KR20100061338A - Controlling method for diesease damage on brassicaceous plants - Google Patents

Abstract

PURPOSE: An extermination method of brassicaceous plants blight is provided to strongly control root nodule disease with the high outbreak rate, and to secure the improved preventing effect to a single strain. CONSTITUTION: An extermination method of brassicaceous plants blight comprises the following steps: contacting an agent containing variovorax sp. CGF4526 as an active ingredient with active ingredient; and contacting another agent including 2',4-dichloro-alpha,alpha,alpha-trifluoro-4'-nitro-m-toluenesulfone anilide as the active ingredient with soil to plant the brassicaceous plants. The brassicaceous plants blight is root nodule disease. The brassicaceous plants are chinese cabbage, sauerkraut, radish, cauliflower, broccoli, pak-choi or litigation herbs.

Description

Controlling method for diesease damage on brassicaceous plants}

The present invention provides a bacteriophage bacterium CGF4526 strain (Variovorax sp. CGF4526) as an active ingredient, and 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro- It relates to a method for controlling rapeseed and plant diseases, comprising contacting rapeseed and plants with an agent comprising m-toluenesulfonanilide as an active ingredient.

Plasmodiophorabrassicae Woronin, the major soil disease of rapeseed plant diseases, is a soil fungal disease that occurs in most plants of rapeseed plants, such as cabbage, cabbage, cauliflower, and broccoli. Root gall disease is hampering the stable production of these plains and crops. As a symptom, nodules are formed in the roots from about 20 days after planting, and in the early stages of infection, no cabbage or cabbage is formed in the cabbage or cabbage. I can't even see it.

Conventionally, a control agent using microorganisms as an active ingredient has been developed, and a report example by Bacillus bacteria (Patent Literature 1) and Aziboractus bacteria (Patent Literature 2) as a biological control method for rapeseed and plant root gall diseases so far. There is). As a report example related to this invention, there exists a report example (patent document 3) by the genus Barrioborax.

On the other hand, sulfonamide derivatives such as 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide have been known as herbicides and agricultural fungicides since ancient times. The prior art as a herbicide of a sulfonamide derivative is disclosed by patent document 4 and patent document 5 as a herbicide for a field crop horticulture as patent herbicide 6 and patent document 7 as a water-soluble herbicide.

As a prior art example using 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide, Patent Document 8 discloses root gall disease and potato lanceus of rapeseeds and crops. It is disclosed that exhibiting excellent control effect against. In addition, the method of raising seedlings of flat land and plants by performing irrigation treatment, such as hydration of an anilide, and soaking treatment, on the root before formulation in Patent Literature 9 is disclosed in Patent Literature 10. A soil disease control agent containing hydroxy-5-methyl-isoxazole as an active ingredient and a soil disease control agent containing the anilide and tetrachloroisophthalonitrile as active ingredients have been reported in Patent Document 11.

On the other hand, Patent Literature 12 reports on the use of an arylsulfonamide derivative as a pesticide.

[Patent Document 1] Japanese Patent Application Laid-Open No. 11-335217

[Patent Document 2] Japanese Patent Publication No. 2003-342109

[Patent Document 3] Japanese Patent Publication No. 2005-137330

[Patent Document 4] Japanese Patent Application Laid-Open No. 54-163814

[Patent Document 5] Japanese Patent Application Laid-Open No. 58-026863

[Patent Document 6] Japanese Patent Application Laid-Open No. 57-38706

[Patent Document 7] Japanese Patent Application Laid-Open No. 57-167907

[Patent Document 8] Japanese Patent Application Laid-Open No. 61-197553

[Patent Document 9] Japanese Patent Application Laid-Open No. 8-71

[Patent Document 10] Japanese Patent Application Laid-Open No. 8-198713

[Patent Document 11] Japanese Patent Application Laid-Open No. 11-292713

[Patent Document 12] US Patent No. 3034955

SUMMARY OF THE INVENTION An object of the present invention is to provide a method for the control of flat and plant diseases, which is stable, has high control effect and high safety, and is free from environmental pollution, against root-knot diseases, which are flat and plant diseases.

As a result of earnestly examining in order to solve the said subject, the present inventors made the agent which contains the strain Barioborax bacterium CGF4526 as an active ingredient against root rot diseases, which are rapeseed plant diseases, such as cabbage and cabbage, which are rape, 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide represented

By contacting a plant containing a as an active ingredient to the rapeseed and plants, to find a control method having a high control effect was completed the present invention.

That is, this invention provides the control method of the rape and plant disease as described in the following [Invention 1]-[Invention 6].

[Invention 1]

A method for controlling rapeseed plant diseases, comprising: contacting a rapeseed plant with an agent comprising Variovorax sp. CGF4526 strain (Variovorax sp. CGF4526) as an active ingredient, and a 2 ', 4-dichloro-α represented by Formula 1 A method for controlling rapeseed and plant diseases, comprising the step of contacting the rapeseed and the plant planting soil with an agent comprising, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide as an active ingredient.

[Invention 2]

Contact with rapeseed and plants is carried out by mixing or irrigation of an agent comprising a strain of Barioborax bacterium CGF4526 as an active ingredient in seedling soil at the time of sowing, or immersing or irrigation in seedlings before planting. The method as described in the invention 1 characterized by the above-mentioned.

[Invention 3]

The contact with the soil was carried out on the soil surface with an agent comprising 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide represented by the formula (1) as an active ingredient. It carries out by spraying or soil-miscible, The method of invention 1 characterized by the above-mentioned.

[Invention 4]

As a method for the control of rapeseed and plant diseases, after mixing or irrigation of a seedling containing Variovorax sp. CGF4526 strain (Variovorax sp. CGF4526) as an active ingredient in seedling soil at the time of sowing, additionally immersing or irrigating the seedlings before planting A process comprising, as an active ingredient, a 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide represented by the formula (1); A method of controlling rape and plant diseases, including a process of spraying or mixing soil.

[Invention 5]

The method according to any one of the inventions 1 to 4, wherein the rapeseed and plant diseases are root gall diseases.

[Invention 6]

The method according to any one of inventions 1 to 5, wherein the rapeseed plant is Chinese cabbage, cabbage, radish, cauliflower, broccoli, bok choy, or sakchae.

As described above, in the method described in Patent Literature 3, when the Barioborax bacteria CGF4526 strain was used alone, the control effect was weak in some cases of severe onset (see Comparative Example). However, surprisingly, by combining the Barioborax bacteria CGF4526 strain and 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide, the control effect is significantly improved. It was found that a high control effect was obtained stably even under severe occurrence. In addition, the term "combining" as used herein does not mix Barioborax bacteria CGF4526 strain and 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide. , "Use together" alone agent is said.

For example, as shown in the comparative example mentioned later, about the root gall disease of a rapeseed plant, Barioborax bacteria CGF4526 strain or 2 ', 4-dichloro- (alpha), (alpha), (alpha)-trifluoro-4'- When nitro-m-toluenesulfonanilides were acted alone, the control effect was small. In addition, when the two agents were mixed, 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide and Barioborax bacteria CGF4526 strains were each controlled. No function as a zero occurs, and the control effect is hardly obtained.

On the other hand, according to patent document 9, the "pulse sulfamide agent" corresponded to 2 ', 4- dichloro- (alpha), (alpha), (trifluoro) 4'-nitro-m-toluene sulfonanilide is mixed with seedling soil. It is disclosed that the treatment is performed, and that there is little control effect. However, in the present invention, by combining the two agents described above, specific improvement, that is, expected when only two agents are applied alone is expected. It was found that there was a synergistic effect far exceeding the effect.

The details of the mechanism are not clear, but it is expected that the antimicrobial activity of the two root roots of bacteria is related.

2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide, and Barioborax bacteria CGF4526 strains have a control effect against the root gall diseases of rape and plants It is known widely from the past. However, 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m- was added to the strain of Barioborax bacteria CGF4526, which had a weak control effect in case of severe onset. By combining toluenesulfonanilide, it is not known to have a markedly high control effect even in the case of severe onset.

In the present invention, the method of controlling the root gall disease of rapeseed and plants can strongly inhibit the onset of root gall disease even when the incidence of the disease is higher than in the prior art, and is significantly higher than the single strain or the agent alone. Has an effect.

In addition, the control method of the present invention is very useful control method because it does not cause environmental pollution and does not cause damage to flat land and plants.

The present invention will be described in detail below.

An object of the present invention is a method for controlling rapeseed and plant diseases, comprising a baculobarax bacterium CGF4526 strain (Variovorax sp. CGF4526) as an active ingredient, and 2 ', 4-dichloro-α, α represented by the formula (1). The agent containing (alpha)-trifluoro-4'-nitro-m-toluenesulfonanilide as an active ingredient (henceforth a "chemical agent N" in this specification) is made to contact a flat plant, It is a method of controlling rape and plant diseases.

First, the CGF4526 strain is described.

The CGF4526 strain was selected from about 7000 bacteria isolated and collected from rice or vegetables, and was selected for antibacterial activity against Fusarium oxysporum and Verticillium daliae, which are filamentous bacteria, and by pot assay tests using Chinese cabbage seedlings. The resulting strain. The strain is deposited in the Patent Biotechnology Depository Center of the Industrial Technology Research Institute, an independent administrative corporation, and obtains the following accession number and is disclosed in Patent Document 3.

Variovorax bacterium CGF4526 strain (Variovorax sp. CGF4526): FERM BP-10160

Next, the chemical agent N is demonstrated. Chemical agent N is a substance already known as an agricultural bactericide, as exemplified in the above patent document. The chemical agent N has antimicrobial ability against various plant pathogens, and has high control ability against root flat diseases of plants, plant stems, etc. In addition, bacteria and the like exhibit antimicrobial activity against Gram-positive bacteria.

With respect to the chemical agent N, for example, it is possible to use a product produced by the method disclosed in Patent Documents 8-11 or commercially available.

The plant which is the object of the plant disease in this invention is flat plants, such as a cabbage, cabbage, a radish, a cauliflower, broccoli, a bok choy, and a litter, but is not limited to these. In addition, as a target of the flat and plant diseases, root-knoll disease, but counterfeit disease, sulphide disease, etc. are also the target disease.

Although the culture method, immobilization, formulation, and preparation method of the bacteria of the CGF4526 strain can be carried out by a known method disclosed in Patent Document 3, specific examples will be described below.

There is no restriction | limiting in particular as long as CGF4526 strain propagates as a medium, As long as the medium contains a suitable carbon source, nitrogen source, and inorganic substance, both a natural medium and a synthetic medium can be used. Examples of the medium include 802 medium, bouillon medium, King's B medium, PS medium, PDB medium, and the like. After culturing at 15-42 ° C., preferably at 28 ° C.-35 ° C. for 10 to 35 hours, the cells are grown and concentrated by centrifugation or a membrane concentrator to remove bacteria. By this operation, the concentration of each cell is usually concentrated at about 1 to 50 x 10 ¹⁰ cfu / ml. Subsequently, a protective agent comprising a saccharide, sodium glutamate, and sodium phosphate buffer is added to the wet cells, followed by vacuum drying. It is preferable to pre-freeze the cells mixed with the protective agent before vacuum drying and to vacuum-dry the cells in the frozen state in order to maintain the viability of the bacteria. In addition, the protective agent may be mixed with the cells in the state of an aqueous solution, or may be mixed in a solid state.

Next, immobilization is demonstrated. Immobilization means the operation of vacuum-drying by adding a protective agent to the cultured bacteria described above. There is no restriction | limiting in particular also about immobilization, As a protective agent used at the time of immobilization, the saccharide which becomes 1 type (s) or 2 or more types of sucrose, fructose, glucose, and sorbitol is used, it mixes with a cell, and vacuum-drying or freeze-vacuum etc. It can carry out by drying by the method of.

Next, formulation is demonstrated. Formulation means the operation which mixes a carrier with respect to the immobilized cultured cell mentioned above, and uses it as a formulation.

The CGF4526 strain may be used as it is after the incubation of the live cells, but since the cells are immobilized, the CGF4526 strain may be mixed with a solid (powder, granules, hydrates), or a liquid carrier to prepare as a preparation. It can adjust suitably.

Examples of the carrier used in the preparation of the preparation include mineral powders such as talc, clay, calcium carbonate and diatomaceous earth, high molecular compounds such as peat moss and polyvinyl alcohol, and natural high molecular compounds such as xanthan rubber and alginic acid. There is this.

Next, the specific bacterial concentration in the CGF4526 strain will be described. The bacterium concentration fluctuates according to its own flat land and plant control method. For example, in the case of contact with seedling soil at the time of sowing, the bacterium concentration is usually 10 ⁵ cfu / g to 10 ¹¹ cfu / g for each strain, but preferably 10 ⁶ cfu / g to 10 ⁹ cfu / g, more preferably 10 ⁷ cfu / g to 10 ⁹ cfu / g.

In addition, the CGF4526 strain can be irrigated by diluting with water to seedling soil as it is. In this case, the bacterium concentration is usually 10 ⁵ cfu / ㎖ ~ 10 ¹¹ cfu / ㎖ for each strain, preferably 10 ⁶ cfu / ㎖ ~ 10 ⁹ cfu / ㎖, more preferably 10 ⁷ cfu / ㎖ ~ 10 ⁹ cfu / ml.

On the other hand, it is also possible to make varioborax bacteria CGF4526 strain into a solid (powder, granulated, hydrated) or liquid formulation, and then contact with seedling soil upon sowing. In this case, the bacterium concentration is usually 10 ⁵ cfu / g to 10 ¹¹ cfu / g in the case of a solid preparation (powder or granules) for each bacterium, but preferably 10 ⁶ cfu / g to 10 ⁹ cfu. / g, more preferably 10 ⁷ cfu / g ~ 10 ⁹ cfu / g.

In addition, in the case of a solid preparation (hydrating agent), it is usually 10 ⁷ cfu / g to 10 ¹² cfu / g, but preferably 10 ⁸ cfu / g to 10 ¹¹ cfu / g, more preferably 10 ⁹ cfu / g to 10 ¹¹ cfu / g.

In addition, about the case where a liquid formulation is contacted with the seedling and the seed | soil at the time of sowing, it can adjust in the same concentration range as the said solid formulation (powder or granules).

In addition, when the CGF4526 strain is prepared as a solid (hydrated, powdered or granulated) or liquid formulation, and then irrigated by diluting with water to seedling soil at the time of sowing, the concentration of the bacteria is usually a solid formulation (powdered or granulated). 10 ⁵ cfu / ml-10 ¹¹ cfu / ml, but preferably 10 ⁶ cfu / ml-10 ⁹ cfu / ml, more preferably 10 ⁷ cfu / ml-10 ⁹ cfu / ml.

In the case of a solid preparation (hydrating agent), it is usually 10 ⁷ cfu / ml-10 ¹² cfu / ml, but preferably 10 ⁸ cfu / ml-10 ¹¹ cfu / ml, more preferably 10 ⁹ cfu / ml- 10 ¹¹ cfu / ml.

In addition, about the case where a liquid formulation is diluted with water and irrigation with the seedling soil at the time of sowing, it can adjust in the same concentration range as the said solid formulation (powder or granules).

Next, the adjustment method of the CGF4526 strain concentration is demonstrated.

First, when the CGF4526 strain is brought into contact with seedling soil at the time of sowing, it is adjusted by contacting the seedling soil with the above-mentioned concentration range.

Next, when the CGF4526 strain is diluted with water in seedling soil at the time of sowing and irrigated, first, the concentration of the CGF4526 strain is diluted with water so as to have the above-mentioned concentration range. After that, the seedlings are irrigated at the time of sowing.

On the other hand, when the CGF4526 strain is prepared as a solid or liquid formulation, the formulation is added and adjusted so as to be in the concentration range described above, and then contacted with the seedling soil at the time of sowing, or diluted with water, and then irrigated with the seedling soil at the time of sowing. do.

The dosage when the chemical agent N is used is explained. The dosage of chemical N depends on the type of pest, soil conditions (moisture, pH), organic matter content, and weather conditions. When chemical agent N is applied as a soil treatment agent, it is usually carried out at 50 g to 500 g / 10 a as an effective amount, preferably 50 g to 400 g / 10 a, more preferably 70 g to 200 g / 10 a. to be.

In addition, the "active ingredient amount" referred to here means the component amount of 2 ', 4- dichloro- (alpha), (alpha), (alpha)-(trifluoro-4'-nitro-m-toluenesulfonanilide only in chemical agent N). For example, the dosage varies greatly depending on the application form (formulation). In that case, those skilled in the art can adjust suitably to become the range of the above-mentioned effective amount.

Next, the control method to the rape and plant disease is demonstrated.

In general, the production of rapeseed and other plants such as Chinese cabbage is seeded by filling the seedlings in the seedling tray. After raising it for 3-5 weeks, it is formalized in a pavement. The formality here means moving a plant from a nursery to a field, and planting it in this way.

As a control method of this invention, the following control methods

i) contacting the CGF4526 strain to rapeseed and plants

ii) contact chemical N with the soil that setstles the plant

Can be mentioned.

First, i) will be described. As a treatment method, the CGF4526 strain can be treated by mixing or irrigation to seedling soil at the time of sowing, or by irrigation to seeds before and after sowing. It is also possible to dilute with water to immerse or irrigate the mock roots before planting in the diluting solution, or to irrigate the seedlings during seedling or just before planting.

For example, the treatment at the time of planting and before planting, that is, mixing or irrigation to seedlings at the time of planting, or immersing or irrigation at the seedlings before planting, is preferred because the root fixation of the bacterium is more efficient. It is one of the suns.

What is necessary is just to mix 1g or more per 1L of soil, and to stir until it becomes uniform, when mixing-processing by seedlings is carried out here.

On the other hand, it is also possible to treat the CGF4526 strain as a solid or liquid formulation and then to treat it by the above-described treatment method.

Next, the processing method of ii) is demonstrated. The chemical agent N is brought into contact with the soil (packing, etc.) subject to formality after i) treatment. That is, it can carry out by spraying on the soil surface, or by mixing with soil (front soil mixing, cropping soil mixing).

The treatment method of i) and ii) is not particularly limited in the number of times of treatment. For example, performing the treatment method of i) a plurality of times is one of the preferred treatment methods because the control effect can be further enhanced. . For example, as shown in Examples described later, after irrigation of CGF4526 strains to seedlings at the time of sowing, additional fertilization to the seedlings before planting is performed a plurality of times, whereby root settlement of the main bacteria is more efficiently achieved. It is mentioned as one of the especially preferable aspect from the side.

As described above, in the present invention, by using the agent containing the CGF4526 strain as an active ingredient and the agent containing the chemical agent N as an active ingredient, high control effects can be exhibited even in soils with high incidence.

Next, although an Example is shown, this invention is not limited by the following example.

In addition, the composition of the medium used in the Example is shown below.

Bouillon medium: 3 g broth, 10 g peptone, 15 g NaCl, 1 L water, pH 7.0

In addition, about chemical agent N, the thing commercially available was used.

Example 1

Control test with Chinese cabbage root disease (pot test)

Using the preparation of the CGF4526 strain, the effect of inhibiting the onset of Chinese cabbage root-knot disease was examined. The preparation of the formulation was prepared by diluting the cultured and dried cells with a carrier (extender) appropriately by the method described above and adjusting the cells to have a bacterial concentration of 2 x 10 ¹⁰ cfu / g.

The formulation was diluted 200-fold and irrigated in soil (128 well seedling trays, 60 cm x 30 cm) sown with Chinese cabbage seeds (variety: kumquat). After that, they were seeded for about three weeks and then planted in contaminated soil. On the other hand, the same irrigation treatment was carried out the day before planting, that is, before planting, and also when planted in contaminated soil.

On the other hand, the chemical agent N was mixed with contaminated soil at a rate of 30 kg / 10a immediately before the cabbage formulation.

The contaminated soil was cabbage root fungus and used field soil mixed with homogenized diseased cabbage roots. After 28 days, the presence or absence of outbreak was examined and the control effect was judged. The results are shown in Table 1.

The incidence of root gall disease was evaluated by calculating the incidence rate, incidence, and control value from the root nodule condition of the root.

Root incidence index 0; Sound, 1; Confirmation of the attachment of root nodules to roots of roots, 2; Confirmation of attachment of many root nodules to the roots, or attachment of root nodules to the main roots, 3; Confirmation or death of large root nodules on the roots.

Incidence rate (%) = 100 × {(weeks onset) ÷ (total weeks)}

Incidence = 100 × {∑ (value of exponent) × (number of individuals corresponding to each exponent)} ÷ {3 × (parameter of survey)})

Control value = 100 × {(degree of incidence in untreated)-(degree of indigestion)} ÷ (degree of untreated)

As a result, in the case of severe onset, when used in combination with the bacteriobacterium bacterium CGF4526 strain and the chemical | medical agent N, the high control value was obtained compared with the comparative example 1 and the comparative example 2 mentioned later.

[Comparative Example 1] Control Test for Chinese Cabbage Root Kick Disease (Port Test)

The same procedure as in Example 1 was carried out except that the strain Varioborax bacteria CGF4526 was used alone. The homogeneity of the suspension was also performed at 1 × 10 ⁸ cfu / ml.

The incidence of root gall disease was evaluated in the same manner as in Example 1 by calculating the incidence rate, the incidence rate, and the control value from the root gum state of the root portion.

Comparative Example 2 Test for Controlling Chinese Cabbage Root Gland Disease (Port Test)

An example of using the chemical agent N alone is shown. The ratio applied to contaminated soil was also performed at 30 kg / 10 a.

The incidence of root gall disease was evaluated in the same manner as in Example 1 by calculating the incidence rate, the incidence rate, and the control value from the root gum state of the root portion.

As described above, as shown in Tables 2 and 3, it can be seen that when the CGF4526 strain and the chemical agent N are used alone, the control effect is weak as compared with Example 1.

[Example 2]

Control test with Chinese cabbage root disease (pot test)

Using the preparation of the CGF4526 strain, the effect of inhibiting the onset of Chinese cabbage root-knot disease was examined. The preparation of the formulation was prepared by diluting the cultured and dried cells with a carrier (extender) appropriately by the method described above and adjusting the cells to have a bacterial concentration of 2 x 10 ¹⁰ cfu / g. The formulation was diluted 200-fold and irrigated to the soil sown cabbage seeds (breed: kumquat). After that, they were seeded for about three weeks and then planted in contaminated soil. The same irrigation treatment was also carried out the day before planting, that is, before planting and planting in contaminated soil. The contaminated soil was a field soil mixed with homogenized cabbage roots (attached to the roots), which were diseased as cabbage root fungi. On the other hand, the chemical agent N was mixed with contaminated soil at a rate of 30 kg / 10a immediately before the cabbage formulation. After 27 days, the presence or absence of outbreak was examined and the control effect was judged. The results are shown in Table 4.

The incidence of root gall disease was evaluated in the same manner as in Example 1 by calculating the incidence rate, the incidence rate, and the control value from the root gum state of the root portion.

As a result, when inoculating by combining the strain of Barioborax bacteria CGF4526 and the chemical agent N, a very high control value was obtained in comparison with Comparative Examples 3 and 4 described later.

Comparative Example 3 Control Test on Chinese Cabbage Root Kick Disease (Port Test)

The same procedure as in Example 2 was conducted except that the strain Varioborax bacteria CGF4526 was used alone. The bacterium concentration of the suspension of the strain Varioborax bacteria CGF4526 was also performed at 1 × 10 ⁸ cfu / ml.

The incidence of root gall disease was evaluated in the same manner as in Example 1 by calculating the incidence rate, the incidence rate, and the control value from the root gum state of the root portion.

Comparative Example 4 Control Test for Chinese Cabbage Root Gland Disease (Port Test)

It carried out similarly to Example 2 except having used chemical agent N independently. The ratio applied to contaminated soil was also performed at 30 kg / 10 a.

The incidence of root gall disease was evaluated in the same manner as in Example 1 by calculating the incidence rate, the incidence rate, and the control value from the root beak condition.

As mentioned above, as shown in Table 5 and Table 6, when used alone, it turns out that a control effect is weak compared with Example 2.

Example 3

Control test to cabbage root gall disease (packaging test)

Using the preparation of the CGF4526 strain, the effect of inhibiting the onset of Chinese cabbage root-knot disease was examined. The preparation of the formulation was prepared by diluting the cultured and dried cells with a carrier (extender) appropriately by the method described above, and adjusting the cells to have a concentration of 2 x 10 ¹⁰ cfu / g. The formulation was diluted 200-fold and irrigated to the soil sown cabbage seeds (breed: kumquat). Then, after seeding for about three weeks, the cabbage roots (attached to the roots) affected by the cabbage root fungus were homogenized and transplanted into the mixed field. In addition, the same irrigation treatment was carried out the day before the establishment, that is, before the establishment, and then the case was planted in contaminated packaging. On the other hand, the chemical agent N was mixed with contaminated soil at a rate of 30 kg / 10a immediately before the cabbage formulation. After 70 days, the presence or absence of outbreak was examined and the control effect was judged. The results are shown in Table 7.

The incidence of root gall disease was evaluated in the same manner as in Example 1 by calculating the incidence rate, the incidence rate, and the control value from the root gum state of the root portion.

As a result, when the inoculation was inoculated with a strain of Barioborax bacteria CGF4526 and the chemical agent N, a high control value was obtained in comparison with Comparative Examples 5 and 6 described later.

Comparative Example 5 Control Test on Chinese Cabbage Root Gland Disease (Packaging Test)

The same procedure as in Example 2 was conducted except that the strain Varioborax bacteria CGF4526 was used alone. The bacterium concentration of the suspension of the strain Varioborax bacteria CGF4526 was also performed at 1 × 10 ⁸ cfu / ml. After 70 days, the presence or absence of outbreak was examined and the control effect was judged. The results are shown in Table 8.

The incidence of root gall disease was evaluated in the same manner as in Example 1 by calculating the incidence rate, the incidence rate, and the control value from the root gum state of the root portion.

Comparative Example 6 Control Test for Chinese Cabbage Root Gland Disease (Packaging Test)

It carried out similarly to Example 2 except having used chemical agent N independently. The ratio applied to contaminated soil was also performed at 30 kg / 10 a. After 70 days, the presence or absence of outbreak was examined and the control effect was judged. The results are shown in Table 9.

The incidence of root gall disease was evaluated in the same manner as in Example 1 by calculating the incidence rate, the incidence rate, and the control value from the root gum state of the root portion.

As mentioned above, as shown in Table 8 and Table 9, when used alone, it turns out that the control effect is weak compared with Example 3.

1 compares the control values in Examples and Comparative Examples. Here, A represents a strain of Barioborax bacteria CGF4526, B represents a chemical agent N.

Claims (6)

A method for controlling rapeseed and plant diseases, comprising the steps of contacting a plant with a rapeseed comprising a Bariovorax bacterium CGF4526 strain (Variovorax sp. CGF4526) as an active ingredient and a plant represented by the formula (1). Dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide [Formula 1]

A method for controlling rapeseed and plant diseases, comprising the step of contacting the rapeseed and the soil to form a plant. The method of claim 1, A method of contacting a rapeseed and a plant is carried out by mixing or irrigation of an agent comprising a strain of Barioborax bacteria CGF4526 as an active ingredient, by immersion or irrigation in seedling soil at the time of sowing, or by immersion or irrigation in the hair before planting. The method of claim 1, The contact with the soil was carried out on the soil surface with an agent comprising 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide represented by the formula (1) as an active ingredient. By spraying or soil admixture. As a method for the control of rapeseed and plant diseases, after mixing or irrigation of an agent comprising Variovorax sp. CGF4526 strain (Variovorax sp. Process, and 2 ', 4-dichloro-α, α, α-trifluoro-4'-nitro-m-toluenesulfonanilide represented by the formula (1) [Formula 1]

A method for controlling rapeseed and plant diseases comprising the step of spraying or mixing the soil with the agent comprising the above as an active ingredient in the soil for formulating the rapeseed and plants. The method according to any one of claims 1 to 4, Rapeseed plant disease characterized in that the root-knot disease. 6. The method according to any one of claims 1 to 5, How rapeseed plants are cabbage, cabbage, radish, cauliflower, broccoli, bok choy, or lichen.

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