KR20100104611A - Cosmetic composition containing horse chestnut extract for improving dark circle - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Horse chestnut extract for relieving dark circle is provided to suppress angitotensin I converting enzyme and to promote blood circulation on skin. CONSTITUTION: A cosmetic composition for relieving dark circle contains 0.001-30.0 weight% of Horse chestnut extract as an active ingredient. A cosmetic composition for suppressing angiotensin I converting enzyme contains Horse chestnut extract as an active ingredient. A cosmetic composition for enhancing capillary blood vessel and promoting blood circulation contains Horse chestnut extract as an active ingredient. The cosmetic composition is used in the form of skin softener, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water pack, spray, or powder.

Description

Cosmetic composition for relieving dark circles containing horse chestnut extract {Cosmetic Composition containing Horse chestnut extract for improving dark circle}

The present invention relates to a cosmetic composition for relieving dark circles, and more particularly, contains a horse chestnut extract as an active ingredient, inhibits angiotensin I converting enzyme, and has an excellent effect on inhibiting the production of angiotensin II, and skin purity of the skin. It relates to a cosmetic composition for dark circle relaxation that can be promoted.

Dark circles mean that the blood vessels around the eyes appear dark through thin skin. It is most likely to occur if blood circulation does not go around the eyes due to fatigue, lack of sleep, or stress. In addition, the area around the eyes is thinner and more sensitive than other areas, which can lead to darker circles as the skin loses elasticity and fat tissue grows. Dark circles darken facial expressions and make them look older.

Dark circles are caused by a combination of causes. First, the skin appears darkened by capillaries under the eyes. Secondly, fat under the eyes accumulates and sags downward, forming shadows, resulting in dark circles. Third, pigments such as spots are deposited under the eyes, forming a dark circle. In addition, hormonal abnormalities, skin diseases, chronic fatigue, etc. can cause dark circles.

Recently, among the causes of dark circles, capillaries under the eyes are known to be most closely associated with dark circles. The blood pressure of the capillaries located under the eye rises, which causes the capillaries to rupture, thereby increasing vascular permeability, which causes the blood to accumulate and break down hemoglobin, a component of blood, to dark brown bilirubin ( As bilirubin is formed, dark circles are formed.

In addition, capillary blood pressure is known to be closely related to angiotensin II, which is a vasoconstrictor, and angiotensin II is converted from angiotensin I by angiotensin converting enzyme. Therefore, in order to alleviate the dark circle, development to inhibit the activity of angiotensin I converting enzyme is required.

On the other hand, cosmetics that are currently commercially available as a method of removing dark circles suppress the pigmentation of the skin or focus on exfoliation and it is difficult to expect the effect.

Accordingly, the present inventors have tried to develop a cosmetic composition that can alleviate the dark circle, and as a result, the extract extracted from horse chestnut tree was found to have an excellent effect on inhibiting the production of angiotensin II by inhibiting angiotensin I converting enzyme to complete the present invention. . Accordingly, it is an object of the present invention to provide a cosmetic composition for relaxing dark circles containing horse chestnut extract.

The present invention for solving the above problems may include the following means.

Dark circle relief cosmetic composition according to the invention is characterized in that it contains the horse chestnut extract as an active ingredient.

On the other hand, the horse chestnut extract is characterized in that it contains 0.001% to 30.0% by weight relative to the total weight of the cosmetic composition.

Angiotensin I-converting enzyme inhibition cosmetic composition according to the present invention contains the horse chestnut extract as an active ingredient.

Capillary blood fortification and blood circulation promoting cosmetic composition according to the present invention contains a chinensis extract as an active ingredient.

The cosmetic composition is characterized in that it has a formulation selected from the group consisting of softening cream, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder.

The horse chestnut extract of the present invention has an inhibitory effect of angiotensin I converting enzyme, and also has an effect of reducing capillary permeability by strengthening capillaries, and when applied to the cosmetic of the present invention, it promotes blood circulation of the skin. It can provide the effect of mitigating dark circles.

Hereinafter, the present invention will be described in detail.

The cosmetic composition according to the present invention contains the horse chestnut extract to improve the dark circle.

Horse chestnut ( Aesculus turbinata Blume ) used in the present invention belongs to the Hippocastanaceae , the horse chestnut extract of the present invention refers to those obtained from the leaves or seeds. Horse chestnut is a plant native to Japan and native to the sub-central region of Korea. It is cultivated for ornamental purposes, and is used for the treatment of skin diseases, frostbite, and pain in oriental medicine.

The horse chestnut extract included in the cosmetic composition of the present invention may be obtained by various extraction methods, and specifically may be obtained by the following extraction method.

As the extraction solvent, purified water, anhydrous alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol), acetone, ethyl acetate, glycerin, butylene glycol, a solvent of propylene glycol or a mixture thereof, most preferably 80% ethanol Put it in use. The ratio of the pulverized product and the extraction solvent is 1: 5 to 1:30, preferably 1:10. The extractor is extracted at 45 ° C.-100 ° C., most preferably at 50 ° C.-70 ° C. for 1 hour-48 hours, most preferably 3 hours-10 hours, and is obtained by vacuum concentration or freeze drying.

However, the extract of the present invention is not limited to the above extraction solvent, it will be apparent to those skilled in the art that a horse chestnut extract which can exhibit substantially the same effect even using other extraction solvents can be obtained.

On the other hand, the horse chestnut extract used in the present invention is suitable to contain 0.001 to 30.0% by weight based on the total weight of the cosmetic composition. Preferably it is 0.1-10.0 weight%. When the content of the horse chestnut extract is less than 0.001% by weight it is difficult to expect the effect, when it exceeds 30.0% by weight does not appear to increase the apparent effect of the increase in content.

The composition of the present invention may further contain at least one active ingredient exhibiting the same or similar function in addition to the horse chestnut extract.

Cosmetics prepared with a cosmetic composition containing the horse chestnut extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing horse chestnut extract can be prepared in the form of adjuvants that can be applied topically or systemically used commonly in the field of dermatology by containing a dermatologically acceptable medium or base.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to horse chestnut extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water Commonly used in ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatology field, such as any other ingredient. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Examples of products to which the extract of the present invention may be added include, for example, astringent cosmetics, softening cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and cosmetics such as cleansing agents, face washes, soaps, treatments, cosmetics, and the like. There is this.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Preparation Example 1 Preparation of Horse Chestnut Extract

1 kg of horse chestnut seeds are washed with clean water and dried in a cool shade. Thereafter, the dried horse chestnut seeds are finely pulverized using a grinder, and 500 g of the pulverized product is placed in an extractor equipped with a cooling capacitor, and 5 liters of 80% ethanol is added thereto, followed by heating at 70 ° C. for 10 hours. The extract was filtered with a 400 mesh filter cloth, and the filtrates were collected. The solvent was evaporated completely using a distillation apparatus equipped with a cooling condenser, and then concentrated to obtain 103 g of the extract.

Experimental Example 1 Measurement of Angiotensin Converting Enzyme Inhibition Rate of Seven Leaf Extracts

In order to measure the inhibitory effect of the angiotensin converting enzyme using the horse chestnut extract obtained in Preparation Example 1 was carried out according to the method of Cuchiman and cheung [Cushman, D.W. and Cheung, H. S., Biochem. Pharmacol., 20, 1637, 1971].

50 μl of 0.5%, 1.0% and 2.0% of the sample was dissolved in distilled water, and then 50 μl of hippuryl-his-leu solution (25 mg / 2.33 mL sodium borate buffer, pH 8.3) and sodium borate buffer were used as substrates. 100 μl of pH 8.3) was added and pre-reacted at 37 ° C. for 10 minutes. 50 µl of angiotensin I-converting enzyme solution was added thereto, followed by further reaction at 37 ° C for 30 minutes, and then 250 µl of 1N hydrochloric acid was added to stop the reaction. The control group was added 50 µl of distilled water instead of the sample solution. After the reaction was completed, 1.5 ml of ethyl acetate was added, shaken for 15 seconds, mixed well, and 1.25 ml of the supernatant was taken. The supernatant was completely dried at 105 ° C. for 1 hour, dissolved in 1.5 ml of distilled water, and absorbance was measured at 228 nm to obtain angiotensin I converting enzyme inhibitory effect.

Sample concentration (%) Enzyme inhibitory effect (%) 0.5 19.5 1.0 49.8 2.0 80.4 Control 0.0

As can be seen from the results of Table 1, the angiotensin I converting enzyme inhibitory effect was increased as the concentration of the sample was increased. Therefore, the production of angiotensin II was reduced, capillary contraction was suppressed, blood pressure was decreased, and capillary was increased. The antipermeable effect of blood vessels can be expected.

Experimental Example 2 Capillary Permeability Inhibitory Effect of the Seven Leaf Extracts

In order to determine the capillary permeability inhibitory effect of the horse chestnut extract obtained in Preparation Example 1 was tested as follows.

Cultured pulmonary endothelial cell line (CPAE) cell lines were cultured under Dulbecco's modified Eagles medium (DMEM) medium containing 10% fetal bovine serum under 37 ° C. and 5% CO 2, followed by 2 × 10 ⁵ cells in a 12-well plate. (Costar Transwell Clear, # 3460, tissue culture-polyester membrane, pore size 0.4㎛) and incubated for 4 days. The plate used for the parent test consists of the upper luminal compartment and the lower luminal compartment. The reagent used for the test is Evans blue (EB), Sigma, final concentration 90 [Mu] g / ml) and bovine serum albumin (BSA), Sigma (final concentration 40 mg / ml) were dissolved in Hank's buffered saline solution (HBSS, pH 7.4) and added together to the upper lumen compartment. In the lower luminal compartment, 1.5 ml of HBSS was placed, and at regular intervals, 75 μl of the lower luminal compartment was taken into a 96-well plate and absorbance was measured at 680 nm.

The test substance was added to the upper lumen compartment, and the effect of inhibiting vascular permeability was measured by treating EB-BSA solution containing H ₂ O ₂ and test substance simultaneously. As a control, EB-BSA solution to which 1 mM H 2 O 2 was added was used, and the results of capillary permeability inhibitory effect are shown in Table 2 below.

Sample concentration (%) Capillary permeation inhibition effect (%) 0.005 10.9 0.010 28.4 0.020 40.6 Control 0.0

As can be seen from the results of Table 2, as the concentration of the sample was increased, it was confirmed that capillary permeability was suppressed. Therefore, it can be expected to have an effect of promoting blood circulation.

[Production Example 2]

Table 3 below was prepared in the form of nutrition cream having a composition ratio, the cosmetic composition containing the horse chestnut extract obtained in Preparation Example 1 as Examples 1, 2, the cosmetic composition containing no horse chestnut extract as a comparative example It was.

ingredient Example 1 (% by weight) Example 2 (% by weight) Comparative Example (wt%) Horse chestnut extract 10.0 5.0 glycerin 5.0 5.0 5.0 Liquid paraffin 10.0 10.0 10.0 Polysorbate 60 2.0 2.0 2.0 Sorbitan sesquirololeate 1.0 1.0 1.0 Squalane 5.0 5.0 5.0 Beeswax 10.0 10.0 10.0 Triethanolamine 0.5 0.5 0.5 Propylene glycol 1.0 1.0 1.0 Carboxyl Vinyl Polymer 1.5 1.5 1.5 incense a very small amount a very small amount a very small amount antiseptic a very small amount a very small amount a very small amount Purified water Balance Balance Balance system 100 100 100

Experimental Example 3 Evaluation of Blood Circulation Promoting Effect Using LDPI

In order to investigate the effect of promoting blood circulation of the skin according to the decrease in permeability of capillaries, the following experiment was performed.

Laser Doppler Perfusion Imager (LDPI) was used to evaluate the effect of promoting blood circulation in the skin of the compositions of Examples 1, 2 and Comparative Examples of the present invention prepared in Preparation Example 2. LDPI uses a low-energy laser of 630nm as a light source to scan the skin and measure the light reflected back. This measure is widely used as a device for measuring blood circulation in the skin because it is proportional to the blood flow of the skin. Twenty females in their 20s and 30s were tested for blood circulation promoting effects. In the constant temperature and humidity room where the LDPI is located, the forearm of the arm, which is the test site, was wiped with ethanol and dried, and then the initial value of the test site was measured using LDPI, and blood flow was measured again 1 hour after the application of the sample. The average amount of blood change was 5 sites per arm. The results are shown in Table 4 below, and the results are expressed as an increase rate relative to the initial value.

Cosmetics Blood flow increase rate (%) Example 1 9.4 Example 2 4.4 Comparative example 0.2

As can be seen from the results of Table 4, it was found that the blood flow increased in a concentration-dependent manner in Examples 1 and 2 containing the horse chestnut extract, and thus the effect of promoting blood circulation was found by the active ingredient of the example.

Experimental Example 4 Dark Circle Relaxation Effect

In order to examine the skin color brightness effects of Examples 1 and 2 and Comparative Examples, 30 healthy females in their 30s and 40s with dark circles were divided into three groups of 10 people. After applying twice a day for 4 weeks, the change in skin color was measured using Mexameter (Courage + Khazaka GmbH, Germany). The results are shown in Table 5 below.

sample Skin color change (△ L) 4 Weeks Example 1 9.7 Example 2 4.2 Control 0.4

As can be seen from Table 5, Examples 1 and 2 containing the horse chestnut extract showed a change in the brightness of the skin color.

Hereinafter, as an example of the cosmetic formulation of the present invention, the softening lotion, lotion, essence, massage cream, shampoo and body cleanser containing the active ingredient of the present invention are exemplified, but the formulation of the cosmetic of the present invention is not necessarily limited thereto. .

Formulation Example 1 Flexible Cosmetics

Softening makeover containing horse chestnut extract was prepared according to a conventional method.

Raw material name Content (% by weight) Horse chestnut extract 10.0 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

[Formulation Example 2] Lotion

Lotions containing horse chestnut extract were prepared according to conventional methods.

Raw material name Content (% by weight) Horse chestnut extract 10.0 Cetostearyl alcohol 1.0 Glyceryl Monostearate 0.8 Sorbitan monostearate 0.3 Porofilparaben 0.1 Polysorbate 60 1.0 Mineral oil 5.0 Pyromethicone 3.0 Dimethicone 0.5 Allantoin 0.1 glycerin 5.0 Alcohol 2 Propylene glycol 3.0 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 3 Essence

Essences containing horse chestnut extract were prepared according to a conventional method.

Raw material name Content (% by weight) Horse chestnut extract 10.0 1,3-butylene glycol 4.0 glycerin 3.0 Allantoin 0.5 Panthenol 0.1 EDTA-2Na 0.01 ethanol 5.0 Triethanolamine 1.5 Squalane 2.0 Beeswax 2.5 Polysorbate 60 3.5 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.5 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 4 Massage Cream

Massage cream containing horse chestnut extract was prepared according to a conventional method.

Raw material name Content (% by weight) Horse chestnut extract 10.0 glycerin 4.0 Vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 5 Shampoo

Shampoo containing horse chestnut extract was prepared according to a conventional method.

Raw material name Content (% by weight) Horse chestnut extract 10.0 Alkyl ether sulfate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 3.0 Lauramid 4.0 EDTA-2Na 0.1 Polyquaternium-10 0.05 NaCl 0.1 Citric acid 2.4 incense a very small amount antiseptic a very small amount Purified water Balance system 100

Formulation Example 6 Body Cleanser

Body cleanser containing horse chestnut extract was prepared according to a conventional method.

Raw material name Content (% by weight) Horse chestnut extract 10.0 Alkyl ether saccharide (30%) 30.0 Alkyl ether sulfosuccinate (30%) 20.0 Alkylamidobetaine (30%) 5.0 Propylene glycol 5.0 Betaine 5.0 Lauramiddiei 4.0 Cellulose gum 0.05 Hydrolyzed protein 0.1 Citric acid a very small amount NaCl a very small amount incense a very small amount antiseptic a very small amount Purified water Balance system 100

Claims (5)

Dark circle relaxation cosmetic composition containing a horse chestnut extract as an active ingredient. According to claim 1, wherein the horse chestnut extract is dark circle relaxation cosmetic composition characterized in that it contains 0.001% to 30.0% by weight relative to the total weight of the cosmetic composition. Cosmetic composition for inhibiting angiotensin I-converting enzyme containing horse chestnut extract as an active ingredient. Cosmetic composition for strengthening capillary and promoting blood circulation containing the horse chestnut extract as an active ingredient. The cosmetic composition according to any one of claims 1 to 4, wherein the cosmetic composition is a softening lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray and powder Cosmetic composition, characterized in that it has a formulation selected from the group consisting of.