KR20100082621A - Development of cage for the cultivation of fruit flies - Google Patents

Abstract

PURPOSE: A culture container for culturing Drosophila adult is provided to observe Drosophila lifetime, egg collection, and fecundity. CONSTITUTION: A culture container for culturing Drosophila adult comprises: a culture container(100) which is formed in a transparent body; and a cover(140) at the upper portion of the culture container, having a net. A magnet is mounted at the upper outside of the culture container. A culture fixing plate(130) is mounted for preventing gap of culture insertion part of the culture container. A hole is formed at the center to insert culture container.

Description

Development vessel for adult cultivation of fruit flies {Development of cage for the cultivation of fruit flies}

The present invention relates to a culture vessel for adult Drosophila cultivation, and more specifically, it is possible to observe the adult culture and change of Drosophila, and to provide a Drosophila cultivator dedicated incubator and also to provide a Drosophila life observation incubator It greatly improved the quality and reliability of the researcher so that the researchers could plant a good image.

As is well known, fruit flies are very useful for studying the effects of life on a variety of stimuli compared to other animal models because they are smaller in size, easier to grow in the laboratory, and less expensive to maintain. In particular, more than 70% of human disease-related gene analogues exist in the genome of fruit flies, and are attracting attention as various disease model animals. In addition, fruit flies have various life cycles of eggs, larvae, pupa, and adults, and have a relatively short lifespan of about 60 days. In this respect, Drosophila is a very suitable animal as a research model for understanding the effects of external stimuli on human lifespan. However, despite the fact that it is an animal material that improves the efficiency of research, many researchers have not been able to easily use it because of difficulty in cultivating fruit flies. In other words, fruit flies regularly change their medium to provide fresh and rich nutrition for their growth. However, if you want to cultivate dozens of fruit flies in a limited medium in a single container for a long time, there is a hassle of moving the fruit flies for medium supply once a certain period. Moreover, since the amount of medium is also placed in a limited container, the number of fruit flies that can be cultured in the container is limited, and in order to obtain statistically significant results, it is difficult to use a large number of containers. And despite all these limitations, the enormous amount of time and labor it took was one of the biggest reasons for not using fruit flies easily.

In order to solve the above problems, Japanese Patent Laid-Open No. 2002-017204 has been filed.

That is, the above-described prior art has a transparent container body and a cover freely attached or detachably mounted on the transparent container body, and the lid has a top plate and an outer circumferential wall extending downward from the outer periphery of the top plate. And an insect-like ornamental breeding container provided with a plurality of vent holes in part or all of the outer circumferential wall.

In addition, in the prior art, a separate adult food supply and a water supply and communicating with the outside through a food supply pipe and a water supply pipe; It is equipped with adult fables and eggplants, which are detachably connected to the outside through an allegorizer tube and a eggplant tube, and all surfaces except the lower surface are made of a mesh of 1 ~ 2mm mesh. Provided is an adult breeding device, characterized in that a chaeran medium made of a jelly type is prepared by mixing pig meal, fish meal and gelatin.

However, the above-described prior art has a problem that it is very difficult to use because it is not a mass breeding device.

In addition, the above-described conventional technology has a problem that can not easily remove the dead fruit fly in the structure, and minimize the external exposure of the internal space.

The present invention has been made in order to solve the problems of the prior art as described above, and design a new culture vessel (cage) to use a conventional culture bottle (vial; diameter 25mm x height 95mm) as a medium vessel In one case, the conventional vial culture vessel was able to accommodate only up to 30 fruit flies due to its small size, so a large number of vial had to be used to secure the population for statistical processing. In order to overcome this problem, it was designed to accommodate 40 times more culture space and accommodate up to 1000 or more, and to improve space utilization, the first purpose was to develop a cuboid rather than a cylinder. Drosophila is widely used as a model animal for checking the lifespan, but the conventional method has to change the vial culture vessel daily to remove the fruit flies attached to the medium. This method wasted unnecessary costs by changing the medium without considering nutritional status to verify its lifespan, and had to bear the loss due to escape during the movement of the fruit fly. However, in the present invention, in order to overcome these disadvantages, a drawer is installed at the bottom of the culture container to easily remove the dead fruit flies. Furthermore, in order to prevent the loss of fruit flies due to escape, the space created during the opening and closing of the drawer was closed with a drawer to prevent the escape of living fruit flies. This fruit flies were developed using drinking oil, that is, the habit of moving against gravity, and the fourth purpose was to make the fruit flies easily by making a lid on the upper layer, and to facilitate air circulation through the network structure. The fifth objective is to develop such a new type of culture vessel, as well as to observe the life of the adult under various environmental conditions (temperature, humidity, light, gravity, various stress conditions). Therefore, it is intended to be used in various ways such as egg collection and fecundity measurement, and the sixth object is that the fruit fly adult to greatly improve the quality and reliability of the product, so that researchers can plant good images. Provide a culture vessel for culturing.

In order to achieve the above object, the present invention includes at least one medium container inserted into one side of the body, and the culture container formed of a transparent body so that the fruit fly adult culture, fruit fly receiving, fruit fly life observation and the inside can be checked from the outside. It provides a culture vessel for adult Drosophila culture.

As described in detail above, the present invention design and manufacture a new culture vessel (cage) in order to use a vial (vial; diameter 25mm x height 95mm), which is an existing culture vessel, as a medium vessel. Because of its size, up to 30 fruit flies could be accommodated, so a number of vial bottles had to be used to obtain populations for statistical processing. To overcome this, it was designed to accommodate 40 times more culture space and accommodate up to 1000 or more, and was developed in the form of a cuboid rather than a cylinder to increase space utilization.

And the fruit flies of the present invention is used as a high value as a model animal for confirming the life, but the conventional method had to change the vial culture vessel daily to remove the fruit flies attached to the medium. This method wasted unnecessary costs by changing the medium without considering nutritional status to verify its lifespan, and had to bear the loss due to escape during the movement of the fruit fly. However, in the present invention, the drawer is installed at the bottom of the culture container to overcome these disadvantages, and thus, the fruit flies can be easily removed by removing the dead, and the unnecessary medium is removed to prevent the cost loss.

In addition, the present invention prevents the escape of live fruit flies by blocking the space generated during opening and closing of the drawer with a drawer to prevent the loss of fruit flies due to escape. This fruit flies were developed using drinking oil, that is, the habit of moving against gravity, and the fourth purpose was to make the fruit flies easily by making a lid on the upper layer, and to facilitate air circulation through the network structure. It is.

In addition, the present invention is not only to observe the life of the adult under various environmental conditions (temperature, humidity, light, gravity, various stress conditions) until now, the development of this new type of culture vessel, depending on the application of the culture vessel (egg collection), fertility (fecundity) measurement, etc. can be used in various ways.

The present invention is a very useful invention that can significantly improve the quality and reliability of the product due to the above-described effect so that researchers can plant a good image.

Hereinafter, described in detail with reference to the accompanying drawings a preferred embodiment of the present invention for achieving this effect are as follows.

Culture vessel for adult Drosophila cultivation applied to the present invention is configured as shown in Figures 1 to 42.

In the following description of the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

The following terms are terms set in consideration of functions in the present invention, and may be changed according to the intention or custom of the producer, and the definitions thereof should be made based on the contents throughout the present specification.

First, at least one medium container 105 is partially inserted and installed at one side of the body 101, 201, 301, Drosophila adult culture, Drosophila receiving, Drosophila life observation and the inside from the outside To provide a culture vessel for culturing adult Drosophila is provided with a culture vessel (100, 200, 300) formed of a transparent body to confirm.

At this time, the culture vessel 100 as a first embodiment of the present invention to observe the development and growth process of fruit flies, to observe the changes of fruit flies due to various stresses, can easily replace the medium without the loss of fruit flies, drawer It is configured to remove dead fruit flies.

In addition, the culture vessel 200 is capable of cultivating a large number of fruit flies as a second embodiment of the present invention, a dedicated drawer for receiving eggs is installed, and a large amount of eggs can be collected from a large number of fruit flies. It is configured to easily replace the medium during the birth.

In addition, the culture vessel 300 is a third embodiment of the present invention by lowering the position of the medium is possible to supply the medium to low activity Drosophila, and to remove the dead fruit flies to remove the drawer instead of inserting, inserting the tube Dead fruit flies can be easily removed and accurate counting of the removed fruit flies for statistical processing.

The technical configuration described above will be described in more detail as follows.

That is, as shown in Figure 10, 11, 12, 26, 27, 28, 38, 39, 40, the lid 140, 240 (on the top of the culture vessel 100, 200, 300) ( 340 is installed, but the lids 140, 240 and 340 are provided with nets 141, 241 and 341 for air circulation.

At this time, the meshes 141, 241 and 341 are preferably made of stainless steel to prevent corrosion.

In addition, as shown in Figures 2, 18, 33, the lids 140, 240, 340 applied to the present invention closely to the top of the culture vessel 100, 200, 300 without shaking by magnetic force Magnets 142, 242, 342 are installed on the inner side of the lid 140, 240, 340 to be attached, and the magnet 143 on the top outer side of the culture vessel 100, 200, 300 243 and 343 are installed.

15, 16, 31, 32, 41, 42, one side of the culture vessel 100, 200, 300 is inserted into the culture vessel 105 applied to the present invention of the culture vessel Badge fixing plates 130, 230 and 330 are installed to prevent a gap of the medium inserting portion, and holes 131 and 231 are fitted in the center of the badge fixing plates 130, 230 and 330, respectively. 331 is formed.

In addition, the present invention, the medium fixing plate 130, 230, 330 is fixed to the side of the culture vessel (100, 200, 300) without shaking by the magnetic force medium fixing plate 130, 230, 330 One side of the magnets 132, 232, 332 is installed, the magnets 133, 233, 333 is also installed on the outer surface of the culture vessel.

At this time, the present invention, as shown in Figures 41 and 42, the opening of the hole 331 is normally blocked in the hole 331, when the dead fruit flies are removed by the suction machine (footer) tube (in the drawing) A rubber plate 333 to be inserted is installed.

At this time, the rubber plate 333 can be used to form a cross-shaped notch groove so that the tube is easily inserted.

In addition, the drawer 110, 210 and the drawer on the bottom of the body 101, 201 applied to the present invention to easily remove the dead fruit flies, as shown in Figures 1 to 6, 17 to 22 The drawer pedestals 102 and 202 are provided so that the drawers 110 and 210 can be fixed when the 110 and 210 are opened.

At this time, when the drawer 110 is inserted into the body 101, the bottom bottom of the body 101, 201 so that the drawers 110, 210 are closely attached to the body 101, 201 without shaking by magnetic force. Magnets 103 and 111 are respectively installed in the and drawers 110 and 210.

In addition, as shown in FIGS. 23, 24, and 25, both ends of the drawer 210 are provided with plate-shaped grooves 212 for receiving eggs to observe the entire fruit fly life history such as eggs, larvae, pupa, and adults. .

In addition, the present invention as shown in Figure 13, 14, 29, 30 drawer to minimize the internal exposure of the culture vessel when the drawer 110, 210 in front of the body 101, 201 is opened The membranes 120 and 220 are further installed, and the drawer membranes 120 and 220 are configured to move up and down on the rails 104 and 204 formed on both sides of the bodies 101 and 201. .

Finally, the present invention has a magnet 121 on one side of the drawer membrane 120, 220 so as to be closely attached to the body 101, 201 without shaking by magnetic force when the drawer membrane 120, 220 is closed. 221 is installed, and the magnets 122 and 222 are also installed on the outer circumferential surfaces of the bodies 101 and 201, respectively.

On the other hand, the present invention may be variously modified and may take various forms in applying the above configuration.

And it is to be understood that the invention is not limited to the specific forms referred to in the above description, but rather includes all modifications, equivalents and substitutions within the spirit and scope of the invention as defined by the appended claims. It should be understood that.

Referring to the effect of the culture vessel for the adult fruit fly adult culture configured as described above are as follows.

First, in the present invention, a new culture vessel (cage) 100, 200, 300 was designed and manufactured in order to use a conventional culture vessel (vial; diameter 25mm x height 95mm) as a medium vessel. Conventional vial culture vessels could only accommodate up to 30 fruit flies due to their small size, so many vial bottles had to be used to secure populations for statistical processing. In order to overcome this problem, the present invention was designed to accommodate up to 1000 or more cultures by securing a culture space of 40 times or more, and developed in a cuboid type rather than a cylinder to increase space utilization. Drosophila is also widely used as a model animal for the purpose of confirming its lifespan, but the conventional method has to change the vial culture vessel daily to remove the fruit flies attached to the dead. This method wasted unnecessary costs by changing the medium without considering nutritional status to verify its lifespan, and had to bear the loss due to escape during the movement of the fruit fly. However, in the present invention, drawers 110 and 210 are installed at the bottom of the culture containers (cage) 100, 200, and 300 to overcome these shortcomings. By eliminating the movement, the cost loss is prevented. Furthermore, in order to prevent the loss of fruit flies due to escape, the spaces generated when the drawers 110 and 210 are opened and closed are prevented by the drawer curtains 120 and 220 to prevent escape of live fruit flies. This fruit flies were developed using drinking intelligence, a habit of moving against gravity. And the lid 140, 240, 340 in the upper layer to make it easy to move the fruit flies, and to facilitate the air circulation through the network structure.

The development of this new type of culture vessel has not only observed the life of an adult under various environmental conditions (temperature, humidity, light, gravity, and various stress conditions), but also according to the application of the culture vessel (egg collection). ), Fecundity measurement, etc. can be used in various ways.

When explaining the more specific effect of the present invention.

In other words, the present invention focused on reducing the time required for the culture of the fruit fly and the number of vial culture vessels.

First, the culture vessels 100, 200, and 300 were made of transparent acrylic plates so that the growth state of the fruit flies to be cultured can be easily observed in the milk duct. It also takes into account the circadian rhythm of fruit flies, allowing them to receive light and dark stimuli without disturbances.

In addition, the chemistry using odor by installing a network (141, 241, 341) of the network structure (141, 241, 341) for the air circulation of the sealed culture vessel (100, 200, 300) It can be applied to the chemotaxis. The basis for life observation is to separate the living from the dead. By calculating dead flies, you should be able to easily remove them so that they do not overlap when calculating survival rates. Since the fruit flies fall to the floor when they die, it was early to install the drawers 110, 210 in the culture vessel 100, 200.

Above all, the biggest problem in the present invention was that outflow of fruit flies to the outside may occur due to the gap caused by the expansion of the culture vessel. Drosophila is usually only 2mm in size, so even small spaces can leak out through it. In the past, some research teams have attempted to develop such culture vessels, but the biggest reason for their failure was that they did not close this gap. In particular, the instantaneous external exposure that may occur during the movement of fruit flies and the change of medium was also a problem to be solved. In order to solve this problem, in the present invention, first, the seam parts (for example, drawers, discharge slots, lids, etc.) are overlapped to avoid direct exposure to the outside, and magnets are installed at each part to fix the parts without shaking. The device was developed. In order to block spatial contact with the outside of the drawers 110 and 210, which may occur even when the drawers 110 and 210 are opened and closed, the drawer walls 120 and 220 are formed on the walls of the culture vessels 100 and 200. When installed in the drawer (110, 210) was opened to minimize the exposure of the interior of the culture vessel (100, 200). In particular, the cylindrical medium container is designed to fit with the fixed plate installed with a magnet does not generate a gap of 0.1mm, using the fixed plate to be installed in close contact with the new culture vessel in a vertical state. This has also created the advantage of minimizing external exposure that may occur when changing media.

In addition, the culture vessels 100, 200, 300 applied to the present invention, the nets 141, 241 and 341 of the lids 140, 240 and 340 are made of stainless steel to prevent corrosion and chemical reactions. Application to the experiment was made.

In addition, the application of the Drosophila culture containers 100, 200, 300 applied to the present invention can be made in various ways. Basically, the adult fly fruit flies in the culture vessels 100, 200 and 300 can be easily replaced without moving the fly fruit, thereby preventing the loss of fruit flies in the experimental process. All components of the culture vessel (100, 200, 300) is fixed to the magnet has a feature that does not fall easily, the culture vessel (100) (200) (300) may be used by lying on its side. For example, the culture vessel can be laid on its side for the chemical periodicity test of fruit flies, and the desired substance can be located next to the networked lid to identify the fruit fly's tendency.

In addition, the present invention using a drawer 210 made of a plate size groove 212 for receiving eggs in addition to the basic type of drawer to observe at a glance the process of changing from eggs to larvae, chrysalis and adults in addition to simple life observation of the adult You may. In addition to such direct use, it may be applied in parallel with an infrared device for measuring mobility.

The technical idea of the culture vessel for adult fruit fly culture of the present invention is that the same result can be repeatedly carried out, and in particular, by carrying out the present invention, it is possible to promote technology development and contribute to industrial development, which is worth protecting.

1 is a real yarn of the culture vessel for adult fruit flies culture of the first embodiment of the present invention

        camp.

2 is a front view of FIG. 1;

3 is a rear view of FIG. 1.

4 is a right side view of FIG. 1;

5 is a left side view of FIG. 1;

6 is a plan view of FIG.

7 is a front view of the drawer applied in FIG.

8 is a side view of FIG. 7;

9 is a plan view of FIG.

10 is a plan view of the lid applied in FIG.

11 is a side view of FIG. 10.

12 is a front view of FIG. 10;

13 is a plan view of the drawer barrier applied to FIG.

14 is a front view of FIG. 13;

15 is a front view of the medium fixing plate applied in FIG.

16 is a side view of FIG. 15;

17 is a real yarn of the culture vessel for adult fruit fly culture of the second embodiment of the present invention

        camp.

18 is a front view of FIG. 17;

19 is a rear view of FIG. 17.

20 is a right side view of FIG. 17;

FIG. 21 is a left side view of FIG. 17; FIG.

22 is a top view of FIG. 17.

FIG. 23 is a front view of the drawer applied to FIG. 17; FIG.

24 is a side view of FIG. 23.

25 is a top view of FIG. 23.

FIG. 26 is a plan view of the lid applied to FIG. 17. FIG.

FIG. 27 is a side view of FIG. 26; FIG.

FIG. 28 is a front view of FIG. 26; FIG.

29 is a plan view of the drawer barrier applied to FIG. 17;

30 is a front view of FIG. 29;

FIG. 31 is a front view of the badge fixing plate applied to FIG. 17. FIG.

32 is a side view of FIG. 31;

33 is a front view of a culture vessel for culturing adult fruit flies according to the third embodiment of the present invention.

         Degree.

FIG. 34 is a rear view of FIG. 33;

35 is a right side view of FIG. 33;

36 is a left side view of FIG. 33;

FIG. 37 is a plan view of FIG. 33;

FIG. 38 is a plan view of the lid applied to FIG. 33. FIG.

39 is a side view of FIG. 38;

40 is a front view of FIG. 38;

FIG. 41 is a front view of a remover for applying to FIG. 33; FIG.

FIG. 42 is a side view of a remover for application to FIG. 33. FIG.

<Explanation of symbols for the main parts of the drawings>

100, 200, 300: culture vessel

101, 201, 301: body

110, 210 drawers

120, 220: drawer curtain

130, 230, 330: badge holding plate

140, 240, 340: lid

Claims (11)

At least one medium container 105 is partially inserted into one side of the body 101, 201, and 301, and is transparent to allow the inside of the fruit fly culture, the fruit fly receiving, the fruit fly life observation, and the inside to be identified from the outside. A culture vessel for adult culture of Drosophila, characterized in that the culture vessel formed by 100 (200) (300) is provided. The method according to claim 1, Caps 140, 240 and 340 are installed at the upper portion of the culture vessels 100, 200 and 300, and the caps 141, 241 and 341 are installed for air circulation. Culture vessel for adult fruit flies culture. The method according to claim 2, Magnets (142, 242, 342) on the inner surface of the lid so that the lid 140, 240, 340 is attached to the upper portion of the culture vessel 100, 200, 300 without close shaking by magnetic force And a magnet (143) (243) (343) is also installed on the top outer surface of the culture vessel. The method according to claim 1, One side of the culture vessel 100, 200, 300 is inserted into the culture vessel 105, the medium fixing plate 130, 230, 330 is installed to prevent the gap of the culture insert portion of the culture vessel, A culture vessel for culturing Drosophila adult, characterized in that holes (131) (231) (331) are formed in the center of the medium fixing plate so as to sandwich the culture vessel. The method according to claim 4, Magnets (132, 232, 332) on one side of the medium fixing plate so that the medium fixing plate 130, 230, 330 is closely attached to the side of the culture vessel (100, 200, 300) without shaking by magnetic force. Is installed, the culture vessel for adult fruit fly culture, characterized in that the magnet 133 (233) (333) is also installed on the outer surface of the culture vessel. The method according to claim 4, The hole 331 is a culture for fruit adult culture, characterized in that the rubber plate 333 to insert the tube of the footer (footer) is inserted when removing the dead fruit flies, which normally block the entrance of the hole by the suction machine Vessel. The method according to claim 1, The bottom of the body 101, 201 is further provided with a drawer 110, 210 to easily remove dead fruit flies, and drawer rests 102 and 202 so that the drawer is fixed when the drawer is opened. Culture vessel for culturing adult fruit fly, characterized in that. The method according to claim 7, Drosophila adult, characterized in that magnets 103 and 111 are installed on the bottom and the bottom of the body so that the drawer 110 is tightly attached to the body without shaking by magnetic force when the drawer 110 is inserted into the body 101. Culture vessel for cultivation. The method according to claim 7, Both ends of the drawer 210, the culture vessel for cultivating adult fruit fly, characterized in that the grooves 212 for receiving the egg size is formed so that the egg, larvae, chrysalis, adult, etc. can observe the overall life history of fruit flies. The method according to claim 1, The front of the body 101, 201 is further provided with a drawer membrane 120, 220 to minimize the internal exposure of the culture vessel when the drawer is opened, the drawer is a rail 104 formed on both sides of the body Culture vessel for adult fruit cultivation, characterized in that to operate up and down by riding on (204). The method according to claim 10, Magnets 121 and 221 are installed on one side of the drawer wall so as to be attached to the body 101 and 201 without shaking by magnetic force when the drawer wall 120 and 220 are closed, and an outer circumferential surface of the body Each of the magnets 122, 222 is a culture vessel for adult fruit cultivation, characterized in that the installation.

Images