KR20100116750A - A livestock feed and a manufacturing method therefor - Google Patents

Abstract

PURPOSE: A livestock feed using mulberry leaf silage and a manufacturing method thereof are provided to manufacture the livestock which is highly qualified and appear the domestic animals antioxidant effect. CONSTITUTION: A mulberry silage 40~50 is mixed by concentrate feed of weight whole-crop barley 2.5~7.5% weight; DDGS 2.5~7.5 weight; corn gluten feed 5~1 5% weight; cracked corn grain 5~15% weight; flake 2.5~7.5% weight; beet pulp 2.5~7.5% weight; ryegrass 2.5~7.5% weight; corn stalk pellet 2.5~7.5% weight and salt 0.1~0.5% weight.

Description

Livestock feed and A Manufacturing Method therefor}

The present invention relates to a livestock feed and a method for producing the same, and more particularly, to a livestock feed and an additional method for producing an antioxidant function.

In general, livestock must feed their physiological forages, and as a result, they feed forages such as crests, hay, rice husks, cottonseeds, beet pulp, and corncobs.

In other words, the feed that maintains the life of the livestock and supplies the organic or inorganic nutrients necessary for producing milk, meat, eggs, fur, etc. is largely divided into feed and rich feed, and the feed is characterized by high content of crude fiber and rich feed. In contrast to forages, crude fiber has a low content, while protein and soluble minerals are high, which can increase livestock production and livestock production efficiency.

However, the above-described prior art has the following problems.

Since the compound feed is simply to provide nutrients to the livestock, in order to promote the health of the livestock, there is a problem in that the feed cost is increased because a separate chemical is injected.

Accordingly, it is an object of the present invention to solve the problems of the prior art as described above, and to provide a livestock feed and a method for producing the same that exhibits the functionality of health promotion of the livestock.

According to a feature of the present invention for achieving the object as described above, according to the present invention livestock feed manufacturing method, it can comprise livestock feed including mulberry silage.

And, the production method of the livestock feed according to another feature of the present invention, mulberry silage 40-50 parts by weight; 2.5-7.5 parts by weight of barley; DDGS 2.5-7.5 parts by weight; 5-15 parts by weight of protein skin; 5-15 parts by weight of sapphire chains; Flakes 2.5 to 7.5 parts by weight; Beet pulp 2.5 to 7.5 parts by weight; Lygras 2.5 to 7.5 parts by weight; Octape pellet 2.5 to 7.5 parts by weight; 0.1-0.5 parts by weight of I-salt; 0.1 to 0.5 parts by weight of limestone; 0.2 to 0.6 parts by weight of urea; 0.3 to 0.7 parts by weight of PReMIX; And, molasses 2.5 to 5.0 parts by weight; can be configured to include.

And, the production method of the livestock feed according to another feature of the present invention, mulberry silage 40-50 parts by weight; 2.5-7.5 parts by weight of barley; DDGS 2.5-7.5 parts by weight; 5-15 parts by weight of protein skin; 5-15 parts by weight of sapphire chains; Flakes 2.5 to 7.5 parts by weight; Beet pulp 2.5 to 7.5 parts by weight; Lygras 2.5 to 7.5 parts by weight; Octape pellet 2.5 to 7.5 parts by weight; 0.1-0.5 parts by weight of I-salt; 0.1 to 0.5 parts by weight of limestone; 0.2 to 0.6 parts by weight of urea; 0.3 to 0.7 parts by weight of PReMIX; And, molasses 2.5 to 5.0 parts by weight; may be characterized in that the rich feed is mixed with the forage consisting of.

And, the present invention can be characterized by the livestock feed produced by the above-described method.

Livestock feed according to the present invention and its manufacturing method has the following effects.

By preparing a feed using mulberry leaf silage containing 1-deoxynojirimycine (DNJ), the animals that consume the feed have an antioxidant effect, which can produce high quality livestock products.

Hereinafter, preferred embodiments of a livestock feed and a method of manufacturing the same according to the present invention will be described in detail with reference to the accompanying drawings.

Livestock feed according to the present invention is configured to include mulberry silage. The mulberry leaf silage is to impart functionality to livestock feed, more preferably for the antioxidant effect. The mulberry silage is suitable as a livestock feed as shown in the experimental example below, it will exhibit antioxidant functionality.

Experiment 1: Component Analysis and Verification of Vegetarian Diet in Functional Custom TMR Feeds

end. Active ingredient of mulberry silage

Table 1 shows the results of analyzing active ingredients of mulberry silage. The content of 1-deoxynojirimycin (DNJ), a blood sugar-enhancing component of mulberry silage, was analyzed using the method of Stead and Richards (1996), which showed 0.568 mg / g. This is somewhat lower than the result of DNJ analysis of mulberry leaves by type of mulberry in Chae et al. (2003) .In the experiment of Chae et al. (2003), only the mulberry leaf was analyzed. The difference seems to be the result of silage analysis.

Analysis of Effective Ingredients of Mulberry Silage ingredient content Remarks (Unit) 1-deoxynojirimycine
(DNJ) 0.568 mg / g GABA Total flavonoids - μg / mg
Total phenols 21.695 μg / mg

The content of GABA (-Aminobutyric acid) was analyzed by Bang et al. (1998). Flavonoids were not detected in this study and total phenol content of 21.69μg / mg was analyzed. Mulberry leaves differed slightly depending on the variety, but it was reported that flavonoid and phenol were contained (Chae et al., 2003). In this study, phenol was detected but flavonoid was not analyzed. Flavonoid is a kind of poly-phenolic compound, and it is estimated to be contained in a certain amount of phenol component. In this study, it was analyzed for the whole part of the mulberry tree. Therefore, if a very small amount is contained, some components may not be detected. Judging.

  However, the mulberry silage used in this study also contains various active ingredients in mulberry leaves, which is considered to be sufficient as a functional feed.

I. Functional Evaluation of Mulberry Silage

1 and 2 are to evaluate the antioxidant function of Hydroxyl radical scavenging and Alkyl radical scavenging to evaluate the functionality of the mulberry silage used in the experiment. Hydroxyl radical scavenging activity was 0.5-2.0mg / ml and the higher the concentration, the greater the antioxidant effect. At 2.0mg / ml, the activity was over 50%.

Alkyl radical scavenging activity showed higher antioxidant effect at lower concentrations, and showed more than 50% activity effect at concentration of 0.125mg / ml.

Through these active effects, the antioxidant function of mulberry silage was fully demonstrated, and based on this, it can be considered to have the meaning of functional livestock feeding of mulberry silage.

And, the livestock feed according to the present invention, 40-50 parts by weight of mulberry silage, 2.5-7.5 parts by weight of barley, 2.5-7.5 parts by weight of DDGS, 5-15 parts by weight of protein skin, 5-15 By weight, 2.5-7.5 parts by weight of flakes, 2.5-7.5 parts by weight of beet pulp, 2.5-7.5 parts by weight of lygras, 2.5-7.5 parts by weight of pellets, 0.1-0.5 parts by weight of I-salt, limestone 0.1 to 0.5 parts by weight, 0.2 to 0.6 parts by weight of urea, 0.3 to 0.7 parts by weight of PReMIX, and 2.5 to 5.0 parts by weight of molasses.

To the livestock feed by the composition, a small amount of enteric gourd, rice straw and added water may be further added. As described above, the composition of the livestock feed is administered to actual cattle, that is, Korean cattle. Preferably it may be formulated as a blending ratio in Table 2. In addition, it is also possible to use a feed prepared in the blending ratio of Table 2 as a feed material and mixed with a rich feed.

<Experiment 2: Development of Functional Custom TMR Model Using Mulberry Silage>

In order to develop a customized TMR feed for the production of high-quality beef cattle using mulberry silage, we responded to the production of high-quality beef cattle through the following combination ratio (Table 2). The feed was fed at the late stage of finishing at a high-quality beef cattle farmer.

Compounding cost No. Product Name  building TMR RV Unit price (KRW)  ratio(%)  Input (kg)  price RV moisture Building quantity One Mulberry Leaf Silage 40 0.80  300   45.0   1,800.0 135.0 0.36 27.0 18.0 2 Barley 29 0.90  235    5.0    200.0   11.8 0.05  3.6  1.5 3 DDGS 89 0.40  330    5.0    200.0   16.5 0.02  0.6  4.5 4 Jang Yoo Park 60 0.40  188     -       -     -   -    -    - 5 Protein 90 0.40  280   10.0    400.0   28.0 0.04  1.0  9.0 6 Sapphire 88 0.40  430   10.0    400.0   43.0 0.04  1.2  8.8 7 Flake 89 0.40  490    5.0    200.0   24.5 0.02  0.6  4.5 8 Beet pulp 90 0.50 260    5.0    200.0   13.0 0.03  0.5  4.5 9 Ryegrass 88 1.05 340    5.0    200.0   17.0 0.05  0.6  4.4 10 Crested straw 88 1.05 123      -        -     -   -    -     - 11 Jade pellet 88 0.80 245    5.0    200.0  12.3  0.04  0.6  4.4 12 I-SALT 94 0.10 117    0.3    12.0   0.4  0.00  0.0  0.3 13 Limestone 95 0.00  35    0.3    12.0   0.1   -  0.0  0.3 14 Element 96 0.10 625    0.4    16.0  2.5 0.00  0.0  0.4 15 PReMIX 90 0.10 1,500    0.5    20.0  7.5 0.00  0.1  0.5 16 molasses 35 0.00  225   3.5    140.0  7.9  -  2.3  1.2 17 Water added  One 0.00     5    -       -    -   -   -    - Raw material cost   100.0  4,000.0 319.3 0.64 37.9   62.1

<Table 3> shows the general content of TMR feed using mulberry silage. The dry matter content was 71.6%. The crude protein content was 12.4%, the crude fat was 2.5%, and the crude fiber content was 20.3%. Under these feed conditions, feeding was conducted to beef cattle for cattle, and the experiment was conducted. Compared with the conventional Hanwoo beef feed, the experimental feed was only slightly different in crude protein content, and it was evaluated that there would be no problem in using it as a feed for the abundant feed due to sufficient feed value.

Component Analysis Table of Mulberry TMR Feed Feed composition Building (DM) Crude protein
(CP) Crude fat
(EE) Crude fiber
(CF) View minutes
(Ash) Analysis
(DM%) 71.6 12.4 2.5 20.3 6.9

 <Table 4> is a record of feed and vegetarian food per 1 cow per day during the experimental period. The food was processed according to the fattening program, and most of the feed was vegetarian, so there was no problem in palatability. Could know. Since all 8 cows used in the experiment were vegetarians with the whole diet, it could be considered that it would have no effect on palatability and productivity.

Mulberry Silage TMR Feed and Vegetarian Diet individual
number December 22 December 28 5 January 12 Jan Salary
(kg) Vegetarian
(kg) Salary
(kg) Vegetarian
(kg) Salary
(kg) Vegetarian
(kg) Salary
(kg) Vegetarian
(kg) One 13.5 13.5 11.5 11.5 12.5 12.5 11.5 11.5 2 13.5 13.5 11.5 11.5 12.5 12.5 11.5 11.5 3 13.5 13.5 11.5 11.5 12.5 12.5 11.5 11.5 4 13.5 13.5 11.5 11.5 12.5 12.5 11.5 11.5 5 13.5 13.5 11.5 11.5 12.5 12.5 11.5 11.5 6 13.5 13.5 11.5 11.5 12.5 12.5 11.5 11.5 7 13.5 13.5 11.5 11.5 12.5 12.5 11.5 11.5 8 13.5 13.5 11.5 11.5 12.5 12.5 11.5 11.5

<Figure 3> is a record of the specification management of the Hanwoo published for the experiment was placed four heads in one ally provided enough space and there was no shortage of feed intake.

<Experiment: Production of Hanwoo Beef by Three Function Customized TMR Feed (Productivity Verification)>

The results of the meat quality evaluation as a means for evaluating the productivity of Hanwoo according to the feed of TMR feed using mulberry silage are shown in Figures 4 and 5. In consideration of the risk of the experiment (stress factor for livestock), the results of the experiment on the general Korean cattle, which are somewhat far from the highest grade meat production, were mostly B1 +, B1, and B2 grades in the group fed the experimental diet. It was analyzed that the meat that produced the meat was difficult enough to be graded in the experimental group. In the group fed general feed, C1 and B2 grades were the majority.

  Therefore, the availability of TMR feed for the production of high-quality beef cattle using mulberry silage is considered to be sufficient, and it is expected to be commercialized as a new product.

<Experiment: Measurement of productivity of Korean cattle by four-function customized TMR feed (Verification of livestock hygiene and safety)>

end. Microbial Status in Feces of Hanwoo Beef

Feeding TMR Feeds Using Mulberry Silage The results of intestinal microbial testing to evaluate livestock hygiene in beef cattle are shown in <Table 5>. <Figure 6> shows the feces taken immediately after the distribution for microbial examination in the intestine through feces and stored in the refrigerator for analysis. Fecals were collected four times at weekly intervals from the start of the experiment, and each individual was collected at the site immediately after distribution and transferred to a test facility after refrigerated storage for microbial count. The microorganisms were tested against the number of bacteria, Salmonella and E. coli, and the test results are shown in <Table 5>.

Status of Microorganisms in Feces of Mulberry Silage TMR Feed Hanwoo Sampling date General bacteria
(cfu / ml) Salmonella
(cfu / ml) Escherichia coli
(cfu / ml) Control Treated group Control Treated group Control Treated group Dec 29, 2008 1.28 × 10 ⁵ 1.15 × 10 ⁵ Not detected Not detected Not detected Not detected 2009. 01. 05. 3.4 × 10 ⁶ 9.3 × 10 ⁵ Not detected Not detected Not detected Not detected 2009. 01. 12. 1.18 × 10 ⁶ 9.8 × 10 ⁵ Not detected Not detected 2.3 × 10 ³ 7.0 × 10 ³ 2009. 01. 19. 4.5 × 10 ⁶ 1.7 × 10 ⁶ Not detected Not detected Not detected 5.0 × 10 ²

* Control group: general feed, treatment group: mulberry silage pay group

The number of normal bacteria in the control group of control group and control group of mulberry silage was slightly different according to sampling time, but the control group was 1.15 × 10 ⁵ cfu / mg and the control group was 1.28 ×. 10 ⁵ cfu / mg showed a slightly lower number of common bacteria in the treatment group, but Salmonella and E. coli were not detected.

In the fecal sample of the second sample, the control group showed 3.4 × 10 ⁶ cfu / mg and the control group 9.3 × 10 ⁵ cfu / mg, which also showed a significantly lower bacterial count in the treated group, and Salmonella and E. coli were not detected, respectively.

Also, in the fecal sample collected from the 3rd sample, the control group was 1.18 × 10 ⁶ cfu / mg, the control group was 9.8 × 10 ⁵ cfu / mg, and Salonella was not detected, but the E. coli was control group 2.3 × 10 ³ cfu / mg, the treatment group. The 7.0 × 10 ³ cfu / mg was detected, but this was also lower in the treated group than in the control group.

In the fecal sample collected from the 4th sample, the control group showed a bacteria count of 1.7 × 10 ⁶ cfu / mg in the control group of 4.5 × 10 ⁶ cfu / mg, and the number of general bacteria in the intestinal tract in the feces of mulberry silage TMR feed fed Hanwoo control The figure was significantly lower than that. As a result of analyzing the status of microorganisms in the intestinal tract of Hanwoo in this specification test, it was analyzed that Salmonella and Escherichia coli were lower than general feed group, so that mulberry silage TMR feed was functional in terms of livestock hygiene. It became.

I. Changes in Blood Characteristics of Hanwoo Beef with Mulberry Silage TMR Feed

Table 6 shows the results of blood collection from the vein before and after the experiment to examine the changes in blood characteristics of Hanwoo beef cattle by feeding the mulberry silage TMR feed. <Figure 7> is a scene of collecting blood from the vein of the Han cattle, which is the experimental livestock, at the start and end of the experiment. The collected blood was refrigerated in the form of whole blood containing anticoagulant and centrifuged to separate the serum, and then frozen and stored for analysis.

Blood Characteristics of Hanwoo Beef with Mulberry Silage TMR Feed Item Before the experiment After the experiment Average Standard Deviation Average Standard Deviation T-protein 37.6 2.3 37.1 3.0 Albumin 11.9 0.6 11.8 0.6 T-cholesterol 420.4 18.5 402.9 26.2 HDL 26.6 6.6 23.1 6.9 Triglyceride 797.6 57.6 803.0 84.2 glucose 240.0 6.2 215.6 14.1 Urea 6.8 3.6 7.9 5.3 D-bilirubun 6.4 1.0 5.6 0.7

T-protein showed little difference between before and after experiment, and Albumin showed similar tendency. In the analysis of various blood physicochemical components, there was almost no difference between before and after the experiment, indicating that there was no problem in the adaptability of mulberry silage TMR feed. Basically, the physicochemical components in the blood are greatly changed by the components of the feed. In this experiment, although mulberry silage was used, it was basically formulated as a feed for beef cattle, especially based on the nutrient content recommended in the beef cattle program. Therefore, the main reason is that there was no big difference between the diet and the nutritional content. Therefore, the experimental feed also does not have a significant effect on the livestock, so it has been found that there is no major problem in the productivity of the beef cattle.

<Experiment: 5. Identification of Active Components and Functional Properties of Hanwoo Beef with Mulberry Silage TMR Feed>

end. Summary of methods for verifying antioxidant efficacy

( 1) Analysis reagent

5,5-Dimethyl-1-pyrroline N-oxide (DMPO), 2,2-azobis (2-amidinopropane) used for free radical scavenging activity using an electron spin resonance (ESR) device to verify the antioxidant efficacy of this experiment hydrochloride (AAPH), 1,1-diphenyl-2-picrylhydrazyl (DPPH) and (4-pyridyl-1-oxide) -N-tert-butylnitrone (4-POBN) and catalase, glutathione, Glutathione peroxide and glutathione-S-transferase kits were purchased from Sigma, and Fluka's superoxide dismutase kit was used to measure antioxidant activity. All other analytical reagents used express reagents.

(2) Method of measuring antioxidant enzyme activity

1) Determination of Superoxide Dismutase (SOD) Activity

The sample was homogenized three times for 1 minute on ice, and then centrifuged twice for 15 minutes using a centrifuge to quantify the amount of protein using the supernatant. 0.2 ml of jacking solution and 0.02 ml of enzyme working solutions were added to the sample solution with a blank (distilled water was used instead of the sample on the blank) for 20 minutes at 37 ° C, and the absorbance was measured at 450 nm using a spectrophotometer.

2) Determination of Glutathione Activity

10 μl of sample solution and 150 μl of working mixture were added to 5% 5-Sulfosalicylic acid (SSA), and 50 μl of NADPH (0.16 mg / ml) was added. After 5 minutes, the absorbance was measured at 412 nm using a spectrophotometer.

3) Glutathione S-transferase (GST) activity measurement

Substrate solution and sample solution were added to the test tube, mixed, and then absorbance was measured at 340 nm using a spectrophotometer.

4) Determination of Glutathione peroxidase (GPx) activity

Add 50 ~ 1 μl of NADPH assay reagent, mix 20 ~ 50 μl of enzyme (0.25 units / ml) and 10 ~ 50 μl of sample solution, fill with 1 ml of GPx assay buffer, and use a spectrophotometer. Absorbance was measured at 340 nm.

5) Catalase (CAT) activity measurement

After mixing 75 μl of analytical buffer solution and 25 μl of a colorimetric substrate solution with a sample of a certain concentration, 10 μl of the reaction stop solution and 1 ml of a coloring reagent were left for 15 minutes and absorbed at 520 nm using a spectrophotometer. Measured.

( 3) Method for measuring free radical scavenging activity using an electronic spin resonance apparatus

1) Determination of Hydroxyl Radical Scavenging Activity by Electron Spin Resonance Equipment (ESR)

The hydroxyl radical scavenging activity of each sample was dissolved in 0.2 ml distilled water to a constant concentration, followed by 0.2 ml of 0.3 M 5,5-dimethyl-1-pyrroline N-oxide (DMPO), 0.2 ml of 10 mM FeSO ₄ and 10 mM. 0.2 ml of H ₂ O ₂ /0.1 M phosphate buffer (pH 7.4) were mixed and allowed to stand at room temperature for 2.5 minutes and then transferred to a quartz capillary tube, which was measured by an electron spin resonance (ESR) spectrometer. Spectra were recorded under the conditions of scan time: 200s, field: 3461.3 ± 50 G, time constant: 0.5s, power: 1mW, amplitude: 1 * 200. The scavenging activity of hydroxyl radical for each sample was Calculated by

Hydroxyl radical scavenging activity (%) = 100-(ESR signal intensity for medium containing the samples / ESR signal intensity for the control medium) * 100.

2) Superoxide radical scavenging activity

Superoxide radical scavenging activity of each sample was measured from hypoxanthine (HPX) -xanthine oxidase (XOD) system. That is, mix 50 μl of 4 mM HPX, 30 μl phosphate buffer saline (PBS), 50 μl sample solution, 20 μl of 4.5 M DMPO and 50 μl XOD (0.4 U / ml), and transfer the solution to a quartz capillary tube. After 45 seconds it was measured by ESR. The spectrum was recorded under the conditions of field: 337.1 ± 5 mT, power: 4 mW, scan time: 2 min, amplitude: 1 * 500, time constant: 0.3 s. The scavenging activity of superoxide anionl radical for each sample was Calculated using the formula.

Superoxide anionl radical scavenging activity,% = 100-(ESR signal intensity for medium containing the additives of concern / ESR signal intensity for the control medium) * 100.

3) Measurement of DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity

DPPH radical scavenging activity of each sample was measured by ESR after adding 60 μl DPPH solution (60 μM) to 60 μl sample solution for 10 seconds and then transferring the mixed solution to a quartz capillary tube. The spectrum was recorded under the conditions of field: 337.1 ± 5 mT, power: 4 mW, scan time: 2 min, amplitude: 1 * 500, time constant: 0.3 s. Calculated using.

DPPH radical scavenging activity,% = 100-(ESR signal intensity for medium containing the samples / ESR signal intensity for the control medium) * 100.

4) Alkyl radical scavenging activity measurement

The alkyl radical scavenging activity of each sample was 10 mM AAPH (2,2'-azobio (2-amidinopropane) hydrochloride), 10 mM 4-POBN (a- (4-pyridyl-1-oxide) -N - t -bytylnitrone) And the phosphate-buffered saline (pH 7.4) reactants containing various concentrations of the sample was incubated at 37 ℃ for 30 minutes, the mixed solution was transferred to a quartz capillary tube and measured by ESR. The spectrum was recorded under the conditions of field: 337.1 ± 5 mT, power: 4 mW, scan time: 2 min, amplitude: 1 * 500, time constant: 0.3 s. Calculated using.

Alkyl radical scavenging activity,% = 100-(ESR signal intensity for medium containing the samples / ESR signal intensity for the control medium) * 100.

I. Antioxidant measurement result

(1) SOD activity

Superoxide anion, which is indispensable through respiration, is converted to hydrogen peroxide by superoxide dismutase. As a result of measuring the SOD activity divided into the control group and the experimental group in the present results, as shown in FIG.

(2) Glutathione activating effect

The hydroxyl radical produced in vivo is one of the most powerful radicals, destroying DNA, proteins, lipids, and causing cell death. At this time, there are GSH, GST, and GPx to generate water by reducing hydroxyl radicals. As shown in FIG. 9, the results showed that the glutathione activity was 22.73 μmol / ml in the experimental group compared to the control group (16.55 μmol / ml) as a result of measuring Glutathaione activity.

(3) Glutathine S-transferase Activity

As shown in Figure 10, the result of measuring the activity of Glutathaione S-transferase showed a high activity of 8.24 μmol / ml / min in the experimental group compared to 3.65 μmol / ml / min in the control group about 2.3 times higher activity in the experimental group Indicated.

(4) Glutathione peroxidase activating effect

The results of verifying the effect of Glutathione peroxidase activity on Hanwoo meat produced by feeding the experimental feed were as follows.

As a result of measuring the activity of glutathione peroxidase, the control group exhibited a high activity of 0.376 μmol / ml / min in the experimental group compared to 0.275 μmol / ml / min in the control group, which was 1.4 times higher than the control group.

(5) Catalase activity effect

 The results of catalase activity on Hanwoo meat produced by feeding experimental feed were as follows.

The control group showed 0.108 μmol / ml and the experimental group showed 0.160 μmol / ml, and the experimental group showed 1.5 times higher activity than the control group. Therefore, as can be seen from the results of sulfate enzyme activity, the antioxidant enzyme activity was better in the Hanwoo group fed the mulberry silage TMR feed than the Hanwoo fed the normal feed, which is the Hanwoo group fed the mulberry silage. It is believed to be superior in aging inhibition and disease incidence.

(6) DPPH radical scavenging activity effect

Results of verifying the DPPH radical scavenging activity effect on the Hanwoo meat produced by feeding the experimental feed is shown in <13> and <Figure 14>. As a result of measuring DPPH radical scavenging activity, the IC ₅₀ value of the control group was 171 μg and the experimental group was 98 μg.

<Figure 14> shows the result of eliminating the signal of DPPH radical and the signal of DPPH radical according to the addition of Hanwoo beef in the electron spin resonator. The signal of DPPH radical increases as the concentration of the extract extracted from Hanwoo beef increases. Decreased, indicating an increase in DPPH radical scavenging activity.

(7) Hydroxyl radical scavenging activity

FIG. 15 shows the hydroxyl radical scavenging activity of the sample extract. The IC ₅₀ values of the control group and the experimental group were 124 μg and 88 μg, indicating that the experimental group had about 1.5 times higher hydroxyl radical scavenging activity than the control group.

<Figure 16> shows a typical signal of the hydroxyl radical in the electron spin resonance device, the hydroxyl radical shows a typical signal of 1: 2: 2: 1, the scavenging activity of the hydroxyl radical with increasing concentration of the sample extract It was found to increase.

(8) Scavenging Effect of Alkyl Radicals

17 shows the results of measuring the scavenging activity of the Alkyl radicals, and the IC ₅₀ values of the control group and the experimental group were 334 μg and 180 μg, indicating that the experimental group was about twice as high as the control group.

FIG. 18 shows that the signal decreases as the concentration of the sample extract increases as an intrinsic signal of the Alkyl radical.

(9) Superoxide radical scavenging activity

19 shows the results of the superoxide radical scavenging activity. The IC ₅₀ values of the control group and the experimental group were 965 μg and 665 μg, indicating that the experimental group had about 1.5 times higher superoxide radical scavenging activity than the control group.

20 shows a unique signal possessed by Superoxide radical 1, and the signal was decreased as the concentration of the sample extract was increased, which means that the superoxide radical scavenging activity was increased as the concentration of the sample was increased.

<Experiment 6: Functional Evaluation by NMR Analysis of Hanwoo Beef Produced by Mulberry Silage TMR Feed Supplementation

end. Overview of NMR Analysis of Korean Beef

Figures 21 and 22 show a schematic diagram of the NMR analysis method and process for the meat produced from mulberry silage TMR feed salary Hanwoo (treatment) and the meat produced from normal Hanwoo (control).

I. Hanwoo Meat Sample Processing Method for NMR Analysis

(1) collection of analyzed meat

Analytical Hanwoo meat was fed to mulberry silage TMR feed at the experimental farm, followed by speciation experiments, and 10 samples of Hanwoo meat (control) were used for analysis.

(2) Experiment method

1) Hanwoo beef used 100mg of powder obtained by grinding after freeze drying.

2) 100 mg of Korean beef was extracted from 10 mM sodium phosphate (D ₂ O: CD ₃ OD = 1: 1) pH6.0 buffer.

3) The 1D spectrum of the extracted samples was obtained from Bruker 500MHz NMR.

4) 1D NMR data were analyzed using OPLS-DA method.

All. Development of Korean Beef Meat Discrimination Analysis Model by NMR Spectroscopy

(1) NMR data acquisition result

1) 1D NMR spectra raw data

FIG. 23 shows one 1D NMR spectrum of each sample of mulberry silage TMR feed salary Hanwoo meat (treated) and normal feed salary Hanwoo meat (control). Each spectrum shows -0.1ppm ~ 11ppm. The 0 ppm peak of the spectrum represents TSP, the reference standard. The spectrum is divided into aromatic (about 11ppm ~ 5ppm), sugar (5ppm ~ 3ppm), and aliphatic region (3ppm ~ 0ppm). The 1D NMR spectrum of Hanwoo meat due to the difference in feed was slightly different in sugar and aliphatic parts.

(2) Results of establishing multivariate statistical analysis model using OPLS-DA

1) Statistical Analysis of Feeding Hanwoo Beef with Mulberry Silage TMR Feed

Figure 24 shows the analysis results of Hanwoo meat by the difference in salary feed using the OPLS-DA analysis. This method is not an analysis method for individual samples, but an analysis method for two groups. Mulberry Silage TMR Feed Salary Hanwoo (red circle) and regular feed Salmon (black) show that the two groups are well separated around the Y axis.

Figure 25 shows the S plot for the OPLS-DA analysis of mulberry silage TMR feed Hanwoo beef and normal feed Hanwoo meat. The S-plot provides information on which signals are used to determine the grouping in the OPLS-DA analysis.

25A shows the S plot for the overall chemical shift. From this S-plot, it can be seen that the signals of 4.1 ppm and 3.89 ppm are marker signals that make a significant contribution to distinguishing the two groups. The difference in average intensity for each of the ten samples for the labeled signals is shown in FIGS. 26B and C, respectively.

Therefore, Hanwoo meat produced by feeding the mulberry silage TMR feed through these analysis was able to distinguish clearly from the Hanwoo meat produced by feeding the normal feed. This proves that dietary feed affects metabolic changes in vivo.

The rights of the present invention are not limited to the embodiments described above, but are defined by the claims, and those skilled in the art can make various modifications and adaptations within the scope of the claims. It is self-evident.

1 is a graph showing the antioxidant (Hydroxyl radical scavenging activity) efficacy of the mulberry silage constituting the animal feed of the present invention.

Figure 2 is a graph showing the antioxidant (Alkyl radical scavenging activity) efficacy of mulberry silage.

Figure 3 is an embodiment showing a breeding and feed vegetarian scene of livestock feed according to the present invention.

Figure 4 is an embodiment showing the meat grade of the present inventors livestock feed mulberry silage TMR feed Hanwoo.

Figure 5 is an embodiment showing the meat grade of normal feed salary (control) Hanwoo.

Figure 6 is an embodiment showing a fecal collection and refrigeration storage scene of the experimental livestock according to the present invention.

Figure 7 is an embodiment showing the blood collection surface of the experimental livestock administered the livestock feed according to the present invention.

8 is a graph showing the SOD activity effect of the meat produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

9 is a graph showing the glutathione activity effect of the meat produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

Figure 10 is a graph showing the effect of Glutathione S-transferase activity of the meat produced from livestock feed mulberry silage TMR feed Hanwoo cattle according to the present invention.

Figure 11 is a graph showing the effect of Glutathione peroxidase activity produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

12 is a graph showing the effect of Catalase activity of the meat produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

Figure 13 is a graph showing the effect of DPPH radical scavenging activity of meat produced from livestock feed mulberry silage TMR feed feed Hanwoo cattle according to the present invention.

14 is a graph showing the effect of DPPH radical scavenging activity of the meat produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

15 is a graph showing the effect of hydroxyl radical scavenging activity of the meat produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

Figure 16 is a graph showing the effect of hydroxyl radical scavenging activity of the meat produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

17 is a graph showing the effect of Alkyl radical scavenging activity of the meat produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

18 is a graph showing the effect of Alkyl radical scavenging activity of the meat produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

19 is a graph showing the effect of Superoxide radical scavenging activity of the meat produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

20 is a graph showing the effect of superoxide radical scavenging activity of the meat produced from the mulberry silage TMR feed feed Hanwoo cattle of the present invention.

Figure 21 is a schematic diagram of the NMR analysis method of Hanwoo beef fed livestock feed according to the present invention.

Figure 22 is a schematic diagram of the NMR analysis process of Hanwoo beef fed livestock feed according to the present invention.

Figure 23 is a graph showing the NMR 1D spectrum for the beef cattle fed livestock feed according to the present invention.

Figure 24 is a graph showing the OPLS-DA analysis of the sample in Hanwoo beef fed livestock feed according to the present invention.

25 is a comparison table of the control and treatment OPLS-DA S plot and labeling signal for the feed of the cattle beef fed livestock feed according to the present invention. (A) total chemical shift, (B) intensity for 4.1 ppm signal, (C) intensity for 3.89 ppm signal

Claims (4)

Method of producing livestock feed comprising mulberry silage. Mulberry silage 40-50 parts by weight; 2.5-7.5 parts by weight of barley; DDGS 2.5-7.5 parts by weight; 5-15 parts by weight of protein skin; 5-15 parts by weight of sapphire chains; Flakes 2.5 to 7.5 parts by weight; Beet pulp 2.5 to 7.5 parts by weight; Lygras 2.5 to 7.5 parts by weight; Octape pellet 2.5 to 7.5 parts by weight; 0.1-0.5 parts by weight of I-salt; 0.1 to 0.5 parts by weight of limestone; 0.2 to 0.6 parts by weight of urea; 0.3 to 0.7 parts by weight of PReMIX; And, molasses 2.5 ~ 5.0 parts by weight; production method of a livestock feed comprising a. Mulberry silage 40-50 parts by weight; 2.5-7.5 parts by weight of barley; DDGS 2.5-7.5 parts by weight; 5-15 parts by weight of protein skin; 5-15 parts by weight of sapphire chains; Flakes 2.5 to 7.5 parts by weight; Beet pulp 2.5 to 7.5 parts by weight; Lygras 2.5 to 7.5 parts by weight; Octape pellet 2.5 to 7.5 parts by weight; 0.1-0.5 parts by weight of I-salt; 0.1 to 0.5 parts by weight of limestone; 0.2 to 0.6 parts by weight of urea; 0.3 to 0.7 parts by weight of PReMIX; And, molasses 2.5 ~ 5.0 parts by weight; method of producing a livestock feed, characterized in that for mixing the rich feed to the feed consisting of. Livestock feed prepared by the method according to any one of claims 1 to 3.

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