KR20100041363A - A composition comprising fruits of cudrania tricuspidata for immunopotentiating - Google Patents

Abstract

PURPOSE: A composition for enhancing immunity containing Cudrania tricuspidata fruit extract is provided to promote splenocytes and macrophages and ensure immunity enhancement. CONSTITUTION: A composition for enhancing immunity contains Cudrania tricuspidata fruit extraction as an active ingredient. The Cudrania tricuspidata extract is isolated using water, low alcohol of C1-C4, or mixture solvent thereof. The low alcohol is ethanol or methanol. The Cudrania tricuspidata fruit is isolated through hot water extraction, ultrasonic wave extraction, or reflux extraction. An agent for preventing and treating diseases caused by immunity deterioration contains the Cudrania tricuspidata fruit extract as an active ingredient. The diseases caused by immunity deterioration are infectious diseases, inflammatory diseases, allergic disease such as atopy, chronic fatigue or cancer.

Description

A composition containing fruits of Cudrania tricuspidata for immunopotentiating}

The present invention is a composition for strengthening immune function containing Cudrania tricuspidata fruit extract, health food for enhancing immune function containing Cudrania fruit extract, and prevention of diseases caused by lowering immune function containing Cudrania fruit extract And to therapeutic agents.

Cudrania tricuspidata (Carr.) Bureau ex Lavallee is also called old mulberry tree, good bark tree, barberry, etc. and is classified as deciduous broad-leaved subtree or shrub of Moraceae. It is a plant of the same type as the mulberry tree, and its origin is Korea, and the leaves are revived, divided into 2-3 pieces, and the edges are flat and ovate. The branching leaves are blunt-headed, the edges are acute, broad-headed, 6-10 cm long, 3-6 cm wide, with fine hairs on the surface, and villi on the back. The petioles are 15-25 mm long and hairy. Leaf shape is bigger than persimmon leaf when the growth place is fertile, and there are fewer cornucopia, but growing in rock cracks or barren slopes has small leaf, severe cornea, and leaf tip extends like tail. Fruits are round, with a diameter of 2.5 cm in diameter, fleshy, mature in red in September-October, and achenes are 5 mm long, black. The flesh is sweet and edible (National Biotechnology Information System, http://www.nature.go.kr/plant/plantGuide/). Euchrestaflavanone B and euchrestaflavanone C have been reported as antimicrobial actives of Cucurbita japonica (Lee Hospital, Kang Sook Park, and Ki Hoon Park. 270-273), but there have been reports of lipid peroxide-reducing activities such as kaempferol, kaempferol 7-O-β-D-glucoside, norarthocarpetin, and arthocarpesin isolated from leaves and necks Effects of Fractions, Flavonoid Compounds, and Flavonoid Compounds on Fruit, Lipid Peroxide Contents in Rats (1995) 26: 377-384) No effective activity has been reported on the fruit.

Representative methods of verifying immune activation are macrophage proliferation effects (Friedl R, Moeslinger T, Kopp B, and Spiecke-rmann PG.Stimulation of nitric oxide synthesis by the aqueous extract of Panax ginsena root in Raw 264.7 cells.British Journal of pharma -cology, 2001; 134: 1663-1670.) and the proliferative effects of splenocytes were selected. NO is a marker of macrophage activation. NO produced in macrophages acts to eliminate invading pathogens and activate basic host defenses (Farrell AJ, Blake DR. Nitric oxide. Annals of the Rheumatic Dieases. 1996; 55: 7-20.). Particularly, when Thl and Th2 are divided in the ThO state during the differentiation of Th cells, it is known to have a function of helping to differentiate into Thl. In addition, cytotoxic activity of killing cancer cells, parasites or bacteria among leukocyte activity has been reported to be released by the release of NO (Nathan C. Nitric oxide as a secretory product of mammalian cells.FASEB Journal. 1992; 6: 3051-3064) .). In addition, NO induced immunomodulating between T-cell and endothelial cells (Bingisser RM, Holt PG Immunomodulating mechanism in the lower respiratory tract; nitric oxide mediated intraction between alveolar macrophage, epithelial cell, and T-cell, SWISS Medical Weekly. 2001; 131; 171-179.) It is known that there is a potent platelet aggregation inhibitory effect.

In this regard, the inventors of the present invention, Cucumberberry fruit extract promotes the proliferation of splenocytes and macrophages, induces the production of NO, and exhibits an excellent effect on the enhancement of immune sensitization in mice. By confirming that the present invention was completed.

An object of the present invention is a composition for enhancing the immune function containing Cudrania tricuspidata fruit extract as an active ingredient, health food for enhancing the immune function containing the Cudrania fruit extract as an active ingredient, and containing Cucumber fruit extract It is to provide a prophylactic and therapeutic agent for diseases caused by impaired immune function.

The present invention provides a composition for enhancing immune function containing Cudrania tricuspidata fruit extract as an active ingredient.

In another aspect, the present invention provides a health food for enhancing immune function containing Cucumber fruit extract as an active ingredient.

The present invention also provides a prophylactic and therapeutic agent for diseases caused by a decrease in immune function, which contains Cucumber fruit extract as an active ingredient.

In addition, the present invention provides a method for the prevention and treatment of diseases caused by impaired immune function, comprising the step of administering a pharmaceutically effective amount of Cudrania japonica extract to an individual.

Since the extract of the Koji mulberry fruit of the present invention increases the growth of splenocytes and macrophages in mice, increases the production of NO, and shows an excellent effect on the enhancement of immune sensitization in mice, it is effective in enhancing immune function. It is useful for use as a composition for strengthening functions, health foods for enhancing immune function, and as an agent for preventing and treating diseases caused by reduced immune function.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for enhancing immune function containing Cudrania tricuspidata fruit extract as an active ingredient.

The extract is preferably extracted using water, alcohol or a mixture thereof as a solvent, but is not limited thereto. It is preferable that the lower alcohol is a C ₁ to C ₄ lower alcohol. It is preferable to use about 5 to 20 times, preferably about 10 times, water, a lower alcohol, or a mixture thereof as an extractant to the Cucumber fruit, and the lower alcohol is preferably methanol or ethanol, The mixture of water and lower alcohol is preferably 10 to 90% methanol or ethanol mixture, but is not limited thereto. In addition, the extraction is preferably performed at room temperature for about 1 to 24 hours, preferably 4 to 15 hours, most preferably 1 to 2 hours, but is not limited thereto. The extraction method is preferably any one selected from the group consisting of hot water extraction, cold needle extraction, reflux cooling extraction, ultrasonic extraction and steam extraction, but is not limited thereto. By performing lyophilization after extraction, it is possible to obtain the Koji mulberry fruit extract of the present invention.

The present inventors investigated the effect of the extract of Koji mulberry fruit extract as described above on the growth of spleen cells, macrophages of mice. As a result, it induces the proliferation of splenocytes (see Table 2), showed an excellent proliferation effect even in macrophages, it was confirmed to be superior to the positive control (see FIGS. 1 to 3).

The present inventors then investigated the effect of the kkujimi fruit extract on NO production. As a result, it was confirmed that the production of NO also effective in enhancing the immune (see Fig. 4 and Table 3). In addition, it was also found that the effect of enhancing immune sensitization in mice was also excellent (see FIG. 5).

Therefore, the Quercus mulberry fruit extract of the present invention can be efficiently used in the composition for enhancing immune function.

The composition for enhancing immune function of the present invention may be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, oral formulations, external preparations, suppositories, and sterile injectable solutions. Carriers, excipients and diluents that may be included in the compositions of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose ( It is prepared by mixing sucrose or lactose and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. .

Formulations for parenteral administration include sterile aqueous solutions, solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, suppositories, injections. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

In addition, the present invention provides a health food for enhancing immune function containing Cudrania tricuspidata fruit extract as an active ingredient.

Examples of the foods to which the Cucumber fruit extract may be added include, for example, various foods, dairy products, beverages, gums, teas, vitamin single agents, health supplements, and the like, powder, granules, tablets, capsules, or beverages. Can be used as

It may also be added to foods or beverages for the purpose of enhancing immune function. At this time, the amount of the extract in the food or beverage can be added in 0.01 to 15% by weight of the total food weight, the health beverage composition is added in a ratio of 0.02 to 5 g, preferably 0.3 to 1 g based on 100 ml Can be.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for containing the Cucumberberry extract as essential ingredients in the indicated proportions, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. Can be. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the ziddonia fruit extract of the present invention is a variety of nutrients, vitamins, minerals (electrolytes), synthetic and natural flavors, such as flavoring agents, coloring and neutralizing agents (cheese, chocolate, etc.), pectic acid and salts thereof, Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the Cudrania fruit extract of the present invention may contain a fruit flesh for the production of natural fruit juice and fruit juice beverage and vegetable beverage. These components can be used independently or in combination. The ratio of such additives is not so important but is generally selected from the range of 0.1 to about 20 parts by weight per 100 parts by weight of the kkujiwan fruit of the present invention.

In addition, the present invention provides a preventive and therapeutic agent for diseases caused by reduced immune function containing Cudrania tricuspidata fruit extract as an active ingredient.

The disease caused by the reduced immune function is preferably any one selected from the group consisting of infectious diseases such as colds and inflammatory diseases, allergic diseases such as atopy, chronic fatigue, and cancer, but is not limited thereto. All diseases resulting from a known decrease in immune function are included in the present invention. Preferred dosages of Cucumberberry extract of the present invention vary depending on the condition and weight of the patient, the extent of disease, drug form, route of administration and duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the Cudrania japonica fruit extract of the present invention is preferably administered at 0.0001 to 500 mg / kg, preferably at 0.001 to 500 mg / kg. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, the present invention provides a method for the prevention and treatment of diseases caused by impaired immune function comprising the step of administering a pharmaceutically effective amount of kkujipan fruit extract to a subject.

In the above method, the Quercus mulberry fruit extract of the present invention can be administered by various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples and Formulation Examples.

However, the following Examples, Experimental Examples, and Formulation Examples specifically illustrate the present invention, and the content of the present invention is not limited to Examples, Experimental Examples, and Formulation Examples.

<Example 1> Koji mulberry fruit ( Cudraniae fructus A) extract manufacturer

<1-1> Preparation of Cucurbita Fruit Extract

Cuji mulberry fruit (from China) was purchased from the medicinal herb (Baekje-Dang, Daejeon) and used. An extraction solvent corresponding to 10 times (w / v) of the medicinal herb was used, and three extraction solutions were obtained by using an ultrasonic extraction method and a reflux extraction method using hot water extraction method, 70% ethanol as a solvent as an extraction method. Each extract was concentrated under reduced pressure and lyophilized to prepare an extract. Different yields for each extraction method are shown in Table 1 below.

Yield of Extracts According to Cucumber Fruit Extraction Method sign Extraction solvent Way Yield (%) W Deionized distilled water Hot water extraction, heating time 90 minutes 12.70 ES 70% ethanol Ultrasonic Extraction, Ultrasonication 90 minutes 8.94 ER 70% ethanol 90 minutes of reflux extraction, heating and reflux 12.36

Experimental Example 1 Investigation of Cell Proliferation Effect of Mouse Spleen Cells

Seven-week-old male C57BL / 6 mice (Korean Biolink, Chungbuk Negative) were extracted after the cervical spine bone. The extracted spleens were washed with HBSS (Hank's Balanced Salts Modified.Gbico BRL.), Centrifuged and separated to remove red blood cells with RBC lysing buffer (Sigma), followed by 10% FBS (fetal bovine serum, Gibco BRL. ) And penicillin (penicillin, Gibco BRL.) And streptomycin (streptomycin, Gibco BRL.) And suspended in RPMI 1640 (Hyclone) medium containing and cultured at 5% CO ₂ , 37 ℃. Searching for the effects of Cudrania fruit hydrothermal extract (W), Cudrania fruit 70% ethanol ultrasonic extract (ES) and Cudrania fruit 70% ethanol reflux extract (ER) prepared in <Example 1> on spleen cell proliferation The spleen cells separated and cultured in a 96-well plate were divided into 5 × 10 ⁵ cells / well, and the Cucumber Fruit Extract (Hydrogen Extract (W), 70% Ethanol Ultrasonic Extract (ES) and 70% Ethanol) Reflux extract (ER)) was added dissolved in PBS at a concentration of 2 × 10 ⁻³ to 1 × 10 ⁰ mg / ml. As a comparison material for the cell proliferation effect, ConA (Concanavalin A, Sigma Co., USA) and LPS (lipopolysaccharide, Sigma Co., USA) were used as 2 μg / ml, respectively. After 48 hours of incubation, add tetrazolium salt CCK-8 kit (Cell Counting Kit-8, Dojindo Laboratories, Tokyo, Japan) solution, and incubate for 4 hours more. Absorbance at 450 nm was measured. The ratio of the absorbance of drug addition wells to the absorbance of control wells was calculated in% and the average value of several wells was used. The calculation is as follows.

As a result, the growth of splenocytes when treated with 2.5 mg / ml of dry medicinal herbs in the emergency cells isolated from mice was 1.8 times, 2.2 and 2.2 times, respectively. 2x, increased 2.3 times. This resulted in an increase of 150 ± 2.70 and 198.22 ± 0.93% of the control group when 2 μg / ml of LPS and ConA were used as the positive control group. It can be seen that (Table 2).

Splenocyte Proliferation Effect of Cucumber Fruit Extract in Mouse Spleen Cells Ctrl (%) W ES ER 100 ± 0.95 180.77 ± 10.66 219.56 ± 14.77 233.11 ± 12.52

Experimental Example 2 Investigation of Cell Proliferation and NO (nitric Oxide) Formation in Mouse Macrophage Cell Line

<2-1> Investigation of Cell Proliferation in Mouse Macrophage Cell Line

Raw 264.7 cell line, a Murin macrophage cell line, was dispensed at 5 × 10 ³ cells / well in a 96-well plate, and Cucumber Fruit Extract (Hydrogen Extract (W), 70% Ethanol Ultrasonic Extract (ES) and 70) % Ethanol reflux extract (ER)) was treated at a concentration of 1 × 10 ⁻³ to 1 × 10 ⁰ mg / ml. As a comparison material, ConA (Sigma Co., USA) and LPS (Sigma Co., USA) were used at 2 μg / ml, respectively. After 48 hours of incubation, add tetrazolium salt CCK-8 kit (Cell Counting Kit-8, Dojindo Laboratories, Tokyo, Japan) solution, and incubate for 4 hours more. Absorbance at 450 nm was measured. The ratio of the absorbance of drug addition wells to the absorbance of control wells was calculated in% and the average value of several wells was used. The calculation is as follows.

As a result, the zirconia fruit hot water extract (W) and 70% ethanol extract (ES and ER) were all dried medicinal equivalents at 50 μg / ml concentration, at least 150% of the control group and 200% of the control group at 2500 μg / ml concentration. It was shown that the cell proliferation effect is excellent and to promote the proliferation of RAW264.7 cells in a concentration-dependent range within the concentration range of 50 ~ 2500 μg / ml (Fig. 1 to 3).

<2-2> NO Production Effect of Mouse Macrophage Cell Line

Raw 264.7 cells were aliquoted into 2.5 × 10 ⁵ cells / well in 48-well plates, incubated in CO ₂ incubator for 24 hours, and treated with LPS by concentration as a positive control. The drug treatment group was treated with 0.2, 2, 20, 200, 2000 μg / ml of Cucurbita fruit extract (hot water extract (W), 70% ethanol ultrasonic extract (ES) and 70% ethanol reflux extract (ER)) and 18 After time incubation, the supernatant was separated and NO production was measured. NO production was reacted using Griess reagent (Promega, USA) and the absorbance was measured at 535 nm.

As a result, the extract of Koji berries was treated to 5000 μg / ml concentration RAW 264.7 cells with dry medicinal equivalents, and the amount of NO produced by collecting the supernatant was investigated. (ES) showed no NO production promoting effect in 111% and 100% of the control group, respectively, but reflux extract (ER) of 70% ethanol was found to promote NO production of large cells in 146% of the control group (Fig. 4 and table). 3).

Ctrl (μM) LPS W ES ER 0.87 ± 0.14 8.07 ± 0.69 0.97 ± 0.04 0.87 ± 0.00 1.27 ± 0.14

Example 3 In Vivo Immunity Enhancement Effect of Cucumber Fruit Extract

<3-1> Immunosensitization and Drug Administration

Ovalbumin (Imject Ovalbumin, Thermo Scientific) + Aluminum (Aluminum hydroxide Gel, Sigma-Aldrich) (100 μg + 200 μg / 100 μl) was intraperitoneally administered to a 6-week-old C57BL / 6 mouse. Sensitized (day 0). After 10 days of administration, ovalbumin + aluminum was boosted by dressing (day 10). Cuji mulberry fruit extract prepared in the above example was administered at 5 g / kg / day as a dry medicinal equivalent amount from 0 to 20 days compared the efficacy according to the extraction method. The control group, OVA group and OVA + Al group were orally administered the corresponding amount of distilled water. 20 days after the start of animal experiments, the abdomen was incision under ether anesthesia, blood was collected from posterior vena cava, and the spleen was extracted and used for subsequent experiments.

<3-2> Proliferation of Splenocytes for Search for Mitogen Effect

After administration of Cudrania fruit extract for 20 days, the spleens extracted by each group were washed with HBSS (Hank's Balanced Salts Modified.Gbico BRL.), And then separated by centrifugation to remove red blood cells with RBC lysis buffer (Sigma). 10% FBS (fetal bovine serum, Gibco BRL.), Penicillin and streptomycin containing RPMI 1640 (Hyclone) medium containing was incubated at 5% CO ₂ , 37 ℃. To detect mitogen effects, splenocytes isolated and cultured in 96-well plates were dispensed at 5 × 10 ⁵ cells / well, PBS in control wells, LPS (10 μg / ml) in other wells, ConA (4 μg / ml), OVA (10 μg / ml) were treated. After culturing for 2 days, the cell proliferation was measured by CCK-8 kit, which is tetrazolium salt.

As a result, the C57 BL / 6 mouse, which was sensitized with OVA + Aluminium (100 μg + 200 μg / 100 μl) twice at 10 days intervals, was used as a dry medicinal equivalent with 5 g / kg / day As a result of separating oral spleen after 3 weeks and confirming the cleavage-induced effect, the cleavage-inducing effect of the control group (W) was higher than the control group OVA (Fig. 5).

Through the above results, both the hot water extract and the 70% ethanol extract of the Koji mulberry fruit of the present invention have excellent effects on the cell proliferation of mouse splenocytes, the proliferation of macrophages, the promotion of NO production of macrophages, and the enhancement of immune sensitization in mice. As shown, the present invention suggests that the Cudrania tree can be used for immune activation and immune function enhancement.

Examples of formulations for the composition of the present invention are illustrated below.

Preparation Example 1 Preparation of Pharmaceutical Formulation

<1-1> Preparation of Powder

Cudrania fruit water extract 2 g of the present invention

1 g lactose

After mixing the above components, the airtight cloth was filled to prepare a powder.

<1-2> Preparation of Tablet

Cudrania fruit 70% ethanol extract (ES) according to the invention 100 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

<1-3> Preparation of Capsule

Cudrania fruit 70% ethanol extract of the present invention (ER) 100 mg

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

<1-4> Preparation of Injection Solution

Cudrania fruit water extract 10 ㎍ / ㎖ according to the present invention

Dilute hydrochloric acid BP until pH 3.5

Injectable sodium chloride BP up to 1 ml

Dissolve the wood mushroom mycelium powder according to the present invention in an appropriate volume of sodium chloride BP for injection, adjust the pH of the resulting solution to pH 3.5 with dilute hydrochloric acid BP, and adjust the volume with sodium chloride BP for injection and sufficiently Mixed. The solution was filled into a 5 ml Type I ampoule made of clear glass, encapsulated under an upper grid of air by dissolving the glass, and sterilized by autoclaving at 120 ° C. for at least 15 minutes to prepare an injection solution.

Preparation Example 2 Preparation of Food

Foods containing Cucumber fruit extract of the present invention according to the present invention were prepared as follows.

<2-1> Preparation of Flour Food

0.1 ~ 10.0 parts by weight of the extract of Kokjiberry fruit water of the present invention was added to flour, and bread, cake, cookies, crackers and noodles were prepared in a conventional manner using this mixture to prepare foods for health promotion.

<2-2> Preparation of Soups and Gravys

0.1 ~ 1.0 parts by weight of the extract of Cudrania fruit water of the present invention was added to soup and gravy to prepare meat products for health promotion, soup and gravy of noodles in a conventional manner.

<2-3> Preparation of Ground Beef

10 parts by weight of the extract of Kokji berry fruit water of the present invention was added to the ground beef to prepare ground beef for health promotion in a conventional manner.

<2-4> Production of Dairy Products

0.1 ~ 1.0 parts by weight of the extract of Cudrania fruit water of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared in a conventional manner using the milk.

<2-5> Preparation of Wire

Brown rice, barley, glutinous rice, and yulmu were alphad by a known method, and then dried and roasted to prepare a powder having a particle size of 60 mesh.

Black beans, black sesame seeds, and perilla were also steamed and dried by a known method, and then ground to a powder having a particle size of 60 mesh.

The dried Cucumber fruit water extract of the present invention was decompressed and concentrated in a vacuum concentrator, dried by spraying and a hot air dryer, and then pulverized to 60 mesh in a particle size with a grinder to obtain a dry powder.

The grains, seeds and the mycelium mycelium mycelium powder according to the present invention were prepared in the following ratio to prepare a conventional method.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight perilla, 8 parts by weight black beans, 7 parts by weight black sesame seeds),

Cudrania fruit water extract of the present invention (1 part by weight),

Ganoderma lucidum (0.5 parts by weight),

Foxglove (0.5 part by weight)

Preparation Example 3 Preparation of Beverage

Drinks containing the extract of the Koji fruit tree of the present invention was prepared as follows.

<3-1> Preparation of Health Beverage

Instant sterilization by homogeneously mixing subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and the extract of Cucurbita fruit according to the present invention. After this, it was packaged in a small packaging container such as glass bottles, plastic bottles to prepare a healthy beverage.

<3-2> Preparation of Vegetable Juice

0.5 g of Cudrania fruit water extract of the present invention was added to 1,000 ml of a vegetable juice such as tomato or carrot to prepare a vegetable juice for health promotion in a conventional manner.

<3-3> Preparation of Fruit Juice

0.1 g of Cudrania fruit water extract of the present invention was added to 1,000 ml of fruit such as apple or grape to prepare fruit juice for health promotion in a conventional manner.

Since the extract of Cudrania japonica of the present invention has an immune activation and augmentation effect, the composition for enhancing immune function, health food for enhancing immune function, and allergic diseases such as infectious diseases and inflammatory diseases, atopy, chronic fatigue, and cancer It can be usefully used as a prophylactic and therapeutic agent for diseases caused by reduced function.

Figure 1 is a graph showing the proliferation effect of macrophages according to the treatment of kkujija fruit hot water extract (W) of the present invention in mouse macrophages.

Figure 2 is a graph showing the proliferation effect of macrophages according to the treatment of the Cudrania fruit 70% ethanol ultrasonic extract (ES) of the present invention in mouse macrophages.

Figure 3 is a graph showing the proliferation effect of macrophages according to the treatment of kkujija fruit 70% ethanol reflux extract (ER) of the present invention in mouse macrophages.

Figure 4 is a graph showing the NO production inducing effect according to the treatment of Koji mulberry fruit extract of the present invention in mouse macrophage line.

FIG. 5 is a graph showing the mitogen effect of the Fiji mulberry fruit hydrothermal extract in OVA + Al sensitized mice.

Claims (12)

Composition for enhancing immune function containing Cudrania tricuspidata fruit extract as an active ingredient. The composition of claim 1, wherein the extract is extracted using water, C ₁ to C ₄ lower alcohols, or a mixture thereof as a solvent. 3. The composition for enhancing immune function according to claim 2, wherein the lower alcohol is ethanol or methanol. The composition of claim 1, wherein the kkujimi fruit is extracted by any one method selected from the group consisting of hot water extraction, ultrasonic extraction or reflux extraction. Health food for enhancing immune function, containing Cudrania tricuspidata fruit extract as an active ingredient. The health food for enhancing immune function according to claim 5, wherein the extract is extracted using water, ethanol or a mixture thereof as a solvent. Cudrania tricuspidata ( Cudrania tricuspidata ) A preventive and therapeutic agent for diseases caused by impaired immune function containing the extract as an active ingredient. 8. The immune function according to claim 7, wherein the disease caused by the reduced immune function is any one selected from the group consisting of infectious diseases such as colds and inflammatory diseases, allergic diseases such as atopy, chronic fatigue, and cancer. Preventive and therapeutic agents for diseases caused by deterioration. A pharmaceutically effective amount of Cudrania tricuspidata fruit extract is administered to an individual suffering from a disease caused by a decreased immune function. 10. The immune function according to claim 9, wherein the disease caused by lowering immune function is any one selected from the group consisting of infectious diseases such as colds and inflammatory diseases, allergic diseases such as atopy, chronic fatigue, and cancer. Methods of treating diseases caused by degradation. A method for preventing disease caused by impaired immune function, comprising administering to a subject a pharmaceutically effective amount of Cudrania tricuspidata fruit extract. 12. The immune function according to claim 11, wherein the disease caused by lowering immune function is any one selected from the group consisting of infectious diseases such as colds and inflammatory diseases, allergic diseases such as atopy, chronic fatigue, and cancer. Prevention method for diseases due to degradation.

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