KR20090118330A - 마우스 신경아세포종 세포의 내인성 프리온 단백질의발현을 억제하는 siRNA 및 이를 이용한 소해면상뇌증특이적인 세포 모델 - Google Patents
마우스 신경아세포종 세포의 내인성 프리온 단백질의발현을 억제하는 siRNA 및 이를 이용한 소해면상뇌증특이적인 세포 모델 Download PDFInfo
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- KR20090118330A KR20090118330A KR1020080044057A KR20080044057A KR20090118330A KR 20090118330 A KR20090118330 A KR 20090118330A KR 1020080044057 A KR1020080044057 A KR 1020080044057A KR 20080044057 A KR20080044057 A KR 20080044057A KR 20090118330 A KR20090118330 A KR 20090118330A
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Abstract
Description
siRNA | 시작 | 뉴클레오타이드 염기서열 ( 5′- 3′) | 끝 | 사이즈 |
서열번호1 | 182 | GGCCCTCTTTGTGACTATGTGGACT | 206 | 25mer |
서열번호2 | 819 | CAGGCCTATTACGACGGGAGAAGAT | 843 | 25mer |
Claims (10)
- 서열 번호 1로 표시되는 siRNA(small interfering RNA).
- 제 1 항에 있어서, 상기 siRNA는 마우스 프리온 유전자(서열 번호 3, Genebank Accession No.NM_011170)의 발현을 억제하는 것임을 특징으로 하는 siRNA.
- 서열 번호 2로 표시되는 siRNA(small interfering RNA).
- 제 3 항에 있어서, 상기 siRNA는 마우스 프리온 유전자(서열 번호 3, Genebank Accession No.NM_011170)의 발현을 억제하는 것임을 특징으로 하는 siRNA.
- 소 프리온 유전자(boPrnp ORF, 서열 번호4)를 진핵세포 발현 벡터에 클로닝하는 제1단계;상기 벡터를 형질감염 운반체를 통하여 마우스 신경 세포주 내로 이식하는 제2단계; 및상기 마우스 신경 세포주에 제1항 또는 제2항의 siRNA을 도입하여 마우스 프리온 단백질의 발현을 억제하는 제3단계;를 포함하는 것을 특징으로 하는 소해면상 뇌증(Bovine Spongiform Encephalopathy)에 특이적인 세포 모델 제조 방법.
- 제 5 항에 있어서, 상기 마우스 신경 세포주는 N2a (mouse neuroblastoma)인 것을 특징으로 하는 방법.
- 제 5 항에 있어서, 상기 마우스 신경 세포주는 50 passage 이하의 세포임을 특징으로 하는 방법.
- 제 5 항에 있어서, 상기 siRNA의 농도는 20~400nM 인 것을 특징으로 하는 방법.
- 제 5 항에 있어서, 상기 siRNA의 도입은 트랜스펙션 (transfection)에 의함을 특징으로 하는 방법.
- 제 5 항 내지 제 8 항 중 어느 한 항의 방법에 의하여 제조된 소해면상뇌증에 특이적인 세포모델.
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KR1020080044057A KR101043252B1 (ko) | 2008-05-13 | 2008-05-13 | 마우스 신경아세포종 세포의 내인성 프리온 단백질의발현을 억제하는 siRNA 및 이를 이용한 소해면상뇌증특이적인 세포 모델 |
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KR1020080044057A KR101043252B1 (ko) | 2008-05-13 | 2008-05-13 | 마우스 신경아세포종 세포의 내인성 프리온 단백질의발현을 억제하는 siRNA 및 이를 이용한 소해면상뇌증특이적인 세포 모델 |
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KR1020110022530A Division KR101048415B1 (ko) | 2011-03-14 | 2011-03-14 | 마우스 신경아세포종 세포의 내인성 프리온 단백질의 발현을 억제하는 siRNA 및 이를 이용한 소해면상뇌증 특이적인 세포 모델 |
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KR20090118330A true KR20090118330A (ko) | 2009-11-18 |
KR101043252B1 KR101043252B1 (ko) | 2011-06-21 |
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