KR20090099939A - Method for manufacturing soysource or soybean paste used chitooligosaccharides - Google Patents

Abstract

PURPOSE: A method for preparing a soy sauce or soybean paste using chitooligosaccharides is provided to use a chitooligosaccharide solution with molecular weight of 13,000 or less as water. CONSTITUTION: A method for preparing a soy sauce or soybean paste using chitooligosaccharides comprises a step of using a chitooligosaccharide solution as water in a producing process of soy sauces or soybean paste. The average molecular weight of the chitooligosaccharide is 13,000 or less. A method for preparing the chitooligosaccharide solution comprises the following steps of: dissolving chitosan in hydrochloric acid to obtain a chitosan-hydrochloride solution; adding a chitosanase to the chitosan-hydrochloride solution to form a chitosanoligosaccharide; filtering the chitosanoligosaccharide-containing solution to remove unreacted materials and refining with a ultrafiltration membrane; and adding caustic soda to the refined solution to neutralize it and desalting and concentrating the neutralized solution.

Description

Method for manufacturing soy sauce or miso using chitooligosaccharide with high body absorption {Method for manufacturing soysource or soybean paste used chitooligosaccharides}

The present invention relates to a method for producing soy sauce and soybean paste using a chitooligosaccharide solution. Specifically, a chito oligosaccharide having a molecular weight of 13,000 or less having high molecular weight absorption is distributed by passing a chito oligosaccharide solution decomposed chitosan using an enzyme through an ultrafiltration membrane. The present invention relates to a method for producing soy sauce and miso using chitooligosaccharide solution as water for preparing soy sauce and miso.

Chitosan is a basic polysaccharide derived from nature, chitin is the main raw material. Chitin is a natural polymer in the shell of crustaceans such as crabs and shrimps, the epidermis and mushrooms of fungi, and the fungal cell walls. It is an enormous organism that is equivalent to cellulose, which is estimated to be 2 million tons per year. Resource. Chitosan is a deacetylate of chitin and used as a flocculant or a dehydrating agent in water treatment. The application range is expanding. In other words, the development of food additives, soil improvers, plant actives, natural pesticides, cosmetic materials, clothing and textile materials, artificial skin, carriers have been tried (Kim Se-kwon, Food Industry, 1997, 140: 7-32).

In the chitosan oligosaccharide, chitin, which is mainly contained in shellfish, crab shells, and the like, is separated and purified into chitin and chitosan by crushing crude raw materials, desalting, removing proteins, and removing impurities, and separating and purifying chitin and chitosan. Was prepared by appropriately decomposing the chitin or chitosan degrading enzyme isolated from Streptomyces SP. Chitosan is also known to exhibit a variety of bioactivity as a bioactive material. Representative biological activities include anti-cancer effect, blood cholesterol lowering effect, immune enhancing effect, anti-sugar effect, wound healing effect, antibacterial effect.

In addition, there are a chemical method and a biological method as a method for producing chitosan oligosaccharides by hydrolyzing chitosan. Hydrochloric acid hydrolysis, which is a chemical decomposition method, is not suitable as a food or food ingredient manufacturing method, and the KFDA currently regulates its use. Therefore, chitosan oligosaccharides manufactured by biological methods are mostly used. It is commercially available. However, most of chitosan oligosaccharides prepared by biological methods are commercially available as salt-type chitosan oligosaccharides without removing the salts used in the decomposition process. However, because chitosan produced by this process has a molecular weight of several hundred thousand to millions of polymers. There is a disadvantage that the absorption rate in the body falls.

On the other hand, conventional soy sauce and soybean paste has a high content of chlorine ions that are known to cause high blood pressure because it is made only purely meju and brine. Chitosan is very useful for hypertension because it reacts with chlorine, absorbs and releases it, and reduces blood concentration, thereby lowering the activity of ACE, an enzyme of blood pressure control that requires chlorine ions for its activation, and thus reducing blood pressure. By reducing the blood chlorine concentration by inhibiting the excessive secretion of insulin has the effect of suppressing the increase in blood pressure due to obesity.

Therefore, the present inventors have conducted extensive research to solve the above problems, and as a result, the anti-cancer activity due to the increase in immunity and cell reactivity by using chitooligosaccharide having high bioabsorption rate prepared according to the present invention for the preparation of soy and miso The present invention was completed by confirming that it can have various physiological activities such as antibacterial activity, body cholesterol improving action and hypertension suppressing action.

To overcome the problems of the conventional chitosan, such as water-insoluble and inferior in the body absorption rate, and to provide a method for producing soy and miso using a chitooligosaccharide aqueous solution in which chitosan with high body absorption rate is dissolved in water.

The present invention relates to a method for producing soy sauce or soybean paste, characterized by using a chitooligosaccharide solution having high absorption in the body as soy sauce or soybean paste preparation water.

The present invention prepares a solution of chitooligosaccharides having a molecular weight of 13,000 or less, and uses it as water for the preparation of soy sauce, thereby increasing the absorption rate of chitooligosaccharides in the body, enhancing the immune system and anti-cancer activity, improving cholesterol in the body, and inhibiting hypertension. Soy sauce and miso can be prepared.

The present invention relates to a method for producing soy sauce or soybean paste, characterized by using a chitooligosaccharide solution having high absorption in the body as soy sauce or soybean paste preparation water. The present invention will be described in more detail as follows.

The present invention provides a solution in which chitooligosaccharides having a molecular weight of 13,000 or less having a high water absorption in soy sauce or soybean paste manufacturing water are dissolved. The molecular weight of the chitooligosaccharide is preferably 1,000 to 13,000, more preferably 3,000 to 8,000.

 If the molecular weight exceeds 13,000, the absorption rate in the body is not high, and if the molecular weight is less than 1000, there is a problem in that the production cost increases because the absorption is easy but the substrate-to-enzyme ratio must be increased and the membrane must be filtered with a molecular weight of 1,000 at the final stage. .

According to one embodiment of the invention, the step of preparing chitooligosaccharides

(1) dissolving chitosan in a solvent to prepare a chitosan solution;

(2) preparing chitooligosaccharide by adding chitosanase to chitosan solution;

(3) filtering the chitooligosaccharide-containing solution to remove unreacted material and purifying by using an ultrafiltration membrane; And

(4) neutralizing the purified solution and filtering and desalting and concentrating with a nano filtration membrane

It may include.

In the step (1), an acid such as hydrochloric acid may be used as the solvent, and chitosan may be a known conventional chitosan. Preferably, chitosan hydrochloride can be prepared by dissolving chitosan as hydrochloric acid. The viscosity of chitosan is preferably 7 to 15 cps, more preferably 10 cps, the concentration of chitosan is preferably 4 to 6% (w / v), more preferably 5%. The concentration of hydrochloric acid as a solvent is preferably 0.2 to 0.3 M, most preferably 0.23 M. However, as long as the decomposition conditions are adjusted such that the pH of the chitosan hydrochloride solution is 5.0 to 5.5, more preferably about 5.0, the present invention is not limited thereto.

In the step (2), preferred chitosan degrading enzyme Bacillus sp. Endo-type chitosanases derived from microorganisms. Chitooligosaccharides are prepared by adding chitosanase to approximately 5 units of a 5% chitosan solution, or chitosanase at about a 1: 2 ratio of substrate to enzyme ratio under the same conditions. The reaction temperature is preferably 40 ° C. and the reaction time is about 36 hours.

In the step (3), the filter at the time of filtration may be used a prefilter, Zeta filter and the like. The pore size of the filter is most preferably 1 μm for the prefilter and 0.7 μm for the Zeta filter. Filtering step by step to remove the unreacted material and purified chitooligoic acid solution using an ultrafiltration membrane (ultrafiltration membrane). The size of the membrane is determined by the molecular weight limit (MWCO; Molecular weight cut-off), which is the size of the molecular weight passing through the pores of the membrane. Preferably, it is preferable to use an ultrafiltration membrane having an MWCO of about 30,000. The purification step using the ultrafiltration membrane may be performed once or several times as necessary. By repeatedly adjusting the membrane size of the ultrafiltration membrane in the process of purification can be obtained a chitooligosaccharide at a desired molecular weight.

In step (4), it is possible to use a basic solution for the neutralization of the solution, and most preferably a caustic soda solution. The neutralized solution is concentrated with a nanofiltration membrane and desalted with repeated addition of purified water. MWCO of the nanofiltration membrane is most preferably about 300. It is preferable to repeat desalination using a nanofiltration membrane as needed. The desalted solution can be concentrated to 20-25 BriX, sterilized by high temperature and short time sterilization, and dried to produce high purity chitooligosaccharides.

Since the molecular weight is 13,000 or less by the above method, a high molecular weight water-soluble chitosan in the body can be produced. These chitooligosaccharides improve the absorption rate in the body to enhance the efficacy of chitosan, as well as inhibit the absorption of fatty acids in the body to regulate the amount of triglycerides, and lowers blood cholesterol levels. In addition, it has been found that it has an excellent effect on the enhancement of immunity to increase the body's immunity by activating the immune system and to inhibit the proliferation of microorganisms by inhibiting the proliferation of microorganisms.

The present invention provides a method for producing soy sauce or miso using the chitooligosaccharide aqueous solution prepared by the above method as water for preparing soy sauce or miso.

Since conventional chitosan is water-insoluble, it cannot be used as preparation water for preparing foods such as enteric foods, and is added as powder. Therefore, the absorption rate of chitosan in the final produced food was low. However, since the chitooligosaccharide according to the present invention can be easily dissolved in water to prepare an aqueous solution of chitooligosaccharide, the absorption rate of the chitosan contained in the final food product can be increased by using it as a preparation water during the preparation of soy or miso. .

Methods for preparing soy sauce or miso using the aqueous solution of chitooligosaccharide according to the present invention include conventional methods known to those skilled in the art.

EXAMPLE

The following examples are intended to specifically describe the contents of the present invention, whereby the scope of the present invention is never limited and can be interpreted.

Example  One

150 kg of chitosan (Tahoon Bio Co., Ltd.) with purified water 2,800L and viscosity of 10cps was added to the enzyme reactor equipped with a stirrer, followed by swelling at 150 rpm for 2 hours while maintaining the reactor temperature at 40 ° C. To dissolve chitosan. Check that the pH of the chitosan solution is around 5.0, and add 300,000 units (8.571g, enzyme activity; 35,000 units / g) of chitosanase (Ami-Kogen) in 500 ml of purified water After standing about, the stirring speed was lowered to 120 rpm and hydrolyzed for 36 hours. The solution was filtered in the order of 20 inch prifilter (1 μm), Zeta filter (0.7 μm) to remove unreacted material and purified by ultrafiltration membrane (MWCO 30,000, Samho S & T) at 500 L / hr flow rate. The purified dodr was condensed with a nanofiltration membrane (MWCO 300) with an outflow rate of 1.5 ton / hour until the solution reached 15BX, 1M caustic soda solution was added until pH 7.2, and then condensed with the membrane (MWCO 300). Purified water was repeatedly added until the electrical conductivity of the membrane passing liquid was 0.1 ms / cm or less, then desalted, concentrated, sterilized by high temperature and short time sterilization, and dried (Spray Dryer) to obtain high purity chitooligosaccharide at about 142 kg (95% yield). Got it.

<Molecular weight measurement>

Average Molecular Weight (Mw) of the prepared high purity chitooligosaccharide was measured by an automatic molecular weight measuring instrument (Viscotek corp. Model Y-500) based on the viscosity method principle. As a result of preparing and analyzing a solution of 1.2 g / dL to 0.04 g / dL in a 0.1 M AcOH-0.2 M NaCl mixed solvent, the average molecular weight (Mw) of the prepared chitosan oligosaccharide was 5,600.

Production Example  One

The chitooligosaccharide solution prepared according to the method of Example 1 was used as soy water to dissolve 17 to 20% by weight with sun salt, which was then removed. Soy sauce was prepared by further aging the liquid phase for 60 days or more.

Production Example  2

The chitooligosaccharide powder prepared according to the method of Example 1 was dissolved in purified water at 1 to 5% by weight in soy water, and the soy water was used to dissolve the salt in 17 to 20% by weight with natural salt. After fermentation for 25 to 25% by weight, the solids and the liquid were separated after fermentation for 45 to 60 days, and the liquid phase was further aged for 60 days to prepare soy sauce.

Production Example  3

The chitooligosaccharide solution prepared according to the method of Example 1 was used as soy water to dissolve 17 to 20% by weight with sun salt from which the water was removed, followed by 17 to 25% by weight of meju, followed by fermentation for 45 to 60 days. After separation, the solids were aged for 6 months or longer to prepare miso.

Production Example  4

The chitooligosaccharide powder prepared according to the method of Example 1 was dissolved in purified water at 1 to 5% by weight in soy water, and then 17 to 20% by weight of natural salt was removed using natural salt to remove the meju. Flour to 25 to 25% by weight, after the fermentation for 45 to 60 days to separate the solids and liquid phase was aged for more than 6 months to prepare soy sauce.

Claims (3)

A method for producing soy sauce or soybean paste, characterized by using a chitooligosaccharide solution having high absorption in the body as soy sauce or soybean paste preparation water. The method for producing soy sauce or miso according to claim 1, wherein the average molecular weight of chitooligosaccharide is 13,000 or less. The method of claim 1, wherein the chitooligosaccharide solution (1) dissolving chitosan with hydrochloric acid to prepare a chitoate solution; (2) preparing chito-oligosaccharides by adding chitosanase to chitosan solution; (3) filtering the chitooligosaccharide-containing solution with a filter to remove unreacted substances and purifying by using an ultrafiltration membrane; And (4) neutralizing by adding caustic soda solution to the purified solution, desalting and concentrating with a nanofiltration membrane Method for producing soy sauce or miso characterized in that it is manufactured with.