KR20100041221A - Manufacturing method of black ginseng comprising step irradiating ultraviolet rays - Google Patents

Abstract

PURPOSE: A manufacturing method of black ginseng is provided to increase the content of ginsenoside and crude saponin by irradiating ultraviolet rays on steamed ginseng after repeating a steaming and a drying process of the ginseng. CONSTITUTION: A manufacturing method of black ginseng comprising a ultraviolet irradiation step includes a step for steaming ginseng; repeating a drying process of the steamed ginseng; and irradiating ultraviolet rays for 2 ~ 60 hours on the ginseng which is steamed at least one or more times; and repeating a steaming and a drying processes 9 times.

Description

Manufacturing method of black ginseng comprising ultraviolet irradiation step {Manufacturing method of black ginseng comprising step irradiating ultraviolet rays}

The present invention relates to a method for producing black ginseng, and more particularly, to a method for preparing black ginseng further comprising an ultraviolet irradiation step for increasing the content of irradiated ponin and ginsenoside in the method of repeatedly manufacturing black ginseng. .

Ginseng is a herbal medicine that is well known for its efficacy and efficacy, and has been used for thousands of years. Such ginseng is often used as is, or dried ginseng, steamed ginseng also used to produce red ginseng.

When red ginseng is produced by steaming ginseng, it has been reported that the pharmacological action of ginseng is changed to produce ingredients that are not present in ginseng, or increase the content of existing ingredients.

To capture the $ 3.5 billion global ginseng market, Korean bio venture companies are working hard to develop new ginseng-related materials worth $ 3.5 billion annually. Until now, the only company that has standardized ginseng as a functional food and is making a profit in the global market is “Pharmaton,” a subsidiary of Boehringer Ingelheim, Switzerland. In Korea, national research on ginseng and commercialization of venture industry are in progress.

Korean red ginseng has a historical record of about 1,000 years ago (there is a record of red ginseng in Goryeo-do, written by the Chinese Song Dynasty). have. In general, the numerical objectives of herbal medicines are known as: 1) reducing side effects such as toxicity, 2) altering herbal properties and enhancing drug efficacy, 3) improving storage and storage, and 4) correcting taste and odor and color. In general, red ginseng undergoes a secondary component conversion during an appropriate heat treatment process, thereby generating new active ingredients unique to red ginseng or red ginseng, and some active ingredients increase in content. As such, new ginsengs that do not exist in ginseng are produced according to the processing method of ginseng, and new processed ginsengs can be developed that have better effects on various physiological activities including anticancer activity than ginseng (Okamura N. et al., Biol.Parm. Bull., 17 (2), p. 270, 1994).

In China, it develops a method of mass-producing Rg3, a ginseng saponin, which is rarely present in white ginseng and is present in red ginseng, and is used for anticancer treatment under the trade name 'Shenyi'. In Korea, the representative product of functional ginseng was 'Seonsam' developed by bioventure company Ginseng Science in 1998. It was developed as Sesamsamjeong in 2001 and has been launched in the domestic health functional food market (Keum YS et al., Antioxidant). and anti-tumor promoting activities of the methanol extract of heat-processed ginseng, Cancer Lett.,, 150 (1), pp. 41-48, 2000).

In addition, 'Hwang Sam EX' co-developed by Korea Research Institute of Biotechnology and Bio-Venture Company, Vitigin, Nam-Sil Farm Ginseng Farming Co., Ltd. Unigen is currently developing several types of processed ginseng, such as Biomax, which was developed jointly with UPI in the US, Six Plus of CJ, and Yellow Ginseng of Ildong Pharm.

As mentioned above, a lot of research has been conducted using ginseng, and as another method, there is a great interest in manufacturing black ginseng having more active ingredients than ginseng through the process of steaming and drying ginseng several times more than red ginseng. .

However, the manufacturing method of the black ginseng known to date is complicated in the manufacturing process, it takes a long time, and in many cases fail in the manufacturing process is industrialized at a high price, it is difficult to use in daily life. Therefore, the following studies have been conducted to solve these problems.

Korean Registered Patent Publication No. 10-0543862 (black ginseng and black ginseng concentrate with excellent content of active ingredient) is a black ginseng prepared by steaming black ginseng nine times and drying them in an ocher drying room ten times, and compared to specific ginsenoside components. It is disclosed that black ginseng and black ginseng concentrate with increased content of.

In addition, Korean Patent Publication No. 10-0529475 (Method of manufacturing black ginseng and black ginseng) is a method for producing black ginseng and black ginseng with increased saponin components by washing and drying the ginseng and then steaming and drying nine times. Is open to the public.

In addition, Korean Patent Publication No. 10-0496418 (the method of manufacturing black rice ginseng and the black rice ginseng prepared using the same) includes preparing black rice ginseng by using a 10-pack 10 method of the roots of ginseng containing most saponins. A method is disclosed.

Black ginseng manufacturing methods as described above are those that increase the content of irradiated saponins, specific ginsenosides by varying the conditions such as steaming temperature and time. Nevertheless, there is a problem that the content of the irradiated saponins, ginsenosides is not sufficient.

The problem to be solved by the present invention is to provide a method for producing black ginseng that can further improve the content of the irradiated saponins, ginsenosides.

The present invention to achieve the above object is

In the method for producing black ginseng repeating the steps of steaming ginseng and drying steamed ginseng,

At least one time after steaming, the method provides a method for producing black ginseng, further comprising irradiating ultraviolet rays for 2 to 60 hours.

The method for preparing black ginseng according to the present invention has an advantage of increasing the content of irradiated saponin and ginsenosides compared to the conventional method for preparing black ginseng.

It will be described in detail for the practice of the present invention as described above.

Black ginseng manufacturing method according to the present invention is known in the conventional manufacturing method (for example, Korean Patent Publication No. 10-0543862, Korean Patent Publication No. 10-0529475, Korean Registered Patent described in the background art of the invention) The black ginseng manufacturing method and background art described in Korean Patent Publication No. 10-0496418 are not described, but Korean Patent Publication No. 10-0617330, Korean Patent Publication No. 10-0729214, and Korean Patent Publication No. 10-0754253 Irradiating UV light for 2 to 60 hours after steaming steamed ginseng in order to increase the content of irradiated phonynin and ginsenoside while using the same method of manufacturing black ginseng described in Korean Patent Publication No. 10-0844990) It characterized in that it further comprises.

In the present invention described above, the term "at least once" means that the number of steaming steps may be higher than that of the steaming company, but the UV irradiation step may be performed only once, but the content of irradiated ponin and ginsenoside may be increased. Is that you can achieve

In addition, the ultraviolet light used in the present invention is an area invisible to the human eye in the electromagnetic spectrum, which is invisible to the human eye, and can use any of 320-400 nm UV-A, 280-320 nm UV-B, and 100-280 nm UV-C. Do.

In addition, the above-mentioned ultraviolet irradiation time is measured by various experimental results of the present inventors, and at least two hours should be irradiated for increasing the content of the irradiated phononine and ginsenoside, but the width of the increased content increases with the irradiation time. Over time, the meaning of increase is insignificant, and the entire manufacturing process is too long.

Hereinafter, the present invention will be described through specific examples and test examples. It is apparent that the present invention is not limited by the examples and test examples described below.

<Preparation of Black Ginseng According to Conventional Methods>

After purchasing 5-year-old ginseng produced and distributed in Geumsan, it was lightly washed with water so that only the soil was removed and put into a ginseng steamer. Subsequently, the first steamed hemp was first dried for about 12 hours in a drying chamber maintained at 60 ° C.

As described above, steaming and drying were repeated eight more times, black ginseng was prepared through a total of nine steaming and drying processes.

<Production of Black Ginseng Including Ultraviolet Irradiation Step>

The same process as the manufacturing method of the conventional black ginseng as described above, but there is a difference in the ultraviolet irradiation step as shown in Table 1 between the steaming and drying process.

The apparatus for irradiating ultraviolet rays used an ultraviolet irradiator for disinfecting dishes used in a general restaurant. The ultraviolet ray irradiated at this time is an ultraviolet ray having a wavelength of approximately 250 nm.

number One ultraviolet irradiation time UV irradiation frequency Remarks Example 1 1 hour 30 minutes One After primary steaming and before drying Example 2 2 One After primary steaming and before drying Example 3 15 One After primary steaming and before drying Example 4 30 One After primary steaming and before drying Example 5 60 One After primary steaming and before drying Example 6 70 One After primary steaming and before drying Example 7 15 3 1st, 2nd, 3rd steam before drying Example 8 15 3 1st, 3rd, 6th steam before drying Example 9 15 5 1st, 3rd, 5th, 7th, 9th steam before drying Example 10 15 9 After steaming from 1st to 9th, before drying

<Preparation of Black Ginseng Extract>

20 g of black ginseng was taken from the prepared black ginseng, pulverized, 100 ml of water was added thereto, and the mixture was left at room temperature for 5 hours, followed by 1 liter of methanol, followed by 3 hours of reflux extraction.

The extracted solution was cooled to room temperature, filtered, and methanol was removed using a vacuum condenser to obtain black ginseng extract. 200 ml of water was added to the black ginseng extract to suspend and 200 ml of ether was added to remove the nonpolar material.

200 ml of saturated butanol was added to the remaining aqueous layer, and the active ingredient was extracted. Butanol was removed by a vacuum condenser to obtain a final butanol extract.

This extract was used for the test described later.

<Test Example; Saponin Component Analysis>

In order to analyze the saponin component of the black ginseng extract obtained above, the extract was dissolved in methanol and analyzed by HPLC on the filtrate filtered with a filter.

Probeponin (mg / g) Rb1 (mg / g) Rb2 (mg / g) Rc (mg / g) Re (mg / g) Rg1 (mg / g) Rg3 (mg / g) Rh1 (mg / g) Conventional 38.70 0.17 0.09 0.65 0.96 0.48 0.55 0.19 Example 1 39.20 0.21 0.11 0.72 0.96 0.48 0.57 0.20 Example 2 70.25 0.39 0.42 1.28 2.01 1.99 2.85 1.97 Example 3 105.22 1.09 1.11 2.15 2.81 2.55 3.99 2.01 Example 4 112.3 1.74 1.25 2.85 3.07 2.95 4.30 2.78 Example 5 120.3 2.81 2.72 3.94 4.78 4.55 6.73 3.89 Example 6 120.5 2.81 2.70 3.95 4.88 4.55 6.72 3.89 Example 7 140.3 4.21 4.12 5.07 6.01 6.87 7.02 5.12 Example 8 142.3 4.24 4.52 5.18 6.01 6.52 7.02 5.27 Example 9 162.7 5.89 6.01 7.02 6.75 8.21 8.41 7.04 Example 10 198.7 6.91 7.28 8.01 7.27 9.78 9.92 9.52

As shown in Table 2, Example 1 irradiated with ultraviolet rays once for 1 hour 30 minutes showed a slightly increased aspect than the conventional one, but the level was insufficient, but Example 2 irradiated for 2 hours and 15 hours Example 3. In Example 4 irradiated for 30 hours and Example 5 irradiated for 60 hours, a significant amount of saponin increase was confirmed, and in Example 6 irradiated for 70 hours, the difference from Example 5 was different. I could not see it.

In addition, it was confirmed that the saponin content increased in Examples 7, 8, 9, and 10, in which one irradiation time was 15 hours and the number of irradiation was increased.

Claims (1)

In the method for producing black ginseng repeating the steps of steaming ginseng and drying steamed ginseng, At least once after steaming black ginseng manufacturing method characterized in that it further comprises the step of irradiating ultraviolet rays for 2 to 60 hours.