KR100932478B1 - Annimal food comprising omega-3 fatic acid - Google Patents

Abstract

PURPOSE: A feed additive composition, and a method for producing the milk, chicken or beef containing omega-3 fatty acid are provided to increase the content of omega-3 fatty acid and to lower the content of cholesterol. CONSTITUTION: A feed additive composition is prepared by mixing 50~90 weight% of an flaxseed oil and 10~50 weight% of a green tea extract; and adding 5~20 weight% of and an edible emulsifier to the mixture. The flaxseed oil is prepared by heating flaxseed at 80~120°C for 10~40 min to remove moisture from the flaxseed and rapidly cooling it; extracting an oil from the cooled flaxseed at 80~120°C and filtering it with 200 mesh; and naturally precipitating the filtered flaxseed oil for 10~20 days and collecting the upper part.

Description

Food additive composition containing water-soluble flaxseed oil from which malodor and cyanide (-CN) toxicity have been removed to produce products containing omega-3 fatty acids {annimal food comprising omega-3 fatic acid}

The present invention relates to a water-soluble flaxseed oil-containing feed additive composition in which malodor and cyanide (-CN) toxicity are removed to produce a product containing a large amount of omega-3 fatty acids, and more particularly, by feeding feed to livestock The present invention relates to a water-soluble linseed oil-containing feed additive composition in which malodor and cyanide (-CN) toxicity have been removed in order to produce a product containing a large amount of omega-3 fatty acids by increasing the content of omega fatty acids and lowering the cholesterol content in meat.

In the related art, in general, fat is in a liquid and solid state according to its physical properties at room temperature, so dehydrated soap powder and fat powder for reducing automatic oxidation to solve the inconvenience of handling. How to make is known. In the cold spray dry method, the fat is first solidified in an air blast chamber at 5 to -30 ° C by adding an emulsifier and a protein carrier, followed by clumping. In order to prevent the mixture, it is prepared by mixing with starch, which is a powdery diluent, or by forming an emulsifying solution with an emulsifier, water skim milk powder, etc., and spray-dried in air at 120 to 170 ° C. In addition, the fatty acid is hydrolyzed with a strong acid or strong alkali, immersed in a salt of a fatty acid by adding a metal salt, washed with an alkali and dried to obtain a fatty acid powder. Products made by the above described methods are easy to handle, but in the case of products made with fatty acid salts, the chemical state of fat proceeds to the state of triglycerides, ester foams, free fatty acids, and metal salts of fatty acids. Since the oxidation rate increases as much as possible, the fatty acid of the product made of free fatty acid is 90 to 80% of the saturated fatty acid.

These conventional fat feed additives increase the content of omega-3 unsaturated fatty acids and cannot produce pork with low cholesterol. In the case of fatty acid products, in order to have a high melting point when used as livestock feed, and to absorb and absorb these products, the metal components (Ca, Na) bound by the acidity of the stomach must be freed from fatty acids quickly. However, there is a problem that the actual absorption of the small intestine (intestine) of the livestock is not so good.

Conventional flaxseed oil cannot be used for food and feed because it has a bad smell like rotten egg or odor, and it is rancid when exposed to air for a long time, and it is difficult to digest when used for food and feed and has a very bad smell. There was this. The conventional flaxseed oil has a problem that it is not good taste and difficult to digest, so that the prepared food becomes wet when stored for a long period of time, causing rancidity in the air, and not having a crunchy taste.

The present invention solves the above problems and can produce a ring with a high content of omega fatty acids and a low cholesterol content in meat produced by using the feed additives of livestock, rich in omega fatty acids and cholesterol content in the fatty acid composition of animal foods Provide feed additives for low meat production. In addition, the feed additive of the present invention is the omega-3 fatty acids in the meat produced by maintaining a constant nutrient absorption rate when feeding the emulsion of vegetable fatty acids to livestock, that is, linolenic acid (linolenic acid), Eicosapentaenoic acid (Eicosapen aenoic acid ) And docosahexaenoic acid to increase the content of cholesterol (Cholesterol).

The present invention is to add a water-soluble flaxseed oil-containing feed additive composition to remove the odor and cyanide (-CN) toxicity in the feed to produce a product containing a large amount of omega-3 fatty acids are the omega-3 fatty acids contained in the feed Feed additives containing large amounts of omega-3 fatty acids that can increase linolenic acid, eicosapen aenoic acid and docosahexaenoic acid and reduce cholesterol content For flaxseed oil soluble powders and extracts.

The present invention is to add a water-soluble flaxseed oil-containing feed additive composition to remove the odor and cyanide (-CN) toxicity in the feed to produce a product containing a large amount of omega-3 fatty acids are the omega-3 fatty acids contained in the feed Linolenic acid, Eicosapen aenoic acid and docosahexaenoic acid can increase the content of cholesterol (Cholesterol) content is very useful in the livestock industry.

The present invention is a water-soluble flaxseed oil-containing feed additive composition in which odor and cyanide (-CN) toxicity are removed from chicken feed, pig feed, bovine feed, and fish feed, and flaxseed at 80-120 ° C. for 10-40 minutes. Heated to remove moisture from flaxseed and then rapidly cooled, put the fast-cooled flaxseed in the milking machine milked at the milking machine temperature of 80 ~ 120 ℃ and filtered in 200 mesh to obtain a filtered linseed oil, the filtered flaxseed oil 50 to 90% by weight of flaxseed oil from which malodor and cyanide (-CN) toxicity were removed by obtaining purified flaxseed oil through a process of spontaneous sedimentation for 10 to 20 days to recover the upper layer; And epigallocatechin-3-gallate (EGCG, C ₂₂ H ₁₈ O ₁₁ , molecular weight 458.4), epigallocatechin (EGC, C ₁₅ H ₁₄ O ₇ , molecular weight 306.3) and epicatechin-3-gallate (ECG Prepared by mixing 10 to 50% by weight of green tea extract containing C ₂₂ H ₁₈ O ₁₀ , molecular weight 442.5) and then adding an edible emulsifier corresponding to 5 to 20% by weight relative to the mixture of the flaxseed oil and green tea extract. A water-containing flaxseed oil-containing feed additive composition, wherein the water-soluble flaxseed oil-containing feed additive composition is added to any one feed selected from chicken feed, pig feed, bovine feed and fish feed to contain eggs containing a large amount of omega-3 fatty acids. Water-soluble flax with odor and cyanide (-CN) toxicity removed to produce products containing large amounts of omega-3 fatty acids, characterized by producing any of the products of milk, chicken, beef and fish Seed oil-containing feed additive composition.

In the present invention, the egg is 0.5% of the total amount of the feed to feed the laying hens water-soluble linseed oil-containing feed additive composition from which odor and cyanide (-CN) toxicity is removed to produce a product containing a large amount of omega-3 fatty acids To 10 to 10% by weight of the mixture to give the laying hens; And 0.05 to 10% by weight of the water-soluble flaxseed oil-containing feed additive composition, in which the malodor and cyanide (-CN) toxicity is removed, in order to produce a product containing a large amount of omega-3 fatty acids, relative to the total amount of clean water fed to laying hens. It is characterized in that to produce a omega-3 fatty acid egg containing 2 to 10 times the amount of omega-3 fatty acids in comparison to a normal egg by feeding to a laying hen in any one method selected from the method of feeding to the laying hens.

In the water-soluble linseed oil-containing feed additive composition in which malodor and cyanide (-CN) toxicity are removed to produce a product containing a large amount of omega-3 fatty acids of the present invention, milk produces a product containing a large amount of omega-3 fatty acids. To feed the cow by mixing 0.5 to 10% by weight of the water-soluble flaxseed oil-containing feed additive composition to remove the malodor and cyanide (-CN) toxicity to the total amount of the feed to the cow; And 0.05 to 10% by weight of the water-soluble flaxseed oil-containing feed additive composition, in which malodor and cyanide (-CN) toxicity is removed, to produce a product containing a large amount of omega-3 fatty acid, relative to the total amount of clean water fed to the cow. By feeding to the cow by any one method selected from the method of feeding to the cow, characterized in that to produce omega-3 fatty acid milk containing 2 to 10 times the amount of omega-3 fatty acids in comparison to normal milk.

In the water-soluble flaxseed oil-containing feed additive composition in which malodor and cyanide (-CN) toxicity have been removed to produce a product containing a large amount of omega-3 fatty acids of the present invention, chicken produces a product containing a large amount of omega-3 fatty acids. To feed the chicken by mixing 0.5 to 10% by weight of the water-soluble flaxseed oil-containing feed additive composition to remove the odor and cyanide (-CN) toxicity to the total amount of the feed to the chicken; And 0.05 to 10% by weight of the water-soluble flaxseed oil-containing feed additive composition, in which malodor and cyanide (-CN) toxicity is removed, to produce a product containing a large amount of omega-3 fatty acids, relative to the total amount of clean water fed to chickens. By feeding the chicken by any one of the methods selected from the method of feeding the chicken is characterized in that to produce omega-3 fatty acid chicken containing 2 to 10 times the amount of omega-3 fatty acids in comparison to the normal chicken.

In the present invention, the beef is prepared against the total amount of feed supplementing the cow with a water-soluble linseed oil-containing feed additive composition from which malodor and cyanide (-CN) toxicity are removed to produce a product containing a large amount of omega-3 fatty acids. A method of feeding cows by mixing 0.5 to 10% by weight; And 0.05 to 10% by weight of the water-soluble flaxseed oil-containing feed additive composition in which the malodor and cyanide (-CN) toxicity are removed to produce a product containing a large amount of omega-3 fatty acids, relative to the total amount of clean water fed to cows. By feeding to the cow in any one method selected from the method of feeding to the cow characterized in that the production of omega-3 fatty acid beef containing 2 to 10 times the amount of omega-3 fatty acids in comparison to the normal beef.

In the present invention, the production of flaxseed oil is heated for 10 to 40 minutes at 80 ~ 120 ℃ to remove moisture from the flaxseed and then rapidly cooled, put the fast cooled flaxseed in the milking machine and milked at the milking machine temperature 80 ~ 120 ℃ After filtering in 200 mesh to obtain a filtered flaxseed oil, and the purified flaxseed oil through a process of recovering the upper portion after natural precipitated the filtered flaxseed oil for 10 to 20 days to give a odor and cyanide (-CN) Prepares flaxseed oil which has been detoxified.
The production of flaxseed oil in the present invention can be heated for 10 to 40 minutes at 80 to 120 ℃ flaxseed to remove all the moisture from the linseed as well as the moisture contained in the flaxseed outer surface.
Unrefined flaxseed has a odor like gurin and has cyanide (-CN) toxicity, making it impossible to use for food. Only a small amount is used for medicinal purposes. Unrefined flaxseed has the same odor as grilled odor and contains cyanide (-CN) toxicity. However, among the flaxseed ingredients, odors such as odors of odor and cyanide (-CN) toxic components are volatile components that can be removed by heating and are chemical substances having a small chemical molecular weight. Therefore, when the crude flaxseed is heated at 80 to 120 ° C. for 10 to 40 minutes, a odor, such as a odor, may be odorous and components having cyanide (-CN) toxicity may be removed by heating.
In addition, when the crude flaxseed is heated at 80 to 120 ° C. for 10 to 40 minutes, the flaxseed itself may be rapidly cooled to prevent carbonization.
In the present invention, the production of flaxseed oil is heated for 10 to 40 minutes at 80 ~ 120 ℃ to remove all the moisture from the flaxseed as well as the moisture contained in the flaxseed surface, and then rapidly cooled, rapid Put the cooled flaxseed in the milking machine and milked at the milking machine temperature of 80 ~ 120 ℃ state and filtered in 200 mesh to obtain a filtered flaxseed oil, the natural flapseed filtered linseed oil 10 to 20 days to recover the upper layer Since only the flaxseed oil is obtained through the process, only the flaxseed oil component can be obtained, and the flaxseed component has a odor, such as a odor, and has a cyanide (-CN) toxicity. Can be. Therefore, the flaxseed oil obtained in the present invention can be prepared for the production of flaxseed oil is completely eliminated malodor and cyanide (-CN) toxicity.

Flaxseed oil of the present invention is to remove the odor, such as rotten egg smell or gurin smell, and to remove cyanide (-CN) toxicity during the purification process. Flaxseed oil of the present invention can be used as food and feed because it is removed odor and cyanide (-CN) toxicity and does not cause rancid even if exposed to air for a long time. In addition, the water-soluble linseed oil-containing food additive composition of the present invention is epigallocatechin-3-gallate (EGCG, C ₂₂ H ₁₈ O ₁₁ , molecular weight 458.4), epigallocatechin (EGC, C ₁₅ H ₁₄ O ₇ , molecular weight 306.3) and epicatechin-3-gallate (ECG, C ₂₂ H ₁₈ O ₁₀ , molecular weight 442.5) contains a mixture of green tea extract, which does not cause rancidity in the air. It smells sweet.

In order to produce a product containing a large amount of omega-3 fatty acid, the water-soluble flaxseed oil-containing feed additive composition from which odor and cyanide (-CN) toxicity are removed is taste-saving and well digested, so that the feed additive composition of the present invention is used. Even if the product is stored for a long time, it does not cause rancid in the air and can always have a delicious and crunchy taste.

If the product produced using the water-soluble linseed oil-containing food additive composition of the present invention, even if stored for a long time, it does not cause rancid in the air and can always have a crisp and crunchy taste. Can be kept as it is.
Green tea extract used in the present invention is preferably from 10 to 50% by weight, there is a problem that the flaxseed oil may be rancid if less than 10% by weight and the content of omega-3 fatty acid is lowered if more than 50% by weight. The emulsifier used in the present invention is contained in 5 to 20% by weight, if 5% by weight or less, there is a problem that the uniform mixing is not good, if more than 20% by weight, there is a problem that the content of omega-3 fatty acid is lowered, in the emulsifier Preference is given to using Tween 60.
The green tea extract used in the present invention was first extracted with green tea (Theae sinensis L., Theaceae-var.bohea nayabugida, Jejudosan) with an ethanol solution at 85 to 90 ° C. for 3 hours, and then, under the same conditions, 2 The tea extraction was completed, concentrated in a vacuum rotary evaporator, diluted 2 to 3 times by adding water to the concentrate, centrifuged to completely remove the solid component, and then the collected supernatant was extracted twice with 1.2 times chloroform to remove the chloroform layer. Then, the aqueous layer was extracted four times with 1.2 times ethyl acetate, and the obtained liquids were combined and dried under reduced pressure until the extraction solvent was completely removed. The dried product was dissolved in a small amount of water to obtain a green tea extract extract.

Example 1 Obtaining Green Tea Extracts Used in Water-Soluble Flaxseed Oil-Containing Feed Additives Eliminating Odor and Cyanide (-CN) To Produce Products Containing High Omega-3 Fatty Acids

1000 g of green tea (Theae sinensis L., Theaceae-var.bohea yabugida, Jeju Island) was extracted with 10 l of 70% (v / v) ethanol aqueous solution at 85 to 90 ° C for 3 hours, followed by the same conditions with 5 l of the same solvent. After completion of the second extraction, it was concentrated in a vacuum rotary evaporator to about 1000 mL. Water was added to the concentrate, diluted 2-3 times, centrifuged at 14,000 rpm for 20 minutes to completely remove solids, and the collected supernatants were extracted twice with 1.2-fold chloroform. Then, the chloroform layer was removed, the aqueous layer was extracted four times with 1.2 times ethyl acetate, and the obtained solution was combined, and dried under reduced pressure until the extraction solvent disappeared completely. The dried product was dissolved in a small amount of water to obtain a green tea extract extract.

Example 2 Obtaining Green Tea Extract Used in Water-Soluble Flaxseed Oil-Containing Feed Additive Compositions Eliminating Odor and Cyanide (-CN) To Produce Products Containing Large Omega-3 Fatty Acids

1000 g of green tea (Theae sinensis L., Theaceae-var.bohea nayabugida, Jeju Island) was extracted with 10 l of 70% (v / v) ethanol solution for 3 hours at 85 to 90 ° C, and then the same conditions were applied with 5 l of the same solvent. After completion of the second extraction, it was concentrated in a vacuum rotary evaporator to about 1000 mL. Water was added to the concentrate, diluted 2-3 times, centrifuged at 14,000 rpm for 20 minutes to completely remove solids, and the collected supernatants were extracted twice with 1.2-fold chloroform. Then, the chloroform layer was removed, the aqueous layer was extracted four times with 1.2 times ethyl acetate, and the obtained solution was combined, and dried under reduced pressure until the extraction solvent disappeared completely. The dried product was dissolved in a small amount of water and lyophilized to obtain a powdered green tea catechin extract.

In addition, as a result of measuring the content of the catechin contained in the powdered green tea catechin extract obtained by a known method (see Korean Patent Publication No. 2001-84165), with respect to the weight of the obtained green tea catechin powder, epi Gallocatechin-3-gallate 37.5% (w / w), epigallocatechin 12.5% (w / w), epicatechin-3-gallate 5.7% (w / w) and the balance of other catechins Could know.

Green tea is obtained by processing young leaves of the tea tree [Thae sinensis L. (Theaceae)] in early spring, and it has been reported that the green tea components exhibit various biological activities. As a representative example, anti-cancer effect, antioxidant effect, antimutagenic effect, skin protection effect, neuroprotective effect, anti-inflammatory effect, cell death were reported using green tea extract and components separated from green tea, and catechins component of green tea Has been reported to have anticancer and antioxidant effects. In addition, epigallocatechin-3-gallate (EGCG) and (-)-epigallocatechin (EGC), known as the main active ingredients of green tea, have anti-cancer effects, antioxidant effects, and skin protection. It has been reported that there is an effect, a cell death effect, polyphenol (green tea) also has anti-inflammatory effect, cancer prevention effect, antioxidant effect, brain cell protection effect.

Example 3 Odor and Cyanide (-CN) Toxicity for Use in Water-soluble Flaxseed Oil-Containing Feed Additives Removed Odor and Cyanide (-CN) Toxicity to Produce Products Containing High Omega-3 Fatty Acids Preparation of the Removed Water-Soluble Flaxseed Oil-Containing Feed Additive Composition

The flaxseed was heated at 100 ° C. for 20 minutes to remove moisture from flaxseed and then rapidly cooled. The fast-cooled flaxseed was placed in an milking machine and milked at a temperature of 100 ° C. in a milking machine, and then filtered at 200 mesh to obtain filtered flaxseed oil. After 20 days of natural sedimentation of the filtered flaxseed oil to obtain a purified flaxseed oil through a process of recovering the upper portion to prepare the flaxseed oil from which the odor and cyanide (-CN) toxicity was removed.

Analysis of the flaxseed oil contained 55.12% by weight of linolenic acid, eicosapen aenoic acid and docosahexaenoic acid, and odor and cyanide (-CN) toxicity. It was confirmed that this was removed.

(See Fig. 1 Test Analysis Result)

50 g of flaxseed oil, epigallocatechin-3-gallate (EGCG, C ₂₂ H ₁₈ O ₁₁ , molecular weight 458.4), epigallocatechin (EGC, C ₁₅ H) from which the malodor and cyanide (-CN) toxicity were removed ₁₄ g ₇ , molecular weight 306.3) and 20 g of green tea extract extract (ECG, C ₂₂ H ₁₈ O ₁₀ , molecular weight 442.5) containing 20 g of water (70% by weight of 30% water) mixed with 25 g of water Feed with 5 g of emulsifier (Twen 60: Tween 60) at high speed of 3000rpm and coated with high-speed rotation at 3000rpm to remove odor and cyanide (-CN) toxicity to produce foods containing large amounts of omega-3 fatty acids. An additive composition was prepared.

Water-soluble flaxseed without malodor and cyanide (-CN) toxicity as a result of analysis of water-soluble flaxseed oil-containing feed additive composition to remove malodor and cyanide (-CN) toxicity to produce products containing large amounts of omega-3 fatty acids The milk-containing feed additive composition contained 54.77% omega-3 fatty acids (see FIG. 2 Test Analysis Results).

Example 4 A method for producing omega-3 fatty acid eggs containing a large amount of omega-3 fatty acids

Clean water was diluted 1000-fold in a water-soluble linseed oil-containing feed additive composition from which malodor and cyanide (-CN) toxicity were removed to produce a product containing a large amount of omega-3 fatty acid prepared in Example 3, and the composition was added to a laying hen. When fed, it can be efficiently absorbed in the body of the laying hens without physiological sequelae, and the n-6 fatty acids in the eggs are drastically reduced and a large amount of n-3 fatty acids are accumulated (see FIGS. 3 to 7 test analysis results).

In order to perform a comparative experiment of fatty acid composition present in the eggs produced by the composition for laying hens of the present invention, the number of laying hens is divided into two groups, and the first group (control group) is supplied with commercially available feed for laying hens, and the second group ( Experimental group) was fed the laying composition for 30 days each of the present invention. The percentage of eggs produced in each group was measured and their averages were compared.

The composition ratio of n-6 / n-3 fatty acid in the eggs produced by the laying hens raised with the commercial feed (control) and the laying composition (experimental group) of the present invention was investigated. Although the n-6 / n-3 fatty acid ratio was 11.8 in eggs produced from commercially fed laying hens (control group), the n-6 fatty acids were significantly higher, but they were produced in laying hens (experimental group) fed the laying hen composition of the present invention. The ratio of n-6 / n-3 fatty acids in one egg began to increase on the third day after feeding, and rapidly increased on the sixth day. As of 1.3, n-3 fatty acids increased greatly and showed the same value over time. Only 10 days after feeding the composition for laying hens to the laying hens, a very high ratio of n-3 fatty acids was obtained. Accumulated eggs of a large amount of n-3 fatty acids, such as the ratio of n-6 / n-3 fatty acids to 1.3, have not been successful in any studies yet. It is an egg.

Eggs produced by feeding the composition for laying hens to the laying hens have a low content of n-6 fatty acids and a large amount of n-3 fatty acids are accumulated, and various physiological diseases or sequelae caused by n-6 fatty acids occur when ingested. It is possible to prevent various physiological diseases due to excessive n-6 fatty acid intake, and to maintain the physiological balance in the body by reducing the ratio of n-6 / n-3 fatty acids. Thus, until now, there was a tendency to avoid ingestion of eggs, especially egg yolk, due to the recognition that excessive saturated fatty acids or n-6 fatty acids are present in the eggs, but the eggs produced by feeding the laying composition of the present invention to the laying hens are n While the -6 fatty acid content has been reduced, the n-3 fatty acid content has been greatly increased, allowing for the consumption of much larger eggs than ever before without concern of any side effects.

Example 5 A method for producing omega-3 fatty acid milk containing a large amount of omega-3 fatty acid

The composition was milked by diluting clean water 1000 times with water-soluble linseed oil-containing feed additive composition from which malodor and cyanide (-CN) toxicity were removed to produce a product containing a large amount of omega-3 fatty acid prepared in Example 3. When fed to the cow's body can be efficiently absorbed without physiological sequelae in the cow's body, and the n-6 fatty acids in the milk is drastically reduced and a large amount of n-3 fatty acids accumulated (see Fig. 8 to 10 test analysis results).

To compare the fatty acid composition present in the milk produced by the composition for milk production of the present invention, 40 cows were divided into two groups and the first group (control group) was supplied with commercially available milk production feed, and the second The group (experimental group) was fed the milk production composition of this invention for 30 days, respectively. The proportion of milk produced in each group was measured and the average value was compared.

The composition ratio of n-6 / n-3 fatty acids in milk produced by commercial feed (control) and cows raised with the milk production composition (experimental group) of the present invention was investigated. In the milk produced from commercial feed-fed cows (control), the n-6 / n-3 fatty acid ratio was 11.8, which was significantly higher in n-6 fatty acids, but in cows fed the milk-producing composition of the present invention (experimental group). The ratio of n-6 / n-3 fatty acids in milk produced increased sharply from day 3 after n-3 fatty acids began to increase, and from day 10 the ratio of n-6 / n-3 fatty acids began to increase. As this 1.3, n-3 fatty acids increased greatly and showed the same value continuously over time. Only 10 days after feeding the cow composition of the present invention to the cow was able to obtain a very high milk ratio of n-3 fatty acids. Accumulated milk of a large amount of n-3 fatty acids, the ratio of n-6 / n-3 fatty acids to 1.3, has not been successful in any studies yet and the highest level of n-3 fatty acids produced so far. It contains milk.

Milk produced by feeding the composition for milk production of the present invention to cows has a low content of n-6 fatty acids and a large amount of n-3 fatty acids are accumulated. It does not occur, it is possible to prevent various physiological diseases due to excessive n-6 fatty acid intake and to maintain the physiological balance in the body by reducing the ratio of n-6 / n-3 fatty acids. Therefore, until now, there was a tendency to avoid milk because of the recognition that excessive saturated fatty acids or n-6 fatty acids are present in the milk, but the milk produced by feeding the milk composition for milk production of the present invention to cows has reduced n-6 fatty acid content. On the other hand, the amount of n-3 fatty acids has been greatly increased, allowing much more milk to be consumed than ever before.

In addition, in order to measure the degree of transition of unsaturated fatty acid into milk in the milk fed the feed additive of the present invention was performed in vivo using a cow. That is, the feed additives were mixed in the cow's feed and fed with milk 250g per day for 28 days, milk was collected at 1, 2, and 4 weeks to analyze fatty acids. The results of the analysis showed that the milk containing the feed additives contained a large amount of EPA and DHA, and the ratio of saturated fatty acids was significantly reduced in the ratio of saturated fatty acids and unsaturated fatty acids in the milk.

The feed additive prepared according to the method of the present invention transfers polyunsaturated fatty acids such as DHA and EPA to milk with high efficiency to prevent various adult diseases such as hypertension and heart disease, and the feed additive is not decomposed by microorganisms in the cow's first place. It is bypassed to allow highly polyunsaturated fatty acids to be transferred to the final product milk with high efficiency.

The feed additive of the present invention has a very excellent effect in producing milk with a high content of polyunsaturated fatty acids and a significantly reduced content of saturated fatty acids, and the present invention provides a method of preparing such feed additives to improve the health of national health. It will contribute to the improvement and prevention of adult diseases.

Example 6 A method for producing omega-3 fatty acid chicken meat containing a large amount of omega-3 fatty acid

Clean water was diluted 1000-fold in a water-soluble flaxseed oil-containing feed additive composition from which malodor and cyanide (-CN) toxicity were removed to produce a product containing a large amount of omega-3 fatty acid prepared in Example 3, and the composition was treated to chicken. When fed, the chicken body can be efficiently absorbed without physiological sequelae in the chicken body, and the n-6 fatty acid in the chicken is drastically reduced and a large amount of n-3 fatty acid is accumulated (see FIG. 11 test analysis result).

Example 7 A method for producing omega-3 fatty acid beef containing a large amount of omega-3 fatty acid

Clean water was diluted 1000-fold in a water-soluble flaxseed oil-containing feed additive composition from which malodor and cyanide (-CN) toxicity were removed to produce a product containing a large amount of omega-3 fatty acid prepared in Example 3, and the composition was treated to cows. Feeding could be efficiently absorbed without any physiological sequelae in the cow's body, and it rapidly reduced n-6 fatty acids in beef and accumulated large amounts of n-3 fatty acids.

Feeding the flaxseed oil feed additive prepared according to the above example in livestock, it is possible to produce (1) meat having a high content of unsaturated fatty acids, especially high omega fatty acids and low cholesterol, instead of meat having a high content of saturated fatty acids and cholesterol, and (2) It has a taste that is suitable for livestock, and (3) can be added to blended feed or directly to feed on site, easy to store and transport, and (4) fat without the use of antioxidants (BHT, BHC). It can be stored for a long time at room temperature, and can prevent oxidation of these substances and increase absorption in the small intestine, thereby producing excellent beef that does not harden marbling.

Therefore, the linseed oil feed additive is added to the total feed based on the fat content of 5% and fed to the pigs, and then the omega-3 fatty acids accumulated in the microsomes of the liver of the liver, the loin of the meat and the subcutaneous fat. The omega-3 fatty acids accumulated in the microsomes of pig liver were 11.3% linolenic acid, 10.72% Eicosapen aenoic acid and docosahexaenoic acid. 7.7% of docosahexaenoic acid, and the omega-3 fatty acids accumulated in the lion are 2.47% linolenic acid, 2.25% Eicosapen aenoic acid and docosahexaeno It contains 11.34% of Docosahexaenoic acid, and the omega-3 fatty acids accumulated in subcutaneous fat were 1.0% linolenic acid, 0.73% Eicosapen aenoic acid and docosahexaenoic acid. (Docosahexaenoic acid) 0.7% It was contained. In addition, chicken fed flaxseed oil composition of the present invention diluted 1000-fold in clean water for 4 weeks contained 1,064.26 mg / 100g of omega-3. Eggs fed for 4 weeks by diluting the flaxseed oil composition of the present invention 1000 times in clean water contained 596.87 mg / 100g of omega-3. The flaxseed oil composition of the present invention diluted 1000-fold in clean water and fed for 4 weeks contained 190.59 mg / 100 g of omega-3. The milk fed four weeks by diluting the flaxseed oil composition of the present invention 1000 times in clean water contained 0.27% by weight of omega-3.

1 is a toxicity test report for flaxseed oil used in the present invention.

Figure 2 is a food test report for a water-soluble flaxseed oil-containing feed additive composition for producing a product containing a large amount of omega-3 fatty acids of the present invention.

3 to 7 is a food test report for eggs fed a water-soluble linseed oil-containing feed additive composition for producing a product containing a large amount of omega-3 fatty acids of the present invention.

8 to 10 is a food test report for milk fed a water-soluble linseed oil-containing feed additive composition for producing a product containing a large amount of omega-3 fatty acids of the present invention.

11 is a food test report for chickens fed a water-soluble flaxseed oil-containing feed additive composition for producing a product containing a large amount of omega-3 fatty acids of the present invention.

Claims (5)

In the water-soluble linseed oil-containing feed additive composition in which the odor and cyanide (-CN) toxicity added to chicken feed, pig feed, and bovine feed are removed, the feed additive composition is used to keep flaxseed at 80 to 120 ° C. for 10 to 40 minutes. Heated to remove moisture from flaxseed and then rapidly cooled, put the fast-cooled flaxseed in the milking machine milked at the milking machine temperature of 80 ~ 120 ℃ and filtered in 200 mesh to obtain a filtered linseed oil, the filtered flaxseed oil 50 to 90% by weight of flaxseed oil from which malodor and cyanide (-CN) toxicity were removed by obtaining purified flaxseed oil through a process of spontaneous sedimentation for 10 to 20 days to recover the upper layer; And The green tea was first extracted with an ethanol aqueous solution at 85 to 90 ° C. for 3 hours, and then the secondary extraction was completed at 85 to 90 ° C. for 3 hours with an ethanol aqueous solution, and then concentrated in a vacuum rotary evaporator, and water was added to the concentrate. After dilution with pear and centrifugation to completely remove the solid component, the collected supernatant was extracted twice with 1.2 times of chloroform to remove the chloroform layer, and the aqueous layer was extracted four times with 1.2 times of ethyl acetate. After combining, the extract was dried under reduced pressure until the solvent completely disappeared, and the dried product was dissolved in water and lyophilized to produce epigallocatechin-3-gallate (EGCG, C ₂₂ H ₁₈ O ₁₁ , molecular weight 458.4), epi 10-50% by weight of green tea extract containing gallocatechin (EGC, C ₁₅ H ₁₄ O ₇ , molecular weight 306.3) and epicatechin-3-gallate (ECG, C ₂₂ H ₁₈ O ₁₀ , molecular weight 442.5) Said flax Odor and cyanide to produce a product containing omega-3 fatty acid, characterized in that the oil and edible emulsifiers added to the green tea extract prepared by mixing 5 to 20% by weight compared to the mixture of the flaxseed oil and green tea extract (-CN) A water-soluble flaxseed oil-containing feed additive composition with no toxicity. In order to produce a product containing the omega-3 fatty acid prepared in claim 1, the odor and cyanide (-CN) toxicity of the water-soluble linseed oil-containing feed additive composition from 0.5 to the total amount of the feed to the cows Mixing 10% by weight to feed the cows; And 0.05 to 10% by weight of a water-soluble flaxseed oil-containing feed additive composition in which malodor and cyanide (-CN) toxicity have been removed in order to produce a product containing omega-3 fatty acids, relative to the total amount of clean water fed to cows. Omega-3, characterized in that it is fed to the cow in any one of the method of feeding to the cow to produce omega-3 fatty acid milk containing 2 to 10 times the omega-3 fatty acid compared to the cow's milk Process for producing milk containing fatty acids. In order to produce a product containing the omega-3 fatty acid prepared in claim 1 to 0.5 to the total amount of the feed to the chicken feed water-soluble flaxseed oil-containing feed additive composition is eliminated malodor and cyanide (-CN) toxicity Mixing 10% by weight to feed chickens; And 0.05 to 10% by weight of a water-soluble flaxseed oil-containing feed additive composition in which malodor and cyanide (-CN) toxicity have been removed to produce a product containing omega-3 fatty acids, relative to the total amount of clean water fed to chickens. Omega-3 fatty acid, characterized in that the chicken is fed by any one of the methods of feeding the chicken to produce the omega-3 fatty acid chicken containing 2 to 10 times the omega-3 fatty acid compared to the normal chicken Method of producing chicken meat containing. In order to produce beef containing the omega-3 fatty acid prepared in claim 1, the odor and cyanide (-CN) toxicity of the water-soluble linseed oil-containing feed additive composition is 0.5 to about the total amount of the feed to the cows 10% by weight of cows to mix; And 0.05 to 10% by weight of a water-soluble flaxseed oil-containing feed additive composition in which odor and cyanide (-CN) toxicity are removed to produce a product containing omega-3 fatty acids, relative to the total amount of clean water fed to cattle. The omega-3 fatty acid, characterized in that to produce the omega-3 fatty acid beef containing 2 to 10 times the amount of omega-3 fatty acid compared to normal beef by feeding to the cow by any one method selected from the method of feeding to the cow Method of producing beef containing. delete

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