KR20100098807A - Annimal food comprising omega-3 fatic acid - Google Patents

Abstract

PURPOSE: A flaxseed oil additive composition for production of omega-3 fatty acid milk is provided to improve the content of the omega-3 fatty acid within a meat and to reduce the content of cholesterol by adding flaxseed oil water soluble powder and an extract. CONSTITUTION: A flaxseed oil additive composition is supplied to milk cow by being mixed in an amount of 0.1-50 weight% based on the total amount of feed. The flaxseed oil additive composition includes flaxseed oil water soluble powder or a flaxseed oil water soluble extract. The flaxseed soil additive water soluble powder contains a large amount of omega-3 fatty acid which is manufactured by mixing water with 1-99 weight% of flaxseed oil including linolenic acid, eicosapentaenoic acid, docosahexaenoic acid, 30-90 weight% of epigallocatechin-3-galate, 6-35 weight% of epigallocatechin, and 1-99 weight% of a green tea catechin extract containing the epicatechin-3-gallate.

Description

Flaxseed oil additive composition for milk production of omega-3 fatty acid {annimal food comprising omega-3 fatic acid}

The present invention relates to a method of preparing a feed additive for producing milk having a high content of unsaturated fatty acids, and more particularly, to a cow fed a flaxseed oil additive composition for producing omega-3 fatty acid milk to cows in comparison with ordinary milk. It relates to a flaxseed oil additive composition for the production of omega-3 fatty acid milk containing a large amount of omega-3 fatty acids to produce omega-3 fatty acid milk containing 2 to 10 times the amount of three fatty acids to increase the content of omega fatty acids and lower cholesterol content.

As the consumption of livestock products such as meat, eggs, and milk increases rapidly due to the increase of national income, fat intake also increases, and it causes various adult diseases such as high blood pressure and heart disease by increasing the amount of excess fatty acid cholesterol in the diet. In particular, it is well known that animal fats have a higher degree of cholesterol deposition in the body than most vegetable fats, most of which are saturated fatty acids.

On the other hand, omega fatty acids, which are known to be high in oils of blue fish, have been known to relieve the deposition of cholesterol in the body to prevent heart disease and high blood pressure. Especially, eicoshexahexaenoic acid (aka EPA) and docosahexa Enoic acid (also known as DHA) is a fatty acid closely related to brain development with 20 and 22 carbon atoms and high unsaturation, respectively, and has recently been used as an additive in various foods.

In general, since fats are liquid and solid at room temperature according to their physical properties, dehydrated soap powders and fat powders for reducing automatic oxidation have been proposed to solve handling inconveniences. Known. In the cold spray dry method, the fat is first solidified in an air blast chamber at 5 to -30 ° C by adding an emulsifier and a protein carrier, followed by clumping. In order to prevent the mixture, it is prepared by mixing with starch, which is a powdered diluent, or by forming an emulsion solution with an emulsifier, water skim milk powder, etc., and spray drying in air at 120 to 170 ° C. In addition, the fatty acid is hydrolyzed with a strong acid or strong alkali and then immersed in a salt of the fatty acid by adding a metal salt, washed with an alkali and dried to obtain a fatty acid powder. Products made by the above-described methods are easy to handle, but in the case of products made with fatty acid salts, the chemical properties of fats are triglyceride, ester form, free fatty acid ( free fatty acid), since the oxidation rate is greatly increased as the metal salt of the fatty acid proceeds, the fatty acid of the product made of free fatty acid is mainly composed of 90 to 80% saturated fatty acid.

These conventional fat feed additives increase the content of omega-3 unsaturated fatty acids and cannot produce pork with low cholesterol. In the case of fatty acid products, in order to have a high melting point when used as livestock feed, and to absorb and absorb these products, the metal components (Ca, Na) bound by the acidity of the stomach must be freed from fatty acids quickly. However, there is a problem that the actual absorption of the small intestine (intestine) of the livestock is not so good.

The present invention solves the above problems and can produce a ring of high omega fatty acid content and low cholesterol content produced by using as an additive for the production of omega-3 fatty acid milk, rich in omega fatty acid in the fatty acid composition of animal foods and cholesterol An additive is provided for producing low content meat. In addition, the additives for producing omega-3 fatty acid milk of the present invention are used in omega-3 fatty acids, ie, linolenic acid and eicosapenta in meat produced by maintaining a constant nutrient absorption rate when feeding an emulsion solution of vegetable fatty acid to livestock. Increasing the content of Eicosapen aenoic acid and Docosahexaenoic acid and reducing the cholesterol content.

The present invention is to prepare a flaxseed oil water-soluble powder and extract for omega-3 fatty acid milk production containing a large amount of omega-3 fatty acids mixed with flaxseed oil and green tea extract and water-soluble flaxseed oil for omega-3 fatty acid milk production of the present invention Powders and extracts are added to the feed to increase the content of omega-3 fatty acids, linolenic acid, eicosapen aenoic acid and docosahexaenoic acid, and cholesterol in the feed. The present invention relates to a flaxseed oil-soluble powder and extract for producing omega-3 fatty acid milk containing a large amount of omega-3 fatty acids which can reduce the content of (Cholesterol).

The present invention is an omega-3 fatty acid linolenic acid (linolenic acid), eicosapentaenoic acid (Eicosapen) by adding a linseed oil water-soluble powder and extract for feed containing a large amount of omega-3 fatty acids mixed with linseed oil and green tea extract It is very useful for livestock industry because it can increase aenoic acid) and docosahexaenoic acid content and reduce cholesterol content.

The present invention is 1 to 99% by weight flaxseed oil containing 1 to 99% by weight of linolenic acid, Eicosapen aenoic acid and docosahexaenoic acid and 30 to 90 Wt% epigallocatechin-3-gallate (EGCG, C ₂₂ H ₁₈ O ₁₁ , molecular weight 458.4), 6-35 wt% epigallocatechin (EGC, C ₁₅ H ₁₄ O ₇ , molecular weight 306.3) and Omega-3 fatty acid prepared by mixing 1 to 99% by weight of green tea catechin extract containing 2 to 35% by weight of epicatechin-3-gallate (ECG, C ₂₂ H ₁₈ O ₁₀ , molecular weight 442.5) Flaxseed oil-soluble powder containing a large amount; 1 to 99% by weight of flaxseed oil and 1 to 99% by weight of linolenic acid, Eicosapen aenoic acid and 1 to 99% by weight of docosahexaenoic acid and 30 to 90% by weight. Epigallocatechin-3-gallate (EGCG, C ₂₂ H ₁₈ O ₁₁ , molecular weight 458.4), 6 to 35 wt% epigallocatechin (EGC, C ₁₅ H ₁₄ O ₇ , molecular weight 306.3) and 2 to Flaxseed containing a large amount of omega-3 fatty acid prepared by mixing 1 to 99% by weight of green tea catechin extract containing 35% by weight of epicatechin-3-gallate (ECG, C ₂₂ H ₁₈ O ₁₀ , molecular weight 442.5) in water Omega-3 milk production, characterized in that to produce any omega-3 fatty acid milk containing 2 to 100 times the amount of omega-3 fatty acids compared to normal milk by feeding the cow any one composition selected from the milk-soluble extracts The present invention relates to a flaxseed oil additive composition.

In the present invention, the flaxseed oil additive composition is fed to the cow mixed with 0.1 to 50% by weight relative to the total amount of feed to be fed omega-3 fatty acid containing 2 to 100 times the amount of omega-3 fatty acids compared to normal milk It is characterized by producing milk. In the flaxseed oil additive composition for omega-3 milk production of the present invention, the flaxseed oil additive composition is fed 0.05 to 50% by weight relative to the total amount of clean water to be fed to cows to provide omega-3 fatty acids in comparison with normal milk. It is characterized by producing an omega-3 fatty acid milk containing 2 to 100 times the amount. In the flaxseed oil additive composition for producing omega-3 milk of the present invention, the green tea catechin extract is characterized in that any one selected from green tea catechin extract powder and 10 to 90% by weight green tea catechin extract extract. In the flaxseed oil additive composition for producing omega-3 fatty acid milk of the present invention, the flaxseed oil-soluble powder is flaxseed oil using any one coating agent selected from glucose, galactose, ribose, maltose, sugar, lactose, starch, glycogen and cellulose. It is characterized in that it is prepared by coating 10 to 90% by weight based on the total amount of green tea catechin extract and coating agent. In the flaxseed oil additive composition for producing omega-3 fatty acid milk of the present invention, the flaxseed oil water-soluble extract is prepared by coating 1 to 10% by weight based on the total amount of flaxseed oil, green tea catechin extract and coating agent using a water-soluble emulsifier. It is characterized by.

Example 1 Obtaining Green Tea Catechin Extract Used in Flaxseed Oil Water-Soluble Powder for Omega-3 Fatty Acid Milk Production Containing Large Omega-3 Fatty Acids

1000 g of green tea (Theae sinensis L., Theaceae-var.bohea nayabugida, Jeju Island) was extracted with 10 l of 70% (v / v) ethanol solution for 3 hours at 85 to 90 ° C, and then the same conditions were applied with 5 l of the same solvent. After completion of the second extraction, it was concentrated in a vacuum rotary evaporator to about 1000 mL. Water was added to the concentrate, diluted 2-3 times, centrifuged at 14,000 rpm for 20 minutes to completely remove solids, and the collected supernatants were extracted twice with 1.2-fold chloroform. Then, the chloroform layer was removed, the aqueous layer was extracted four times with 1.2 times ethyl acetate, and the obtained solution was combined, and dried under reduced pressure until the extraction solvent disappeared completely. The dried product was dissolved in a small amount of water to obtain an extract of green tea catechin.

Example 2 Obtaining Green Tea Catechin Extract Used in Flaxseed Oil Water-Soluble Powder for Milk Production of Omega-3 Fatty Acids Containing Large Omega-3 Fatty Acids

1000 g of green tea (Theae sinensis L., Theaceae-var.bohea yabugida, Jeju Island) was extracted with 10 l of 70% (v / v) ethanol solution for 3 hours at 85 to 90 ° C, and then under the same conditions with 5 l of the same solvent. The secondary extraction was completed and concentrated to about 1000 ml in a vacuum evaporator. Water was added to the concentrate, diluted 2-3 times, centrifuged at 14,000 rpm for 20 minutes to completely remove solids, and the collected supernatants were extracted twice with 1.2-fold chloroform. Then, the chloroform layer was removed, the aqueous layer was extracted four times with 1.2 times ethyl acetate, and the obtained solution was combined, and dried under reduced pressure until the extraction solvent disappeared completely. The dried product was dissolved in a small amount of water and lyophilized to obtain a powdered green tea catechin extract.

In addition, as a result of measuring the content of the catechin contained in the powdered green tea catechin extract obtained by a known method (see Korean Patent Publication No. 2001-84165), with respect to the weight of the obtained green tea catechin powder, epi Gallocatechin-3-gallate 37.5% (w / w), epigallocatechin 12.5% (w / w), epicatechin-3-gallate 5.7% (w / w) and the balance of other catechins Could know.

Green tea is obtained by processing young leaves of the tea tree [Thae sinensis L. (Theaceae)] in early spring, and it has been reported that the green tea components exhibit various biological activities. As a representative example, anti-cancer effect, antioxidant effect, antimutagenic effect, skin protection effect, neuroprotective effect, anti-inflammatory effect, cell induction, etc., have been reported using green tea extract and components separated from green tea, and catechins of green tea Has been reported to have anticancer and antioxidant effects. In addition, epigallocatechin-3-gallate (EGCG) and (-)-epigallocatechin (EGC), known as the main active ingredients of green tea, have anti-cancer effects, antioxidant effects, and skin protection. It has been reported that there is an effect, a cell death effect, polyphenol (green tea) also has anti-inflammatory effect, cancer prevention effect, antioxidant effect, brain cell protection effect.

Example 3 Preparation of Flaxseed Oil Water-Soluble Powder for Milk Production of Omega-3 Fatty Acids Containing Large Omega-3 Fatty Acids

10% by weight flaxseed oil containing 55.12% by weight linolenic acid, Eicosapen aenoic acid and docosahexaenoic acid and epigallocatechin-3-gallate ( EGCG, C ₂₂ H ₁₈ O ₁₁ , molecular weight 458.4), epigallocatechin (EGC, C ₁₅ H ₁₄ O ₇ , molecular weight 306.3) and epicatechin-3-gallate (ECG, C ₂₂ H ₁₈ O ₁₀ , molecular weight 442.5) 20% by weight of green tea catechin extract extract containing 70% by weight of 30% of water is mixed with 70% by weight of glucose while heating, and is coated at high speed at 3000rpm to provide a large amount of omega-3 fatty acids. A flaxseed oil soluble powder for -3 fatty acid milk production was prepared.

Example 4 Preparation of Flaxseed Oil Water-Soluble Powder for Milk Production of Omega-3 Fatty Acids Containing Large Omega-3 Fatty Acids

5 g of an emulsifier (Twen 60: Tween 60) was added to 10 g of flaxseed oil and 20 g of green tea catechin extract extract, and the mixture was heated and heated to 50 ° C., followed by mixing 70 g of glucose with the mixture, obtaining an emulsion with a homogenizer, followed by a fluidized bed dryer ( After mixing the seed material in a fluid bed dryer and flowing the seed material with the inlet air adjusted to 50 ° C., the emulsion solution is spray-dried at the upper part of the fluidized bed dryer to spray omega-3 fatty acid. A flaxseed oil soluble powder for -3 fatty acid milk production was prepared. Analysis of the powder showed that the linseed oil-soluble powder contained 55.12% omega-3 fatty acids.

Example 5 Preparation of Flaxseed Oil Water-Soluble Extract for Production of Omega-3 Fatty Acid Milk Containing a Large Omega-3 Fatty Acid

50% by weight flaxseed oil and 55% by weight linolenic acid, Eicosapen aenoic acid and Docosahexaenoic acid and epigallocatechin-3-gallate EGCG, C ₂₂ H ₁₈ O ₁₁ , molecular weight 458.4), epigallocatechin (EGC, C ₁₅ H ₁₄ O ₇ , molecular weight 306.3) and epicatechin-3-gallate (ECG, C ₂₂ H ₁₈ O ₁₀ , molecular weight 442.5) 20% by weight of the green tea catechin extract extract (containing 70% by weight of 30% water) was mixed with 25% by weight of water, and 5 g of an emulsifier (Twen 60: Tween 60) was added and coated with high speed at 3000 rpm. A flaxseed oil-soluble extract for producing omega-3 fatty acid milk containing a large amount of 3 fatty acids was prepared.

Example 6 Preparation of Flaxseed Oil Additive Composition for Omega-3 Fatty Acid Milk Production

50 g of flaxseed oil and 20 g of green tea catechin extract extract (70% by weight containing 30% of water) are mixed with 25 g of water, and then 5 g of an emulsifier (Twen 60: Tween 60) is added and heated to 50 ° C. with a high speed homogenizer at 3000 rpm. Coating with rotation to prepare a flaxseed oil water-soluble extract for producing omega-3 fatty acid milk containing a large amount of omega-3 fatty acids. As a result of analyzing the extract, 54.77% of omega-3 fatty acids were contained in the flaxseed oil-soluble extract.

Example 7 A method of producing omega-3 fatty acid milk containing a large amount of omega-3 fatty acid

50 g of flaxseed oil and 20 g of green tea catechin extract extract (70% by weight containing 30% of water) are mixed with 25 g of water, and then 5 g of an emulsifier (Twen 60: Tween 60) is added and heated at 50 ° C. with a homogenizer at 3000 rpm. Coating with rotation to prepare a flaxseed oil water-soluble extract containing a large amount of omega-3 fatty acids. When the composition is fed to cows by diluting clean water 1000 times with the flaxseed oil water-soluble extract, the cow's body can be efficiently absorbed without physiological sequelae, and rapidly decreases n-6 fatty acids in milk and a large amount of n-3. Fatty acids have accumulated.

To compare the fatty acid composition present in the milk produced by the composition for milk production of the present invention, 40 cows were divided into two groups and the first group (control group) was supplied with commercially available milk production feed, and the second The group (experimental group) was fed the milk production composition of this invention for 30 days, respectively. The proportion of milk produced in each group was measured and the average value was compared.

The composition ratio of n-6 / n-3 fatty acids in milk produced by commercial feed (control) and cows raised with the milk production composition (experimental group) of the present invention was investigated. In the milk produced from commercial feed-fed cows (control), the n-6 / n-3 fatty acid ratio was 11.8, which was significantly higher in n-6 fatty acids, but in cows fed the milk-producing composition of the present invention (experimental group). The ratio of n-6 / n-3 fatty acids in milk produced increased sharply from day 3 after n-3 fatty acids began to increase, and from day 10 the ratio of n-6 / n-3 fatty acids began to increase. As this 1.3, n-3 fatty acids increased greatly and showed the same value continuously over time. Only 10 days after feeding the cow composition of the present invention to the cow was able to obtain a very high milk ratio of n-3 fatty acids. Accumulated milk with a large amount of n-3 fatty acids, the ratio of n-6 / n-3 fatty acids to 1.3, has not been successful in any studies yet, and the highest level of n-3 fatty acids produced so far has been achieved. Contains milk.

Milk produced by feeding the composition for milk production of the present invention to cows has a low content of n-6 fatty acids and a large amount of n-3 fatty acids are accumulated. It does not occur, it is possible to prevent various physiological diseases due to excessive intake of n-6 fatty acids and to maintain the physiological balance in the body by reducing the ratio of n-6 / n-3 fatty acids. Therefore, until now, there was a tendency to avoid milk because of the recognition that excessive saturated fatty acids or n-6 fatty acids are present in the milk, but the milk produced by feeding the milk composition for milk production of the present invention to cows has reduced n-6 fatty acid content. On the other hand, the amount of n-3 fatty acids has been greatly increased, allowing much more milk to be consumed than ever before.

In addition, in order to measure the degree of transition of unsaturated fatty acid into milk in the milk fed the feed additive of the present invention was performed in vivo using a cow. That is, the feed additives were mixed in the cow's feed and fed with milk 250g per day for 28 days, milk was collected at 1, 2, and 4 weeks to analyze fatty acids. The results of the analysis showed that the milk containing the feed additives contained a large amount of EPA and DHA, and the ratio of saturated fatty acids was significantly reduced in the ratio of saturated fatty acids and unsaturated fatty acids in the milk.

The feed additive prepared according to the method of the present invention transfers polyunsaturated fatty acids such as DHA and EPA to milk with high efficiency to prevent various adult diseases such as hypertension and heart disease, and the feed additive is not decomposed by microorganisms in the cow's first place. It is bypassed to allow highly polyunsaturated fatty acids to be transferred to the final product milk with high efficiency.

The feed additive of the present invention has a very excellent effect in producing milk with a high content of polyunsaturated fatty acids and a significantly reduced content of saturated fatty acids, and the present invention provides a method of preparing such feed additives to improve the health of national health. It will contribute to the improvement and prevention of adult diseases.

1 is a food test report for the omega-3 fatty acid milk fed the composition of the present invention

Figure 2 is a food test report for the omega-3 fatty acid milk fed the composition of the present invention

Claims (6)

1 to 99% by weight of flaxseed oil and 30 to 90% by weight of linolenic acid, Eicosapen aenoic acid and 1 to 99% by weight of Docosahexaenoic acid. Epigallocatechin-3-gallate (EGCG, C ₂₂ H ₁₈ O ₁₁ , molecular weight 458.4), 6 to 35 weight percent epigallocatechin (EGC, C ₁₅ H ₁₄ O ₇ , molecular weight 306.3) and 2 to 35 Flaxseed containing a large amount of omega-3 fatty acids prepared by mixing 1 to 99% by weight of green tea catechin extract containing 1% by weight of epicatechin-3-gallate (ECG, C ₂₂ H ₁₈ O ₁₀ , molecular weight 442.5) Oil soluble powder; 1 to 99% by weight of flaxseed oil and 1 to 99% by weight of linolenic acid, Eicosapen aenoic acid and 1 to 99% by weight of docosahexaenoic acid and 30 to 90% by weight. Epigallocatechin-3-gallate (EGCG, C ₂₂ H ₁₈ O ₁₁ , molecular weight 458.4), 6 to 35 wt% epigallocatechin (EGC, C ₁₅ H ₁₄ O ₇ , molecular weight 306.3) and 2 to Flaxseed containing a large amount of omega-3 fatty acid prepared by mixing 1 to 99% by weight of green tea catechin extract containing 35% by weight of epicatechin-3-gallate (ECG, C ₂₂ H ₁₈ O ₁₀ , molecular weight 442.5) in water Omega-3 milk production, characterized in that to produce any omega-3 fatty acid milk containing 2 to 100 times the amount of omega-3 fatty acids compared to normal milk by feeding the cow any one composition selected from the milk-soluble extracts Flaxseed oil additive composition. The method according to claim 1, wherein the flaxseed oil additive composition is fed to the cow mixed with 0.1 to 50% by weight relative to the total amount of feed to feed omega-3 fatty acids containing 2 to 100 times the amount of omega-3 fatty acids compared to normal milk -3 Flaxseed oil additive composition for omega-3 milk production, characterized by producing fatty milk. According to claim 1, The flaxseed oil additive composition is fed to the cow mixed with 0.05 to 50% by weight relative to the total amount of clean water to be fed omega-3 fatty acids containing 2 to 100 times the amount of omega-3 fatty acids compared to normal milk -3 Flaxseed oil additive composition for omega-3 milk production, characterized by producing fatty milk. The flaxseed oil additive composition for producing omega-3 fatty acid milk according to claim 1, wherein the green tea catechin extract is any one selected from green tea catechin extract powder and 10-90 wt% green tea catechin extract extract. According to claim 1, The flaxseed oil water-soluble powder is based on the total amount of flaxseed oil, green tea catechin extract and coating agent using any one coating agent selected from glucose, galactose, ribose, maltose, sugar, lactose, starch, glycogen and cellulose. Flaxseed oil additive composition for producing omega-3 fatty acid milk, characterized in that it is prepared by coating 10 to 90% by weight. The omega-3 milk of claim 1, wherein the flaxseed oil water-soluble extract is prepared by coating 1 to 10% by weight based on the total amount of flaxseed oil, green tea catechin extract and coating agent using a water-soluble emulsifier. Flaxseed oil additive composition for production.

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