KR101033003B1 - A composition comprising Broussonetiae Fructus for immunopotentiating - Google Patents

Abstract

The present invention relates to a composition for enhancing immune function containing low fruit extract, specifically, the low fruit extract of the present invention promotes the growth of splenocytes and macrophages of mice and promotes the production of NO, while also immunosensitizing in mice Since it shows an excellent effect on the enhancement of, the low fruit fruit extract of the present invention was confirmed that the immune activation and enhancer effect, the low fruit extract of the present invention is a composition for enhancing immune function, health food for enhancing immune function and immune function It can be usefully used as a preventive and therapeutic agent for diseases caused by deterioration.

Low fat, strengthened immune function

Description

A composition comprising Broussonetiae Fructus for immunopotentiating}

The present invention relates to a composition for enhancing immune function containing low fruiting extract, a health food for enhancing immune function containing low fruiting extract, and an agent for preventing and treating diseases caused by lowering immune function containing low fruiting extract.

Broussonetiae Fructus is the fruit of Broussonetia kazinoki SIEB. Is greyish brown, about 3 m high. The leaves are ovate or oblong in shape, with sharp teeth on the edges, and are torn in the shape of the insect as eaten. Flowers are reddish purple and discharged in the form of male to female in May-June. Fruits are nucleus spherical and mature in September. The mulberry is collected from the oriental medicine in the summer and autumn together with young branches and leaves. Leaves are regenerated, egg-shaped or egg-shaped oval, long apex, round or subcardiac, 5-20 cm long, with sharp ends, sometimes deeply split, with sharp teeth on the edges. Young trees have 2 to 3 missing lobes. The surface is rough and the back is initially hairy. The petiole is 1-2 cm long, with curly hair, but gradually disappears. The fruit of the mulberry, the core family is spherical (편 球形), the collecting fruit (聚合 果) is spherical and ripens in September. Surgical blood is thick with fleshy, fleshy, ripened in red, so it is similar to strawberry and has granular protrusions on inner skin. Fruit is called low fruit (자 子), National species knowledge information system, http://www.nature.go.kr/plant/plantGuide/. Low-chambered rooms are affected by tonic, ripening (healing recovery), nourishing tonic, nominal, intertreatment, ileal lymphadenitis, vulgaris, abdominal pain, diuresis, lack of ripening, rheumatism, edema, isopathy, bruises, dermatitis It is known to be effective.

A representative method of verifying immune activation is the macrophage proliferation effect (Friedl R, Moeslinger T, Kopp B, and Spiecke-rmann PG.Stimulation of nitric oxide synthesis by the aqueous extract of Panax ginsena root in Raw 264.7 cells.British Journal of pharma -cology, 2001; 134: 1663-1670.) and the proliferative effects of splenocytes were selected. NO is a marker of macrophage activation. NO produced in macrophages acts to eliminate invading pathogens and activate basic host defenses (Farrell AJ, Blake DR. Nitric oxide. Annals of the Rheumatic Dieases. 1996; 55: 7-20.). Particularly, when Thl and Th2 are divided in the ThO state during the differentiation of Th cells, it is known to have a function of helping to differentiate into Thl. In addition, cytotoxic activity of killing cancer cells, parasites or bacteria among leukocyte activity has been reported to be released by the release of NO (Nathan C. Nitric oxide as a secretory product of mammalian cells.FASEB Journal. 1992; 6: 3051-3064) .). In addition, NO induced immunomodulating between T-cell and endothelial cells (Bingisser RM, Holt PG Immunomodulating mechanism in the lower respiratory tract; nitric oxide mediated intraction between alveolar macrophage, epithelial cell, and T-cell, SWISS Medical Weekly. 2001; 131; 171-179.) It is known that there is a potent platelet aggregation inhibitory effect.

In this regard, the inventors of the present invention have shown that the fruiting extracts promote the proliferation of splenocytes and macrophages, induce the production of NO, and exhibit an excellent effect on the enhancement of immune sensitization in mice. By confirming that the present invention was completed.

An object of the present invention is a composition for enhancing immune function containing low fruit fruit extract as an active ingredient, health foods for enhancing immune function containing low fruit fruit extract as an active ingredient and prevention of diseases caused by lowering immune function using the same as an active ingredient And to provide a therapeutic agent.

In order to achieve the above object, the present invention provides a composition for enhancing immune function containing a low fruit fruit extract as an active ingredient.

In addition, the present invention provides a health food for enhancing immune function containing low fruit fruit extract as an active ingredient.

The present invention also provides a prophylactic and therapeutic agent for diseases caused by a decrease in immune function containing low fruit fruit extract as an active ingredient.

In addition, the present invention provides a method for the prevention and treatment of diseases caused by impaired immune function, comprising administering to a subject a pharmaceutically effective amount of low fruit fruit extract.

The low fruit extract of the present invention is effective in enhancing immune function because it increases the growth of splenocytes and macrophages in mice, increases the production of NO, and enhances immune sensitization in mice. It is useful to use as a composition for strengthening, health food for enhancing immune function, and prevention and treatment of diseases caused by lowering immune function.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for enhancing immune function, which comprises a low fruit extract extracted with water, a lower alcohol or a mixture thereof as an active ingredient.

It is preferable that the lower alcohol is a C ₁ to C ₄ lower alcohol. It is preferable to use about 5 to 20 times, preferably about 10 times, water, a lower alcohol, or a mixture thereof as the extraction solvent in the low chamber, and the lower alcohol is preferably methanol or ethanol. The mixture of water and lower alcohol is preferably, but not limited to, 10 to 90% methanol or ethanol mixture. The extraction is also performed at room temperature for about 1 to 24 hours, preferably 4 to 15 hours, most preferably 1 to 2 hours. The extraction method is preferably any one selected from the group consisting of hot water extraction, cold needle extraction, reflux cooling extraction, ultrasonic extraction and steam extraction, the low-chambered fruit extract of the present invention can be obtained by performing lyophilization after extraction.

The present inventors investigated the effect of the low fruit extract extracted as described above on the growth of spleen cells, macrophages of mice. As a result, it induces the proliferation of splenocytes (see Table 2), showed an excellent proliferation effect even in macrophages, it was confirmed to be superior to the positive control (see FIGS. 1 to 3).

The inventors then investigated the effect of low fruit extract on NO production. As a result, it was confirmed that the production of NO also effective in boosting immunity (see FIG. 4). In addition, it was also found that the effect of enhancing immune sensitization in mice was also excellent (see FIG. 5).

Therefore, the low fruit extract of the present invention can be efficiently used in the composition for enhancing immune function.

The composition for enhancing immune function of the present invention may be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, oral formulations, external preparations, suppositories, and sterile injectable solutions. Examples of carriers, excipients and diluents that can be included in the composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, Cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose ( It is prepared by mixing sucrose or lactose and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. .

Formulations for parenteral administration include sterile aqueous solutions, solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, suppositories, injections. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

In addition, the present invention provides a health food for enhancing immune function containing low fruit fruit extract as an active ingredient.

Examples of foods to which low fruit extract may be added include various foods, dairy products, beverages, gums, teas, vitamin mono- and dietary supplements, and may be used in the form of powders, granules, tablets, capsules, or beverages. Can be.

It may also be added to foods or beverages for the purpose of enhancing immune function. At this time, the amount of the extract in the food or beverage can be added in 0.01 to 15% by weight of the total food weight, the health beverage composition is added in a ratio of 0.02 to 5 g, preferably 0.3 to 1 g based on 100 ml Can be.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for containing the low fruit fruit extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. have. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the low fruit fruit extract of the present invention is a variety of nutrients, vitamins, minerals (electrolytes), synthetic flavors and flavors such as natural flavors, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid And salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the low fruit extract of the present invention may contain the flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not so critical but is usually selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the low fruiting body of the present invention.

The present invention also provides a prophylactic and therapeutic agent for diseases caused by a decrease in immune function, containing low fruit fruit extract as an active ingredient.

The disease caused by the reduced immune function is preferably any one selected from the group consisting of infectious diseases such as colds and inflammatory diseases, allergic diseases such as atopy, chronic fatigue, and cancer, but is not limited thereto. All diseases resulting from a known decrease in immune function are included in the present invention.

The preferred dosage of the low fruiting extract of the present invention depends on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the desired effect, the low fruit fruit extract of the present invention is preferably administered at 0.0001 to 500 mg / kg, preferably 0.001 to 500 mg / kg per day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

In addition, the present invention provides a method for the prevention and treatment of diseases caused by impaired immune function, comprising administering to a subject a pharmaceutically effective amount of low fruit fruit extract.

In this method, the low fruit extract of the present invention can be administered by various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intracerebroventricular injections.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples and Formulation Examples.

However, the following Examples, Experimental Examples, and Formulation Examples specifically illustrate the present invention, and the content of the present invention is not limited to Examples, Experimental Examples, and Formulation Examples.

Example 1 Low Silencer ( Broussonetiae Fructus A) extract manufacturer

<1-1> Preparation of Low Silja Extract

Low fruit (Takberry, Chinese) was purchased from medicinal herb (Baekje-Dang, Daejeon) and used. An extraction solvent corresponding to 10 times (w / v) of the medicinal herb was used, and three extraction solutions were obtained by using an ultrasonic extraction method and a reflux extraction method using hot water extraction method, 70% ethanol as a solvent as an extraction method. Each extract was concentrated under reduced pressure and lyophilized to prepare an extract. The yields different for each extraction method are shown in Table 1.

Yield of Extracts from Tree Fruit Extraction Method sign Extraction solvent Way Yield (%) W Deionized distilled water Hot water extraction, heating time 90 minutes 4.09 ES 70% ethanol Ultrasonic Extraction, Ultrasonication 90 minutes 2.80 ER 70% ethanol 90 minutes of reflux extraction, heating and reflux 2.80

Experimental Example 1 Investigation of Cell Proliferation Effect of Mouse Spleen Cells

Seven-week-old male C57BL / 6 mice (Korean Biolink, Chungbuk Negative) were extracted after the cervical spine bone. The extracted spleens were washed with HBSS (Hank's Balanced Salts Modified.Gbico BRL.), Centrifuged and separated to remove red blood cells with RBC lysing buffer (Sigma), followed by 10% FBS (fetal bovine serum, Gibco BRL. ) And penicillin (penicillin, Gibco BRL.) And streptomycin (streptomycin, Gibco BRL.) And suspended in RPMI 1640 (Hyclone) medium containing and cultured at 5% CO ₂ , 37 ℃. 96-well to search the effect of low fruiting hot water extract (W), low fruiting 70% ethanol ultrasonic extract (ES) and low fruiting 70% ethanol reflux extract (ER) prepared in Example 1 on the spleen cell proliferation Splenocytes isolated and cultured in a well plate are divided into 5 × 10 ⁵ cells / well, and the low-fat fruit extract (Hydrogen extract (W), 70% ethanol ultrasonic extract (ES) and 70% ethanol reflux extract (ER)) Was dissolved in PBS at a concentration of 2 × 10 ⁻³ to 1 × 10 ⁰ mg / ml and added. As a comparison material for the cell proliferation effect, ConA (Concanavalin A, Sigma Co., USA) and LPS (lipopolysaccharide, Sigma Co., USA) were used as 2 μg / ml, respectively. After 48 hours of incubation, add tetrazolium salt CCK-8 kit (Cell Counting Kit-8, Dojindo Laboratories, Tokyo, Japan) solution and incubate for 4 hours more, then use ELISA reader (Ceres UV 900C, Bio-tech instrument, USA). Absorbance at 450 nm was measured. The ratio of the absorbance of drug addition wells to the absorbance of control wells was calculated in% and the average value of several wells was used. The calculation is as follows.

As a result, when the low-silja extract was treated with 2.5 mg / ml at the concentration of dry medicinal herbs in the emergency cells isolated from the mice, the proliferative effect of splenocytes was 1.2-fold and 3.0-fold in the low-silja extract W, ES, and ER, respectively. , 2.0 times. This resulted in an increase of 150 ± 2.70 and 198.22 ± 0.93% of the control group when 2 μg / ml of LPS and ConA were used as the positive control group. It can be seen (Table 2).

Splenocyte Proliferation Effect of Low Silja Extract Treatment in Mouse Spleen Cells Ctrl (%) W ES ER 100 ± 0.95 124.74 ± 4.34 305.20 ± 22.85 195.96 ± 12.87

Experimental Example 2 Investigation of Cell Proliferation and NO (nitric Oxide) Formation in Mouse Macrophage Cell Line

<2-1> Investigation of Cell Proliferation in Mouse Macrophage Cell Line

Raw 264.7 cell line, a Murin macrophage cell line, was dispensed in 96 × well plates at 5 × 10 ³ cells / well and the fruiting extracts (hot water extract (W), 70% ethanol ultrasound extract (ES) and 70%) Ethanol reflux extract (ER)) was treated at a concentration of 1 × 10 ⁻³ to 1 × 10 ⁰ mg / ml. As a comparison material, ConA (Sigma Co., USA) and LPS (Sigma Co., USA) were used at 2 μg / ml, respectively. After 48 hours of incubation, add tetrazolium salt CCK-8 kit (Cell Counting Kit-8, Dojindo Laboratories, Tokyo, Japan) solution and incubate for 4 hours more, then use ELISA reader (Ceres UV 900C, Bio-tech instrument, USA). Absorbance at 450 nm was measured. The ratio of the absorbance of drug addition wells to the absorbance of control wells was calculated in% and the average value of several wells was used. The calculation is as follows.

As a result, low fruiting hot water extract (W) and 70% ethanol extract (ES and ER) all showed 185%, 265%, 276% or more cell proliferation effect at 50 μg / ml concentration as a dry medicinal equivalent. Low ethanol extracts (ES and ER) induced cell proliferation in a concentration-dependent manner, showing an excellent cell proliferation effect from 500 μg / ml to 330% or more of the control group (FIGS. 1 to 3).

<2-2> NO Production Effect of Mouse Macrophage Cell Line

Raw 264.7 cells were aliquoted into 2.5 × 10 ⁵ cells / well in 48-well plates, incubated in CO ₂ incubator for 24 hours, and treated with LPS by concentration as a positive control. The drug treatment group was treated with low fruit fruit extracts (hot water extract (W), 70% ethanol ultrasonic extract (ES) and 70% ethanol reflux extract (ER)) at 0.2, 2, 20, 200, 2000 μg / ml and 18 hours. After incubation, the supernatant was separated and NO production was measured. NO production was reacted using Griess reagent (Promega, USA) and the absorbance was measured at 535 nm.

As a result of processing the low silja extracts in dry 264.7 cells with the equivalent amount of dry medicinal herbs and collecting supernatant, the amount of NO produced was found to be dependent on the concentration. Extract (W) showed an excellent effect of about 200 ng / ml LPS used as a positive control at a concentration of 2500 μg / ml or more (Fig. 4). In addition, since the cytotoxicity was not shown at the same concentration it can be seen that the safe concentration.

Example 3 In Vitro Immune Enhancement of Low Fruit Fruit Extracts

<3-1> Immunosensitization and Drug Administration

Ovalbumin (Imject Ovalbumin, Thermo Scientific) + Aluminum (Aluminum hydroxide Gel, Sigma-Aldrich) (100 μg + 200 μg / 100 μl) was intraperitoneally administered to a 6-week-old C57BL / 6 mouse. Sensitized (day 0). After 10 days of administration, ovalbumin + aluminum was boosted by dressing (day 10). The low fruit fruit extract prepared in the above example was administered at 5 g / kg / day in dry medicine equivalents from day 0 to day 20 to compare the efficacy according to the extraction method. The control group, OVA group and OVA + Al group were orally administered the corresponding amount of distilled water. 20 days after the start of animal experiments, the abdomen was incision under ether anesthesia, blood was collected from posterior vena cava, and the spleen was extracted and used for subsequent experiments.

<3-2> Proliferation of Splenocytes for Search for Mitogen Effect

After 20 days of low-silica extract administration, the spleens extracted from each group were washed with HBSS (Hank's Balanced Salts Modified.Gbico BRL.), Centrifuged and separated to remove red blood cells with RBC lysing buffer (Sigma). % FBS (fetal bovine serum, Gibco BRL.) with penicillin, was suspended in RPMI 1640 (Hyclone) medium containing streptomycin and cultured at 5% CO _2, 37 ℃. To detect mitogen effects, splenocytes isolated and cultured in 96-well plates were dispensed at 5 × 10 ⁵ cells / well, PBS in control wells, LPS (10 μg / ml) in other wells, ConA (4 μg / ml), OVA (10 μg / ml) were treated. After culturing for 2 days, the cell proliferation was measured by CCK-8 kit, which is tetrazolium salt.

C57 BL / 6 mice sensitized OVA + Aluminium (100 μg + 200 μg / 100 μl) twice at 10-day intervals were orally injected with low-chambered water extract at a dose of 5 g / kg / day as a dry medicinal equivalent for 3 weeks. After separating the spleen and confirming the cleavage-inducing effect, it was shown that the cleavage-inducing effect of the low-chambered group (W) than the control OVA (Fig. 5).

Through the above results, the low-heated water extract of the present invention, 70% ethanol extract are all excellent effects on the cell proliferation of mouse splenocytes, the proliferation of macrophages, the promotion of NO production of macrophages, the enhancement of immune sensitization in mice The present invention suggests that low loss may be used for immune activation and immune function enhancement.

Examples of formulations for the composition of the present invention are illustrated below.

Preparation Example 1 Preparation of Pharmaceutical Formulation

<1-1> Preparation of Powder

2 g of low fruit water extract of the present invention

1 g lactose

After mixing the above components, the airtight cloth was filled to prepare a powder.

<1-2> Preparation of Tablet

100 mg of low fruit fruit 70% ethanol extract (ES) according to the present invention

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

<1-3> Preparation of Capsule

100 mg of low loss fruit 70% ethanol extract (ER) of the present invention

Corn starch 100 mg

Lactose 100 mg

2 mg magnesium stearate

After mixing the above components, the capsule was prepared by filling in gelatin capsules according to the conventional method for producing a capsule.

<1-4> Preparation of Injection Solution

10 μg / ml low fruit water extract according to the present invention

Dilute hydrochloric acid BP until pH 3.5

Injectable sodium chloride BP up to 1 ml

Dissolve the wood mushroom mycelium powder according to the present invention in an appropriate volume of sodium chloride BP for injection, adjust the pH of the resulting solution to pH 3.5 with dilute hydrochloric acid BP, and adjust the volume with sodium chloride BP for injection and sufficiently Mixed. The solution was filled into a 5 ml Type I ampoule made of clear glass, encapsulated under an upper grid of air by dissolving the glass, and sterilized by autoclaving at 120 ° C. for at least 15 minutes to prepare an injection solution.

Preparation Example 2 Preparation of Food

Food containing the low fruit fruit extract of the present invention according to the present invention was prepared as follows.

<2-1> Preparation of Flour Food

0.1 to 10.0 parts by weight of the low fruit water extract of the present invention was added to the flour, and using the mixture, bread, cake, cookies, crackers and noodles were prepared in a conventional manner to prepare foods for health promotion.

<2-2> Preparation of Soups and Gravys

0.1-1.0 parts by weight of low fruit water extract of the present invention was added to soups and gravy to prepare meat products for health promotion, soups and gravy of noodles in a conventional manner.

<2-3> Preparation of Ground Beef

10 parts by weight of low fruit water extract of the present invention was added to the ground beef to prepare a ground beef for health promotion in a conventional manner.

<2-4> Production of Dairy Products

0.1 to 1.0 parts by weight of the low fruit water extract of the present invention was added to milk, and various dairy products such as butter and ice cream were prepared in a conventional manner using the milk.

<2-5> Preparation of Wire

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.

The low fruit water extract of the present invention was decompressed and concentrated in a vacuum concentrator, and the dried product obtained by drying with a spray and a hot air dryer was pulverized with a particle size of 60 mesh to obtain a dry powder.

The grains, seeds and the mycelium mycelium mycelium powder according to the present invention were prepared in the following ratio to prepare a conventional method.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

Low fruit water extract of the present invention (1 part by weight),

(0.5 part by weight),

Foxglove (0.5 part by weight)

Preparation Example 3 Preparation of Beverage

The beverage containing the low fruit water extract of the present invention was prepared as follows.

<3-1> Preparation of Health Beverage

Instant sterilization by homogeneously mixing sub-components such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and low fruit water extract according to the present invention. Then, it was packaged in a small packaging container such as glass bottles, plastic bottles to prepare a healthy beverage.

<3-2> Preparation of Vegetable Juice

0.5 g of the low fruit water extract of the present invention was added to 1,000 ml of a vegetable juice such as tomato or carrot to prepare a vegetable juice for health promotion in a conventional manner.

<3-3> Preparation of Fruit Juice

0.1 g of low fruit water extract of the present invention was added to 1,000 ml of fruit such as apples or grapes to prepare fruit juice for health promotion in a conventional manner.

Since the low fruit fruit extract of the present invention is effective in strengthening the immune function, allergic diseases such as infectious diseases such as a cold caused by a composition for enhancing immune function, health food for enhancing immune function, and lowering immune function, and inflammatory diseases such as atopic dermatitis, etc. It can be usefully used for the development of preventive and therapeutic agents such as chronic fatigue and cancer.

1 is a graph showing the proliferation effect of macrophages according to the treatment of low fruiting hot water extract (W) of the present invention in mouse macrophages.

Figure 2 is a graph showing the proliferation effect of macrophages according to the low fruiting 70% ethanol ultrasonic extract (ES) treatment of the present invention in mouse macrophages.

Figure 3 is a graph showing the proliferation effect of macrophages according to the low fruiting 70% ethanol reflux extract (ER) treatment of the present invention in mouse macrophages.

Figure 4 is a graph showing the effect of inducing NO production according to the low fruit fruit extract treatment of the present invention in mouse macrophage line.

5 is a graph showing the mitogen effect of the low fruit fruit hot water extract in OVA + Al sensitized mice.

Claims (12)

delete delete delete delete A health food composition for enhancing immune function, which contains as an active ingredient an extract of Broussonetiae Fructus extracted with ethanol or an ethanol aqueous solution as a solvent. 6. The health food composition for enhancing immune function according to claim 5, wherein the extract is extracted by ultrasonic extraction or reflux extraction. delete delete delete delete delete delete

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