Ala Glu Gln             420 425 430 Cys Ala Thr Cys Asp Val Arg Gln Gln Ile Lys Thr Met Arg Leu Asn         435 440 445 Ala Ile Ile Ser Gln Ile Leu Ser Lys Leu Arg Met Lys Glu Ala Pro     450 455 460 Asn Ile Ser Arg Asp Ile Val Lys Gln Leu Leu Pro Lys Ala Pro Pro 465 470 475 480 Leu Gln Gln Leu Leu Asp Gln Tyr Asp Val Leu Gly Asp Asp Asn Arg                 485 490 495 Asp Val Val Met Glu Asp Asp Asp Glu His Ala Thr Thr Glu Thr Ile             500 505 510 Met Met Met Ala Thr Glu Pro Glu Pro Ile Val Gln Val Asp Ala Glu         515 520 525 Pro Lys Cys Cys Phe Phe Ser Phe Thr Gln Lys Phe Gln Ala Ser Arg     530 535 540 Ile Val Arg Ala Gln Leu Trp Val Tyr Leu Arg Pro Ala Asp Glu Ala 545 550 555 560 Thr Thr Val Phe Leu Gln Ile Ser Arg Leu Met Pro Val Thr Asp Gly                 565 570 575 Ser Arg His Ile Arg Ile Arg Ser Leu Lys Ile Asp Val Asn Ala Gly             580 585 590 Leu Ser Ser Trp Gln Ser Ile Asp Val Lys Gln Val Leu Thr Val Trp         595 600 605 Leu Arg Gln Pro Glu Thr Asn Trp Gly Ile Glu Ile Asn Ala Phe Asp     610 615 620 Ser Arg Gly Asn Asp Leu Ala Val Thr Ser Thr Glu Pro Gly Glu Glu 625 630 635 640 Gly Leu Gln Pro Phe Met Glu Val Lys Ile Thr Asp Gly Pro Lys Arg                 645 650 655 Val arg arg             <210> 53 <211> 167 <212> PRT <213> pET32a-prodomain of bostaurus <400> 53 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 54 <211> 167 <212> PRT <213> pET32a-prodomain of Norway rat <400> 54 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 55 <211> 167 <212> PRT <213> pET32a-prodomain of mouse <400> 55 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 56 <211> 167 <212> PRT <213> pET32a-prodomain of pig <400> 56 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 57 <211> 167 <212> PRT <213> pET32a-prodomain of chicken <400> 57 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 58 <211> 167 <212> PRT <213> pET32a-prodomain of turkey <400> 58 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 59 <211> 167 <212> PRT <213> pET32a-prodomain of sheep <400> 59 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 60 <211> 167 <212> PRT <213> pET32a-prodomain of human <400> 60 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 61 <211> 167 <212> PRT <213> pET32a-prodomain of dog <400> 61 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 62 <211> 167 <212> PRT <213> pET32a-prodomain of anasplatyrhynchos (mallard) <400> 62 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 63 <211> 167 <212> PRT <213> pET32a-prodomain of coturnix coturnix <400> 63 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 64 <211> 167 <212> PRT <213> pET32a-prodomain of macacafascicularis (cynomolgusmonkey) <400> 64 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 65 <211> 167 <212> PRT <213> pET32a-prodomain of gorilla gorilla gorilla (lowlandgorilla) <400> 65 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 66 <211> 167 <212> PRT <213> pET32a-prodomain of haliotis discus hannai <400> 66 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 67 <211> 167 <212> PRT <213> pET32a-prodomain of kareiusbicoloratus (stone flounder) <400> 67 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 68 <211> 167 <212> PRT <213> pET32a-prodomain of psetta maxima (turbot) <400> 68 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 69 <211> 167 <212> PRT <213> pET32a-prodomain of sebastes schlegelii <400> 69 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 70 <211> 167 <212> PRT <213> pET32a-prodomain of oreochromis mossambicus (mozambiquetilapia) <400> 70 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 71 <211> 167 <212> PRT <213> pET32a-prodomain of pagrus major (redseabream) <400> 71 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 72 <211> 167 <212> PRT <213> pET32a-prodomain of acanthopagrus schlegelii (blackseabream) <400> 72 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 73 <211> 167 <212> PRT <213> pET32a-prodomain of takifugu rubripes (fugurubripes) <400> 73 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 74 <211> 167 <212> PRT <213> pET32a-prodomain of oncorhynchus mykiss (rainbowtrout) <400> 74 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 75 <211> 167 <212> PRT <213> pET32a-prodomain of salmo salar (atalantic salmon) myostatin 1a <400> 75 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 76 <211> 167 <212> PRT <213> pET32a-prodomain of pimephales promelas <400> 76 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 77 <211> 167 <212> PRT <213> pET32a-prodomain of Rotifer <400> 77 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe                 165 <210> 78 <211> 435 <212> PRT <213> pET32a-prodomain of flatfish <400> 78 Met Ser Asp Lys Ile Ile His Leu Thr Asp Asp Ser Phe Asp Thr Asp   1 5 10 15 Val Leu Lys Ala Asp Gly Ala Ile Leu Val Asp Phe Trp Ala Glu Trp              20 25 30 Cys Gly Pro Cys Lys Met Ile Ala Pro Ile Leu Asp Glu Ile Ala Asp          35 40 45 Glu Tyr Gln Gly Lys Leu Thr Val Ala Lys Leu Asn Ile Asp Gln Asn      50 55 60 Pro Gly Thr Ala Pro Lys Tyr Gly Ile Arg Gly Ile Pro Thr Leu Leu  65 70 75 80 Leu Phe Lys Asn Gly Glu Val Ala Ala Thr Lys Val Gly Ala Leu Ser                  85 90 95 Lys Gly Gln Leu Lys Glu Phe Leu Asp Ala Asn Leu Ala Gly Ser Gly             100 105 110 Ser Gly His Met His His His His His His Ser Ser Gly Leu Val Pro         115 120 125 Arg Gly Ser Gly Met Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln     130 135 140 His Met Asp Ser Pro Asp Leu Gly Thr Asp Asp Asp Asp Lys Ala Met 145 150 155 160 Ala Asp Ile Gly Ser Glu Phe Met His Leu Ser His Ile Val Leu Tyr                 165 170 175 Leu Ser Leu Leu Val Ala Leu Gly Pro Val Val Leu Ser Asp Gln Glu             180 185 190 Thr His His Gln Gln Pro Ser Ala Ser Ser Pro Glu Asp Ala Glu Gln         195 200 205 Cys Ala Thr Cys Asp Val Arg Gln Gln Ile Lys Thr Met Arg Leu Asn     210 215 220 Ala Ile Ile Ser Gln Ile Leu Ser Lys Leu Arg Met Lys Glu Ala Pro 225 230 235 240 Asn Ile Ser Arg Asp Ile Val Lys Gln Leu Leu Pro Lys Ala Pro Pro                 245 250 255 Leu Gln Gln Leu Leu Asp Gln Tyr Asp Val Leu Gly Asp Asp Asn Arg             260 265 270 Asp Val Val Met Glu Asp Asp Asp Glu His Ala Thr Thr Glu Thr Ile         275 280 285 Met Met Met Ala Thr Glu Pro Glu Pro Ile Val Gln Val Asp Ala Glu     290 295 300 Pro Lys Cys Cys Phe Phe Ser Phe Thr Gln Lys Phe Gln Ala Ser Arg 305 310 315 320 Ile Val Arg Ala Gln Leu Trp Val Tyr Leu Arg Pro Ala Asp Glu Ala                 325 330 335 Thr Thr Val Phe Leu Gln Ile Ser Arg Leu Met Pro Val Thr Asp Gly             340 345 350 Ser Arg His Ile Arg Ile Arg Ser Leu Lys Ile Asp Val Asn Ala Gly         355 360 365 Leu Ser Ser Trp Gln Ser Ile Asp Val Lys Gln Val Leu Thr Val Trp     370 375 380 Leu Arg Gln Pro Glu Thr Asn Trp Gly Ile Glu Ile Asn Ala Phe Asp 385 390 395 400 Ser Arg Gly Asn Asp Leu Ala Val Thr Ser Thr Glu Pro Gly Glu Glu                 405 410 415 Gly Leu Gln Pro Phe Met Glu Val Lys Ile Thr Asp Gly Pro Lys Arg             420 425 430 Val arg arg         435

Claims (14)

Cloning the myostatin prodomain gene using myostatin of a biological tissue;
Inserting the biological myostatin prodomain into an expression vector;
Transforming the expression vector-binding myostatin prodomain into E. coli to express an recombinant protein of soluble myostatin prodomain by adding an expression inducing agent; And
Purifying the recombinant protein of the transformed soluble myostatin prodomain
Method for producing a recombinant protein of soluble myostatin prodomain that inhibits the function of myostatin consisting of.
The method of claim 1, wherein the myostatin prodomain is any one selected from terrestrial and marine organisms.
The method according to claim 1, wherein the myostatin prodomain is cow, rat, mouse, pig, chicken, turkey, sheep, human, dog, mallard, quail, Philippine monkey, roland gorilla, abalone, stone flounder, flounder, A method for producing a recombinant protein of soluble myostatin prodomain, characterized in that it is myostatin prodomain of zodiac rock, tilapia, red sea bream, black sea bream, volcano, trout, salmon, catfish, rotifer or flounder.
The method of claim 1, wherein the expression vector is selected from the group consisting of pET32a, pET43.a, pMALp2x and pMALc2x.
According to claim 1, wherein the E. coli is soluble myo, characterized in that selected from the group consisting of BL21 (DE3), BL21 (DE3) pLyss, origami2 (DE3), Origami2 (DE3) pLyss and Rogetta-gami2 (DE3) pLyss. Method for producing recombinant protein of statin prodomains.
The method of claim 1, wherein the protein expression inducing agent is 0.001-10 mM IPTG or 0.001-10% Lactose, characterized in that the recombinant protein production method of soluble myostatin prodomain.
Recombinant protein of soluble myostatin prodomain, characterized in that the recombinant protein of soluble myostatin prodomain that inhibits the function of myostatin of the organism produced by the method of claim 1 is added and expressed at 0 ℃ -50 ℃ Production method.
8. The recombinant protein of claim 7 wherein the recombinant protein of soluble myostatin prodomain is a pMALc2x-prodomain protein or a pET32a-prodomain protein.
The method of claim 8, wherein the pMALc2x-prodomain protein or pET32a-prodomain protein of the prodomain of the cow of SEQ ID NO: 1 to 26 (SEQ ID NO: 1), rat (SEQ ID NO: 2), mouse (SEQ ID NO: 3), pig (SEQ ID NO: 4), chicken (SEQ ID NO: 5), turkey (SEQ ID NO: 6), sheep (SEQ ID NO: 7), human (SEQ ID NO: 8), dog (SEQ ID NO: 9), mallard (SEQ ID NO: 10), quail (SEQ ID NO: 11), Philippine Monkey (SEQ ID NO: 12), Lowland Gorilla (SEQ ID NO: 13), Abalone (SEQ ID NO: 14), Stone Fluke (SEQ ID NO: 15), Fluke (SEQ ID NO: 16), Zoffevolak (SEQ ID NO: 17) , Tilapia (SEQ ID NO: 18), red sea bream (SEQ ID NO: 19), black sea bream (SEQ ID NO: 20), self-defense (SEQ ID NO: 21), rainbow trout (SEQ ID NO: 22), salmon (SEQ ID NO: 23), catfish (SEQ ID NO: 24) ), Recombinant protein of soluble myostatin prodomain, characterized in that the iostatin prodomain of rotifer (SEQ ID NO: 25) or halibut (SEQ ID NO: 26) .
The recombinant protein of soluble myostatin prodomain according to claim 8, wherein the pMALc2x-prodomain protein is a pMALc2x-prodomain protein selected from the group consisting of SEQ ID NOs: 27-52.
The recombinant protein of soluble myostatin prodomain according to claim 7, wherein the pET32a-prodomain protein is a pET32a-prodomain protein selected from the group consisting of SEQ ID NOs: 53-78.
A feed additive composition for promoting muscle growth or improving the growth rate of animals and plants containing a recombinant protein of soluble myostatin prodomain according to claim 7.
A pharmaceutical composition for preventing and treating muscle diseases, including muscular dystrophy, muscular dystrophy and muscular loss, which comprises the recombinant protein of soluble myostatin prodomain according to claim 7 to inhibit myostatin activity.
A health food composition for improving muscle growth, comprising the recombinant protein of soluble myostatin prodomain according to claim 7 as an active ingredient.

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