KR20090088856A - Enzyme systems for saccharification of plant cell wall polysaccharides - Google Patents

Abstract

The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and/or depolymerize cellulose. These systems have a number of applications. Some embodiments relate to a method of producing ethanol using the cell wall degradative systems of the present invention. ® KIPO & WIPO 2009

Description

ENZYME SYSTEMS FOR SACCHARIFICATION OF PLANT CELL WALL POLYSACCHARIDES}

[Cross Reference of Related Application]

This application is a continuing application of United States Patent Application No. 11 / 121,154, filed May 4, 2005, and claims priority of US Provisional Application No. 60 / 567,971 (filed May 4, 2004), the contents of which are incorporated herein by reference. Are incorporated herein by reference in their entirety.

[Statement of research or development supported by federal government]

The present invention relates to Contract Number SA7528051 E awarded by the National Oceanic and Atmospheric Administration (NOAA) and Contract No. DEB0109869 awarded by the National Science Foundation (NSF). Under government support. The government has some rights in the invention.

[Sequence list]

This application contains a long list of sequences submitted via three CD-Rs in place of printed paper copies and is incorporated herein by reference in its entirety. The CD-R, recorded September 14, 2005 in the associated US application Ser. No. 11 / 121,154 (filed May 4, 2005), is labeled "CRF", "Copy 1" and "Copy 2", respectively. And each contains only one identical 828 KB file (18172121.APP).

[Technical Field]

The present invention relates generally to degradative enzymes and systems. In particular, the present invention relates to plant cell wall degrading enzymes and related proteins found in Microbulbifer degradans , systems comprising such enzymes and / or proteins, and methods of using such systems to obtain ethanol. will be.

Cellulase and related enzymes have been utilized in food, beer, wine, animal feed, textile manufacturing and laundry, pulp and paper industries, and agricultural industries. Various such uses are described in M. K. Bhat, "Cellulases and related enzymes in biotechnology" (Biotechnical Advances 18 (2000) 355-383), the contents of which are incorporated herein in their entirety by reference.

The cell wall of a plant consists of a heterogeneous mixture of complex polysaccharides that interact through covalent and non-covalent means. Complex polysaccharides of higher plant cell walls include, for example, cellulose (β-1,4 glucan), which constitutes 35-50% of the carbon typically found in cell wall components. To form semi-crystalline cellulose microfibers, the cellulose polymers associate themselves through hydrogen bonding, van der Waals interactions and hydrophobic interactions. These microfibers also include amorphous regions, commonly known as amorphous cellulose. Cellulose microfibers include hemicellulose (eg, including xylan, arabinan, and mannan), pectin (eg, galacturonan and galactan), and various other β-1,3 and β-1, It is embedded in a matrix of 4 glucans. These matrix polymers are often substituted, for example, with arabinose, galactose, and / or xylose residues to obtain high complex arabinoxsilanes, arabingalactans, galactomannans, and xyloglucans. The hemicellulose matrix is then surrounded by polyphenolic lignin.

The complexity of the matrix makes it difficult to degrade by microorganisms, since the lignin and hemicellulose components must be degraded before the enzyme can work on the core cellulose microfibers. Typically, in order to release the constituent monosaccharides, different microbial communities are required to degrade cell wall polymers. For saccharification of plant cell walls, lignin must be permeated and hemicellulose must be removed to allow cellulose-degrading enzymes to act on their substrates. For industrial saccharification of the cell walls, a large amount of primary fungal cellulase was added to the processed feedstock treated with sulfuric acid diluted at high temperature and pressure to permeabilize the lignin and partially glycosylate the hemicellulose component. .

Saccharophagus degradans strain 2-40 (herein referred to as "S. degradans 2-40" or "2-40") is a marine bacterium that degrades complex polysaccharides (CP). It is a representative emerging group. S. degradans has been deposited with the American Type Culture Collection and has accession number ATCC 43961. S. degradans 2-40, already known and synonymous with Microbulbifer degradans strain 2-40 (" M. degradans 2-40"), is a Chesapeake Bay watershed Is a marine □ -proteobacterium isolated from the salt marsh cord grass, Sparina alterniflora . Consistent with its separation from the degradation of plant components, S. degradans strains 2-40 break down many complex polysaccharides, including cellulose, pectin, xylan, and gutin, which are common components of the cell walls of higher plants. can do. S. degradans strains 2-40 can also degrade protein, starch, pullulan, and alginic acid and algae cell wall components such as agar, agarose, and laminarin. In addition to breaking down this plethora of polymers, S. degradans strains 2-40 can utilize each polysaccharide as the only carbon source. Thus, S. degradans strains 2-40 are not only excellent models of microbial degradation of insoluble complex polysaccharides (ICPs), but can also be used as a paradigm for the complete metabolism of these ICPs. ICPs are polymerized saccharides used for structure and morphology in animals and plants. They are insoluble in water and therefore difficult to cause chemical changes.

Microbulbifer degradans strains 2-40 require at least 1% sea salt for growth and will tolerate salt concentrations as high as 10%. It is a very polymorphic, Gram-negative bacterium that is aerobic, generally rod-shaped, and mobilized by a single polar flagella. In previous studies, at least 10 different carbohydrate polymers (CP), including 2-40, agar, chitin, alginic acid, carboxymethylcellulose (CMC), β-glucan, laminarin, pectin, pullulan, starch and xylan It has been found that it can decompose (Ensor, Stotz et al., 1999). It has also been shown to synthesize true tyrosinase (Kelley, Coyne et al., 1990). 16S rDNA analysis showed that Teridinibacter sp. , A cellulolytic nitrogen-fixing bacterium that is a symbiotic commensal . (Distel, Morrill et al., 2002) and Microbulbifer hydrolyticus (Gonzalez and Weiner 2000), which are members of the gamma-subclass of the phylum Proteobacteria .

Agarase, chitinase and alginase systems have been characterized throughout. Zymogram activity experimental protocols indicate that all three systems consist of a plurality of depolymerases, and a plurality of lines of evidence suggest that at least some of these degrading enzymes are attached to the cell surface (Stotz 1994; Whitehead 1997; Chakravorty 1998). Activity assays show that most of the 2-40 enzymatic activity is present in the cell fraction during logarithmic growth in CP1, whereas in later growth most of the activity is found in the supernatant and cell-binding activity is dramatically reduced. (Stotz 1994). Phase growth on CP also involves dramatic changes in cell morphology. Glucose-growth cultures of 2-40 are relatively uniform in cell size and shape, generally with smooth and uncharacteristic cell surfaces. However, when grown in agarose, alginate, or chitin, 2-40 cells show new surface structures and features.

These intracellular and extracellular structures (ES) have a network of fibrillary appendages, which may be small bumps, large vesicular structures that appear to have been released from the cells, fine cilia or pili, and a type of tubule. Include. Immunoelectron microscopy shows that agarase, alginase and / or gutinase are localized to at least some types of 2-40 ES. The pattern of immunolocalization of the 2-40 enzyme to surface bumps and surface local anatomy are very similar to those seen in the cellulolytic members of the genus Clostridium .

The oldest method of studying the conversion of lignocellulosic material to sugars is based on acid hydrolysis (eg, review by Grethlein, Chemical Breakdown Of Cellulosic Materials, J. APPL.CHEM.BIOTECHNOL 28: 296-308 (1978). This process may relate to the use of concentrated or dilute acids. For example, US Pat. Nos. 5,221,537 and 5,536,325, incorporated herein by reference in their entirety, describe a two step process for acid hydrolysis of lignocellulosic material to glucose. These processes have many disadvantages, including, for example, the recovery of acids, the need for specialized materials in the structure, the need to minimize water in the system, and the high production of degradation products that can inhibit fermentation with ethanol. have.

In order to overcome the problems of acid hydrolysis processes, cellulose conversion processes have been developed using enzymatic hydrolysis. See, for example, U.S. Patent No. 5,916,780, which is incorporated herein by reference in its entirety, which destroys the integrity of the fiber structure and makes the cellulose more accessible, pretreatment step for attack by cellulose enzyme in the treatment step. And enzymatic hydrolysis is described.

US Pat. No. 6,333,181, incorporated herein by reference in its entirety, describes the production of ethanol from lignocellulosic material by treatment of a mixture of lignocellulosic, cellulose, and ethanologenic microorganisms with ultrasound. have.

Identification of enzyme systems using cellulose as a substrate, expression of genes encoding proteins using appropriate vectors, identification and isolation of amino acid products (enzymes and non-enzyme products) and preparation of ethanol as described in Bhat's literature. And for purposes such as uses, there is a need for using organisms and these products comprising these genes. There is also a need in the art to use lignocellulosic materials for the production of ethanol in order to develop more effective treatment methods resulting in higher yields of ethanol.

One aspect of the invention relates to a system of plant wall active carbohydrases and related proteins.

A further aspect of the invention relates to a process for the decomposition of a substrate comprising cellulose. This method involves contacting a cellulose-containing substrate with one or more compounds obtained from Saccharophagus degradans strains 2-40.

A further aspect of the invention relates to a group of enzymes that catalyze a reaction involving cellulose.

Another aspect of the invention relates to a polynucleotide encoding a polypeptide having cellulose degradation or cellulose binding activity.

A further aspect of the invention relates to chimeric genes and vectors comprising genes encoding polypeptides having cellulolytic activity.

A further aspect of the invention relates to a method of identifying a nucleotide sequence from S. degradans encoding a polypeptide comprising any of the following activities: cellulose degrading enzyme, or cellulose bond. The S. degradans gene library was constructed in E. coli and screened for the desired activity. Transformed E. coli cells with specific activity are generated and isolated.

Another aspect of the invention relates to a method for producing ethanol from lignocellulosic material, wherein the lignocellulosic material is treated with at least one effective saccharifying amount of the compounds listed in FIGS. 4-11 to obtain sugars. Converting the sugars to produce ethanol. The conversion and recovery of sugars to ethanol may be carried out by any established method known to those skilled in the art, but not limited thereto. For example, the use of ethanologenic microorganisms such as Zymomonas, Erwinia, Klebsiella, Xanthomonas, and Escherichia , in particular Escherichia coli K011 and Klebsiella oxytoca P2.

A further aspect of the invention includes contacting the lignocellulosic material with a microorganism that expresses one or more effective glycosylation amounts of the compounds listed in FIGS. 4-11 to obtain sugars, and converting the sugars to produce ethanol, A method for producing ethanol from lignocellulosic material.

A further aspect of the present invention is to obtain ethanol from lignocellulosic material, comprising contacting the lignocellulosic material with an ethanologenic microorganism that expresses one or more effective glycosylation amounts of the compounds listed in FIGS. 4-11 to produce ethanol. It is about how to produce. Such ethanologenic microorganisms may include an effective amount of one or more compounds listed in FIGS. 4-11 to glycosylate lignocellulosic material, and an effective amount of one or more enzymes that subsequently catalyze (separately or in concert) the conversion of sugars to ethanol or Expresses the enzyme system.

Further aspects of the present invention relate to the use of cellulose degradation substrates in food, beer, wine, animal feed, fabric preparation and laundry, pulp and paper industries, and agricultural industries.

The present invention does not penetrate lignin and / or produce ethanol including saccharification and saccharification of plant cell walls without removing or partially saccharifying hemicellulose or hemicellulose components before the cellulose-degrading enzymes can act on their substrates. It is beneficial in that a process can be obtained. The present invention also enables an ethanol production process including saccharification and saccharification, with or without high pressure, high temperature, and reduced amounts of fungal cellulase, acid (eg, sulfuric acid) in the saccharification process.

Other aspects, features, and advantages of the present invention will become apparent from the following detailed description, taken in conjunction with the accompanying drawings, which are part of the invention and which illustrate in detail the principles of the invention.

1A shows the chemical formula of cellulose.

1B shows the physical structure of cellulose.

2A shows the decomposition of cellulose fibers.

2B shows the chemical depiction of cellulose degradation into cellobiose and glucose.

3 shows SDS-PAGE and Zymogram analysis of 2-40 culture supernatants.

FIG. 4 lists the predicted cellulase of S. degradans 2-40 (the sequences from FIGS. 4-10 are each listed in SEQ ID NO: 1-214 in the order of appearance in the appendix).

FIG. 5 lists the predicted xylanase, xylosidase and related appendages of M. degradans 2-40.

6 lists the predicted pectinase and related appendages of S. degradans 2-40.

Figure 7 lists arabinanases and arabinogalactanases of S. degradans 2-40.

8 lists the metnasase of S. degradans 2-40.

9 lists lamininase of S. degradans 2-40.

10 lists selected carbohydrate-binding module proteins of S. degradans 2-40.

11 lists recombinant proteins of S. degradans 2-40 and their predictions: comparison of observed molecular weight.

Analysis of the genomic sequence of S. degradans 2-40 reveals abundance of genes encoding enzymes that are expected to degrade plant-derived carbohydrates. To date, 2-40 is the only sequenced marine bacterium with apparently complete cellulase and xylanase systems, and a number of other systems containing plant wall active carbohydrates.

Thus 2-40 play an important role in the marine carbon cycle, functioning as a "super-degrader" that mediates the breakdown of CP from various seaweed, plantal, and invertebrate sources. It seems to be possible. Significant enzymatic variability, novel surface features (ES), and apparent localization of carbohydrates against ES have been shown to allow S. degradans 2-40 to study cell biology and surface enzyme attachment of CP metabolism. Make it an interesting organism.

To degrade plant cell walls, it has now been found that 2-40 has the complete complement of properly positioned enzymes. This attempted to: a) annotation and genome analysis of the 2-40 plant-wall active enzyme system, b) identification of enzymes and other proteins comprising domains or motifs that may be associated with surface enzyme display, c) identification By the cloning and expression of a protein selected for the preparation of antibody probes, to enable the development of testable models based on the protein motifs, and d) testing of the proposed model of surface enzyme display using immunoelectron microscopy. Can be performed.

These efforts enable strategies in which genes encoding proteins with potential relevance to surface attachment can be identified based on sequence homology with modules or domains known to be functional for surface attachment and / or adhesion. This is greatly facilitated by the recent sequencing of 2-40.

Enzymatic and non-enzymatic ORFs with compelling sequence elements are identified using BLAST and other amino acid sequence alignment and analysis tools. Genes of interest can be cloned into E coli , expressed with in-frame polyhistidine affinity tag fusions and purified by nickel ion chromatography, thereby allowing for research and antibody probe production. Means for identifying and preparing recombinant 2-40 protein for the present invention.

Genomic sequences of 2-40 have recently been obtained in collaboration with the Department of Energy's Joint Genome Initiative (JGI). The completed draft sequence of January 19, 2005 contains 5.1 Mbp contained in a single contiguous sequence. Automated annotation of the Open Reading Frame (ORF) was performed by the Department of Computer Genomics at Oak Ridge National Laboratory (ORNL), and the annotated sequences were published on the World Wide Web (http://genome.ornl.gov). / microbial / mdeg).

The first genomic annotations revealed a variety of carbohydrates, including a number of agarases, alginases and chitinases. Remarkably, the genome also contains abundant enzymes with a predicted role in the degradation of plant cell wall polymers, including many ORFs homologous to cellulase, xylanase, pectinase and other glucanases and glucosidases. It includes. All in all, more than 180 open reading frames have been identified in the draft genome that have a promising role in carbohydrate catabolism.

To begin the definition of 2-40 cellulase, xylanase and pectinase systems, genes were first classified as belonging to one of those systems by BLAST homology. Ambiguous ORFs have been experimentally assigned to the most well known class of hits. Other tools used to fine-tune this experimental classification include Pfam, which uses a hidden Markov model (a statistical model of sequence concordance homology) and multiple alignments to identify the discreet modular domain in a protein sequence. Protein families database of alignments and HMMs; http://www.sanger.ac.uk/Software/Pfam/ ) and SMART (Simple Modular Architecture Research Tool; http://smart.embl-heidelberg.de/ ) . These analyzes were relatively successful; Many ORFs remain difficult to classify based on sequence homology alone.

Enzymes have traditionally been classified by substrate specificity and reaction product. Prior to the genomic era, function was considered the most obedient (and perhaps the most useful) basis for comparing enzymes, and assays for various enzyme activities have long been well developed, resulting in a well-known EC classification scheme. Other O-glycosyl hydrolases, which act on glycosidic bonds between cellulase and two carbohydrate moieties (or carbohydrate and non-carbohydrate moieties, as occurs in nitrophenol-glycoside derivatives) , EC 3.2.1.-, where the last number indicates the exact type of cleaved bond. According to this scheme, the endo-acting cellulase (1,4-β-endoglucanase) is designated EC 3.2.1.4.

With the ease of nucleotide sequencing of cloned genes and the emergence of well-known genomic sequencing projects, the increased amounts of sequence data facilitated the comparison and analysis of related genes and proteins on an unprecedented scale. This is especially true for carbohydrates; E.C. As can be seen in the nomenclature scheme, it has been clarified that the classification of such enzymes by reaction specificity is limited by their inability to convey sequence similarity. In addition, an increasing number of carbohydrates crystallized and their 3-D structures were revealed.

One of the major revelations of carbohydrate sequence and structural analysis is that there is a discreet family of enzymes with related sequences that include conserved three-dimensional folds that can be predicted based on their amino acid sequence. Is the point. It has also been found that enzymes with the same three-dimensional folds exhibit the same stereospecificity of hydrolysis, even if they catalyze different reactions (Henrissat, Teeri et al. 1998; Coutinho and Henrissat 1999).

These findings are available at http://afmb.cnrs-mrs.fr/CAZY/index.html (Coutinho and Henrissat 1999; Coutinho and Henrissat 1999), carbohydrates available in the form of an Internet database, Carbohydrate-Active enZYme server (CAZy). It forms the basis of sequence-based classification of degrading enzyme modules.

CAZy has four major classes of carbohydrases: glycosyl hydrolase (GH's), glycosyltransferase (GT's) and polysaccharide degrading enzymes (PL's), based on the type of catalyzed reaction. And carbohydrate esterases (CE's). GH's cleave glycoside bonds through hydrolysis. This case includes many well known polysaccharidases such as cellulase, xylanase, and agarase. GT's generally function in polysaccharide synthesis and catalyze the formation of new glycoside bonds through the transfer of sugar molecules from activated carrier molecules to receptor molecules, such as uridine diphosphate (UDP). While GT's often function in biosynthesis, there are examples where mechanisms for binding cleavage are used, as occurs in phosphorolytic cleavage of cellobiose and cellodextrins (Lou, Dawson et al. 1996). PL's use a β-removal mechanism to mediate bond cleavage and are commonly associated with alginate and pectin depolymerization. CE's generally act as deacetylases on O- or N-substituted polysaccharides. Typical examples include xylan and chitin deacetylases. Sequence-based families are designated by numbers within each class, as seen in GH5: glycosyl hydrolase family 5. Members of GH5 hydrolyze β-1,4 bonds in a retaining fashion, using a double-displacement mechanism that results in retention of the original binding stereospecificity. The maintenance or reversal of the anomeric configuration is a general property of a given GH family (Henrissat and Bairoch 1993; Coutinho and Henrissat 1999). Many examples of endocellulase, xylanase and mannanase belonging to GH5 have been reported to exhibit the various substrate specificities possible within the GH family. In addition, GH5 is dominated by endohydrolases, which cleave the chains of their respective substrates at random locations within the polymer chains. This is true for GH5, but this generalization is not true for many other GH families. In addition to carbohydrates, the CAZy server defines many families of Carbohydrate Binding Modules (CBMs). Like the catalytic module, the CBM family is designated based on amino acid sequence similarity and conserved three-dimensional folds.

The CAZyme structural family was introduced into a new classification and naming scheme developed by Bernard Henrissat and his colleagues (Henrissat, Teeri et al. 1998). Traditional gene / protein nomenclature assigns acronyms that indicate general function and order of discovery; In this scheme, the cellulose gene of an organism is defined as celA, celB, etc., regardless of the actual mechanism of action in cellulose. Some researchers have attempted to convey more information by naming cellulase, such as endoglucanase (engA, engB) or cellobiohydrolases (cbhA, cbhB), but this requires determination of function in vivo and However, there is still a failure to convey the relevance of protein sequences and structures. CAZyme nomenclature holds the familiar acronyms to indicate the functional system in which the gene belongs to family number designation and is introduced. The uppercase letters after the family number indicate the order of reporting within a given organism system. CeHulomonas fimi. Examples are given by two endoglucanases of CenA and CenB. In the old nomenclature, nothing could be inferred from the name except in the order of discovery. Naming them, respectively, Cel6A and Cel9A, immediately clarifies that these two cellulases are irrelevant in sequence and thus belong to different GH families (where Cel represents cellulose and 9 is glycosyl hydrolase). Family 9). These schemes do not distinguish between endo- and exo-activity, but these assignments are not absolute and may be included in the discussion of enzymes when relevant (ie, cellobiohydrolase Cel6A, endoxylanase Xyn1OB). The catalyst module takes precedence over naming carbohydrates; Since many (or most) carbohydrates contain at least one CBM, they are named for an enzymatic module. If more than one catalytic domain is present, they are named in order from the N-terminus to the C-terminus, ie cel9A-cel48A comprises GH9 at the amino terminus and GH48 at the carboxy terminus. Both domains act on cellulose. However, there are many examples of CBM modules that occur on proteins without any predicted carbohydrate module. In the absence of some other predicted functional domains (such as proteases), these proteins are termed the CBM module family. When multiple CBM families are present, they are again named amino to carboxy terminus, ie cbm2D-cbm10A (Henrissat, Teeri et al. 1998). This nomenclature has been widely accepted and will be used for the naming of all 2-40 plant-wall activated carbohydrates and related proteins considered as part of this study.

The cell walls of higher plants consist of various carbohydrate polymer (CP) components. These CPs interact through covalent and non-covalent means to form a rigid cell wall and provide the structural integrity required for plants to withstand expansion pressures. The main CP found in plants is cellulose, which forms the structural backbone of the cell wall. See FIG. 1A. During cellulose biosynthesis, the chains of poly-β-1,4-D-glucose associate themselves through hydrogen bonding and hydrophobic interactions to form cellulose microfibers which further associate themselves to form larger fibers. Cellulose microfibers are rather irregular and contain varying crystalline regions. The degree of crystallinity of cellulose fibers depends on how tightly the hydrogen bonds between their component cellulose chains are arranged. Regions with less-arranged bonds, thus more accessible glucose chains, are referred to as amorphous regions (FIG. 1B). Relative crystalline diameter is a characteristic of the biological source of cellulose (Beguin and Aubert 1994; Tomme, Warren et al. 1995; Lynd, Weimer et al. 2002). Irregularity of cellulose fibers results in steric effects that hinder the access and subsequent degradation of the enzyme and a wide variety of varied bonding angles.

A general model for cellulose depolymerization to glucose is associated with at least three separate enzyme activities (see Figures 2A and 2B). Endoglucanases cleave cellulose chains internally, creating shorter chains and increasing the number of accessible ends acted by exoglucanase. These exoglucanases are specific for the reducing or non-reducing end and often liberate cellobiose, dimers of cellulose (cellobi hydrolases). Increasing cellobiose is cleaved into glucose by cellobiase (β-1,4-glucosidase). In many systems, additional types of enzymes are present: Cellodextrinases are β-1,4-glucosidases that cleave glucose monomers from cellulose oligomers, not from cellobiose. Because of the many crystalline and structural complexities of cellulose, and the enzymatic activity required, degradation, organisms with a "complete" cellulase system can produce a variety of endo and / or exo-functional β-1,4-glucanases. Synthesize

For example, Cellulomonas fimi and Thermomonospora fusca have been shown to synthesize six cellulase, respectively, while Clostridium thermocellum has been shown to have more than 15 (Tomme, Warren et al. 1995). Perhaps a change in the shape of the substrate-binding pocket and / or active site of many of these cellulases facilitates complete cellulose degradation (Warren 1996). It is contemplated that organisms with complete cellulase systems will be able to efficiently use plant biomass as a carbon and energy source while mediating cellulose degradation. Although many of these are thought to function as members of a consortia (such as the ruminal community) that can collectively achieve total or near-total cellulose hydrolysis, the ecology of an incomplete cellulose system The scientific and evolutionary roles are less clear (Ljungdahl and Eriksson 1985; Tomme, Warren et al. 1995).

In plant cell walls, microfibers of cellulose are incorporated into a matrix of hemicelluloses (including xylan, arabinan and mannan), pectin (galacturonan and galactan), and various β-1,3 and β-1,4 glucans. Is embedded. These matrix polymers are often substituted with arabinose, galactose and / or xylose residues to obtain arabinoxsilane, galactomannan and xyloglucan-designating several (Tomme, Warren et al. 1995; Warren 1996; Kosugi; , Murashima et al. 2002; Lynd, Weimer et al. 2002). The complete sheer number and complexity of the different glycosyl bonds presented by these non-cellulosic CPs require specific enzyme systems that often compete with cellulase systems in enzyme counts and complexities. Because of its heterogeneity, plant cell wall degradation often requires a community of microorganisms (Ljungdahl and Eriksson 1985; Tomme, Warren et al. 1995).

-S. Desired degradans and M degradans synthesize a complete multi-enzyme system that degrades the major structural polymers of plant cell walls. A) defining cellulase and xylanase systems that determine the activity of genes whose function cannot be predicted by sequence homology; And B) identification and annotation of genomes of other plant-degrading enzyme systems by sequence homology (ie, pectinase, lamininase, etc.).

All patent publications, patents, and patent applications are hereby expressly incorporated by reference in their entirety to the same extent as if each individual patent publication, patent or patent application is clearly and individually incorporated by reference in its entirety. do.

The following examples are illustrative and are not intended to limit the scope of the invention in any way.

Experiment result

I: Genomic, Proteomic and Functional Analysis of 2-40 Plant-Wall Active Enzymes

From the ORNL annotation, it is clear that the 2-40 genome contains many enzymes with predicted activity on plant cell wall polymers. This is particularly surprising because 2-40 is an estuary-like bacteria in the estuary that has several complex enzyme systems that degrade conventional marine polysaccharides such as agar, alginate, and chitin. Defining a multienzyme system based on automated annotation is complex due to the presence of poorly conserved domains and / or new combinations of domains. There are many examples of this in 2-40 plant-wall active enzymes. Thus, ORNL annotations of carbohydrate ORFs are observed manually with a focus on module composition, and are then assigned to a general group based on substrates that are likely to be involved (ie cellulose or xylan degradation). These genomic sequencing led to a pool of about 25 potential cellulase, 11 xylanases and 17 pectinase.

When sequence homology is well preserved, highly accurate predictions of function are possible. Thus, to confirm the presence of functional cellulase and xylanase systems in M degradans , a zymogram and enzyme activity assay was performed as described below. In addition, attempts were made to identify enzymes from 2-40 culture supernatants using Mass Spectrometry based proteomics.

Next, more sophisticated genomic analysis was used, if any, to identify ORFs that require functional characterization to determine their role in cellulase and xylanase systems and to predict function where possible. Based on sequencing and B. Henrissat's functional predictions, ORFs belonging to different plant wall-active enzyme systems were experimentally classified.

To gain insight into the induction and expression of 2-40 cellulase and xylanase, Avicel (DNA) assay was performed by dinitrosalicylic acid reducing-sugar assay (DNSA analysis) as reviewed in the Experimental Protocols section at the end of this proposal. specific activity was measured for avicel) and xylan-growing cells and supernatants. In order to investigate possible co-induction of activity by these two substrates co-occurring in the plant cell wall, xylanase activity was measured for Avicel-growth cultures and vice versa.

Growth in Avicel or xylan resulted in enzymatic activity for both substrates and indicates co-induction of cellulase and xylanase systems. The highest level of activity is induced by homogenous substrates, along with other 2-40 carbohydrate systems. The results also reveal some important differences in the expression of these two systems. When grown in Avicel, cellulase activity is cell-associated in early growth and significantly accumulated in later stage supernatants. Cell and supernatant fractions exhibit low levels of xylanase activity, which are almost equally present throughout all growth stages. In contrast, xylan-growth cultures exhibit most of the xylanase and cellulase activity in cell fractions throughout the growth cycle. Cellulase activity does not accumulate in the supernatant, xylanase activity accumulates moderately, but still remains under cell-adhesion activity.

Enzymatically active gels (Zymograms) of Avicel and xylan growing cell pellets and culture supernatants were analyzed to visualize and confirm expressed cellulase and xylanase. Zymograms revealed five xylan hydrolyzable bands in xylan-growth supernatants (FIG. 3), four of which corresponded well to the calculated MW of predicted xylanase (xyl / arb43G-xyn10D : 129.6 kDa, xyn1OE: 75.2 kDa, xyn1OC: 42.3 kDa, and xyn11A: 30.4 kDa; see FIG. 2). Avicel-growth cultures showed MW and 8 active bands in the 30-15OkDa range in the CMC zymogram. CMC is generally a suitable substrate for endocellulase activity. These zymograms clearly demonstrate that during growth in Avicel 2-40 synthesizes a number of endocellulases of varying sizes-indicating that it is a functional polyenzyme cellulase system. The CMC and xylan zymogram together confirm the results of inducible expression and genomic analysis of the multienzyme cellulase and xylanase system at M degradans 2-40.

Culture supernatants using reverse phase high performance liquid chromatography (RP-HPLC) combined with tandem mass spectrometry (MS / MS) to identify individual cellulase and xylanase generated during CP phase growth Proteomic analysis was performed. Prior to electrospray ionization and MS / MS analysis, the power from separation of peptides on RP-HPLC columns allows the identification of large numbers of proteins from complex samples (Smith, Loo et al., 1990; Shevchenko, WiIm et al. 1996; Jonsson, Aissouni et al. 2001). These analyzes confidently identified more than 100 different non-enzymatic proteins and a number of carbohydrates such as xylanase, two xylosidases, cellulases, and two cellodedextrinases. Agarase was identified during further analysis of the agarose-growth supernatant.

Experimental protocol-slice digestion, extraction, and MS / MS analysis performed on a Stanford University mass spectroscopy facility identified two annotated cellulase from Avicel-growth supernatant samples. One designated cel5H was identified from the band with a predicted MW of 67 kDa and an apparent MW of 75 kDa. The other, cel9B, had a predicted MW of 89 kDa, but had an apparent MW of 120 kDa. The discrepancy between the predicted and apparent MW of cel9B is consistent with a similar case in which some 2-40 proteins cloned and expressed in E coli show 30-40% more apparent MW than their predicted MW.

The amino acid translocations of all genetic models in the 2-40 draft genome were analyzed on a CAZy ModO (Carbohydrase Active enZyme Modular Organization) server in AFMB-CRNS. This analysis identified all genetic models including catalyst modules (GH GT, PL, or CE) and / or CBM. All in all, the genome included 222 gene models containing the CAZy domain, most of which have a modular architecture. Of these, 117 include GH modules, 39 include GT, 29 include PL, and 17 include CE. Many of these have more than one CBM from various families. There are also 20 proteins that contain CBM but do not contain the predicted carbohydrate domain.

Detailed comparison of the 2-40 module sequences to those in the ModO database allowed specific predictions of the function of the modules with highly conserved sequences of active sites. For example, Cel9B (from experimental protocol slice MS / MS) includes the GH9 module, CBM2 and CBM10 module, which are expected to function as endocellulase.

When the catalyst module sequence is less conserved, only the overall mechanism can be predicted. The same is true for gly5M containing GH5, which is expected to be 1,3 or 1,4 glucanase, and because the sequence analysis is unknown, the acronym designation "gly" was written for the glycanase.

The results of this detailed evaluation and analysis were used to assign genes to the cellulase, xylanase, pectinase, lamininase, arabinase and mannanase systems. Each system is also assigned a corresponding accessory enzyme, ie the cellobiase belongs to the cellulase system and the xylosidase belongs to the xylanase system. Genes with less conserved GH modules with the greatest likelihood of functioning as cellulase, xylanase or adjuncts were identified and designated as needed for demonstration of function.

The results of the ORNL annotation, subsequent annotation analysis, proteomic (mass spectrometry) analysis, CAZyme module analysis, and functional prediction include a table summarizing the genes and predicted plant wall active carbohydrates of only 2-40 selected CBMs. Was introduced in Figures 4-11.

Genes selected for cloning and functional analysis include carbohydrates gly3C, gly5K, gly5M, gly9C, and gly43M. Since the active site of gly5L is very homogeneous with the active site of gly5K, its activity is inferred from the results obtained from gly5K. Four of the 20 “CBM only” proteins, cbm2A, cbm2B, cbm2C and cbm2D-cbm10A, are included in the activity assay to investigate the predicted lack of enzyme function. These four contain CBM2 modules that are expected to bind to crystalline cellulose. This predicted affinity is why they are included in activity assays, and such proteins that bind cellulose are likely to include cellulase or xylanase modules that are not detected by sequencing. With CBM only protein, the lack of detected enzyme activity will confirm the absence of the catalytic domain (CD).

In order to define the complete cellulase and xylanase system of M degradans , such enzymes belonging to the system but which cannot be assigned with confidence based on sequence homology, are described in terms of activity, as described in expression, purification and experimental protocols. Will be analyzed. To date, gly3C, gly5K, gly5M, gly9C and gly43M and cbm2A, cbm2B, cbm2C and cbm2D-cbm10A have been cloned into expression strains as pETBlue2 (Novagen) constructs. This vector positions expression under the control of the inducible T7 lac promoter and introduces a C-terminal 6xHistidine tag, allowing purification of the recombinant protein by nickel ion affinity. Successful cloning and expression of these proteins was confirmed by Western blot using α-HisTag ^® monoclonal antibody (Novagen). All expressed proteins had clear MWs close to or greater than their predicted MWs, except for Cbm2D-CbmlOA, which appeared to be unstable (Table 8); Two separate attempts to clone and express a protein is brought a HisTag ^® including bands that occurred near the dye front (dye front) in the Western blot, suggesting that the protein degradability (proteolytic) degradation of these gene products. An additional enzyme, Cel5A, was cloned and expressed for use as an endocellulase positive control in the activity assay. Cel5A has a predicted MW of 129 kDa, contains two GH5 modules, and is also highly active in the HE-cellulose zymogram.

The primary criterion for assigning a function will be the substrate on which it is acted and the type of activity detected. As such, various enzyme activity assays will focus on providing qualitative demonstration of function rather than rigorous quantification of relative activity levels. The required assay is dictated by the substrate being tested and described in more detail in the experimental protocol. With respect to cellulose, β-1,4-endoglucanase (endocellulase), β-1,4-exoglucanase (cellobiohydrolase), and β-1,4-glucosidase (cell Roviase) It is important to distinguish between active. This is a zymogram for endocellulase, a DNSA reduction-sugar assay for cellobiohydrolase, and p-nitrophenol-β-1,4-cellobiside (pnp-cellobiose for cellobiase activity). ) Was performed. The combined results from all three assays will enable the definition of function as follows: Positive zymograms show endocellularase activity and negative zymograms combined with positive DNSA assay and negative pnp-cellobiose assay Represents exocellulases, while DNSA and negative zymograms with positive pnp-cellobiose results will mean that the enzyme is a cellobiase.

Xylanase (β-1,4-xylanase), lamininase (β-1,3-glucanase), and mixed glucanase (β-1,3 (4) -glu Canase) activity will be determined by xylan, laminarin and barley glucan zymogram, respectively. Unlike cellulose, no reports of "xylobiohydrolase" or any other exo-acting enzyme appear that specifically cleave dimers from these substrates. Therefore, the zymogram will be sufficient to demonstrate the degrading enzyme (endo) activity, and the pnp-derivative will detect monosaccharide (exo) cleavage. The pnp-derivatives used in this study were pnp-α-L-arabinofuranoside, -α-L-arabinofyranoside, -β-L-arabinofyranoside, -β-D-cellobi It will include an oxide, -α-D-xycopyyranoside, and -β-D-xylopyranoside. These substrates were selected based on the possible activity of the domain in question. This assay includes any α- and β-arabinosidase, β-cellobiase, β-xylosidase, bifunctional α-arabinosidase / β-xylosidase, and α- It will be possible to measure the function for xyloxysidase, which cleaves the α-linked xylose from xyloglucan. pnp-derivative assays will be performed in 96-well microtiter plates using standard curves of p-nitrophenol concentrations, as described in the experimental protocol.

β-1,4-, β-1,3-, and β-1,3 (4) -glucanase activity, and β-1,4-xylanase and various exo-glycosidase activities The combination of assays for can clearly analyze the function of the ambiguous carbohydrate. Proteins with proven activity will be assigned to the appropriate enzyme system.

Experimental protocol

Zymogram

All active gels were prepared as standard SDS-PAGE gels with appropriate CP substrates introduced directly into the separation gel. Zymograms are cast into substrates dissolved in 8% polyacrylamide concentration and dH ₂ O and / or gel buffer solution to yield 0.1% (HE-cellulose), 0.15% (barley β-glucan), or 0.2% Final concentration of silane). Gels were used under discontinuous conditions according to the procedure of Laemmli 1970, except for 8 min treatment at 95 ° C. in sample buffer with a final concentration of 100 mM dithiothreitol (DTT) and 2% SDS. After electrophoresis, the gels were incubated for 1 hour at room temperature in 80 ml of regeneration buffer of 20 mM PIPES buffer pH 6.8 containing 2.5% Triton X-100, 2 mM DTT and 2.5 mM CaCl ₂ . Calcium was included to aid in the refolding of potent calcium-binding domains such as tsp3s of Lam16A.

After 1 hour equilibration, the gel was placed in a fresh 80 ml portion of the regeneration buffer and kept overnight at 4 ° C. with gentle vibrations. The next morning the gel was equilibrated to 80 ml of 20 mM PIPES pH6.8 for 1 hour at room temperature, transferred to a clean container, covered with a minimum amount of PIPES buffer and incubated at 37 ° C. for 4 hours. After incubation, the gel was stained for 30 minutes with a solution of 0.25% Congo red in dH ₂ O (HE-cellulose, β-glucan and xylan) or 0.01% Toluidine blue in 7% acetic acid. . The gel was destained with 1M NaCl for Congo Red and dH ₂ O for toluidine blue until a clear band was observed against the stained background.

Nelson-Somogyi Reduction-Sugar Analysis

Purified proteins were assayed for activity using a 50 Oul reaction volume using a change in the Nelson-Smoke Reduction Sugar method applied on 96-well microtiter plates (Green, Clausen et al. 1989). Test substrates include Avicel, CMC, Phosphoric Acid Swollen Cellulose (PASC), Barley Glucan, Laminarin, and Xylene dissolved in 1% (Barley Glucan and Laminarin, 0.5%) in 20 mM PIPES pH 6.8. It was. Barley glucan, laminarin and xylan assays were incubated at 37 ° C. for 2 hours; Avicel, CMC and PASC assays were incubated at 37 ° C. for 36 hours. Samples were analyzed in triplicate, corrected for blank values, and levels were estimated from standard curves. Protein concentrations of the enzyme assay samples were obtained in triplicate using the Pierce BCA protein assay according to the manufacturer's instructions. Enzyme activity was calculated in one unit (U), defined as 1 μM released reduced sugars / min and reported as specific activity with U / mg protein.

Exoglycosidase Activity Assay: pnp-derivative

α-L-arabinofuranoside, -α-L-arabinofyranoside, -β-L-arabinofyranoside, -β-D-cellobioside, -α-D-glucopyrano Purified proteins were analyzed for the activity on pNp derivatives of seeds, -β-D-glucopyranoside, -α-D-xycopyyranoside and -β-D-xyclopyranoside. 25 μl enzyme solution was added to 125 μl 5 mM substrate solution in 20 mM PIPES pH 6.8, incubated at 37 ° C. for 30 minutes, and A ₄₀₅ measured. After correcting for the blank reaction, the readings are compared to the p-nitrophenol standard curve and specific activity as U / mg protein in one unit (U), defined as 1 μmol p-Np / min. (specific activity).

Mass spectroscopy and proteomic analysis

Stationary-phase supernatants from Avicel, CMC, and xylan-grown cultures are concentrated 25-fold by centrifugal force ultrafiltration using a microcon or centricon device (Millipore) It was. Sample protein concentration was measured by BCA protein analysis. Samples were exchanged with 100 mM Tris buffer, pH 8.5, which also contained 8M urea and 10 mM DTT. Samples were incubated with stirring at 37 ° C. for 2 hours to denature proteins and reduce disulfide bonds. After reduction, 1 M iodine acetate was added to a final concentration of 50 mM and the reaction was incubated for 30 minutes at 25 ° C. in the dark. This step alkylates the reduced cysteine residues, thereby preventing the reformation of disulfide bonds. The sample was then exchanged to 50 mM Tris, 1 mM CaCl ₂ , pH 8.5 using a microcon apparatus. Denatured, reduced and alkylated samples were digested into peptide fragments using proteomics-grade trypsin in a 1:50 enzyme (trypsin) to substrate (supernatant) ratio. Typical digestion reaction was about 150 μl total volume. Digestion was incubated overnight at 37 ° C., stopped by addition of 99% formic acid to a final concentration of ˜1% and analyzed by RPHPLC-MS / MS at UMCP College of Life Sciences CORE mass spectroscopy equipment.

Peptide fragments were loaded onto a Waters 2960 HPLC fitted with a 12 cm microbore column containing C18 as adsorbent and eluted with a linear gradient of increasing acetonitrile (CH3CN) concentration with an electrospray ionizer. It was. The electrospray device ionized the peptide and injected it into a Finnagin LCQ tandem mass spectrometer. Automated operating software controlled the solvent gradient and continuously scanned the eluted peptides. The program fragmented them by identifying each of the three most abundant ionic species in the irradiation scan, separating each of them from the ion trap of the mass spectrometer, and inducing collisions with helium molecules. The resulting sub-fragment mass was recorded for further analysis by peptide analysis packages such as SEQUEST and MASCOT. After three subscan and crash cycles were completed, the MS received another irradiation scan and repeated the cycle until the end of the operation, typically about 3 hours. Raw MS reads are used by analysis software to generate peptide fragment sequences that are compared to amino acid sequence translocations of all genetic models in the 2-40 draft genome. Peptide identity matches were assessed using the accepted threshold of statistical significance specific to each program.

Cloning and Expression of 2-40 Protein in E coli

The basic cloning and expression system used pETBlue2 (Novagen) as the vector, E coli DH5α (Invitrogen) as the cloning strain, and E coli BL-21 (DE3) Tuner® cells (Novagen) as the protein expression strain. This system enables cloning of virulence or other different genes, because the vector is expressed under the control of the -T7 lac promoter lacking in cloning strain DH5α, thereby abolishing even low levels of expression during plasmid screening and propagation. Because. After blue / back screen, plasmids were purified from DH5α and transformed into expression hosts (Tuners). The Tuner strain has a T7 lac promoter, enables IPTG-inducible expression of vector-encoded protein, and lacks Lon and Omp proteases.

The nucleotide sequence of the genetic model was obtained from DOE JGI's Microbulbifer degradans genomic web server and placed in the PrimerQuest ™ design tool at the Integrated DNA Technologies web page at http://biotools.idtdna.com/Primerquest/ . Design parameters were Optim Tm 6O ° C., Optimal Primer Size 20nt, Optimal GC% = 50, with primers at the first and last 100 nucleotides of each ORF to clone as much of the gene as possible. The product size range was chosen to be selected. PETBlue2, a cloning and expression vector, provides start and stop codons for C-terminal 6 × Histidine fusion and protein expression. Therefore, careful attention to the frame of the vector and insertion sequence is required when adding 5 'restriction sites to PCR primers. The resulting "tailed primers" are 26-30 nts long and their sequences are "virtual cloning" analysis using the PDRAW software package (http://www.acaclone.com). It confirmed by. This program allows the vector and insert DNA sequences to be cleaved with standard restriction enzymes and ligated together. The amino acid translocations of the resulting sequence were examined to detect any frame shifts that were mistakenly introduced in the primer design. After this inquiry, primers were purchased from Invitrogen (Frederick, MD).

PCR reactions were performed with 0.5 μl 2-40 genome DNA and 10 μM forward and reverse primers, 1 μl 10 mM DNTPs, 1.5 μl 100 mM MgCl ₂ , and 1 μl Proof Pro® Pfu polymerase (50 μl) as a template. Included. PCR conditions used standard parameters for Pfu DNA polymerase and primers with tails. PCR products were cleaned up with a QIAGEN QIAquick PCR Cleanup Kit and observed on a 0.8% agarose gel. After confirmation of cleaning and size, the PCR product and pETBlue2 a suitable restriction enzyme, typically cut with Ascl and Clal for 1 to 4 hours at 37 ℃ and clean it by using the QIAquick kit, which was observed in the agarose gel. Clear digestions were ligated using T4 DNA ligase for at least 2 hours in the dark at room temperature. The ligation was then transformed into E coli DH5α by electroporation. Transformants were incubated for one hour at 37 ° C. in non-selective media and then plated onto LB agar containing ampicillin and X-gal. As pETBlue2 carries the Amp ^r gene and the insert is cloned into the lacZ ORF, the white colony contains the insertion sequence. White colonies were picked with toothpicks, patched onto fresh LB / Amp / X-gal plates, and three of the patched colonies were used to inoculate 3 ml overnight liquid medium. Plasmids were prepared from liquid media corresponding to patched colonies that remained white after overnight outgrowth. These plasmid preparations were then cleaved alone with appropriate restriction enzymes and visualized by agarose electrophoresis for size determination.

The plasmid was then heat-shock transformed with the Tuner® strain carrying the chromosome chloramphenicol resistance gene (Cm ^r ). Transformants were incubated for 1 hour at 37 ° C. in non-selective rescue media, plated on LB agar with Amp and Cm (Tuner medium) and incubated at 37 ° C. overnight. Any colony so selected will include the vector and the insert. This was confirmed by patching 3 colonies onto Tuner medium plate and inoculating the corresponding 3 ml overnight liquid medium. The next morning the 25 ml liquid medium was inoculated using the liquid medium, which grew to OD _60O near 0.6 (2-3 hours). At this time 1 ml aliquots were removed from the culture, pelleted and resuspended in 1/10 volume 1 × SDS-PAGE treatment buffer. This pre-derived sample was frozen at −20 ° C. for later use in western blots. The remaining liquid medium was then corrected with 1 mM IPTG and incubated at 37 ° C. for 4 hours. Induced pellet samples were collected at time intervals. These samples and pre-derived controls were run on a standard SDS-PAGE gel and electroblotted onto PVDF membranes. The membranes were then treated as Western blot using a 1/5000 dilution of monoclonal mouse α-HisTag® primary antibody followed by HRP-conjugated goat α-mouse IgG secondary antibody. Bands were colorimetrically visualized using the BioRad's Opti-4CN Substrate Kit. Although not present in the uninduced control, the presence of a band of His tag in the derived sample confirmed successful expression, and comparison of the band from the time point of the time interval was used to optimize later induction parameters, larger-scale purification. Was used.

Preparation and Purification of Recombinant Proteins

Expression strains were grown to OD _60O of 0.6 to 0.8 in 500 ml or 1 liter liquid medium of tuner medium. Non-derived samples were collected at this time and the remaining cultures were induced to a final concentration of 1 mM by addition of 100 mM IPTG. Induction was carried out at 37 ° C. for 4 hours or at 25 ° C. for 16 hours. Culture pellets were harvested and frozen overnight at −20 ° C. to aid storage and cell lysis. The pellet was then melted on ice for 10 minutes, transferred to a pre-weighed falcon tube and weighed. The cells were then shaken for 1 hour at 25 ° C. in ₄ ml of lysis buffer (8 M Urea, 10 mM NaH ₂ PO ₄ , 25 mM Tris, pH 8.0) per gram wet pellet. Lysates were centrifuged at 15,00 g for 30 minutes to form cell debris. The cleared lysate (supernatant) was pipetted into a clean Falcon tube where 1 ml of QIAGEN 50% Nickel-NTA resin was added to each 4 ml of cleared lysate. The mixture was stirred gently for 1 hour at room temperature to facilitate binding between dendritic Ni ⁺² ions and His tags of the recombinant protein. After binding, the slurry was loaded into a disposable mini column, flow thru (degraded lysate) was collected and stored for later evaluation. The resin was washed twice with lysis buffer adjusted to pH 7.0; Each volume of these washes was the same as the original volume of clarified lysate. Flow throughs of these two washes were also stored for subsequent analysis in Western blot to assess purification efficiency.

The column then contains a relatively purified recombinant protein fixed by His tag at their C-terminus. This is an ideal situation for refolding, where the column moves to a 4 ° C. chamber, and a series of regeneration buffers with decreasing urea concentration pass through the column. The regeneration buffer contains varying amounts of urea at 25 mM Tris pH 7.4, 50 mM NaCl, and 20% glycerol. This buffer is prepared as a stock solution containing 6M, 4M, 2M and 1M urea. Their aliquots can be easily mixed to obtain 5M and 3M urea concentrations, thus providing a series of urea concentrations descending in the 1M steps. One volume of 6M buffer (original lysate volume) passes through the column, followed by one volume of 5M buffer, followed by 1M buffer-which is repeated once to ensure column equilibrium in the 1M element. The refolded protein is then eluted at 8 fractions of 1/10 ^th original volume using 1M urea, 25 mM Tris pH 7.4, 50OmM NaCl, 20% glycerol, including 25OmM imidazole. Imidazole disrupts Nickel ion-His tag interactions, thereby releasing proteins from the column.

Western blot was used to assess the amount of His tagged protein in the eluted fractions, both washes and degraded lysate. If abundant recombinant protein is present in the degraded lysate and / or wash, it is possible to repeat the process and "scavenge" more protein. The eluate fractions containing the protein of interest were pooled and then concentrated and exchanged into storage buffer (20 mM Tris pH 7.4, 10 mM NaCl, 10% glycerol) using a Centricon centrifugal ultrafiltration device (Millipore). . Enzyme preparations were then aliquoted and frozen at −80 ° C. for use in activity assays.

In various embodiments of the invention, the cellulolytic enzymes of the invention, including related proteins and systems, including, for example, one or more enzymes or cellulose-binding proteins, have many uses. Many possible uses of the cellulase of the present invention are the same as described for other cellulase in the cell "Cellulases and related enzymes in biotechnology" by MK Bhat (Biotechnical Advances 18 (2000)), the contents of which are incorporated herein by reference in their entirety. Is integrated into. For example, the cellulase of the present invention and its systems can be used in food, beer, wine, animal feed, textile manufacturing and laundry, pulp and paper industry, and agricultural industry.

In one embodiment, three systems can be used to break down cellulose to make short chain peptides for use in medicine.

In another embodiment, three systems are used to extract olive oil in the extraction and / or purification of fruit and vegetable juices, in the manufacture and storage of fruit nectar and puree, in the flavor and other sensory properties of food, in altering the fabric, In the improvement of the quality of bakery products, in beer brewing and wine making, in the manufacture of monogastic and ruminant feeds, defibrillation of lyocells, laundry garments and the like, " Fading " destroys cellulose in fabric and laundry techniques including denim materials, in the manufacture of paper and pulp products, and in agricultural applications.

In some embodiments of the invention, cellulose can be used to absorb environmental contaminants and waste debris. Cellulose can then be degraded by the cellulase degradation system of the present invention. Bacteria that can metabolize environmental contaminants and break down cellulose can be used in bioreactors that break down toxic substances. Such bioreactors would be advantageous because there would be no need to add additional nutrients to keep the bacteria-they would use cellulose as the carbon source.

In some embodiments of the invention, the cellulolytic enzyme system may be provided in dry form, in a buffer, as a paste, paint, micelle, and the like. Cellulolytic enzyme systems may also include additional components such as metal ions, chelators, detergents, organic ions, inorganic ions, additional proteins such as biotin and albumin.

In some embodiments of the present invention, the cellulose degradation system of the present invention may be applied directly to cellulose materials. For example, a system comprising one, some, or all of the compounds listed in FIGS. 4-11 can be applied directly to a plant or other cellulose containing item such that the system degrades the plant or other cellulose containing item. As another example, 2-40 can be grown on a plant or other cellulose containing item, which causes 2-40 to prepare the compounds listed in FIGS. 4-11 to degrade the cellulose containing item as 2-40 grows. Can make it possible to do. An advantage of using 2-40 or the system of the present invention is that decomposition of cellulose containing plants or items can be carried out in an oceanic environment, for example in water.

Nucleotides with homology selected from 100%, 99%, 98%, 97%, 96%, 95%, 90%, 85%, 80%, or 75% for any of the sequences of compounds listed in FIGS. 4-11 Providing a sequence is an aspect of the present invention.

The invention also relates to non-natural or non-standard nucleotides, such as phosphorothioate, deoxyinosine, deoxyuridine, isocitosine, of 1-20 nucleotides of any sequence of the compounds listed in FIGS. 4-11. , Substitutions with ribonucleic acids including isoguanosine, 2- O -methyl, and substitutions of phosphodiester backbones with, for example, alkyl chains, aryl groups, and protein nucleic acids (PNAs).

Fig. 4 under stringent conditions of 1 x SSC, 2 x SSC, 3 x SSC1, 4 x SSC, 5 x SSC, 6 x SSC, 7 x SSC, 8 x SSC, 9 x SSC, or 10 x SSC. Providing a nucleotide sequence that hybridizes to any one of the compounds listed in -11 is another aspect of some embodiments of the invention.

The scope of the present invention covers the natural and unnatural alleles of any one sequence of the compounds listed in FIGS. 4-11. In some embodiments of the invention, alleles of any one sequence of the compounds listed in FIGS. 4-11 are similarly charged of one, two, three, four, or five naturally occurring amino acids. Substitutions with charged, shaped, sized, or situated amino acids (conservative substitutions). The invention also relates to non-natural or non-standard amino acids such as selenocysteine, pyrrolysine, 4-hydroxyproline, 5-hydroxylysine, phosphoserine, phosphotyrosine, and D- of 20 standard amino acids. Cover the isomers.

Some embodiments of the invention are directed to treating lignocellulosic material with an effective glycosylation amount of one or more of the compounds listed in FIGS. 4-11, preferably the cellulase cel5A listed in FIG. To a process for preparing ethanol from lignocellulosic material, comprising converting a saccharide to a saccharide. The treatment can be carried out in a marine environment, such as in water. One or more of the compounds listed in FIGS. 4-11 may be present in dry form, in a buffer, or in the form of a paste, paint, or micelle.

The conversion and recovery of sugars to ethanol can be carried out by any well-established method known to those skilled in the art, but not limited thereto. For example, ethanologenic microorganisms such as Zymomonas, Erwinia, Klebsiella, Xanthomonas , and Escherichia, preferably Escherichia coli K011 and Klebsiella oxytoca P2 are used.

In a further aspect of the invention, the lignocellulosic material is treated with an effective glycosylation amount of all of the compounds listed in FIGS. 4-11.

In a further aspect of the invention, one or more of the compounds listed in FIGS. 4-11 are from Microbulbifer degradans 2-40.

In a further aspect of the invention, one or more of the compounds listed in FIGS. 4-11 essentially comprises a system, or metal ion, chelator, detergent, organic, comprising one or more of the compounds listed in FIGS. 4-11. Ions, inorganic ions, or one or more additional proteins such as biotin and / or albumin.

Some embodiments of the present invention are directed to ethanol prepared by treating lignocellulosic material with at least one effective glycosylation amount of the compounds listed in FIGS. 4-11 to obtain sugars and converting the sugars to produce ethanol. It is about. The conversion and recovery of sugars to ethanol can be carried out by any well-established method known to those skilled in the art, but not limited thereto. For example, ethanologenic microorganisms such as Zymomonas, Erwinia, Klebsiella, Xanthomonas , and Escherichia, preferably Escherichia coli K011 and Klebsiella oxytoca P2 are used.

A further embodiment of the invention comprises contacting a lignocellulosic material with a microorganism expressing an effective glycosylation amount of one or more of the compounds listed in Figures 4-11, preferably the cellulase cel5A listed in Figure 4, to obtain sugars, A method for producing ethanol from lignocellulosic material, comprising converting sugars to produce ethanol. The contact can be carried out in a marine environment, such as in water. One or more of the compounds listed in FIGS. 4-11 may be present in dry form, in a buffer, or in the form of a paste, paint, or micelle. The microorganism may be a recombinant microorganism comprising a chimeric gene comprising at least one polynucleotide encoding a polypeptide comprising a microbulbifer degradans 2-40 or at least one amino acid sequence of a compound listed in FIGS. 4-11; Wherein said gene is operably linked to regulatory sequences that enable expression of amino acid sequences by microorganisms. Recombinant microorganisms can be bacteria or yeasts such as Escherichia coli . In some aspects of the invention, the recombinant microorganism is an ethanologenic microorganism such as Zymomonas, Erwinia, Klebsiella, Xanthomonas , or Escherichia, preferably Escherichia coli K011 or Klebsiella oxytoca P2 species.

Some aspects of the invention are prepared by contacting a lignocellulosic material with a microorganism expressing one or more effective glycosylation amounts of the compounds listed in FIGS. 4-11 to obtain a saccharide and converting the saccharide to produce ethanol. To ethanol.

A further aspect of the present invention is to prepare ethanol from lignocellulosic material, comprising contacting the lignocellulosic material with an ethanologenic microorganism expressing one or more effective glycosylation amounts of the compounds listed in FIGS. 4-11 to produce ethanol. It relates to a manufacturing method. Ethanologenic microorganisms convert one or more effective amounts of the compounds listed in FIGS. 4-11 and subsequent sugars (eg, sugars such as xylose and / or glucose) to ethanol to glycate lignocellulosic material. Express an effective amount of one or more enzymes or enzyme systems that catalyze (either individually or in concert). One or more enzymes or enzyme systems of the ethanologenic organism can be expressed naturally or by any method known to those skilled in the art. For example, the release of one or more enzymes or enzyme systems can be obtained through the use of ultrasound. In some aspects of the invention, ethanologenic microorganisms are transfected to be able to express one or more of the compounds listed in FIGS. 4-11. In some aspects of the invention , the ethanologenic microorganism is a microorganism from Zymomonas, Erwinia, Klebsiella, Xanthomonas , or Escherichia, preferably Escherichia coli K011 or Klebsiella oxytoca P2 species.

A further embodiment of the invention relates to the use for the production of ethanol from one or more lignocellulosic materials of the compounds listed in FIGS. 4-11. Another embodiment relates to the use of microorganisms or ethanologenic microorganisms expressing at least one effective glycosylation amount of the compounds listed in FIGS. 4-11 for preparing ethanol from lignocellulosic material.

While the invention has been described in detail above using specific embodiments, it is to be understood that the description and examples are intended to illustrate structural and functional principles of the invention and are not intended to limit the scope of the invention. On the contrary, the invention is intended to embrace all such alterations, modifications and substitutions within the spirit and scope of the appended claims.

Cited References

                               SEQUENCE LISTING <110> TAYLOR, LARRY EDMUND              WEINER, RONALD M.              HUTCHESON, STEVEN WAYNE              EKBORG, NATHAN A.              HOWARD, MICHAEL   <120> ENZYME SYSTEMS FOR SACCHARIFICATION OF PLANT CELL WALL POLYSACCHARIDES <130> 108172-00124 <140> 11 / 519,104 <141> 2006-09-12 <150> 11 / 121,154 <151> 2005-05-04 <150> 60 / 567,971 <151> 2004-05-04 <160> 214 <170> PatentIn version 3.3 <210> 1 <211> 1167 <212> PRT <213> Microbulbifer degradans <400> 1 Met Thr Ile Lys Arg Trp Pro Phe Asp Arg Lys Gly Pro Pro Lys Lys   1 5 10 15 Pro Asn Ala Lys Lys Leu Leu Ala Ser Leu Ala Ala Ala Leu Ser Leu              20 25 30 Thr Ala Met Gln Ser Thr Ala Ala Val Glu Pro Leu Gln Thr Ser Gly          35 40 45 Asn Gln Ile Leu Val Gly Asn Gln Ala Lys Ala Leu Gly Gly His Ser      50 55 60 Leu Phe Trp His Asn Val Pro Ala Ala Gly Ser Leu Tyr Asn Ala Asp  65 70 75 80 Thr Val Ser Arg Leu Lys Asn Asp Trp Asn Ser Lys Val Ile Arg Ala                  85 90 95 Ala Ile Gly Val Glu Val Pro Phe Asn Ser Glu Asn Thr Tyr Ile Gly             100 105 110 Asn Lys Gly Ser Ser Leu Ala Ala Ile Asp Arg Val Val Asn Ala Ala         115 120 125 Val Ala Asn Asp Met Tyr Val Ile Ile Asp Phe His Thr His His Ala     130 135 140 Asp Gln Val Glu Asn Val Ala His Asp Phe Phe Asn Glu Val Ser Ser 145 150 155 160 Arg Tyr Gly His Leu Asn Asn Val Ile Tyr Glu Val Phe Asn Glu Pro                 165 170 175 Glu Trp Cys Gly Glu His Gly Arg Trp Ala Ser Thr Ile Lys Pro Tyr             180 185 190 Ala Glu Arg Val Ile Gln Thr Ile Arg Asn Asn Asp Pro Asp Asn Leu         195 200 205 Val Ile Val Gly Thr Thr Cys Phe Ser Gln Asp Val Asp Val Ala Ala     210 215 220 Ala Asp Pro Ile Asn Asp Val Asn Val Ala Tyr Thr Leu His Phe Tyr 225 230 235 240 Ala Ala Thr Pro Ala His Gln Gln Pro Leu Arg Asp Lys Ala Gln Thr                 245 250 255 Ala Leu Asp Arg Gly Ala Pro Leu Phe Val Thr Glu Trp Gly Thr Thr             260 265 270 Thr Phe Thr Gly Asp Gly Phe Val Asp Glu Ala Gln Thr Arg Thr Trp         275 280 285 Ile Asn Trp Leu Asn Glu Arg Gly Ile Ser His Val Asn Trp Ser Ala     290 295 300 Ser Thr Gln Pro Glu Ser Ser Ala Ile Trp Asn Gly Asp Met Thr Tyr 305 310 315 320 Lys His Ser Gly Leu Leu Val Gly Glu Leu Val Gln Gln Thr Asn Gly                 325 330 335 Thr Thr Thr Pro Pro Thr Gly Glu Ile Ser Gly Pro Cys Asp Leu His             340 345 350 Phe Val Pro Ala Lys Ala Glu Ala Glu Ser Phe Cys Thr Ala Lys Gly         355 360 365 Ile Gln Phe Glu Thr Thr Thr Asp Thr Gly Gly Gly Gln Asn Met Gly     370 375 380 Trp Leu Asp Ala Gly Asp Trp Val Thr Phe Asp Val Asp Val Pro Ala 385 390 395 400 Ser Gly Gln Tyr Leu Ile Asp Tyr Arg Val Ala Ser Glu Leu Gly Asp                 405 410 415 Gly Arg Phe Arg Thr Glu Ala Ala Asn Gly Thr Ala Leu Gly Thr Ile             420 425 430 Ser Val Pro Asn Thr Gly Gly Trp Gln Asn Trp Gln Thr His Thr His         435 440 445 Thr Val Gln Leu Ser Gln Gly Thr Gln Thr Val Lys Leu Val Ala Glu     450 455 460 Thr Gly Gly Trp Asn Leu Asn Trp Phe Glu Val Arg Ala Gly Glu Val 465 470 475 480 Cys Glu Gly Ala Asp Cys Pro Cys Glu Gly Ala Glu Cys Pro Cys Pro                 485 490 495 Asp Cys Asn Gly Thr Pro Val Lys Phe Glu Ala Glu Thr Phe Val Ala             500 505 510 Met Gln Gly Val Gln Leu Glu Asn Thr Ser Asp Val Gly Gly Gly Gln         515 520 525 Asn Val Gly Tyr Ile Asp Ser Gly Asp Trp Ile Thr Tyr Asn Gly Ala     530 535 540 Leu Pro Ala Ser Ala Asp Asn Arg Tyr Val Val Ser Tyr Arg Val Ala 545 550 555 560 Arg Gln Pro Ser Gly Asn Ala Lys Phe Lys Ile Glu Gln Pro Gly Gly                 565 570 575 Ala Ala Val Tyr Gly Glu Ile Ser Val Pro Ser Thr Gly Gly Trp Gln             580 585 590 Thr Trp Thr Thr Ile Ser His Thr Ile Thr Ile Pro Ala Asn Ala Asn         595 600 605 Gly Phe Ala Leu Ala Ala Ile Asp Gly Gly Trp Asn Ile Asn Trp Ile     610 615 620 Glu Ile Lys Pro Ala Thr Thr Gln Pro Pro Glu Pro Ile Asn Pro Leu 625 630 635 640 Lys Leu Gln Ala Glu Asp Tyr Ile Asn Phe Asn Asp Thr Thr Pro Gly                 645 650 655 Asn Glu Gly Gly Ala His Arg Ser Asp Asp Val Asp Ile Gln Ala Thr             660 665 670 Thr Asp Thr Gly Gly Gly Phe Asn Val Gly Trp Val Asp Ala Gly Glu         675 680 685 Trp Leu Glu Tyr Glu Phe Phe Leu Glu Ser Pro Asp Phe Tyr Ala Ala     690 695 700 Asp Val Arg Val Ala Ser Asp Gln Thr Gly Gly Ala Leu Gln Leu Gln 705 710 715 720 Ile Asp Gly Gln Asn Val Gly Gln Ala Ile Thr Val Gly Asn Thr Gly                 725 730 735 Gly Trp Gln Ala Trp Thr Thr Lys Asn Thr Leu Ile Gly Asp Leu Ser             740 745 750 Ala Gly Thr His Thr Leu Arg Val Tyr Ala Gln Ser Gly Pro Leu Asn         755 760 765 Leu Asn Trp Val Glu Leu Lys Arg Thr Thr Pro Ala Pro Ala Thr Ser     770 775 780 Cys Phe Asn Ile Ala Glu Asp Arg Leu Asn Val His Leu Asp Ala His 785 790 795 800 Cys Thr Ala Gly Ser Asn Leu Gln Tyr Asn Trp Asp Phe Gly Asp Gly                 805 810 815 Asn Ser Ala Thr Gly Val Ala Thr Ser His Ser Tyr Tyr Thr Ser Gly             820 825 830 Thr Tyr Thr Ile Thr Leu Thr Val Ser Asp Thr Arg Thr Thr Asp Thr         835 840 845 Ser Ser Gln Gln Val Thr Val Asp Phe Ser Ala Pro Ala Gly Pro Val     850 855 860 Asp Phe Tyr Gly Glu Leu Met Val Asn Gly Asn Arg Ile His Gly Glu 865 870 875 880 Lys Thr Gly Glu Pro Ala Gln Val Arg Gly Met Ser Phe Phe Trp Ser                 885 890 895 Asn Thr Gly Trp Gly Gln Glu Lys Trp Trp Asn Ala Ser Thr Val Asp             900 905 910 Arg Met Val Asp Glu Phe Lys Val Glu Leu Val Arg Gly Ala Met Gly         915 920 925 Thr Asp Glu Gly Gly Gly Tyr Leu His Asp Ala Ser Asn Lys Ala Arg     930 935 940 Leu Gln Ala Val Val Glu Gln Ala Ile Ala Arg Asn Val Tyr Val Ile 945 950 955 960 Ile Asp Trp His Thr His His Ala Glu Asp Asn Ile Ala Glu Ala Ile                 965 970 975 Thr Phe Phe Ser Glu Met Ala Gln Leu Tyr Gly His His Asp Asn Val             980 985 990 Ile Phe Glu Ile Tyr Asn Glu Pro Leu Asn Thr Thr Ser Ser Trp Gly Thr         995 1000 1005 Ile Lys His Tyr Ala Glu Gln Val Ile Pro Ala Ile Arg Ala His Ser    1010 1015 1020 Asp Asn Leu Ile Val Val Gly Thr Arg Thr Trp Ser Gln Asn Val Asp 1025 1030 1035 1040 Glu Ala Ala Phe Asp Lys Ile Asn Asp Ser Asn Thr Ala Tyr Ala Leu                1045 1050 1055 His Phe Tyr Val Gly Ser His Gly Asn His Val Arg Asn Leu Ala Gln            1060 1065 1070 Thr Ala Leu Asn Asn Gly Ala Ala Ile Phe Ala Ser Glu Trp Gly Ile        1075 1080 1085 Trp Pro Asn Asn Asn Tyr Asp Gly Met Asn Ala Asp Asp Trp Met Asn    1090 1095 1100 Phe Leu Asp Gln Asn Lys Ile Ser Trp Ala Asn Trp Ala Ile Ser Asp 1105 1110 1115 1120 Lys Val Asp Pro Asn Thr Gly Gln Leu Glu Pro Pro Ser Met Phe Asn                1125 1130 1135 Pro Asp Gly Ser Leu Ser Ser Asn Gly Gln Tyr Val Val Asn Lys Leu            1140 1145 1150 Asn Glu Tyr Ala Ala Gln Ala Pro Trp Arg Glu Ala Ile Ala Asn        1155 1160 1165 <210> 2 <211> 3504 <212> DNA <213> Microbulbifer degradans <400> 2 atgacaatta aacgttggcc gttcgaccga aaaggcccac ctaaaaaacc taacgctaaa 60 aaattactcg caagcttagc ggctgcacta agcttaaccg ccatgcaaag cactgcagcg 120 gtagagccat tacaaaccag cggcaatcaa attcttgttg gcaaccaagc caaagccctt 180 ggcggccaca gcttgttttg gcataacgtg ccggcagcag gcagcttata caatgcagat 240 acagtaagca ggcttaagaa tgattggaac tccaaggtta ttcgggccgc aattggggtt 300 gaagtacctt tcaattcaga aaacacctac ataggcaata agggcagctc gctggccgca 360 atagaccgcg tagttaatgc cgctgttgcc aacgatatgt atgtgattat cgattttcat 420 actcaccatg cagatcaagt agaaaacgtt gcccacgact ttttcaacga agtttctagc 480 cgttacggtc atttaaacaa tgttatttat gaagtattta acgagccaga atggtgtggc 540 gagcacggtc ggtgggcatc taccattaag ccctacgccg agcgcgttat ccaaaccatt 600 cgcaacaatg acccagacaa cctagtaata gtaggcacta cctgtttctc gcaagatgta 660 gatgtagccg cagccgaccc cattaacgat gtaaacgtgg cctatacgct acacttttac 720 gcagccaccc ctgcccacca gcaacccttg cgcgacaagg cccaaaccgc gctcgaccgc 780 ggcgcgccac tatttgtaac cgaatggggt acaaccacat ttacaggtga tggttttgta 840 gatgaggcgc aaacgcgcac atggattaac tggttaaacg aacgcggtat tagccacgtt 900 aactggtcgg cgtctaccca gccagaaagc tcagctatat ggaatggcga catgacctac 960 aagcattcgg gcttattggt tggcgaactg gtgcaacaaa caaatggcac aaccacgcca 1020 ccaaccggtg aaataagtgg cccgtgcgat ttacattttg tacctgccaa agccgaggct 1080 gaaagcttct gtaccgccaa aggcattcaa tttgaaacca ccaccgacac gggcggcggc 1140 caaaacatgg gctggctaga tgccggcgac tgggtaactt ttgatgtaga tgtacctgct 1200 agcggccaat atttaataga ttaccgcgta gcatcagagc taggtgatgg tcggttccgc 1260 accgaagccg ccaacggcac tgcccttggc acaatatctg tacccaatac cggcggctgg 1320 cagaattggc aaacgcacac acacacagtg caactctcgc aaggcacaca aaccgttaaa 1380 ctagttgccg aaactggtgg ctggaactta aattggtttg aagtgcgcgc aggtgaggtg 1440 tgcgaaggcg ctgactgccc atgtgaagga gccgaatgcc cttgcccaga ttgcaacggc 1500 acaccggtta agtttgaggc agaaacgttt gtggctatgc aaggcgtgca gctagaaaac 1560 acatccgatg tgggcggcgg ccaaaacgtt ggctacattg atagcggcga ctggataact 1620 tacaacgggg ccttgcccgc aagtgcagac aaccgctatg tagtgtctta tagagtagcg 1680 cgtcaaccta gcggcaatgc caaatttaaa atagaacagc caggtggagc agcggtatat 1740 ggcgaaattt cggtgcccag caccggcggc tggcaaacat ggacaaccat tagccacacc 1800 ataacaattc ccgctaacgc aaacggcttt gcactagcag caatagatgg cggttggaat 1860 ataaactgga tagaaataaa accggcgacc actcaaccac ccgagccaat caacccgtta 1920 aaacttcaag ctgaagatta catcaacttt aacgacacca cccccggtaa cgaaggcggt 1980 gcacacagaa gcgatgatgt agatattcaa gcaactaccg ataccggtgg cggttttaat 2040 gttggctggg tagacgctgg cgaatggcta gagtatgagt tctttttaga gtctcctgat 2100 ttttatgcag ctgatgtacg ggttgcttca gaccaaactg gcggcgcact gcaactacaa 2160 atagatggcc aaaacgttgg ccaagccatt accgttggca acaccggtgg ctggcaagcg 2220 tggacaacca aaaacacact cattggcgac ctaagtgcag gcacccacac gttgcgtgta 2280 tacgcgcaaa gcggcccatt aaatttaaac tgggtagagc taaagcgtac aacgcccgca 2340 ccagccactt cgtgttttaa tattgccgaa gaccgcttaa acgttcacct agatgcgcac 2400 tgtactgcag gcagcaacct gcaatacaat tgggattttg gtgacggcaa cagcgcaacc 2460 ggcgtagcca ctagccacag ctactacact agcggcactt acaccattac cttaaccgtt 2520 agtgataccc gcaccacaga cacctctagc caacaggtaa cggtagattt ttctgcccct 2580 gcaggccctg tggattttta cggcgaacta atggtgaatg gcaaccgcat tcacggcgaa 2640 aaaaccggcg aacccgcaca agtacgcggc atgagctttt tttggagcaa caccggttgg 2700 ggccaagaaa aatggtggaa cgccagcacc gtggaccgca tggttgatga gttcaaagta 2760 gaacttgtgc gcggcgcaat gggcactgat gaaggcggcg gttatttaca cgacgcgtct 2820 aataaggctc gcttacaagc agttgttgaa caagccattg cacgcaatgt gtatgtaatt 2880 atcgactggc acacccacca tgccgaagat aacattgccg aagccattac attctttagc 2940 gaaatggcgc agctttatgg ccaccacgac aacgtgattt tcgagattta caacgagcca 3000 ttaaacacca caagctgggg cactattaag cactacgctg aacaagttat tcctgctatt 3060 cgcgctcatt ccgataattt aattgttgtg ggcacgcgca cctggtcgca aaacgtagac 3120 gaagccgcgt tcgataaaat taacgacagc aacaccgcct acgccctgca cttttatgtt 3180 ggctcgcacg gcaaccacgt tcgcaaccta gcacaaaccg cactaaacaa cggcgcggct 3240 atttttgcta gcgaatgggg aatttggcca aacaacaact acgatggcat gaacgccgac 3300 gattggatga actttttaga ccaaaacaaa atatcttggg ctaactgggc catatccgac 3360 aaagtagacc ccaacacagg ccaactagaa ccacccagca tgttcaaccc agacggcagc 3420 ctaagcagta atggtcaata tgtagtgaac aaactaaatg aatacgcagc acaagcaccg 3480 tggagggagg caatcgctaa ttga 3504 <210> 3 <211> 133 <212> PRT <213> Microbulbifer degradans <400> 3 Met Val Val Ser Leu Ala Asp Asn Ser Ala Gly Ala Ile Ser Cys Trp 1 5 10 15 His Ala Lys Ala Ser Pro Pro Glu Glu Leu Glu Glu Leu Leu Asp Glu             20 25 30 Glu Leu Glu Leu Asp Glu Leu Glu Leu Glu Glu Glu Leu Glu Glu Leu         35 40 45 Val Glu Glu Leu Leu Glu Glu Leu Leu Asp Glu Leu Leu Leu Asp Glu     50 55 60 Leu Glu Asp Glu Pro Leu Ala Ala Pro Pro Ser Leu Pro Pro Pro Gln 65 70 75 80 Ala Val Ser Pro Ala Lys Gln Leu Ile Ser Ser Ala Asp Phe Lys Lys                 85 90 95 Val Ser Phe Arg Val Gln Leu Asn Ala Val Arg Val Lys Ser Lys Arg             100 105 110 Asn Ile Asn His Ser Arg Ile Phe Leu Phe Trp Leu Phe Ser His Phe         115 120 125 Arg Ser Arg Arg Cys     130 <210> 4 <211> 566 <212> PRT <213> Microbulbifer degradans <400> 4 Met Phe Leu Leu Asp Phe Thr Arg Thr Ala Phe Ser Cys Thr Arg Lys 1 5 10 15 Leu Thr Phe Leu Lys Ser Ala Leu Leu Ile Ser Cys Phe Ala Gly Leu             20 25 30 Thr Ala Cys Gly Gly Gly Ser Asp Gly Gly Ala Ala Ser Gly Ser Ser         35 40 45 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser     50 55 60 Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 65 70 75 80 Ser Ser Ser Ser Asn Ser Ser Ser Ser Ser Ser Gly Gly Asp Ala Leu                 85 90 95 Ala Cys Gln His Glu Met Ala Pro Ala Leu Leu Ser Ala Ser Asp Thr             100 105 110 Thr Met Val Gln Ala Glu Tyr Tyr Asp Thr Cys Ala Ser Ser Ala Leu         115 120 125 Asp Asn Thr Thr Gly Asn Ser Gly Gly Glu Leu Arg Thr Asp Asp Val     130 135 140 Asp Ile Val Ala Ile Ala Asp Gly Tyr Ala Ile Thr Asp Met Gln Ser 145 150 155 160 Gly Glu Tyr Val Glu Tyr Ser Leu Thr Val Gln Thr Ser Gly Leu Phe                 165 170 175 Asp Ile Ser Phe Ala Val Gln Pro His Ala Ala Asn Thr Ala Gly Leu             180 185 190 Ala Leu Ser Val Asp Gly Ala Val Leu Gly Thr Val Asp Ile Ala Ala         195 200 205 Asn Asp Ser Thr Ala Phe Gly Glu Tyr Thr Leu Asn Gly Val Tyr Ile     210 215 220 Ser Asp Gly Ala Gln Val Ile Arg Val Thr Met Ala Gly Glu Gly Ala 225 230 235 240 Ala Ile Gly Leu Asp Ser Ile Ala Phe Asn Tyr Thr Asp Asn Thr Val                 245 250 255 Tyr Thr Pro Glu Asn Ala Val Leu Gly Met Gly Ile Gly Ile Asn Leu             260 265 270 Gly Asn Thr Leu Asp Ala Phe Pro Asn Glu Gly Asp Trp Ala Pro Ala         275 280 285 Ala Gln Glu Tyr Tyr Phe Lys Ala Tyr Lys Asp Ala Gly Phe Arg His     290 295 300 Val Arg Ile Pro Ala Thr Trp Asp Asp His Thr Ala Asp Thr Ala Pro 305 310 315 320 Tyr Ala Val Asn Ala Ala Arg Met Asp Arg Thr Glu Gln Ile Val Asp                 325 330 335 Trp Ala Leu Ala Gln Gly Tyr Phe Val Ile Leu Asn Ala His His Glu             340 345 350 His Trp Leu Lys Glu Asn Tyr Gly Asn Gln Thr Tyr Arg Asp Arg Phe         355 360 365 Asp Ala Ile Trp Gln Gln Ile Ala Glu Arg Phe Lys Asn Lys Ser Ala     370 375 380 Arg Leu Met Phe Glu Ile Leu Asn Glu Pro Asn Gly Met Thr Val Ala 385 390 395 400 Asp Val Asp Asp Leu Asn Pro Arg Ile Leu Asp Ile Ile Arg Glu Thr                 405 410 415 Asn Pro Thr Arg Leu Val Val Phe Ser Gly Asn Gly Tyr Thr Pro Val             420 425 430 Asp Ala Leu Leu Ala Ala Ala Ile Pro Asn Asp Asp Tyr Leu Ile Gly         435 440 445 Asn Phe His Ser Tyr Asp Pro Trp Gln Phe Gly Gly Gln Cys Val Arg     450 455 460 Ser Trp Gly Thr Glu Gln Asp Tyr Thr Asp Leu Glu Asn Ile Tyr Lys 465 470 475 480 Arg Ala Asn Thr Trp Ser Glu Gln His Asp Ile Pro Val Met Val Asn                 485 490 495 Glu Phe Gly Ala Ala His Tyr Asp Phe Thr Ala Pro Gln Asn Val Cys             500 505 510 Asn Gln Gln Ala Arg Leu Ala Tyr Leu Gly Ala His Ala Thr Phe Ala         515 520 525 Ile Gln Tyr Gly Phe Gly Ala Ser Val Trp Asp Asp Gly Gly Ser Phe     530 535 540 Glu Val Tyr Lys Arg Gly Glu Asn Ser Trp Arg Glu Ala Lys Asp Val 545 550 555 560 Leu Val Ala Pro Asn Pro                 565 <210> 5 <211> 1701 <212> DNA <213> Microbulbifer degradans <400> 5 atgtttcttt tagactttac ccgcactgcg tttagctgta cacgaaagct tacctttttg 60 aaatccgcgc tacttataag ctgctttgcc gggcttactg cctgtggtgg cgggagtgat 120 ggcggtgctg caagtggctc atcctctagc tcgtctagca gcagttcgtc tagtagctct 180 tcgagcagtt cttcaactag ttcctcaagc tcctcttcaa gctctagttc gtccagttcc 240 agctcttcgt ctaatagttc ctctagctcc tctggtggcg atgctttagc gtgccagcat 300 gaaatggcac cagcgctatt atctgcaagt gatactacca tggtgcaagc ggagtattac 360 gatacctgtg cttcttcggc attagataac accactggta acagtggcgg tgagttgcga 420 actgacgatg tagatatagt ggccattgcg gacggctatg ctattacgga tatgcagtca 480 ggcgagtacg tagaatattc actaacagtg caaacttccg gtttgtttga cattagtttt 540 gcggtacagc cgcacgcagc taatactgcc ggtttggcgc tgagtgtaga tggcgcagtg 600 ttaggcacag ttgatattgc cgctaatgac agcaccgcat ttggcgaata tacgcttaac 660 ggcgtgtaca taagcgatgg cgcgcaagta ataagggtaa ccatggccgg cgaaggcgct 720 gctattgggt tagattccat tgcctttaat tacaccgata ataccgttta caccccagaa 780 aacgccgtgt tgggtatggg aataggtatt aacctaggca ataccttaga tgccttcccc 840 aacgaaggtg actgggcacc ggctgcgcag gaatactatt ttaaagccta caaggatgca 900 ggtttccgcc atgtacgcat cccagcaact tgggatgatc acacggctga tacagccccc 960 tacgctgtaa atgcagcacg tatggatcgc actgagcaga ttgtagattg ggccttggcg 1020 cagggctatt tcgtaattct taatgcccac cacgaacact ggctaaaaga aaactacggc 1080 aatcaaacat accgcgatcg ctttgatgca atttggcagc aaattgccga acgctttaag 1140 aataagtcgg ctcgcttaat gtttgagata ctcaatgagc caaacggcat gacagtggcc 1200 gatgtggatg acctcaaccc acgtattctc gatattattc gcgaaaccaa tcccacgcga 1260 ttggtagtgt tctctggtaa tgggtatacc cctgtggatg ccttacttgc ggctgcaatc 1320 cctaatgatg attaccttat tggtaacttt cactcctacg acccttggca gtttggcggt 1380 cagtgcgtac gatcgtgggg tacagagcaa gattacaccg acctagagaa catatataag 1440 cgcgcaaata cttggtctga gcagcacgac atacccgtta tggtgaacga atttggcgct 1500 gcccattacg attttactgc accgcagaat gtatgtaacc agcaggctcg tttggcttat 1560 ttaggtgccc atgccacatt tgctattcag tacggctttg gcgcaagtgt atgggacgac 1620 ggtggatcat ttgaggtgta caagcgcggt gaaaatagct ggcgcgaagc taaagatgta 1680 ttagtggcgc caaacccgta g 1701 <210> 6 <211> 451 <212> PRT <213> Microbulbifer degradans <400> 6 Met Arg Ile Ile Thr Ala Phe Ala Val Met Leu Leu Cys Ile Thr Gly 1 5 10 15 Cys Ser Gly Ser Gly Ala Ser Asp Ser Pro Gln Ala Ser Asn Ser Ser             20 25 30 Ser Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser         35 40 45 Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser     50 55 60 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly Gly Glu Ala Leu 65 70 75 80 Tyr Pro Ser Tyr Asn Thr Asn Pro Pro Ala Pro Asp Met Thr Gly Met                 85 90 95 Thr Ser Thr Ala Thr Gln Leu Ala Asp Arg Ile Thr Val Gly Trp Asn             100 105 110 Ile Gly Asn Thr Leu Glu Ala Ile Gly Gly Glu Thr Asn Trp Gly Asn         115 120 125 Pro Leu Val Thr Asn Glu Leu Ile Gln Ala Val Lys Ala Ser Gly Phe     130 135 140 Asp Ser Ile Arg Ile Pro Ala Ala Trp Asp Gln Tyr Ala Asn Gln Glu 145 150 155 160 Thr Ala Ala Ile Asp Ile Asn Trp Leu Asn Arg Val Lys Gln Val Val                 165 170 175 Gln Tyr Ser Ile Asp Asn Asp Met Val Val Val Leu Asn Ile His Trp             180 185 190 Asp Gly Gly Trp Leu Glu Arg Asn Val Glu Pro Ser Glu Gln Val Ala         195 200 205 Val Asn Ala Lys Gln Lys Ala Tyr Trp Glu Gln Ile Ala Thr His Leu     210 215 220 Arg Asp Phe Asp Glu Arg Leu Ile Phe Ala Ser Ala Asn Glu Pro His 225 230 235 240 Val Glu Thr Glu Ala Gln Met Ala Val Leu Asn Val Tyr His Gln Thr                 245 250 255 Phe Val Asp Thr Val Arg Ala Thr Gly Gly Lys Asn Ala Tyr Arg Val             260 265 270 Leu Val Leu Gln Gly Pro Lys Thr Asp Ile Glu Thr Thr Ser Leu Leu         275 280 285 Trp Thr Gln Met Pro Gln Asp Ser Ala Val Asn Lys Leu Met Ala Glu     290 295 300 Leu His Phe Tyr Thr Pro Tyr Asn Phe Thr Leu Met Asn Val Asp Glu 305 310 315 320 Ser Trp Gly Asn Gln Phe Tyr Tyr Trp Gly Glu Gly Asn His Ser Thr                 325 330 335 Thr Asp Thr Gly Arg Asn Pro Thr Trp Gly Glu Glu Ala Thr Val Asp             340 345 350 Ser Leu Leu Ala Ile Thr Lys Gln Gln Phe Val Asp Gln Gly Ile Pro         355 360 365 Val Ile Ile Gly Glu Tyr Gly Ala Gln Arg Arg Asp Asn Leu Thr Gly     370 375 380 Asp Glu Leu Ala Leu His Leu Gln Ser Arg Asn Tyr Tyr Leu Lys Tyr 385 390 395 400 Val Thr Gln Lys Cys Val Glu Leu Gly Leu Lys Pro Phe Tyr Trp Asp                 405 410 415 Thr Gly Gly Leu Asp Asn Asn Gln Ser Gly Leu Phe Asn Arg Ser Thr             420 425 430 Tyr Gln Val Phe Asp Gln Asn Ala Leu Asp Ala Ile Met Glu Gly Ala         435 440 445 Arg Gly Glu     450 <210> 7 <211> 1356 <212> DNA <213> Microbulbifer degradans <400> 7 atgagaataa taacggcgtt tgcagttatg ctgctatgca taacaggctg tagcggatcg 60 ggcgcgagtg atagcccgca agcatccaat tcgtcttcgg gcagttcttc tagctctagc 120 agttcgtcaa gttcgagcag ttcctctagt tcgtcgtcta gctcttcaac aagctctagc 180 agctcatcta gctccagctc atcatcaagc tctagcagtt cttcgggcgg cgaagcgctt 240 tacccaagct acaatacaaa cccgccagcg ccagatatga ccggcatgac aagtactgcc 300 acacaactag cagatcgtat aaccgtgggc tggaatattg gtaacacgct agaggcaata 360 ggcggcgaaa ccaactgggg taacccgctg gttactaacg aattaattca agcggtaaaa 420 gccagtggct ttgattccat tcgtataccc gccgcgtggg atcaatacgc caaccaagaa 480 acggccgcaa tagatataaa ctggctaaac cgcgttaaac aagttgtgca atacagcata 540 gataacgaca tggtggtagt gctaaacatc cactgggatg gcggttggct agagcgcaat 600 gtagagccca gcgagcaagt agcagtaaat gcaaaacaaa aagcctattg ggaacaaatt 660 gccactcacc tgcgcgactt tgacgagcgc ctaatatttg ccagcgccaa cgaaccccat 720 gtagaaaccg aagcacaaat ggccgtacta aacgtatacc atcaaacgtt tgtagataca 780 gtgcgtgcaa ctggcggtaa aaatgcttac cgcgtactgg tattgcaggg gccaaaaaca 840 gatatagaaa ccacctcgct attgtggacc caaatgccgc aagatagcgc cgtaaataaa 900 cttatggcag agctacactt ctataccccg tacaacttta cgttaatgaa tgtagatgaa 960 agctggggca accagttcta ctactggggc gaaggtaatc attccactac cgacacaggc 1020 cgcaacccaa cctggggcga agaagcaaca gtagattcac tgctggcaat taccaaacaa 1080 cagtttgtgg accaaggtat acccgtaatt attggcgaat acggtgcaca acgccgcgat 1140 aaccttaccg gcgatgaatt ggccctgcac ttacaatcgc gcaactacta cttaaaatac 1200 gttactcaaa aatgtgtaga gctaggctta aaaccttttt attgggatac cggcggctta 1260 gacaacaatc aatctggcct gtttaatcgc agtacctacc aagtatttga tcaaaatgcc 1320 ctagatgcca ttatggaagg ggccagaggg gaataa 1356 <210> 8 <211> 621 <212> PRT <213> Microbulbifer degradans <400> 8 Met Leu Lys His Gln Phe Ser Lys Ala Leu Arg Ala Leu Gly Phe Gly 1 5 10 15 Gly Ala Val Phe Ala Ala Ser Leu Met Ala Ser Gln Ala Ser Ala Leu             20 25 30 Glu Cys Glu His Ser Ile Ser Asn Asp Trp Gly Ala Gly Phe Thr Gly         35 40 45 Ala Met Lys Val Thr Asn Asn Asp Ser Ser Pro Ile Thr Gly Trp Arg     50 55 60 Val Glu Trp Ala Tyr Ser Gly Asn Val Asn Ile Val Asn Ser Trp Asn 65 70 75 80 Ala Ser Val Thr Lys Gly Ser Asn Tyr Val Ala Val Asp Ala Gly Trp                 85 90 95 Asn Gly Asn Leu Gln Pro Ser Gln Ser Thr Glu Phe Gly Leu Gln Gly             100 105 110 Asp Gly Ala Asp Arg Asn Val Thr Ile Ile Ser Cys Val Ala Glu Gly         115 120 125 Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser     130 135 140 Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Thr 145 150 155 160 Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Thr Ser Ser                 165 170 175 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly Gly Asn Cys Val             180 185 190 Ala Met Cys Asn Trp Tyr Gly Glu Asn Arg Pro Val Cys Ala Asn Gln         195 200 205 Asn Thr Gly Trp Gly Trp Glu Asn Asn Gln Ser Cys Ile Gly Ala Asn     210 215 220 Thr Cys Asn Asp Gln Trp Gly Asp Gly Gly Val Val Ser Ser Cys Gly 225 230 235 240 Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser                 245 250 255 Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser Ser             260 265 270 Ser Ser Ser Ser Ser Gly Gly Leu Ser Ala Val Glu Phe Ser Gln Gln         275 280 285 Met Gly Leu Gly Trp Asn Leu Gly Asn Ser Leu Glu Ala Ile Gly Gly     290 295 300 Glu Thr Ala Trp Gly Asn Pro Met Val Thr Gln Gln Leu Ile Asn Ser 305 310 315 320 Ile Lys Ala Ala Gly Phe Asp Thr Ile Arg Ile Pro Val Ala Trp Ser                 325 330 335 Gln Phe Ser Asp Glu Ala Asn Phe Val Ile Asn Ser Asn Trp Ile Ala             340 345 350 Arg Val Glu Glu Val Val Asn Tyr Ala Leu Ser Ala Asp Met Tyr Val         355 360 365 Val Met Asn Gln His Trp Asp Gly Gly Trp Met Gln Pro Thr Tyr Ala     370 375 380 Gln Gln Glu Tyr Val Asn Asn Arg Leu Gln Ile Met Trp Thr Gln Ile 385 390 395 400 Ala Asn His Phe Lys Asp Tyr Asp Ser Arg Leu Leu Phe Ala Gly Thr                 405 410 415 Asn Glu Val Met Val Glu Gly Asp Tyr Gly Thr Pro Thr Phe Glu Tyr             420 425 430 Tyr Thr Val Gln Asn Ser Phe Asn Gln Thr Phe Val Asp Ala Val Arg         435 440 445 Ala Thr Gly Gly Ala Asn Ala Ser Arg Tyr Leu Val Val Gln Gly Phe     450 455 460 Asn Thr Asn Ile Asp His Thr Val Asn Phe Ala Val Val Pro Thr Asp 465 470 475 480 Pro Ala Thr Asn Arg Leu Met Met Glu Val His Tyr Tyr Asp Pro Tyr                 485 490 495 Asn Phe Thr Leu Asn Thr Asn Ser Asn Ile Thr Gln Trp Gly Val Ile             500 505 510 Ala Thr Asp Pro Ser Val Thr Glu Thr Trp Ala Asn Glu Ser Tyr Val         515 520 525 Asp Ala Thr Phe Gln Lys Met Lys Thr Asn Phe Val Asp Gln Gly Ile     530 535 540 Ala Val Ile Leu Gly Glu Tyr Gly Val Val Ser Arg Ala Asn Val Ala 545 550 555 560 Gly His Glu Thr Tyr Arg Glu Tyr Trp Asn Gln Tyr Ile Thr Gln Ser                 565 570 575 Ala Val Asp His Gly Met Val Pro Ile Tyr Trp Asp Asn Gly Tyr Ser             580 585 590 Gly Asp Gly Gly Met Ala Leu Phe Asp Arg Ala Ser Gly Asn Gln Leu         595 600 605 Tyr Pro Asn Ile Ile Asn Ala Ile Ile Asn Ala Gly Asn     610 615 620 <210> 9 <211> 85 <212> PRT <213> Microbulbifer degradans <400> 9 Met Leu Glu Glu Glu Leu Glu Val Glu Leu Glu Glu Glu Leu Val Glu 1 5 10 15 Glu Leu Glu Leu Leu Asp Glu Glu Val Leu Glu Leu Asp Glu Glu Leu             20 25 30 Leu Glu Glu Leu Glu Glu Leu Asp Glu Leu Glu Asp Asp Pro Pro Ser         35 40 45 Ala Thr Gln Leu Ile Met Val Thr Phe Leu Ser Ala Pro Ser Pro Cys     50 55 60 Lys Pro Asn Ser Val Asp Trp Leu Gly Cys Lys Leu Pro Phe His Pro 65 70 75 80 Ala Ser Thr Ala Thr                 85 <210> 10 <211> 1866 <212> DNA <213> Microbulbifer degradans <400> 10 atgttgaaac atcaattcag caaagcgctg cgtgcgctag gctttggtgg ggctgtgttt 60 gcggcatcgc taatggctag ccaagcaagt gcccttgagt gtgagcattc aatcagtaat 120 gattggggcg ccggctttac cggtgcaatg aaagttacca ataatgactc tagccccatt 180 accggttggc gggtcgaatg ggcgtatagc ggcaatgtaa atattgttaa ttcgtggaac 240 gcctcagtaa caaaaggcag taattatgtt gccgtagatg ccggatggaa tggtaattta 300 cagccgagcc aatctaccga atttggctta cagggtgatg gcgccgatag aaatgtaacc 360 attattagtt gtgttgccga aggcggatca tcttctagtt catcaagttc ttccagctcc 420 tcaagtagtt cttcatctag ctcaagtact tcttcatcga gtagctcaag ttcctcgacg 480 agctcttctt ctagttcgac ttctagctct tcttcaagca cctcttctag ctcatcgtcc 540 agttcatcaa gctcttcttc gggcggcaac tgtgttgcaa tgtgtaattg gtacggtgaa 600 aaccgccctg tttgtgccaa tcaaaatact ggttgggggt gggaaaacaa ccaaagctgt 660 ataggtgcaa acacctgtaa cgatcaatgg ggcgacgggg gcgtggtgtc cagctgtggt 720 acgtctagct cttcatccag ttcttcgtcc agttcgtcta ccagttcatc ctcgtcttct 780 agctcgagca ccagctctac aagcagctca tcaagctcta gttcgtcgtc tggtgggtta 840 agcgcggtag agttttcgca gcaaatgggc ttggggtgga atcttggaaa ctccctagaa 900 gcgattggtg gcgaaaccgc gtggggcaac ccaatggtta cgcagcaatt aattaactcc 960 ataaaagctg ctgggttcga cactattcgc attccggttg cgtggagcca attctcggac 1020 gaagctaatt ttgttatcaa tagcaattgg attgcacgcg tagaagaagt agtgaactac 1080 gcattgagcg ccgatatgta cgtggtaatg aaccaacatt gggacggcgg ttggatgcag 1140 cccacatatg cacagcaaga atatgttaac aatcgcttgc aaattatgtg gacgcaaata 1200 gctaatcact ttaaagatta cgatagtcgc ttactgtttg caggcaccaa cgaagtgatg 1260 gtggaaggcg attacggtac gcccaccttc gaatactaca cagtacaaaa tagctttaac 1320 caaacgtttg tggatgctgt acgtgcaacc ggtggcgcta atgctagccg ttacttagtg 1380 gtacaggggt ttaataccaa catagatcac acggtgaact tcgcggtagt gccaaccgac 1440 ccggcaacaa acaggttaat gatggaagta cactattacg acccctataa ctttacgtta 1500 aataccaaca gcaacattac tcagtggggc gtaattgcaa ctgaccctag cgttaccgaa 1560 acatgggcga atgaatctta tgtggatgcg actttccaaa aaatgaaaac taacttcgtt 1620 gatcaaggta tagcggtaat tttaggtgag tacggggttg tatcgcgcgc gaatgtggcc 1680 gggcacgaaa cttaccgaga gtattggaac caatacatta ctcaatctgc ggtagatcat 1740 ggaatggtgc ctatttattg ggataacggt tattccggtg atggtggtat ggcattgttt 1800 gatcgcgcca gtggcaatca actttacccc aatattatta acgcaattat caatgccggt 1860 aactaa 1866 <210> 11 <211> 673 <212> PRT <213> Microbulbifer degradans <400> 11 Met Leu Ile Gly Thr Val Thr Ala Ser Ala Leu Val Gly Arg Gly Arg 1 5 10 15 Gly Thr Pro Lys Lys Ile Ile Asn Lys Gly Ser Ile Met Trp Gln Ile             20 25 30 Asn Lys Ser Ala Leu Ala Ala Val Val Leu Val Cys Ser Ser Ser Ser         35 40 45 Phe Ala Gln Ser Ala Cys Asp Thr Gln Arg Ile Glu Ala Glu Asn Tyr     50 55 60 Val Ala Met Ser Gly Ile Gln Thr Glu Ser Thr Ala Asp Thr Gly Gly 65 70 75 80 Gly Leu Asn Val Gly Trp Ile Asp Ala Gly Asp Trp Leu Ser Tyr Gln                 85 90 95 Val Asn Leu Pro Ala Ala Gly Gln Tyr Glu Val Arg Tyr Arg Val Ala             100 105 110 Ser Arg Asn Gly Gly Gly Val Leu Arg Leu Glu Gly Asn Ala Gly Gln         115 120 125 Thr Leu Tyr Gly Thr Met Asn Val Pro Asn Thr Gly Gly Trp Gln Asn     130 135 140 Trp Gln Thr Leu Ser His Ser Val Thr Leu Ala Ala Gly Glu Gln Ser 145 150 155 160 Ile Gly Ile Gly Val Pro Ser Gly Gly Phe Asn Ile Asn Trp Leu Glu                 165 170 175 Phe Val Pro Leu Asp Cys Ser Gly Pro Ile Asp Pro Pro Ile Asn Pro             180 185 190 Pro Ser Asn Cys Ala Ser Ile Val Phe Glu Ala Glu Asn Tyr Asp Gln         195 200 205 Met Ser Gly Ile Arg Thr Gln Thr Thr Ser Asp Thr Gly Gly Gly Leu     210 215 220 Asn Val Gly Trp Ile Asp Ala Gly Asp Trp Leu Ser Tyr Ala Thr Val 225 230 235 240 Asn Ile Pro Ser Thr Gln Val Tyr Asn Phe Glu Tyr Arg Val Ala Ser                 245 250 255 Pro Asn Gly Gly Ser Phe Asn Leu Gln Gly Ser Ala Gly Ala Glu Asn             260 265 270 Phe Asp Thr Ala Thr Leu Pro Asn Thr Gly Gly Trp Gln Asn Trp Thr         275 280 285 Thr Val Thr Gly Ser Ala Leu Leu Pro Ala Gly Asn Val Asn Phe Gly     290 295 300 Ile Ser Ala Ile Thr Gly Gly Trp Asn Ile Asn Trp Phe Lys Ala Thr 305 310 315 320 Pro Glu Ser Cys Asp Asp Ile Asn Pro Pro Ser Thr Gly Ile Thr Ala                 325 330 335 Lys Gln Ala Ala Ala Ala Met Gly Lys Gly Phe Asn Leu Gly Gln Met             340 345 350 Phe Glu Ser Thr Gln His Pro Arg Thr Phe Asn Ala Ala Lys Ser Lys         355 360 365 Ile Asp Ala Tyr Tyr Asn Met Gly Tyr Arg Asn Val Arg Ile Pro Ile     370 375 380 Thr Trp Thr Glu Ala Val Gly Gly Asn Arg Leu Val Ala Asp Ala Asn 385 390 395 400 Val Gly Ala Val Asn Arg Asn His Ser Arg Leu Ala Val Ile Thr Gln                 405 410 415 Val Val Asp Tyr Ala Leu Ser Leu Pro Gly Met Tyr Val Val Ile Asn             420 425 430 Ala His His Glu Gly Gly Leu Lys Thr Asn Asn Arg Trp Trp Val Leu         435 440 445 Glu Thr Leu Trp Ala Asp Ile Ala Asp Ile Phe Lys Asp Arg Asp His     450 455 460 Arg Leu Leu Phe Glu Ile Leu Asn Glu Pro His Leu Ser Asp Ala Asn 465 470 475 480 Lys Ser Pro Met Pro Pro Ala Asn Leu Arg Phe Met Thr Gly Lys Ala                 485 490 495 Tyr Asn Lys Ile Arg Ala Ile Asp Ala Gln Arg Ile Val Ile Ile Gly             500 505 510 Gly Asn Gln Trp Phe Gly Ala Gly Glu Met Ala Asn Val Trp Pro Asn         515 520 525 Leu Asn Asp Val Gly Gly Gly Ser Asp Ala Tyr Val Met Ala Thr Phe     530 535 540 His His Tyr Asp Pro Trp Ser Phe Ser Gly Asp Asn Gln Gly Asp Tyr 545 550 555 560 Ala Asp Ala Trp Thr Leu Ser Asn Val Gly Asn Pro Met Asp Ile Met                 565 570 575 Gln Ser Trp Ala Asn Gly Val Gly Gln Gly Met Pro Val Tyr Ile Gly             580 585 590 Glu Trp Gly Val Gly Trp Gly Ser Arg Tyr Ser Ala Met Gln Cys Asn         595 600 605 Asn Ile Arg Tyr Trp Tyr Gln Leu Phe Asp Ala Ser Tyr Ala Ser Ala     610 615 620 Lys Gly Gln Pro Thr Ala Val Trp Asp Asp Gly Gly Trp Phe Lys Ile 625 630 635 640 Phe Asp His Gly Thr Asn Ser Phe Asn Asn Asn Leu Ala Gln Cys Ile                 645 650 655 Gly Gly Asn Cys Ala Trp Asp Gly Ala Asp Arg Phe Asn Ser Gly Cys             660 665 670 Asn      <210> 12 <211> 2022 <212> DNA <213> Microbulbifer degradans <400> 12 atgttgattg gtactgttac ggcttcagca ctggttggtc gaggccgtgg cacccctaaa 60 aaaataatca acaagggttc tattatgtgg caaatcaaca aatcggcttt agcggccgtg 120 gtattagtgt gttcctcatc tagctttgcg caatctgcat gtgacactca acgcattgaa 180 gccgaaaatt acgtggcaat gagtggtatt caaaccgaaa gcacggcaga cactggtggc 240 ggtttaaatg tgggctggat agacgccggc gactggctta gttaccaagt taacctacct 300 gctgcagggc agtacgaggt gcgctatcgc gttgccagta gaaatggcgg cggtgtactt 360 cggttagagg gcaatgccgg tcaaaccttg tatggaacta tgaatgtacc caacacgggt 420 ggctggcaaa attggcaaac cctttctcat tcagtgacat tagcggcagg agagcagtct 480 attggtattg gtgtgccaag cggcgggttt aatattaatt ggctggagtt cgtaccttta 540 gattgcagtg ggccaatcga cccgcccatt aacccacctt cgaactgcgc gagcattgta 600 ttcgaggccg aaaattacga tcaaatgagc ggcattagaa cgcaaaccac aagtgatacc 660 ggaggcggct taaatgtggg gtggatagat gctggcgact ggcttagcta tgccactgtg 720 aatatcccca gcacgcaggt gtacaatttt gaataccgtg tggctagccc taatggcggc 780 agttttaatt tgcagggttc ggctggcgca gagaattttg ataccgctac tttgcccaat 840 acgggtggtt ggcaaaattg gacaacggta acaggctcgg cgcttttacc tgctggcaat 900 gtgaatttcg gtattagtgc gattactggt ggctggaata taaactggtt taaagctaca 960 ccagagagct gtgatgatat aaaccctcca agtaccggta ttactgctaa gcaagcagcg 1020 gcagccatgg gcaaggggtt taatttgggg caaatgttcg aaagtacgca acacccaaga 1080 acatttaatg ctgcaaaaag taaaatagat gcttactaca atatgggcta cagaaatgtg 1140 cgcatcccta ttacttggac tgaagccgta ggcggaaaca ggcttgttgc agatgcaaat 1200 gtaggcgcag tcaatcgcaa ccactctcgc ttagctgtaa ttactcaagt agtagattac 1260 gcgctttcgc tacccggcat gtacgtggtt attaatgcgc atcacgaagg tggattaaaa 1320 accaataatc gctggtgggt gttagaaact ctgtgggcag atattgccga tatatttaaa 1380 gacagagatc accgtttgct atttgaaata ttaaacgagc cacacctaag cgatgccaat 1440 aagtcgccta tgccccccgc caatttgcgt tttatgacgg gcaaagccta taacaaaatt 1500 cgcgcgatag atgcgcagcg aatcgttatt attggtggca accagtggtt tggtgcaggt 1560 gaaatggcaa acgtatggcc aaaccttaat gatgttggcg gcggttccga tgcatatgta 1620 atggctactt ttcaccatta cgacccgtgg tcgtttagtg gcgataacca aggcgattac 1680 gccgatgctt ggacgctatc taacgtgggt aacccaatgg atataatgca aagctgggca 1740 aacggcgtag gccaaggtat gcctgtgtat attggcgagt ggggcgtagg ttggggcagc 1800 cgctacagcg ccatgcagtg caataatatt cgctattggt accagctgtt cgacgcgagc 1860 tatgcctcgg caaaaggcca gcctacggca gtgtgggatg acggcggttg gtttaaaata 1920 ttcgaccacg gtaccaacag cttcaataat aatttagccc aatgtattgg tggaaactgc 1980 gcttgggatg gcgccgatag atttaattct ggctgtaatt aa 2022 <210> 13 <211> 365 <212> PRT <213> Microbulbifer degradans <400> 13 Met Arg Thr Thr Lys Phe Leu Ala Leu Ala Leu Cys Leu Leu Ala Ser 1 5 10 15 Ala Ser Ala Leu Ser Ala Asn Asn Ser Ala Pro Ser Asn Asp Trp Trp             20 25 30 Asp Ile Pro Tyr Pro Ser Gln Phe Asp Val Lys Ser Leu Lys Thr Gln         35 40 45 Ser Phe Ile Ser Val Lys Gly Asn Lys Phe Ile Asp Asp Lys Gly Lys     50 55 60 Thr Phe Thr Phe Arg Gly Val Asn Ile Ala Asp Thr Gly Lys Leu Leu 65 70 75 80 Ser Gln Asn Gln Trp Gln Lys Ser Leu Phe Glu Glu Leu Ala Asn Asn                 85 90 95 Trp Gly Val Asn Thr Ile Arg Leu Pro Ile His Pro Val Ser Trp Arg             100 105 110 Lys Leu Gly Pro Asp Val Tyr Leu Gly His Ile Asp Glu Ala Val Arg         115 120 125 Trp Ala Asn Asp Leu Gly Ile Tyr Leu Ile Leu Asp Trp His Ser Ile     130 135 140 Gly Tyr Leu Pro Thr Glu Gln Tyr Gln His Pro Met Tyr Asp Thr Thr 145 150 155 160 Ile Lys Glu Thr Arg Asp Phe Trp Arg Arg Ile Thr Phe Arg Tyr Lys                 165 170 175 Asn Val Pro Thr Val Ala Val Tyr Glu Leu Phe Asn Glu Pro Thr Thr             180 185 190 Met Gly Asn Thr Leu Gly Glu Arg Asn Trp Ala Glu Trp Lys Thr Leu         195 200 205 Asn Glu Ser Leu Ile Asp Met Ile Tyr Ala Ser Asp Lys Thr Val Ile     210 215 220 Pro Leu Val Ala Gly Phe Asn Trp Ala Tyr Asp Leu Ser Pro Ile Lys 225 230 235 240 Lys Ala Pro Ile Glu Arg Glu Gly Ile Ala Tyr Ala Ala His Pro Tyr                 245 250 255 Pro Gln Lys Ala Lys Pro Glu Val Lys Asn Asp Lys Asn Phe Phe Lys             260 265 270 Leu Trp Asp Glu Lys Trp Gly Phe Ala Ala Asp Thr Tyr Pro Val Ile         275 280 285 Ala Thr Glu Leu Gly Trp Val Gln Pro Asp Gly Tyr Gly Ala His Ile     290 295 300 Pro Val Lys Asp Asp Gly Ser Tyr Gly Pro Arg Ile Val Lys Tyr Met 305 310 315 320 Gln Lys Lys Gly Val Ser Tyr Thr Val Trp Val Phe Asp Pro Asp Trp                 325 330 335 Ser Pro Thr Met Ile Asn Asp Trp Asp Phe Thr Pro Ser Glu Gln Gly             340 345 350 Ala Phe Phe Lys Gln Val Met Leu Glu Ala Lys Lys Arg         355 360 365 <210> 14 <211> 1098 <212> DNA <213> Microbulbifer degradans <400> 14 atgcgcacaa ccaaatttct tgcgcttgca ctctgcttgc tggcctcagc cagtgcactg 60 agtgcaaata acagcgcccc atcaaacgac tggtgggata taccctaccc gagccaattc 120 gatgtaaaaa gccttaaaac gcaaagtttt atatcggtaa aaggtaacaa gttcattgat 180 gataagggca aaaccttcac ttttagaggg gtaaacattg ccgatacagg taagctactt 240 agccaaaatc aatggcaaaa atcgctgttt gaagagctgg ctaataactg gggggtaaat 300 actattcgcc tgcctattca ccctgtaagt tggcgtaaac ttgggccaga cgtttattta 360 ggccacatcg atgaggcggt acgctgggcg aatgatttag gtatttacct tattcttgat 420 tggcactcca ttggctattt gcccaccgag caataccaac accccatgta cgacaccacc 480 attaaagaaa cccgcgactt ttggcgcaga attacgttcc gctacaaaaa cgtgcccacc 540 gtagcggtat acgaattatt taatgagcca accaccatgg gtaacaccct aggcgaacgc 600 aactgggccg agtggaaaac cttaaatgaa agcctaattg atatgatata tgccagtgac 660 aaaaccgtca ttccgctggt tgcaggcttc aactgggcct atgatttatc gccaatcaaa 720 aaggcaccta tcgagcgtga aggcattgct tacgccgcac acccctaccc gcaaaaggcg 780 aaaccagagg ttaagaacga taaaaacttc ttcaaactgt gggacgaaaa gtggggcttt 840 gctgcagaca cctaccctgt aatagcaaca gagctaggct gggtacaacc cgatggttat 900 ggtgcccaca tacccgttaa agacgacggc agttacggcc cccgcatagt gaagtatatg 960 cagaaaaaag gcgtttctta cacggtatgg gtattcgacc ccgactggag cccaacaatg 1020 attaacgact gggattttac ccccagcgag caaggcgcgt tttttaaaca ggttatgcta 1080 gaagctaaaa aacgctaa 1098 <210> 15 <211> 638 <212> PRT <213> Microbulbifer degradans <400> 15 Met Thr Phe Thr Arg Met Lys Ser Ser His Gln Gly Ala Cys Arg Pro 1 5 10 15 Arg Ser Ser Thr Leu Gln Arg Leu Ile Ala Ser Ser Leu Thr Thr Ala             20 25 30 Cys Leu Leu Ala Ala Ser Thr Phe Ala Asp Val Ala Pro Leu Thr Val         35 40 45 Asp Gly Asn Arg Ile Leu Ser Gly Gly Gln Glu Ala Ser Phe Ala Gly     50 55 60 Asn Ser Leu Phe Trp Ser Asn Asn Tyr Trp Gly Gly Glu Lys Tyr Tyr 65 70 75 80 Thr Ala Glu Thr Val Asn Trp Leu Lys Gln Asp Trp Gly Ala Thr Leu                 85 90 95 Val Arg Ala Ala Met Gly Val Glu Asp Asn Gly Gly Tyr Leu Asp Asp             100 105 110 Lys Glu Gly Asn Lys Gln Lys Val Lys Thr Val Val Asp Ala Ala Ile         115 120 125 Ala Asn Asp Met Tyr Val Ile Ile Asp Trp His Ser His His Ala Glu     130 135 140 Asp His Lys Ser Glu Ala Ile Ala Phe Phe Glu Asp Met Ala Arg Thr 145 150 155 160 Tyr Gly Asn Lys Lys His Val Ile Tyr Glu Ile Tyr Asn Glu Pro Leu                 165 170 175 Gln Ile Ser Trp Ser Asn Thr Ile Lys Pro Tyr Ala Glu Asp Val Ile             180 185 190 Arg Ala Ile Arg Ala Ile Asp Pro Asp Asn Leu Ile Val Gly Thr         195 200 205 Pro Thr Trp Ser Gln Asp Val Asp Val Ala Ser Gln Asp Pro Ile Thr     210 215 220 Gly Tyr Ala Asn Ile Ala Tyr Thr Leu His Phe Tyr Ala Gly Thr His 225 230 235 240 Lys Gln Ser Leu Arg Asp Lys Ala Gln Thr Ala Leu Asn Asly Gly Ile                 245 250 255 Ala Leu Phe Ala Thr Glu Trp Gly Thr Val Asn Ala Asn Gly Asp Gly             260 265 270 Ala Val Asn Thr Thr Glu Thr Asp Lys Trp Met Thr Phe Phe Lys Thr         275 280 285 Asn His Ile Ser His Ala Asn Trp Ala Leu Asn Asp Lys Ser Glu Gly     290 295 300 Ala Ser Ala Leu Asn Pro Gly Ala Ser Pro Asn Gly Asn Trp Ser Asn 305 310 315 320 Ala Asp Leu Thr Thr Ser Gly Lys Tyr Val Lys Asn Ile Ile Lys Asn                 325 330 335 Trp Asn Asp Gly Thr Pro Gly Gly Ser Ser Ser Ser Ser Ser Gly Gly             340 345 350 Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Asn Ser Ser Ser Gly         355 360 365 Ala Gly Lys Val Asn Leu Pro Ala Arg Ile Glu Ala Glu Asn Tyr Asn     370 375 380 Ser Ala Pro Val Glu Thr Thr Ala Gly Asn Ser Gly Gly Ser Val Ser 385 390 395 400 Gln Cys Thr Tyr Arg Gly Leu Asn Val Asp Val Gln Asp Ala Ser Glu                 405 410 415 Gly Thr Cys Asn Ile Gly Trp Thr Ala Ala Gly Glu Lys Val Thr Tyr             420 425 430 Asn Ile Gly Thr Ala Asn Asn Thr Tyr Asn Ile Ala Leu Arg Thr Ala         435 440 445 Ser Leu Asp Ala Gly Lys Arg Val Ser Val Tyr Val Gly Asn Thr Leu     450 455 460 Ala Asp Thr Ile Ser Thr Gln Gly Gly Gly Trp Gln Asn Trp Lys Thr 465 470 475 480 Gln Thr Ile Pro Asn Val Tyr Ile Pro Ser Asn Ser Val Ile Thr Val                 485 490 495 Glu Phe Tyr Asp Gly Arg Thr Asn Leu Asn Tyr Leu Asn Ile Ser Ala             500 505 510 Ala Ser Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser         515 520 525 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly Gly Gly Ser Cys     530 535 540 Ser Ser Tyr Ile Asp Ile Pro Trp Asn Thr Arg Thr Glu Val Thr Leu 545 550 555 560 Thr Ser Gly Ala Cys Val Arg Phe Asn Gln Asn Leu Ser Gly Lys Thr                 565 570 575 Leu Gln Val Trp Asp Ser Asp Ala Asn Ser Ser Cys Asp Phe Arg Gly             580 585 590 Thr Val Thr Thr Val Gly Gly Thr Gly Ser Leu Asn Val Ser Ser Asn         595 600 605 Tyr Val Ser Ser Lys Ser Leu Thr Gly Thr Lys Leu Thr Phe Asn Ser     610 615 620 Ala Ser Asn Asn Asn Cys Lys Tyr Val Lys Val Arg Ala Tyr 625 630 635 <210> 16 <211> 1917 <212> DNA <213> Microbulbifer degradans <400> 16 atgactttca caagaatgaa atcatcacac caaggcgcgt gtcgaccaag gtcttccacc 60 ctacagcgac taatcgcctc atcacttacc accgcatgtt tgctagcagc gtctactttt 120 gccgacgtag cgccgttaac cgtagatggc aaccgcattc tcagcggtgg ccaagaggct 180 agctttgccg gtaacagttt gttttggagc aacaattatt ggggcggtga gaaatactac 240 acagccgaaa ctgttaactg gttaaaacaa gactggggcg caacactagt gcgcgcggcc 300 atgggtgtag aagataacgg cggctaccta gatgacaaag aaggcaacaa acaaaaggta 360 aaaaccgttg tagatgctgc tattgccaac gacatgtatg taattatcga ttggcacagc 420 caccacgccg aagaccacaa aagtgaagcc attgcttttt ttgaggatat ggcgcgcacc 480 tacggcaata aaaaacacgt tatttacgaa atttataacg agcctttaca aatttcgtgg 540 agcaacacaa ttaaacccta cgccgaagat gtaattagag ctattcgcgc gatagacccc 600 gacaacttaa ttattgttgg tacgccaacg tggtcgcaag atgtagacgt agcatcgcaa 660 gaccccatta ccggctacgc caatattgcc tacacattgc acttttacgc aggcacccac 720 aaacaatctt tacgagacaa agcgcaaacc gcacttaaca acggcatagc gcttttcgca 780 acagagtggg gaacagtaaa tgcaaacggt gatggcgctg taaacaccac cgaaacagac 840 aagtggatga cgttctttaa aaccaaccac ataagccacg caaactgggc gctaaacgac 900 aaatcagaag gcgcttctgc attaaacccc ggagccagcc ccaatggcaa ctggagcaac 960 gccgacttaa ccacatcggg taagtacgta aaaaacatta tcaaaaactg gaacgacggc 1020 acgccgggag gcagctcttc aagctcgtcc ggcggctcaa ccagttcctc ctcaagctca 1080 tctagctcta attccagctc tggtgctggc aaagtaaatt tacccgcacg cattgaagcc 1140 gaaaactata acagtgcacc ggtagaaaca actgcaggca atagtggcgg cagcgtttca 1200 caatgtacat acagagggct aaatgtagac gtacaagacg caagcgaagg cacttgtaat 1260 attggctgga cagcagcagg cgaaaaagtt acctacaaca taggcacagc aaataatact 1320 tacaatattg cacttcgcac cgcatcgctt gatgcaggca agcgcgtatc ggtatatgta 1380 ggcaacaccc tcgccgacac aataagcacc caaggtggcg gctggcaaaa ttggaagacg 1440 caaaccatcc ccaatgtata tattccatca aactcagtta ttaccgtgga attctacgat 1500 ggccgcacca accttaacta cttaaacatt agtgcagctt cggggtcttc ctcttcaagc 1560 tcctcatcta gctcgtcaac gtctagctct tcttcgagct catcttctag ctcttcaggt 1620 ggtggcagtt gtagcagcta tatagatata ccttggaata ctcgcaccga agttacccta 1680 acaagtggcg cctgcgttcg ctttaaccaa aacctttcgg gcaaaaccct acaagtgtgg 1740 gatagcgatg caaactcatc gtgcgatttc cggggcacag ttacaacagt aggcggcact 1800 ggcagtttaa atgtaagcag caactatgtt tcgtctaaga gcctaacagg aaccaaactt 1860 acatttaatt cagcaagtaa taacaattgt aagtacgtta aagttcgtgc ttattag 1917 <210> 17 <211> 630 <212> PRT <213> Microbulbifer degradans <400> 17 Met Lys Ser Ala Thr Thr Asn Gln Ser Arg Ala Arg Ser Ser Ala Phe 1 5 10 15 Lys Asn Met Leu Ala Ala Ser Leu Ala Gly Leu Gly Leu Leu Ser Ala             20 25 30 Ser Ala Phe Ala Asp Val Ala Pro Leu Thr Val Asp Gly Asn Lys Ile         35 40 45 Leu Ser Gly Gly Gln Gln Ala Ser Phe Ala Gly Asn Ser Leu Phe Trp     50 55 60 Ser Asn Asn Gly Trp Gly Gly Glu Lys Tyr Tyr Thr Ala Gly Thr Val 65 70 75 80 Glu Trp Leu Lys Gln Asp Trp Gly Ser Asn Leu Val Arg Ala Ala Met                 85 90 95 Gly Val Asp Glu Asn Gly Gly Tyr Leu Glu Asp Pro Ala Gly Asn Lys             100 105 110 Ala Lys Val Thr Thr Val Val Asp Ala Ala Ile Ala Asn Asp Met Tyr         115 120 125 Val Ile Ile Asp Trp His Ser His His Ala Glu Asp Tyr Gln Asn Gln     130 135 140 Ala Ile Ser Phe Phe Gln Asp Met Ala Arg Thr Tyr Gly Asn Asn Asn 145 150 155 160 Asn Val Ile Tyr Glu Ile Tyr Asn Glu Pro Leu Gln Val Ser Trp Ser                 165 170 175 Gly Thr Ile Lys Pro Tyr Ala Glu Ala Val Ile Gly Ala Ile Arg Ala             180 185 190 Ile Asp Pro Asp Asn Leu Ile Ile Val Gly Thr Pro Thr Trp Ser Gln         195 200 205 Asp Val Asp Val Ala Ser Arg Asp Pro Ile Thr Gln Tyr Ser Asn Ile     210 215 220 Ala Tyr Thr Ile His Phe Tyr Ala Gly Thr His Lys Gln Ser Leu Arg 225 230 235 240 Asp Lys Ala Gln Thr Ala Leu Asn Asn Gly Ile Ala Leu Phe Ala Thr                 245 250 255 Glu Trp Gly Thr Val Asn Ala Asn Gly Asp Gly Gly Val Asp Ala Ala             260 265 270 Glu Thr Asp Arg Trp Met Gln Phe Phe Lys Ala Asn His Ile Ser His         275 280 285 Ala Asn Trp Ala Leu Asn Asp Lys Ala Glu Gly Ser Ser Ala Leu Lys     290 295 300 Pro Gly Ser Asn Ala Asn Gly Gly Trp Ser Asn Ser Asp Leu Thr Ala 305 310 315 320 Ser Gly Thr Tyr Val Lys Asn Leu Ile Lys Thr Trp Asn Asp Gly Ser                 325 330 335 Pro Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser             340 345 350 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly         355 360 365 Gly Thr Asn Leu Pro Ala Arg Ile Glu Ala Glu Asn Tyr Asp Ser Ala     370 375 380 Pro Val Glu Thr Thr Ala Gly Asn Ser Gly Ser Pro Thr Asn Cys Ser 385 390 395 400 Tyr Lys Gly Met Gly Val Asp Val Glu Asn Ser Thr Glu Gly Ala Cys                 405 410 415 Asn Ile Gly Trp Thr Ala Ala Gly Glu Lys Val Thr Tyr Asn Ile Gly             420 425 430 Asn Ala Asp Gly Thr Tyr Asp Ile Ala Leu Arg Val Ala Ser Met Asp         435 440 445 Ala Gly Lys Arg Ile Ser Val His Val Asn Asn Ser Leu Ala Asp Thr     450 455 460 Val Thr Thr Gln Gly Gly Gly Trp Gln Ala Trp Thr Thr Glu Thr Ile 465 470 475 480 Ser Asn Val Tyr Ile Pro Ser Asn Ser Val Ile Thr Val Glu Phe Tyr                 485 490 495 Asp Ser Gly Ser Asn Leu Asn Phe Leu Asn Ile Thr Glu Ser Ser Gly             500 505 510 Thr Glu Pro Pro Val Glu Pro Pro Val Glu Pro Pro Val Glu Pro Pro         515 520 525 Val Asp Asn Gly Asn Phe Pro Cys Asn Asp Gly Asn Ser Thr Leu Ala     530 535 540 Asn Asn Gly Ala Ser Ile Asn Leu Asn Gln Gly Ala Cys Val Lys Tyr 545 550 555 560 Asn His Gly Trp Gly Asp Ile Arg Leu Gly Thr Trp Ser Gly Asn Gly                 565 570 575 Thr Ile Arg Tyr Asp Val Leu Asp Cys Asn Asn Asn Val Met Ser Asp             580 585 590 Ile Ala Gln Lys Leu Asn Asp Phe Thr Ala Val Asp Thr Ala Thr Met         595 600 605 Asn Cys Ala His Tyr Ile Tyr Val Lys Gln Ala Pro Ser Ser Tyr Thr     610 615 620 Leu Gln Phe Gly Ser Trp 625 630 <210> 18 <211> 1893 <212> DNA <213> Microbulbifer degradans <400> 18 atgaaatcag caaccacaaa tcaatcgagg gcacgcagta gcgcctttaa aaatatgttg 60 gcggcatcgc tcgcaggttt agggctacta tcagcttctg catttgccga tgtagccccg 120 ctaaccgtag acggcaataa aattcttagc ggtggccagc aagccagttt tgccggtaat 180 agcttatttt ggtctaacaa tggctggggc ggtgagaagt attacacggc cggtaccgtt 240 gaatggctaa agcaagactg gggcagtaat ttagttcgcg ccgcaatggg tgtcgatgaa 300 aacggcggct acttagaaga cccagcagga aacaaagcga aagtaacaac cgttgtagat 360 gcagccatcg ctaacgatat gtatgtaatt atcgattggc acagccacca cgccgaagac 420 taccaaaacc aagccattag ctttttccaa gatatggctc gcacctacgg taacaacaac 480 aacgttatat acgaaattta taacgagcca ttacaggttt cttggagcgg caccatcaag 540 ccttacgcag aagcggtaat tggcgcaatt cgcgcaatcg acccagataa ccttattatt 600 gtgggcacgc ctacttggtc gcaggatgta gacgtagcct cgcgcgaccc catcacgcag 660 tacagcaaca ttgcctacac tattcacttt tatgcgggca cccacaaaca atccctacgc 720 gataaagcac aaaccgcatt aaataatggt attgctttgt ttgctaccga atggggtaca 780 gtaaatgcca acggtgacgg cggtgtagac gcagccgaaa ctgatcgttg gatgcagttt 840 tttaaagcga atcatataag ccatgccaac tgggccttaa acgataaagc cgaaggctct 900 tctgcattaa agcctggctc taacgcaaac ggcggctgga gcaattccga cttaaccgcc 960 tctggtacct atgttaaaaa cttaattaaa acatggaacg acggctcacc gagcagcagc 1020 tcatctagca gcaccagttc ttcttcaagc agctcctcgt ctagtagctc atcatctagc 1080 agctcttcat ctagtagttc tggcggtacc aatttacccg cgcgcattga agcagaaaac 1140 tacgatagcg caccggtaga aaccactgca ggtaatagcg gctcacccac caattgttcg 1200 tataaaggta tgggcgtaga tgtagaaaac tctactgaag gtgcttgtaa tattggctgg 1260 actgcggcag gcgaaaaagt aacttacaac attggcaatg ccgatggcac ttacgatatt 1320 gcattgcgcg tagcctctat ggatgcgggc aaacgtatct ctgtgcatgt aaacaacagc 1380 ctagcagata ccgtaaccac acaaggtggc ggctggcagg catggactac cgaaaccatt 1440 tctaacgtgt atatcccatc aaactcggta attaccgttg agttttacga tagtggctct 1500 aacctaaact ttttaaacat taccgaaagc tcgggtaccg aaccacctgt agaaccaccc 1560 gttgagccgc cagtagaacc acccgtagac aacggtaact tcccatgtaa cgacggtaac 1620 tctacgcttg ccaacaacgg cgcctccatt aaccttaacc aaggagcgtg tgttaaatac 1680 aatcacggct ggggcgatat tcgtttaggc acctggagcg gcaacggtac cattcgatac 1740 gacgtactag actgcaataa caacgtaatg agtgatattg cacaaaaact taatgacttt 1800 actgctgtag acaccgcaac aatgaactgc gcacactaca tttatgtaaa acaagcccct 1860 agcagctaca ccctgcaatt tggtagctgg tag 1893 <210> 19 <211> 725 <212> PRT <213> Microbulbifer degradans <400> 19 Met Lys Ile Asn Thr Leu Phe Thr Pro Leu Arg Thr Val Gly Ala Ala 1 5 10 15 Val Ala Ile Ala Leu Ser Pro Val Ala Phe Ala Asp Val Thr Cys Glu             20 25 30 Val Thr Asn Phe Asn Gln Trp Asn Ser Gly Tyr Gln Ala Asp Val Arg         35 40 45 Val Thr Asn Ser Gly Ser Ala Val Ser Gly Trp Thr Val Asn Leu Asn     50 55 60 Phe Ala Ser Ala Pro Gln Met Thr Asn Gly Trp Asn Ala Ala Leu Ser 65 70 75 80 Thr Ser Gly Asn Thr Ile Ser Ala Ser Asn Ile Ser Trp Asn Gly Asn                 85 90 95 Leu Gly Asn Gly Gln Ser Thr Ser Phe Gly Phe Gln Gly Asn Ser Asn             100 105 110 Gly Asn Leu Ala Thr Pro Thr Cys Val Gly Ser Gly Thr Gly Ser Ser         115 120 125 Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser Thr     130 135 140 Ser Ser Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser Ser Ser Gly 145 150 155 160 Gly Glu Cys Val Glu Met Cys Lys Trp Tyr Gln Asp Ala Pro Arg Pro                 165 170 175 Leu Cys Asn Asn Gln Asp Ser Gly Trp Gly Trp Glu Asn Asn Gln Ser             180 185 190 Cys Ile Gly Arg Thr Thr Cys Asn Ser Gln Ser Gly Asn Gly Gly Val         195 200 205 Ile Asn Ser Cys Pro Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr     210 215 220 Ser Ser Thr Ser Ser Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser Thr 225 230 235 240 Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser                 245 250 255 Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser Ser Ser Gly Gly Gly Val             260 265 270 Phe Arg Val Asp Ala Thr Gly Asn Ile Thr Lys Asn Gly Val Luu         275 280 285 Pro Val Arg Cys Gly Asn Trp Phe Gly Leu Glu Gly Gln His Glu Pro     290 295 300 Ser Asp Ala Gln Asn Asn Pro Gly Gly Ala Pro Leu Glu Leu Tyr Val 305 310 315 320 Gly Asn Met Trp Trp Val Asp Ser Gly Arg Thr Ile Gln Gln Thr Met                 325 330 335 Ser Glu Ile Thr Ala Gln Gly Ile Asn Met Val Arg Leu Pro Ile Ala             340 345 350 Pro Gln Thr Leu Asn Pro Asn Asp Pro Gln Gly Val Gly Asp Val Arg         355 360 365 Asn Gly Gly Val Leu Lys Asn His Glu Ser Val Gln Gln Thr Asn Ala     370 375 380 Arg Gln Ala Leu Glu Asp Phe Ile Val Gln Ala Asn Glu Asn Asp Ile 385 390 395 400 Gln Val Leu Ile Asp Ile His Ser Cys Ser Asn Tyr Val Gly Trp Arg                 405 410 415 Ala Gly Arg Leu Asp Ala Glu Pro Pro Tyr Val Asp Ala Thr Arg Val             420 425 430 Gly Tyr Asp Phe Thr Arg Glu Asp Tyr Ser Cys Gly Thr Asn Val Gly         435 440 445 Pro Gly Val Thr Val His Glu Tyr Asn Glu Glu Ile Trp Leu Asn Asn     450 455 460 Leu Arg Glu Ile Ala Gly Leu Ser Glu Ser Leu Gly Val Asp Asn Ile 465 470 475 480 Ile Gly Ile Asp Ile Phe Asn Glu Pro Trp Asp Tyr Thr Trp Glu Glu                 485 490 495 Trp Lys Ala Leu Ser Glu Ser Ala Tyr Gln Ala Ile Ser Glu Val Asn             500 505 510 Pro Asp Ile Leu Ile Phe Val Glu Gly Val Ala Gly Gly Thr Gly Ala         515 520 525 Gly Val Asp Val Pro His Gly Asp Glu Ser Ser Asn Pro Asn Trp Gly     530 535 540 Glu Asn Phe Tyr Pro Ala Gln Thr Ala Pro Leu Asn Ile Pro Lys Asp 545 550 555 560 Arg Leu Val Ile Ser Pro His Thr Tyr Gly Pro Ser Val Phe Val Gln                 565 570 575 Arg Gln Phe Met Asp Pro Asn Asp Pro Glu Cys Val Gly Leu Glu Gly             580 585 590 Asp Glu Ala Ala Glu Ala Gly Cys Gln Ile Val Ile Asp Tyr Ala Thr         595 600 605 Leu Ala Ala Gly Trp Asp Glu His Phe Gly Phe Leu Arg Glu Gln Gly     610 615 620 Phe Ala Met Val Val Gly Glu Phe Gly Gly Asn Met Asp Trp Pro Asn 625 630 635 640 Gly Thr Arg Gln Ala Glu Lys Asp Met Trp Ser His Ile Thr Pro Gly                 645 650 655 Ile Asp Arg Gln Trp Gln Glu Ala Phe Val Asp Tyr Met Val Glu Lys             660 665 670 Asn Ile Gln Ala Cys Tyr Trp Ser Ile Asn Pro Glu Ser Gly Asp Thr         675 680 685 Gly Gly Trp Tyr Gly His Glu Tyr Asp Pro Val Ser Asn Asp Ala Gly     690 695 700 Trp Gly Arg Trp Leu Asp Phe Asp Ser Arg Lys Thr Asn Leu Leu Lys 705 710 715 720 Glu Leu Trp Gly Ile                 725 <210> 20 <211> 71 <212> PRT <213> Microbulbifer degradans <400> 20 Met Leu Glu Val Glu Leu Leu Leu Val Glu Leu Val Glu Leu Asp Glu 1 5 10 15 Val Leu Glu Val Leu Leu Val Glu Leu Asp Glu Val Leu Glu Val Leu             20 25 30 Asp Glu Leu Val Asp Glu Val Leu Glu Glu Leu Glu Glu Leu Glu Glu         35 40 45 Leu Gly Gln Leu Leu Ile Thr Pro Pro Leu Pro Asp Trp Leu Leu Gln     50 55 60 Val Val Arg Pro Ile Gln Leu 65 70 <210> 21 <211> 2178 <212> DNA <213> Microbulbifer degradans <400> 21 atgaaaatca acactctctt tacgcctttg cgtactgtgg gtgctgcagt tgcgatagct 60 ttatcgcctg tagcctttgc agacgtaacg tgcgaagtaa cgaactttaa ccagtggaat 120 agtggctacc aagccgatgt tcgtgttaca aacagcggta gcgctgttag tggctggacc 180 gtaaatttaa attttgcctc agccccgcaa atgacaaatg gctggaacgc agctttgagt 240 actagcggca atacaattag tgcatctaat attagttgga atggcaattt gggtaatggt 300 cagtccacca gctttggttt tcagggcaat tcaaatggta acttggcaac gccaacgtgt 360 gtaggtagcg gtacggggtc ttctagcagc tcttcatcca gctctacttc tagcacaagc 420 tcatcatcta caagttcttc tagcacgtct tctactagct ctagcagttc atcctctggt 480 ggtgaatgtg tagaaatgtg taagtggtat caagatgcac cgcgcccatt atgtaataat 540 caagacagtg gttggggttg ggaaaacaat caaagctgta ttggtcgcac tacttgtaac 600 agccaatctg gcaatggtgg tgtaattaat agttgcccaa gttcttcaag ttcttcaagt 660 tcttctagca cttcgtctac cagctcatct agtacttcaa gtacttcatc gagctcaaca 720 agtagtactt caagcacttc atcaagttcc acaagctcta ctagcagcag ctcaacctct 780 agcactagct cgtcgtcttc aagtggtggt ggagtattcc gcgtagatgc taccggtaat 840 attactaaaa atggtgaagt actgcctgtt cgttgtggta actggtttgg tctagagggc 900 cagcacgagc cttcagatgc gcaaaataac ccaggcggtg cgccgcttga attatatgtt 960 ggcaacatgt ggtgggtaga tagtggccgc actattcagc aaaccatgag cgaaattacc 1020 gcccaaggta tcaacatggt tcgcttgcct attgcaccgc aaacattaaa ccctaacgac 1080 cctcaaggtg tgggtgatgt gcgcaacggc ggcgtgctta aaaatcacga atctgtgcag 1140 caaaccaatg cacgtcaagc gttagaagac ttcattgttc aagctaacga aaatgacatt 1200 caagtgctaa ttgatattca ctcttgtagt aactacgtgg gttggcgtgc aggccgttta 1260 gatgcagagc ctccttatgt ggatgcaacg cgagtgggtt atgactttac ccgtgaagat 1320 tattcttgtg gcaccaatgt gggcccaggt gtaactgtgc acgagtacaa cgaggaaatt 1380 tggttaaaca acttgcgtga gattgctggt ttatctgaat ccttgggcgt tgataatatt 1440 atcggtatcg atatttttaa cgaaccatgg gattacactt gggaagagtg gaaagcactt 1500 tctgaaagcg cttatcaagc cattagcgaa gttaacccag atattctaat ctttgttgag 1560 ggtgttgcag gcggcacggg tgctggtgtt gatgtgccac atggagacga gtcttctaac 1620 cctaactggg gcgaaaactt ttatcctgcg caaactgctc cgcttaatat tccaaaagat 1680 cgtctagtta tttcaccgca tacctatggc ccatctgtat ttgttcagcg tcaatttatg 1740 gacccgaatg atccagagtg tgttggttta gaaggtgatg aggcggctga agctggctgt 1800 caaattgtta tcgattatgc aaccttagca gctggttggg atgagcattt cggcttctta 1860 cgtgagcaag gctttgccat ggtagtgggt gagtttggtg gcaacatgga ttggccaaat 1920 ggcacgcgcc aagcagaaaa agatatgtgg agccacatca cccctggaat cgacagacag 1980 tggcaagaag cgtttgttga ctacatggtt gagaaaaaca tccaagcttg ttactggtca 2040 attaacccag agtctggcga cactggcggt tggtatggtc acgagtacga ccctgtttct 2100 aacgatgcag gttgggggcg ttggttagac ttcgattctc gcaaaactaa cttacttaaa 2160 gagctttggg gtatttaa 2178 <210> 22 <211> 610 <212> PRT <213> Microbulbifer degradans <400> 22 Met Met Tyr Thr Asn Leu Phe Asn Leu Lys Lys His Leu Phe Gln Thr 1 5 10 15 Ser Leu Lys Leu Leu Ala Cys Ala Thr Leu Ile Gly Gly Thr Leu Asn             20 25 30 Ala Ala Ala Asp Val Pro Ala Met Ser Val Gln Gly Asn Lys Val Leu         35 40 45 Val Gly Gly Glu Val Lys Ser Leu Gly Gly Met Ser Tyr Phe Trp Ser     50 55 60 Asn Asn Gly Trp Gly Gly Glu Lys Tyr Tyr Asn Ala Ser Thr Val Ser 65 70 75 80 Tyr Phe Lys Gln Asp Trp Lys Ala Ser Ile Val Arg Ala Ala Met Gly                 85 90 95 Val Glu Asp Ala Gly Gly Tyr Phe Asp Asp Pro Gln Gly Ser Lys Gln             100 105 110 Lys Val Arg Thr Ile Val Asp Ala Ala Ile Ala Asn Asp Met Tyr Val         115 120 125 Ile Ile Asp Trp His Ser His Tyr Ala Asn Thr His Asp Trp Ala Ala     130 135 140 Ala Val Gln Phe Phe Gln Glu Met Ala Arg Asp Tyr Gly Gln Tyr Asn 145 150 155 160 Asn Val Ile Tyr Glu Val Tyr Asn Glu Pro Leu Asp Ile Pro Trp Gly                 165 170 175 His Ile Lys Ser Tyr Ala Glu Thr Val Ile Asp Ala Ile Arg Ala Ile             180 185 190 Asp Pro Asp Asn Val Ile Val Val Gly Thr Pro Arg Trp Ser Gln Gly         195 200 205 Val Lys Glu Ala Ser Trp Asp Pro Ile Asn Arg Asn Asn Ile Ala Tyr     210 215 220 Thr Leu His Phe Tyr Ser Gly Ser His Gly Gln Trp Leu Arg Asn Asp 225 230 235 240 Ala Ala Glu Ala Met Ser Asn Gly Ile Ala Leu Phe Val Thr Glu Trp                 245 250 255 Gly Ser Val Asn Ala Asn Gly Asp Gly Ala Val Asn Glu Gly Glu Thr             260 265 270 Ala Ala Trp Met Asn Phe Met Arg Asp Asn Gly Ile His His Ala Asn         275 280 285 Trp Ser Val Asn Asp Lys Ala Glu Gly Ala Ser Ala Leu Asn Pro Gly     290 295 300 Ala Ser Ala Thr Gly Gly Trp Gly Asp Gly Asp Leu Thr Trp Ser Gly 305 310 315 320 His Val Val Arg Gly Tyr Leu Arg Asp Trp Asn Gln Ile Gly Ser Gly                 325 330 335 Asn Gly Asn Gly Asn Gly Thr Gly Cys Thr Glu Val Ser Leu Pro Gly             340 345 350 Thr Ile Glu Ala Glu Ala Tyr Cys Ala Met Asp Gly Ile Gln Thr Glu         355 360 365 Asn Thr Asn Asp Thr Asn Gly Gly Ser Asn Val Gly Tyr Ile Asp Ala     370 375 380 Gly Asp Trp Met Ser Tyr Ser Val Asn Val Ala Asn Ala Gly Thr Tyr 385 390 395 400 Thr Val Ser Tyr Arg Val Ala Ser Leu Gly Gly Gly Gly Val Leu Ser                 405 410 415 Ile Glu Asn Ala Gly Gly Ser Pro Val Tyr Gly Thr Leu Asn Val Pro             420 425 430 Gln Thr Gly Gly Trp Gln Glu Trp Thr Thr Val Ser His Asp Ile Ser         435 440 445 Leu Gln Ala Gly Gln Gln Asn Ile Gly Ile Ala Ala Ile Glu Gly Gly     450 455 460 Phe Asn Ile Asn Trp Ile Ala Leu Thr Pro Ala Gly Thr Asn Pro Asn 465 470 475 480 Pro Val Gln Ser Ile Thr Leu Gln Ala Glu Asp Tyr Ser Phe Met Ser                 485 490 495 Gly Val Gln Val Glu Asn Thr Ser Asp Asn Gly Gly Gly Met Asn Val             500 505 510 Gly Trp Leu Asp Ala Gly Asp Trp Leu Ala Tyr His Gly Val Asn Ile         515 520 525 Pro Thr Ser Gly Gln Tyr Thr Ile Thr Tyr Arg Val Ala Ser Gln Ser     530 535 540 Gly Gly Gly Ser Leu Gln Leu Glu Gln Ala Gly Gly G Val Val Tyr 545 550 555 560 Gly Asn Leu Asn Val Pro Ser Thr Gly Gly Trp Gln Asn Trp Val Asp                 565 570 575 Val Ser His Thr Val Thr Leu Asn Ala Gly Val Gln Asp Phe Gly Leu             580 585 590 Gly Ile Thr Ser Gly Gly Phe Asn Ile Asn Trp Ile Lys Val Glu Ala         595 600 605 Ile his     610 <210> 23 <211> 1833 <212> DNA <213> Microbulbifer degradans <400> 23 atgatgtaca caaacctctt taatttaaaa aagcacctct ttcaaacctc acttaaacta 60 ctggcctgcg ccacattaat tggcggcacc ctaaacgcag ccgctgacgt gccagcaatg 120 tccgtacaag gcaataaagt actggtgggc ggtgaagtta aaagccttgg aggtatgagc 180 tatttttggt ctaacaacgg ctggggcggc gagaaatact acaacgcttc taccgttagt 240 tacttcaagc aagactggaa ggcatccatt gttcgagctg caatgggggt agaagatgcc 300 ggcggctact tcgatgaccc gcagggctct aagcaaaaag ttcgtacaat agtagatgcc 360 gccattgcga atgatatgta cgtcattatc gattggcact cacattacgc caacacccac 420 gactgggcag ccgctgtgca atttttccaa gaaatggcac gtgactatgg ccaatacaat 480 aatgtgattt atgaggtata caacgaacca ctggatatcc cttggggcca cataaaaagc 540 tacgccgaaa cggtaattga tgccattcgc gcaattgacc cagataacgt gatcgtagta 600 ggcactcctc gctggtcgca gggggtaaaa gaagcgtcat gggacccaat caaccgcaat 660 aatattgcct acacgctgca cttctattca ggtagtcatg gccaatggct gcgcaacgac 720 gcagcagaag ctatgagtaa tggtattgcc ttgtttgtta ctgaatgggg cagcgtaaat 780 gccaatggcg atggcgcagt caacgaaggc gaaaccgcag cgtggatgaa cttcatgcgc 840 gataacggta tccatcacgc aaactggtct gtaaacgaca aagcagaggg tgcatctgca 900 cttaaccctg gcgccagtgc cacaggtggt tggggcgacg gcgatttgac ttggtctggc 960 catgttgtgc gcggctacct gcgcgactgg aaccaaattg gttctggcaa tggtaacggc 1020 aacggcacag gctgcaccga ggttagccta ccaggcacga tagaagcgga agcctactgc 1080 gcaatggatg gtatccaaac cgaaaacacc aacgacacca acggcggcag taacgtgggc 1140 tacatagatg ctggcgactg gatgagctac agcgtaaacg ttgctaacgc aggcacttat 1200 accgtgtctt accgcgtggc tagccttggc ggcggcggtg ttctaagcat tgaaaatgcc 1260 ggcggctcgc ccgtttatgg cacgctgaat gtaccgcaaa ctggcggctg gcaagaatgg 1320 accactgtat ctcacgatat tagcttgcaa gccggccaac aaaacattgg catagcggca 1380 atagaaggtg gttttaacat caactggata gccctaaccc ctgctggcac caaccccaac 1440 ccagtgcaaa gtattacctt acaagcagaa gactactcct ttatgagtgg cgtgcaggta 1500 gaaaatacta gcgacaatgg cggcggtatg aacgtaggct ggttagatgc tggcgactgg 1560 cttgcctacc acggcgtaaa cattccaacc tctggccaat acaccataac ttaccgagta 1620 gccagccaaa gcggtggtgg aagcctgcag ctagaacaag caggtggcgg cgttgtttac 1680 ggtaacctga acgtaccaag cactggcggc tggcagaact gggtagacgt aagccatacc 1740 gttaccctta acgctggtgt acaagatttt gggttaggta ttactagtgg tggcttcaat 1800 attaactgga taaaagtcga ggcaattcac taa 1833 <210> 24 <211> 791 <212> PRT <213> Microbulbifer degradans <400> 24 Met Leu Ala Ser Asn Lys Asn Ser Lys Leu Ala Asn Ser Glu Gln His 1 5 10 15 Arg Pro Tyr Lys Thr Arg Thr Ala Arg Trp Leu Thr Gly Ser Gly Val             20 25 30 Ile Ala Ser Ser Leu Leu Phe Ser Ala Gln Ser Phe Ala Ala Gln Cys         35 40 45 Glu Tyr Ile Ile Ser Asn Glu Trp Asn Ser Gly Phe Thr Gly Ala Val     50 55 60 Arg Ile Thr Asn Asn Gly Thr Thr Pro Ile Asn Gly Trp Asp Val Ser 65 70 75 80 Trp Gln Tyr Ala Gly Asp Ala Val Thr Ser Ser Trp Asn Ala Asn Val                 85 90 95 Ser Gly Ser Asn Pro Val Ser Ala Thr Pro Leu Ser Trp Asn Ala Asn             100 105 110 Ile Gln Pro Gly Gln Ser Val Glu Phe Gly Phe Gln Gly Ser Lys Ala         115 120 125 Gly Ser Asn Ala Glu Ile Pro Thr Val Thr Gly Ala Val Cys Asp Ser     130 135 140 Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 145 150 155 160 Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser                 165 170 175 Thr Ser Ser Ser Ser Ser Ser Ser Gly Ser Ser Gly Thr Gly Gly Ile             180 185 190 Ala Cys Thr Val Gly Asn Ala Asn Ile Trp Gly Ser Gly Tyr Gln Leu         195 200 205 Asp Met Gln Val Val Asn Asn Gly Thr Ala Ala Val Ser Ser Trp Asp     210 215 220 Val Thr Met Ala Phe Gly Glu Ala Pro Gln Arg Thr Gly Gly Trp Asn 225 230 235 240 Ala Asn Phe Val Glu Ser Gly Asn Thr Ile Val Ala Ser Asn Ile Ser                 245 250 255 Trp Asn Gly Asn Leu Ala Pro Gly Gln Ser Ala Ser Phe Gly Ile Gln             260 265 270 Gly Asn His Asp Gly Ser Phe Gly Gly Val Thr Cys Asn Gly Ala Ser         275 280 285 Ser Ser Gly Ser Ser Ser Ser Gly Ser Ser Thr Ser Ser Ser Ser Ser     290 295 300 Ser Ser Ser Ser Ser Gly Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser 305 310 315 320 Ser Thr Gly Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr                 325 330 335 Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser Ser             340 345 350 Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser         355 360 365 Ser Ser Ser Thr Ser Gly Ser Gly Ala Gly Phe Asp Asn Pro Phe Ile     370 375 380 Gly Gly Lys Trp Tyr Val Asp Pro Val Trp Ser Ala Lys Ala Ala Ala 385 390 395 400 Glu Pro Asn Gly Ser Leu Ile Ala Asn Tyr Asn Thr Ala Val Trp Met                 405 410 415 Asp Arg Ile Gly Ala Ile Glu Gly Pro Glu Asp Gly Asp Gly Met Gly             420 425 430 Leu Glu Glu His Leu Asp Glu Ala Leu Ala Gln Gly Ala Asp Ile Phe         435 440 445 Met Phe Val Val Tyr Asp Leu Pro Asn Arg Asp Cys Ala Ala Leu Ala     450 455 460 Ser Ser Gly Glu Leu Leu Ile Ala Glu Asn Gly Phe Glu Arg Tyr Gln 465 470 475 480 Asn Glu Tyr Ile Gly Pro Ile Val Asp Ile Leu Ser Lys Pro Ala Tyr                 485 490 495 Ser Ser Leu Arg Ile Ile Ala Ile Ile Glu Val Asp Ser Leu Pro Asn             500 505 510 Leu Val Thr Asn Leu Asn Ile Gln Lys Cys Val Glu Ala Asn Gly Pro         515 520 525 Gly Gly Tyr Val Asp Gly Ile Gln His Ala Leu Asn Glu Leu Asn Thr     530 535 540 Leu Asp Asn Val Tyr Pro Tyr Val Asp Ile Ala His Ser Gly Trp Leu 545 550 555 560 Gly Trp Ser Asp Asn Phe Ala Gly Ala Thr Lys Leu Ile Gly Asp Ala                 565 570 575 Ile Lys Gly Thr Asn Lys Gly Val Asn Ser Ile Ala Gly Phe Val Ser             580 585 590 Asn Ser Ser Asn Tyr Thr Pro Val Thr Glu Pro Tyr Leu Pro Asn Pro         595 600 605 Thr Leu Gln Ile Gly Ser Asn Gln Val Arg Ser Ala Asp Phe Tyr Glu     610 615 620 Trp Thr Met Tyr Phe Glu Glu Leu Ser Phe Val Gln Asp Trp Arg Gln 625 630 635 640 Ala Met Ile Gln Gln Gly Phe Pro Glu Ser Ile Gly Met Leu Ile Asp                 645 650 655 Thr Ala Arg Asn Gly Trp Gly Gly Pro Asp Arg Pro Thr Gly Glu Ser             660 665 670 Thr Ser Thr Asp Leu Asn Thr Tyr Val Asn Glu Ser Arg Ile Asp Arg         675 680 685 Arg Gln His Arg Gly Asn Trp Cys Asn Gln Pro Gly Gly Val Gly Phe     690 695 700 Arg Pro Gln Ala Ala Pro Glu Pro Gly Val Asp Ala Tyr Val Trp Val 705 710 715 720 Lys Pro Gln Gly Glu Ser Asp Gly Ile Ser Asp Pro Asn Phe Pro Ile                 725 730 735 Asp Pro Asn Asp Pro Ala Lys Gln His Asp Pro Met Cys Asp Pro Asn             740 745 750 Ala Pro Asn Arg Asp Asn Asn Ala Val Gly Thr Gly Ala Leu Asp Asn         755 760 765 Ala Pro His Ala Gly Arg Trp Phe Pro Glu Ala Phe Gln Ile Leu Ile     770 775 780 Glu Asn Ala Tyr Pro Pro Leu 785 790 <210> 25 <211> 65 <212> PRT <213> Microbulbifer degradans <400> 25 Met Pro Leu Glu Pro Asp Asp Glu Leu Asp Glu Glu Val Leu Glu Glu 1 5 10 15 Leu Asp Glu Glu Leu Leu Val Leu Leu Glu Leu Leu Glu Glu Leu Asp             20 25 30 Glu Leu Asp Asp Glu Leu Glu Leu Glu Glu Leu Glu Pro Leu Ser His         35 40 45 Thr Ala Pro Val Thr Val Gly Ile Ser Ala Leu Glu Pro Ala Leu Leu     50 55 60 Pro 65 <210> 26 <211> 112 <212> PRT <213> Microbulbifer degradans <400> 26 Met Asn Gly Leu Ser Lys Pro Ala Pro Leu Pro Glu Val Glu Glu Leu 1 5 10 15 Glu Leu Glu Glu Asp Glu Leu Glu Leu Asp Asp Val Leu Glu Leu Glu             20 25 30 Leu Glu Leu Glu Leu Val Glu Leu Val Leu Glu Leu Glu Leu Glu Glu         35 40 45 Val Leu Glu Val Glu Glu Leu Leu Glu Leu Glu Val Glu Pro Val Glu     50 55 60 Glu Leu Glu Leu Glu Leu Glu Val Leu Glu Pro Asp Glu Leu Asp Glu 65 70 75 80 Leu Leu Asp Glu Leu Val Leu Glu Pro Glu Leu Asp Glu Pro Glu Leu                 85 90 95 Glu Ala Pro Leu Gln Val Thr Pro Pro Lys Glu Pro Ser Trp Phe Pro             100 105 110 <210> 27 <211> 2376 <212> DNA <213> Microbulbifer degradans <400> 27 atgttggctt ctaataaaaa tagtaagctg gcaaactctg agcaacaccg cccttataaa 60 acccgcacag cgcgctggtt aaccgggtct ggggttattg cttcaagttt gcttttttct 120 gcgcagagtt ttgcggcgca atgtgaatac atcattagca atgaatggaa cagcggcttt 180 actggcgcag ttcgcattac taataatggc actactccca tcaatggctg ggatgttagc 240 tggcagtatg ccggcgatgc agtcaccagc agctggaacg cgaatgtttc tggctcgaac 300 cccgtttctg ctacaccatt aagctggaat gccaacattc aacccggtca aagcgttgag 360 tttggttttc agggcagcaa agccggctcc aatgcagaaa ttccaaccgt taccggcgcg 420 gtatgtgata gcggctctag ctcttccagc tccagctcat catctagttc atcaagctct 480 tctagtagct caagcagcac tagcagctcc tcgtccagct cttcaagcac ctcttcgtct 540 agctcatcat ctggctccag tggcacaggt ggtattgcgt gtactgtagg caatgcgaat 600 atttggggct cgggctacca gctggacatg caagttgtta acaacggcac cgctgcagta 660 agcagttggg acgtaaccat ggcattcggc gaggcaccac agcgcaccgg tggctggaac 720 gcaaactttg tagagtcagg caataccatt gttgcgagca acattagctg gaacggcaac 780 ctcgcaccgg ggcaatcagc ttcgtttggt attcaaggga accacgacgg ctcttttggc 840 ggcgtaacct gtaacggcgc ttcaagctct ggctcgtcta gttctggctc tagcaccagc 900 tcatcaagta gctcatccag ttcgtctggc tctagcactt ctagctctag ctcaagctcc 960 tctactggtt ctacctctag ctctagtagc tcttcaactt ctagcacttc ttcaagttct 1020 agctctagca ccagctccac gagttctagc tccagttcta gctcgagtac atcgtctagt 1080 tccagctcat cttcctcaag ctctagctct tctacttcag gcagtggcgc aggttttgac 1140 aacccgttca ttggcggcaa gtggtatgta gacccagtat ggtcagcaaa agctgcagca 1200 gagccaaacg gttcacttat tgccaactac aacacggcag tttggatgga tcgcattggt 1260 gcgattgaag gcccagaaga tggcgatggt atgggcttag aagaacactt agatgaagct 1320 ttagcacaag gtgcagacat ctttatgttc gtggtatacg acctaccaaa ccgcgactgt 1380 gcagctttgg cctcaagtgg cgaactactc attgccgaga acggttttga gcgctatcaa 1440 aatgagtaca ttggcccaat cgtagatata ctcagcaagc ccgcgtattc tagcttgcgt 1500 attatcgcga ttattgaagt ggattctcta cccaacctcg ttaccaacct caacattcaa 1560 aaatgtgttg aagcgaatgg cccgggtggg tacgtagacg gtatccaaca tgcacttaac 1620 gagctaaaca cgcttgataa tgtgtaccca tacgtcgata ttgctcactc aggctggcta 1680 ggctggagcg acaacttcgc cggcgccacc aagcttattg gtgatgcaat taaaggcaca 1740 aacaaaggtg taaacagtat tgcaggcttt gtaagtaact cttctaacta cacacctgtg 1800 actgaaccat acctacctaa ccctaccttg caaattggta gcaaccaagt tcgatctgcg 1860 gatttctacg agtggaccat gtacttcgaa gaacttagct ttgtacaaga ttggcgccaa 1920 gccatgattc agcaaggctt cccagaatca attggtatgc ttattgatac cgcacgtaat 1980 ggctggggtg gacctgaccg tccaactggt gagtctacat ctaccgacct caacacctat 2040 gtgaatgaat cgcgtataga ccgccgtcag catcgcggaa actggtgtaa ccagcccggt 2100 ggtgttggct tccgtccgca agcggcacca gaaccaggtg tagacgctta cgtttgggtt 2160 aagccacaag gtgagtcgga tggtattagt gatcctaact tccctatcga ccctaacgac 2220 ccagctaaac agcacgaccc aatgtgtgat ccaaacgcac ctaaccgcga taacaatgcg 2280 gttggcacag gcgcgctaga taacgctcca catgctggtc gctggttccc agaagcattc 2340 caaatactta tagaaaacgc ctacccaccg ctatag 2376 <210> 28 <211> 578 <212> PRT <213> Microbulbifer degradans <400> 28 Met Asn Lys Val Lys Val Leu Ala Leu Cys Ala Ser Val Ala Val Met 1 5 10 15 Ile Gly Cys Ser Asp Ala Asp Thr Lys Leu Ala Asn Ser Ala Lys Ala             20 25 30 Glu Val Gly Phe Thr Lys Val Asn Gln Leu Gly Tyr Leu Pro Ala Ala         35 40 45 Lys Lys Leu Ala Val Val Pro Ala Val Ala Ala Ala Lys Phe Asp Ile     50 55 60 Ile Asp Val Thr Ser Gly Lys Val Ala Phe Thr Gly Ser Leu Ser Asp 65 70 75 80 Val Lys Ser Trp Ser Ala Met Gly Asp Glu Ser Phe Lys Leu Ala Asp                 85 90 95 Phe Ser Ala Leu Gln Ala Glu Gly Ser Tyr Arg Leu Val Val Gln Gly             100 105 110 Val Ser Asp Ser Tyr Thr Phe Asp Ile Ser Pro Ser Val Tyr Ser Gln         115 120 125 Ala His Asp Gly Ala Leu Lys Ala Tyr Tyr Tyr Asn Arg Ala Ser Thr     130 135 140 Glu Leu Thr Glu Gln Tyr Ala Gly Val Tyr Ala Arg Pro Ala Gly His 145 150 155 160 Pro Asp Thr Asp Val Arg Ile Phe Asp Asn Ala Ala Ser Ala Ala Arg                 165 170 175 Pro Ala Asp Thr Ser Phe Ala Ala Pro Lys Gly Trp Tyr Asp Ala Gly             180 185 190 Asp Tyr Gly Lys Tyr Ile Val Asn Ser Gly Ile Ser Thr Tyr Thr Leu         195 200 205 Met Ala Ala Tyr Glu His Phe Pro Ser Phe Tyr Lys Gln Arg Asp Ile     210 215 220 Asp Ile Pro Glu Ser Gly Asp Ala Val Pro Asp Ile Leu Asp Glu Val 225 230 235 240 Met Trp Asn Leu Glu Trp Met Gln Val Met Gln Asp Pro Asn Asp Gly                 245 250 255 Gly Val Tyr His Lys Leu Thr Thr Leu Asn Phe Ser Gly Ala Val Met             260 265 270 Pro His Glu Ala Thr Ala Gln Arg Tyr Phe Ile Lys Lys Ser Thr Ala         275 280 285 Ala Thr Leu Asp Phe Ala Ala Val Met Ala Thr Ala Ser Arg Val Tyr     290 295 300 Ala Pro Phe Glu Gly Ala Phe Pro Gly Lys Ser Ala Ala Tyr Arg Gln 305 310 315 320 Ala Ala Ile Ala Ala Trp Glu Trp Ala Gln Ala Asn Pro Ser Glu Thr                 325 330 335 Tyr Ser Gln Thr Pro Leu Ser Lys Val Gln Thr Gly Ala Tyr Gly Asp             340 345 350 Lys Lys Leu Asn Asp Glu Phe Ala Trp Ala Ala Ala Glu Leu Phe Ile         355 360 365 Leu Thr Gly Glu Gln Lys Tyr Trp Gln Ala Phe Asn Lys Gln Lys Val     370 375 380 Gln Ala Gly Glu Ser Ser Trp Ala Asn Val Ala Gly Leu Gly Phe Ile 385 390 395 400 Ser Leu Ala Asn Asn Ala Arg Ser Leu Leu Asn Glu Ala Gln Tyr Lys                 405 410 415 Thr Val Thr Asp Ser Ile Val Arg Ala Ala Asp Ser Leu Leu Val Thr             420 425 430 Tyr Lys Glu Asn Ala Tyr Gln Val Pro Ile Gly Asn Lys Asp Phe Phe         435 440 445 Trp Gly Gly Asn Ser Gly Thr Leu Asn Arg Ala Trp Val Leu Leu Glu     450 455 460 Ala Asn Lys Ile Lys Pro Gln Gln Glu Tyr Ile Asp Ala Ala Leu Ala 465 470 475 480 Ala Val Asp Tyr Ile Tyr Gly Arg Asn Pro Thr Asn Tyr Ser Phe Val                 485 490 495 Thr Gly Phe Gly Asp Asn Pro Ala Val Gly Ile His His Arg Pro Ser             500 505 510 Tyr Ala Asp Gly Ile Lys Ala Pro Val Pro Gly Trp Leu Ala Gly Gly         515 520 525 Ala His Asn Gly Lys Gln Asp Gly Cys Glu Tyr Pro Ser Asp Ala Pro     530 535 540 Ala Lys Ser Tyr Leu Asp Asp Trp Cys Ser Tyr Ser Thr Asn Glu Ile 545 550 555 560 Ala Ile Asn Trp Asn Ala Pro Leu Val Tyr Ile Leu Ala Ala Val Asn                 565 570 575 Asn leu          <210> 29 <211> 1737 <212> DNA <213> Microbulbifer degradans <400> 29 atgaacaaag ttaaagtttt agcgctgtgt gccagtgtgg ctgtaatgat aggttgcagt 60 gatgccgaca ctaaattagc taactcggcc aaggccgagg tgggctttac caaagtgaat 120 cagctgggtt atttgcccgc ggccaaaaag ctggcggtgg tacccgccgt tgcagctgca 180 aaattcgaca taatcgatgt aactagcggt aaagtagcgt ttacggggag tttaagcgac 240 gtaaaaagct ggagcgcgat gggggacgaa tctttcaagt tggcagactt tagcgccctg 300 caagccgaag ggagttaccg cttagttgtt cagggtgtga gtgattctta caccttcgat 360 attagcccaa gtgtatatag ccaagcgcac gatggagccc ttaaagccta ttactataat 420 cgagcgagca cagagttaac agaacagtac gccggggtgt atgcgcgacc tgcggggcac 480 ccagataccg acgtacgcat attcgataac gccgcctcag ccgcgcgccc agcagataca 540 agctttgctg caccaaaggg ttggtacgat gctggcgatt acggcaagta cattgttaac 600 agtggtattt ccacttacac cctaatggct gcgtacgagc atttcccgtc gttttacaag 660 caacgcgata tagatattcc cgaatctggc gatgccgtac cggatattct cgacgaggta 720 atgtggaacc ttgaatggat gcaggtcatg caagacccga acgacggcgg tgtgtaccac 780 aagcttacca ccctgaattt ttctggcgca gtcatgccgc acgaagcgac tgcgcagcgc 840 tattttatta aaaaatctac cgctgcaacg ctagattttg ccgcggttat ggccactgca 900 agccgagtat acgcaccgtt cgaaggtgct tttcctggta aatcagctgc ttatcgacag 960 gcggccattg ctgcgtggga gtgggcacaa gcaaacccta gtgagacata ttcgcagaca 1020 ccgctgagca aagttcaaac cggcgcctat ggtgataaaa agttaaacga tgaatttgcg 1080 tgggcggccg cagagttgtt tatattgacc ggcgagcaaa aatactggca ggcgtttaac 1140 aagcaaaaag tgcaggcggg tgagtctagc tgggcgaatg ttgcggggtt ggggtttatt 1200 tccttggcca ataatgcgcg cagcctgtta aacgaagctc aatacaaaac cgttaccgat 1260 tcaattgttc gcgctgcaga tagcttgctt gttacttaca aagagaatgc ctaccaagta 1320 cccattggca acaaagattt tttctggggt ggcaattccg gcacgttaaa tcgcgcttgg 1380 gttttgcttg aggccaataa aattaaaccg cagcaagaat acatcgatgc tgcacttgcc 1440 gcggtggatt atatttatgg tcgcaaccct accaactact cttttgtcac tgggtttggc 1500 gataaccctg cggtgggtat ccatcatcgt ccatcctatg ccgatggcat taaagcccct 1560 gtgcctggtt ggcttgcggg cggtgcgcac aatggcaagc aagatggttg tgagtaccct 1620 tccgatgcac cggcaaaatc ctatctagac gactggtgca gttactccac caacgaaatt 1680 gctattaatt ggaatgcgcc gttagtttac atactggctg cggtaaataa tttgtag 1737 <210> 30 <211> 867 <212> PRT <213> Microbulbifer degradans <400> 30 Met Asn Leu Thr Ser Ile Met Phe Glu Gln Ser Val Lys Lys Val Ala 1 5 10 15 Lys Ser Ala Ile Ala Val Ala Val Ala Ser Ala Val Thr Leu Ser Ala             20 25 30 Ala Gln Ala Glu Val Gly Asn Pro Arg Val Asn Gln Val Gly Tyr Ile         35 40 45 Pro Asn Gly Ala Lys Val Ala Ser Tyr Val Ala Pro Ser Asn Thr Ala     50 55 60 Gln Thr Trp Gln Leu Leu Arg Asn Gly Ser Val Val Ala Ser Gly Thr 65 70 75 80 Thr Thr Pro Lys Gly Thr Asp Ala Ala Ser Gly Asp Asn Ile His His                 85 90 95 Ile Asp Phe Ser Ala Val Ser Ala Thr Gly Glu Gly Phe Ser Leu Leu             100 105 110 Val Gly Gly Asp Glu Ser Tyr Pro Phe Glu Ile Ser Ala Asp Ala Phe         115 120 125 Thr Pro Val Leu Tyr Asp Ser Ile Arg Tyr Phe Tyr His Asn Arg Ser     130 135 140 Gly Ile Ala Ile Glu Thr Gln Tyr Thr Gly Gly Gly Asn Gly Ser Tyr 145 150 155 160 Ala Ala Asn Ala Gln Trp Ala Arg Pro Ala Gly His Ile Asn Gln Asn                 165 170 175 Ala Asn Gln Gly Asp Asn Ala Val Pro Cys Trp Ser Gly Ser Gly Cys             180 185 190 Asn Tyr Ala Leu Asp Val Thr Lys Gly Trp Tyr Asp Ala Gly Asp His         195 200 205 Gly Lys Tyr Val Val Asn Gly Gly Ile Ser Val Trp Lys Leu Leu Asn     210 215 220 Met Tyr Glu Arg Ala Leu His Ile Ser Gly Ser Gln Asn Lys Tyr Ala 225 230 235 240 Asp Gly Thr Leu Asn Ile Pro Glu Ser Gly Asn Gly Val Ala Asp Ile                 245 250 255 Leu Asp Glu Ala Arg Trp Gln Met Glu Phe Leu Leu Ala Met Gln Val             260 265 270 Pro Glu Gly Glu Ala Lys Ala Gly Met Val His His Lys Met His Asp         275 280 285 Val Gly Trp Thr Gly Leu Pro Leu Ala Pro His Glu Asp Asn Arg Glu     290 295 300 Arg Ala Leu Val Pro Pro Ser Val Thr Ala Thr Leu Asn Val Ala Ala 305 310 315 320 Thr Gly Ala Gln Cys Ala Arg Leu Phe Asp Glu Ile Asp Ala Ser Phe                 325 330 335 Ala Ala Ser Cys Leu Thr Ala Ala Glu Arg Ala Trp Asp Ala Ala Leu             340 345 350 Gln Asn Pro Asn Asp Val Tyr Thr Gly Gly Tyr Asp Asn Gly Gly Gly         355 360 365 Gly Tyr Gly Asp Glu Val Ala Asp Asp Glu Phe Phe Trp Ala Ala Ala     370 375 380 Glu Leu Tyr Ile Thr Thr Gly Asp Ser Lys Tyr Leu Ser Thr Ile Asn 385 390 395 400 Asn Tyr Asn Val Thr Arg Ile Asp Trp Gly Trp Pro Asp Thr Glu Leu                 405 410 415 Pro Ala Leu Met Ser Leu Ala Val Val Pro Ala Asn His Thr Ala Asn             420 425 430 Leu Arg Ala Thr Ala Arg Ala Lys Ile Val Glu Ile Ala Asp Thr His         435 440 445 Val Ala Thr Ser Asn Ala Ala Gly Tyr Leu Thr Pro Ser Ser Ala Leu     450 455 460 Asp Tyr Tyr Trp Gly Ser Asn Asn Gly Val Ala Asn Lys Ile Ala Leu 465 470 475 480 Leu Gly Leu Ala Tyr Asp Phe Thr Gly Asp Asp Val Tyr Ala Lys Thr                 485 490 495 Val Ser Lys Ala Val Asn Tyr Leu Phe Gly Asn Asn Thr Leu Ser Phe             500 505 510 Ser Tyr Ile Ser Gly His Gly Glu Asn Ala Leu Gln Gln Pro His His         515 520 525 Arg Phe Trp Ala Gly Ala Leu Asn Gly Ser Tyr Pro Trp Leu Pro Pro     530 535 540 Gly Ala Leu Ser Gly Gly Pro Asn Ala Gly Leu Glu Asp Gly Val Ala 545 550 555 560 Ala Ala Ala Leu Ser Ala Cys Val Ser Thr Pro Ala Lys Cys Tyr Met                 565 570 575 Asp Asp Ile Glu Ser Trp Ser Thr Asn Glu Ile Thr Ile Asn Trp Asn             580 585 590 Gly Ala Leu Val Trp Ala Met Ala Phe Tyr Asp Asp Tyr Ala Asp Ser         595 600 605 Gly Ser Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser     610 615 620 Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser 625 630 635 640 Ser Ser Ser Ser Gly Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser                 645 650 655 Ser Ser Ser Ser Ser Ser Ser Gly Gly Glu Cys Val Glu Met Cys Lys             660 665 670 Trp Tyr Gln Asp Ala Pro Arg Pro Leu Cys Asn Asn Gln Asn Ser Gly         675 680 685 Trp Gly Trp Glu Asn Gln Gln Ser Cys Ile Gly Arg Thr Thr Cys Glu     690 695 700 Ser Gln Ser Gly Asn Gly Gly Val Ile Asn Ser Cys Gly Thr Ser Ser 705 710 715 720 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser                 725 730 735 Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser             740 745 750 Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Gly Gly         755 760 765 Val Ala Gly Val Ala Cys Ala Val Thr Lys Met Asn His Trp Gly Ser     770 775 780 Gly Tyr Gln Leu Asp Val Thr Val Ser Asn Asn Gly Ala Ala Ala Val 785 790 795 800 Ser Gly Trp Ser Ile Glu Leu Asp Phe Gly Glu Ser Pro Gln Leu Thr                 805 810 815 Gly Ser Trp Asn Ala Ala Val Ser Ala Ser Gly Asn Thr Val Ser Ala             820 825 830 Thr Asn Ile Ser Trp Asn Gly Asn Leu Ser Ala Gly Gln Ser Thr Ser         835 840 845 Phe Gly Met Gln Gly Asn Ser Asp Gly Ser Leu Ser Thr Pro Ser Cys     850 855 860 Leu Val Lys 865 <210> 31 <211> 49 <212> PRT <213> Microbulbifer degradans <400> 31 Met Glu Glu Leu Leu Glu Glu Leu Glu Pro Leu Asp Glu Glu Leu Leu 1 5 10 15 Leu Glu Asp Asp Glu Leu Glu Val Glu Leu Glu Glu Leu Asp Glu Glu             20 25 30 Leu Asp Glu Leu Glu Glu Leu Asp Glu Leu Glu Pro Leu Pro Glu Ser         35 40 45 Ala      <210> 32 <211> 2604 <212> DNA <213> Microbulbifer degradans <400> 32 atgaatctta cttcaatcat gtttgaacaa tcagtaaaaa aagtcgctaa gtcagccatt 60 gccgtggcag ttgcttcggc ggttacctta agtgcggcgc aggccgaggt gggtaaccca 120 cgtgttaacc aagtaggcta tatacccaat ggtgccaaag ttgccagtta tgttgcgcca 180 tcaaatacgg cacaaacgtg gcagttactg cgtaatggca gtgtggttgc aagtggcact 240 acaaccccaa agggtacaga tgcagcctcg ggtgacaata ttcaccatat cgatttttct 300 gcggtgagtg caaccggcga aggttttagt ttgcttgtgg gcggcgatga aagttacccc 360 tttgaaattt ctgccgacgc atttacaccg gttttatacg attccatccg ttacttttat 420 cacaaccgtt cgggtatcgc gattgaaacg cagtacaccg gtggcggtaa cggtagctac 480 gcggcgaatg ctcagtgggc taggcccgca ggtcacatta atcaaaatgc taaccaaggc 540 gataatgcgg tgccgtgttg gtcgggcagt ggttgcaact acgccttaga cgtaactaaa 600 ggttggtacg atgccggtga ccacggtaaa tatgttgtaa acggtggcat ttccgtatgg 660 aagctattaa acatgtacga gcgtgccttg cacattagtg gcagccaaaa taaatacgcc 720 gacggtacat taaatattcc tgaaagcggc aatggcgtgg cggatatttt ggatgaagct 780 cgctggcaaa tggagttttt attagccatg caagtgccag agggcgaagc gaaagctggc 840 atggtgcacc acaaaatgca cgatgtgggt tggacaggct tgccactagc accccatgaa 900 gataatcgcg agcgcgcgct tgtgccgcct tcggttactg caacccttaa cgttgcggcc 960 acaggcgcgc agtgtgcgcg tttatttgac gaaatagatg cgagttttgc agcaagttgt 1020 ttaactgccg cagagcgcgc atgggatgca gccctgcaaa accctaacga tgtttacact 1080 ggcggctacg ataatggcgg cggtggttac ggcgatgaag tggcggacga cgagtttttc 1140 tgggctgctg ctgagttata cattaccact ggcgatagca aatatctttc aaccattaac 1200 aactacaatg taacgcgcat tgattggggc tggccagata ccgagttgcc tgcgttgatg 1260 tcgttagcgg ttgtgcctgc taatcacacc gcaaatttgc gtgcgactgc tcgtgcaaaa 1320 attgtagaaa ttgcagatac ccatgtcgct accagtaatg ctgccggcta tttaacacca 1380 tcgtccgcgc tggattacta ctggggttct aacaatggcg tagccaataa aattgcgtta 1440 cttggtttgg catacgattt tactggcgat gacgtttacg cgaaaacggt gtcgaaagca 1500 gttaactatt tatttggtaa taatacctta tcgttttctt atatttctgg gcatggcgaa 1560 aatgctttgc aacagccgca tcaccgcttt tgggctgggg cattaaatgg aagttaccca 1620 tggttgccgc ctggtgcgct ttctggtggc cctaacgcag ggttagaaga tggcgttgcc 1680 gccgccgcgc taagtgcttg tgtttcaacg cctgccaaat gctatatgga tgatattgaa 1740 tcttggtcga ccaacgaaat tactattaac tggaatggtg cattggtttg ggcaatggcg 1800 ttttatgatg actacgccga ttcgggtagc ggttctagct cgtcaagttc ttctagctca 1860 tctagctctt cgtcaagttc ttccagttcg acttctagct cgtcgtcttc tagtagtagc 1920 tcttcgtcga gcggctcgag ttcttctagc agctcttcca catccagttc cagctcttcg 1980 agttcatcgg gtggggagtg tgtagaaatg tgtaagtggt atcaagatgc accgcgccct 2040 ctatgcaata accaaaacag cggttgggga tgggagaacc agcagagttg tattggtaga 2100 acaacttgcg aaagtcaaag tggcaatggt ggagtgatta attcgtgcgg cacgtctagc 2160 tcgagctctt catctagctc tagcagtagc tcttcgagtt catccagctc ttctagcagt 2220 tcttccacat caagctcgtc gagtagttcg tcttctagct cttctagttc gacttcaagt 2280 tcttcgtcga gcagttcagg gggcgttgca ggtgtggctt gtgcggtaac caaaatgaac 2340 cattggggca gcggatatca attagatgta acagtttcta ataatggtgc tgcagcggta 2400 agtggttgga gtattgaact cgattttggt gaatcgccac agcttactgg tagttggaat 2460 gctgctgtat cggcatctgg taatactgta tcggctacta acattagttg gaacggtaat 2520 ttaagcgctg ggcaatctac ctcttttggt atgcagggta attcagatgg ttcgctgagc 2580 acgccaagct gtttagttaa gtaa 2604 <210> 33 <211> 1072 <212> PRT <213> Microbulbifer degradans <400> 33 Met Lys Asn Thr Leu Ser Phe Lys Thr Ser Leu Leu Ala Gly Leu Val   1 5 10 15 Ala Ser Ser Leu Leu Val Ala Ala Cys Gln Gly Val Lys Gln Gln Thr              20 25 30 Glu Ala Thr Gln Thr Lys His Asn Ile Thr Leu Trp Pro Gln Ala Ser          35 40 45 Ser Pro Val Ile Lys Ser Pro Asp Tyr Glu Ala Glu Val Glu Ala Lys      50 55 60 Val Glu Ala Leu Leu Gly Gln Met Thr Leu Glu Gln Lys Val Gly Gln  65 70 75 80 Ile Leu Gln Pro Glu Ile Gln Ser Ile Lys Pro His Glu Val Lys Glu                  85 90 95 Tyr His Ile Gly Ser Val Leu Asn Gly Gly Gly Ser Met Pro Asn Arg             100 105 110 Ile Glu Asn Ala Pro Pro Ile Glu Trp Val Lys Leu Ala Asp Ala Phe         115 120 125 Tyr Asp Ala Ser Met Asp Asp Ser Asp Gly Gly Ile Ala Ile Pro Ile     130 135 140 Ile Trp Gly Thr Asp Ala Val His Gly His Gly Asn Val Thr Gly Ala 145 150 155 160 Thr Ile Phe Pro His Asn Ile Gly Leu Gly Ala Ala Arg Asn Pro Ala                 165 170 175 Leu Ile Glu Lys Ile Gly Glu Ile Thr Ala Lys Glu Val Arg Ala Thr             180 185 190 Gly Ile Glu Trp Ile Phe Gly Pro Thr Leu Ala Val Ala Gln Asn Asp         195 200 205 Leu Trp Gly Arg Thr Tyr Glu Ser Tyr Ser Glu Asp Pro Ala Ile Val     210 215 220 Ala Asp Tyr Ala Ser Ala Met Val Val Gly Met Gln Gly Lys Val Asp 225 230 235 240 Asp Ser Asp Phe Leu Ser Thr Asn Arg Val Val Ala Thr Ala Lys His                 245 250 255 Phe Leu Ala Asp Gly Gly Thr Leu Gly Gly Asn Asp Gln Gly Asp Ala             260 265 270 Arg Ile Ser Glu Glu Glu Leu Val Gln Ile His Asn Ala Gly Tyr Val         275 280 285 Pro Ala Ile Glu Ser Gly Val Gln Thr Val Met Ala Ser Phe Ser Leu     290 295 300 Trp Asn Gly Val Lys Met His Gly Asn Asn Tyr Leu Leu Thr Gln Ala 305 310 315 320 Leu Lys Glu Arg Met Gly Phe Asp Gly Phe Ile Val Gly Asp Trp Asn                 325 330 335 Gly His Gly Gln Val Pro Gly Cys Thr Asn Glu Ser Cys Pro Gln Ser             340 345 350 Leu Asn Ala Gly Leu Asp Met Tyr Met Val Pro Tyr Asp Trp Lys Lys         355 360 365 Leu Tyr Arg Asn Leu Ile Ser Gln Val Gln Ser Gly Glu Ile Ala Pro     370 375 380 Ser Arg Leu Asp Asp Ala Val Arg Arg Ile Leu Arg Val Lys Ile Arg 385 390 395 400 Ala Asn Leu Trp Ala Ala Lys Pro Ser Glu Arg Ile Asn Leu Ala Thr                 405 410 415 Ile Asp Glu Val Val Gly His Ala Asn His Arg Glu Val Ala Arg Gln             420 425 430 Ala Val Arg Glu Ser Leu Val Leu Leu Lys Asn Lys Asn Ser Val Leu         435 440 445 Pro Ile Ala Ala Asn Lys Thr Val Leu Val Ala Gly Asp Gly Ala Asp     450 455 460 Asn Ile Gly Lys Gln Ser Gly Gly Trp Ser Val Ser Trp Gln Gly Thr 465 470 475 480 Gly Asn Thr Asn Ala Ser Phe Pro Gly Gly Thr Ser Ile Tyr Lys Gly                 485 490 495 Ile Ala Asp Ala Val Thr Gln Gly Gly Gly Lys Ala Thr Leu Ser Val             500 505 510 Asp Gly Ser Tyr Lys Thr Lys Pro Asp Val Ala Ile Val Val Ile Gly         515 520 525 Glu Asp Pro Tyr Ala Glu Gly Gln Gly Asp Arg Asn Ser Leu Glu Phe     530 535 540 Glu Pro Val Asn Lys Lys Ser Leu Glu Leu Leu Lys Lys Leu Lys Ala 545 550 555 560 Asp Gly Ile Pro Val Val Thr Val Phe Ile Ser Gly Arg Pro Met Trp                 565 570 575 Ala Asn Pro Glu Ile Asn Ala Ser Asp Ala Phe Val Ala Ala Trp Leu             580 585 590 Pro Gly Ser Glu Gly Gln Gly Val Ala Asp Val Leu Ile Gly Asn Ala         595 600 605 Asn Gly Lys Pro Arg Phe Asp Phe Lys Gly Thr Leu Ser Phe Ser Trp     610 615 620 Pro Lys Leu Pro Thr Gln Gly Leu Leu Asn Pro Thr His Pro Asn Tyr 625 630 635 640 Asp Pro Leu Phe Lys Leu Gly Tyr Gly Leu Thr Tyr Ala Ser Ser Glu                 645 650 655 Thr Gly Pro Glu Gln Leu Ala Glu Asp Val Glu Gly Val Asp Lys Gly             660 665 670 Ser Thr Gly Asp Ile Asn Phe Tyr Val Gly Arg Thr Leu Glu Pro Trp         675 680 685 Glu Val Phe Val Arg Thr Pro Glu Ser Ser Gln Arg Leu Ser Gly Pro     690 695 700 Phe Ala Asp Leu Gly Asn Ala Ser Val Arg Thr Ser Asp Met Gln Val 705 710 715 720 Gln Glu Asp Ala Leu Thr Phe Thr Trp Gly Gly Ser Trp Met Ser Ile                 725 730 735 Leu Gly Ile Glu Gly Gly Arg Gly Tyr Asp Leu Ser Ser Gln Tyr Lys             740 745 750 Glu Gly Gly Val Ile Ser Phe Asn Phe Asn Ser Ile Asp Met Ala Lys         755 760 765 Gly Asp Leu Lys Val Gln Met Ala Cys Gly Glu Gly Cys Thr Arg Glu     770 775 780 Val Asp Ile Thr Thr Ile Ala Arg Asp Leu Glu Gly Lys Gly Trp Gln 785 790 795 800 Ser Leu Thr Val Pro Leu Ala Cys Phe Ala His Glu Gly Asp Asp Phe                 805 810 815 Thr His Ile Thr Ala Pro Phe Asn Leu Phe Ala Gly Gly Lys Gly Gln             820 825 830 Val Ala Val Ala Asn Ile Arg Ile Leu Arg Ala Gly Thr Gln Thr Val         835 840 845 Pro Cys Val Leu Pro Lys Asp Val Ser Val Thr Pro Glu Pro Leu Asn     850 855 860 Ala Ser Trp Ala Ile Asp Trp Trp Met Pro Arg His Lys Glu Lys Leu 865 870 875 880 Ala Arg Ile Gln Gln Gly Asn Val Asp Leu Leu Met Ile Gly Asp Ser                 885 890 895 Ile Thr His Gly Trp Glu Asp Ala Gly Lys Asp Val Trp Ala Gln Tyr             900 905 910 Tyr Ala His Arg Asn Ala Val Asp Leu Gly Phe Ser Gly Asp Arg Thr         915 920 925 Glu Asn Val Leu Trp Arg Leu Gln His Gly Glu Ala Asp Gly Ile Lys     930 935 940 Pro Lys Val Ala Val Val Met Ile Gly Thr Asn Asn Ala Gly His Arg 945 950 955 960 His Glu Pro Ser His Tyr Thr Ala Lys Gly Val Ala Ala Val Val Ala                 965 970 975 Glu Leu Gln Lys Arg Leu Pro Glu Thr Lys Ile Leu Leu Leu Gly Ile             980 985 990 Phe Pro Arg Gly Glu Thr Ser Glu Asp Pro Leu Arg Val Leu Asn Ala         995 1000 1005 Lys Thr Asn Thr Leu Leu Ala Lys Met Ala Asp Gly Glu Lys Val Val    1010 1015 1020 Tyr Leu Asn Ile Asn Lys Thr Phe Leu Asp Glu Asn Gly Val Leu Pro 1025 1030 1035 1040 Lys Asp Ile Met Pro Asp Leu Leu His Pro Asn Glu Lys Gly Tyr Ala                1045 1050 1055 Leu Trp Ala Lys Ala Met Glu Pro Thr Leu Lys Lys Met Leu Gly Glu            1060 1065 1070 <210> 34 <211> 3219 <212> DNA <213> Microbulbifer degradans <400> 34 atgaaaaata ctttatcctt taaaacatcc ttgcttgcgg gcttggtggc atccagttta 60 ctggttgcgg cctgtcaggg tgttaaacag caaacggaag ctactcagac aaagcacaat 120 attaccttat ggccgcaggc gtctagccct gtaataaagt cgccagatta cgaagcggaa 180 gtggaagcca aggtagaagc gttgttagga caaatgacgc tagagcaaaa agtagggcaa 240 atcctacagc cagaaattca atctattaag ccgcatgaag taaaagaata ccacattggc 300 tctgtactaa atggtggtgg ctctatgcct aaccgcatag aaaatgcgcc gcccattgaa 360 tgggtaaaat tggccgatgc cttttacgat gcctctatgg acgattctga cggtggaatc 420 gcaattccca ttatttgggg taccgatgcc gtacacggtc acggcaatgt aactggcgca 480 accatattcc cgcataacat aggccttggt gctgcacgca acccagcgct tatcgaaaaa 540 attggcgaaa taacggcaaa agaagtacgc gcaaccggca ttgaatggat atttggccca 600 actttggccg tagcgcaaaa cgatttatgg ggccgcactt acgaaagcta ctcggaagac 660 ccagccatag tggccgacta cgccagtgcc atggtggtag gtatgcaggg caaagtggac 720 gacagcgatt ttctgtccac taatcgcgta gttgccacag caaagcactt tttagctgac 780 ggcggtacct taggaggcaa cgatcaaggt gatgcgcgca taagcgaaga agagttggtg 840 caaattcata atgcgggcta tgtgcctgcc attgaatcgg gcgtgcaaac ggttatggcc 900 agtttctctt tgtggaatgg cgtaaaaatg catggtaaca actacctact tacccaagca 960 cttaaagagc gtatggggtt tgatggtttt atagtagggg attggaatgg ccacgggcag 1020 gtacctgggt gcaccaacga atcttgccct caatcgctaa acgccggttt agatatgtac 1080 atggtgcctt acgattggaa aaaactgtac agaaacttaa ttagccaagt gcaatcgggt 1140 gaaattgccc caagccgttt agatgacgct gtacgccgta ttcttcgggt aaaaattcgc 1200 gctaatttgt gggctgcgaa accttcagag cgaattaatc tagccactat tgacgaggtg 1260 gttggccacg caaaccaccg tgaggtagcg cggcaggcgg tgcgagaaag tttagtattg 1320 ttaaaaaata aaaatagcgt actgcctatt gctgccaata aaaccgtgct ggttgcaggt 1380 gacggcgccg ataatattgg caaacaatct ggcggttgga gtgtaagctg gcagggcact 1440 ggtaacacca atgcatcctt ccccggtggt acatctattt ataaaggtat tgccgatgca 1500 gtcactcagg gcggcggtaa agctacgctt tctgtggatg gcagctacaa aactaaaccc 1560 gatgttgcca ttgtggtaat aggcgaagac ccttacgccg aaggccaagg cgaccgcaat 1620 agtttagagt tcgagccggt gaataaaaaa tcgcttgagc tattaaaaaa attaaaagca 1680 gatggcatac ccgttgtaac agtatttatt tctggccgac ctatgtgggc taacccagaa 1740 attaacgcgt ctgatgcatt tgttgccgcg tggttacctg gctctgaagg gcagggcgta 1800 gcagatgtac ttataggcaa cgccaacggc aagcctcgtt ttgatttcaa gggcaccttg 1860 tcgttctctt ggcctaagct gccgacccaa ggcttgctca acccaacgca ccccaactac 1920 gacccgttat ttaaattggg atacgggcta acttatgcct cgagtgaaac tggcccagag 1980 caattggcgg aagatgttga aggtgtagat aaaggctcaa ccggcgacat taatttttat 2040 gttggccgca cattagagcc gtgggaagtg tttgttcgaa ctcctgaaag ttcgcagcgt 2100 ttaagtggcc catttgcaga cttaggcaat gccagtgtgc gtaccagtga tatgcaggta 2160 caagaagatg cccttacttt tacttggggc ggtagctgga tgtctattct gggaatagaa 2220 ggagggcgcg gttacgacct ttcttcgcaa tataaagaag gcggagtaat aagctttaac 2280 ttcaattcaa tagatatggc taaaggcgat ttaaaagtac aaatggcctg tggtgaaggt 2340 tgcacgcgtg aagtagatat cacaactatc gcacgcgact tggaaggcaa aggctggcag 2400 tcgttaacag tgcccttagc gtgctttgca cacgaaggcg acgatttcac ccatattact 2460 gcgccgttta acttatttgc cggtggaaaa ggtcaagttg ctgtagccaa cattcgcata 2520 ctgcgcgccg gtacacaaac cgtgccgtgt gtattgccta aagatgtttc cgtaacgcca 2580 gagccgctga atgctagctg ggcgatagat tggtggatgc cgcgccacaa agaaaaactg 2640 gcgcgtatcc agcaaggtaa tgtggattta ctaatgattg gcgattccat tacccacggc 2700 tgggaagatg caggtaaaga cgtgtgggcg caatattacg cgcaccgcaa tgcagtggac 2760 ttaggcttta gtggcgaccg aaccgaaaac gtattgtggc gcttacagca cggcgaagca 2820 gacggtatta agcctaaagt ggcagtggtt atgattggta ccaacaatgc cggccatcgt 2880 cacgagcctt cgcactacac agccaagggt gttgcggctg tcgttgctga attgcaaaaa 2940 cgattgcctg aaacaaagat attattactg ggtatattcc ctcgcggcga aaccagtgaa 3000 gaccctttgc gggtattaaa tgccaaaacc aatactcttt tggcgaaaat ggccgacgga 3060 gagaaggtgg tgtatttgaa tatcaataaa acgtttttag atgaaaacgg cgtattgcct 3120 aaagatataa tgcccgacct attgcacccc aatgaaaagg ggtacgcatt gtgggcgaaa 3180 gcgatggaac ccacccttaa aaaaatgctg ggcgaatag 3219 <210> 35 <211> 862 <212> PRT <213> Microbulbifer degradans <400> 35 Met Leu Lys Lys Ile Asn Lys Lys Gly Leu Ala Leu Ser Leu Ala Ile 1 5 10 15 Ala Ala Met Leu Ser Gly Cys Asn Glu Gly Asp Ser Asn Lys Thr Lys             20 25 30 Pro Ser Ala Glu Thr Leu Ser Ala Thr Gln Ala Ser Asn Thr Val Ala         35 40 45 Asn Pro Ser Ile Trp Pro Lys Val Thr Ser Lys Val Ala Lys Asp Ala     50 55 60 Lys Met Glu Ala Asp Ile Ser Ala Ile Leu Ser Gly Met Thr Leu Glu 65 70 75 80 Gln Lys Val Ala Gln Met Ile Gln Pro Glu Ile Arg Ala Phe Ser Lys                 85 90 95 Glu Asp Met Lys Lys Tyr Gly Phe Gly Ser Tyr Leu Asn Gly Gly Gly             100 105 110 Ala Phe Pro Asn Asp Asn Lys His Ser Thr Met Ala Asp Trp Val Ala         115 120 125 Leu Ala Asp Asp Met Tyr Glu Ala Ser Ile Asp Asp Ser Ile Asp Gly     130 135 140 Ser Thr Ile Pro Thr Met Trp Gly Thr Asp Ala Val His Gly His Asn 145 150 155 160 Asn Val Val Lys Ala Thr Ile Phe Pro His Asn Ile Gly Leu Gly Ala                 165 170 175 Met His Asn Pro Lys Leu Met Gln Gln Ile Gly Ala Ala Thr Ala Lys             180 185 190 Val Val Gln Val Thr Gly Ile Asp Trp Val Phe Ala Pro Thr Val Ala         195 200 205 Val Val Arg Asp Asp Arg Trp Gly Arg Thr Tyr Glu Gly Tyr Ser Glu     210 215 220 Asp Pro Ala Ile Val Lys Glu Tyr Ala Arg Ala Met Val Ile Gly Met 225 230 235 240 Gln Gly Glu Ala Asn Ser Glu Ala Phe Met Gly Asp Gly Thr Val Ile                 245 250 255 Ala Thr Ala Lys His Phe Leu Gly Asp Gly Gly Thr Asp Lys Gly Asp             260 265 270 Asp Gln Gly Asn Asn Leu Ser Thr Glu Gln Glu Leu Ile Asp Ile His         275 280 285 Ala Gln Gly Tyr Ile Ser Ala Ile Glu Glu Gly Val Gln Thr Ile Met     290 295 300 Ala Ser Phe Asn Ser Trp Asn Gly Glu Lys Met His Gly Asn Lys Ser 305 310 315 320 Leu Leu Thr Asp Val Leu Lys Lys Gln Met Gly Phe Asp Gly Leu Val                 325 330 335 Val Gly Asp Trp Asp Gly His Gly Gln Val Lys Gly Cys Ser Asn Ala             340 345 350 Ser Cys Ala Gln Ala Ile Asn Ala Gly Val Asp Ile Ile Met Val Pro         355 360 365 Asn Glu Trp Lys Pro Met Phe Glu Asn Thr Val Ala Gln Val Lys Ser     370 375 380 Gly Glu Ile Ser Glu Ala Arg Ile Asn Asp Ala Val Thr Arg Ile Leu 385 390 395 400 Arg Val Lys Met Arg Ala Gly Ile Phe Asp Gly Val Lys Pro Ser Asp                 405 410 415 Arg Ala Phe Ala Ala Glu Glu Lys Tyr Leu Gly Ser Ala Glu Asn Arg             420 425 430 Ala Ile Ala Arg Gln Ala Val Arg Glu Ser Leu Val Leu Leu Lys Asn         435 440 445 Gln Asn Lys Leu Leu Pro Leu Asp Arg Lys Met Asn Val Leu Met Ala     450 455 460 Gly Ser Gly Ala Asp Asn Ile Gly Lys Gln Ser Gly Gly Trp Thr Leu 465 470 475 480 Ser Trp Gln Gly Thr Gly Asn Val Asn Ser Asp Phe Pro Gly Ala Thr                 485 490 495 Ser Ile Tyr Asp Gly Val Asn Gln Val Val Ser Ser Ala Gly Gly Lys             500 505 510 Val Glu Leu Ser Glu Asn Gly Asn Tyr Gln Ala Lys Pro Asp Val Ala         515 520 525 Ile Val Val Phe Gly Glu Asn Pro Tyr Ala Glu Gly Val Gly Asp Ile     530 535 540 Glu Gly Ile Glu Tyr Gln Leu Asn Asn Lys Arg Asp Ile Asn Leu Leu 545 550 555 560 Gln Lys Leu Lys Ala Asp Gly Ile Pro Val Val Ser Val Phe Leu Thr                 565 570 575 Gly Arg Pro Leu Trp Val Asn Lys Glu Leu Asn Ala Ser Asp Ala Phe             580 585 590 Val Ala Ala Trp Leu Pro Gly Ser Glu Gly Val Gly Val Ser Asp Val         595 600 605 Leu Phe Lys Lys Ala Asp Gly Ser Ile Asn Tyr Asp Phe Lys Gly Lys     610 615 620 Leu Thr Tyr Ser Trp Pro Lys Tyr Asp Asp Gln Val Val Ile Asn Lys 625 630 635 640 Gly Asp Lys Asp Tyr Ala Pro Leu Tyr Pro Tyr Gly Tyr Gly Leu Thr                 645 650 655 Tyr Ser Asp Val Asp Thr Gln Gly Asp Asp Leu Pro Glu Glu Thr Lys             660 665 670 Val Lys Ile Gly Arg Ala Asp Asp Glu Pro Met Ala Ile Phe Asp Ser         675 680 685 Leu Pro Gln Ser Asp Leu Gly Phe Phe Leu Gly Asp Lys Ala Asn Trp     690 695 700 Val Val Pro Ile Ala Thr Ser Val Val Thr Thr His Asn Ser Asp Asn 705 710 715 720 Leu Thr Met Arg Thr Tyr Asn Trp Lys Val Gln Glu Asp Ala Arg Gln                 725 730 735 Leu Ile Trp Lys Gly Asp Ser Lys Ala Asn Ala Phe Phe Ala Trp Pro             740 745 750 Asp Pro His Asn Met Gln Gly Met Leu Glu His Lys Ala Ala Tyr Ser         755 760 765 Phe Ser Ile Lys Val Asp Lys Ala Pro Ala Gly Asp Leu Thr Leu Gly     770 775 780 Ile His Cys Met Glu Glu Cys Gly Lys Lys Leu Val Leu Asn Glu Ala 785 790 795 800 Leu Ser Lys Ile Pro Ala Gly Glu Trp Gly Glu Leu Thr Ile Asp Leu                 805 810 815 Ala Cys Ile Ala Asp Ala Glu Ala Leu Ala Glu Val Arg Ser Pro Phe             820 825 830 Met Leu Ser Thr Asp Ala Pro Ala Ser Ile Val Phe Gly Asp Val Lys         835 840 845 Leu Val Pro Gly Gly Ala Asp Ser Ala Ala Ile Lys Cys Asp     850 855 860 <210> 36 <211> 2540 <212> DNA <213> Microbulbifer degradans <400> 36 atgctcaaaa agataaacaa gaaaggtctt gctttaagct tagcaattgc agcaatgcta 60 agcggctgca acgaaggcga cagcaacaaa accaaaccaa gtgcggaaac cctctccgct 120 actcaagcca gtaacactgt agccaacccc agcatttggc ccaaggtaac tagcaaggtt 180 gccaaagacg ccaaaatgga agcagatata agcgcaatac tcagcggtat gacccttgag 240 caaaaagtag cccaaatgat ccaacccgaa attcgtgcct tcagcaaaga agacatgaaa 300 aagtatggtt ttggctccta ccttaacggt ggcggcgcat tccctaacga caacaaacat 360 tccaccatgg ccgactgggt tgccctagcc gacgacatgt atgaagcctc tatagacgac 420 agcatagacg gcagcactat tccaaccatg tggggtaccg atgcagtaca cggccacaac 480 aacgtggtta aagcgactat tttcccacac aacattggcc ttggcgccat gcataacccc 540 aagctcatgc agcaaatagg cgctgccacg gctaaagtgg tacaagttac tggtatcgac 600 tgggtatttg cgcccactgt tgcggtagtg cgcgacgacc gctggggccg tacttacgag 660 ggctactctg aagaccccgc catagtaaaa gaatacgctc gcgccatggt tattggcatg 720 cagggcgaag ccaatagcga agcgtttatg ggtgacggca ctgttatagc caccgccaaa 780 cactttttgg gcgatggcgg caccgacaaa ggcgacgacc aaggcaacaa cttatccacc 840 gaacaagaat taattgatat tcacgcccaa ggctatataa gcgccattga agaaggtgtg 900 caaactatca tggcatcttt caatagctgg aatggcgaaa agatgcacgg caataaatct 960 ctgcttaccg atgtccttaa aaagcaaatg ggctttgacg gtttggtggt tggcgattgg 1020 gatggccacg gccaagtaaa aggttgctct aatgcaagct gtgcccaagc catcaacgcc 1080 ggtgtcgata tcatcatggt acccaatgag tggaaaccca tgttcgaaaa caccgttgca 1140 caagttaaaa gcggcgaaat ctctgaagcg cgaattaacg atgcagttac ccgtatttta 1200 cgtgtaaaaa tgcgcgctgg tattttcgac ggtgttaaac catcggatcg cgccttcgca 1260 gcagaagaaa aatacctagg ctctgccgaa aaccgcgcta tcgctcgtca agctgtacgc 1320 gaatcgttag tgttgcttaa aaaccaaaac aaactgctgc cattagaccg caaaatgaac 1380 gttttaatgg cgggttctgg cgcagacaac atcggcaagc aaagtggtgg ttggacatta 1440 agctggcagg gtactggcaa cgtgaacagc gacttccctg gcgcaacatc tatttacgac 1500 ggcgttaacc aagtagtgag cagcgctggc ggtaaagtag agctaagcga aaacggcaac 1560 taccaagcca aaccagatgt agcgattgta gtatttggtg aaaaccctta cgcagaaggc 1620 gtaggcgata ttgaaggtat tgaataccaa ctaaacaata agcgcgatat caatttgtta 1680 caaaaactca aagccgatgg cattcctgtt gtatcggtat tcttaaccgg tcgtccactt 1740 tgggtaaaca aagagcttaa tgcctccgat gcttttgttg cagcttggct gccaggctct 1800 gaaggtgtag gcgtttctga tgtgctattc aaaaaagccg acggtagtat taactacgac 1860 tttaaaggca agctaactta ctcttggcca aagtatgatg accaagtagt aataaacaaa 1920 ggcgacaaag attacgcccc gctttaccct tatggttacg gcttaaccta cagcgatgtt 1980 gacacccaag gtgacgactt acctgaagaa accaaagtta aaattggccg cgctgacgac 2040 gagccaatgg ccatcttcga cagcctaccc caaagcgacc tcggcttctt ccttggcgac 2100 aaagccaact gggtagtacc tattgcaaca agtgtagtta caacgcacaa cagcgataac 2160 ctaaccatgc gcacctacaa ctggaaagta caagaagatg ctcgccagtt aatttggaaa 2220 ggcgacagca aagccaatgc cttctttgca tggccagacc cacacaatat gcaaggcatg 2280 ttagaacaca aagcggctta cagctttagc attaaagtag ataaagcacc cgctggcgac 2340 ctaacactag gcatacactg catggaagaa tgcggtaaaa aacttgtgct taacgaagcg 2400 cttagcaaaa ttcctgctgg tgagtgggga gagctaacaa tagatctagc ttgcatagca 2460 gatgccgaag ccttggccga agttcgctca cccttcatgc taagcaccga tgcacccgca 2520 tctatcgtgt ttggcgatgt 2540 <210> 37 <211> 862 <212> PRT <213> Microbulbifer degradans <400> 37 Met Leu Lys Lys Ile Asn Lys Lys Gly Leu Ala Leu Ser Leu Ala Ile 1 5 10 15 Ala Ala Met Leu Ser Gly Cys Asn Glu Gly Asp Ser Asn Lys Thr Lys             20 25 30 Pro Ser Ala Glu Thr Leu Ser Ala Thr Gln Ala Ser Asn Thr Val Ala         35 40 45 Asn Pro Ser Ile Trp Pro Lys Val Thr Ser Lys Val Ala Lys Asp Ala     50 55 60 Lys Met Glu Ala Asp Ile Ser Ala Ile Leu Ser Gly Met Thr Leu Glu 65 70 75 80 Gln Lys Val Ala Gln Met Ile Gln Pro Glu Ile Arg Ala Phe Ser Lys                 85 90 95 Glu Asp Met Lys Lys Tyr Gly Phe Gly Ser Tyr Leu Asn Gly Gly Gly             100 105 110 Ala Phe Pro Asn Asp Asn Lys His Ser Thr Met Ala Asp Trp Val Ala         115 120 125 Leu Ala Asp Asp Met Tyr Glu Ala Ser Ile Asp Asp Ser Ile Asp Gly     130 135 140 Ser Thr Ile Pro Thr Met Trp Gly Thr Asp Ala Val His Gly His Asn 145 150 155 160 Asn Val Val Lys Ala Thr Ile Phe Pro His Asn Ile Gly Leu Gly Ala                 165 170 175 Met His Asn Pro Lys Leu Met Gln Gln Ile Gly Ala Ala Thr Ala Lys             180 185 190 Val Val Gln Val Thr Gly Ile Asp Trp Val Phe Ala Pro Thr Val Ala         195 200 205 Val Val Arg Asp Asp Arg Trp Gly Arg Thr Tyr Glu Gly Tyr Ser Glu     210 215 220 Asp Pro Ala Ile Val Lys Glu Tyr Ala Arg Ala Met Val Ile Gly Met 225 230 235 240 Gln Gly Glu Ala Asn Ser Glu Ala Phe Met Gly Asp Gly Thr Val Ile                 245 250 255 Ala Thr Ala Lys His Phe Leu Gly Asp Gly Gly Thr Asp Lys Gly Asp             260 265 270 Asp Gln Gly Asn Asn Leu Ser Thr Glu Gln Glu Leu Ile Asp Ile His         275 280 285 Ala Gln Gly Tyr Ile Ser Ala Ile Glu Glu Gly Val Gln Thr Ile Met     290 295 300 Ala Ser Phe Asn Ser Trp Asn Gly Glu Lys Met His Gly Asn Lys Ser 305 310 315 320 Leu Leu Thr Asp Val Leu Lys Lys Gln Met Gly Phe Asp Gly Leu Val                 325 330 335 Val Gly Asp Trp Asp Gly His Gly Gln Val Lys Gly Cys Ser Asn Ala             340 345 350 Ser Cys Ala Gln Ala Ile Asn Ala Gly Val Asp Ile Ile Met Val Pro         355 360 365 Asn Glu Trp Lys Pro Met Phe Glu Asn Thr Val Ala Gln Val Lys Ser     370 375 380 Gly Glu Ile Ser Glu Ala Arg Ile Asn Asp Ala Val Thr Arg Ile Leu 385 390 395 400 Arg Val Lys Met Arg Ala Gly Ile Phe Asp Gly Val Lys Pro Ser Asp                 405 410 415 Arg Ala Phe Ala Ala Glu Glu Lys Tyr Leu Gly Ser Ala Glu Asn Arg             420 425 430 Ala Ile Ala Arg Gln Ala Val Arg Glu Ser Leu Val Leu Leu Lys Asn         435 440 445 Gln Asn Lys Leu Leu Pro Leu Asp Arg Lys Met Asn Val Leu Met Ala     450 455 460 Gly Ser Gly Ala Asp Asn Ile Gly Lys Gln Ser Gly Gly Trp Thr Leu 465 470 475 480 Ser Trp Gln Gly Thr Gly Asn Val Asn Ser Asp Phe Pro Gly Ala Thr                 485 490 495 Ser Ile Tyr Asp Gly Val Asn Gln Val Val Ser Ser Ala Gly Gly Lys             500 505 510 Val Glu Leu Ser Glu Asn Gly Asn Tyr Gln Ala Lys Pro Asp Val Ala         515 520 525 Ile Val Val Phe Gly Glu Asn Pro Tyr Ala Glu Gly Val Gly Asp Ile     530 535 540 Glu Gly Ile Glu Tyr Gln Leu Asn Asn Lys Arg Asp Ile Asn Leu Leu 545 550 555 560 Gln Lys Leu Lys Ala Asp Gly Ile Pro Val Val Ser Val Phe Leu Thr                 565 570 575 Gly Arg Pro Leu Trp Val Asn Lys Glu Leu Asn Ala Ser Asp Ala Phe             580 585 590 Val Ala Ala Trp Leu Pro Gly Ser Glu Gly Val Gly Val Ser Asp Val         595 600 605 Leu Phe Lys Lys Ala Asp Gly Ser Ile Asn Tyr Asp Phe Lys Gly Lys     610 615 620 Leu Thr Tyr Ser Trp Pro Lys Tyr Asp Asp Gln Val Val Ile Asn Lys 625 630 635 640 Gly Asp Lys Asp Tyr Ala Pro Leu Tyr Pro Tyr Gly Tyr Gly Leu Thr                 645 650 655 Tyr Ser Asp Val Asp Thr Gln Gly Asp Asp Leu Pro Glu Glu Thr Lys             660 665 670 Val Lys Ile Gly Arg Ala Asp Asp Glu Pro Met Ala Ile Phe Asp Ser         675 680 685 Leu Pro Gln Ser Asp Leu Gly Phe Phe Leu Gly Asp Lys Ala Asn Trp     690 695 700 Val Val Pro Ile Ala Thr Ser Val Val Thr Thr His Asn Ser Asp Asn 705 710 715 720 Leu Thr Met Arg Thr Tyr Asn Trp Lys Val Gln Glu Asp Ala Arg Gln                 725 730 735 Leu Ile Trp Lys Gly Asp Ser Lys Ala Asn Ala Phe Phe Ala Trp Pro             740 745 750 Asp Pro His Asn Met Gln Gly Met Leu Glu His Lys Ala Ala Tyr Ser         755 760 765 Phe Ser Ile Lys Val Asp Lys Ala Pro Ala Gly Asp Leu Thr Leu Gly     770 775 780 Ile His Cys Met Glu Glu Cys Gly Lys Lys Leu Val Leu Asn Glu Ala 785 790 795 800 Leu Ser Lys Ile Pro Ala Gly Glu Trp Gly Glu Leu Thr Ile Asp Leu                 805 810 815 Ala Cys Ile Ala Asp Ala Glu Ala Leu Ala Glu Val Arg Ser Pro Phe             820 825 830 Met Leu Ser Thr Asp Ala Pro Ala Ser Ile Val Phe Gly Asp Val Lys         835 840 845 Leu Val Pro Gly Gly Ala Asp Ser Ala Ala Ile Lys Cys Asp     850 855 860 <210> 38 <211> 2589 <212> DNA <213> Microbulbifer degradans <400> 38 atgctcaaaa agataaacaa gaaaggtctt gctttaagct tagcaattgc agcaatgcta 60 agcggctgca acgaaggcga cagcaacaaa accaaaccaa gtgcggaaac cctctccgct 120 actcaagcca gtaacactgt agccaacccc agcatttggc ccaaggtaac tagcaaggtt 180 gccaaagacg ccaaaatgga agcagatata agcgcaatac tcagcggtat gacccttgag 240 caaaaagtag cccaaatgat ccaacccgaa attcgtgcct tcagcaaaga agacatgaaa 300 aagtatggtt ttggctccta ccttaacggt ggcggcgcat tccctaacga caacaaacat 360 tccaccatgg ccgactgggt tgccctagcc gacgacatgt atgaagcctc tatagacgac 420 agcatagacg gcagcactat tccaaccatg tggggtaccg atgcagtaca cggccacaac 480 aacgtggtta aagcgactat tttcccacac aacattggcc ttggcgccat gcataacccc 540 aagctcatgc agcaaatagg cgctgccacg gctaaagtgg tacaagttac tggtatcgac 600 tgggtatttg cgcccactgt tgcggtagtg cgcgacgacc gctggggccg tacttacgag 660 ggctactctg aagaccccgc catagtaaaa gaatacgctc gcgccatggt tattggcatg 720 cagggcgaag ccaatagcga agcgtttatg ggtgacggca ctgttatagc caccgccaaa 780 cactttttgg gcgatggcgg caccgacaaa ggcgacgacc aaggcaacaa cttatccacc 840 gaacaagaat taattgatat tcacgcccaa ggctatataa gcgccattga agaaggtgtg 900 caaactatca tggcatcttt caatagctgg aatggcgaaa agatgcacgg caataaatct 960 ctgcttaccg atgtccttaa aaagcaaatg ggctttgacg gtttggtggt tggcgattgg 1020 gatggccacg gccaagtaaa aggttgctct aatgcaagct gtgcccaagc catcaacgcc 1080 ggtgtcgata tcatcatggt acccaatgag tggaaaccca tgttcgaaaa caccgttgca 1140 caagttaaaa gcggcgaaat ctctgaagcg cgaattaacg atgcagttac ccgtatttta 1200 cgtgtaaaaa tgcgcgctgg tattttcgac ggtgttaaac catcggatcg cgccttcgca 1260 gcagaagaaa aatacctagg ctctgccgaa aaccgcgcta tcgctcgtca agctgtacgc 1320 gaatcgttag tgttgcttaa aaaccaaaac aaactgctgc cattagaccg caaaatgaac 1380 gttttaatgg cgggttctgg cgcagacaac atcggcaagc aaagtggtgg ttggacatta 1440 agctggcagg gtactggcaa cgtgaacagc gacttccctg gcgcaacatc tatttacgac 1500 ggcgttaacc aagtagtgag cagcgctggc ggtaaagtag agctaagcga aaacggcaac 1560 taccaagcca aaccagatgt agcgattgta gtatttggtg aaaaccctta cgcagaaggc 1620 gtaggcgata ttgaaggtat tgaataccaa ctaaacaata agcgcgatat caatttgtta 1680 caaaaactca aagccgatgg cattcctgtt gtatcggtat tcttaaccgg tcgtccactt 1740 tgggtaaaca aagagcttaa tgcctccgat gcttttgttg cagcttggct gccaggctct 1800 gaaggtgtag gcgtttctga tgtgctattc aaaaaagccg acggtagtat taactacgac 1860 tttaaaggca agctaactta ctcttggcca aagtatgatg accaagtagt aataaacaaa 1920 ggcgacaaag attacgcccc gctttaccct tatggttacg gcttaaccta cagcgatgtt 1980 gacacccaag gtgacgactt acctgaagaa accaaagtta aaattggccg cgctgacgac 2040 gagccaatgg ccatcttcga cagcctaccc caaagcgacc tcggcttctt ccttggcgac 2100 aaagccaact gggtagtacc tattgcaaca agtgtagtta caacgcacaa cagcgataac 2160 ctaaccatgc gcacctacaa ctggaaagta caagaagatg ctcgccagtt aatttggaaa 2220 ggcgacagca aagccaatgc cttctttgca tggccagacc cacacaatat gcaaggcatg 2280 ttagaacaca aagcggctta cagctttagc attaaagtag ataaagcacc cgctggcgac 2340 ctaacactag gcatacactg catggaagaa tgcggtaaaa aacttgtgct taacgaagcg 2400 cttagcaaaa ttcctgctgg tgagtgggga gagctaacaa tagatctagc ttgcatagca 2460 gatgccgaag ccttggccga agttcgctca cccttcatgc taagcaccga tgcacccgca 2520 tctatcgtgt ttggcgatgt gaagttagta cctggcggtg cagatagcgc agctattaag 2580 tgtgactaa 2589 <210> 39 <211> 461 <212> PRT <213> Microbulbifer degradans <400> 39 Met Lys Thr Phe Asn Pro Asp Phe Val Trp Gly Ala Ala Ser Ser Ala 1 5 10 15 Tyr Gln Val Glu Gly Ala Thr Thr Thr Thr Asp Gly Arg Gly Pro Ser Ile             20 25 30 Trp Asp Ala Phe Ser Ser Ile Pro Gly Lys Thr Tyr His Asn Gln Asn         35 40 45 Ala Asp Ile Ala Cys Asp His Tyr Asn Arg Trp Gln Glu Asp Val Ala     50 55 60 Ile Met Lys Glu Met Gly Leu Lys Ala Tyr Arg Phe Ser Ile Ser Trp 65 70 75 80 Ser Arg Ile Phe Pro Thr Gly Arg Gly Glu Val Asn Glu Lys Gly Val                 85 90 95 Ala Phe Tyr Asn Asn Leu Ile Asp Glu Leu Ile Lys Asn Asp Ile Thr             100 105 110 Pro Trp Val Thr Leu Phe His Trp Asp Phe Pro Leu Ala Leu Gln Met         115 120 125 Glu Met Asp Gly Leu Leu Asn Pro Ala Ile Ala Asp Glu Phe Ala Asn     130 135 140 Tyr Ala Lys Leu Cys Phe Ala Arg Phe Gly Asp Arg Val Thr His Trp 145 150 155 160 Ile Thr Leu Asn Glu Pro Trp Cys Ser Ala Met Leu Gly His Gly Met                 165 170 175 Gly Ser Lys Ala Pro Gly Arg Val Ser Lys Asp Glu Pro Tyr Ile Ala             180 185 190 Ala His Asn Leu Leu Arg Ala His Gly Lys Met Val Asp Ile Tyr Arg         195 200 205 Arg Glu Phe Gln Pro Thr Gln Lys Gly Met Ile Gly Ile Ala Asn Asn     210 215 220 Cys Asp Trp Arg Glu Pro Lys Thr Asp Ser Glu Leu Asp Lys Lys Ala 225 230 235 240 Ala Glu Arg Ala Leu Glu Phe Phe Val Ser Trp Phe Ala Asp Pro Ile                 245 250 255 Tyr Leu Gly Asp Tyr Pro Ala Ser Met Arg Glu Arg Leu Gly Glu Arg             260 265 270 Leu Pro Thr Phe Ser Asp Glu Asp Ile Ala Leu Ile Lys Asn Ser Ser         275 280 285 Asp Phe Phe Gly Leu Asn His Tyr Thr Thr Met Leu Ala Glu Gln Thr     290 295 300 His Glu Gly Asp Val Val Glu Asp Thr Ile Arg Gly Asn Gly Gly Ile 305 310 315 320 Ser Glu Asp Gln Met Val Thr Leu Ser Lys Asp Pro Ser Trp Glu Gln                 325 330 335 Thr Asp Met Glu Trp Ser Ile Val Pro Trp Gly Cys Lys Lys Leu Leu             340 345 350 Ile Trp Leu Ser Glu Arg Tyr Asn Tyr Pro Asp Ile Tyr Ile Thr Glu         355 360 365 Asn Gly Cys Ala Leu Pro Asp Glu Asp Asp Val Asn Ile Ala Ile Asn     370 375 380 Asp Thr Arg Arg Val Asp Phe Tyr Arg Gly Tyr Ile Asp Ala Cys His 385 390 395 400 Gln Ala Ile Glu Ala Gly Val Lys Leu Lys Gly Tyr Phe Ala Trp Thr                 405 410 415 Leu Met Asp Asn Tyr Glu Trp Glu Glu Gly Tyr Thr Lys Arg Phe Gly             420 425 430 Leu Asn His Val Asp Phe Thr Thr Gly Lys Arg Thr Pro Lys Gln Ser         435 440 445 Ala Ile Trp Tyr Ser Thr Leu Ile Lys Asp Gly Gly Phe     450 455 460 <210> 40 <211> 1386 <212> DNA <213> Microbulbifer degradans <400> 40 atgaaaacct ttaacccaga tttcgtatgg ggagcagcca gttccgccta tcaggtagaa 60 ggcgccacca ccaccgatgg cagaggcccc agtatttggg atgcgttcag ttccattccc 120 ggtaaaacct accacaacca aaacgccgac atagcctgcg accactacaa ccgctggcaa 180 gaagacgtgg ccataatgaa agagatgggg ctaaaggctt accgcttttc tatttcttgg 240 tcgcgcatat tccctactgg gcgcggcgaa gttaacgaaa aaggcgtagc cttttacaac 300 aaccttatcg acgaattaat aaaaaacgac attacccctt gggtaaccct atttcactgg 360 gactttcctc tggcactgca aatggaaatg gacggcctac ttaaccccgc catcgccgac 420 gaattcgcca actacgccaa gctgtgtttc gcgcgctttg gcgaccgcgt tacccactgg 480 attaccctaa acgaaccttg gtgcagtgcc atgcttggcc acggcatggg cagcaaagcc 540 cctggccgcg tatctaagga tgaaccctat atagccgccc acaacttgct gcgtgcacac 600 ggcaaaatgg tagatattta ccggcgcgaa tttcagccca cacaaaaagg catgataggc 660 atagccaaca attgcgactg gcgcgaaccc aaaaccgatt ctgaattaga taaaaaagca 720 gccgagcgcg ccctagaatt ttttgtaagc tggtttgccg accccattta tttgggcgac 780 tacccagcca gcatgcgcga gcgcttgggt gagcgtttac ccacctttag cgacgaagac 840 attgcgctaa taaaaaactc tagcgacttt tttggtttga atcactacac caccatgctt 900 gccgaacaaa cccacgaagg tgacgttgtt gaagatacta ttcgcggcaa cggcggcata 960 tcggaagacc aaatggtcac cctctccaaa gacccaagct gggaacaaac cgacatggag 1020 tggagcattg tgccctgggg ctgtaaaaaa ttattaatct ggttaagcga gcgctacaac 1080 taccccgaca tttacattac cgaaaacggc tgcgccctac ccgacgaaga cgacgtaaac 1140 atagccatta acgatacacg ccgcgtagat ttttaccgcg gttatatcga tgcgtgtcac 1200 caagcaatag aggccggcgt aaaactaaaa ggctattttg catggacact tatggataac 1260 tacgaatggg aagaaggcta caccaaacgc tttggcttaa accatgtaga tttcaccaca 1320 ggcaaacgca cacctaaaca gtctgcaatt tggtatagca cgttaattaa agatggtggg 1380 ttctag 1386 <210> 41 <211> 444 <212> PRT <213> Microbulbifer degradans <400> 41 Met Asn Arg Leu Thr Leu Pro Pro Ser Ser Arg Leu Arg Ser Lys Glu 1 5 10 15 Phe Thr Phe Gly Val Ala Thr Ser Ser Tyr Gln Ile Glu Gly Gly Ile             20 25 30 Asp Ser Arg Leu Pro Cys Asn Trp Asp Thr Phe Cys Glu Gln Pro Asn         35 40 45 Thr Ile Ile Asp Asn Thr Asn Gly Ala Ile Ala Cys Asp His Ile Asn     50 55 60 Arg Trp Gln Asp Asp Ile Glu Leu Ile Ala Asn Leu Gly Val Asp Ala 65 70 75 80 Tyr Arg Phe Ser Ile Ala Trp Gly Arg Val Ile Asn Leu Asp Gly Ser                 85 90 95 Leu Asn Asn Glu Gly Val Thr Phe Tyr Lys Asn Ile Leu Thr Lys Leu             100 105 110 Arg Glu Lys Asn Leu Lys Ala Tyr Ile Thr Leu Tyr His Trp Asp Leu         115 120 125 Pro Gln His Leu Glu Asp Ala Gly Gly Trp Leu Asn Arg Asp Thr Ala     130 135 140 Tyr Lys Phe Arg Asp Tyr Val Asn Leu Ile Thr Gln Ala Leu Asp Asp 145 150 155 160 Asp Val Phe Cys Tyr Thr Thr Leu Asn Glu Pro Phe Cys Ser Ala Tyr                 165 170 175 Leu Gly Tyr Glu Ile Gly Val His Ala Pro Gly Ile Lys Asp Leu Ala             180 185 190 Ser Gly Arg Lys Ala Ala His His Leu Leu Leu Ala His Gly Leu Ala         195 200 205 Met Gln Val Leu Arg Lys Asn Cys Pro Asn Ser Leu Ser Gly Ile Val     210 215 220 Leu Asn Met Ser Pro Cys Tyr Ala Gly Ser Asn Ala Gln Ala Asp Ile 225 230 235 240 Asp Ala Ala Lys Arg Ala Asp Asp Leu Leu Phe Gln Trp Tyr Ala Gln                 245 250 255 Pro Leu Leu Thr Gly Cys Tyr Pro Asp Ala Ile Asn Ser Leu Pro Asp             260 265 270 Asn Ala Lys Pro Pro Ile Cys Glu Gly Asp Met Ala Leu Ile Ser Gln         275 280 285 Pro Leu Asp Tyr Leu Gly Leu Asn Tyr Tyr Thr Arg Ala Val Phe Phe     290 295 300 Ala Asp Gly Asn Gly Gly Phe Thr Glu Gln Val Pro Glu Gly Val Glu 305 310 315 320 Leu Thr Asp Met Gly Trp Glu Val Tyr Pro Gln Gly Leu Thr Asp Leu                 325 330 335 Leu Ile Asp Leu Asn Gln Arg Tyr Thr Leu Pro Pro Leu Leu Ile Thr             340 345 350 Glu Asn Gly Ala Ala Met Val Asp Glu Leu Val Asn Gly Glu Val Asn         355 360 365 Asp Ile Ala Arg Ile Asn Tyr Phe Gln Thr His Leu Gln Ala Val His     370 375 380 Asn Ala Ile Glu Gln Gly Val Asp Val Arg Gly Tyr Phe Ala Trp Ser 385 390 395 400 Leu Met Asp Asn Phe Glu Trp Ala Leu Gly Tyr Ser Lys Arg Phe Gly                 405 410 415 Ile Thr Tyr Val Asp Tyr Gln Thr Gln Lys Arg Thr Leu Lys Ala Ser             420 425 430 Gly His Ala Phe Ala Glu Phe Val Ser Ser Arg Ser         435 440 <210> 42 <211> 1335 <212> DNA <213> Microbulbifer degradans <400> 42 atgaatagac ttacactacc gccttcttct cgtttgcgca gcaaagagtt tacctttggt 60 gttgcaacgt cgtcttacca aattgaaggc ggcatagatt ctcgcctgcc ctgtaattgg 120 gatacgttct gtgagcagcc caataccatt attgataaca ccaacggcgc cattgcttgc 180 gaccacataa atagatggca agacgatata gaacttattg ccaacctagg ggtagatgcc 240 taccgctttt ctattgcgtg gggccgtgtt attaatttag acggcagcct caataatgaa 300 ggcgttacat tttacaaaaa tattttaact aagcttcgcg aaaagaattt aaaagcttat 360 ataacgctat accactggga cttgccacaa catttagaag atgctggcgg ctggcttaac 420 cgcgataccg cctacaagtt tcgcgactat gtaaacctta taacccaagc gcttgatgac 480 gatgtatttt gctacacaac gttaaacgag cccttttgca gtgcctacct tggctatgaa 540 attggtgtac acgcaccggg tataaaagac ttagccagtg ggcgcaaagc cgcacaccat 600 ttattacttg cccatggctt agctatgcaa gtgctgcgaa aaaactgccc caatagttta 660 agcggcatag tgttaaacat gagcccttgt tacgccggca gcaacgcaca agcagatata 720 gatgcagcaa aacgcgcgga cgatttatta tttcagtggt atgcacaacc gctacttact 780 ggctgctacc ctgatgcaat aaacagcctg ccagacaatg ccaaaccacc tatttgtgaa 840 ggcgacatgg cgttaataag ccaaccttta gattatttag gccttaacta ctatacccgc 900 gcagtatttt ttgccgacgg taatggcggt tttaccgaac aagtacctga gggtgtagag 960 ctaaccgata tgggctggga agtttacccg caaggcttaa ccgatttact aatagaccta 1020 aaccaacgct ataccctacc cccgttactt attaccgaaa acggcgcagc aatggtggac 1080 gaacttgtta acggcgaagt taacgatatt gcccgaataa attattttca aacccattta 1140 caagcggtac acaacgccat tgaacaaggt gttgatgtac gcggttattt tgcttggagc 1200 ctaatggata attttgagtg ggcactgggt tacagcaaac gattcggtat tacctatgta 1260 gattaccaaa cacaaaagcg aacgctaaaa gccagcggcc acgcatttgc tgagtttgtc 1320 tcgagtagga gctaa 1335 <210> 43 <211> 866 <212> PRT <213> Microbulbifer degradans <400> 43 Met Leu Leu Ser Leu Lys Asn Thr Gln Leu Lys Arg Ser Met Asn Met 1 5 10 15 Asn Leu Lys His Leu Phe Leu Val Ala Leu Ala Leu Asn Ile Ala Ala             20 25 30 Cys Asn Val Lys Glu Pro Ala Ala Thr Asn Asp Asn His Ile Ser Tyr         35 40 45 Gln Ala Ala Arg Glu Ala Arg Leu Ala Lys Val Glu Ala Glu Val Glu     50 55 60 Arg Leu Leu Pro Leu Leu Thr Leu Glu Glu Lys Ala Ser Leu Val His 65 70 75 80 Ala Asn Ser Lys Phe Ser Ile Ala Ser Ile Glu Arg Leu Gly Ile His                 85 90 95 Glu Met Trp Met Ser Asp Gly Pro His Gly Val Arg Tyr Gln Ile Glu             100 105 110 Arg His Gly Trp Ala Pro Ala Gly Trp Thr Asp Asp Asn Ser Thr Tyr         115 120 125 Leu Pro Pro Leu Thr Thr Val Ala Ala Ser Trp Asn Pro Glu Ile Ala     130 135 140 Ala Leu His Gly Asp Val Leu Gly Ala Glu Ala Arg His Arg Arg Lys 145 150 155 160 Asp Val Ile Leu Gly Pro Gly Val Asn Leu Ala Arg Leu Pro Leu Tyr                 165 170 175 Gly Arg Asn Phe Glu Tyr Met Gly Glu Asp Pro Phe Leu Ala Ser Arg             180 185 190 Leu Ala Val Ala Glu Ile Lys Ala Ile Gln Glu Asn Asp Val Ala Ala         195 200 205 Cys Ile Lys His Phe Ala Leu Asn Asn Gln Glu Leu Asn Arg Thr Gly     210 215 220 Val Asn Ala Lys Pro Asp Glu Arg Thr Leu Arg Glu Val Tyr Leu Pro 225 230 235 240 Ala Phe Glu Ala Ala Val Lys Glu Ala Gly Val His Thr Ile Met Gly                 245 250 255 Ala Tyr Asn Glu Phe Arg Gly Thr Asn Ala Asn Gln Ser Lys His Leu             260 265 270 Val Met Asp Ile Leu Lys Gly Glu Trp Gly Tyr Lys Gly Val Leu Leu         275 280 285 Thr Asp Trp Asn Val Asp Ile Asn Thr Tyr Asp Ala Ala Val Asn Gly     290 295 300 Leu Asp Ile Glu Met Gly Thr Asn Val Asp Ser Tyr Asp Asp Tyr Met 305 310 315 320 Leu Ala Gln Pro Met Ile Asp Met Ile Lys Ala Gly Ser Ile Pro Glu                 325 330 335 Ser Val Leu Asp Asp Lys Val Arg Arg Ile Leu Arg Val Gln Leu Ser             340 345 350 Ile Gly Met Met Asp Lys Tyr Arg Leu Ser Gly Glu Arg Asn Thr Ala         355 360 365 Lys His His Glu Ala Ala Arg Lys Ile Ala Ser Glu Gly Ile Val Leu     370 375 380 Leu Lys Asn Glu Asn Ile Leu Pro Leu Asn Lys Asn Lys Ile Lys Asn 385 390 395 400 Val Leu Val Leu Gly Pro Asn Ala Asp Lys Val His Gly Leu Gly Gly                 405 410 415 Gly Ser Ser Glu Val Pro Ala Leu Tyr Glu Ile Thr Pro Leu Gln Gly             420 425 430 Leu Lys Gln Lys Leu Gly Asp Asn Val Asn Ile Thr Val Met Arg Ala         435 440 445 Arg Tyr Asp Gly Val Leu Met Pro Ile Ala Ser Asp Tyr Val Thr Ser     450 455 460 Arg His Trp Thr Gly Thr Pro Ala Trp Asn Met Val Arg Tyr Ser Asp 465 470 475 480 Ala Ala Arg Thr Gln Ala Ile Gly Asp Ser Ala Ile Val Asp Ser Ala                 485 490 495 Tyr Ser Ser Pro Ala Gly Thr Thr Lys Glu Tyr Val Thr Met Thr Ala             500 505 510 Thr Ile Lys Pro Leu Lys Ser Gly Glu His Thr Leu Lys Thr Ser Val         515 520 525 Met Gly Asp Phe Glu Leu Lys Ile Asn Gly Lys Thr Thr Val Lys His     530 535 540 Ser Ser Thr Ser Gly Asp Val Val Thr Gln Lys Ile Ala Leu Asn Gly 545 550 555 560 Gly Glu Thr Tyr Ser Phe Glu Ile Leu Tyr Ser Gly Asn Lys Asn Phe                 565 570 575 Thr Leu Gly Trp Asp Ala Pro Gly Asp Leu Phe Thr Ala Glu Lys Glu             580 585 590 Tyr Ile Ala Ala Ala Lys Lys Ala Asp Val Val Phe Tyr Phe Gly Gly         595 600 605 Leu Thr His Gly Asp Asp Arg Glu Ala Ile Asp Arg Pro His Met Lys     610 615 620 Leu Pro Asn His Gln Asp Pro Val Ile Ser Lys Val Leu Ala Ala Asn 625 630 635 640 Pro Asn Thr Val Val Phe Leu Ile Ala Gly Ser Ala Val Glu Met Pro                 645 650 655 Trp Ala Asp Lys Ala Lys Ala Ile Val Trp Gly Trp Tyr Gly Gly Met             660 665 670 Glu Ala Gly Asn Ala Tyr Ala Asp Met Leu Phe Gly Asp Thr Asn Pro         675 680 685 Ser Gly Lys Met Pro Ile Thr Leu Pro Lys Ala Leu Glu Asp Thr Ala     690 695 700 Pro Ile Ala Leu Asn Asp Tyr Asn Pro Val Glu Ser Leu Tyr Thr Glu 705 710 715 720 Gly Val Phe Ile Gly Tyr Arg Trp Phe Glu Lys Gln Asn Ile Glu Pro                 725 730 735 Leu Phe Pro Phe Gly His Gly Leu Ser Tyr Thr Gln Phe Lys Tyr Asn             740 745 750 Asn Ile Lys Leu Ser Ser Ala Asn Ile Lys Gly Asp Gln Thr Val Thr         755 760 765 Val Ser Ala Thr Ile Thr Asn Thr Gly Lys Val Ala Gly Ala Glu Val     770 775 780 Val Gln Leu Tyr Leu His Asp Glu Gln Ala Ser Val Glu Arg Pro Ala 785 790 795 800 Lys Glu Leu Lys Gly Phe Gln Lys Val Phe Leu Lys Pro Gly Glu Ser                 805 810 815 Lys Ala Val Asn Ile Thr Leu Asn Lys Arg Ala Leu Ser Phe Trp Asp             820 825 830 Glu Asn Ser Asn Asp Trp Leu Ala Glu Thr Gly Lys Phe Asn Val Leu         835 840 845 Leu Gly Ala Ser Val Ser Asp Ile Arg Leu Gln Thr Ser Phe Gln Tyr     850 855 860 Gln Gln 865 <210> 44 <211> 2601 <212> DNA <213> Microbulbifer degradans <400> 44 atgctgctaa gcttaaaaaa cactcaactc aaaagaagta tgaacatgaa ccttaaacac 60 ctctttctgg ttgctttggc gctaaatatt gctgcgtgca atgtaaaaga gcccgcggcg 120 acaaatgata accacattag ctaccaagcc gctcgcgaag cgcgcttggc aaaagttgaa 180 gccgaagttg aacgcctgct gccactatta acactagaag aaaaagcctc tttggttcat 240 gcgaacagca aattctctat cgcctctatc gagcggctag gcattcacga aatgtggatg 300 tctgatggcc cccacggcgt gcgctatcaa atcgaacgcc acggctgggc accagcaggc 360 tggacagatg acaactccac ttacttacca ccgcttacta ccgtagccgc cagctggaac 420 cccgaaatag ctgcccttca cggcgatgta ctcggcgcag aagctcgcca ccgccgtaaa 480 gatgtaatat taggcccagg cgtaaactta gctcgcctgc cactttatgg tcgtaacttt 540 gaatatatgg gtgaagaccc cttcttggca tcacgtcttg ctgtggcaga aattaaagcc 600 attcaagaaa atgacgtggc cgcctgtatc aaacatttcg cgcttaacaa tcaagagctg 660 aatcgcaccg gcgtaaacgc caaacccgat gaacgcacat tacgcgaagt gtatttaccc 720 gccttcgaag ccgccgttaa agaagcgggc gtgcacacca taatgggggc ctacaatgaa 780 tttcgcggta ccaacgccaa ccaaagcaaa catttagtaa tggatattct aaaaggcgaa 840 tggggctaca aaggcgtgtt actcacagac tggaacgtag atatcaacac ttacgatgcc 900 gctgttaacg gcctcgatat cgaaatgggt acaaatgtag atagctacga cgactacatg 960 cttgcccaac caatgatcga catgattaaa gcgggcagca ttccagagtc agtacttgat 1020 gataaagttc gtcgcatact gcgcgtgcaa ctcagcatag gcatgatgga caaataccgc 1080 ttatctggtg agcgcaatac tgccaagcat cacgaagctg cacgcaaaat tgcatctgaa 1140 ggtattgtgc tactaaaaaa tgaaaacatt ctgccgctaa ataaaaacaa aattaaaaac 1200 gtattggtgc ttggccccaa cgcagacaaa gtgcacggtt taggcggtgg ctcgtcagaa 1260 gtgccagcac tttatgaaat aaccccgtta caagggttaa aacagaagct gggagataat 1320 gtaaacatta ccgttatgcg cgcacgctat gacggtgtgt taatgcctat cgccagtgat 1380 tatgttactt ctcgtcactg gaccggcaca cctgcatgga acatggtgcg ttactcggat 1440 gctgcgcgca cccaagctat tggcgactcc gccattgttg attcggctta ttcttcgcct 1500 gcaggcacga ctaaagaata cgtcaccatg accgccacaa ttaaaccgtt aaaatcgggc 1560 gagcacacac tcaaaacatc ggtgatgggc gatttcgaat taaaaattaa cggtaaaacc 1620 acagtaaaac atagcagcac tagcggcgat gtagtaaccc aaaaaatcgc cctcaacggc 1680 ggtgaaacat acagcttcga aattttatac agcggcaata aaaactttac cttgggctgg 1740 gatgcaccgg gagatttatt taccgcagaa aaagaataca tagccgccgc gaaaaaagcg 1800 gatgtagtgt tttactttgg cggcctaacc cacggcgacg accgcgaagc aattgaccgc 1860 cctcacatga agctgcctaa ccatcaagac ccagttatta gcaaagtatt agctgcaaac 1920 ccgaacacgg ttgtattttt aattgcaggc tctgctgtag aaatgccgtg ggccgataaa 1980 gctaaagcta ttgtgtgggg ctggtatggc ggtatggagg ccggtaacgc ctacgccgat 2040 atgctatttg gcgataccaa ccccagcggc aaaatgccaa taactttacc aaaggcactg 2100 gaagatactg ctccaatcgc actgaatgat tacaaccctg ttgaatcact ctacaccgag 2160 ggcgtgttta ttggttaccg ctggttcgaa aaacaaaaca tcgagccgct attcccgttc 2220 ggtcatggtt tgtcttatac ccagtttaag tacaacaata taaagctctc tagcgcgaac 2280 attaaaggcg accaaaccgt caccgtaagc gcaaccatta ccaatactgg caaagtggcc 2340 ggcgctgaag ttgtacaact gtatttgcat gacgagcaag caagcgtaga acgcccagca 2400 aaagaactta aaggtttcca aaaagtgttt ttaaagccgg gtgaaagcaa agcggtaaat 2460 attacgctta ataaacgcgc cctttcattt tgggatgaaa acagcaacga ctggcttgca 2520 gaaacaggta aatttaatgt gctattgggc gcatcagtaa gcgatatacg cttacaaact 2580 agcttccaat accagcagta a 2601 <210> 45 <211> 811 <212> PRT <213> Microbulbifer degradans <400> 45 Met Lys Phe Gly His Phe Asp Asp Lys Ala Arg Glu Tyr Val Ile Thr 1 5 10 15 Asp Pro Lys Thr Pro Tyr Pro Trp Ile Asn Tyr Leu Gly Asn Glu Asp             20 25 30 Phe Phe Ser Leu Val Ser Asn Thr Gly Gly Gly Tyr Ser Phe Tyr Lys         35 40 45 Asp Ala Lys Phe Arg Arg Leu Thr Arg Tyr Arg Tyr Asn Asn Val Pro     50 55 60 Val Asp Asn Gly Gly Lys Tyr Phe Tyr Ile Asn Asp Ser Gly Asp Val 65 70 75 80 Trp Ser Pro Gly Trp Lys Pro Val Lys Ala Glu Leu Asp Ala Tyr Ser                 85 90 95 Cys Ala His Gly Leu Ser Tyr Thr Arg Ile Thr Gly Glu Arg Asn Gly             100 105 110 Ile Gln Ala Glu Val Leu Ser Phe Ile Pro Leu Gly Thr Trp Ala Glu         115 120 125 Ile Gln Lys Val Ser Leu Lys Asn Thr Ser Gly Ala Thr Lys Lys Phe     130 135 140 Lys Leu Phe Ser Phe Ala Glu Trp Cys Leu Trp Asn Ala Glu Asp Asp 145 150 155 160 Met Thr Asn Phe Gln Arg Asn Phe Ser Thr Gly Glu Val Glu Val Glu                 165 170 175 Asp Ser Val Ile Tyr His Lys Thr Glu Phe Lys Glu Arg Arg Asn His             180 185 190 Tyr Ala Phe Tyr Ser Val Asn Ala Pro Ile Gln Gly Phe Asp Thr Asp         195 200 205 Arg Asp Lys Trp Lys Gly Leu Tyr Asn Asp Phe Asp Lys Pro Asp Ala     210 215 220 Val Phe Glu Gly Glu Pro Arg Asn Ser Glu Ala His Gly Trp Ser Pro 225 230 235 240 Ile Ala Ser His Tyr Leu Glu Val Glu Leu Ala Pro Gly Glu Ser Lys                 245 250 255 Asp Leu Ile Phe Val Leu Gly Tyr Ile Glu Val Ala Pro Glu Asn Lys             260 265 270 Trp Glu Ser Lys Gly Val Ile Asn Lys Ser Pro Ala Lys Glu Leu Ile         275 280 285 Ala Arg Phe Asp Ser Val Glu Lys Val Asp Ala Glu Leu Thr Lys Leu     290 295 300 Ala Asp Tyr Trp Ala Asn Leu Leu Ser Thr Tyr Ser Val Glu Ser Gly 305 310 315 320 Asp Glu Lys Leu Asp Arg Met Val Asn Ile Trp Asn Gln Tyr Gln Cys                 325 330 335 Met Val Thr Phe Asn Met Ser Arg Ser Ala Ser Phe Phe Glu Ser Gly             340 345 350 Ile Gly Arg Gly Met Gly Phe Arg Asp Ser Asn Gln Asp Leu Ile Gly         355 360 365 Phe Val His Gln Val Pro Glu Arg Ala Arg Glu Arg Ile Ile Asp Ile     370 375 380 Ala Ser Thr Gln Phe Glu Asp Gly Ser Ala Tyr His Gln Tyr Gln Pro 385 390 395 400 Leu Thr Lys Arg Gly Asn Asn Ala Ile Gly Gly Asn Phe Asn Asp Asp                 405 410 415 Pro Leu Trp Leu Ile Leu Ser Thr Thr Asp Tyr Ile Lys Glu Thr Gly             420 425 430 Asp Phe Ser Ile Leu Glu Glu Gln Val Pro Tyr Asp Asn Asp Ala Ser         435 440 445 Lys Ala Thr Ser His Phe Glu His Leu Lys Arg Ser Phe Tyr His Thr     450 455 460 Val Asn Asn Leu Gly Pro His Gly Leu Pro Leu Ile Gly Arg Ala Asp 465 470 475 480 Trp Asn Asp Cys Leu Asn Leu Asn Cys Phe Ser Glu Asp Pro Asn Glu                 485 490 495 Ser Phe Gln Thr Thr Gly Asn Lys Thr Gly Arg Thr Ala Glu Ser Leu             500 505 510 Met Ile Ala Gly Leu Phe Val Leu Tyr Gly Asn Glu Phe Val Lys Leu         515 520 525 Cys Arg Glu Ile Gly Gln Asp Gly Glu Ala Ala Glu Ala Gln Ala His     530 535 540 Ile Asp Gln Met Val Glu Ala Val Lys Lys His Gly Trp Asp Gly Glu 545 550 555 560 Trp Phe Leu Arg Ala Tyr Asp Tyr Tyr Gly Lys Lys Val Gly Ser Lys                 565 570 575 Glu Asn Glu Glu Gly Lys Ile Phe Ile Glu Ser Gln Gly Phe Cys Gly             580 585 590 Met Ala Gly Ile Gly Leu Glu Asp Gly Leu Val Glu Lys Ser Met Asp         595 600 605 Ser Val Lys Glu Trp Leu Asp Cys Asp Tyr Gly Ile Val Leu Gln Gln     610 615 620 Pro Ala Phe Thr Lys Tyr Tyr Ile Glu Tyr Gly Glu Ile Ser Thr Tyr 625 630 635 640 Pro Ala Gly Tyr Lys Glu Asn Ala Gly Ile Phe Cys His Asn Asn Pro                 645 650 655 Trp Ile Met Ile Thr Glu Thr Leu Leu Gly Arg Gly Asp Lys Ala Phe             660 665 670 Glu Tyr Tyr Arg Lys Ile Ala Pro Ala Tyr Leu Glu Glu Ile Ser Asp         675 680 685 Leu His Lys Val Glu Pro Tyr Ala Tyr Cys Gln Met Ile Ala Gly Lys     690 695 700 Asp Ala Tyr Leu Pro Gly Glu Gly Lys Asn Ser Trp Leu Thr Gly Thr 705 710 715 720 Ala Ser Trp Asn Phe Ala Ala Ile Thr Gln Tyr Ile Leu Gly Val Lys                 725 730 735 Pro Asp Tyr Ser Gly Leu Ala Ile Asn Pro Cys Ile Pro Ser Ser Trp             740 745 750 Asp Gly Phe Lys Val Thr Arg Lys Tyr Arg Gly Ala Thr Tyr Asn Ile         755 760 765 Ile Val Thr Asn Pro Thr His Val Ser Lys Gly Val Lys Ser Leu Thr     770 775 780 Leu Asn Gly Asn Ala Ile Asp Gly Tyr Ile Val Pro Pro Gln Gln Ala 785 790 795 800 Gly Thr Val Cys Asn Val Glu Val Thr Leu Gly                 805 810 <210> 46 <211> 2436 <212> DNA <213> Microbulbifer degradans <400> 46 atgaaatttg ggcactttga cgacaaagca cgcgagtatg taattaccga cccgaaaact 60 ccctacccgt ggataaacta cttaggcaac gaagacttct tcagcctagt atctaacact 120 gggggtggct acagttttta caaagatgca aagttccgtc gtttaacacg ctatagatac 180 aacaacgtac ccgtagacaa cggcggtaaa tatttttaca tcaatgatag tggcgatgta 240 tggagccccg gttggaagcc ggtaaaagca gagctagacg catacagctg cgctcacggc 300 cttagctaca cccgcattac cggcgaaaga aacggcattc aagcggaagt acttagcttt 360 atccctctcg gcacttgggc cgaaattcaa aaagttagcc ttaagaatac ctctggcgct 420 accaaaaaat ttaaactgtt ttctttcgcc gaatggtgcc tatggaacgc agaagatgac 480 atgaccaact tccaacgcaa cttctccacc ggtgaagtag aggtggaaga ctctgttatt 540 tatcacaaga cagaatttaa agagcgccgc aatcattacg cattctactc tgtaaacgca 600 ccaattcagg gcttcgacac cgacagagac aaatggaaag gcttgtacaa cgattttgat 660 aaacccgatg ccgtttttga aggcgagcct cgcaactccg aagcgcacgg ctggtcgcca 720 attgcatctc actatctaga agtggagctc gcaccaggcg aaagcaaaga cttaattttt 780 gtgcttggct atatagaagt tgccccagaa aacaaatggg aatcaaaggg cgttatcaac 840 aagtctccag ccaaagaact tattgcgcgt ttcgatagcg tagaaaaagt agatgccgag 900 ttaaccaagc tagccgatta ttgggcaaat ttgctttcta cttacagcgt agaaagtggc 960 gacgaaaagc tagaccgcat ggtaaatatt tggaaccaat accagtgtat ggtgacattt 1020 aatatgagtc gctctgcgtc tttcttcgaa tctggcattg gccgtggtat gggcttccgc 1080 gattccaatc aggatttgat aggctttgta caccaagtac ccgagcgcgc ccgcgaacgc 1140 ataattgata ttgcttctac tcagtttgaa gacggttcgg cctaccacca gtatcagcct 1200 ttaaccaaac gcggcaacaa cgcaattggc ggcaacttta acgatgaccc tctttggcta 1260 atcctttcta ccaccgatta cataaaagag actggcgatt tctctatttt agaagagcaa 1320 gtgccttacg ataatgatgc gagcaaagcc acaagtcatt ttgaacattt aaagcgctcg 1380 ttttatcaca cggttaataa tttaggccca catggcttgc cacttattgg tcgcgccgac 1440 tggaacgact gcctaaacct aaactgcttt agtgaagacc ctaacgaatc attccaaacc 1500 acgggcaaca aaaccggcag aacggctgag tcgttaatga ttgcaggttt atttgtttta 1560 tacggcaacg agtttgtaaa actgtgccgt gaaataggcc aagacggaga agcggcagaa 1620 gcccaagccc atattgacca aatggtagaa gctgtgaaaa agcacggctg ggatggcgag 1680 tggtttttgc gtgcttacga ctactacggt aaaaaagtag gcagtaaaga aaacgaagaa 1740 ggcaaaatat ttatcgaatc gcaaggtttc tgcggcatgg caggaatcgg cctagaagac 1800 ggccttgtcg aaaaatcgat ggattctgtt aaagaatggt tagattgcga ttacggtatt 1860 gtgttgcagc aaccggcgtt taccaagtac tacatagagt atggtgaaat ctccacctac 1920 cctgctggct acaaagagaa cgcaggtatc ttctgccaca acaacccgtg gattatgatc 1980 accgaaactt tgcttggccg cggtgacaaa gcctttgaat actaccgcaa aattgcacct 2040 gcatacctag aggaaattag cgatcttcac aaagtagagc cttacgccta ctgccagatg 2100 attgcaggta aagatgccta cttacctggc gagggtaaaa actcatggct aacagggacc 2160 gcttcgtgga acttcgctgc aattactcag tacattttag gcgtaaaacc agactatagc 2220 ggtttagcaa ttaacccttg cataccgtct agctgggatg gctttaaagt tacccgtaag 2280 tatcgcggcg caacctataa catcatcgta accaacccaa cccatgtaag caaaggcgta 2340 aaatcgctca ccctaaatgg caacgctatt gatggctaca tagtgccacc gcaacaagct 2400 ggcaccgtat gtaacgtaga agttacattg ggctaa 2436 <210> 47 <211> 788 <212> PRT <213> Microbulbifer degradans <400> 47 Met Leu Lys Ala Ile Asn Asn Gly Glu Arg Tyr Gln Leu Thr Ser Pro 1 5 10 15 Thr Ala Met Pro Gln Ser Ala Ser Phe Leu Trp Asn Lys Lys Met Met             20 25 30 Ile Gln Val Asn Cys Arg Gly Tyr Ala Val Ala Gln Phe Met Gln Pro         35 40 45 Glu Pro Ala Lys Tyr Ala Tyr Ala Pro Asn Leu Glu Ala Lys Thr Phe     50 55 60 Met Gln Pro Glu Gln Pro Tyr Tyr Ala His His Pro Gly Arg Phe Phe 65 70 75 80 Tyr Ile Lys Asp Glu Glu Thr Gly Glu Ile Phe Ser Ala Pro Tyr Glu                 85 90 95 Pro Val Arg Ser Gln Leu Asn Asn Phe Ser Phe Asn Ala Gly Lys Ser             100 105 110 Asp Ile Ser Trp His Ile Ala Ala Leu Gly Ile Glu Val Glu Leu Cys         115 120 125 Leu Ser Leu Pro Val Asp Asp Val Val Glu Leu Trp Glu Leu Lys Ile     130 135 140 Lys Asn Gly Gly Ala Gln Pro Arg Lys Leu Ser Ile Tyr Pro Tyr Phe 145 150 155 160 Pro Val Gly Tyr Met Ser Trp Met Asn Gln Ser Gly Asp Tyr Ser Gln                 165 170 175 Thr Ala Gly Gly Ile Ile Ala Ser Cys Val Thr Pro Tyr Gln Lys Val             180 185 190 Ala Asp Tyr Phe Lys Asn Lys Asp Phe Lys Asp Lys Thr Phe Phe Leu         195 200 205 His Glu Thr Ala Pro Ala Ala Trp Glu Val Asn Gln Lys Asn Phe Glu     210 215 220 Gly Glu Gly Gly Leu His Asn Pro Asn Ala Ile Gln Gln Glu Thr Leu 225 230 235 240 Gly Cys Gly Asn Ala Leu Tyr Glu Thr Pro Thr Ala Val Leu Gln Tyr                 245 250 255 Arg Arg Glu Leu Ala Ala Gln Glu Gln Gln Thr Phe Arg Phe Ile Phe             260 265 270 Gly Pro Ala Phe Asp Glu Ser Glu Ala Ile Ala Leu Arg Asn Lys Tyr         275 280 285 Leu Ser Ala Glu Gly Phe Ala Lys Ala Lys Ser Glu Tyr Gln Thr Tyr     290 295 300 Ile Thr Ser Gly Lys Gly Cys Leu Gln Ile Asn Thr Pro Asp Pro Glu 305 310 315 320 Leu Asn Asn Phe Val Asn His Trp Leu Pro Arg Gln Val Phe Tyr His                 325 330 335 Gly Asp Val Asn Arg Leu Thr Thr Asp Pro Gln Thr Arg Asn Tyr Ile             340 345 350 Gln Asp Asn Met Gly Met Ser Tyr Ile Lys Pro Asn Ile Thr Arg Gln         355 360 365 Ala Phe Leu His Ala Leu Ser Gln Gln Glu Glu Ser Gly Ala Met Pro     370 375 380 Asp Gly Ile Leu Leu Leu Glu Gly Ala Glu Leu Lys Tyr Ile Asn Gln 385 390 395 400 Ile Pro His Thr Asp His Cys Val Trp Leu Pro Val Cys Met Gln Ala                 405 410 415 Tyr Leu Asp Glu Thr Asn Asp Tyr Ala Leu Leu Asp Glu Ile Val Pro             420 425 430 Tyr Ala Ser Gly Glu Lys Arg Glu Thr Val Glu Gln His Met His His         435 440 445 Ala Met Arg Trp Leu Leu Gln Ala Arg Asp Glu Arg Gly Leu Ser Phe     450 455 460 Ile Ala Gln Gly Asp Trp Cys Asp Pro Met Asn Met Val Gly Tyr Lys 465 470 475 480 Gly Lys Gly Val Ser Gly Trp Leu Ser Val Ala Thr Ala Tyr Ala Leu                 485 490 495 Asn Leu Trp Ala Asp Val Cys Glu Gln Arg Gln Gln Asn Ser Cys Ala             500 505 510 Asn Glu Phe Arg Gln Gly Ala Lys Asp Ile Asn Ala Ala Val Asn Lys         515 520 525 His Ile Trp Asp Gly Glu Trp Phe Gly Arg Gly Ile Thr Asp Asp Gly     530 535 540 Val Leu Phe Gly Thr Ser Lys Asp Lys Glu Gly Arg Ile Phe Leu Asn 545 550 555 560 Pro Gln Ser Trp Ala Ile Leu Gly Gly Ala Ala Asp Glu Gln Lys Ile                 565 570 575 Pro Cys Leu Leu Asp Ala Val Glu Gln Gln Leu Glu Thr Pro Tyr Gly             580 585 590 Val Met Met Leu Ala Pro Ala Phe Thr Ala Met Arg Asp Asp Val Gly         595 600 605 Arg Val Thr Gln Lys Phe Pro Gly Ser Ala Glu Asn Gly Ser Val Tyr     610 615 620 Asn His Ala Ala Val Phe Tyr Ile Phe Ser Leu Leu Ser Ile Gly Glu 625 630 635 640 Ser Glu Arg Ala Tyr Lys Leu Leu Arg Gln Met Leu Pro Gly Pro Asp                 645 650 655 Glu Ala Asp Leu Leu Gln Arg Gly Gln Leu Pro Val Phe Ile Pro Asn             660 665 670 Tyr Tyr Arg Gly Ala Tyr Tyr Gln His Pro Arg Thr Ala Gly Arg Ser         675 680 685 Ser Gln Leu Phe Asn Thr Gly Thr Val Ser Trp Val Tyr Arg Cys Leu     690 695 700 Ile Glu Gly Val Phe Gly Leu Lys Gly Ser Pro Gln Gly Leu Val Val 705 710 715 720 Gln Pro Gln Leu Pro Val Ala Trp Gln Thr Ala Glu Ala Val Arg Glu                 725 730 735 Phe Arg Gly Ala Thr Phe Asn Val Ser Tyr Arg Lys Ser Ser Asp Ile             740 745 750 Lys Glu Met Glu Ile Gln Leu Asn Glu Ser Val Ile Ser Gly Asn Thr         755 760 765 Ile Ser Asp Ile Thr Ala Gly Ala Thr Tyr Gln Leu Thr Val Leu Leu     770 775 780 Pro Ala Thr His 785 <210> 48 <211> 2367 <212> DNA <213> Microbulbifer degradans <400> 48 atgttaaaag ccattaacaa cggcgaacgc tatcaactca ctagccctac cgctatgccg 60 caaagcgcat cgtttttatg gaataaaaaa atgatgatac aagtaaattg ccgcggctac 120 gccgttgcgc aatttatgca gccagaacca gccaaatacg cttacgcacc caatctggaa 180 gcaaaaacat ttatgcaacc agagcaaccc tattacgcgc atcaccccgg gcgctttttc 240 tatataaaag atgaagagac aggcgagatt ttttcggcac cctacgagcc tgtgcgcagc 300 cagctgaaca actttagctt taacgcaggc aagagcgata taagctggca tattgccgct 360 ttaggcattg aagtagagct atgtcttagc ctgccggtgg acgatgtagt agaattgtgg 420 gaactaaaaa taaaaaacgg cggcgcgcaa cctcgtaaac tcagtattta cccgtacttt 480 cctgtgggtt acatgtcgtg gatgaatcaa tctggtgact acagccaaac cgccggcggc 540 attattgcca gctgcgtaac gccttatcaa aaagtcgccg actactttaa gaataaagac 600 tttaaagata aaacgttctt tcttcacgaa accgccccag cagcatggga agtaaaccag 660 aaaaacttcg aaggcgaagg cgggttgcac aaccccaacg ccatacaaca agaaacgctg 720 ggctgcggca acgcattgta cgaaacgccc acagcggtat tgcaataccg ccgcgaactt 780 gcagcgcaag agcagcaaac ctttcgcttt atttttggcc cagcatttga cgagagcgaa 840 gccattgcac tgcgcaataa gtatttatct gccgaaggtt ttgccaaagc aaaaagcgaa 900 taccaaacct atataacgag cggcaaaggc tgcttgcaaa ttaacacccc agacccagaa 960 ctaaacaact ttgtaaacca ctggctaccg cgccaagtgt tttatcacgg cgatgtaaac 1020 cggttaacca ccgacccgca aacgcgcaat tatattcaag acaatatggg catgagctac 1080 attaagccca acattacgcg gcaggcgttt ttacatgcct taagccagca ggaagaaagc 1140 ggtgcaatgc ccgacggcat tttattgctt gaaggcgccg agcttaaata cataaaccaa 1200 ataccccata ccgatcactg cgtttggctg ccggtgtgta tgcaagccta tttggatgaa 1260 accaatgact acgccctatt agacgaaata gtaccctatg cgagtggcga gaagcgcgaa 1320 actgttgagc aacatatgca tcacgctatg cgctggcttt tgcaagcacg cgacgaacgc 1380 ggcctaagct ttatcgcaca gggcgactgg tgcgacccca tgaacatggt gggctacaag 1440 ggcaaagggg tatccggctg gctttcagtc gctaccgctt atgcattaaa cctgtgggca 1500 gatgtttgcg aacaacggca gcaaaacagt tgcgccaacg aatttagaca gggcgctaaa 1560 gatataaacg cggcggtaaa caagcatatt tgggatggcg aatggtttgg ccgcggcatt 1620 acagatgacg gcgtactgtt tggcaccagc aaagataaag aaggcagaat ttttctaaac 1680 ccacaaagct gggcaatact tggcggcgcc gccgacgaac aaaaaatccc atgcctgcta 1740 gacgcagtag agcaacaact ggaaacccct tacggcgtaa tgatgctggc ccccgcgttt 1800 accgccatgc gcgatgacgt aggccgagtt acccaaaaat tcccaggctc tgcagaaaac 1860 ggctctgttt ataatcacgc ggcggtgttt tatatattta gcttgttatc cattggcgag 1920 agcgaacgcg catataaact gctacgccaa atgctgcctg ggccagatga agccgatctt 1980 ttacagcgcg gccaactgcc agtattcata cctaactatt atcgcggcgc atactaccag 2040 cacccccgca ccgccggtcg ctctagccag ctctttaata cgggtacagt ctcgtgggtt 2100 taccgctgct taattgaagg ggtattcggc ttgaaaggct cgccacaagg cttagttgta 2160 caaccgcaac tgcctgtcgc ctggcaaaca gcagaagccg ttagggaatt tagaggcgca 2220 acgtttaacg tgagctaccg caaaagcagc gatataaaag aaatggaaat acagctaaat 2280 gaatcggtaa taagtggcaa caccatctcc gacatcaccg ccggcgcgac ctatcaatta 2340 accgttctat tacctgccac acactaa 2367 <210> 49 <211> 574 <212> PRT <213> Microbulbifer degradans <400> 49 Met Lys Lys Leu Ile Lys Pro Thr Leu Ser Trp Val Ala Gly Val Ala 1 5 10 15 Leu Ser Leu Gly Ile Ala Gln Gly Ala Gly Ala Gln Asn Val Gln Phe             20 25 30 Val Gly Asn Ile Thr Thr Asn Gly Ser Val Arg Asn Asp Phe Met Asp         35 40 45 Tyr Trp Asp Gln Ile Thr Pro Glu Asn Glu Gly Lys Trp Gly Ser Val     50 55 60 Glu Arg Ser Arg Asp Asn Tyr Ser Trp Ser Gly Gln Asp Ala Ala Tyr 65 70 75 80 Asn Phe Ala Arg Ala Asn Gly Ile Pro Phe Lys Ala His Thr Leu Val                 85 90 95 Trp Gly Ser Gln Tyr Pro Ser Trp Ile Asn Asn Leu Ser Asn Ala Glu             100 105 110 Lys Ala Ala Glu Ile Glu Glu Trp Ile Arg Asp Tyr Cys Asn Arg Tyr         115 120 125 Pro Ala Thr Asp Ile Ile Asp Val Val Asn Glu Ala Thr Pro Gly His     130 135 140 Ala Pro Ala Asn Tyr Ala Arg Asp Ala Phe Gly Asp Asn Trp Ile Ile 145 150 155 160 Lys Ser Phe Gln Leu Ala Arg Gln Tyr Cys Pro Asn Ala Thr Leu Val                 165 170 175 Leu Asn Asp Tyr Asn Val Leu Ile Trp Asn Thr Asn Asp Phe Ile Ala             180 185 190 Met Ala Gln Pro Val Ile Asn Ala Gly Val Val Asp Ala Leu Gly Leu         195 200 205 Gln Ala His Gly Leu Glu Ser Leu Ser Ala Ser Gln Leu Lys Ser Thr     210 215 220 Leu Asp Arg Ile Ala Asn Leu Gly Leu Pro Ile Tyr Ile Ser Glu Tyr 225 230 235 240 Asp Val Arg Ser Thr Asn Asp Gln Glu Gln Leu Arg Ile Met Arg Asp                 245 250 255 Gln Phe Pro Val Phe Tyr Asn His Pro Ser Val Arg Gly Ile Thr Leu             260 265 270 Trp Gly Tyr Met Val Gly Ala Thr Trp Arg Glu Gly Thr Gly Leu Ile         275 280 285 Arg Ala Asp Gly Ser His Arg Pro Ala Met Thr Trp Leu Met Asn Tyr     290 295 300 Leu Glu Asn Asn Arg Gly Gly Ser Thr Ser Ser Ser Ser Ser Ser Ser 305 310 315 320 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly Ser Ser Ser Gly                 325 330 335 Gly Pro Ser Ser Leu Thr Val Glu Leu Glu Ser Leu Ser Asp Ser Ser             340 345 350 Asn Phe Ser Pro Phe Ser Val Gln Ser Asp Ser Ser Ala Ala Gly Gly         355 360 365 Gln Tyr Val Val Trp Pro Asn Asn Gly Asn Gln Ile Val Ser Ser Pro     370 375 380 Ser Asp Ser Ala Ser Gly Gln Ile Gln Val His Phe Thr Leu Ser Gln 385 390 395 400 Ser Ala Asp Val Gln Phe Gln Ile Arg Ala Asp Leu Ala Asn Gly Asn                 405 410 415 Asp Asp Ser Phe Tyr Tyr Lys Leu Asp Ser Gly Ser Trp Asn Thr Gln             420 425 430 Asn Asn Ala Ser Thr Ser Gly Trp Gly Thr Leu Thr Pro Ala Thr Phe         435 440 445 Ser Asn Val Ser Thr Gly Ser His Thr Leu His Ile Leu Arg Arg Glu     450 455 460 Asp Gly Ala Lys Leu Asp Lys Val Thr Leu Asn Ala Ser Val Gly Gln 465 470 475 480 Val Ser Ala Ser Thr Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser                 485 490 495 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly Ala Ala Val Ala             500 505 510 Ser Cys Asp Gly Val Asn Glu Tyr Pro Ser Trp Thr Ala Lys Asp Trp         515 520 525 Ser Gly Gly Asp Tyr Asn His Ala Asn Ser Gly Asp Tyr Met Ser Tyr     530 535 540 Gln Gly Val Leu Tyr Arg Ala Asn Trp Tyr Thr Ala Thr Val Pro Gly 545 550 555 560 Ser Asp Ser Ser Trp Thr Arg Val Gly Asp Cys Asn Phe Val                 565 570 <210> 50 <211> 1725 <212> DNA <213> Microbulbifer degradans <400> 50 atgaagaagt taattaagcc tacgctatcg tgggttgcag gagttgcttt gtcgctgggt 60 attgcccagg gagctggtgc tcaaaatgtg cagtttgttg gtaatatcac taccaatggt 120 agtgtgcgca acgacttcat ggactactgg gatcagatta ccccagagaa tgaaggcaag 180 tggggctcgg tggagcgcag tcgcgacaac tattcatgga gcggccaaga tgccgcctac 240 aattttgccc gtgccaacgg catcccattt aaagcacata ctttagtatg gggcagtcaa 300 tatcccagct ggataaacaa tttaagtaac gcggaaaaag ccgctgagat tgaagagtgg 360 attcgcgatt actgtaaccg ttacccagcc accgatatta tcgatgttgt caatgaagca 420 acgccgggcc acgcgccagc aaattatgct cgcgatgcat ttggcgacaa ctggataatc 480 aagtccttcc agctggcacg tcagtactgc cccaatgcca cgttagtgtt gaacgactac 540 aacgtactta tttggaacac caatgatttt atagcgatgg cccagccggt aattaacgcc 600 ggagtagtag atgctttggg tttgcaggcc cacggtctgg agagcctttc tgcgtcgcaa 660 ttaaaatcga ctctggatcg tatcgccaat ttgggtttgc caatttatat ctctgaatac 720 gatgttcgca gcaccaatga tcaggagcag ctgcgtatta tgcgtgatca attccctgta 780 ttttacaacc acccaagtgt acgtggcata actttgtggg gttatatggt gggggccacc 840 tggcgagaag gcacaggttt gattcgtgct gatggctccc atcgtccagc gatgacctgg 900 ttgatgaact atctggagaa caatcgtggc ggctcaacct cttcaagtag ttcatcctcc 960 tctagcagtt cgtcttccag tagttcttct tcgggaagtt cctctggtgg cccaagtagt 1020 ttgacggtag agctagaatc tttgtcggat agcagtaact tttcgccatt ctcggtacag 1080 agtgacagca gcgcagcggg cggccagtac gtggtatggc ctaacaacgg caatcagatt 1140 gtaagctcac cctccgatag cgccagcggg caaattcagg tgcactttac cctgtcgcaa 1200 tcggcggatg tgcaatttca gattcgtgca gacctagcta acggcaatga cgactctttt 1260 tattacaagc tggactcagg ctcttggaat actcagaaca acgcttccac gtctggttgg 1320 ggcaccttaa ccccagcaac tttctctaat gtatccacag gatcccatac cttacacatt 1380 ctccgcagag aagatggggc gaaactcgat aaggtaactc tgaatgcttc agttggtcag 1440 gtttccgcta gtacaggcag tagctccagc tcttccagca gctccagttc atccagcagt 1500 tctagttctt caagcagcag cggcgcggca gtcgcaagtt gtgacggtgt taatgaatac 1560 cccagctgga cagcaaaaga ttggtctggg ggtgactata accacgccaa tagcggtgac 1620 tacatgagct atcagggtgt tctatatcga gcaaactggt acaccgcaac tgttcctgga 1680 agtgattctt cctggactcg agttggcgat tgcaattttg tgtaa 1725 <210> 51 <211> 619 <212> PRT <213> Microbulbifer degradans <400> 51 Met Ile Lys Leu Arg Gln Ser Ile His Gly Ala Leu Ala Arg Thr Val 1 5 10 15 Gly Ile Ile Ser Ile Ser Thr Gly Leu Val Leu Ala Ala Gln Thr Ala             20 25 30 Ser Ala Ala Cys Glu Tyr Thr Val Thr Asn Ser Trp Gly Ser Gly Phe         35 40 45 Thr Ala Ser Ile Arg Ile Thr Asn Asp Thr Gly Ser Ala Val Asn Gly     50 55 60 Trp Ala Val Asn Trp Gln Tyr Ala Asn Gly Asn Arg Val Thr Asn Ser 65 70 75 80 Trp Asn Ala Thr Leu Ser Gly Asn Asn Pro Tyr Ser Ala Ser Asn Ile                 85 90 95 Gly Trp Asn Gly Gly Ile Gln Pro Gly Gln Ser Val Glu Phe Gly Phe             100 105 110 Gln Gly Thr Ala Asn Gly Ala Ala Glu Thr Pro Ala Val Thr Gly Ala         115 120 125 Val Cys Ala Thr Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser     130 135 140 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 145 150 155 160 Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly                 165 170 175 Ala Asn Cys Val Glu Met Cys Lys Trp Tyr Gln Asp Ala Pro Arg Pro             180 185 190 Leu Cys Asn Asn Gln Asn Ser Gly Trp Gly Trp Glu Asn Asn Gln Ser         195 200 205 Cys Ile Gly Arg Ala Thr Cys Glu Ser Gln Pro Ser Asn Ala Gly Gly     210 215 220 Val Val Asn Ser Cys Pro Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 225 230 235 240 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser                 245 250 255 Ser Thr Ser Ser Ser Ser Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser             260 265 270 Ser Ser Ser Ser Gly Ser Ala Ala Asn Leu Tyr Thr Leu Ala Asp Phe         275 280 285 Pro Ile Gly Val Ala Val Thr Ala Gly Asn Glu Ser Arg Ser Phe Leu     290 295 300 Ser Ile Ala Ala Lys Glu Ala Thr Val Lys Lys His Phe Asp Gln Ile 305 310 315 320 Thr Ala Gly Asn Ile Met Lys Met Ser Tyr Leu His Pro Ser Glu Asn                 325 330 335 Ser Tyr Thr Phe Ser Gln Ala Asp Ala Met Val Asn Trp Ala Asn Ser             340 345 350 Asn Gly Val Ser Val His Gly His Thr Phe Ile Trp His Ser Asp Tyr         355 360 365 Gln Val Pro Asn Trp Met Asn Asn Tyr Ser Gly Asn Phe Ala Ser Met     370 375 380 Met Asp Thr His Val Thr Thr Ile Ala Asp His Phe Glu Gly Arg Val 385 390 395 400 Val Ser Trp Asp Val Val Asn Glu Ala Ile Asp Glu Ser Gln Ser Ser                 405 410 415 Cys Tyr Arg Asn Ser Leu Phe Tyr Gln Arg Leu Gly Lys Ala Tyr Ile             420 425 430 Ala Asn Ala Phe Arg Ala Ala Arg Ala Ala Asp Pro Ser Val Glu Leu         435 440 445 Tyr Tyr Asn Asp Tyr Asp Thr Glu Gly Gly Asn Ala Asn Lys Leu Asn     450 455 460 Cys Leu Leu Gln Leu Val Asp Asp Leu Gln Ala Asn Asn Val Pro Ile 465 470 475 480 Asp Gly Val Gly Phe Gln Met His Val Gln Ile Asp Trp Pro Ser Thr                 485 490 495 Ser Asn Ile Ala Ala Ala Phe Gln Ala Ile Val Asp Arg Gly Leu Lys             500 505 510 Val Lys Ile Thr Glu Leu Asp Val Pro Ile Asn Asn Pro Tyr Gly Ser         515 520 525 Gly Ser Phe Pro Gln Tyr Ser Thr Tyr Thr Ser Gln Ala Ala Ala Leu     530 535 540 Gln Lys Ala Arg Tyr Lys Ser Ile Val Lys Thr Tyr Leu Thr Val Val 545 550 555 560 Pro Ala His Leu Arg Gly Gly Leu Thr Val Trp Gly Ile Trp Asp Gly                 565 570 575 Asp Ser Trp Leu Leu Asp Phe Asp Asn Arg Gln Gly Ala Asp Asp Trp             580 585 590 Pro Leu Leu Phe Ser Gly Pro Ala Asn Gly Pro Tyr Val Glu Lys Glu         595 600 605 Ala Phe Tyr Gly Val Ala Glu Ala Leu Thr Glu     610 615 <210> 52 <211> 50 <212> PRT <213> Microbulbifer degradans <400> 52 Met Leu Asp Glu Leu Leu Asp Glu Leu Glu Leu Leu Leu Glu Leu Glu 1 5 10 15 Leu Leu Asp Glu Glu Leu Glu Leu Glu Leu Glu Glu Leu Asp Pro Val             20 25 30 Ala His Thr Ala Pro Val Thr Ala Gly Val Ser Ala Ala Pro Leu Ala         35 40 45 Val pro     50 <210> 53 <211> 103 <212> PRT <213> Microbulbifer degradans <400> 53 Met Gly Lys Ser Ala Lys Val Tyr Lys Phe Ala Ala Glu Pro Leu Glu 1 5 10 15 Leu Glu Leu Leu Glu Glu Val Glu Leu Val Leu Asp Glu Leu Glu Leu             20 25 30 Val Asp Glu Leu Glu Leu Leu Asp Glu Leu Glu Leu Leu Asp Glu Leu         35 40 45 Glu Leu Glu Glu Leu Glu Glu Leu Leu Glu Leu Asp Gly Gln Leu Phe     50 55 60 Thr Thr Pro Pro Ala Leu Asp Gly Cys Asp Ser His Val Ala Arg Pro 65 70 75 80 Ile Gln Leu Trp Leu Phe Ser Gln Pro Gln Pro Leu Phe Trp Leu Leu                 85 90 95 His Lys Gly Arg Gly Ala Ser             100 <210> 54 <211> 1860 <212> DNA <213> Microbulbifer degradans <400> 54 atgatcaagc tacgtcaatc tatccacggc gccttggcgc gtaccgtggg cataataagt 60 ataagcaccg gacttgtact cgcagcgcaa actgcaagtg cagcctgtga atacaccgta 120 accaattcgt ggggttcggg ttttaccgcg agtattcgca taacaaacga taccggtagc 180 gcagtaaacg gttgggcggt taactggcaa tacgctaatg gcaaccgtgt aacaaattca 240 tggaacgcta cgctgtctgg caataaccct tatagcgcca gcaatattgg ttggaacggc 300 ggtattcaac ctgggcagtc ggtggaattt ggttttcaag gcacggctaa tggcgcggca 360 gaaacaccag cggtaacggg ggctgtatgt gctacagggt ctagctcttc cagctcaagc 420 tctagttctt cgtctagcag ctctagttct agcagcagtt cgagttcatc gagtagctcg 480 tctagcactt caagctcgtc atctagcagc tcttccagtt catcgggcgc aaactgtgta 540 gaaatgtgta agtggtatca agatgcgcct cgccctttat gcaataacca aaatagtggt 600 tggggttggg aaaacaacca aagttgtatc ggccgagcaa cgtgcgaatc gcaaccgtct 660 aatgcgggtg gggtggtaaa tagttgtccg tctagttcta gcagctcttc aagttcctct 720 agttcgagct cgtctagcag ttcaagctcg tctagcagtt caagctcgtc tactagttct 780 agttcatcaa gcacaagttc tacttcttca agtagttcta gctctagcgg ttctgctgca 840 aacttatata ccttggcaga tttccccatt ggcgttgctg taactgcggg taatgagagc 900 cgtagctttt tatctattgc tgcgaaagag gcaactgtta aaaaacactt cgaccaaatt 960 acagccggta acattatgaa gatgagttac ttgcacccat ccgaaaatag ctacaccttt 1020 agtcaagcgg atgccatggt taactgggca aatagcaacg gcgtaagtgt gcacggccat 1080 acttttattt ggcattccga ttaccaagta ccaaattgga tgaataatta cagcggtaat 1140 tttgcgtcta tgatggatac ccacgtaacc actattgccg atcattttga aggccgagta 1200 gtaagctggg atgtggtaaa cgaagctatc gatgagagcc aatctagttg ttatcgcaac 1260 tctttgtttt accagcgttt aggtaaagct tatattgcca atgcgttccg cgcggcccga 1320 gcagcagacc ctagcgtaga gttgtattac aacgattacg ataccgaagg tggcaatgcc 1380 aataagttaa attgcttgtt gcaattagtc gatgacttgc aagcgaacaa tgtgcctatc 1440 gatggtgtgg gctttcaaat gcacgtgcaa attgattggc ccagcaccag caatattgct 1500 gcggctttcc aagctattgt ggatcgcggc ttaaaggtaa aaattactga gctggatgtg 1560 cctattaata acccttatgg cagtggttca ttcccgcaat attcaactta cacgtcacaa 1620 gccgctgcgt tgcaaaaggc gcgttataaa tccattgtaa aaacctactt gactgttgtg 1680 ccagcgcatt tgcgcggggg cttaaccgta tggggtatat gggatggtga tagctggttg 1740 ttagattttg ataatcgtca aggcgctgat gattggccgc tattatttag tggcccagct 1800 aatggcccct atgtagaaaa agaagcattc tatggcgtgg cagaggcgct tacagaatag 1860 <210> 55 <211> 470 <212> PRT <213> Microbulbifer degradans <220> <221> MOD_RES <222> (301) .. (457) Variable amino acid <400> 55 Met Asn Cys Thr Arg Arg Asn Ile Val Lys Ala Gly Leu Leu Gly Ser 1 5 10 15 Ala Phe Val Ala Leu Pro Ala Val Ala Arg Ala Leu Pro Gly Leu Ala             20 25 30 Thr Lys Phe Arg Asp Gln Phe Tyr Val Gly Thr Ala Val Ser Ala Arg         35 40 45 Ser Leu Asn Thr Pro Ser Gly Ala Phe Ala Ala Thr Val Ala His Gln     50 55 60 Phe Asn Ala Leu Thr Ala Glu Asn Ala Met Lys Pro Ala Leu Leu Gln 65 70 75 80 Pro Gln Met Gly Glu Trp Arg Trp Gln Asp Ala Asp Ala Ile Val Arg                 85 90 95 Phe Ala Glu Gln His Gln Met Leu Met His Gly His Thr Leu Val Trp             100 105 110 His Ser Gln Thr Pro Asp Trp Phe Phe Gln Asn Lys Gln Gly Glu Pro         115 120 125 Ala Asp Lys Ala Thr Leu Tyr Arg Arg Gln Glu Glu Tyr Ile Asn Ala     130 135 140 Val Val Gly Arg Tyr Lys Gly Arg Val His Ser Trp Asp Val Val Asn 145 150 155 160 Glu Ala Glu Asp Glu Gly Lys Gly Trp Arg Lys Ser His Trp Tyr Asn                 165 170 175 Ile Cys Gly Pro Glu Phe Met Glu Arg Ala Phe Arg Leu Ala His Ala             180 185 190 Ala Asp Pro Lys Ala His Leu Cys Tyr Asn Asp Tyr Asn Met His Leu         195 200 205 Pro Gln Lys Arg Glu Phe Leu Val Lys Leu Phe Lys Asp Tyr Ile Lys     210 215 220 Arg Gly Val Pro Ile His Gly Val Gly Leu Gln Gly His Val Gly Leu 225 230 235 240 Asp Tyr Pro Ser Leu Asp Glu Leu Glu Lys Thr Ile Val Ala Met Ala                 245 250 255 Asp Leu Gly Leu Lys Val His Ile Thr Glu Leu Asp Val Asp Val Leu             260 265 270 Pro Ala Pro Trp Gln Leu Ala Ser Ala Asp Ile Ser Thr Lys Phe Glu         275 280 285 Tyr Asp Lys Ser Leu Asn Pro Tyr Val Asp Gly Leu Xaa Xaa Xaa Xaa     290 295 300 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 305 310 315 320 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa                 325 330 335 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa             340 345 350 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa         355 360 365 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa     370 375 380 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 385 390 395 400 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa                 405 410 415 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa             420 425 430 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa         435 440 445 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Thr Met Tyr Lys Met Arg Leu     450 455 460 Pro Ala Lys Ile Leu Ala 465 470 <210> 56 <211> 1413 <212> DNA <213> Microbulbifer degradans <220> <221> modified_base (902) (902). (1369) <223> a, c, g, or t <400> 56 gtgaattgta cgcgtaggaa tatagtaaaa gcaggccttc ttggctcggc attcgtcgcc 60 ctgcctgccg tggcgcgcgc gctgcctgga ttggccacga aatttcgcga tcagttttac 120 gtgggcactg cggttagtgc gcgctcactt aatacgccca gcggcgcgtt tgcagccact 180 gtcgcgcatc aattcaatgc actaaccgct gaaaacgcca tgaagcccgc cttacttcaa 240 ccacaaatgg gggagtggcg ctggcaggat gccgatgcca ttgtgagatt tgccgagcag 300 catcagatgc taatgcatgg tcacaccctt gtgtggcatt cgcaaacgcc agattggttc 360 ttccaaaaca agcagggcga accggcagac aaagcaaccc tataccgcag gcaagaggag 420 tatatcaatg ccgtagttgg gcgctataaa gggcgggtac actcgtggga tgtggtgaat 480 gaagcagaag atgagggtaa aggctggcgc aagagccact ggtataacat ttgtgggcca 540 gagtttatgg aacgagcctt tcgcttagct cacgcagcgg acccaaaagc acacttatgt 600 tacaacgatt acaatatgca cttgccgcaa aagcgcgaat ttttggttaa gttattcaaa 660 gactacatta agcgcggcgt gcctattcac ggcgtagggt tgcaggggca tgtgggctta 720 gactacccct cgctggacga gttggaaaaa accatcgtgg ccatggccga tttaggtcta 780 aaagtacaca ttacagaatt ggatgtagat gtattacccg cgccatggca actagctagc 840 gcagatataa gtactaaatt cgagtacgac aaaagcttaa acccgtacgt tgatggtttg 900 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 960 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1020 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1080 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1140 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1200 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1260 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1320 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gactatgtac 1380 aaaatgcgct tgcccgcgaa aatattagct taa 1413 <210> 57 <211> 1186 <212> PRT <213> Microbulbifer degradans <400> 57 Met Leu Arg Ser Thr Gln Ser Thr Pro Ile Val Lys Arg Lys Ile Ser   1 5 10 15 Ala Tyr Val Gly Trp Gly Leu Cys Val Leu Leu Ser Val Cys Thr Ala              20 25 30 Ser Ile Ser Trp Ala Gly Asn Pro Ile Val Ser His Val Tyr Thr Ala          35 40 45 Asp Pro Ala Ala Arg Val Ile Asn Gly Arg Ala Tyr Val Met Val Thr      50 55 60 His Asp Gln Asp Asn Gln Asn Asp Tyr Gly Gly Leu Ile Asp Tyr Tyr  65 70 75 80 Leu Phe Ser Ser Asp Asp Met Val Asn Trp Gln Asp His Gly Ile Val                  85 90 95 Trp Asn Ser Arg Thr Asp Ser Ser Trp Ala Ser Leu Ala Tyr Ala Pro             100 105 110 Asp Phe Ile Glu Arg Asn Gly Lys Tyr Tyr Leu Tyr Phe Pro Asn Gly         115 120 125 Ala Asn Ser Ile Gly Val Ala Val Ala Asp Ser Pro Glu Gly Pro Tyr     130 135 140 Thr Asp Pro Leu Gly Arg Pro Leu Val Asp Arg Asn Thr Pro Asn Ala 145 150 155 160 Asn Val Asp Trp Leu Phe Asp Pro Gly Val Phe Ile Asp Asp Asp Gly                 165 170 175 Gln Ala Phe Leu Tyr Phe Gly Gly Gly Ala Asp Gly Thr Ala Arg Val             180 185 190 Ile Arg Leu Asn Asn Asp Met Ile Ser Thr Ser Gly Ala Ala Ile Ser         195 200 205 Ile Asp Val Pro Asn Phe Phe Glu Ala Leu Tyr Met His Lys Arg Asn     210 215 220 Gly Ile Tyr Tyr Leu Ser Tyr Ser Thr Asn Pro Ser Ala Gly Met Ser 225 230 235 240 Ile Asp Tyr Met Thr Ser Asn Asn Pro Thr Ser Gly Phe Thr His Arg                 245 250 255 Gly Thr Ile Leu Pro Asn Pro Trp Glu Asn Asn Ser Asn Asn Asn His             260 265 270 Gln Ser Ile Ile Glu Phe Asn Asn Glu Trp Tyr Ile Phe Tyr His Asn         275 280 285 Arg Ala Val Ala Asn Thr Arg Gly Asp Ser Thr Phe Ser Arg Ser Ile     290 295 300 Asn Val Asp Arg Leu Tyr Tyr Asn Ser Asp Gly Ser Ile Arg Glu Val 305 310 315 320 Asn Ala Ser Ser Ile Gly Val Pro Ala Val Arg Asn Val Asn Ala Phe                 325 330 335 Ser Ile Asn Gln Ala Glu Thr Phe Asp Gln Glu Gly Gly Ile Glu Thr             340 345 350 Glu Pro Ser Ser Glu Gly Thr Leu Asn Ile Gln Met Gly Pro Gly Asp         355 360 365 Trp Val Lys Val Ala Asn Val Asp Phe Gly Asn Gly Ala Thr Gln Phe     370 375 380 Asn Ala Arg Val Ala Ser Ala Ile Asp Asn Ser Lys Leu Glu Ile Ile 385 390 395 400 Leu Gly Ser Leu Ser Asn Thr Pro His Ala Ser Leu Glu Ile Thr Asn                 405 410 415 Thr Gly Gly Trp Gln Asn Trp Gln Thr Gln Ser Thr Ser Phe Asn Ala             420 425 430 Ile Thr Gly Val His Asp Val Tyr Leu Arg Gly Thr Ser Gly His Asn         435 440 445 Leu Asn Trp Phe Glu Phe Glu Gly Glu Asn Asn Gly Gly Ser Ser Gln     450 455 460 Leu Thr Val Glu Leu Glu Asp Leu Ala Ser Gln Ser Leu Phe Ala Pro 465 470 475 480 Leu Ser Val Arg Ser Asp Asn Met Ala Asn Asn Gly Ala Tyr Ile Glu                 485 490 495 Trp Ser Asn Asp Gly Ser Asn Gln Ile Leu Ser Val Ala Ser Glu Gln             500 505 510 Ser Gln Gly Gln Ile Ser Val Pro Phe Thr Leu Ser Gln Ala Ser Asp         515 520 525 Val Glu Phe Asn Val Arg Val Asn Leu Ala Asn Gly Asn Asp Asp Ser     530 535 540 Phe Tyr Tyr Lys Leu Asn Ser Asn Ser Trp Gln Thr Phe Asn Asn Gln 545 550 555 560 Ala Thr Thr Gly Trp Gln Val Leu Thr Pro Asn Thr Phe Thr Gly Leu                 565 570 575 Ser Pro Gly Asn His Ile Leu Thr Leu Leu Arg Arg Glu Asp Gly Ala             580 585 590 Lys Leu Asp Thr Leu Thr Leu Val Ala Ser Ala Gly Ser Ile Gln Thr         595 600 605 Asn Asn Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Thr Thr Ser     610 615 620 Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Gly Ala Ala Pro Thr Gly 625 630 635 640 Asn Val Thr Tyr Ser Ile Asn Val Thr Asn Asp Trp Gln Ser Gly Tyr                 645 650 655 Cys Ala Glu Leu Thr Val Thr Asn Asn Thr Asn Asn Ala Leu Gln Trp             660 665 670 Gln Ala Ser Val Ser Met Ser Asp Ser Val Asp Ser Met Trp Asn Ala         675 680 685 Ser Trp Ser Gln Ser Gly Asn Ile Leu Asn Val Ser Gly Val Glu Trp     690 695 700 Asn Asn Thr Leu Gln Ala Gly Gln Ser Gln Ser Gly Ile Gly Phe Cys 705 710 715 720 Ala Thr Arg Ala Ser Ser Ser Ser Ser Ser Ser Thr Thr Ser Ser Thr                 725 730 735 Ser Gly Ser Thr Ser Ser Ser Ser Ser Ser Gly Gly Tyr Thr Val Pro             740 745 750 Ser Asn Asn Phe Ala Val Asn Gly Gly Val Glu Asn Asn Leu Gln Ser         755 760 765 Trp Gly Ala Thr Ala Gly Ser Val Thr Arg Ser Thr Glu Gln Arg Tyr     770 775 780 Ser Gly Asn Ala Ser Ala Arg Ile Thr Asn Arg Ala Glu Asn Trp His 785 790 795 800 Gly Leu Thr Phe Ser Val Gly Glu Leu Thr Gln Gly Asn Leu Tyr Glu                 805 810 815 Val Ala Val Trp Val Lys Leu Ala Ala Gly Ser Ala Asp Thr Pro Ile             820 825 830 Thr Leu Thr Ala Lys Arg Gln Asn Asp Ser Asp Asp Ser Thr Tyr Asn         835 840 845 Glu Tyr Thr Gly Ile Val Thr Thr Ile Ala Asn Asp Ser Glu Trp Val     850 855 860 Leu Leu His Gly Gln Tyr Thr Gln Thr Gly Thr Ala Phe Glu His Phe 865 870 875 880 Ile Ile Glu Ser Glu Ser Asp Ser Val Ser Phe Tyr Ala Asp Glu Phe                 885 890 895 Ser Ile Gly Gly Glu Val Thr Pro Lys Asn Glu Val Gly Phe Phe Val             900 905 910 Gly Asn Ile Thr Thr Asn Gly Asn Val Arg Asn Asp Phe Thr Gln Tyr         915 920 925 Trp Asp Gln Leu Thr Pro Glu Asn Glu Gly Lys Trp Gly Ser Val Glu     930 935 940 Arg Thr Arg Asp Val Tyr Asp Trp Ser Gly Leu Asp Arg Ala Tyr Asn 945 950 955 960 Tyr Ala Lys Gln Asn Asn Ile Pro Phe Lys Gln His Thr Met Val Trp                 965 970 975 Gly Ser Gln Gln Pro Asn Trp Ile Asp Ser Leu Ser Pro Ala Glu Gln             980 985 990 Ala Ala Glu Ile Glu Glu Trp Ile Arg Asp Tyr Cys Ala Arg Tyr Pro         995 1000 1005 Asp Thr Glu Met Ile Asp Val Val Asn Glu Ala Thr Leu Gly His Ala    1010 1015 1020 Pro Ala Asn Tyr Ala Ala Ser Ala Phe Gly Asn Asn Trp Ile Ile Arg 1025 1030 1035 1040 Ser Phe Glu Leu Thr Arg Gln Tyr Cys Pro Asn Ser Ile Leu Ile Leu                1045 1050 1055 Asn Asp Tyr Asn Val Leu Ser Trp Asn Thr Gln Glu Phe Ile Gln Met            1060 1065 1070 Ala Thr Pro Ala Val Asn Ala Gly Val Val Asp Ala Ile Gly Leu Gln        1075 1080 1085 Ala His Gly Leu Ala Asp Trp Ser Leu Ser Asp Leu Glu Thr Lys Leu    1090 1095 1100 Asn Gln Val Ala Ala Leu Gly Leu Pro Ile Tyr Ile Ser Glu Tyr Asp 1105 1110 1115 1120 Ile Glu Lys Thr Asn Asp Gln Glu Gln Leu Arg Val Met Gln Thr Gln                1125 1130 1135 Phe Pro Leu Phe Tyr Asn His Pro Ser Val Lys Gly Ile Thr Ile Trp            1140 1145 1150 Gly Tyr Val Val Gly Ala Thr Trp Arg Asp Gly Thr Gly Leu Leu His        1155 1160 1165 Ser Asn Gly Thr Pro Arg Pro Ala Leu Thr Trp Leu Met Asp Tyr Leu    1170 1175 1180 Asn arg 1185 <210> 58 <211> 3561 <212> DNA <213> Microbulbifer degradans <400> 58 atgttgcgaa gcacccaatc aacacccatt gttaagcgaa agatttctgc ctatgtaggt 60 tggggtctgt gcgtgttact tagcgtctgc acggcctcga tctcttgggc aggtaaccct 120 attgtgtctc atgtatatac cgcagaccct gctgcacggg taataaacgg aagagcctat 180 gtaatggtta cccacgatca ggataaccaa aatgattacg gtggtttgat tgattactac 240 ctgttctcat cggacgatat ggttaattgg caagatcacg gtattgtgtg gaattctcga 300 acagacagta gttgggccag tcttgcttac gccccagatt ttatcgagcg caatggaaag 360 tactacctgt actttcccaa cggcgcaaac tctattggtg tcgctgtggc cgatagccct 420 gagggcccct atactgatcc actcggtagg ccgctggttg accgcaatac ccccaatgcc 480 aatgttgact ggctgttcga tcccggtgta tttattgatg acgacggaca agcctttttg 540 tactttggtg gaggcgctga tggaaccgcg cgcgttattc gtttaaataa cgacatgata 600 agtaccagtg gtgcagccat aagtattgac gtacctaact tctttgaagc gctatacatg 660 cataagcgca acggcattta ctacttatcc tactcgacca accccagcgc ggggatgagc 720 atagattaca tgacgagtaa taaccctacc tcagggttca cccatcgcgg caccattttg 780 cccaaccctt gggaaaataa ttccaataac aaccaccagt caattattga atttaataac 840 gaatggtaca ttttttacca caatagagct gtcgcaaata cgcggggcga tagtaccttt 900 tcccgctcta ttaacgtgga tcgtctttac tacaattccg acggcagtat tcgagaagta 960 aatgccagtt caataggtgt acccgcggta cgtaatgtta atgctttttc cataaaccaa 1020 gcagaaacat tcgatcaaga aggtggcata gaaactgagc cgtcttctga aggtaccttg 1080 aatattcaga tgggcccagg agattgggta aaagttgcta acgtcgattt tggtaacggc 1140 gccacacaat ttaacgctcg agttgctagc gcaatcgata attcaaagct ggaaattatt 1200 ttaggcagtc tcagtaatac cccgcatgcc tcgctcgaaa ttaccaacac aggcgggtgg 1260 caaaattggc aaacacaaag cacaagtttt aatgcaataa ctggtgttca cgatgtatac 1320 ctgcgcggta cttctgggca caacctaaat tggtttgaat ttgaaggcga aaataatgga 1380 ggaagcagtc agctaacggt tgagttggaa gacttggctt cgcaatctct ttttgctccc 1440 cttagcgtac gctccgataa catggctaat aacggcgctt acattgaatg gagtaatgat 1500 gggagcaatc agattctcag tgtggccagc gagcaatcgc aaggccaaat cagtgtccca 1560 tttactctat cgcaagcttc cgatgtcgaa tttaacgtac gcgtgaatct tgctaatggc 1620 aatgatgatt cgttttatta caagctaaac agtaatagct ggcagacttt taataatcaa 1680 gctaccactg gttggcaggt gctcacgccc aacaccttca ctggtcttag ccctgggaat 1740 cacattctta ctctacttcg gcgtgaagat ggcgccaaat tagataccct cacgttggta 1800 gcctccgcgg gcagtattca aaccaataac agctcatcaa gttctacctc cagcagtagt 1860 tcaacgacta gctcaagttc aaccagctcg agtagttcct ctggcgccgc gccaactggt 1920 aacgttactt actctataaa tgttactaac gactggcaaa gtggttattg tgcggagctt 1980 accgttacca ataacacgaa caacgctctg cagtggcaag ctagtgtttc tatgagcgat 2040 agtgtcgaca gtatgtggaa tgctagctgg tcgcagagcg ggaacatact taacgtaagc 2100 ggggtagagt ggaataatac gttgcaagca gggcaaagcc agagtggcat aggattttgt 2160 gctacacgtg ccagctcgtc ttcctccagc tctacaacaa gttctacttc cggttccaca 2220 tcaagctcta gttcatcggg aggctatacc gttccgagta ataatttcgc agtcaatggt 2280 ggtgtagaaa acaacctgca gagctggggc gcaacggcgg gttcagtaac acgttctact 2340 gaacaacgtt atagcggaaa cgcaagcgcg cgtataacaa atcgagcaga aaactggcac 2400 ggtttgacgt tcagtgttgg tgagcttacg caaggcaacc tgtacgaagt tgcggtgtgg 2460 gtaaaacttg cggcaggcag tgcggacaca cctattacgc ttaccgccaa acgacaaaat 2520 gatagcgacg attccactta taacgaatat accggcatag tcacgaccat tgctaacgat 2580 tctgaatggg tgctgctgca cgggcaatac actcaaactg gcacagcgtt tgagcatttt 2640 attatcgagt cagaaagcga tagcgtaagt ttttatgccg atgagttttc tattggtgga 2700 gaggtcacgc ccaaaaacga agtgggattt tttgtgggta acattaccac taatggcaat 2760 gtgcgcaatg attttactca gtactgggat caactaacac cagaaaatga aggaaagtgg 2820 ggttcggtag aacgcactcg tgatgtgtat gattggagtg gactagacag agcctataac 2880 tacgccaaac aaaataatat tccgtttaaa cagcatacta tggtgtgggg tagccaacag 2940 cccaactgga ttgattcgct cagcccagca gaacaggctg cagagataga ggagtggata 3000 agagattatt gtgcgcgcta tcctgatact gaaatgattg atgtggtaaa cgaagcaacg 3060 ctgggccatg ctcctgctaa ctacgcggcg agtgcgtttg gcaataattg gatcattcgt 3120 tcgttcgagc ttactcgtca atattgtcct aacagcattt taatattgaa cgattacaat 3180 gttttaagtt ggaacactca agagtttatc cagatggcta ctccggctgt caatgcaggc 3240 gttgtagatg caattggatt acaagcacac ggcttagcgg attggtcttt aagtgattta 3300 gaaaccaaac taaaccaggt tgcggcattg ggtttaccca tttatatatc cgaatacgat 3360 atagaaaaaa ctaacgacca agaacagctg cgcgtaatgc aaactcagtt cccgctgttt 3420 tataaccatc catcggtgaa aggcattact atttgggggt atgttgttgg ggctacttgg 3480 cgcgatggga cgggattgtt gcacagtaac ggaacaccca gaccggcact tacttggtta 3540 atggattact tgaatagata g 3561 <210> 59 <211> 670 <212> PRT <213> Microbulbifer degradans <400> 59 Met Val Ile Ile Thr Met Lys Ala Gly Leu Leu Leu Arg Ile Leu Leu 1 5 10 15 Thr Val Leu Ala Leu Asn Met Leu Ala Ala Cys Gly Gly Ser Ser Ser             20 25 30 Asn Thr Lys Glu Pro Val Thr Gln Pro Glu Pro Glu Pro Glu Gln Gln         35 40 45 Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu     50 55 60 Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Met Glu Pro Gln 65 70 75 80 Pro Glu Pro Gln Ala Pro Pro Ala Gly Gly Val Ser Ile Ile Asp Thr                 85 90 95 Asn Pro Asn Asn Ala Ser Phe Trp Ala Gly Ser Asn Asn Gly Asp Val             100 105 110 Gly Ser Arg Ala Val Ile Asp Val Asp His Pro Glu Phe Ser Gln Ala         115 120 125 Thr Arg Ile Thr Val Ser Asn Pro Ala Ser Asp Tyr Trp Asn Gly Gln     130 135 140 Leu Ser Phe Pro Leu Asn Ala Ser Val Ala Ala Gly Asp Val Val Leu 145 150 155 160 Val Arg Leu Tyr Met Arg Ser Val Glu Asn Thr Tyr Glu Ser Gly Ala                 165 170 175 Ser Phe Thr Thr Val Phe Ile Glu Asp Asn Ile Asp Phe Thr Lys Phe             180 185 190 Leu Asn Arg Glu Ile Thr Ala Ala Gln Asp Trp Val Glu Tyr Tyr Leu         195 200 205 Pro Ala Glu Ile Thr Asp Asn His Ala Thr Gly Glu Val Gly Leu Arg     210 215 220 Ile Gly Phe Gly Ala Gly Pro Arg Ala Gln Val Phe Asp Ile Gly Gly 225 230 235 240 Val Glu Leu Leu His Tyr Thr Asn Thr Asp Ile Ser Ala Met Pro Ser                 245 250 255 Thr Arg Pro Ser Tyr Glu Gly Arg Glu Pro Asp Ala Ala Trp Arg Thr             260 265 270 Ala Ala Ala Glu Arg Ile Glu Gln His Arg Lys Gly Asp Phe Glu Leu         275 280 285 Thr Val Val Asp Asp Gly Asn Pro Ile Ala Asn Ala Thr Ile Asp Val     290 295 300 Asp Phe Gln Lys His Ala Tyr His Phe Gly Ser Val Thr Val Gly His 305 310 315 320 Leu Leu Met Gly Thr Ser Glu Asp Ser Ala Ile Tyr Arg Glu Lys Val                 325 330 335 Leu Glu Leu Phe Asn Gln Ser Gly Pro Glu Asn Asp Leu Lys Trp Gly             340 345 350 Pro Trp Glu Gly Glu Trp Gly Asn Asn Phe Asn Gln Thr Gln Thr Leu         355 360 365 Asn Gly Leu Gln Trp Leu Arg Asp Asn Gly Leu Tyr Thr Arg Gly His     370 375 380 Val Met Val Trp Pro Ser Lys Arg Asn Leu Pro Asn Leu Met Gln Gln 385 390 395 400 Tyr Leu Pro Glu Gly Asp Pro Ala Ser Ala Asn Pro Glu Ala Lys Gln                 405 410 415 Val Val Leu Asp His Ile Asp Asp Ile Ala Thr Ala Thr Ala Asn Tyr             420 425 430 Leu Asp Glu Trp Asp Val Leu Asn Glu Pro Tyr Asp Asn His Tyr Leu         435 440 445 Met Asp Ala Phe Gly Asp Ser Val Met Val Asp Trp Phe Asn Arg Ala     450 455 460 Arg Thr Asn Leu Pro Ala His Gly Leu Tyr Ile Asn Asp Tyr Ser Ile 465 470 475 480 Leu Ser Ala Gly Gly Arg Asn Phe Ala His Gln Glu His Tyr Thr Asn                 485 490 495 Thr Ile Gln Tyr Leu Val Asp Asn Asn Ala Pro Ile Thr Gly Ile Gly             500 505 510 Leu Gln Ser His Phe Gly Asp Ser Pro Thr Ala Ile Thr Arg Ile Tyr         515 520 525 Glu Ile Ile Asp Gln Tyr Ser Thr Ala Phe Pro Gln Leu Asp Ile Arg     530 535 540 Ala Thr Glu Phe Asp Val Ser Thr Thr Asp Glu Asp Leu Gln Ala Asp 545 550 555 560 Phe Thr Arg Asp Phe Leu Thr Ile Phe Phe Ser His Pro Lys Thr Val                 565 570 575 Gly Val Gln Leu Trp Gly Phe Trp Ala Asn Ala His Trp Tyr Pro Asn             580 585 590 Ala Ala Leu Tyr Asp Ala Asp Trp Arg Glu Lys Pro Asn Ala Leu Ala         595 600 605 Trp Lys Glu Gln Ile Phe Asn Glu Trp Trp Asn Asp Phe Asp Gly Thr     610 615 620 Thr Asn Ala Gln Gly Lys Phe Asp Glu Arg Gly Phe Tyr Gly Asp Tyr 625 630 635 640 Gln Val Thr Val Thr Val Gly Glu Glu Gln Gln Ile Phe Thr Phe Ser                 645 650 655 Leu Val Lys Gly Gly Glu Gln Asn Phe Ser Phe Glu Trp Gln             660 665 670 <210> 60 <211> 2013 <212> DNA <213> Microbulbifer degradans <400> 60 atggtcataa taactatgaa agccggttta cttctacgca tcctattaac tgtactcgcg 60 ctcaatatgc ttgccgcatg tggcggtagt tctagcaata ccaaagaacc cgttacccag 120 ccggaaccag agccagagca gcagccagaa ccagaaccag agccagagcc agagccagag 180 ccagaaccag agccagaacc agagccagaa ccagagccag agcctgaaat ggaaccgcag 240 ccagagccac aagcgccgcc tgcaggtggt gtatctatca ttgataccaa ccccaacaat 300 gcatcgtttt gggcaggctc aaacaatggt gatgtgggca gtagggctgt tatagatgtc 360 gatcaccccg aatttagcca agcgacgcgc ataaccgtaa gcaaccccgc tagcgactat 420 tggaatggtc agctctcctt cccgcttaat gcgagtgtgg cggcggggga tgtagtatta 480 gtgcgtttgt acatgcgctc ggtggagaat acttacgaat cgggtgctag ttttactacc 540 gtatttattg aagacaacat cgactttact aaatttttaa accgcgaaat aaccgccgcg 600 caagattggg tagagtatta cctacccgca gaaattaccg ataaccatgc aaccggtgaa 660 gtgggcttgc gcattggctt tggcgctggc cctagggcgc aggtgtttga tattggcggt 720 gtagagctat tgcattacac caatactgat ataagcgcta tgcctagtac acgcccaagt 780 tacgaaggcc gcgagccaga tgccgcatgg cgtacagcgg cggcagagcg aattgagcag 840 caccgcaaag gcgactttga gctaacagta gtggacgatg gcaaccctat cgccaatgcc 900 accatagatg tagattttca aaaacacgcc tatcattttg gctcggtaac tgttggccat 960 ctattgatgg gcaccagtga agatagcgcc atttaccgcg aaaaagtgct cgagctattt 1020 aaccaaagtg gcccagaaaa cgatttaaag tggggcccat gggaaggcga gtggggcaac 1080 aattttaacc aaactcaaac cctaaacggc ttgcagtggc tgcgcgataa cggcctgtac 1140 acacgtggcc atgtaatggt ttggccttct aagcgcaact tgccaaactt aatgcagcaa 1200 tatttaccag aaggcgaccc cgccagcgcc aacccagaag caaaacaagt ggtgctggat 1260 cacatcgatg atatagcaac cgcaacagct aattatttag atgagtggga tgtactaaac 1320 gagccttacg acaaccacta tttaatggat gcctttggcg atagtgtaat ggtggattgg 1380 tttaatcgcg cgcgtactaa cctgcctgcg cacggtttgt acataaacga ttacagtatt 1440 ttatctgcgg gcgggcgcaa ttttgctcac caagaacact acaccaacac gattcaatat 1500 ttggtcgata acaacgcacc catcaccggt ataggtttgc aaagtcactt tggcgactcg 1560 cctacagcca ttacgcgtat ttacgaaatt attgatcaat acagtaccgc gtttccgcag 1620 ttagatattc gcgcaacgga atttgacgta agtacaacag atgaagacct gcaggcagat 1680 tttacccgcg acttcttaac gatattcttt agccacccta aaacagtggg tgtgcagttg 1740 tggggttttt gggcaaatgc acattggtac cctaatgcag cgctttatga tgccgattgg 1800 cgagaaaagc ccaatgcact agcttggaaa gagcaaattt ttaacgagtg gtggaacgac 1860 tttgacggca cgaccaacgc acagggtaaa tttgatgaac gcggttttta cggcgattac 1920 caagtaactg taaccgtagg tgaagagcag caaattttta cctttagcct agttaaaggc 1980 ggcgaacaaa actttagttt tgagtggcaa tag 2013 <210> 61 <211> 275 <212> PRT <213> Microbulbifer degradans <400> 61 Met Asn Ile Lys Thr Phe Phe Pro Ala Leu Ile Ala Ser Val Phe Leu 1 5 10 15 Leu Ile Asn Ala Ser Thr Gly Tyr Ala Ala Ser Ile Thr Lys Thr Leu             20 25 30 Cys Asn Pro Ala Asp Ser Asp Asn Gly Tyr Gly Ala Gly Thr Phe Asn         35 40 45 Gly Lys Phe Tyr Ser Trp Phe Glu Leu Ser Gln Glu Asp Ile Thr Asp     50 55 60 Cys Asp Thr Lys Ile Gly Phe Tyr Asn Glu Thr Asn Arg His Phe Arg 65 70 75 80 Val Glu Trp Asn Val Ala Gln Ser Trp Gly Glu Asp Ala Ile Gly Gly                 85 90 95 Met Gly Trp Ser Ser Gly Ser Arg Asp Arg Lys Ile Gly Tyr Asn Val             100 105 110 Gly Gln Leu Thr Thr Asn Ser Ser Ile Gln Lys Ala Leu Val Ala Met         115 120 125 Tyr Gly Trp Ser Cys Ser Thr Ser Gly Gly Asn Gln Ile Ser Gln Glu     130 135 140 Tyr Tyr Val Val Asp Thr Trp Asp Gly Gly Lys Phe Val Pro Trp Asp 145 150 155 160 Glu Asn Ala Asn Asn Gly Asn Gly Ala Pro Ala Gln Ser Val Gly Thr                 165 170 175 Val Ser Ala Asn Gly Ala Thr Tyr Asp Val Tyr Lys Val Arg Arg Asn             180 185 190 Gly Ala Gln Tyr Cys Phe Asn Gly Ser Ser Arg Ser Phe Asp Gln Phe         195 200 205 Trp Ser Val Arg Arg Thr Pro Arg Ala Ile Asn Gly Asn Arg Asn Met     210 215 220 Asp Phe Arg Pro His Ala Asn Arg Trp Asp Asn Ser Asp Leu Gly Phe 225 230 235 240 Lys Val Asp Gly Leu Ser Ser Gly Tyr Gln Ile Leu Ala Val Glu Ile                 245 250 255 Phe Gly Asp Ala Asn Leu Arg His Lys Gly Ala Ala Asp Ile Thr Leu             260 265 270 Trp Pro Arg         275 <210> 62 <211> 828 <212> DNA <213> Microbulbifer degradans <400> 62 atgaacataa aaacattctt ccccgcactt attgcaagtg tatttttatt aattaacgcc 60 agcactggct atgcagcaag cattaccaaa acgctttgca acccagccga ttccgataac 120 ggctacggtg caggaacctt caatggcaaa ttttattctt ggtttgagtt aagccaagaa 180 gacattaccg attgcgatac aaaaattggt ttttacaacg aaaccaatcg acactttagg 240 gtggagtgga atgttgctca atcttgggga gaagatgcaa ttggtggaat gggttggagc 300 tctggctcga gagatagaaa aataggttac aacgttggcc aacttacaac taattcttct 360 attcaaaaag cattggttgc tatgtatggc tggtcttgct ctaccagtgg tggcaaccaa 420 atatcacaag aatattatgt agtggataca tgggacggcg gcaagtttgt gccttgggat 480 gaaaacgcaa ataatggcaa cggtgctcca gcacagagtg taggaacagt tagcgctaat 540 ggtgcaacat acgatgttta taaggttcgc cgcaacggtg cgcaatattg ttttaatggc 600 agcagccgct cgtttgatca gttttggagt gtgcgtagaa cgcctagagc gattaacggc 660 aaccgtaata tggattttcg cccgcacgcc aaccgctggg acaacagtga cctaggtttt 720 aaagttgacg ggttaagcag cggttaccaa attttagcgg ttgaaatatt tggtgatgcg 780 aacctaagac ataaaggtgc agcagatatt actttatggc cacgctaa 828 <210> 63 <211> 767 <212> PRT <213> Microbulbifer degradans <400> 63 Met Lys Ser Ile Asn Val Cys Gly Arg Arg Leu Lys Gln Ala Leu Ala 1 5 10 15 Ala Ile Ala Thr Ala Ala Ala Thr Leu Trp Phe Thr Pro Val Asp Ala             20 25 30 Gln Thr Leu Thr Ser Asn Gln Thr Gly Thr His Gly Gly Tyr Tyr Tyr         35 40 45 Ser Phe Trp Thr Asp Ser Ala Gly Thr Val Ser Met Thr Leu Gly Asn     50 55 60 Gly Gly Asn Tyr Ser Ser Ser Trp Ser Asn Thr Gly Asn Trp Val Gly 65 70 75 80 Gly Lys Gly Trp Gln Thr Gly Gly Arg Lys Thr Val Asn Tyr Ser Gly                 85 90 95 Thr Phe Asn Pro Ser Gly Asn Gly Tyr Leu Thr Leu Tyr Gly Trp Thr             100 105 110 Gln Asn Pro Leu Ile Glu Tyr Tyr Ile Ile Glu Ser Trp Gly Thr Tyr         115 120 125 Arg Pro Gly Glu Ser Gly Thr Tyr Tyr Gly Thr Val Asn Thr Asp Gly     130 135 140 Gly Thr Tyr Asp Ile Tyr Arg Thr Gln Arg Val Asn Gln Pro Ser Ile 145 150 155 160 Glu Gly Thr Ala Thr Phe Tyr Gln Tyr Trp Ser Val Arg Gln Gln Lys                 165 170 175 Arg Val Gly Gly Thr Ile Thr Thr Gly Asn His Phe Asp Ala Trp Ala             180 185 190 Ser His Gly Leu Asn Leu Gly Thr His Asn Tyr Met Val Met Ala Thr         195 200 205 Glu Gly Tyr Gln Ser Ser Gly Asn Ser Asn Ile Thr Val Ser Glu Gly     210 215 220 Ser Gly Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Thr Gly Gly Pro 225 230 235 240 Ser Gly Thr Asn Ile Val Val Arg Ala Gln Gly Val Ser Gly Gln Glu                 245 250 255 His Ile Asn Leu Ile Ile Gly Gly Asn Val Val Ala Asp Trp Thr Leu             260 265 270 Ser Thr Ser Met Gln Asp Tyr Thr Tyr Thr Gly Asn Ala Ala Gly Asp         275 280 285 Leu Gln Val Glu Tyr Asp Asn Asp Ala Ser Gly Arg Asp Val Glu Leu     290 295 300 Asp Tyr Val Tyr Val Asn Gly Glu Ile Arg Gln Ala Glu Asp Met Glu 305 310 315 320 Tyr Asn Thr Ala Thr Tyr Ser Gly Glu Cys Gly Gly Gly Ser Tyr Ser                 325 330 335 Gln Thr Met His Cys Ser Gly Val Ile Gly Phe Gly Asp Thr Ser Asp             340 345 350 Cys Phe Ser Gly Asn Cys Asn Gly Ala Ser Ser Thr Ser Ser Ser Ser         355 360 365 Ser Ser Ser Ser Thr Ser Ser Ser Thr Ser Ser Gly Gly Asn Asn Asn     370 375 380 Ser Gly Ile Thr Val Arg Ala Arg Gly Thr Asn Gly Asp Glu His Ile 385 390 395 400 Asn Leu Ile Val Gly Gly Asn Ile Val Gly Asn Trp Thr Leu Thr Thr                 405 410 415 Ser Asn Gln Asn Tyr Val Tyr Asn Gly Asn Ala Ser Gly Asp Val Glu             420 425 430 Val Gln Phe Asp Asn Asp Ala Asn Gly Arg Asp Val Ile Leu Asp Tyr         435 440 445 Val Ile Val Asn Gly Glu Thr Arg Gln Ala Glu Asp Met Glu Tyr Asn     450 455 460 Thr Ala Thr Tyr Ser Gly Ser Cys Gly Gly Gly Ser Tyr Ser Glu Thr 465 470 475 480 Met His Cys Ser Gly Glu Ile Gly Phe Gly His Thr Asp Asp Cys Phe                 485 490 495 Ser Gly Asn Cys Thr Ser Ser Ser Gly Thr Thr Gly Ser Ser Gly Gly             500 505 510 Thr Ser Ser Asn Asn Gly Thr Ser Ser Cys Asn Gly Tyr Val Gly Ile         515 520 525 Thr Phe Asp Asp Gly Pro Gly Asn Asn Thr Ala Thr Leu Ile Asn Leu     530 535 540 Leu Gln Gln Asn Asn Leu Thr Pro Val Thr Trp Phe Asn Thr Gly Gln 545 550 555 560 Asn Ile Ala Ala Asn Thr Gly Gln Phe Ala Gln Gln Lys Ser Val Gly                 565 570 575 Glu Ile Gln Asn His Ser Tyr Thr His Ser His Met Leu Asn Trp Ser             580 585 590 Tyr Gln Gln Val Arg Asp Glu Leu Ala Ser Thr Asn Gln Ala Ile Val         595 600 605 Asn Ala Gly Gly Ala Thr Pro Thr Leu Phe Arg Pro Pro Tyr Gly Glu     610 615 620 Thr Asn Ser Thr Ile Asn Gln Ala Ala Gln Asp Leu Gly Leu Arg Val 625 630 635 640 Ile Thr Trp Asp Val Asp Ser Arg Asp Trp Asp Gly Ala Ser Ala Ser                 645 650 655 Ala Ile Ala Asn Ser Ala Asn Gln Leu Gln Asn Gly Gln Val Ile Leu             660 665 670 Met His Asp Ala Ser Tyr Asn Asn Thr Asn Gly Ala Ile Ser Gln Phe         675 680 685 Ala Ala Asn Leu Arg Ala Arg Gly Leu Cys Ala Gly Lys Ile Asp Pro     690 695 700 Ser Thr Gly Arg Ala Val Ala Pro Ser Thr Asn Thr Gly Gly Asn Thr 705 710 715 720 Gly Ser Asn Thr Gly Asn Gly Gly Asn Gly Gly Met Cys Asn Trp Tyr                 725 730 735 Gly Thr Ser Ile Pro Leu Cys Gln Thr Thr Asn Asp Gly Trp Gly Trp             740 745 750 Glu Asn Ser Gln Ser Cys Val Ser Gln Asn Thr Cys Asn Ser Gln         755 760 765 <210> 64 <211> 2304 <212> DNA <213> Microbulbifer degradans <400> 64 atgaagtcaa tcaatgtatg cggcagacgc ctcaagcaag ccctcgcagc aatagcaacc 60 gctgcagcaa ctctctggtt tacgccagtg gatgcacaaa ccttaacctc aaaccaaact 120 ggtactcatg gtggttacta ctattccttc tggaccgaca gtgctggcac tgtttctatg 180 acactcggca atggcggcaa ttacagttca tcgtggagca ataccggtaa ctgggtggga 240 ggtaaaggct ggcaaacggg gggacgcaaa accgtaaact attccggtac gtttaacccc 300 tcgggcaatg gttatttaac cctctacggt tggacccaaa acccactcat tgaatactac 360 atcattgaaa gctggggcac ctatcgccca ggtgaaagcg gaacctacta cggcaccgtc 420 aacaccgatg gcggcactta cgatatttat cgcacccaac gcgttaacca accgtcaatt 480 gaaggcactg caacgtttta tcagtactgg agtgttaggc aacaaaaacg cgtaggcggc 540 accataacaa ccggcaacca ttttgatgcg tgggcgagtc atggccttaa cctaggcaca 600 cacaattaca tggtaatggc caccgaaggt tatcaaagta gcggcaactc caatattacc 660 gttagcgaag gcagcggttc gagcagtact agttcgagta gctctagcac cggtggccca 720 agtggtacca atattgttgt gcgcgcacaa ggtgtaagcg gccaagaaca tatcaattta 780 attattggcg gtaacgtagt ggcagactgg acgctttcaa ccagcatgca agattacacc 840 tacaccggta atgccgcagg cgacctgcaa gtagaatacg acaacgatgc tagtggtcgc 900 gatgtagagc tagactatgt gtatgtgaat ggcgaaattc gtcaagcaga agacatggaa 960 tacaacaccg caacttacag tggtgaatgt ggtggcggtt cctattcgca aaccatgcac 1020 tgcagcggtg taattggctt tggcgatacc agtgattgtt ttagcggcaa ctgtaatggt 1080 gcatcttcta caagttctag ttcgtctagt agctcaacca gctctagcac aagctctggc 1140 ggtaacaata acagcggcat tactgttcgc gcacgcggta ccaatggcga tgaacatatc 1200 aaccttattg ttggcggcaa tatagtaggc aattggacgc tcaccaccag caaccaaaat 1260 tatgtttaca acggcaatgc atctggtgat gtagaagtac aattcgacaa cgatgccaac 1320 ggtcgcgatg ttattctcga ttacgtaata gtaaatggcg aaactcgcca agcggaagat 1380 atggaataca acacggcgac ctacagcggt tcctgtggtg gtggctccta ttcggaaaca 1440 atgcactgca gcggcgaaat tggttttggt cacaccgacg attgctttag tggaaattgc 1500 actagcagca gcggcacaac cggtagctct ggaggaacat caagcaataa cggtacaagt 1560 agctgtaacg gttatgtagg tattaccttc gatgatggcc caggcaataa caccgctaca 1620 ttaataaact tactacaaca aaataactta accccagtaa cttggtttaa cacaggccaa 1680 aatattgctg ccaatacagg tcagtttgcc cagcaaaaaa gtgttggtga aattcaaaac 1740 cacagctaca cccattccca tatgcttaat tggagctatc aacaagttcg cgacgaactc 1800 gccagcacca atcaagctat tgtgaatgct gggggcgcaa cgccaactct attccgtccg 1860 ccttatggcg aaacaaactc caccattaat caagcggcac aagatttagg cctgcgcgta 1920 ataacctggg atgtagattc gcgcgattgg gatggcgcaa gcgcttcagc tattgccaac 1980 tcggctaatc agttgcaaaa cggccaagta attttgatgc acgatgccag ctacaacaat 2040 accaacggag ccatatcaca atttgcagcc aatctaagag caagagggct atgtgcaggt 2100 aaaatagacc caagcactgg ccgcgcagtt gcaccaagca caaataccgg cggcaacact 2160 ggcagcaata caggaaatgg cggtaatggc ggcatgtgta actggtacgg caccagcatt 2220 ccattatgcc aaactaccaa cgacggttgg ggctgggaaa actcacaaag ctgcgtttcg 2280 caaaatacct gtaactcaca ataa 2304 <210> 65 <211> 360 <212> PRT <213> Microbulbifer degradans <400> 65 Met Cys Leu Lys Ile Asn Arg Cys Trp Val Phe Val Trp Leu Cys Ile 1 5 10 15 Cys Ala Thr Thr Ala His Ser Glu Thr Tyr Val Pro Ala Asp Asn Asp             20 25 30 Gln Tyr Leu Tyr Thr Gly Arg Ile Asp Phe Ser Asp Ile Lys Ala Pro         35 40 45 Ser Leu Ser Trp Pro Gly Thr Ser Ile Lys Ala Asn Phe Thr Gly Glu     50 55 60 His Leu Glu Val Val Leu Asp Asp Gln Asn Gly Lys Asn Phe Phe Asn 65 70 75 80 Val Ile Ile Asp Gly Asn Asp Arg Phe Pro Tyr Val Leu Glu Ala Lys                 85 90 95 Gln Gly Glu His Arg Tyr Leu Ile Ser Ser Ala Leu Ser Lys Gly Lys             100 105 110 His Ser Val Glu Ile Tyr Lys Arg Thr Glu Gly Glu Glu Gly Ala Thr         115 120 125 Leu Phe Lys Gly Leu Trp Leu Ala Asp Asp Ser Tyr Leu Leu Lys Pro     130 135 140 Pro Lys Arg Pro Lys Arg Arg Ile Glu Ile Tyr Gly Asp Ser Ile Thr 145 150 155 160 Ser Gly Met Gly Asn Glu Gly Ala Asp Asn Gly Ala Asp His Leu Gly                 165 170 175 Ser Glu Lys Asn Asn Tyr Leu Ala Tyr Gly Ala Ile Thr Ala Arg Asn             180 185 190 Leu Asn Ala Glu Leu His Thr Ile Ser Gln Ser Gly Ile Gly Val Met         195 200 205 Val Ser Trp Phe Pro Phe Ile Met Pro Gln Phe Tyr Asn Gln Leu Ser     210 215 220 Ala Val Gly Asn Asn Asp Ser Ile Trp Asp Phe Lys Gln Trp Thr Pro 225 230 235 240 His Val Val Val Ile Asn Leu Met Gln Asn Asp Ser Trp Leu Ile Asp                 245 250 255 Arg Glu Lys Arg Leu Thr Pro Ile Pro Ala Asp Ala Gln Arg Ile Ala             260 265 270 His Tyr Gln Ala Phe Val Gln Ser Ile Arg Ala Glu Tyr Pro Lys Ala         275 280 285 Gln Ile Ile Cys Ala Leu Gly Ser Met Asp Ala Thr Ala Asn Glu Lys     290 295 300 Trp Pro Asn Tyr Val Arg Glu Ala Val Lys Asn Met Gln Asp Asn Gly 305 310 315 320 Asp Asn Lys Ile Asp Thr Ile Phe Phe Glu Tyr Ile Gly Tyr Gly Gln                 325 330 335 His Pro Arg Val Ala Gln His Asn Ala Asn Ala Asp Lys Leu Thr Lys             340 345 350 Phe Ile Lys Lys Lys Met Lys Trp         355 360 <210> 66 <211> 1083 <212> DNA <213> Microbulbifer degradans <400> 66 atgtgcctaa aaataaaccg gtgctgggtg tttgtttggt tgtgtatttg cgcaactact 60 gcccatagtg aaacctacgt acccgcagat aacgaccaat acctttatac cggccgtata 120 gattttagcg atataaaagc accctcgcta agctggcccg gcacaagtat aaaagccaac 180 tttaccggcg aacatttaga ggtagtgtta gacgatcaaa acggtaagaa tttttttaat 240 gtgattatcg acggtaacga tcgatttcct tatgtgctag aagctaaaca aggtgagcat 300 cgatatttaa tttcttctgc gctaagcaag ggcaagcaca gcgtagaaat ttataaacgt 360 acagaaggcg aagagggcgc aacgctattt aaagggcttt ggttagccga tgatagttat 420 ttattaaaac cccctaaacg cccaaaacgc agaatagaaa tttatggtga ctcaattaca 480 agcggtatgg gtaacgaagg cgcagataac ggcgccgacc atttgggctc cgaaaaaaat 540 aattaccttg cctatggggc tattaccgca cgcaatttaa acgccgagct acataccatt 600 tcgcaaagcg gtattggggt aatggtaagt tggtttccgt ttattatgcc gcagttttac 660 aaccagctaa gtgctgttgg taataatgat tccatatggg actttaaaca atggacgccc 720 catgtagttg taataaacct aatgcaaaac gatagctggc taatagatag agaaaagcgc 780 cttacgccaa ttcctgcaga tgcacaacgc atagcccatt atcaagcgtt tgtgcaaagc 840 attcgtgccg aataccccaa ggcgcaaata atatgcgcac tgggcagtat ggatgcaacc 900 gcaaacgaaa aatggccaaa ctacgtgcgc gaagctgtaa aaaatatgca agataatggc 960 gataataaaa tcgatactat tttctttgaa tacatcggct acggccaaca cccgcgcgta 1020 gcgcaacaca atgcgaatgc agataagtta actaaattta ttaagaaaaa aatgaaatgg 1080 tag 1083 <210> 67 <211> 973 <212> PRT <213> Microbulbifer degradans <400> 67 Met Asn Tyr Tyr Leu Asn Lys Lys Arg Leu Gly Gln Leu Leu Thr Gly 1 5 10 15 Ala Ala Ile Ile Pro Val Leu Tyr Ala Cys Gly Ser Gln Glu Lys Asn             20 25 30 Val Glu Pro Ala Thr Val Asn Trp His Lys Thr Ser Asp Gly Val Val         35 40 45 Val Ser Leu Gln Asp Ser Glu Ala Lys Lys Val Arg Leu Gln Val Ile     50 55 60 Asn Asp Arg Ile Val Arg Val Thr Ala Thr Pro Gln Gln Asp Phe Asn 65 70 75 80 Asn Leu Pro Asn Thr Leu Met Val Val Ala Lys Pro Glu Gln Thr Ala                 85 90 95 Phe Glu Val Lys Gln Asn Asp Ala Ser Val Val Leu Ser Thr Ala Asp             100 105 110 Leu Ser Ala Glu Val Ser Leu Val Thr Gly Val Val Ser Phe Lys Asp         115 120 125 Glu His Gly Lys Val Leu Thr Thr Glu Val Asp Arg Gly Asn Phe Gly     130 135 140 Ala Val Thr Arg Asp Pro Gly Val Val Asp Ala Asp Ser Phe Ala Ile 145 150 155 160 Arg Gln Gln Phe Thr Ser Asp Glu Asn Glu Gly Tyr Tyr Gly Leu Gly                 165 170 175 Gln Gln Gln Asp Gly Glu Val Asn Tyr Ala Gly Asp Asn Val Glu Leu             180 185 190 Thr Thr Tyr Asn Leu Glu Ile Ser Ile Pro Tyr Val Val Ser Ser Lys         195 200 205 Asp Tyr Ala Leu Leu Trp Asn Asn Thr Ser Ile Ser Arg Leu Gly Asp     210 215 220 Pro Asn Pro Pro Glu Pro Leu Lys Glu Gly Phe Lys Leu Phe Asp Ala 225 230 235 240 Asn Gly Asn Pro Gly Gly Leu Thr Ala Arg Tyr Phe Asp Gly Asp Lys                 245 250 255 Leu Leu Leu Glu Arg Val Glu Ala Asp Leu Asp Tyr Gln Phe Leu Ala             260 265 270 Gln Gly Ser Asn Arg Thr Thr Pro Met Pro Asp Glu Thr Ala Asp Ala         275 280 285 Lys Asn Leu Arg Ile Glu Trp Glu Gly Ser Ile Glu Ser Asp Thr Asn     290 295 300 Gly Val His Glu Leu Lys Met Tyr Ser Ser Gly Tyr Ala Lys Leu Tyr 305 310 315 320 Leu Asn Gly Glu Leu Val Leu Asp Arg Trp Arg Met Asn Trp Asn Pro                 325 330 335 Trp Tyr His Asn Thr Lys Leu Glu Met Gln Ala Gly Lys Lys Val Ala             340 345 350 Leu Lys Leu Asp Trp Gln Val Asp Gly Gly Tyr Met Arg Ile Lys Gln         355 360 365 His Lys Pro Leu Pro Val Ala Glu Gln Gly Arg Leu Ser Ile Ala Ser     370 375 380 Asp Thr Ala Lys Ala Ile Asp Tyr Tyr Phe Val Val Gly Asp Asn Lys 385 390 395 400 Asp Glu Leu Val Ser Gly Tyr Arg Thr Leu Thr Gly Lys Ala Val Met                 405 410 415 Leu Pro Lys Trp Val Phe Gly Phe Trp Gln Ser Arg Glu Arg Tyr Lys             420 425 430 Thr Gln Asp Glu Ile Ile Asp Ala Leu Gln Glu Tyr Arg Asp Arg Lys         435 440 445 Ile Pro Ile Asp Asn Ile Val Leu Asp Trp Ser Tyr Trp Pro Gln Asp     450 455 460 Ala Trp Gly Ser His Asp Phe Asp Glu Gln Phe Phe Pro Asp Pro Ser 465 470 475 480 Ala Leu Val Asp Lys Val His Glu Leu Asn Gly Asn Ile Met Ile Ser                 485 490 495 Val Trp Pro Lys Phe Tyr Pro Thr Thr Asp Asn Tyr Lys Ala Leu Asn             500 505 510 Ala Lys Gly Cys Met Phe Asn Lys Asn Ile Glu Gln Lys Asn Leu Asp         515 520 525 Trp Ile Gly Glu Gly Tyr Leu Asn Gly Phe Tyr Asp Ala Tyr Asn Pro     530 535 540 Glu Cys Arg Glu Met Phe Trp Ala Gln Ile Arg Asp Lys Ile Asn Val 545 550 555 560 His Gly Phe Asp Ala Trp Trp Leu Asp Ala Val Glu Pro Asp Ile His                 565 570 575 Ser Asn Leu Ser Phe Glu His Arg Lys Asp Leu Met Thr Pro Asn Ala             580 585 590 Leu Gly Thr Gly Ala Glu Val Phe Asn Ala Tyr Ala Leu Pro His Ala         595 600 605 Glu Thr Val Tyr Gln Gly Glu Arg Arg Asp Asp Gly Asp Lys Arg Ala     610 615 620 Phe Ile Leu Thr Arg Ser Gly Phe Ala Gly Ile Gln Arg Thr Gly Ser 625 630 635 640 Ala Ile Trp Ser Gly Asp Val Val Ser Arg Trp Ser Asp Leu Lys Glu                 645 650 655 Gln Ile Ala Ala Gly Val Gly Val Gly Ile Ser Gly Met Pro Tyr Trp             660 665 670 Thr Phe Asp Ile Gly Gly Phe Thr Pro Glu Asp Arg Tyr Arg Tyr Ser         675 680 685 Ala Lys Gly Ser Val Gly His Phe Ser Met Met Asn Glu Ser Glu Val     690 695 700 Pro Glu Trp Gln Glu Ile Asn Leu Arg Trp Phe Gln Phe Gly Thr Phe 705 710 715 720 Val Pro Leu Phe Arg Ser His Gly Gln Asn Pro Tyr Arg Glu Ile Tyr                 725 730 735 Asn Ile Ala Asp Lys Gly Thr Glu Val Tyr Asp Ser Met Val Trp Tyr             740 745 750 Thr Lys Thr Arg Tyr Arg Leu Met Pro Tyr Ile Tyr Ser Leu Val Gly         755 760 765 Asp Ala His His Lys Asp Gly Thr Phe Met Arg Ala Leu Val Met Asp     770 775 780 Phe Pro Ser Asp Leu Asn Val Arg Asp Ile Asn Asp Gln Tyr Met Phe 785 790 795 800 Gly Pro Ala Leu Leu Val Asn Pro Val Ser Glu Phe Lys Ala Arg Ser                 805 810 815 Arg Asp Val Tyr Leu Pro Ala Gly Ala Asp Trp Tyr Asp Phe Tyr Thr             820 825 830 Gly Val Lys His Thr Gly Gly Lys Thr Ile Lys Ala Asp Ala Pro Leu         835 840 845 Ala Lys Met Pro Ile Phe Val Lys Ala Gly Ser Ile Ile Pro Thr Gly     850 855 860 Val Glu Ile Gln His Val Tyr Asp Lys Pro Asp Ala Pro Tyr Thr Leu 865 870 875 880 Asn Val Tyr Thr Gly Ala Asn Gly Ser Phe Glu Ile Tyr Glu Asp Asp                 885 890 895 Gly Lys Thr Tyr Ala Tyr Glu Gln Gly Ala Trp Ala Arg Ile Pro Val             900 905 910 Ser Tyr Asn Asp Lys Thr Gly Glu Leu Thr Ile Gly Asp Arg Val Gly         915 920 925 Ser Phe Glu Gly Met Thr Lys Glu Arg Glu Phe Arg Val Arg Trp Ile     930 935 940 Ser Ala Lys Arg Asp Asp Ala Ala Asn Phe Asp Thr Gly Val Ala Lys 945 950 955 960 Ala Val Thr Tyr Thr Gly Lys Ala Ile Thr Ile Lys Arg                 965 970 <210> 68 <211> 2922 <212> DNA <213> Microbulbifer degradans <400> 68 gtgaattatt atttaaacaa aaagcgactg gggcaattgc tcaccggcgc ggccattatt 60 cccgtgctat atgcatgtgg ctcacaggaa aaaaacgtag agcctgcaac ggttaattgg 120 cataaaacaa gcgacggcgt cgttgtaagc ttgcaagata gcgaagcaaa aaaagtgcgc 180 ttgcaagtca ttaacgatcg gatagtacgt gttaccgcta cgccacagca ggatttcaac 240 aacctgccaa atacgcttat ggtggtggcc aagcccgagc aaacggcgtt tgaagttaaa 300 caaaacgatg catctgttgt gttatcaacg gcagatctat ctgccgaagt gtcattagta 360 actggtgttg taagttttaa agatgagcac ggcaaggtgc ttacaacaga agttgatcgc 420 ggcaattttg gggcggtaac ccgcgaccca ggtgtggtgg acgccgattc atttgctatt 480 cgccaacagt ttacaagcga cgaaaatgaa ggctactacg gtttaggtca gcagcaggat 540 ggcgaagtaa actacgctgg cgataacgta gagttaacaa cttacaactt agaaatttct 600 ataccttatg ttgtatcaag caaagattac gcgctgctat ggaacaatac ctcaatttct 660 cgtttgggcg accccaatcc acccgagcca ctaaaagagg gctttaaact ctttgacgct 720 aatggtaacc ccggcgggct aaccgcacgt tattttgatg gcgataaatt actgctcgag 780 cgtgtagagg ccgatttaga ttatcaattt ttagcgcaag gtagtaatcg cactacgccc 840 atgcctgatg aaaccgctga tgcaaaaaat ctgcgtattg aatgggaagg tagtatcgaa 900 tccgatacca acggtgtgca cgagttaaaa atgtattcca gtggctacgc taaattgtat 960 ttgaatggcg agttagtgtt agatcgctgg cgtatgaact ggaacccttg gtatcacaac 1020 accaagttag aaatgcaggc cggtaaaaaa gttgcattaa agttagattg gcaagtagat 1080 ggtggttata tgcgcataaa acagcataaa ccactgccgg tagcagagca gggacgtttg 1140 tctattgctt ccgataccgc gaaagccatt gattactact ttgtagttgg cgataacaag 1200 gatgagttgg tgtctggcta ccgtacgctc acaggtaaag cagtgatgct acctaagtgg 1260 gtgtttggtt tttggcaaag ccgcgagcgc tataaaacac aagatgaaat tatcgacgcc 1320 ttgcaagaat accgcgatcg taaaattcct atcgataaca ttgtattaga ttggagttat 1380 tggcctcagg atgcatgggg tagtcatgat ttcgacgagc aatttttccc cgacccatct 1440 gcactagtag ataaagtaca cgagctaaac ggcaatatta tgatttccgt atggcctaag 1500 ttttacccta caaccgacaa ctacaaagcg ctaaacgcta aaggttgtat gtttaataaa 1560 aacatcgagc agaaaaacct cgattggatt ggcgagggtt acctaaatgg cttttacgat 1620 gcctataacc cagagtgccg tgaaatgttt tgggcgcaaa ttcgcgataa gatcaatgtg 1680 cacggtttcg atgcttggtg gttagatgcg gtagagccag atatccattc caacctttct 1740 tttgagcacc gcaaagattt aatgacaccc aatgcactcg gcaccggtgc cgaagtgttt 1800 aacgcttacg ctttgccgca cgcagaaact gtttaccaag gcgagcgtag agatgacggt 1860 gacaagcgcg catttattct aacgcgttct gggtttgccg gtattcagcg caccggttcg 1920 gctatttgga gtggcgatgt ggtatcgcgc tggtccgact taaaagaaca aattgcagca 1980 ggtgtgggcg tgggcatttc tggtatgccg tattggacgt tcgatatcgg tggctttact 2040 ccagaagatc gctaccgtta tagcgccaaa ggttctgttg gtcatttctc tatgatgaac 2100 gaatcggaag tgcctgaatg gcaagaaatc aatctgcgtt ggttccaatt tggtaccttt 2160 gtgccgctgt ttaggtccca cggccaaaac ccatatcgcg aaatatataa catcgccgat 2220 aaaggcaccg aggtatacga cagcatggtg tggtacacca aaactcgcta tcgcttaatg 2280 ccttatattt attcgttagt tggcgatgct caccacaaag acggcacctt tatgcgcgct 2340 ctggtgatgg atttccctag cgaccttaat gtgcgcgata ttaacgacca gtatatgttt 2400 ggccccgcgc tactcgtaaa ccctgtgtcg gaatttaaag cgcgttcacg ggatgtgtat 2460 ctacctgcgg gcgcagattg gtacgatttc tatacaggtg tgaagcacac aggtggtaaa 2520 accattaagg ccgatgcacc gcttgccaaa atgcctattt ttgttaaggc cggctctatt 2580 attccaacag gtgtagaaat ccagcatgtg tacgataagc ccgatgctcc ttacaccctt 2640 aacgtgtata ccggtgcgaa tggcagcttc gaaatttatg aagatgacgg caaaacctac 2700 gcttacgagc aaggggcttg ggcgcgcatt cccgtttcgt acaacgataa aaccggtgag 2760 ctaaccattg gcgatcgcgt aggtagcttt gagggaatga ccaaagagcg cgaattccgc 2820 gtgcgctgga tatctgccaa gcgagacgat gccgccaatt tcgatacagg tgtggccaaa 2880 gccgttacct atacgggtaa ggcaataacc attaagcgct aa 2922 <210> 69 <211> 893 <212> PRT <213> Microbulbifer degradans <400> 69 Met Asn Lys His Phe Leu Val Gly Val Ile Thr Leu Gly Val Ile Leu 1 5 10 15 Gln Gly Leu Thr Ala Cys Ser Lys Ser Ala Ala Pro Asn Ala Asn Gln             20 25 30 Pro Gln Asp Thr Ala Ala Ser Thr Ala Thr Tyr Pro Phe Arg Asp Ala         35 40 45 Ser Leu Ser Val Asp Ala Arg Val Asp Asp Leu Val Ser Arg Leu Thr     50 55 60 Thr Thr Glu Lys Ile Ala Gln Met Phe Asn Asp Thr Pro Ala Ile Glu 65 70 75 80 Arg Leu Gly Ile Pro Ala Tyr Asn Trp Trp Asn Glu Ser Leu His Gly                 85 90 95 Val Ala Arg Ala Gly Lys Ala Thr Val Tyr Pro Gln Ala Ile Gly Leu             100 105 110 Ala Ser Thr Phe Asp Glu Asp Leu Met Leu Arg Val Ala Thr Ser Ile         115 120 125 Ser Asp Glu Gly Arg Ala Lys Tyr His Asp Phe Leu Ser Lys Asp Val     130 135 140 Arg Thr Ile Tyr Gly Gly Leu Thr Phe Trp Ser Pro Asn Ile Asn Ile 145 150 155 160 Phe Arg Asp Pro Arg Trp Gly Arg Gly Gln Glu Thr Tyr Gly Glu Asp                 165 170 175 Pro Phe Leu Thr Gly Arg Met Ala Ile Asn Phe Val Lys Gly Ile Gln             180 185 190 Gly Glu Asn Asp Asn Ser Asp Tyr Leu Lys Ala Val Ala Thr Ile Lys         195 200 205 His Tyr Ala Val His Ser Gly Pro Glu Lys Thr Arg His Ser Asp Asp     210 215 220 Tyr His Pro Thr Arg Lys Asp Leu Phe Glu Thr Tyr Leu Pro Ala Phe 225 230 235 240 Arg Met Ala Ile Ala Glu Thr Asn Val Gln Ser Leu Met Cys Ala Tyr                 245 250 255 Asn Arg Val Asp Gly Ala Pro Ala Cys Gly Asn Asn Glu Leu Met Gln             260 265 270 Glu Ile Leu Arg Gly Asp Met Gly Phe Asn Gly Tyr Val Val Ser Asp         275 280 285 Cys Gly Ala Ile Ala Asp Phe Tyr Glu Ser Arg Ser His His Val Val     290 295 300 Asp Ser Pro Ala Glu Ala Ala Ala Trp Ala Val Lys Ser Gly Thr Asp 305 310 315 320 Leu Asn Cys Gly Asp Ser His Gly Asn Thr Tyr Thr Asn Leu His Tyr                 325 330 335 Ala Leu Gln Gln Gly Leu Ile Thr Glu Asp Tyr Ile Asp Ile Ala Val             340 345 350 Lys Arg Leu Phe Lys Ala Arg Ile Lys Leu Gly Met Phe Asp Glu Gln         355 360 365 Asp Arg Val Pro Tyr Ser Glu Ile Gly Met Asp Val Val Gly Ser Pro     370 375 380 Lys His Leu Ala Leu Thr Gln Glu Ala Ala Glu Lys Ser Ile Val Leu 385 390 395 400 Leu Lys Asn Asn Gly Val Leu Pro Leu Lys Ala Gly Val Lys Val Ala                 405 410 415 Val Ile Gly Pro Asn Ala Val Asp Glu Asp Val Leu Val Gly Asn Tyr             420 425 430 His Gly Val Pro Val Lys Pro Val Leu Pro Leu Glu Gly Ile Val Asn         435 440 445 Arg Val Gly Glu Ala Asn Val Phe Tyr Ala Pro Gly Ser Ala Gln Ile     450 455 460 Ala Asp Ile Tyr Ser His Tyr Glu Pro Ile Ser Ala Glu Asn Phe Tyr 465 470 475 480 His Lys Asp Ala Asn Gly Asn Leu Ala Ala Gly Leu Lys Ala Glu Tyr                 485 490 495 Tyr Ala Asp Tyr Tyr Asn Ala Ala Glu Ile Asn Asp Asp Thr Phe Ser             500 505 510 Ala Thr Pro Ala Leu Asn Arg Ile Asp Ala Asp Ile Asn Phe Ser Trp         515 520 525 Pro Val Ser Pro Ile Asp Asn Ser Leu Asp Asp Glu Phe Ser Ala Val     530 535 540 Trp Thr Gly Ile Leu Lys Pro Lys Lys Ser Gly Ser Tyr Arg Phe Ser 545 550 555 560 Gly Thr Val Ala Leu Ala Ile Asn Gly Lys Pro Val Asn Gly Ala Val                 565 570 575 Asn Leu Lys Ala Gly Glu Ser Tyr Asn Ile Lys Ala Ile Phe Gly Val             580 585 590 Gln Lys Trp Trp Pro Val Asn Ala Ile His Pro Tyr Gly Lys Leu Thr         595 600 605 Trp Leu Asp Glu Ser Arg Asp Leu Glu Glu Glu Ala Leu Ala Ala Ala     610 615 620 Arg Lys Ala Asp Val Ile Ile Phe Met Gly Gly Ile Asp Ala His Leu 625 630 635 640 Glu Gly Glu Glu Met Pro Leu Glu Leu Asp Gly Phe Thr His Gly Asp                 645 650 655 Arg Thr His Ile Asn Leu Pro Lys Val Gln Thr Asn Leu Leu Lys Gln             660 665 670 Leu Lys Ala Thr Gly Lys Pro Val Val Met Val Asn Phe Ser Gly Ser         675 680 685 Ala Met Ala Leu Asn Trp Glu Ser Glu Lys Leu Asp Ala Ile Leu Gln     690 695 700 Ala Phe Tyr Pro Gly Glu Ala Thr Gly Thr Ala Leu Ala Asn Ile Leu 705 710 715 720 Trp Gly Asp Val Ser Pro Ser Gly Arg Leu Pro Val Thr Phe Tyr Lys                 725 730 735 Gly Val Asp Asp Leu Pro Ala Phe Asn Asp Tyr His Met Glu Asn Arg             740 745 750 Thr Tyr Lys Phe Tyr Arg Gly Glu Pro Leu Tyr Ala Phe Gly His Gly         755 760 765 Leu Gly Tyr Val Asp Phe Ala Tyr Asn Asn Leu Val Val Ala Asn Thr     770 775 780 Ala Glu Ala Gly Lys Ala Leu Pro Ile Ala Val Ser Val Thr Asn Thr 785 790 795 800 Gly Lys Met Gln Ala Glu Asp Val Ala Gln Val Tyr Ile Ser Leu Leu                 805 810 815 Asp Ala Pro Ala Asn Thr Pro Ile Arg Asp Leu Lys Ala Phe Lys Arg             820 825 830 Thr Lys Leu Ala Ala Gly Glu Ser Thr Glu Leu Glu Phe Asn Leu Pro         835 840 845 Ala Arg Val Leu Thr Tyr Ile Asp Asp Asn Gly Lys Thr Gln Thr Tyr     850 855 860 Thr Gly Arg Val Glu Val Thr Val Gly Ser Gly Gln Lys Gly Tyr Val 865 870 875 880 Lys Glu Asn Ala Ile Ala Val Ala Thr Ile Asn Val Gln                 885 890 <210> 70 <211> 2682 <212> DNA <213> Microbulbifer degradans <400> 70 atgaataaac actttttagt aggtgtaatt acgttagggg taattctgca ggggctaact 60 gcatgtagca aaagcgctgc acctaatgcc aatcaaccgc aagataccgc agctagtacg 120 gctacctacc cgtttaggga tgcaagctta agtgtagatg cccgcgtaga cgacttggta 180 tcgcgtttaa ccacaaccga aaaaattgcc caaatgttta acgatacgcc cgcaatcgag 240 cgattgggta ttcccgccta caattggtgg aacgaatcgt tgcacggtgt ggcccgtgcg 300 ggtaaagcaa cggtataccc gcaggcaata ggcttagcgt ctacatttga tgaagactta 360 atgttgcgcg tggctacttc tatttctgat gaggggcgcg ctaagtatca cgacttccta 420 tcgaaagacg tgcgcaccat atacggcggg cttacctttt ggtcgccaaa tattaatatc 480 ttccgcgacc cgcgttgggg cagggggcaa gaaacctacg gtgaagaccc gttcttaacg 540 gggcgtatgg ccattaattt tgttaagggt attcaaggcg aaaacgacaa cagcgattac 600 ctaaaagccg tagcgacaat taagcactat gccgtacaca gcggccccga aaaaacgcgt 660 cattcggatg actaccatcc aacccgtaaa gatttattcg aaacctattt gcctgcattt 720 cgcatggcaa tagcagagac taacgtgcaa tcgttaatgt gtgcctacaa ccgtgtagat 780 ggggcacctg cctgtggcaa taatgaatta atgcaagaaa ttttgcgtgg cgatatgggc 840 tttaacggtt atgtcgtgtc tgactgtggc gccattgccg atttttacga gagtagatcg 900 caccacgtgg ttgactcacc tgcagaggct gcagcgtggg ccgttaaatc gggtaccgat 960 ttaaactgtg gcgattcaca tggcaatacc tacaccaacc tgcattacgc gttacagcaa 1020 ggtttaatta cagaagatta tattgatata gcggtaaagc gtttgtttaa agcgcgtatt 1080 aagcttggca tgtttgacga gcaagaccgc gtgccttaca gcgaaattgg tatggatgtt 1140 gtaggttcac ctaagcacct agcgctaacc caagaagcgg cagaaaaatc tattgtgctg 1200 ctaaaaaaca atggtgtatt gccattaaaa gcaggggtaa aggtagccgt aatagggcca 1260 aatgcagttg atgaagatgt attggtaggc aactaccacg gcgtaccagt gaaacctgtg 1320 ttgccgctag aggggattgt taatcgtgtt ggcgaggcca acgtatttta tgccccaggc 1380 agtgcacaaa tagccgatat atacagccac tacgaaccga taagtgcaga aaatttttat 1440 cataaagatg caaatggtaa tttagctgca ggcttaaaag cagagtatta cgccgattat 1500 tacaacgcag ctgaaattaa cgacgatacc tttagcgcaa ccccagcgtt aaatagaatt 1560 gatgcagata ttaatttctc ttggcctgta tcgcctattg ataattcgtt agatgatgaa 1620 tttagtgcag tatggacagg catacttaaa ccgaaaaagt cgggtagcta ccgtttctcg 1680 ggcacggttg cattagccat taacggcaaa cctgttaatg gggctgttaa cctaaaggca 1740 ggtgaaagct ataacataaa agctattttt ggcgtgcaaa aatggtggcc cgttaatgca 1800 atacacccgt acggaaaact tacttggcta gatgagtcgc gcgatttaga agaagaggca 1860 ttagctgctg cccgaaaagc cgatgtgatt atttttatgg gcggtataga tgcgcacctt 1920 gaaggcgaag aaatgccgct agagctagat ggctttactc acggtgatcg tacgcacatt 1980 aatttaccta aagtacaaac caatttgctt aaacaattaa aagcaacggg taaacctgtt 2040 gtaatggtta actttagtgg tagtgccatg gctttaaatt gggaaagcga aaagctagac 2100 gcaatactgc aagcgtttta cccaggtgaa gcaaccggta cagcgttagc taatattttg 2160 tggggcgatg taagcccgag tggccgctta cctgtaacct tttacaaagg cgtagacgat 2220 ctaccagcat ttaatgatta ccacatggaa aaccgcacct ataaatttta ccgcggtgag 2280 cctttgtatg catttggcca cggtttaggt tacgttgatt ttgcttataa caatttagtc 2340 gtagcaaata ctgcagaagc gggcaaagcg ctacctatag ctgtaagcgt aaccaatacc 2400 ggtaaaatgc aagcagaaga cgttgcccaa gtttatataa gtttgctaga tgcccccgca 2460 aacacgccca tccgcgattt aaaagcgttt aaacgtacca agcttgcggc aggcgaaagc 2520 accgagcttg aatttaactt gccggcgaga gtgcttacct atatagacga taatggtaaa 2580 acccaaacct atactggcag ggtagaagtt actgttggct ctgggcaaaa gggatacgta 2640 aaagaaaatg cgatagctgt agcgactatt aacgttcagt ag 2682 <210> 71 <211> 317 <212> PRT <213> Microbulbifer degradans <400> 71 Met Tyr Thr Tyr Val Ser Ala Ile Ala Leu Phe Ile Phe Ser Ile Ala 1 5 10 15 Ser Ser Cys Cys Val Ala Gln Asn Pro Leu Asp Phe Gly Ser Asn Ile             20 25 30 Lys Thr Ala Asp Pro Ser Gly His Ile Trp Ala Asp Gly Arg Met Tyr         35 40 45 Leu Tyr Thr Ser His Asp Gln Glu Cys Gln Glu Asp Phe Tyr Met Lys     50 55 60 Asp Trp His Thr Phe Ser Ser Ser Asp Leu Ile Asn Trp Thr Ala His 65 70 75 80 Gly Pro Ser Leu Ser Val Ala Asp Ile Thr Trp Ala Asp Asn Tyr Ala                 85 90 95 Trp Ala Pro Asp Ala Ala Tyr Lys Asn Gly Lys Tyr Tyr Leu Phe Phe             100 105 110 Pro Ala Gly Thr Gly Val Lys Asp Arg Val Asn Pro Glu Lys Ser Thr         115 120 125 Lys Trp Met Gly Ile Gly Val Ala Val Ser Asp Ser Pro Thr Gly Pro     130 135 140 Phe Lys Asp Ala Ile Gly Ala Pro Leu Trp Thr Asp Pro Tyr Ala Asn 145 150 155 160 Asp Pro Ser Ile Phe Ile Asp Asp Asp Gly Lys Gly Tyr Leu Tyr Phe                 165 170 175 His Gly Lys Gly Ala Asp Tyr Leu Val Ala Glu Met Ala Asp Asp Leu             180 185 190 Leu Ser Val Lys Gly Glu Phe His Lys Met Asp Met Gly Gly Tyr Glu         195 200 205 Pro Lys Met Glu Gly Pro Trp Val Phe Lys Arg Glu Gly Met Tyr Tyr     210 215 220 Phe Thr Met Pro Glu Asn Asn Arg Ser Leu Ala Tyr Tyr Met Ala Lys 225 230 235 240 Ser Pro Phe Gly Pro Trp Glu Tyr Lys Gly Ile Phe Met Gln Glu Glu                 245 250 255 Gly Gly Asn Asn His His Ser Ile Val Gln Phe Lys Gly Lys Trp Ile             260 265 270 Leu Phe Tyr His Arg Trp Leu Met Gly Glu Gly Glu Cys Lys Lys Lys         275 280 285 Gln Arg His Thr Ala Ala Glu Tyr Leu His Phe Asn Ala Asp Gly Thr     290 295 300 Ile Lys Glu Val Lys Arg Thr Arg Glu Gly Leu Thr Lys 305 310 315 <210> 72 <211> 954 <212> DNA <213> Microbulbifer degradans <400> 72 atgtacacat atgtatccgc catagcacta tttatatttt caattgcctc gtcgtgttgt 60 gttgcccaaa acccgctcga ctttggcagt aatattaaaa ccgcagatcc gtctggccat 120 atatgggctg atggcagaat gtacctttac acctcgcacg accaagaatg ccaagaagat 180 ttttatatga aggattggca taccttttcg tccagcgact taataaattg gactgcccac 240 ggcccaagtt tatctgtagc ggatattacg tgggcagata actacgcatg ggcgcccgac 300 gcggcctata aaaatgggaa gtactatttg ttctttccgg cgggaaccgg tgttaaagat 360 agagtaaacc ccgaaaaaag cactaagtgg atgggcattg gtgttgcagt aagcgatagc 420 cctacaggcc cctttaaaga tgcgattggc gcccccttgt ggaccgaccc ctatgccaac 480 gacccaagta tttttataga tgatgacggc aagggctact tatattttca cggtaaaggt 540 gcagactacc tagtagccga aatggcagac gatttactga gtgtaaaagg tgagtttcac 600 aaaatggata tgggcggtta cgagccaaaa atggagggcc cttgggtttt taagcgcgag 660 ggaatgtatt actttaccat gccagaaaac aatcgttcac ttgcttacta tatggcgaaa 720 tctccctttg ggccgtggga atacaagggc atttttatgc aagaagaagg cggtaacaac 780 caccattcta ttgtgcaatt taaaggcaag tggatattgt tttatcaccg ctggttaatg 840 ggcgaaggcg agtgtaaaaa gaagcaacgc cacaccgcag cggaatacct tcactttaat 900 gccgacggca caattaaaga agtaaaaaga acgcgcgagg ggttaactaa gtag 954 <210> 73 <211> 577 <212> PRT <213> Microbulbifer degradans <400> 73 Met Lys Ile Lys Cys Leu Leu Leu Ala Val Tyr Ala Gly Leu Leu Ala 1 5 10 15 Ala Cys Ala Leu Asp Ala Pro Leu Lys Thr Ser Ser Lys Pro Leu Ala             20 25 30 His Phe Ser Trp Phe Glu Tyr Gln Gly Asn Asp Glu Ile Phe Lys Ala         35 40 45 Pro Leu Ala Ser Asn Gln Tyr Gln Asn Pro Ile Leu Ala Gly Tyr His     50 55 60 Pro Asp Pro Ser Ile Val Arg Val Gly Glu Asp Tyr Tyr Leu Val Asn 65 70 75 80 Ser Thr Phe Gly Phe Tyr Pro Gly Ile Pro Val Phe His Ser Arg Asp                 85 90 95 Leu Val Asn Trp Thr Gln Leu Gly Asn Ala Ile His Arg Pro Glu Gln             100 105 110 Leu Ser Phe Asp Gly Ile His Leu Gly Tyr Asn Gly Val Tyr Ala Pro         115 120 125 Ala Ile Glu Tyr Arg Asp Gly Thr Phe Tyr Val Ile Asn Thr Cys Val     130 135 140 Ala Cys Gly Gly Asn Phe Ile Val Thr Ala Thr Asn Pro Ala Gly Pro 145 150 155 160 Trp Ser Asp Pro Ile Trp Leu Pro Glu Val Ile Gly Ile Asp Pro Ser                 165 170 175 Leu Phe Phe Asp Glu Asp Gly Lys Thr Tyr Ile Val His His Arg Asn             180 185 190 Pro Pro Val Gln Lys Tyr Pro Ala His Thr Ala Leu Trp Val Met Glu         195 200 205 Val Asp Ser Lys Thr Phe Ala Pro Val Ser Asp Asp Val Met Leu Val     210 215 220 Asp Gly Gly Asp Glu Ala Pro Trp His Thr Glu Tyr Ile Glu Gly Pro 225 230 235 240 His Ile Tyr Lys Ile Asp Gly Thr Tyr Tyr Leu Tyr Ala Pro Gly Gly                 245 250 255 Gly Thr Gly Tyr Phe His Gly Gln Leu Val Tyr Arg Ser Asp Asn Val             260 265 270 Phe Gly Pro Tyr Glu Ala Asn Pro Asn Asn Pro Val Leu Thr Gln Val         275 280 285 Gly Leu Pro Asp Asp Arg Glu His Pro Val Thr Ala Thr Gly His Ala     290 295 300 Asp Leu Phe Gln Asp Thr Asn Gly Asp Trp Trp Thr Val Phe Leu Gly 305 310 315 320 Thr Arg Val Tyr Asp Leu Ala Lys Pro Pro Gln Asp Pro Gly Asn Phe                 325 330 335 Ala Thr Gly Arg Glu Thr Phe Met Leu Pro Val Thr Trp Gln Asn Gly             340 345 350 Trp Pro His Val Leu Glu Lys Gly Glu Ala Val Pro Tyr Arg Val Thr         355 360 365 Lys Pro Lys Leu Pro Ala Gly Lys Pro Ala Pro Arg Ala Met Thr Gly     370 375 380 Asn Phe Thr Val Arg Glu Glu Phe Thr Asn Ala Ser Leu Ala Pro His 385 390 395 400 Trp Leu Phe Val Arg Thr Pro Arg Ser Lys Trp Trp Gln Thr Gly Asn                 405 410 415 Gly Glu Leu Ile Leu Glu Ala Arg Ala Asp Thr Ile Gly Ala Val Asn             420 425 430 Gln Pro Ser Phe Ile Gly Arg Arg Leu Ala His Met Thr Ala Ser Phe         435 440 445 Ala Thr Gln Leu Thr Phe Asn Pro His Thr Val Gly Asp Glu Ala Gly     450 455 460 Leu Leu Ala Val Gln Asn Asp Glu His Phe Tyr Ala Phe Gly Leu Gly 465 470 475 480 Leu Asn Ser Lys Gly Gln Thr Val Leu Arg Val Arg Lys Lys Ala Gly                 485 490 495 Lys Asn Glu Ser Ile Arg Gly Asp Thr Val Ala Glu Gln Val Val Lys             500 505 510 Leu Lys His Gly His Pro Ile Tyr Leu Arg Val Asn Ile Gly Lys Ala         515 520 525 Glu Leu Asn Phe Ala Tyr Ser Thr Asn Gly Lys Arg Tyr Thr Thr Leu     530 535 540 Leu Asn Gln Ala Asp Ala Asn Leu Leu Thr Thr Ala Lys Ala Gly Gly 545 550 555 560 Phe Thr Gly Ala Val Val Gly Met Tyr Ala Glu Ser Thr Ala Gln Gln                 565 570 575 Asn      <210> 74 <211> 1734 <212> DNA <213> Microbulbifer degradans <400> 74 atgaaaatta agtgcttact ccttgctgtt tacgcgggtc tacttgcggc ttgcgcgctg 60 gacgcgccgc tcaaaacctc aagtaaaccg ctagcgcatt tttcgtggtt tgaatatcaa 120 ggtaacgacg agatatttaa ggctccactc gcctcaaatc aataccaaaa ccccatactc 180 gccggctacc acccagaccc aagtattgtg cgagtaggcg aagattatta tttggtgaac 240 tccacctttg gcttctaccc tggcattcca gtatttcaca gccgtgactt agtgaattgg 300 acccaactgg gtaacgctat tcaccgccca gagcaacttt catttgatgg tattcactta 360 ggctacaacg gcgtttatgc accggcaatc gaataccgcg acgggacctt ttacgtaata 420 aatacctgcg tagcctgcgg aggaaatttt atcgttaccg ccaccaatcc cgcgggcccc 480 tggtcagacc caatatggct accagaggta attggcatag acccctcgct atttttcgac 540 gaggacggca aaacctatat cgtgcatcat cgtaatccac ctgtgcagaa ataccctgcc 600 cacacagccc tgtgggtaat ggaagttgac tccaaaacat ttgcgccggt atctgacgat 660 gtaatgcttg tggacggtgg cgacgaagcg ccatggcaca cagaatatat tgaagggccg 720 catatatata aaattgatgg cacctactac ctctatgccc ctggtggcgg cacgggatac 780 ttccacggcc aattggtgta tagatctgac aatgtatttg gaccctacga agccaacccc 840 aataaccctg tgttgactca agttggttta cccgacgaca gagaacaccc tgtaacggca 900 acgggtcatg cagatttatt tcaagatacc aacggcgact ggtggacggt atttctgggt 960 actcgcgttt acgatttagc taagccacca caagaccccg gcaattttgc caccggacgc 1020 gaaacattta tgttgccagt aacatggcaa aacggctggc cacacgtgct cgaaaaaggc 1080 gaggctgtgc cctaccgagt aaccaaaccc aaattacctg caggcaaacc cgccccgcgc 1140 gcaatgactg gaaactttac tgtgcgcgag gaatttacca acgcttcgct tgccccccac 1200 tggctatttg ttcgcacacc gcgttccaaa tggtggcaaa caggtaatgg cgaacttatt 1260 ttagaagcgc gcgccgatac cattggggca gttaaccagc cgtcgtttat tggccgacgg 1320 ctcgctcata tgacggcctc cttcgccacc caactaacct ttaacccaca caccgttggc 1380 gacgaagcag ggttactcgc cgtacaaaac gacgaacact tttacgcctt tggcctaggg 1440 ttaaacagta aagggcaaac cgttttgcgc gtgcgtaaaa aagcgggtaa aaatgaatcg 1500 ataaggggag atacggttgc cgagcaggtt gttaagctta agcacggcca ccctatttac 1560 ctgcgtgtaa atataggtaa agccgaatta aatttcgcgt atagcaccaa cggcaaacgc 1620 tacaccacct tgttgaacca agccgatgcc aacctactta ccacagctaa agcgggcggg 1680 tttactggcg cagtagtggg tatgtacgcc gaatccaccg cacaacaaaa ctaa 1734 <210> 75 <211> 566 <212> PRT <213> Microbulbifer degradans <400> 75 Met Arg Leu Leu Pro Ile Leu Leu Val Ser Leu Leu Pro Leu Leu Ser 1 5 10 15 Ser Cys Thr Ser Ala Ile Asn Gly Gln Gln Asn Ser Gln Thr Ser Pro             20 25 30 Val Phe Asp Trp Phe Glu Tyr Ala Gly Ser Asp Ala Leu Tyr Asn Thr         35 40 45 Val Ala Pro Ser Lys Asn Ala Tyr Thr Asn Pro Val Ile Lys Gly Phe     50 55 60 Tyr Pro Asp Pro Ser Ile Val Arg Val Gly Ala Asp Tyr Tyr Leu Val 65 70 75 80 Asn Ser Ser Phe Gly Tyr Phe Pro Gly Val Pro Ile Phe His Ser Thr                 85 90 95 Asp Leu Val Asn Trp Val Gln Ile Gly Asn Ile Leu Glu Arg Pro Ser             100 105 110 Gln Leu Gln Ile Pro Ser Gly Met Gly Val Ser Arg Gly Ile Phe Ala         115 120 125 Pro Thr Leu Arg His His Asn Gly Ile Phe Tyr Met Ile Thr Thr Met     130 135 140 Val Asp Gly Gly Gly Asn Phe Ile Val Thr Ala Lys Asn Pro Ala Gly 145 150 155 160 Pro Trp Ser Asp Pro Val Trp Leu Pro Glu Val Gly Gly Ile Asp Pro                 165 170 175 Asp Leu Phe Phe Asp Asp Asn Gly Lys Ala Tyr Ile Leu Asn Asn Asp             180 185 190 Ala Pro Ile Gly Glu Pro Leu Tyr Asp Gly His Arg Ala Ile Trp Ile         195 200 205 Arg Glu Phe Asp Leu Ala Thr Leu Lys Thr Val Gly Asp Ala Lys Leu     210 215 220 Ile Val Asn Gly Gly Val Asp Ile Thr Thr Lys Pro Val Trp Ile Glu 225 230 235 240 Gly Pro His Leu Phe Lys Asn Lys Gly Ala Tyr Tyr Leu Ile Asn Ala                 245 250 255 Glu Gly Gly Thr Ser Val Asn His Ser Gln Val Val Phe Lys Ala Gln             260 265 270 Ser Pro Trp Gly Pro Tyr Ile Pro Trp Glu Asn Asn Pro Ile Leu Thr         275 280 285 Gln Arg His Leu Pro Ala Asp Arg Ala Asn Pro Val Thr Ser Val Gly     290 295 300 His Val Asp Leu Val Gln Thr Gln His Gly Asp Trp Trp Ala Val Phe 305 310 315 320 Leu Gly Cys Arg Pro Tyr Lys Asp Asn Tyr Tyr Asn Thr Gly Arg Glu                 325 330 335 Thr Phe Leu Leu Pro Val Asp Trp Ser Gly Glu Tyr Pro Val Ile Leu             340 345 350 Arg Gly Asp Ala Glu Val Pro Tyr His His Gln Arg Pro Gln Leu Gly         355 360 365 Ala Ser Gln Gln Pro Ala Ile Ala Leu Ser Gly Asn Phe Ile Glu Arg     370 375 380 Asp Glu Phe Asp Ser Ala Leu Lys Leu Tyr Trp Arg Lys Val Arg Thr 385 390 395 400 Pro Thr Asn Asn Phe Thr Asp Leu Thr Ser Gln Lys Gly Lys Leu Val                 405 410 415 Leu Thr Ala Asn Asn Thr Asp Leu Ser Asp Phe Gly Ser Pro Ala Phe             420 425 430 Ile Ala Arg Ala Gln Gln His Leu Thr Gly Ser Ala Thr Thr Lys Leu         435 440 445 Val Tyr Thr Pro Pro His Val Gly Asp Lys Ala Gly Ile Ala Ala Phe     450 455 460 Gln Asn Asp Glu Tyr Phe Tyr Ala Leu Thr Val Thr Lys Asn Asn Ser 465 470 475 480 Gly Leu Ala Ile Gln Leu Glu Lys Gln Leu Gly Lys Asn Lys Glu Ile                 485 490 495 Val Ala Gln Tyr Pro Leu Gln Glu Lys Thr Leu Arg Asn Gly Leu Tyr             500 505 510 Leu Lys Ile Glu Phe Asn Asn Asp Lys Tyr Asp Phe Ser Tyr Ser Thr         515 520 525 Asn Asn Thr Lys Trp Gln Ser Val Gly Glu Thr Gln Asp Gly Thr Ile     530 535 540 Leu Ser Thr Gln Ser Ala Gly Gly Phe Val Gly Ala Thr Leu Gly Ile 545 550 555 560 Phe Ala Tyr Thr Ala His                 565 <210> 76 <211> 1701 <212> DNA <213> Microbulbifer degradans <400> 76 atgcgacttt tacctatctt actcgttagc ttacttccac tgctctcaag ctgcacaagc 60 gccataaacg ggcaacaaaa tagccaaacc tcgcctgtat ttgattggtt tgaatacgcg 120 ggaagcgatg ctttatacaa cacggttgcg ccaagtaaaa atgcctatac caacccagta 180 ataaaagggt tttatccaga tccaagcatt gtaagagtgg gagcagatta ctacctcgtg 240 aactcttcat ttggctactt ccctggcgtg ccgatatttc atagcacaga tttagtgaat 300 tgggttcaaa taggtaatat tctcgagcgc ccatcacaat tacaaatacc cagcggcatg 360 ggtgtgtcgc gaggtatatt cgccccaaca ctgcgccacc acaacggtat tttttacatg 420 attactacaa tggtagacgg tggcggcaat tttattgtta ctgcaaaaaa ccccgcaggc 480 ccttggtcgg acccagtatg gttacctgaa gtgggcggta tagacccaga tttatttttt 540 gatgacaacg gcaaagccta catacttaac aacgacgccc ccattggcga gccgctttac 600 gatggccacc gagccatttg gattcgcgaa ttcgacttag ccacattaaa aaccgttggc 660 gacgccaagt taatagtaaa cggcggtgta gatataacta ccaaacccgt ttggatagaa 720 ggcccacacc ttttcaaaaa taaaggcgct tactatttaa ttaatgcaga aggtggcacc 780 agcgtgaatc acagccaagt tgtatttaaa gcgcaaagcc cttgggggcc gtatattcct 840 tgggaaaaca atccaatttt aacacagcgc catttaccgg ctgatcgcgc caaccccgtc 900 acatccgttg gccatgtcga tttagtacaa actcaacatg gcgactggtg ggcggtattt 960 ttaggctgca ggccctataa agataactac tacaataccg gccgcgaaac atttttatta 1020 ccggtagatt ggtctggcga ataccccgtc attcttcgcg gcgatgccga ggtgccctat 1080 catcaccaac gcccccaatt gggagcatcc caacaaccag ccattgccct tagcggtaac 1140 tttattgagc gcgatgaatt tgactcagca cttaaacttt attggcgcaa ggttcgcacc 1200 cccacaaaca actttacaga tttaacctct caaaaaggca agcttgtttt aactgcaaac 1260 aatacagatt taagcgactt tggatcacca gcatttattg cgcgcgcaca gcagcaccta 1320 acaggcagcg caacaaccaa actggtttac acacccccac acgtgggcga caaagcgggt 1380 attgctgcct ttcaaaacga tgagtatttt tatgcgctta ccgttacaaa aaataatagc 1440 ggccttgcca tacaactaga aaaacaactt ggcaagaaca aagaaattgt tgcgcaatat 1500 ccactacaag aaaaaacgct tcgcaatggc ttatatttga aaatagaatt taataacgac 1560 aaatatgatt tcagctattc cacaaataac accaagtggc aatcggtagg cgaaacacaa 1620 gatggaacta tattaagcac gcaaagtgca ggcgggtttg taggtgccac gctaggtata 1680 tttgcatata ccgcgcacta a 1701 <210> 77 <211> 319 <212> PRT <213> Microbulbifer degradans <400> 77 Met Ser Met Phe Asn Lys Lys Thr Leu Ala Ala Gly Ile Val Ala Ala 1 5 10 15 Cys Leu Thr Asn Val Ser Ala Ser Tyr Ala Ala Asn Pro Ala Ile Thr             20 25 30 Asp Thr His Thr Ala Asp Pro Ala Ala Leu Val His Gly Asp Thr Val         35 40 45 Tyr Leu Tyr Val Gly Asn Asp Glu Ala Lys Asp Asn Arg Val Phe Tyr     50 55 60 Asp Leu Lys Lys Trp Leu Val Tyr Ser Ser Lys Asp Met Val Asn Trp 65 70 75 80 Thr Asn His Gly Ser Pro Leu Ala Ala Thr Asp Phe Lys Trp Ala Ser                 85 90 95 Gly Asp Ala Trp Ala Ala His Thr Val Glu Lys Asp Gly Lys Phe Tyr             100 105 110 Trp Tyr Thr Thr Val Arg His Ala Thr Ile Asn Gly Phe Ala Ile Gly         115 120 125 Val Ala Val Ser Asp Ser Pro Thr Gly Pro Phe Lys Asp Ala Leu Gly     130 135 140 Lys Ala Leu Ile Ser Asn Asp Met Thr Thr Asp Thr Asp Ile Asp Trp 145 150 155 160 Asp Asp Ile Asp Pro Ala Val Phe Ile Asp Asp Asp Gly Gln Ala Tyr                 165 170 175 Ile Phe Trp Gly Asn Thr Lys Pro Arg Trp Ala Lys Leu Lys Pro Asn             180 185 190 Met Ile Glu Leu Asp Gly Pro Ile His Ala Ile Asp Ile Pro His Phe         195 200 205 Thr Glu Ala Leu Tyr Val His Lys His Gly Glu Tyr Tyr Tyr Leu Ser     210 215 220 Tyr Ala Thr Gly Phe Pro Glu Lys Thr Ala Tyr Ala Met Ser Lys Ser 225 230 235 240 Ile Glu Gly Pro Trp Glu Tyr Lys Gly Ile Leu Asn Glu Leu Ala Gly                 245 250 255 Asn Ser Asn Thr Asn His Gln Ser Val Ile Asp Phe Lys Gly Lys Ser             260 265 270 Tyr Phe Ile Tyr His Asn Gly Gly Leu Gly Gln Asp Gly Gly Ser Phe         275 280 285 Arg Arg Ser Val Cys Ile Asp Tyr Leu Asn Tyr Asn Ala Asp Gly Thr     290 295 300 Ile Lys Arg Ile Val Met Thr Ser Glu Gly Val Asp Pro Val Lys 305 310 315 <210> 78 <211> 960 <212> DNA <213> Microbulbifer degradans <400> 78 gtgagtatgt ttaataaaaa aacactagca gccggtattg tagctgcatg tttaactaac 60 gtaagtgcaa gctatgctgc caaccccgca attaccgata ctcacacggc cgatcccgct 120 gcgttagtgc acggcgatac cgtttatttg tacgtgggta acgatgaagc gaaggataac 180 cgcgtatttt acgatcttaa aaaatggttg gtgtattcat caaaagatat ggtgaactgg 240 accaatcacg gttcgccgtt agctgcaacg gattttaagt gggccagcgg cgatgcgtgg 300 gcggcgcaca cggtagaaaa agatggcaag ttttattggt ataccacggt gcgtcacgca 360 accattaatg gttttgccat tggcgttgca gtaagtgata gccctacagg gccattcaaa 420 gatgctttgg gtaaagcact aataagtaat gacatgacca ccgataccga tattgattgg 480 gacgatatag acccagcagt atttattgac gacgatggcc aagcgtatat tttttggggc 540 aacaccaaac cgcgctgggc caagttaaaa cccaatatga ttgaactaga tggacctatt 600 cacgcaatcg atattccaca ctttaccgaa gcgctatacg tgcacaaaca cggtgaatat 660 tactacttaa gctatgcgac aggctttcca gaaaaaacag cttacgctat gagcaaatct 720 atagaagggc cgtgggaata caaaggcatt cttaatgaat tggctggtaa ctcaaatact 780 aatcaccaat ctgtcatcga ttttaagggc aagtcatact ttatttatca caatggtggc 840 ttgggtcaag atggcggtag cttccgtcgc agtgtatgta tcgattattt gaactacaac 900 gcggatggta ctatcaagcg aattgtaatg acatcagaag gtgtagaccc agttaaataa 960 <210> 79 <211> 385 <212> PRT <213> Microbulbifer degradans <400> 79 Met Pro Glu His Thr Arg Lys Arg Leu Leu Ser Thr Leu Gly Leu Ala 1 5 10 15 Leu Ser Gly Thr Ala Ile Thr Leu Thr Leu Val Gly Cys Gly Lys Asp             20 25 30 Asn Pro Ala Thr Gln Thr Glu Gly Ser His Ser Ala Gly His Thr Glu         35 40 45 Val Ala Ala Glu Gln Thr His Asp Ile Gly Gly Pro Gly Pro Glu Gly     50 55 60 Lys Pro Ile Asn Asp Pro Leu Val Thr His Ile Tyr Thr Ala Asp Pro 65 70 75 80 Ser Ala His Val Phe Asp Gly Lys Leu Tyr Ile Tyr Pro Ser His Asp                 85 90 95 Val Glu Ala Gly Ile Pro Gln Asn Asp Asn Gly Asp His Phe Asp Met             100 105 110 Arg Asp Tyr His Val Leu Ser Met Glu Glu Pro Gly Gly Lys Val Thr         115 120 125 Asp His Gly Val Ala Leu Ala Arg Glu Asp Val Ala Trp Ala Gly Arg     130 135 140 Gln Leu Trp Ala Pro Asp Ala Ala Glu Lys Asp Gly Thr Tyr Tyr Leu 145 150 155 160 Tyr Phe Pro Met Lys Asp Lys Asp Asp Ile Phe Arg Ile Gly Val Ala                 165 170 175 Ser Gly Ser Thr Pro Tyr Gly Pro Phe Lys Ala Glu Pro Glu Pro Met             180 185 190 Pro Gly Ser Tyr Ser Ile Asp Pro Ser Val Phe Gln Asp Gly Asp Asp         195 200 205 Tyr Tyr Met Tyr Ile Gly Gly Ile Trp Gly Gly Gln Leu Gln Arg Trp     210 215 220 Thr Thr Gly Glu Tyr Asn Pro Glu Asp Val Tyr Pro Ala Asp Asp Glu 225 230 235 240 Pro Ala Leu Leu Pro Lys Met Ala Lys Leu Ser Ala Asp Met Lys Ser                 245 250 255 Phe Ala Glu Pro Leu Arg Asp Ile Gln Ile Leu Asp Glu Asn Gly Glu             260 265 270 Leu Ile Lys Ala Gly Asp Asn Asp Arg Arg Phe Phe Glu Ala Ala Trp         275 280 285 Val His Lys Tyr Asn Gly Lys Tyr Tyr Leu Ser Tyr Ser Thr Gly Asp     290 295 300 Thr His Tyr Ile Val Tyr Ala Ile Gly Asp Asn Pro Tyr Gly Pro Phe 305 310 315 320 Thr Tyr Gln Gly Val Val Leu Asn Pro Val Ile Gly Trp Thr Asn His                 325 330 335 His Ser Ile Ala Glu Phe Lys Gly Lys Trp Tyr Leu Phe Tyr His Asp             340 345 350 Ser Ser Leu Ser Gly Gly Val Thr His Leu Arg Ser Val Lys Met Thr         355 360 365 Glu Leu Thr His Asn Pro Asp Gly Thr Ile Gln Thr Ile Asn Ala Tyr     370 375 380 Lys 385 <210> 80 <211> 1158 <212> DNA <213> Microbulbifer degradans <400> 80 atgccagaac atacgcgtaa gcgcttacta tcaaccttag gcctagcttt atcgggcaca 60 gctataaccc taacgcttgt ggggtgcggt aaagacaacc ccgcaactca aacagaaggc 120 agccacagcg ctggccatac agaagttgcc gcagaacaaa cacacgacat aggcggccca 180 ggccctgagg gcaagccaat taacgacccg cttgttaccc acatatacac cgcagaccct 240 tctgcccatg tgtttgacgg caaactttat atttacccat cgcacgatgt ggaagcgggt 300 attccgcaaa acgataacgg cgatcacttc gatatgcgcg attatcacgt gctttccatg 360 gaagagcctg gtggcaaagt caccgatcac ggcgtagccc ttgcgcgcga agatgtagct 420 tgggctggtc gccaactgtg ggcgcccgat gcggctgaaa aagacggcac ttactacctg 480 tatttcccca tgaaagataa ggatgacatc ttccgcattg gtgtcgccag tggcagtacc 540 ccttatggcc catttaaagc cgagccagag ccaatgcccg gcagctatag catagaccca 600 agcgtatttc aggatggcga cgactactac atgtacatag gtggtatttg gggcggccag 660 ttgcagcgtt ggacaaccgg tgagtacaac ccagaagatg tatacccagc ggatgacgag 720 cctgcgctat tacctaaaat ggccaagcta agtgcagata tgaaaagctt tgccgagcca 780 ttaagagaca ttcaaatttt ggatgaaaat ggcgagctaa ttaaagctgg cgataacgac 840 cgacgtttct tcgaagccgc gtgggtacac aaatataacg gcaagtatta cttgagctat 900 tcaaccggtg acacccacta tattgtgtat gccattggcg ataacccata cggcccgttt 960 acttaccagg gtgtagtgct caaccccgtt attggttgga ctaaccatca ctcaattgct 1020 gaatttaaag gtaagtggta tttgttctac cacgatagtt cgctttccgg tggtgtaaca 1080 catttgcgca gcgtgaaaat gacagagcta actcacaacc cagatggcac tatccaaacc 1140 attaatgcct ataagtaa 1158 <210> 81 <211> 738 <212> PRT <213> Microbulbifer degradans <400> 81 Met Ala Thr Leu Gly Val Asn Ala Ala Lys Phe Ala Met Phe Ala Ala 1 5 10 15 Ile Cys Leu Gln Phe Ser Val Ala Glu Ala Ala Lys Ser Arg Asp Gly             20 25 30 Tyr Gly Leu Trp Leu Asp Tyr Gln Pro Ile Thr Asn Thr Arg Glu Arg         35 40 45 Glu Gly Tyr Ile Lys Ala Leu Ser Pro Trp Gln Val Glu Gly Glu Ala     50 55 60 Ala Thr Ala Asp Phe Ile Arg Gln Glu Leu Thr Ala Ala Leu Gly Ala 65 70 75 80 Met Leu Gly Val Glu Ala Gly Pro Val Gly Asp Tyr Thr His Asn Ser                 85 90 95 Leu Ala His Pro Val Ala Arg Leu Leu Val Ala Thr Pro Glu Glu Ser             100 105 110 Ala Val Ile Arg Ser Leu Ala Leu Gly Asp Ala Leu Thr Arg Val Gly         115 120 125 Gln Glu Gly Tyr Leu Ile Lys Thr Thr Arg Tyr Arg Asp Lys Pro Ile     130 135 140 Thr Ile Val Thr Ala Asn Thr His Ala Gly Leu Leu Tyr Gly Thr Phe 145 150 155 160 Lys Leu Leu Gln Leu Leu Gln Thr Gly Gln Ala Val Ser Asn Leu Ala                 165 170 175 Ile Glu Ser Ala Pro Ala Thr Lys Leu Arg Val Leu Asn His Trp Asp             180 185 190 Asn Leu Asp Arg Tyr Val Glu Arg Gly Tyr Ala Gly Glu Ser Ile Trp         195 200 205 Asn Trp His Lys Leu Pro His Tyr Lys Ser Gln Arg Tyr Tyr Asp Tyr     210 215 220 Ala Arg Ala Asn Ala Ser Ile Gly Ile Asn Gly Val Val Leu Asn Asn 225 230 235 240 Val Asn Ala Asp Pro Leu Ile Leu Thr Pro Gln Tyr Leu Val Lys Val                 245 250 255 Lys Ala Leu Ala Asp Ile Phe Arg Pro Tyr Gly Ile Lys Val Tyr Leu             260 265 270 Ser Val Lys Phe Ser Ser Pro Asn Leu Ile Gly Gly Leu Pro Thr Ser         275 280 285 Asp Pro Leu Asp Lys Asn Val Gln Ala Trp Trp Gln Ala Lys Ala Asn     290 295 300 Glu Ile Tyr Ser Leu Ile Pro Asp Phe Gly Gly Phe Leu Val Lys Ala 305 310 315 320 Asn Ser Glu Gly Gln Pro Gly Pro Gly Asp Phe Gly Arg Ser His Ala                 325 330 335 Gln Gly Ala Asn Met Leu Ala Asp Ala Leu Ala Pro His Gly Gly Asn             340 345 350 Val Met Trp Arg Ala Phe Val Tyr Asn Val Glu Ala Asn Val Glu Arg         355 360 365 Ser Lys Gln Ala Tyr Asn Glu Phe Lys Pro Leu Asp Gly Thr Phe Arg     370 375 380 Gln Asn Val Leu Val Gln Val Lys Asn Gly Pro Ile Asp Phe Gln Pro 385 390 395 400 Arg Glu Pro Phe Ser Pro Leu Phe Gly Ala Met Pro Lys Thr Pro Leu                 405 410 415 Met Met Glu Phe Gln Ile Thr Gln Glu Tyr Leu Gly Phe Ser Thr His             420 425 430 Leu Val Tyr Leu Gly Pro Leu Tyr Glu Glu Val Leu Lys Ala Asp Thr         435 440 445 Tyr Ala Lys Gly Ala Gly Ser Thr Val Ala Lys Val Val Asp Gly Ser     450 455 460 Leu Tyr Gly His Gly Ile Thr Gly Met Ala Gly Val Ala Asn Ile Gly 465 470 475 480 Ser Asp Arg Asn Trp Thr Gly His Ile Phe Gly Gln Ala Asn Trp Tyr                 485 490 495 Val Phe Gly Gln Leu Ala Trp Asn Pro Glu Val Ser Thr Lys Gln Ile             500 505 510 Ala Asp Asp Trp Ile Arg Met Thr Leu Thr Arg Asp Asp Lys Ala Val         515 520 525 Asn Thr Ile Arg Ala Met Met Met Ala Ser Arg Glu Thr Ala Val Asn     530 535 540 Tyr Met Thr Pro Leu Gly Leu His His Ile Met Gly Trp Gly His His 545 550 555 560 Tyr Gly Pro Ala Pro Trp Ile Gly Glu Gln Lys Pro Asp Trp Met Arg                 565 570 575 Glu Asp Trp Thr Ser Val Tyr Tyr His Ser Ala Asn Ala Thr Gly Leu             580 585 590 Gly Lys Asp Arg Thr Ala Ser Gly Ser Asn Val Ile Ala Gln Tyr His         595 600 605 Ala Pro Leu Arg Gln Ala Tyr Ser Asp Pro Lys Thr Thr Pro Thr Glu     610 615 620 Leu Leu Leu Trp Phe His His Leu Pro Trp His Tyr Glu Leu Ala Asn 625 630 635 640 Gly Asn Ser Leu Trp His Glu Leu Val Ala Arg Tyr Tyr Leu Gly Ala                 645 650 655 Gln Ala Val Ala Glu Met Ala Lys Thr Trp Asp Gly Leu Glu Ala Asn             660 665 670 Ile Pro Pro Gln Leu Phe Lys Gln Val Gln Met Ala Leu Ala Ile Gln         675 680 685 Thr Gln Glu Ala Ala Trp Trp Arg Asp Ala Cys Val Leu Tyr Phe Gln     690 695 700 Ser Tyr Ser Lys Gln Ser Leu Pro Glu Gly Phe Ala Lys Pro Lys His 705 710 715 720 Ser Leu Glu Tyr Tyr Lys Gly Leu Ser Phe Pro His Ala Pro Gly Asp                 725 730 735 Gly arg          <210> 82 <211> 2217 <212> DNA <213> Microbulbifer degradans <400> 82 atggctactt tgggggtaaa tgccgctaag tttgccatgt ttgcagctat ttgcttgcag 60 tttagtgtcg ccgaagcggc taaaagccgc gatggatatg ggctgtggtt agattaccag 120 ccaattacca atacccgcga acgcgagggc tatataaaag cattaagccc atggcaggta 180 gaaggcgaag ctgcaactgc cgattttatt cggcaagagc ttactgcagc gttgggcgct 240 atgcttggcg ttgaggctgg tccagtgggt gattacaccc ataactccct cgctcaccct 300 gtggcgcggc tattggttgc aactccagaa gaaagcgctg ttattcgctc tttggcttta 360 ggcgatgctt taactcgagt agggcaagag gggtacctta ttaaaaccac gcgttaccgt 420 gacaagccta tcaccattgt taccgcgaac acgcatgcag gcctgctgta tggcacattc 480 aaactactgc agctgctgca aacagggcag gccgtttcta atttagctat tgagtccgcc 540 ccagcaacca aactgcgtgt gcttaaccac tgggataacc tcgatcgcta tgtggagcgc 600 ggctatgccg gtgagtctat ttggaactgg cacaagctgc cgcactacaa atcgcagcgc 660 tactacgatt acgctcgcgc taacgcgtcc attggtatta acggtgtggt actaaacaat 720 gttaacgccg accccttaat tcttaccccg cagtaccttg taaaagtaaa agcactggca 780 gatattttta ggccctacgg cattaaagtt tatctttcgg tgaagtttag ctcgccgaat 840 cttattggcg ggctgccaac atccgacccg ttagataaaa atgtgcaagc ttggtggcaa 900 gcgaaagcga atgaaattta ctcgctcatt cccgactttg gtggcttttt agtaaaagcg 960 aattcggaag ggcagcccgg cccaggggac tttggccgca gccatgcaca aggggcaaat 1020 atgttggccg atgcactggc accccatggc ggcaatgtaa tgtggcgcgc gtttgtatat 1080 aacgtagaag ccaatgtgga gcgatccaag caggcataca acgaatttaa gccattagac 1140 ggtaccttta ggcaaaacgt attggtgcaa gtaaaaaatg ggccaattga ttttcagcca 1200 cgtgaaccgt ttagcccgct gtttggtgct atgcccaaaa cgccgttaat gatggagttt 1260 caaattactc aggagtactt ggggtttagt actcaccttg tttacttggg gccgctgtac 1320 gaagaagtac ttaaggccga tacctatgcg aagggggcag ggtctactgt tgcgaaggtg 1380 gtcgatggct cgctctacgg gcacggtata acgggtatgg ctggggtagc taatattggc 1440 agcgatcgca attggaccgg ccatattttc ggccaagcca actggtatgt atttggccaa 1500 ttggcgtgga accccgaggt aagcactaag caaatagccg atgattggat tcgcatgaca 1560 ctcacccgcg acgataaagc ggtaaacacc attcgcgcaa tgatgatggc cagccgcgaa 1620 acggcggtta actacatgac gcccctgggg ctgcatcaca ttatggggtg ggggcaccac 1680 tacggcccag cgccgtggat aggcgagcaa aaacccgatt ggatgcgtga agattggaca 1740 tctgtttact atcatagcgc aaacgccaca gggctaggca aagatagaac agcttctggc 1800 agcaatgtca tagcgcaata ccacgcccct ttacggcagg cctatagcga cccgaaaacc 1860 acgcccaccg agttgctatt gtggtttcat catttgcctt ggcattatga attagcgaat 1920 ggcaatagcc tgtggcatga actggtagcg cgttactatt taggcgcgca ggctgtggca 1980 gaaatggcca aaacgtggga tggcctagaa gctaatatcc ccccgcagct attcaaacaa 2040 gtacaaatgg cgctggctat tcaaacccaa gaagccgcgt ggtggcgcga tgcctgcgtg 2100 ctgtattttc aaagctattc taagcagtcg ctacccgagg gctttgcaaa acctaagcac 2160 tcgctcgaat actataaagg gttaagcttc ccgcatgcgc cgggtgacgg gcgttaa 2217 <210> 83 <211> 1316 <212> PRT <213> Microbulbifer degradans <400> 83 Met Arg Asn Lys Leu Gly Ser Met Leu Lys Met Ser Ala Ala Ile Gly   1 5 10 15 Gly Leu Val Ala Ala Gly Ser Ala Val Ala Gly Pro Val Gly Phe Ala              20 25 30 Ser Leu Asn Gly Gly Thr Thr Gly Gly Ala Gly Gly Gln Val Val Tyr          35 40 45 Ala Ser Thr Gly Ala Glu Ile Asn Gln Ala Met Cys Asn Arg Ala Ser      50 55 60 Asp Asp Thr Pro Leu Ile Ile Tyr Val Thr Gly Thr Ile Asn His Gly  65 70 75 80 Asn Thr Ala Lys Tyr Ser Gly Ser Cys Asp Thr Thr Ala Asp Glu Ile                  85 90 95 Gln Phe Lys Gly Val Lys Asn Ile Ser Leu Ile Gly Thr Gly Ser Gly             100 105 110 Ala Val Phe Asp Gln Ile Gly Ile His Leu Arg Asp Thr Ser Asn Ile         115 120 125 Ile Leu Gln Asn Leu His Ile Lys Asn Val Lys Lys Ser Gly Ser Pro     130 135 140 Thr Ser Asn Gly Gly Asp Ala Ile Gly Met Glu Ser Gly Val Tyr Asn 145 150 155 160 Val Trp Val Asp His Cys Glu Leu Glu Ala Ser Gly Gly Glu Ser Asp                 165 170 175 Gly Tyr Asp Ser Leu Leu Asp Met Lys Ala Thr Thr Gln Tyr Val Thr             180 185 190 Val Ser Tyr Thr Tyr Tyr His Asp Ser Gly Arg Gly Gly Leu Met Gly         195 200 205 Ser Ser Asp Ser Asp Asp Thr Asn Thr Phe Val Thr Phe His His Asn     210 215 220 Tyr Tyr Glu Asn Met Asp Ser Arg Leu Pro Leu Leu Arg His Gly Thr 225 230 235 240 Ala His Ala Phe Asn Asn Tyr Tyr Asn Gly Ile Ala Lys Ser Gly Met                 245 250 255 Asn Pro Arg Ile Gly Gly Gln Ile Lys Ala Glu Asn Asn Tyr Phe Glu             260 265 270 Asn Ala His Asn Pro Ile Gly Thr Phe Tyr Thr Asp Asp Met Gly Tyr         275 280 285 Trp Asp Leu Arg Gly Asn Ile Phe Gly Ser Asn Val Thr Trp Ala Ser     290 295 300 Ala Asp Asp Glu Thr Pro Ala Gly Pro Asn Pro Thr Ser Thr Thr Ser 305 310 315 320 Ile His Ile Ser Tyr Pro Tyr Asp Leu Asp Asp Ala Ala Cys Val Pro                 325 330 335 Asp Ile Val Lys Ser Thr Ala Gly Val Gly Thr Gly Leu Ala Val Ser             340 345 350 Asp Gly Ser Cys Thr Ile Thr Thr Pro Pro Ser Thr Ser Ser Ser Ser         355 360 365 Ser Ser Ser Ser Ser Thr Ser Ser Thr Gly Ser Ser Ser Ser Ser Ser     370 375 380 Ser Ser Ser Ser Ser Ser Ser Ser Ser Asn Gly Ser Leu Val Leu 385 390 395 400 Gly Asn Asn Leu Ser Ile Gly Ala Gly Ser Asp Gly Ser Ser Lys Gly                 405 410 415 Ala Gly Ser Tyr Gly Asn Val Arg Asp Gly Asp Val Ser Ser Tyr Trp             420 425 430 Ala Pro Ser Gly Ser Thr Gly Arg Val Ser Ile Lys Trp Ser Gly Ser         435 440 445 Gln Thr Val Asn Ala Ile Val Ile Lys Glu Ala Ala Gly Tyr Glu Gly     450 455 460 Asn Ile Ser Gly Trp Gln Val Thr Asp Asn Asp Thr Gly Ala Val Leu 465 470 475 480 Ala Ala Gly Ser Ser Val Gly Thr Ile Thr Phe Asp Ala Val Thr Thr                 485 490 495 Ser Lys Ile Asn Phe Glu Ile Thr Ser Ser Asn Gly Thr Pro Thr Val             500 505 510 Ala Glu Phe Glu Thr Tyr Asn Ala Thr Gly Ser Ser Ser Ser Ser Ser         515 520 525 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser     530 535 540 Ser Ser Ser Ser Ser Ser Ser Thr Gly Gly Thr Ala Thr Leu Ser Thr 545 550 555 560 Thr Val Ser Gly Asp Gln Val Thr Leu Asn Trp Ser Val Asn Asn Ala                 565 570 575 Thr Val Thr Gly Gln Gln Ile Tyr Arg Asp Val Asp Ser Asp Pro Ala             580 585 590 Gly Arg Val Arg Ile Ala Ser Gly Val Thr Gly Asn Thr Tyr Thr Asp         595 600 605 Thr Gly Leu Ala Asn Gly Thr Tyr Tyr Tyr Trp Val Lys Val Thr Asp     610 615 620 Ser Asn Ser Ala Thr Ile Asn Ser Asn Tyr Ser Glu Ala Gln Val Asn 625 630 635 640 Val Tyr Thr Thr Ser Thr Thr Thr Phe Glu Glu Asp Ala Gly Tyr Cys                 645 650 655 Ser Val Asp Gly Ser Val Asp Ser Asn Asn Ser Gly Phe Ala Gly Ser             660 665 670 Gly Phe Ala Asn Thr Asp Asn Ala Ser Gly Asn Gly Val Asn Tyr Ala         675 680 685 Val Ser Val Pro Val Ala Gly Val Tyr Thr Leu Gln Val Arg Phe Ala     690 695 700 Asn Gly Ser Ser Ala Arg Pro Ala Asp Val Leu Val Asn Tyr Gly Asn 705 710 715 720 Ala Gly Val Phe Asp Leu Pro Ser Thr Gly Ser Trp Thr Ser Trp Ser                 725 730 735 Asn Ser Asn Glu Ile Ser Val Asn Leu Val Ala Gly Asn Asn Ile Ile             740 745 750 Arg Leu Glu Ala Thr Thr Ser Gly Gly Leu Ala Asn Ile Asp Ser Leu         755 760 765 Ser Val Thr Gly Val Glu Pro Ser Ala Gly Asp Cys Asn Gly Ser Val     770 775 780 Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Thr Ser 785 790 795 800 Ser Ser Ser Thr Ser Ser Gly Gly Ser Ser Thr Ser Ser Ser Ser Thr                 805 810 815 Ser Ser Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser Thr Ser Ser Ser             820 825 830 Ser Thr Ser Ser Thr Ser Ser Ser Ser Gly Gly Gly Thr Ala Ser Cys         835 840 845 Glu Gln Leu Ile Asn Asp Pro Ser Val Asn Trp Asp Glu Ser Ala Leu     850 855 860 Ala Ser Glu Gln Glu Ile Val Ala Cys Leu Ala Gln Ser Leu Gly Ser 865 870 875 880 Pro Val Gly Phe Gly Glu Gly Thr Thr Gly Gly Tyr Asp Pro Ser Gly                 885 890 895 Gly Ser Asn Leu Val Val Ile Lys Lys Asn Ile Gly Ile Ser Val Glu             900 905 910 Gln Gln Ile Leu Asp Ala Ile Ser Thr Glu Asn His Asn Trp Ile Val         915 920 925 Phe Asp Lys Asp Asp Phe Ala Ala Arg Thr Ala Val Ala Met Tyr Arg     930 935 940 Leu Asp Cys Asp Asn Ala Asp Val Arg Ser Ala Leu Gly Gly Ala Ser 945 950 955 960 Ala Ala Gln Cys Arg Asp His Ile Ala Trp Cys Ser Ala Asn Gly Ile                 965 970 975 Ser Asp Glu His Asp Cys Glu Asn Glu Phe Phe Asn Asn Arg Leu Asn             980 985 990 Asp Ser Asp Leu Pro Ile Arg Asn Gln Met Ile Gln Ser Asn Thr Thr         995 1000 1005 Ile Asp Gly Arg Gly Ala Asn Ala Tyr Phe Phe Phe Asn Gly Phe Ser    1010 1015 1020 Ile Gly Lys Asp Ser Ser Gly Ala Ser Leu Tyr Ala Ala Gln Asn Val 1025 1030 1035 1040 Ile Val Thr Asn Asn Glu Phe Ile Gly Ala Gly His Thr Glu Asp His                1045 1050 1055 Asp Leu Asp Pro Asp Met Ile Arg Ser Thr Gly Glu Ser Asn Lys Ile            1060 1065 1070 Trp Ile His Gln Asn Thr Phe Asp His Thr Gly Asp Ser Ala Phe Asp        1075 1080 1085 Val Lys Val Gly Ala Tyr Asp Ile Thr Ile Ser Phe Asn Lys Leu Val    1090 1095 1100 Asn Val Lys Arg Ala Ala Leu His Gly Ser Ser Asp Ser Arg Ala Ile 1105 1110 1115 1120 Asn Ser Gln Ile Thr Thr Thr Met His Asn Asn Leu Phe Tyr Thr Ser                1125 1130 1135 Asp Asp Gln Tyr Ala Leu Ser Thr Tyr Asp Thr Leu Arg Arg Val Pro            1140 1145 1150 Leu Met Arg Arg Gly Gln Ser His Met Phe Asn Asn Val Phe Tyr Gly        1155 1160 1165 Tyr Arg Lys Asp Ile Leu Ser Val Arg Val Gly Gly Arg Ile Ala Phe    1170 1175 1180 Glu Asp Asn Ile Ile Leu Asn Lys Glu Ser Ser Ser Thr Pro Gly Asp 1185 1190 1195 1200 Gly Leu Lys Lys Gly Asp Asp Met Glu Tyr Tyr Val Glu Thr Leu Leu                1205 1210 1215 Arg Asp Phe Arg Glu Gly Gly Leu Glu Ile Ser Gly Ser Tyr Val Ser            1220 1225 1230 Phe Ala Asp Ser Ala Cys Asn Ser Tyr Gly Ala Ser Gly Asp Leu Thr        1235 1240 1245 Ala Ser His Gly Ala Thr Pro Asp Met Phe Asp Asp Tyr Ser Ser Ala    1250 1255 1260 Ser Lys Asn Thr Ile Ser Ala Asn Arg Phe Val Ala Gly Asp Asp Leu 1265 1270 1275 1280 Thr Asp Tyr Val Phe Ala Thr Ala Gly Lys Gly Gly Lys Ala Pro Tyr                1285 1290 1295 Val Ser Thr Phe Thr Ala Gly Gln Asn Ser Leu Ile Ser Gln Ala Asn            1300 1305 1310 Pro Val Cys Gln        1315 <210> 84 <211> 93 <212> PRT <213> Microbulbifer degradans <400> 84 Met Pro Pro Val Glu Leu Leu Asp Glu Leu Leu Asp Glu Glu Leu Asp 1 5 10 15 Glu Leu Leu Asp Glu Glu Leu Glu Glu Leu Glu Leu Leu Glu Glu Leu             20 25 30 Glu Pro Val Ala Leu Tyr Val Ser Asn Ser Ala Thr Val Gly Val Pro         35 40 45 Leu Glu Glu Val Ile Ser Lys Leu Ile Leu Leu Val Val Thr Ala Ser     50 55 60 Asn Val Ile Val Pro Thr Leu Glu Pro Ala Ala Asn Thr Ala Pro Val 65 70 75 80 Ser Leu Ser Val Thr Cys Gln Pro Leu Ile Leu Pro Ser                 85 90 <210> 85 <211> 3951 <212> DNA <213> Microbulbifer degradans <400> 85 atgagaaata aattaggctc aatgttaaaa atgagcgcag ccattggcgg tttagttgca 60 gcgggttccg ctgttgcagg cccagttggt ttcgcaagtt taaacggcgg cactaccggc 120 ggcgcgggcg ggcaagttgt atatgctagc accggtgctg aaattaacca ggctatgtgt 180 aatcgcgcaa gcgacgatac accgctaatt atttatgtga cgggtaccat taaccacggt 240 aacaccgcca agtattctgg tagctgcgat accactgcag atgaaattca gtttaaaggt 300 gtaaaaaata tatcgttgat aggaacgggc agcggtgctg tgttcgatca aatcggtatt 360 cacctacgcg atacctcgaa tattattttg caaaatttgc atattaaaaa cgttaagaag 420 tctggttcgc ctacttcgaa tggcggtgac gctattggta tggaatctgg cgtatacaat 480 gtgtgggtag accactgtga gctagaagct tcaggcggtg aaagtgatgg atatgattca 540 ttgctagata tgaaagccac cacgcagtat gtaacggttt cttacactta ctatcacgat 600 tctggtcgcg gtggtttaat ggggtctagt gatagcgacg ataccaatac cttcgtcacc 660 ttccaccaca actactacga aaatatggat tcgcgcttgc cgttactgcg tcacggtaca 720 gctcatgcat ttaacaacta ctataatggt attgctaaat ctggcatgaa cccacgtata 780 ggtgggcaaa taaaagcgga aaacaattac ttcgaaaatg cgcacaaccc aattggtact 840 ttttatacag acgatatggg ttactgggac ttacgcggca atatatttgg cagtaacgta 900 acgtgggcgt ctgcggatga tgaaacccct gcaggcccga acccaacatc cactacgtct 960 attcatattt cttaccccta tgatctagat gacgctgctt gtgtgcctga tattgtaaaa 1020 tccacagcag gtgtgggtac tggcctagcg gtttcagacg gaagctgcac cataacaacg 1080 ccaccttcaa cgagttcgtc tagctccagt tctagctcaa cctcgtcgac tggttcgagt 1140 tcgtcttcaa gctcttcctc ttcaagcagc tctagctcca atggcggcag cttagtatta 1200 ggtaacaacc tttcaattgg tgctggctct gatggtagta gcaagggagc aggttcgtac 1260 ggcaatgtgc gcgatggcga tgtaagtagc tattgggcgc cgagtggcag tactggtcgt 1320 gtttcaatta aatggagcgg cagccaaact gttaacgcta ttgttattaa agaagcggca 1380 ggctatgaag gtaatattag tggttggcaa gtaactgata acgataccgg tgcagtattg 1440 gctgctggct caagcgtagg cacaattacg tttgatgcgg taacgactag caagatcaat 1500 ttcgaaatta cttcttctaa cggtacacca acggtagcgg aattcgaaac atataatgct 1560 acaggttcta gctcttcaag cagctcgagt tcttctagct cttcatcaag tagctcgtct 1620 agttcttcat cgagcagttc gtctagtagt tctacaggcg gcaccgctac tttaagtaca 1680 acggtttctg gcgatcaagt aacgttgaat tggagcgtaa ataatgcaac cgtaactggt 1740 cagcaaattt atcgcgatgt ggattcagac ccagctggcc gtgtgcgcat tgcatccggt 1800 gtaactggaa atacttacac agataccggt ttggctaacg gaacttatta ctactgggta 1860 aaagtaaccg attcaaactc agctacaatt aattccaact actcagaagc gcaagtgaat 1920 gtttatacaa catctactac aacgtttgaa gaggatgcgg gttattgctc ggtagacggt 1980 tcagtagata gcaataacag tggctttgct ggcagtggtt ttgctaatac cgataatgct 2040 tcgggtaatg gcgtaaacta cgcggtaagc gtacccgttg ctggtgtgta cacgctgcaa 2100 gtgcgttttg ctaatggctc aagtgcacgt cctgctgatg tgttagtgaa ctatggtaac 2160 gccggtgtat ttgatctgcc tagcacaggt tcttggacca gctggagcaa ctcaaacgaa 2220 attagcgtta acttagttgc tggcaataat attattcgtt tagaggctac cacgtctggc 2280 ggcttggcga atattgatag cctatctgta acgggtgtag agccttctgc aggtgactgt 2340 aacggtagtg ttggttctag cagttctagt tcttccagct cttctacttc tagcaccagt 2400 tcatctagca ctagctctgg tggcagctct actagctcaa gctcaacgtc tagctcatct 2460 acaagctcta catctagtag ttcaaccagc tctagcagca cgtcttctac ctcaagctct 2520 tcgggcggtg gtacggcaag ttgtgagcag ttgattaacg atccaagtgt taactgggat 2580 gagtctgcac tggcttcaga gcaagaaatt gtagcctgtt tggctcagtc tctaggtagc 2640 cctgttggct ttggggaagg tactaccggt ggttacgatc caagtggcgg cagcaacctt 2700 gttgttatta aaaagaacat aggtatttct gttgagcaac aaattttgga tgctataagc 2760 accgaaaacc acaactggat tgtgttcgac aaagatgatt ttgctgcgcg cactgcggta 2820 gcgatgtatc gcttagattg tgacaatgcc gatgtgcgtt cagcattggg tggcgcaagt 2880 gctgcacaat gtcgcgatca tatagcttgg tgttctgcta atggtatttc tgacgagcat 2940 gactgtgaaa atgaattctt taacaaccgt ttaaatgatt cagatttgcc aatccgcaat 3000 caaatgattc agtcaaacac taccattgat ggtcgtggtg caaacgcata cttcttcttt 3060 aatggtttct ccattggtaa agatagcagt ggtgcaagct tgtacgcagc gcaaaatgtg 3120 attgtaacga ataacgagtt tattggtgcc ggtcacactg aagatcacga tctagaccca 3180 gatatgattc gatctactgg cgaatcgaac aaaatttgga ttcaccaaaa cacgttcgac 3240 catactggtg attctgcgtt tgacgtaaag gtgggtgctt acgatataac aatatcattc 3300 aataagttgg tgaacgtgaa gcgtgctgcg ctacatggtt caagtgatag ccgagcaatt 3360 aactcgcaaa tcacaaccac tatgcacaac aacctgttct atacttcaga tgatcaatac 3420 gcgctaagta cctacgacac tttgcgtcgt gtaccgctaa tgcgtcgcgg tcaatcacac 3480 atgtttaaca acgttttcta cggttaccgt aaagatattc taagcgtgcg tgttggcggt 3540 cgtatcgcct ttgaagataa cattattttg aataaagaaa gcagctctac cccaggtgat 3600 ggcctgaaga aaggcgacga catggaatac tatgttgaaa ccttgttgcg cgacttccgt 3660 gagggtgggt tagaaattag cggtagctat gtatcgtttg cagatagcgc ttgtaattcc 3720 tatggcgcat cgggtgactt aaccgcatcg catggtgcta cgccagatat gtttgatgat 3780 tacagctctg catctaaaaa tactatatca gccaatcgct ttgttgctgg cgatgactta 3840 actgactatg tatttgctac tgcaggtaag ggcggtaaag cgccttatgt ttccaccttt 3900 actgctgggc aaaatagcct tatttcacag gctaacccag tttgtcagta g 3951 <210> 86 <211> 427 <212> PRT <213> Microbulbifer degradans <400> 86 Met Asn Lys Asn Asn Val Ile Ala Tyr Leu Leu Ile Ser Thr Phe Leu 1 5 10 15 Leu Phe Ser Ala Thr Val Phe Ala Val Lys Pro Ser Asn Ala Glu Thr             20 25 30 Arg Tyr Ser Ala Met Gly Ala Asp Thr Pro Ala Gly Leu Gly Gly Thr         35 40 45 Leu Pro Asp Gly Gln Ser Arg Ile Val Arg Val Thr Asn Leu Asn Ala     50 55 60 Ser Gly Glu Gly Ser Leu Ala Trp Ala Leu Gly Leu Ala Arg Pro Arg 65 70 75 80 Val Val Val Phe Glu Val Gly Gly Val Ile Asp Leu Ala Gly Gln Ser                 85 90 95 Ile Thr Val Thr Gln Pro Phe Leu Thr Val Ala Gly Gln Ser Ala Pro             100 105 110 Ala Pro Gly Ile Thr Leu Ile Arg Gly Gly Leu Asn Ile Arg Thr His         115 120 125 Asp Val Arg Val Gln His Ile Arg Val Arg Pro Gly Asp Asn Leu Gln     130 135 140 Pro Lys Arg Ser Gly Trp Glu Ser Asp Gly Ile Ser Val Ala Gly Glu 145 150 155 160 Asn Ala Lys Asp Val His Ile Asp His Val Ser Val Ser Trp Ala Val                 165 170 175 Asp Glu Asn Leu Ser Ala Ser Gly Asn Arg Tyr Lys Gly Tyr Gly Gln             180 185 190 Thr Ala Glu Arg Val Thr Phe Ser Asn Asn Leu Ile Ala Glu Ala Leu         195 200 205 Asp Tyr Ala Ser His Lys Lys Gly Lys His Ser Lys Gly Leu Leu Val     210 215 220 His Asp Tyr Val Arg Asp Val Ala Val Val Arg Asn Leu Phe Val Ser 225 230 235 240 Asn Asp Arg Arg Asn Pro Tyr Phe Lys Ala His Thr Ile Gly Phe Val                 245 250 255 Ala Asn Asn Ile Ile Tyr Asn Ala Gly Asn Ala Ala Ile Gln Val Asn             260 265 270 Tyr Ile Glu Arg Glu Trp Glu Gly Gln Ser Thr Gly Pro Ala Asn Ala         275 280 285 Arg Val Ala Val Val Asn Asn Gln Leu Val Tyr Gly Arg Asp Thr Tyr     290 295 300 Ser Asp Leu Ala Leu Val Ser Val Arg Gly Asp Ala Tyr Leu Thr Gly 305 310 315 320 Asn Ser Val Thr Asn Leu Met Gly Glu Pro Met Pro Ile Thr Glu Gly                 325 330 335 Ala Val Asn Ser Leu Ala Ser Ala Pro Ser Trp Leu Thr Gly Tyr Glu             340 345 350 Leu Trp Asp Ala Asp Glu Met Arg Glu Leu Leu Val Ala Ser Val Gly         355 360 365 Ala Thr Pro Trp Ala Arg Asp Ala Ile Asp Thr Arg Ile Ile Asn Gly     370 375 380 Val Ala Thr Gly Lys Ala Arg Ile Ile Asp Ser Gln Gln Asp Val Gly 385 390 395 400 Gly Tyr Pro Ser Tyr Lys Gln Thr Asn Lys Lys Phe Asp Ile Pro Asp                 405 410 415 Asp Lys Ile Ala Glu Trp Leu Leu Gly Tyr Leu             420 425 <210> 87 <211> 1284 <212> DNA <213> Microbulbifer degradans <400> 87 atgaataaaa ataatgtaat tgcttatctg ctaatttcaa cttttctatt attttctgcg 60 actgtgttcg cggttaaacc cagcaacgct gaaacccgat attccgcaat gggtgcagat 120 accccagcag gtttgggagg cactttgcca gatggtcagt ctcgtatcgt tagggtgact 180 aatttaaatg caagtgggga gggctcgctc gcatgggcgc tgggtttagc tcgaccacgc 240 gtagtggtgt tcgaagttgg cggtgttata gatcttgctg ggcaaagtat taccgtcacg 300 cagccattcc ttactgttgc cggtcagtcg gcgccagcac cgggtattac attaattcgc 360 ggcggtttaa atatacgaac ccacgatgta agagtgcagc atattcgcgt gcggccggga 420 gataacttac aaccaaagcg ctccggctgg gaaagtgacg gtatatctgt ggccggtgaa 480 aatgccaaag atgtacatat agatcatgta tcggtaagtt gggcggtaga tgaaaacctc 540 tccgcttcgg ggaatcgtta caaaggttac ggtcaaaccg ctgagcgggt aacgtttagt 600 aataatctca ttgccgaagc gttagattat gccagccata aaaaaggcaa acactctaag 660 ggattattgg tacacgatta tgtgcgagat gttgccgtag ttagaaattt gtttgtgtct 720 aatgatcgtc gcaacccgta ctttaaagcg cacaccatag gttttgtagc aaataatatt 780 atttacaatg cgggtaatgc cgctatacag gttaactata ttgagcgtga gtgggagggc 840 cagagtacag gcccagctaa tgctagagta gcggtggtaa ataaccagtt agtttacggc 900 cgcgatacat actcagactt ggcgctagtg tctgtgcgtg gggatgctta tttgacgggt 960 aatagcgtta caaatttaat gggcgagccc atgcctatta cggaaggggc ggttaattct 1020 ttagcctctg caccttcatg gttaacaggt tatgagttgt gggatgctga cgagatgcgt 1080 gagctgctag tagccagtgt tggtgcaaca ccctgggcca gggatgcgat agatacccga 1140 ataattaatg gggtggcaac ggggaaggcg cgaataatag atagccagca agatgtgggt 1200 ggctacccga gctataagca aacaaataaa aaatttgata taccagacga caaaattgcc 1260 gaatggttac tgggttacct gtaa 1284 <210> 88 <211> 769 <212> PRT <213> Microbulbifer degradans <400> 88 Met Arg Asn Thr Lys His Leu Leu Asn Ser Gly Ala Val Leu Leu Ala 1 5 10 15 Ser Ser Ile Ala Thr Ala Ala Met Ala Gly Pro Val Gly Phe Ala Ser             20 25 30 Leu Asn Gly Gly Thr Thr Gly Gly Gln Gly Gly Gln Val Val Tyr Ala         35 40 45 Asn Thr Gly Thr Gln Ile Asn Glu Ala Met Cys Asn Arg Pro Ser His     50 55 60 Asp Thr Pro Leu Ile Ile Tyr Val Ser Gly Thr Ile Asn His Gly Asn 65 70 75 80 Thr Glu Lys Val Ser Gly Asn Cys Asp Thr Thr Gly Asp Glu Ile Gln                 85 90 95 Phe Lys Lys Val Lys Asn Leu Ser Leu Ile Gly Thr Gly Asn Gly Ala             100 105 110 Val Phe Asp Gln Ile Gly Ile His Leu Arg Glu Thr Ser Asn Ile Ile         115 120 125 Leu Gln Asn Leu His Ile Lys Asn Val Lys Lys Ser Gly Ser Pro Thr     130 135 140 Ser Asn Gly Gly Asp Ala Ile Gly Met Glu Ser Gly Val Tyr Asn Val 145 150 155 160 Trp Val Asp His Cys Glu Leu Glu Ala Ser Gly Gly Glu Lys Asp Gly                 165 170 175 Tyr Asp Ser Leu Leu Asp Met Lys Ala Thr Thr Gln Tyr Val Thr Val             180 185 190 Ser Tyr Thr Tyr Tyr His Asp Ser Gly Arg Gly Gly Leu Met Gly Ser         195 200 205 Ser Asp Ser Asp Asp Thr Asn Thr Tyr Val Thr Phe His His Asn Tyr     210 215 220 Tyr Lys Asn Met Asp Ser Arg Leu Pro Leu Leu Arg His Gly Thr Ala 225 230 235 240 His Ala Phe Asn Asn Tyr Tyr Asp Gly Ile Thr Lys Ser Gly Met Asn                 245 250 255 Pro Arg Ile Gly Gly Gln Ile Lys Ala Glu Asn Asn Tyr Phe Glu Asn             260 265 270 Ala His Asn Pro Ile Gly Thr Phe Tyr Thr Asn Asp Met Gly Tyr Trp         275 280 285 Asp Leu Ser Gly Asn Ile Phe Gly Asn Asn Val Thr Trp Ala Ser Ala     290 295 300 Asp Asp Glu Thr Pro Ala Gly Pro Asn Pro Gln Ser Thr Thr Ser Ile 305 310 315 320 His Ile Ser Tyr Pro Tyr Ser Leu Asp Asp Ala Thr Cys Val Pro Lys                 325 330 335 Ile Val Lys Ala Thr Ala Gly Val Gly Asn Gly Leu Ala Val Ser Thr             340 345 350 Gly Gly Ser Asn Cys Gly Thr Ser Ser Ser Ser Ser Ser Ser Ser         355 360 365 Ser Ser Ser Thr Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser Ser Ser     370 375 380 Ser Asn Ser Ser Ser Gly Gly Ser Gly Val Asn Leu Ser Ile Gly Ala 385 390 395 400 Gly Ser Asp Gly Ser Ser Lys Gly Ala Gly Ser Tyr Gly Asp Val Arg                 405 410 415 Asp Gly Asn Met Ser Thr Tyr Trp Ala Pro Ser Gly Ser Thr Gly Arg             420 425 430 Val Ser Ile Lys Trp Ser Ser Ala Thr Thr Val Ser Ser Ile Val Ile         435 440 445 Lys Glu Ala Ala Gly Phe Glu Gly Asn Ile Thr Gly Trp Gln Val Val     450 455 460 Asn Asn Glu Asn Gly Ala Val Leu Lys Ser Gly Ser Asn Ala Gly Val 465 470 475 480 Ile Ser Phe Ser Pro Val Ser Thr Thr Lys Leu Asn Phe Glu Ile Thr                 485 490 495 Ser Ser Asn Gly Met Pro Thr Val Ala Glu Phe Glu Thr Tyr Ser Gly             500 505 510 Thr Val Gly Gly Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser         515 520 525 Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Gly Gly Ser Ala     530 535 540 Asn Leu Gly Thr Ser Val Ser Gly Asp Gln Val Ser Leu Asn Trp Ser 545 550 555 560 Thr Ser Asn Ile Asp Val Gly Ser Gln Gln Val Tyr Arg Asp Thr Asp                 565 570 575 Ser Asn Pro Ser Gly Arg Val Arg Ile Ser Ala Gly Val Ser Gly Asn             580 585 590 Ser Tyr Thr Asp Tyr Gly Leu Ala Ser Gly Thr Tyr Tyr Tyr Trp Ile         595 600 605 Lys Ile Thr Asp Gln Asn Gly Val Val Tyr Asn Thr Asn Ala Ala Glu     610 615 620 Ala Val Val Gly Ser Gln Ala Pro Thr Thr Phe Thr Ala Gln Glu Ser 625 630 635 640 Ala Gly Phe Cys Ser Val Asn Gly Ser Val Asp Ser Asn Asn Ala Gly                 645 650 655 Tyr Thr Gly Asp Gly Phe Val Asn Thr Asp Asn Ala Ser Gly Asn Ala             660 665 670 Ala Val Tyr Ala Phe Asn Ala Pro Ser Ala Gly Met Tyr Ser Leu Gln         675 680 685 Val Arg Tyr Ala Asn Gly Ser Ser Ala Arg Pro Gly Asp Val Leu Val     690 695 700 Asn Ala Gly Asn Ile Gly Thr Phe Asp Phe Ser Ser Thr Gly Ser Trp 705 710 715 720 Thr Ser Trp Ala Asn Ser Asn Glu Leu Ser Ala Tyr Phe Ser Ala Gly                 725 730 735 Asn Asn Thr Val Arg Ile Gln Ala Thr Asn Ser Gly Gly Leu Pro Asn             740 745 750 Leu Asp Ser Val Ser Val Thr Gly Asn Ala Pro Ala Ala Gly Asn Cys         755 760 765 Asn      <210> 89 <211> 2310 <212> DNA <213> Microbulbifer degradans <400> 89 atgagaaaca ctaaacacct actcaatagc ggtgccgtat tgctagctag cagtattgca 60 acagctgcga tggcggggcc tgtgggcttt gcatcgctta atggtggcac aacgggcggt 120 caaggcggtc aagttgtata cgccaacacc ggtacacaaa ttaacgaagc catgtgtaac 180 cgcccatctc acgatacgcc gttaattatt tatgtatccg gtaccattaa ccacggcaac 240 accgaaaagg tgtcgggtaa ttgcgataca accggcgacg agattcagtt taaaaaagtt 300 aaaaacctat cgttaattgg tactggtaac ggtgcggtgt ttgatcaaat aggtattcat 360 ttacgcgaaa cctccaatat tattctgcaa aaccttcata ttaaaaatgt taaaaaatcg 420 ggttctccaa cctccaatgg cggcgatgca attggaatgg agtctggcgt atacaatgtg 480 tgggtggatc actgtgagct agaagcatcc ggtggtgaaa aagatggtta cgattcattg 540 ctagatatga aagcaaccac gcagtatgta accgtttctt acacctatta ccacgattca 600 ggccgcggtg gtttaatggg gtcgagcgat agtgacgata ccaacaccta cgtgactttc 660 caccacaatt actacaaaaa tatggattca cgcttaccgc ttttacgcca cggtactgcg 720 catgccttta acaactatta cgatggcatt accaaatctg gtatgaaccc ccgtataggc 780 ggtcaaataa aagcagaaaa taactatttc gaaaacgcac acaacccaat aggtacgttt 840 tacacaaacg atatgggtta ctgggactta agcggcaata tatttggcaa caacgtaacg 900 tgggcgtctg cggatgatga aacccctgca gggccgaatc cacaatccac aacgtccatt 960 catatttctt acccctacag cttggatgac gcaacgtgcg tgccgaagat tgtaaaagct 1020 actgcgggtg tgggtaacgg tttggctgtg tctaccggtg gtagcaattg cggtacttct 1080 agctcttcgt ctagcagctc ttcgtccagc tctacctcgt ctaccagttc tacatctagc 1140 agttcttcat catccaatag ttcttctggt ggctcaggtg taaacctttc aattggcgca 1200 ggttctgatg gtagcagtaa aggtgcgggc tcttatggcg atgtgcgcga tggcaatatg 1260 agcacctact gggcaccgag tggcagcact ggtcgcgtat ctattaagtg gagttcggca 1320 acgacggtaa gtagcattgt tattaaagaa gcggctggct ttgaaggtaa cattactggc 1380 tggcaagttg taaacaacga gaatggcgca gtattaaaaa gcggctctaa cgctggcgta 1440 atttcttttt ctccggtttc tactacgaag ttaaatttcg aaattacctc ttcgaacggc 1500 atgcccacgg ttgcagaatt tgaaacctat agcggcacgg ttggtggtac ttcgtcatcc 1560 agttcttcaa gtagttcgtc aagcagttct tcaagcagct caagttcaac gagcagctct 1620 ggtggttccg cgaatctagg tacctcggta agtggcgatc aggtttcact taattggtct 1680 acctcaaaca ttgatgtggg ttcgcagcaa gtttatcgcg ataccgattc aaacccatct 1740 ggtcgtgtgc gtatctctgc aggcgtttct ggtaattcgt ataccgatta cggcttagct 1800 agcggtactt attactactg gataaaaatt accgatcaaa acggtgttgt ttacaacaca 1860 aatgccgcag aagcggttgt aggcagccaa gcgccaacga cgtttactgc gcaagagtct 1920 gcgggtttct gctctgttaa cggttctgtt gattccaata acgctggcta cactggcgat 1980 ggttttgtaa ataccgataa cgccagtggc aatgcagcag tttatgcctt caatgcacct 2040 agtgcgggta tgtatagctt gcaggttcgc tacgcaaacg gttctagtgc acgcccaggt 2100 gatgtgctgg tcaacgctgg caatattgga acatttgatt tttccagtac cggttcttgg 2160 acatcttggg caaacagtaa tgagttaagt gcgtacttct ctgcgggtaa caatactgtt 2220 cgtattcaag ctactaactc tggcggctta cctaacttag atagtgtttc tgtaacgggt 2280 aatgcaccag cggcaggcaa ctgtaattaa 2310 <210> 90 <211> 594 <212> PRT <213> Microbulbifer degradans <400> 90 Met Lys Ile Phe Lys Leu Leu Leu Met Phe Val Leu Ala His Asn Leu 1 5 10 15 Val Ala Cys Gly Gly Ser Asn Asp Gly Gly Glu Ile Glu Leu Asn Phe             20 25 30 Gly Glu Glu Asn Thr Pro Glu Pro Glu Thr Glu Pro Glu Ala Glu Pro         35 40 45 Glu Gly Glu Pro Glu Gly Glu Pro Glu Gly Glu Pro Glu Gly Glu Pro     50 55 60 Glu Gly Glu Pro Glu Gly Glu Thr Ala Asp Ala Thr Ala Asp Ala Gly 65 70 75 80 Phe Ala Gly His Asn Phe Asn Leu Thr Gly Gly Glu Gly Gly Thr Ala                 85 90 95 Tyr Thr Val Asn Asn Gly Lys Asp Leu Gln Thr Val Leu Asp Asn Ala             100 105 110 Lys Ser Ser Asn Ser Pro Val Ile Ile Tyr Val Asp Gly Thr Ile Asn         115 120 125 Ser Phe Asn Ser Ala Asn Gly Asn Gln Pro Ile Gln Ile Lys Asp Met     130 135 140 Asp Asn Val Ser Ile Ile Gly Tyr Gly Ala Glu Ala Thr Phe Asp Gly 145 150 155 160 Val Gly Ile Ala Ile Arg Arg Ala Asn Asn Ile Ile Ile Arg Asn Leu                 165 170 175 Thr Phe Lys Ser Val Leu Thr Glu Gly Lys Asp Ala Ile Ser Ile Glu             180 185 190 Gly Asp Asp Asp Gly Ser Thr Thr Ser Asn Ile Trp Val Asp His Asn         195 200 205 Glu Phe Tyr Ser Ala Pro Thr Ala Asp Lys Asp Phe Tyr Asp Gly Leu     210 215 220 Ile Asp Ser Lys Ser Gly Ala Ser Asn Ile Thr Ile Ser Tyr Asn Tyr 225 230 235 240 Leu His Asp His Trp Lys Ala Ser Leu His Gly His Thr Glu Asn Asp                 245 250 255 Glu Gly Ala His Asn Thr Asp Arg Lys Ile Thr Phe His His Asn Arg             260 265 270 Phe Glu Asn Ile Glu Ser Arg Leu Pro Leu Phe Arg Arg Gly Val Gly         275 280 285 His Leu Tyr Asn Asn Tyr Tyr Lys Asp Val Gly Ser Thr Ala Ile Asn     290 295 300 Ser Arg Ile Gly Ala Glu Leu Leu Ile Glu Asn Asn Val Phe Glu Asp 305 310 315 320 Ser Gln Asn Pro Ile Val Ser Phe Tyr Ser Asp Val Ile Gly Tyr Trp                 325 330 335 Asn Thr Ser Gly Asn Leu Phe Thr Asn Val Thr Trp Thr Thr Pro Gly             340 345 350 Thr Gly Glu Val Ser Ala Gly Ala Thr Gln Thr Pro Thr Ser Asp Tyr         355 360 365 Val Val Pro Tyr Ser Tyr Thr Leu Met Pro Ala Ala Asp Val Lys Ala     370 375 380 His Val Ile Ala Ser Ala Gly Val Gly Lys Ile Asp Gln Thr Gly Leu 385 390 395 400 Thr Ile Pro Asp Pro Val Thr Pro Glu Gly Asp Leu Gly Glu Pro Glu                 405 410 415 Ala Pro Val Gln Gly Asp Val Ser Leu Pro Tyr Thr Glu Asn Phe Ala             420 425 430 Ala Thr Asp Ala Ala Asn Phe Phe Ser Ala Ala Tyr Arg Asp Ile Thr         435 440 445 Gly Ser Ala Gly Thr Ser Thr Pro Met Tyr His Arg Val Thr Gly Thr     450 455 460 Val Glu Ile Asn Ala Gln Gln Leu Asp Met Thr Gly Ala Arg Val Ser 465 470 475 480 Ile Gly Asn Thr Thr Pro Ser Val Ser Thr Thr Gly Ala Asp Thr Thr                 485 490 495 Thr Thr Gly Val Leu Asp Leu Ser Ala Pro Tyr Thr Val Ser Phe Lys             500 505 510 Val Val Ser Val Gly Gly Thr Leu Thr Lys Lys Phe Gln Ile Tyr Val         515 520 525 Asp Asn Asn Thr Ser Ala Ser Gly Asp Ser Ile His Gly Gly Ser Ser     530 535 540 Arg Phe Tyr Ser Glu Thr Leu Asp Ser Leu Val Ala Gly Gln Thr Tyr 545 550 555 560 Thr Val Thr Gly Phe Thr Ala Thr Asn Ser Ser Phe Ile Thr Leu Arg                 565 570 575 Thr Glu Ser Ser Gly Gln Ile Val Leu Asp Asp Leu Ser Ile Gln Ala             580 585 590 Ala glu          <210> 91 <211> 1785 <212> DNA <213> Microbulbifer degradans <400> 91 atgaaaattt ttaaattgtt attaatgttt gtactcgccc acaacttagt tgcttgtggt 60 ggcagtaacg acggtggtga aattgaatta aactttggcg aagaaaacac accagagcca 120 gaaaccgaac cagaagctga gcctgaagga gaaccagagg gcgagccgga gggagaacct 180 gaaggagagc ctgaagggga accagagggc gaaacagccg acgcaaccgc agatgctggc 240 ttcgccggcc acaattttaa tcttaccggt ggcgaaggcg gcacagccta taccgttaat 300 aacggcaaag atttgcaaac agttttagac aacgccaaat cgagtaattc accggtcatt 360 atttacgtag acggcaccat aaattcgttt aactctgcca acggcaacca gcctattcaa 420 attaaagata tggataacgt atctataatt ggttacggcg ccgaagcaac atttgacggt 480 gttggtatag caatacgccg cgccaacaac attattattc gcaaccttac ttttaaaagc 540 gtccttaccg aaggtaaaga tgcaattagt atagaaggtg atgacgacgg cagcaccacg 600 tcaaacattt gggttgatca caacgaattc tacagcgccc caacggcaga caaagatttt 660 tacgacggtt taatcgatag taaaagcggc gcgagcaaca ttactatttc ttacaactac 720 ctgcacgacc attggaaagc atcgttacac ggccataccg aaaatgacga aggtgcacac 780 aacaccgacc gcaaaattac tttccaccac aaccgttttg agaatattga atcgcgttta 840 ccgctgttcc gtcgcggtgt aggccatttg tacaataact actacaaaga cgtaggctca 900 acggctatca actcacgtat tggtgccgag ttattaattg agaataacgt ttttgaagat 960 tcacaaaacc cgattgtctc tttttactct gacgtaattg ggtactggaa cacctcaggc 1020 aacctcttca ccaatgtaac ttggacaacc ccaggtactg gcgaagtatc tgcaggcgca 1080 acacaaacgc caacctcaga ttacgtagtg ccatacagct acacgcttat gccggcagcc 1140 gatgtaaaag cccacgtcat tgcgagtgca ggcgttggca aaatagacca gacagggctt 1200 accattccag accccgttac ccctgaaggc gacctaggtg aaccagaagc cccagtgcaa 1260 ggtgatgtaa gcctacctta cactgaaaat tttgccgcca ctgacgccgc caatttcttt 1320 agcgccgcgt accgcgatat tactggctct gctggcacca gcacacccat gtaccaccgc 1380 gtaaccggca cggtggaaat taacgcacag caattggata tgactggcgc acgcgtatca 1440 attggcaaca caacgccaag tgtaagcaca accggtgcag acaccactac aacgggcgta 1500 ttagatttaa gcgcgcccta caccgtaagc tttaaagtgg taagcgtagg cggcacccta 1560 actaagaaat ttcaaatata tgtagacaac aatacctctg ccagcggcga ctctattcac 1620 ggcggctcat cgcgctttta cagtgaaact ttagactcgc tagttgcagg ccaaacctac 1680 acagtaaccg gctttaccgc caccaacagc tctttcataa cattacgtac cgaaagtagc 1740 ggccaaattg tattagatga cctaagtatt caagccgcag aataa 1785 <210> 92 <211> 425 <212> PRT <213> Microbulbifer degradans <400> 92 Met Phe Lys Tyr Ala Leu Tyr Val Val Ala Leu Val Ala Gly Val Val 1 5 10 15 Val Ser Leu Ala Ala Cys Ser Lys Arg Ala Thr Gln Gln Val Glu Thr             20 25 30 Glu Phe Tyr Glu Ile Asn Glu Arg Gly Gly Asp Asp Gly Arg Leu Leu         35 40 45 Arg Val Val Asn Leu Asn Asn Gln Gly Val Gly Ser Leu Arg Trp Ala     50 55 60 Leu Ala Gln Thr Gly Ala Arg Lys Ile Ile Phe Asp Val Gly Gly Val 65 70 75 80 Ile Asp Leu Glu Glu Lys Ser Leu Lys Ile Arg Glu Ala His Val Thr                 85 90 95 Ile Ala Gly Glu Thr Ala Pro Ser Pro Gly Ile Thr Leu Ile Lys Gly             100 105 110 Gly Leu Arg Ile Glu Thr His Asn Val Lys Val Ser His Leu Met Ile         115 120 125 Arg Pro Gly Asp Ala Gly Tyr Ser Lys Gly Gln Gly Trp Lys Pro Asp     130 135 140 Gly Ile Thr Ile Tyr Gly Ser Lys Ala Arg His Val Val Ile Asp His 145 150 155 160 Cys Ser Val Thr Trp Ala Val Asp Glu Asn Ile Ala Val Ser Gly Pro                 165 170 175 Ala Asp Lys Gly Ala Glu Ala Thr Ala Gly Lys Val Leu Ile Arg Asn             180 185 190 Ser Ile Ile Ala Glu Ala Leu Ser Asn Ala Ser His Pro Glu Gly Glu         195 200 205 His Ser Lys Gly Ile Leu Ile His Asn Asn Val Gln His Val Ser Leu     210 215 220 Val Asn Asn Leu Leu Ala His Asn Arg Arg Arg Asn Pro Tyr Phe Lys 225 230 235 240 Ala Gly Thr Thr Gly Ile Val Ile Gly Asn Ile Ile Tyr Asn Pro Gly                 245 250 255 Lys Arg Ala Ile His Met Ser Ser Gly Arg Ala Asp Ala Ser Leu Pro             260 265 270 Thr Leu Ser Ile Thr Gly Asn Leu Phe Ile Pro Ala Ala Asn Thr Ser         275 280 285 Pro Asn Leu Ser Leu Ile Ser Asn Tyr Gly Lys Ile Tyr Ser Ser Gly     290 295 300 Asn Leu Val Gln Gly Glu Ser Arg Pro Ile Thr Asp Gly Lys Ser Ile 305 310 315 320 Ser Leu Thr Ala Pro Pro Leu Gln Gln Val Gly Ile Asn Leu Thr Asp                 325 330 335 Thr Gly Thr Gln Asn Asp Phe Cys Gln Thr Leu Ser Asn Ala Gly Ala             340 345 350 Arg Pro Trp Asp Pro Asp Pro Ile Asp Ile Arg Ile Lys Thr Gln Leu         355 360 365 Leu Ala Gly Glu Gly Arg Ile Ile Asp Ser Gln Ser Glu Val Gly Gly     370 375 380 Tyr Pro Ile His Asn Val Asn Asn Lys Glu Thr Ala Glu Ala Gly Ser 385 390 395 400 Thr Gln Gly Asp Gly Met Leu Gln Phe Asp Thr Glu Leu Leu Arg Lys                 405 410 415 Ile Pro Asn Leu Cys Ser Gly Met Met             420 425 <210> 93 <211> 1278 <212> DNA <213> Microbulbifer degradans <400> 93 atgtttaagt atgcgctata tgttgtggcc ttggtggctg gcgtagtggt tagcttagca 60 gcctgcagca agagagctac gcagcaagta gagactgaat tctacgagat taatgagcgg 120 ggcggagatg atggtcgcct gctacgcgtt gtgaatttaa ataatcaggg ggttggctct 180 ttgcgctggg cgttggcgca gacaggtgct agaaaaataa ttttcgatgt agggggggtg 240 atagatctag aagaaaaatc gctcaagatt cgtgaagccc atgtgacaat cgctggagag 300 acggccccat caccgggtat cacccttatc aagggcggac taagaataga aacccacaat 360 gtcaaagttt cgcaccttat gattaggcct ggtgacgcag ggtactccaa aggtcaaggt 420 tggaaacccg acggcataac tatatatggc agcaaagcga ggcatgttgt tattgatcat 480 tgctcggtta catgggctgt cgacgaaaat atcgcagtat ctggcccagc agataagggg 540 gcagaggcta ccgcgggtaa ggttcttatt cgcaattcaa ttattgccga agcgctaagc 600 aatgcatccc acccagaggg ggagcattct aagggcatac tcatacacaa caatgtgcag 660 catgtaagct tggttaacaa tttgttggct cacaataggc gaagaaaccc ttattttaag 720 gcgggtacaa cgggaattgt aattggcaat ataatttata acccagggaa acgtgctatt 780 catatgtctt cgggccgtgc tgatgctagc ctgccaacac tgtcaattac agggaatttg 840 tttattccag cagctaatac ttcccccaac cttagtttga taagcaacta tggaaaaatt 900 tattcgagtg gaaacttggt gcagggggag agcaggccga taactgatgg taaaagtatt 960 tcattgactg cgccgccgct acagcaagta ggcataaatt taaccgatac aggtacacaa 1020 aacgactttt gccaaaccct aagcaacgca ggtgcccgcc cgtgggaccc cgacccgatt 1080 gatattagaa taaaaacaca acttttggct ggcgaaggac gaattattga tagccagagc 1140 gaggttggag gctacccaat acataacgtc aataacaaag aaaccgctga agcaggctct 1200 acgcaggggg atggtatgtt gcagtttgat actgagttat tgagaaaaat accaaacctg 1260 tgtagtggca tgatgtaa 1278 <210> 94 <211> 772 <212> PRT <213> Microbulbifer degradans <400> 94 Met Arg Asp Ile Thr Met Lys Asn Asn Lys Phe Arg Ser Ser Phe Thr 1 5 10 15 Leu Lys Lys Leu Thr Pro Phe Phe Val Ala Gly Thr Met Leu Gly Gly             20 25 30 Ser Asn Ala Trp Ala Gly Cys Asp Tyr Thr Val Thr Asn Gln Trp Gly         35 40 45 Ser Gly Phe Thr Gly Asn Val Arg Ile Thr Asn Ser Gly Asn Thr Pro     50 55 60 Thr Asn Gly Trp Ala Val Asn Trp Gln Tyr Ala Gly Asp Asn Arg Ile 65 70 75 80 Ser Asn Ser Trp Gly Ala Gln Leu Ser Gly Ser Asn Pro Tyr Ser Ala                 85 90 95 Thr Ala Glu Ser Trp Asn Ala Val Ile Gln Pro Ser Gln Ser Ile Glu             100 105 110 Ile Gly Phe Gln Gly Thr Gly Asp Gly Asn Glu Ile Pro Thr Ile Asn         115 120 125 Gly Asp Val Cys Gln Thr Ser Ser Gly Ser Thr Ser Ser Ser Ser Ser     130 135 140 Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser 145 150 155 160 Ser Asn Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser                 165 170 175 Ser Gly Ser Thr Thr Gly Tyr Ile His Ile Glu Glu Asn Glu Leu Gly             180 185 190 Phe Cys Tyr Val Gln Gly Ser Ile Asp Ser Asn Asn Gly Phe Thr         195 200 205 Gly Thr Gly Phe Ala Asn Thr Asp Asn Val Asn Gly Ser Gln Ile Asn     210 215 220 Trp Lys Val Asn Val Asp Phe Asp Gly Tyr Tyr Ala Leu Glu Trp Arg 225 230 235 240 Tyr Ala Asn Gly Ser Gly Thr Ala Arg Thr Ala Ser Val Ser Ala Asn                 245 250 255 Gly Ala Gln Ser Glu Ile Ser Phe Pro Thr Thr Gly Ser Trp Asp Ser             260 265 270 Trp Leu Leu Asp Ser Thr Thr Leu Phe Leu Lys Ala Gly Val Asn Asp         275 280 285 Val Ile Leu Ser Ala Asn Thr Ser Ser Gly Leu Ala Asn Ile Asp Ser     290 295 300 Leu Thr Val His Gly Asp Gly Val Ala Ala Ala Asp Cys Asn Thr Asp 305 310 315 320 Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr                 325 330 335 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser             340 345 350 Gly Gly Pro Gln Ile Leu Lys Ala Phe Pro Thr Ala Glu Gly Tyr Gly         355 360 365 Lys Ile Thr Ala Gly Gly Arg Gly Gly Asp Val Tyr Ile Val Thr Asn     370 375 380 Leu Asn Asp Ser Gly Ala Gly Ser Leu Arg Gln Ala Val Glu Ala Ser 385 390 395 400 Gly Pro Arg Thr Val Val Phe Glu Val Ser Gly Thr Ile Thr Leu Asn                 405 410 415 Lys Pro Leu Thr Ile Lys Asn Asn Asn Ile Thr Ile Ala Gly Gln Thr             420 425 430 Ala Pro Gly Asp Gly Ile Thr Leu Arg Lys His Asn Phe Ser Ile Gln         435 440 445 Ala Asp Asp Val Ile Val Arg Tyr Ile Arg Val Arg Phe Gly Asp Glu     450 455 460 Thr Leu Thr Asp Ser Asp Ala Ile Ser Met Arg Tyr Gln Lys Asn Ile 465 470 475 480 Ile Leu Asp His Val Ser Ala Ser Trp Gly Asp Asp Glu Thr Leu Ser                 485 490 495 Leu Tyr His Gly Glu Asn Ile Thr Val Gln Trp Ser Met Ile Thr Glu             500 505 510 Thr Leu Asn Arg Gly Gly Glu His Ala Phe Ala Ala Ile Trp Gly Ser         515 520 525 Pro Phe Ser Thr Phe His His Asn Leu Ile Ala His Asn Val Ala Arg     530 535 540 Asn Val Arg Phe Ala Ser Gly Ser Gly Tyr Thr Asp Tyr Arg Asn Asn 545 550 555 560 Val Val Tyr Asn Trp Gly Tyr Ser Ser Thr His Gly Gly Glu Ala Gln                 565 570 575 Gln Val Gly Asn Ala Asn Phe Asn Phe Thr Thr Val Asn Met Val Gly             580 585 590 Asn Tyr Tyr Lys Pro Gly Pro Arg Thr Glu Ser Gly Val Arg Ser Arg         595 600 605 Leu Leu Thr Pro Asn Thr Arg Asn Gly Asp Ala Asp Leu Gly Ser Phe     610 615 620 Tyr Val Ser Gly Asn His Met Val Gly Ser Pro Asn Val Thr Ala Asp 625 630 635 640 Asn Ser Ile Gly Val Ser Asn Lys Asn Ala Leu Ile Ser Ser Pro Trp                 645 650 655 Asn Ser Met Lys Ile Glu Gly Glu Gln Thr Ala Glu Gln Ala Tyr Glu             660 665 670 Ser Val Leu Ala Tyr Ala Gly Ala Ser Lys Val Arg Asp Ser Val Asp         675 680 685 Thr Arg Ile Ile Glu Glu Val Arg Thr Gly Thr Ala Thr Tyr Gly Gly     690 695 700 Asn Gly Ile Ile Glu Ser Gln Asn Glu Val Gly Gly Trp Pro Gln Leu 705 710 715 720 Arg Ser Glu Thr Pro Pro Gln Asp Ser Asp Arg Asp Gly Met Pro Asp                 725 730 735 Asp Trp Glu Arg Ala Asn Asn Leu Asn Pro Phe Asn Ala Ala Asp Arg             740 745 750 Asn Thr Lys Asp Ser Ile Gly Tyr Thr Met Leu Glu Arg Tyr Ile Asn         755 760 765 Gly leu val asp     770 <210> 95 <211> 2319 <212> DNA <213> Microbulbifer degradans <400> 95 atgagagata tcacgatgaa gaataataaa ttcaggtcgt cttttacatt aaaaaaactc 60 acaccgtttt ttgttgcggg caccatgctt ggcggttcca acgcctgggc tggctgcgac 120 tatacggtca ctaatcagtg gggctcaggc tttactggca acgttcgtat aactaatagc 180 ggtaacacgc caacaaatgg ttgggctgtt aactggcagt acgctggcga taatcgtatt 240 agtaatagct ggggagcaca gctttcaggg tcgaacccat actctgccac ggcagaaagc 300 tggaatgctg ttattcagcc tagtcagtcc atagaaattg ggtttcaagg taccggcgac 360 ggaaatgaaa taccaactat aaatggcgat gtttgccaga ctagcagcgg aagtacttca 420 tccagctcat cttcaagtac gtcttctagc agctcttcaa gctcgtccac tagcagctct 480 tcaaacagct cttctagctc tagctcgtcc agctcgtcta gctcctcctc tggctctaca 540 actggatata ttcatataga agagaatgaa cttggttttt gttatgtaca aggttccatt 600 gactccaaca acggtggctt taccggcaca ggctttgcca ataccgataa cgttaatggc 660 tcacagatta actggaaagt aaatgtcgac tttgatggat attatgcgct cgaatggcgc 720 tatgcgaatg gctccggcac cgcgcgcact gcaagcgtta gcgctaatgg agcacaaagc 780 gaaatttcct tccctacaac aggttcgtgg gatagctggt tattagacag cactacccta 840 tttttaaaag ctggcgtaaa cgacgtaata ttgagcgcaa atacaagcag tggcctagcg 900 aacatagatt cacttacagt gcacggtgat ggcgtagctg cggcagactg taatactgat 960 ggaagctcaa gcagcagctc tagttcaagc tctagttcca gctcaacttc tagtagctcc 1020 tcaagttcca gctcgtctag cacgtccagc tcttctggtg gcccgcaaat attaaaagca 1080 ttccccaccg cagaaggcta cggaaaaata accgcaggtg gtcgtggtgg cgatgtctat 1140 atagttacaa acctgaatga ctcaggcgcg ggtagtttgc gtcaggccgt agaggcatct 1200 ggccctagaa ccgttgtgtt cgaagtgtct ggaaccatca ctctaaataa accactcaca 1260 atcaaaaata ataacatcac aatagcagga caaactgcac caggcgatgg cattacactt 1320 agaaagcaca acttttctat ccaagctgat gatgtcatcg tacgttacat acgtgttcgc 1380 tttggtgatg aaaccctaac cgattctgat gcgatttcca tgaggtacca aaaaaatatt 1440 attttggatc atgtgagtgc tagctgggga gatgatgaaa ccttatctct ttatcacggc 1500 gaaaatatca ctgtgcaatg gagcatgatt acagagaccc tcaatcgtgg cggcgaacat 1560 gcattcgcag ctatatgggg ttcgcctttt agtaccttcc accacaattt aattgctcac 1620 aatgttgcga gaaacgttcg ctttgcgtcg ggttccggtt atacggatta tcgtaacaat 1680 gtcgtatata actggggcta tagcagcaca cacggaggcg aagctcaaca agttggcaac 1740 gctaatttta atttcaccac cgtcaatatg gtcggcaact attacaaacc tgggccgaga 1800 actgaatctg gcgttcgtag tcgactactt acacctaaca cgcgtaacgg cgatgcggac 1860 ttaggtagtt tttacgtttc tggtaaccac atggttggca gcccaaatgt aactgcagac 1920 aactcgattg gcgtatcgaa taaaaatgcc ttaataagta gcccttggaa ttcaatgaaa 1980 atagaaggcg aacaaacagc tgagcaagca tatgagtcag ttcttgctta cgcaggtgca 2040 tctaaagtac gcgactcggt agatactcgt attattgaag aagtacgtac aggcacagct 2100 acttatggtg gaaacggcat aattgaatcg cagaatgaag tgggtggttg gccacaactt 2160 agaagtgaaa cgcccccgca agacagtgat cgcgacggaa tgccagatga ctgggaacgc 2220 gcgaacaacc taaatccatt caacgcagcc gatagaaaca ctaaagacag tattggctac 2280 acaatgttag agcgatatat taacgggctt gttgattaa 2319 <210> 96 <211> 511 <212> PRT <213> Microbulbifer degradans <400> 96 Met Leu Arg Ile Pro Lys Ala Trp Leu Ala Leu Pro Leu Val Leu Gly 1 5 10 15 Ser Thr Asn Leu Tyr Ala Gln Val Thr Cys Ser Ile Ser Asn Thr Asn             20 25 30 Val Trp Asn Asn Gly Tyr Thr Val Asn Val Asn Val Thr Asn Thr Gly         35 40 45 Ser Ser Gln Val Gly Ser Trp Gln Val Pro Ile Asn Phe Ser Glu Pro     50 55 60 Pro Gln Val Ser Ser Gly Trp Asn Ala Ile Leu Ser Thr Asn Gly Asn 65 70 75 80 Thr Val Thr Ala Gly Asn Ile Gly Trp Asn Gly Asn Leu Asn Pro Gly                 85 90 95 Gln Ser Ala Ser Phe Gly Phe Gln Gly Gly His Asp Gly Ser Phe Val             100 105 110 Glu Pro Thr Cys Ser Gly Gly Gly Ser Ser Thr Ser Ser Ser Ser Ser         115 120 125 Ser Ser Ser Ser Ser Thr Ser Ser Thr Ser Ser Ser Ser Thr Ser Ser     130 135 140 Ser Ser Ser Ser Ser Ser Gly Gly Ser Glu Leu Leu Ile Gln Glu Asn 145 150 155 160 Ala Ser Gly Phe Cys Arg Val Asp Gly Ser Ile Asp Asn Asn Asn Ser                 165 170 175 Gly Tyr Thr Gly Ser Gly Phe Ala Asn Thr Glu Asn Gln Asn Gly Ser             180 185 190 Ala Val Glu Tyr Ala Leu Asn Val Pro Ser Asn Gly Asn Tyr Leu Leu         195 200 205 Asp Ala Arg Tyr Ala Ser Ala Thr Thr Arg Ser Ala Ser Val Val Val     210 215 220 Asn Gly Ser Ser Val Gly Ser Phe Ser Phe Pro Ser Thr Gly Ser Trp 225 230 235 240 Thr Ser Trp Thr Val Asp Ser Ala Asn Val Pro Leu Lys Gly Gly Asn                 245 250 255 Asn Ile Val Arg Ile Val Ala Thr Asn Ser Ser Gly Leu Pro Asn Ile             260 265 270 Asp Ser Leu Lys Val Ile Gly Thr Asn Pro Ser Ala Gly Ser Cys Ser         275 280 285 Ser Asn Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser     290 295 300 Ser Asn Ser Gly Gly Lys Gly Ser Ser Cys Arg Ser Thr Gly Ser Gln 305 310 315 320 Ser Val Ser Ser Thr Ile Lys Val Thr Ser Gly Thr Phe Asp Gly Asn                 325 330 335 Cys Lys Thr Tyr Asn Pro Thr Ser Ala Leu Gly Asp Gly Ser Gln Ser             340 345 350 Glu Ser Gln Lys Pro Ala Phe Arg Val Glu Asn Gly Ala Thr Leu Lys         355 360 365 Asn Val Ile Leu Gly Asn Asn Gly Val Asp Gly Ile His Val Tyr Asn     370 375 380 Gly Gly Thr Leu Asp Asn Ile Arg Trp Thr Asn Val Gly Glu Asp Ala 385 390 395 400 Met Thr Val Lys Ser Glu Gly Asn Val Thr Val Ser Asn Ile Glu Gly                 405 410 415 Tyr Asp Gly Ser Asp Lys Phe Ile Gln Val Asn Ala Val Thr Asn Leu             420 425 430 Lys Val Ser Asn Cys Ile Val Asp Lys Met Gly Lys Phe Leu Arg Gln         435 440 445 Asn Gly Gly Lys Thr Phe Ala Met Ser Val Thr Val Asp Asn Cys Asp     450 455 460 Ile Ser Asn Met Gly Glu Gly Val Phe Arg Ser Asp Ser Pro Asn Ala 465 470 475 480 Thr Ala Arg Ile Thr Asn Ser Arg Leu Lys Asn Ala Gly Asp Ile Cys                 485 490 495 Ile Gly Lys Trp Lys Ser Cys Thr Ser Ser Asn Ile Thr Ser Phe             500 505 510 <210> 97 <211> 1536 <212> DNA <213> Microbulbifer degradans <400> 97 atgttgcgaa tccccaaggc ttggctggca cttccacttg tactgggaag taccaatcta 60 tacgctcaag taacttgcag tatctctaac accaatgttt ggaataacgg atacaccgtt 120 aatgttaatg taaccaacac aggctcttca caggttggtt cttggcaggt tcctattaat 180 ttttctgagc cacctcaagt aagcagcggc tggaatgcaa tattaagcac aaacggaaac 240 accgtaactg ccggcaatat tggttggaat ggtaatttaa atcccggcca aagcgcctcc 300 tttggttttc aaggtggcca cgatggcagc tttgtggagc ccacctgctc gggcggaggc 360 tctagcacta gctcaagcag ctctagtagt tctagctcaa caagttctac cagttcttca 420 tccacaagtt caagtagctc ttctagctcc ggcggctctg aacttttaat ccaagaaaat 480 gcatccggct tctgccgtgt ggacggatcg atagataaca ataactcagg ctataccggt 540 agtggctttg ccaacaccga gaaccaaaac ggttccgcag ttgaatacgc acttaacgtt 600 ccctctaatg ggaattatct cctcgacgct cgatatgcaa gcgctactac acgatcggct 660 agcgtggtag ttaatggatc ttcagtaggc agctttagtt ttccatctac gggttcgtgg 720 acaagctgga cagttgactc cgccaacgtt ccgttaaaag gcgggaataa tattgttcga 780 attgttgcaa ctaacagcag cggattacct aatattgatt cattaaaggt aataggcacc 840 aacccgtcag ccggcagttg ttcaagcaac tcgtcatcca ctagttcatc gtctagctca 900 agttcatcaa gcagtaactc cggtggcaaa ggctctagct gccgttctac aggcagtcaa 960 tctgtttcct ctactattaa agttactagc gggactttcg atgggaactg taaaacgtat 1020 aaccctacaa gtgcccttgg cgatggcagt caatcagaaa gccagaaacc ggcattccga 1080 gtggagaacg gcgcaacact caaaaacgtg attctaggca acaatggcgt agacggtatt 1140 catgtttata acggcggcac cttggataac atccgctgga ccaatgtggg tgaagatgca 1200 atgaccgtta aatctgaagg aaacgttacc gtttcaaata ttgagggtta tgacggttca 1260 gataaattta tacaagtaaa cgcagttacc aacctaaagg tttctaattg cattgtagat 1320 aaaatgggta aatttttacg tcagaatggc ggtaaaactt tcgctatgtc tgtaaccgta 1380 gataattgtg atatctcaaa tatgggtgaa ggtgttttcc gctcagacag cccaaatgca 1440 acagcgagaa tcacaaatag ccgattaaaa aatgcaggcg acatttgtat tggtaagtgg 1500 aaaagctgca catcttccaa cattaccagc ttctaa 1536 <210> 98 <211> 455 <212> PRT <213> Microbulbifer degradans <400> 98 Met Ile Met Met Arg Asn Lys Ile Leu Leu Ala Leu Val Leu Cys Gly 1 5 10 15 Ala Ser Ala Ser Ala Phe Ala Ala Ser Asn Arg Pro Ser Gly Tyr Thr             20 25 30 Thr Ile Cys Lys Thr Asp Gln Thr Cys Ser Val Ser Ser Ser Thr Asn         35 40 45 Val Ala Phe Gly Ala Ala Gly Lys Phe Val Tyr Lys Val Ile Asn Gly     50 55 60 Thr Phe Thr Cys Asn Thr Ser Thr Phe Gly Ser Asp Pro Asn Pro Ala 65 70 75 80 Lys Ser Val Lys Glu Cys Ser Val Pro Thr Asn Gly Ser Ser Ser Thr                 85 90 95 Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly             100 105 110 Ser Ser Ser Ser Cys Gly Thr Gly Gly Gly Ala Thr Val Cys Leu Ser         115 120 125 Ala Gly Gly Gly Ser Asn Asp Ile Asp Leu Thr Trp Thr Val Ser Gly     130 135 140 Ser Ile Ser Ser Ala Gln Val Tyr Arg Asp Thr Asp Ser Asn Pro Ser 145 150 155 160 Gly Arg Thr Arg Ile Ala Gln Leu Gly Gly Asp Ala Arg Ser Tyr Ser                 165 170 175 Asp Thr Asn Val Ser Ala Gly Lys Gln Tyr Tyr Tyr Trp Ile Lys Phe             180 185 190 Gly Ala Asn Gly Ser Asn Tyr Asn Ser Asn Ala Ala Ser Ala Thr Tyr         195 200 205 Ser Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser     210 215 220 Ser Gly Gly Ser Ala Glu Cys Lys Ala Gly Ala Thr Ile Ser Gly Lys 225 230 235 240 Thr Val Asp Cys Gly Gly Lys Glu Ile Gly Leu Ser Cys Ser Gly Asp                 245 250 255 Ser Glu Thr Gln Pro Pro Val Leu Thr Leu Lys Asn Ala Thr Ile Lys             260 265 270 Asn Leu Val Ile Ser Ala Lys Gly Gly Ser Asp Gly Ile His Cys Thr         275 280 285 Gly Asn Cys Thr Met Glu Asn Val Val Trp Lys Asp Ile Cys Glu Asp     290 295 300 Ala Ala Thr Asn Lys Thr Asp Gly Ile Thr Met Thr Ile Ile Gly Gly 305 310 315 320 Ser Ala Tyr Asn Ser Thr Ser Gly Tyr Gly Gly Lys Pro Asp Lys Val                 325 330 335 Phe Gln His Asn Ser Lys Asn Ser Thr Thr Val Ile Lys Gly Gly Phe             340 345 350 Thr Leu Thr Gly Glu His Gly Lys Leu Trp Arg Ser Cys Gly Asn Cys         355 360 365 Thr Asn Asn Gly Gly Pro Arg Asn Val Thr Ile Asp Asn Val Lys Val     370 375 380 Asp Ala Lys Ile Gly Ser Ile Val Gly Val Asn Arg Asn Tyr Gly Asp 385 390 395 400 Lys Ala Thr Ile Lys Asn Leu Lys Ile Lys Asp Tyr Lys Ser Gly Ser                 405 410 415 Pro Lys Val Cys Glu Glu Tyr Lys Gly Val Gln Lys Gly Ser Gly Glu             420 425 430 Ser Ser Lys Tyr Gly Glu Tyr Trp Asp Thr Ala Asn Cys Asp Val Ser         435 440 445 Lys Ser Asp Val Ser Ala Leu     450 455 <210> 99 <211> 60 <212> PRT <213> Microbulbifer degradans <400> 99 Met Pro His Glu Leu Leu Glu Pro Asp Glu Leu Leu Glu Leu Glu Leu 1 5 10 15 Glu Glu Leu Leu Val Leu Leu Val Leu Glu Glu Pro Leu Val Gly Thr             20 25 30 Glu His Ser Phe Thr Asp Leu Ala Gly Leu Gly Ser Leu Pro Lys Val         35 40 45 Asp Val Leu Gln Val Lys Val Pro Leu Ile Thr Leu     50 55 60 <210> 100 <211> 1368 <212> DNA <213> Microbulbifer degradans <400> 100 gtgataatga tgcgtaataa aatcctattg gcgcttgtat tgtgtggagc ttctgcctct 60 gcctttgcgg ctagtaatcg tcctagtggt tacacaacta tctgtaaaac cgatcaaact 120 tgttctgtaa gctcgtctac caacgttgcg ttcggcgctg ctggtaagtt tgtttacaaa 180 gtaattaacg gtacctttac ttgtaataca tctacttttg gcagcgatcc taaccctgct 240 aaatctgtaa aagaatgttc tgtacctact aatggttctt ctagcactag cagcaccagt 300 agttcttcta gctctagttc aagtagctca tcgggttcaa gcagctcatg tggcactggt 360 ggcggcgcaa ctgtatgttt aagtgcaggt ggcggcagta acgatatcga tttaacttgg 420 acagtatctg gttctatttc tagcgctcag gtttaccgcg acacagattc taaccctagt 480 ggtcgcacac gtattgctca attaggtggc gatgcaagaa gctatagcga tacgaatgtt 540 agtgctggta agcagtacta ctactggatt aagtttggcg ccaacggctc taactacaat 600 tcgaatgcgg cttctgctac ctatagtggt tcaagtagct catcgagttc ttcaagctct 660 tctagttcct catctggcgg ctcggctgaa tgtaaagctg gcgctactat ttctggtaaa 720 accgtagatt gcggtggtaa agaaattggc ttgtcgtgct cgggtgatag tgaaactcaa 780 ccaccagtat taacgcttaa aaatgccacc attaaaaact tggtaatttc tgctaaaggt 840 gggtccgacg gtattcactg tactggcaac tgcaccatgg aaaatgttgt ttggaaagat 900 atctgtgaag atgctgctac caacaaaacc gacggtatta ccatgaccat tattggtggt 960 agtgcgtata actctacaag cggttacggc ggcaagccag ataaggtttt ccaacataac 1020 tctaaaaaca gtactactgt aattaaaggc ggctttacat taacaggtga gcacggcaaa 1080 ttgtggcgtt catgtggtaa ctgtactaat aacggcggcc cacgtaatgt gactatcgac 1140 aacgttaaag tagacgcgaa aataggcagt attgttggcg ttaaccgcaa ctatggcgat 1200 aaggcaacaa tcaaaaactt aaagattaaa gactacaaat ctggtagccc caaagtgtgt 1260 gaagaataca agggtgtaca aaagggtagt ggcgagtctt ctaagtatgg cgaatactgg 1320 gatactgcaa actgcgatgt aagtaaatca gatgtgtctg ctctttaa 1368 <210> 101 <211> 424 <212> PRT <213> Microbulbifer degradans <400> 101 Met Phe Asn Lys Ile Leu Val Ala Val Gly Leu Leu Ala Ala Ser Leu 1 5 10 15 Ser Val His Ala Ala Thr Asn Arg Pro Ser Gly Tyr Thr Thr Ile Cys             20 25 30 Lys Val Gly Glu Thr Cys Ser Val Ser Gln Ser Thr Asn Val Ala Phe         35 40 45 Gly Ala Ser Gly Gln Phe Val Tyr Lys Val Leu Asn Gly Ser Phe Ser     50 55 60 Cys Ser Val Ser Thr Phe Gly Ser Asp Pro Ile Pro Ser Lys Ser Val 65 70 75 80 Lys Glu Cys Ser Ile Pro Ser Asn Gly Ser Ser Ser Ser Gly Ser Ser                 85 90 95 Ser Ser Ser Ser Ser Ser Ser Ser Gly Ser Ser Ser Gly Gly Gly Cys             100 105 110 Gly Ser Gly Gly Gly Ser Thr Val Cys Leu Ser Ala Ser Gly Ser Ser         115 120 125 Asn Gly Ile Asn Leu Ser Trp Ser Val Ser Gly Ser Ile Ser Ser Val     130 135 140 Gln Leu Tyr Arg Asp Thr Asp Ser Asn Pro Ser Gly Arg Thr Arg Ile 145 150 155 160 Ala Ser Val Ser Ser Ser Thr Thr Ser Phe Ser Asp Thr Gly Ala Ala                 165 170 175 Ser Gly Thr Thr Tyr Tyr Tyr Trp Val Lys Tyr Tyr Val Asn Gly Thr             180 185 190 Ala Tyr Asn Ser Gly Val Ala Ser Ala Val Arg Gly Ser Ser Ser Ser         195 200 205 Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Gly Gly Lys Gly     210 215 220 Ser Ser Cys Ser Ser Thr Gly Ser Gln Ser Val Ser Ser Thr Ile Lys 225 230 235 240 Val Thr Ser Gly Thr Tyr Asp Gly Gly Cys Lys Thr Phe Asn Pro Thr                 245 250 255 Ser Ala Leu Gly Asp Gly Ser Gln Ser Glu Ser Gln Lys Pro Ala Phe             260 265 270 Arg Val Glu Asn Gly Ala Thr Leu Lys Asn Val Ile Ile Gly Asn Asn         275 280 285 Gly Val Asp Gly Ile His Val Tyr Asn Gly Gly Thr Leu Asn Asn Ile     290 295 300 Leu Trp Thr Asn Val Gly Glu Asp Ala Met Thr Val Lys Ser Glu Gly 305 310 315 320 Asn Val Thr Val Thr Asn Val Glu Gly Tyr Asp Gly Glu Asp Lys Phe                 325 330 335 Ile Gln Val Asn Ala Val Thr Asn Leu Lys Val Ser Asn Cys Ile Val             340 345 350 Asn Lys Met Gly Lys Phe Leu Arg Gln Asn Gly Gly Lys Thr Phe Ala         355 360 365 Met Ser Val Ser Val Asp Asn Cys Asp Ile Ser Asn Met Gly Glu Gly     370 375 380 Ile Phe Arg Ser Asp Ser Pro Asn Ala Thr Ala Val Ile Thr Asn Ser 385 390 395 400 Arg Leu Arg Asn Ala Gly Asp Ile Cys Ile Gly Ala Trp Lys Ser Cys                 405 410 415 Lys Ser Ser Asn Ile Ser Ser Phe             420 <210> 102 <211> 1275 <212> DNA <213> Microbulbifer degradans <400> 102 atgtttaaca agatactcgt tgcagtagga ttacttgcgg ctagcctttc tgtgcacgcc 60 gcaacaaacc gcccaagtgg ttatacaaca atttgtaagg ttggtgaaac atgctcggta 120 agtcagtcta cgaatgtagc ctttggcgcg tctgggcagt ttgtgtataa agtattaaac 180 ggtagctttt cttgtagtgt ttctacgttt ggtagtgacc ctattccttc taaatctgta 240 aaagaatgtt caatcccatc aaacggctct agctcttctg gctcgtcttc atcttcgtct 300 agcagctctt ccggtagctc ttctggtggt ggctgtggca gcggtggtgg ttctacggtg 360 tgcttatcgg cctcgggttc tagcaatggt atcaatttaa gttggtctgt atctggttct 420 atatcttccg tgcagcttta tcgcgatacc gattcaaacc caagcggtcg cacgcgtatt 480 gctagtgtat ctagctctac tactagcttt agtgataccg gcgcggcatc gggcaccact 540 tattactact gggttaaata ttatgtaaat ggtactgctt acaactcggg tgttgcttct 600 gcggtgcgcg gttcttctag ctctagtagt tcaagttctt ccagcacttc tagcagttct 660 ggtggaaaag gttctagttg tagctctact ggtagccaat ctgtgtcttc tactattaag 720 gtaaccagcg gtacttacga tggtggttgt aaaacattta accctaccag tgctttgggt 780 gatggtagcc aatctgaaag ccaaaaacct gctttccgtg tagaaaacgg tgcaacgtta 840 aagaatgtaa ttattggcaa taacggtgtg gatggtattc acgtttacaa cggcggtacg 900 ttaaataata ttctttggac taacgtaggt gaagatgcca tgaccgttaa gtctgaaggt 960 aacgtgacgg taaccaatgt tgaaggctat gacggcgaag ataagtttat tcaggtaaac 1020 gcagtgacta acttaaaagt ttctaactgt attgtgaata aaatgggtaa gtttcttcgt 1080 cagaatggtg gtaaaacatt tgccatgtcg gtaagtgtag ataactgcga tatatctaat 1140 atgggtgaag gtatcttccg ttcagacagc ccgaacgcta cagcggttat tactaacagc 1200 cgtttacgca acgctgggga tatttgtatt ggggcttgga aaagttgtaa atcttccaat 1260 atcagcagct tttaa 1275 <210> 103 <211> 392 <212> PRT <213> Microbulbifer degradans <400> 103 Met Lys Lys Leu Ile Leu Met Val Ala Leu Leu Ala Phe Ser Val Ser 1 5 10 15 Ser Phe Ala Ala Leu Ser Ser Gly Arg Tyr Ile Ile Val Ser Lys Leu             20 25 30 Asn Gly Asn Ala Leu Asp Val Asp Ser Phe Ser Thr Ala Asp Gly Ala         35 40 45 Asn Val Met Gln Trp Phe Ala Leu Gly Gly Val Asn Gln Gln Phe Asp     50 55 60 Val Ala Val Leu Ser Asp Gly Ser Tyr Ser Ile Arg Pro Val His Ser 65 70 75 80 Gly Lys Ser Leu Asp Val Tyr Ala Trp Asn Ala Asp Asp Gly Ala Glu                 85 90 95 Leu Arg Gln Trp Ala Tyr Thr Gly Ala Asp Asn Gln Arg Trp Tyr Ile             100 105 110 Asp Asn Gln Ser Gly Asp Tyr Tyr Ser Ile Thr Ser Lys Phe Ser Gly         115 120 125 Arg Ala Leu Asp Val Trp Gly Met Ser Met Tyr Thr Gly Ala Asp Val     130 135 140 Arg Leu Tyr Ser Tyr Trp Gly Gly Ala Gly Gln Leu Trp Thr Phe Gln 145 150 155 160 Lys Val Gly Ser Ser Ser Glu Cys Tyr Ala Gly Ala Thr Leu Thr Asn                 165 170 175 Arg Phe Val Asp Cys Gly Gly Lys Thr Ile Gly Leu Ser Cys Val Gly             180 185 190 Asp Ser Glu Thr Gln Gly Ala Val Leu Thr Leu Lys Asn Ser Ser Ile         195 200 205 Arg Asn Val Lys Leu Ala Ala Asn Gly Gly Ala Asp Gly Ile His Cys     210 215 220 Thr Ser Gly Asn Cys Thr Leu Ala Asp Val Val Trp Asn Asp Ile Cys 225 230 235 240 Glu Asp Ala Ala Thr Asn Lys Ser Glu Gly Gly Thr Leu Thr Ile Val                 245 250 255 Gly Gly Ser Ala Tyr Asn Ser Thr Gly Gly Tyr Gly Gly Thr Pro Asp             260 265 270 Lys Ile Phe Gln His Asn Ser Lys Asn Ser Thr Thr Ile Val Ala Gly         275 280 285 Gly Phe Thr Ala Tyr Gly Thr His Gly Lys Leu Trp Arg Ser Cys Gly     290 295 300 Asn Cys Thr Asn Asn Gly Gly Pro Arg Asn Leu Leu Val Tyr Ser Val 305 310 315 320 Asn Ile Asp Ala Ser Ile Gly Ala Ile Ala Gly Val Asn Arg Asn Tyr                 325 330 335 Gly Asp Arg Ala Thr Ile Arg Asp Leu Lys Ile Lys Asn Tyr Ser Ser             340 345 350 Gly Ser Pro His Val Cys Asp Glu Tyr Gln Gly Val Gln Lys Gly Asn         355 360 365 Ser Ser Thr Lys Tyr Gly Glu Tyr Trp Asn Thr Ala Ser Cys Asp Val     370 375 380 Ser Arg Ser Asp Val Ser Gly Leu 385 390 <210> 104 <211> 1179 <212> DNA <213> Microbulbifer degradans <400> 104 atgaaaaaac ttatccttat ggtggcgctg ttggctttta gtgttagttc ttttgctgca 60 ctgtcttcag gccgctacat tattgtttct aaacttaatg gcaacgcgtt agatgtagat 120 agctttagca ccgcagatgg cgccaatgtt atgcagtggt ttgctttggg tggtgtgaac 180 cagcagtttg acgtggcagt gcttagcgat ggcagttact ccatacgacc agtgcacagc 240 ggtaagtcat tagatgtata tgcgtggaac gcagacgatg gtgcggaact tcgtcagtgg 300 gcatacacag gcgcagataa ccaacgttgg tatatcgata atcaaagtgg cgattactat 360 tcaattacgt ctaaatttag cgggcgcgca ttggatgtat ggggtatgag tatgtacacc 420 ggcgcagatg tccgccttta ttcatattgg ggcggcgcgg ggcagctgtg gaccttccaa 480 aaggtaggta gctcaagtga gtgttacgca ggtgctacgt taacaaaccg ctttgtggat 540 tgtggcggca aaacaatagg ccttagttgt gtaggcgata gtgaaactca aggcgcggtg 600 ctaaccctta aaaactcgtc cattcgcaat gttaagttgg ctgcaaacgg tggtgcggat 660 ggcattcact gcactagtgg caactgcaca ttagccgacg ttgtttggaa cgatatttgt 720 gaagatgctg ccacgaataa gtctgaaggt ggcaccctga ctattgtggg tggttcggcg 780 tataactcta ctggcgggta tggtggtaca ccggataaaa tttttcagca caactcgaaa 840 aacagcacaa caattgttgc cggcggcttc actgcatatg gtacccacgg taagttgtgg 900 cgctcgtgtg gtaactgtac aaacaacggc ggtccgcgta atttactggt ttatagcgtg 960 aatattgacg caagtattgg cgcaattgct ggtgttaacc gcaattacgg cgatagagcg 1020 accattcgcg acctaaaaat aaagaattat tcttctggca gcccgcatgt gtgtgacgaa 1080 tatcaaggcg tacagaaggg caattcttct acaaaatatg gcgagtactg gaataccgca 1140 agttgtgatg tttcgcggtc agatgtaagt gggctttaa 1179 <210> 105 <211> 733 <212> PRT <213> Microbulbifer degradans <400> 105 Met Phe Arg Tyr Ile Leu Thr Ala Phe Ala Leu Val Ala Ala Ala Ser 1 5 10 15 Cys Ala Gln Ala Ala Thr Asn Arg Pro Ser Gly Tyr Thr Thr Ile Cys             20 25 30 Lys Thr Asn Gln Thr Cys Ser Val Ser Ser Pro Thr Asn Val Ala Phe         35 40 45 Gly Ala Ser Gly Lys Phe Thr Phe Lys Val Leu Asn Gly Ser Phe Val     50 55 60 Cys Ser Val Ala Thr Phe Gly Ser Asp Pro Asn Pro Ala Lys Ser Ala 65 70 75 80 Lys Glu Cys Ser Ile Pro Ser Asp Gly Ser Ser Ser Thr Ser Ser Thr                 85 90 95 Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser             100 105 110 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser         115 120 125 Ser Ser Ser Ser Ser Gly Ser Ser Gln Ala Gly Cys Gly Ser Gly Gly     130 135 140 Gly Ala Thr Val Cys Leu Ser Ala Thr Asp Thr Ala Ser Ala Ile Asn 145 150 155 160 Leu Asn Trp Thr Val Ser Gly Ser Leu Ser Ser Val Gln Val Tyr Arg                 165 170 175 Asp Thr Asp Pro Asn Pro Ser Gly Arg Thr Arg Leu Thr Ser Leu Ser             180 185 190 Pro Ser Val Thr Ser Tyr Thr Asp Asn Asn Ala Gln Ala Gly Thr Thr         195 200 205 Tyr Tyr Tyr Trp Ile Lys Phe Gly Ala Asn Gly Ser Asn Tyr Asn Ser     210 215 220 Gly Ala Ala Ser Ala Val Ile Ala Asn Thr Gly Asn Asp Asp Glu Gly 225 230 235 240 Cys Gly Ser Asp Val Cys Leu Thr Ala Thr Ala Asn Ile Gly Ser Ile                 245 250 255 Gly Leu Ser Trp Gly Ser Ser Ala Ala Leu Thr Ser Val Gln Ile Tyr             260 265 270 Arg Asp Thr Asp Ser Asn Pro Ser Gly Arg Thr Arg Ile Ala Ser Leu         275 280 285 Ser Thr Ser Ala Thr Ser Phe Thr Asp Ser Thr Thr Ala Val Gly Thr     290 295 300 Thr Tyr Tyr Tyr Trp Val Lys Tyr Gly Leu Asn Gly Ser Gln Leu Asn 305 310 315 320 Ser Asn Val Ala Ser Ala Thr Ala Leu Gln Asn Asn Thr Gly Asn Ala                 325 330 335 Ser Cys Pro Gly Glu Thr Ser Gly Glu Thr Ala Ala Thr Val Tyr Tyr             340 345 350 Val Thr Pro Asn Gly Ser Ala Ser Ala Ser Gly Asn Ser Phe Ala Ser         355 360 365 Ala Met Asp Ile Asp Thr Ala Leu Ser Ile Val Gly Ala Gly Gln Met     370 375 380 Ile Leu Met Gln Pro Gly Thr Tyr Thr Val Ala Tyr Ser Ala Gly Asn 385 390 395 400 Lys Asn Thr Lys Val Leu Ser Arg Ser Gly Ala Ala Gly Ala Pro Ile                 405 410 415 Lys Met Val Ala Ala Asn Cys Gly Arg Ala Val Phe Asp Phe Ser Phe             420 425 430 Pro Glu Arg Glu Trp Val Gln Asp Ser Tyr Gly Phe Phe Leu Thr Gly         435 440 445 Asp Tyr Trp Tyr Phe Lys Gly Ile Glu Ile Thr Arg Ala Gly Tyr Gln     450 455 460 Gly Val Tyr Val Thr Gly Ala His Asn Thr Phe Glu Asn Cys Ala Phe 465 470 475 480 Tyr Tyr Asn Arg Asn Thr Gly Leu Glu Ile Asn Lys Gly Gly Ser Tyr                 485 490 495 Thr Thr Val Ile Asn Ser Asp Ala Tyr Arg Asn Tyr Asp Pro Lys Lys             500 505 510 Asn Gly Ser Met Ala Asp Gly Phe Gly Pro Lys Gln Thr Gln Gly Pro         515 520 525 Gly Asn Lys Phe Ile Gly Cys Arg Ala Trp Glu Asn Ser Asp Asp Gly     530 535 540 Phe Asp Leu Tyr Asp Ser Pro Glu Glu Val Thr Ile Glu Asn Ser Trp 545 550 555 560 Ala Phe Arg Asn Gly Val Asp Val Trp Gly Tyr Gly Gly Phe Ala Gly                 565 570 575 Asn Gly Asn Gly Phe Lys Leu Gly Gly Asn His Val Ala Ala Asn Asn             580 585 590 Arg Ile Thr Asn Ser Val Ala Phe Gly Asn Pro Val Lys Gly Phe Asp         595 600 605 Gln Asn Asn Asn Ala Gly Gly Ile Thr Val Leu Asn Cys Thr Ala Tyr     610 615 620 Ala Asn Gly Thr Asn Tyr Gly Phe Gly Asn Asn Leu Asn Ser Gly Glu 625 630 635 640 Gln His Tyr Phe Arg Asn Asn Val Ser Val Ser Gly Ala Val Asn Ile                 645 650 655 Ser Asn Ala Asp Asn Lys Tyr Asn Ser Trp Asn Gly Gly Val Thr Ala             660 665 670 Ser Thr Ala Asp Phe Glu Asn Val Asp Leu Ser Lys Ala Thr Ala Ala         675 680 685 Arg Asn Ile Asp Gly Ser Leu Pro Asn Asn Gly Leu Phe Arg Leu Lys     690 695 700 Ser Gly Ser Asp Leu Ile Asp Ala Gly Val Glu Val Gly Leu Pro Ser 705 710 715 720 Asn Gly Ser Ala Pro Asp Met Gly Ala Phe Glu Ala Asn                 725 730 <210> 106 <211> 2202 <212> DNA <213> Microbulbifer degradans <400> 106 atgtttcgat acatccttac tgctttcgca ttggtggcag cggcttcctg cgcgcaagca 60 gctaccaatc gccctagcgg ttacaccacc atatgtaaaa ccaatcaaac ctgctctgtt 120 tcaagcccta ccaatgtggc gtttggcgca tcgggtaaat ttacctttaa agtgcttaat 180 ggctcttttg tttgtagcgt agccactttt ggctccgacc ctaacccggc taaaagtgct 240 aaagagtgct ctattccgtc ggatggctct tccagcacct ctagtacttc gagcacatcg 300 tctagttcta gtagttcatc tagcagcaca agttcaagca gcagctctag cagttcttct 360 agctcttcgt cttccagtag ctcaagttct agttcaagtg gctcttcgca agctggctgc 420 ggtagtggcg ggggagcaac ggtttgttta tcggcaaccg atacagctag tgctatcaat 480 ttaaattgga cagtgagtgg ctcgctgtcg agcgtgcagg tgtatcgcga taccgatccc 540 aacccaagtg gacgtacgcg gttaacgtcg ttaagccctt cggtaacaag ctacaccgac 600 aacaacgcac aggccggtac aacctactat tactggatta aattcggcgc aaacggcagc 660 aactataatt ccggtgctgc gtcggctgta atagccaata ccggtaacga tgatgaaggt 720 tgtggtagtg atgtgtgctt gacggcaacc gctaatattg gctctatagg cttaagctgg 780 ggctcttctg ccgctttaac cagcgtacaa atttatcgcg atacagattc aaacccaagt 840 gggcgtacac gtattgcatc gcttagcact tctgcaacca gctttaccga ttcaaccact 900 gcagtaggca caacgtatta ttactgggtt aaatacggct taaacggcag ccagctaaac 960 tctaatgttg catctgccac tgctttgcaa aataacactg gcaacgcaag ttgccccggt 1020 gaaacaagtg gcgaaactgc agcaaccgtg tattacgtaa caccaaacgg ttcggctagt 1080 gcaagcggca atagctttgc atctgcaatg gatatagaca cagcactttc aattgtaggc 1140 gcggggcaaa tgatattaat gcagcctggt acctacaccg ttgcctatag tgcgggtaat 1200 aaaaatacca aagtgctttc gcgctcgggt gccgctggtg cacctatcaa aatggtggca 1260 gccaattgcg gtcgtgcagt gtttgatttt tcgttcccag aacgtgagtg ggtgcaagat 1320 tcttacggct ttttcttaac tggcgattac tggtatttta aaggaataga aattacccgt 1380 gcaggctacc aaggtgtgta tgtaacgggt gcgcacaaca catttgaaaa ttgcgccttt 1440 tattacaacc gcaatacagg tttagaaatt aacaaaggcg ggtcttacac caccgtcatt 1500 aattcagatg cctatcgcaa ttacgatccc aagaaaaacg gcagcatggc cgatggcttt 1560 ggccctaaac aaacccaagg cccaggcaat aaatttattg gttgccgcgc gtgggaaaac 1620 tccgacgatg gatttgacct gtacgatagc ccagaagaag taactattga aaatagctgg 1680 gcatttcgca acggtgtaga tgtatggggt tacggtggtt ttgcgggtaa tggcaacggc 1740 tttaaattgg gcggtaacca cgtggctgca aacaatcgta ttaccaactc ggttgcgttc 1800 ggcaaccccg taaaaggttt tgatcaaaac aataatgccg gcggtattac agtgcttaat 1860 tgcacagcct acgccaacgg cactaactac ggctttggca acaacttaaa ctcgggtgag 1920 caacactact tccgcaataa tgtttctgta tctggcgctg tgaatattag caatgccgac 1980 aacaaataca attcgtggaa cggcggagta acagcatcca cggcagattt tgaaaacgta 2040 gatttatcca aagccaccgc tgcacgtaac atagatggca gcctgccaaa caacggccta 2100 ttccgcttaa aaagcggcag cgatttaata gacgccggtg tagaggtagg tttaccaagt 2160 aacggcagcg cgcccgatat gggagcgttc gaagctaact ag 2202 <210> 107 <211> 700 <212> PRT <213> Microbulbifer degradans <400> 107 Met Lys Asn Val Phe Asn Thr Gln Lys Thr Lys Arg His Cys Asn Tyr 1 5 10 15 Ala Tyr Asn Ala Lys Ala Ala Lys Pro Phe Ser Gln Lys Ala Leu Val             20 25 30 Gln Lys Cys Ala Ala Ala Ala Leu Ser Val Gly Leu Leu Gly Ala Val         35 40 45 Gly Asn Ala Tyr Ala Ile Ser Cys Ser Ala Thr Ala Asp Thr Trp Gly     50 55 60 Gly Gly Tyr Val Leu Asn Val Thr Val Thr Asn Asp Thr Asn Asn Ala 65 70 75 80 Ile Ser Asn Trp Ala Leu Ala Leu Asn Tyr Asp Gln Ala Ala Ala Ile                 85 90 95 Thr Asn Ser Trp Asn Ala Ser Val Ser Ala Asn Gly Asn Val Val Asn             100 105 110 Ala Thr Asn Ile Gly Trp Asn Gly Asn Leu Ala Ala Gly Gln Ser Thr         115 120 125 Ser Phe Gly Leu Gln Gly Thr Tyr Thr Gly Asn Phe Ser Leu Pro Val     130 135 140 Cys Val Gly Gln Gly Gln Ser Ser Ser Ser Ser Ser Thr Ser Ser 145 150 155 160 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser                 165 170 175 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser             180 185 190 Ser Ser Ser Ser Thr Ser Ser Thr Gly Gly Ser Ser Glu Leu Thr Ile         195 200 205 Gln Glu Asp Asn Ser Gly Phe Cys Gly Val Asp Gly Ser Ile Asp Ser     210 215 220 Asn Asn Ser Gly Phe Thr Gly Ser Gly Phe Ala Asn Thr Asp Asn Ala 225 230 235 240 Thr Gly Lys Ser Val Asp Trp Ser Val Ser Val Pro Tyr Ser Gly Asn                 245 250 255 Tyr Leu Leu Glu Trp Arg Tyr Ala Asn Gly Ser Gly Asn Asn Arg Ala             260 265 270 Gly Ala Ile Glu Val Asn Gly Asn Ala Arg Gly Asn Gln Ser Phe Pro         275 280 285 Thr Thr Gly Ala Trp Thr Ser Trp Thr Thr Ala Ser Ala Asn Val Ser     290 295 300 Leu Asp Ala Gly Thr Asn Leu Ile Ser Leu Val Ala Ser Thr Gly Glu 305 310 315 320 Gly Leu Gly Asn Ile Asp Ser Leu Thr Val Ile Gly Asn Asp Ile Gln                 325 330 335 Thr Gly Ala Cys Asp Ser Thr Gly Ser Ser Ser Ser Ser Ser Ser Ser             340 345 350 Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser         355 360 365 Ser Ser Ser Ser Gly Ala Pro Met Leu Pro Gln Ala Gly Asn Pro Ile     370 375 380 Asn Gly Lys Phe Gly Lys Tyr Lys Ser Trp Gln Lys Gly Ser Leu Ser 385 390 395 400 Ala Asp Lys Gln Phe Ala Asp Ile Leu Leu Ser His Gln Tyr Thr Asn                 405 410 415 Gly Gly Phe Pro Lys Asn Gln Ala Tyr Asp Ser Met Gly Ser Gly Gly             420 425 430 Asn Ser Ala Gly Thr Ile Asp Asn Asp Ala Thr Thr Thr Glu Leu Leu         435 440 445 Phe Leu Ala Asp Val Tyr Gln Arg Thr Gly Glu Thr Lys Tyr Arg Asp     450 455 460 Gly Ala Arg Lys Ala Leu Asp Phe Leu Leu Asp Met Gln Tyr Ser Ser 465 470 475 480 Gly Gly Trp Pro Gln Tyr Tyr Pro Val Arg Ser Gly Tyr Tyr Glu His                 485 490 495 Val Thr Phe Asn Asp Asp Ala Met Ala Arg Val Leu Ile Val Leu Asp             500 505 510 Lys Ala Lys Gln Gly Val Ala Pro Leu Asn Gly Asp Leu Leu Thr Ser         515 520 525 Asn Gln Arg Ala Arg Leu Ser Ser Ala Val Asn Lys Gly Val Asp Tyr     530 535 540 Ile Leu Lys Ser Gln Trp Arg Gln Asn Gly Thr Leu Thr Val Trp Cys 545 550 555 560 Ala Gln His Gly Lys Asp Asp Tyr Leu Pro Lys Lys Ala Arg Ala Tyr                 565 570 575 Glu Leu Glu Ser Leu Ser Gly Ser Glu Ser Val Leu Val Val Ala Phe             580 585 590 Leu Met Ser Gln Pro Gln Thr Pro Glu Ile Lys Thr Ala Val Lys Ala         595 600 605 Ala Ile Asn Trp Phe Arg Ser Pro Asn Thr Tyr Leu Ala Gly Tyr Thr     610 615 620 Tyr Asp Ser Ser Arg Lys Gly Asp Gly Asn Ser Pro Ile Val Ala Lys 625 630 635 640 Ser Gly Ser Lys Met Trp Tyr Arg Phe Tyr Asp Leu Asn Thr Asn Arg                 645 650 655 Gly Phe Phe Ser Asp Arg Asp Ser Arg Lys Val Tyr Asp Ile Leu Asp             660 665 670 Ile Ser Thr Glu Arg Lys Asp Gly Tyr Arg Trp Gly Gly Asp Tyr Gly         675 680 685 Ser Gly Ile Ile Ser Tyr Ala Glu Ser Val Gly Tyr     690 695 700 <210> 108 <211> 72 <212> PRT <213> Microbulbifer degradans <400> 108 Met Val Asn Ser Leu Leu Pro Pro Val Glu Leu Val Asp Glu Asp Asp 1 5 10 15 Glu Leu Glu Asp Glu Glu Leu Leu Glu Leu Leu Glu Glu Leu Leu Glu             20 25 30 Glu Leu Glu Asp Glu Leu Leu Asp Glu Leu Glu Glu Leu Asp Glu Leu         35 40 45 Leu Leu Val Leu Leu Leu Glu Glu Glu Leu Trp Pro Trp Pro Thr Gln     50 55 60 Thr Gly Lys Leu Lys Leu Pro Val 65 70 <210> 109 <211> 2103 <212> DNA <213> Microbulbifer degradans <400> 109 atgaaaaacg tatttaatac acaaaaaacc aagcggcatt gcaactatgc ctataacgcg 60 aaagctgcca aacccttcag ccaaaaagca ctggtacaaa agtgtgcggc tgcggcattg 120 tctgttggct tactgggggc cgtgggtaat gcgtatgcaa tatcctgttc ggcaactgct 180 gatacctggg gtggtggcta tgtgctaaat gtgaccgtta ctaacgacac aaataatgca 240 attagcaatt gggcgctagc tttaaattac gatcaagctg cagccataac taattcgtgg 300 aacgcgagtg tgtctgcaaa tggcaatgtg gttaatgcta ccaacattgg ttggaatggc 360 aatttagcgg ctggccaaag tacaagtttt ggtttgcaag gcacctatac cggcaacttt 420 agtttacctg tctgtgttgg ccagggccaa agctcttcct ctagtagcag tactagtagt 480 agttcgtcca gctcttcgag ttcatccagc agttcatctt ccagctcttc aagtagttct 540 tcaagcagct ctagtagttc ttcatcttct agctcatcgt cttcgtccac cagttcaact 600 ggtggaagta gtgagttaac cattcaagaa gataatagcg gcttttgcgg ggttgatggt 660 tcaatcgatt ccaataattc aggctttacc ggaagcggct tcgccaatac cgataacgcg 720 acaggcaaaa gcgtagattg gagtgtgagc gttccttata gtggcaacta tttgcttgaa 780 tggcgttacg caaatggttc cggtaataac cgtgctggcg cgattgaagt aaatggtaac 840 gctcgtggta atcaaagctt tcctactaca ggggcctgga ctagctggac aacggcaagt 900 gctaacgtaa gtttggatgc aggtacaaac ttaattagct tggttgcatc tacgggcgaa 960 ggcttaggga atattgattc acttactgtc attggtaacg atattcagac cggcgcttgt 1020 gattctacag ggtctagctc ttccagtagc tctagttcct ccagtacttc tagctcaagt 1080 tccagctcca gttcaagtac cagtagttct agcagcggtg cgcccatgtt acctcaagct 1140 gggaacccca ttaatggcaa gtttggcaaa tataaatctt ggcaaaaagg aagcttgtct 1200 gccgataagc aatttgcaga catccttcta tcgcaccaat ataccaatgg cggatttccc 1260 aaaaaccaag cctacgacag tatgggtagc ggtggtaaca gtgcgggcac aatcgacaac 1320 gatgccacaa caacagagtt gttattctta gctgatgtgt accagcgtac tggtgaaacc 1380 aaataccgag acggtgcgcg taaagcgtta gatttccttt tggatatgca gtattcatcg 1440 ggcggctggc cgcaatacta ccctgtgcgc agtggctact acgagcatgt aacatttaac 1500 gacgatgcaa tggcgcgagt gctaattgtt ttagataaag cgaaacaagg tgtggcgccg 1560 ctaaatggcg atctattaac atctaaccag cgtgcgcgtt taagcagtgc ggttaataag 1620 ggcgtggatt acattcttaa atcgcagtgg cgtcaaaacg gaaccttaac tgtttggtgt 1680 gcgcaacatg gtaaagatga ttatctacct aaaaaggcgc gtgcttacga gctggaatcg 1740 ttgagtggta gcgaatcggt attggtagtt gcattcctta tgtctcaacc tcaaacccct 1800 gaaattaaaa cggcggttaa ggctgctatc aattggttta gaagccccaa tacttactta 1860 gctggttaca cttacgattc atctagaaaa ggcgacggca acagccccat cgtcgcgaaa 1920 agcggtagta aaatgtggta tcgcttctac gacctaaata ctaaccgtgg cttcttcagt 1980 gatagagata gcagaaaagt ttacgatatt ttagatattt ctacagagcg taaagatggc 2040 tatcgttggg gcggtgatta tggctccggc atcattagtt acgcggaaag cgttggttac 2100 taa 2103 <210> 110 <211> 96 <212> PRT <213> Microbulbifer degradans <400> 110 Met Ser Val Pro Ala Leu Pro Pro Glu Glu Leu Leu Glu Leu Glu Asp 1 5 10 15 Glu Leu Leu Glu Leu Glu Asp Glu Leu Leu Glu Leu Asp Glu Leu Leu             20 25 30 Glu Leu Asp Glu Leu Leu Glu Leu Val Glu Leu Leu Glu Leu Glu Glu         35 40 45 Leu Val Glu Ala Phe Ile Ser Leu Ala Gly Pro Glu Leu Pro Pro Pro     50 55 60 Gln Ala Leu Asn Ser Val Ala Ala Ser Ala Ser Val Ile Arg Arg Arg 65 70 75 80 Lys Phe Ile Ile Val Ala Leu Tyr Val Phe Ile Leu Arg Ala His Lys                 85 90 95 <210> 111 <211> 574 <212> PRT <213> Microbulbifer degradans <400> 111 Met Asn Phe Leu Arg Leu Ile Thr Leu Ala Leu Ala Ala Thr Leu Leu 1 5 10 15 Ser Ala Cys Gly Gly Gly Ser Ser Gly Pro Ala Lys Glu Ile Asn Ala             20 25 30 Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser         35 40 45 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser     50 55 60 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly Gly Ser Ala Gly 65 70 75 80 Thr Leu Thr Ile Gln Glu Ser Glu Lys Gly Phe Cys Thr Val Asn Gly                 85 90 95 Glu Ile Val Asn Asn His Glu Gly Tyr Ser Gly Thr Gly Phe Val Asp             100 105 110 Thr Ala Asn Ala Glu Gly Ala Ser Ile Thr Trp Lys Val Asp Val Asp         115 120 125 Gly Gly Asn Tyr Asp Val Ser Val Arg Phe Ala Asn Gly Ser Thr Ala     130 135 140 Arg Gly Ala Thr Leu Ser Ser Asn Glu Ile Asn Thr Thr Tyr Gly Phe 145 150 155 160 Ala Thr Thr Gly Asp Trp Ala Thr Trp Ala Asp Glu Thr His Thr Val                 165 170 175 Ser Leu Ala Ala Gly Glu Asn Thr Ile Gln Leu Ser Ala Leu Thr Ala             180 185 190 Gly Gly Leu Pro Asn Val Asp Ala Ile Thr Ile Ala Gly Ala Gly Val         195 200 205 Leu Ala Ala Asp Cys Ala Thr Glu His Thr Gly Pro Met Leu Ser Gln     210 215 220 Thr Gly Asn Pro Ile Tyr Thr Glu Leu Asn Asn Tyr Lys Ser Trp Leu 225 230 235 240 Thr Gly Ser Gly Thr Thr Ala Ala Lys Leu Ala Ala Asp Lys Thr Ile                 245 250 255 Ala Asp Asn Met Ile Thr Trp Gln Met Pro His Gly Gly Phe Tyr Lys             260 265 270 Tyr Gly Val Ser Lys Tyr Ser Ser Ala Trp Asn Gly Ser Asp Ala Arg         275 280 285 Ser Gly Trp Thr Gly Ala Asn Gly Val Glu Leu Gly Thr Ile Asp Asn     290 295 300 Asp Ala Thr Val Ser Glu Leu Leu Phe Leu Ala Asp Val Tyr Lys Arg 305 310 315 320 Ser Gly Glu Thr Lys Tyr Arg Asp Ala Ala Arg Ser Ala Leu Glu Phe                 325 330 335 Leu Leu Thr Met Gln Tyr Ser Thr Gly Grp Trp Pro Gln Val Tyr Pro             340 345 350 Ala Arg Thr Gly Thr Ser Tyr Ser Asn His Val Thr Phe Asn Asp Asn         355 360 365 Ala Met Ala Arg Val Leu Ile Leu Leu Asp Lys Ala Ala Arg Leu Glu     370 375 380 Ala Pro Leu Asp Gly Asp Ile Phe Thr Thr Asp Gln His Thr Arg Ile 385 390 395 400 Thr Thr Ala Ile Asn Gly Gly Ile Asp Phe Ile Leu Asn Ala Gln Ile                 405 410 415 Val Gln Gly Asp Val Lys Thr Val Trp Cys Ala Gln His Asp Pro Tyr             420 425 430 Thr Tyr Glu Ala Lys Ala Ala Arg Ser Tyr Glu Leu Ala Ser Lys Ser         435 440 445 Gly Lys Glu Ser Val Leu Val Val Ala Phe Leu Met Thr Arg Pro Gln     450 455 460 Ser Glu Ala Ile Glu Asn Ala Val Lys Ala Ala Leu Ala Trp Tyr Arg 465 470 475 480 Asn Pro Asn Val Gln Val Ala Asn Thr Glu Tyr Val Lys Arg Thr Asn                 485 490 495 Asn Asp Asp Asn Tyr Asn Pro Ile Gln Thr Lys Ala Gly Ser Thr Met             500 505 510 Trp Tyr Arg Phe Tyr Asp Leu Asp Gln Asp Val Gly Phe Phe Ser Gly         515 520 525 Arg Ser Ala Ser Asp Asn Pro Ala Gly Asn Gly Lys Gln Tyr Asp Ile     530 535 540 Met Leu Ile Glu Pro Glu Arg Arg Tyr Gly Tyr Glu Trp Gly Gly Asn 545 550 555 560 Tyr Gly Lys Lys Ile Ile Asp Tyr Ala Asn Ser Val Gly Tyr                 565 570 <210> 112 <211> 1725 <212> DNA <213> Microbulbifer degradans <400> 112 atgaattttc tacgccttat tacactcgca ctcgccgcaa cactattaag tgcctgcggt 60 ggaggtagtt ctggcccagc caaagagata aacgcttcca ccagttcctc tagctctagc 120 agctccacga gttctagcag ctcatccagc tctagtagtt cgtctagctc aagcagctcg 180 tcttccagtt ccagcagctc atcctctagc tctagcagct cttctggtgg tagcgccggc 240 acactcacta ttcaggaaag cgaaaaaggc ttttgcactg ttaacggtga gattgtgaat 300 aatcacgagg ggtatagcgg tacaggtttt gtagacaccg ccaatgccga aggcgccagc 360 attacttgga aggtagatgt cgatggcggc aattatgatg taagtgtgcg attcgcaaac 420 ggttccactg cacgcggcgc aacgcttagc agtaacgaaa taaataccac ctatggtttt 480 gctaccactg gcgactgggc cacatgggca gacgaaaccc acaccgtttc gttagccgca 540 ggcgaaaaca ccatccagct aagtgcactt accgcgggtg gcttacccaa tgtagacgct 600 attactattg caggtgcagg tgtactcgca gcagactgcg ccacagagca tactgggcca 660 atgctttcgc aaacaggcaa ccctatttat accgagttaa ataattacaa gtcctggcta 720 acgggtagcg gcacaacagc agccaaatta gccgcagata aaacaattgc cgacaatatg 780 attacctggc aaatgcctca cggtggtttt tataaatacg gcgtatctaa atacagctcg 840 gcttggaacg gtagcgatgc ccgctctggc tggactgggg ccaacggcgt tgagcttggc 900 acaattgata atgatgcaac cgttagcgaa ttattatttt tagcggacgt atataaacgc 960 agcggtgaaa ctaaatatag agatgccgca agaagcgcgt tggaattttt acttaccatg 1020 caatattcca ctggcggttg gccacaggtt taccctgcgc gcactggcac cagttactcc 1080 aatcacgtta cgtttaacga taacgccatg gctcgtgtac ttattttatt ggataaagcc 1140 gcgcgattag aagcaccact cgatggcgac atttttacca cagaccagca cacgcgtatt 1200 actaccgcaa taaatggcgg catcgatttt attttgaatg cgcaaatagt acagggcgac 1260 gtgaaaaccg tttggtgtgc gcaacacgac ccttatacct acgaggcaaa agcagctcgc 1320 tcttatgagt tggcctctaa aagcggtaaa gaatctgtat tggttgtagc gtttttaatg 1380 acacgcccgc aaagcgaagc catagaaaat gccgtgaaag cagcccttgc ttggtaccgc 1440 aacccaaatg ttcaagtcgc caacaccgag tatgtaaaac gcacaaataa cgatgacaac 1500 tacaacccga tacaaacgaa agcaggtagc actatgtggt accgctttta cgatttagac 1560 caagacgttg gattctttag cggccgctct gcaagtgaca acccagcagg taacggtaag 1620 caatacgaca ttatgcttat tgaacccgag cgcaggtatg gctatgaatg gggtggcaat 1680 tacggcaaaa aaataatcga ttacgctaat tcggtagggt attaa 1725 <210> 113 <211> 463 <212> PRT <213> Microbulbifer degradans <400> 113 Met Tyr Lys Ile Ser Arg Arg Thr Thr Leu Lys Gly Leu Gly Leu Thr 1 5 10 15 Cys Leu Ala Gly Cys Thr Thr Ser Leu Pro Thr Leu Glu Gln Asp Pro             20 25 30 Trp Ala Phe Ala Gln Asn Ile Ala Asp Asn Thr Thr Ile Pro Thr Phe         35 40 45 Pro Asn Lys Glu Phe Asn Leu Leu Glu Phe Gly Gly Lys Glu Gly Ser     50 55 60 Asp Asn Thr Leu Ala Phe Lys Lys Ala Ile Ala Ala Cys Ser Lys Ala 65 70 75 80 Gly Gly Gly Lys Val Val Val Pro Ala Gly Arg Phe Glu Thr Gly Ala                 85 90 95 Ile His Leu Glu Ser Asn Val Asn Leu His Ile Ser Glu Gly Ala Thr             100 105 110 Ile Ala Phe Phe Thr Asp Pro Lys Tyr Tyr Leu Pro Ala Val Phe Thr         115 120 125 Arg Trp Glu Gly Met Glu Cys Met Gly Tyr Ser Pro Leu Ile Tyr Ala     130 135 140 Tyr Gly Lys Thr Asn Ile Ala Ile Thr Gly Lys Gly Thr Leu Asp Gly 145 150 155 160 Gln Ala Asp Pro Thr His Trp Trp Ala Trp Lys Gly Asn Lys Glu Trp                 165 170 175 Gly Val Glu Gly Tyr Pro Ser Gln Lys Glu Ser Arg Asn Gln Leu Phe             180 185 190 Ala Gln Ala Glu Ala Gly Asp Pro Val Arg Glu Arg Val Tyr Ala Asp         195 200 205 Gly His Tyr Leu Arg Pro Ser Phe Val Gln Pro Tyr Lys Cys Glu Asn     210 215 220 Val Leu Ile Glu Asp Ile Thr Ile Ile Asn Ala Pro Phe Trp Leu Leu 225 230 235 240 His Pro Thr Leu Ser Gln Asn Val Thr Val Arg Gly Val His Leu Glu                 245 250 255 Ser Leu Gly Pro Asn Ser Asp Gly Cys Asp Pro Glu Ser Cys Lys Asn             260 265 270 Val Val Ile Glu Asn Cys Phe Phe Asn Thr Gly Asp Asp Cys Ile Ala         275 280 285 Ile Lys Ser Gly Arg Asn Asn Asp Gly Arg Arg Leu Ala Thr Pro Thr     290 295 300 Glu Asn Val Ile Ile Arg Asn Cys Lys Met Glu Ala Gly His Gly Gly 305 310 315 320 Val Val Ile Gly Ser Glu Ile Ser Gly Gly Val Arg Asn Val Phe Ala                 325 330 335 Glu Asn Asn Val Met Ser Ser Pro Asp Leu Glu Lys Gly Ile Arg Ile             340 345 350 Lys Thr Asn Ser Val Arg Gly Gly Leu Leu Glu Asn Ile Tyr Val Arg         355 360 365 Asn Cys Thr Ile Gly Glu Val Gln Gln Ala Ile Val Ile Asn Phe Gln     370 375 380 Tyr Glu Glu Gly Asp Ala Gly Lys Phe Asp Pro Thr Val Arg Asn Val 385 390 395 400 Glu Ile Arg Asn Leu Val Cys Gln His Ala Leu Gln Val Phe Asn Ile                 405 410 415 Arg Gly Phe Glu Arg Ala Pro Ile Gln Asn Phe Arg Ile Ile Asp Ser             420 425 430 Thr Phe Val Arg Gly Asp Asn Pro Gly Val Ile Glu His Thr Thr Gly         435 440 445 Leu Val Ile Asp Asn Val Gln Val Asn Gly Lys Ala Phe Asn Ile     450 455 460 <210> 114 <211> 1392 <212> DNA <213> Microbulbifer degradans <400> 114 atgtataaaa tttcacgccg cacaacactc aaaggcttag gcctaacttg cctagccggc 60 tgcaccacca gcctacccac actagagcaa gacccatggg cttttgcaca aaacatagcg 120 gacaacacca ccatccccac attcccaaac aaagaattta atttactcga attcggcggc 180 aaagaaggga gcgacaacac cctcgccttc aaaaaagcga ttgcagcatg cagcaaagca 240 ggtggcggca aggtggtagt acccgcagga cgatttgaga caggcgccat ccacttagag 300 tcgaacgtta accttcatat tagcgaaggc gctaccatcg ccttttttac cgaccccaaa 360 tattacctgc ctgcggtttt cactcgctgg gaaggcatgg agtgcatggg ctactcaccc 420 cttatatacg cctacggcaa aaccaacata gccattaccg gtaaaggcac cctcgacggt 480 caagccgacc caacgcactg gtgggcatgg aaaggcaaca aagaatgggg cgtagagggc 540 tacccaagcc aaaaggaaag ccgcaaccaa ctatttgccc aagcagaagc tggcgacccc 600 gttagagagc gcgtgtatgc agacggccac tacctgcgcc cctcgtttgt gcaaccctac 660 aagtgcgaaa acgtgctgat agaagacata actattatca acgctccctt ctggttgcta 720 caccccaccc tttcacaaaa cgtcactgta cgcggtgttc acctagaaag cctaggcccc 780 aactcggatg gctgcgatcc tgaaagctgt aagaatgtag ttatcgaaaa ctgctttttt 840 aataccggtg acgactgtat cgctattaaa tctggccgca acaacgatgg ccgcaggctt 900 gccacaccta ccgagaacgt gattattcgc aactgtaaaa tggaagcggg tcacggtggc 960 gtagttatag gctcagaaat ttctggcggc gtgcgcaatg tgtttgccga aaataacgta 1020 atgagcagcc ccgatttaga gaaaggcatt cgcattaaaa ccaactctgt gcgcggcgga 1080 ctgctagaga acatctatgt gcgcaactgc accataggcg aagtacaaca agccattgtt 1140 attaacttcc aatacgaaga aggcgatgcg ggtaaatttg accccaccgt gcgcaatgta 1200 gaaatacgca atttggtctg ccagcacgcc ttacaagtgt ttaacatccg cggttttgag 1260 cgcgccccca ttcaaaactt taggataatc gacagcacct ttgtgcgtgg tgacaaccca 1320 ggcgtaattg aacataccac agggttagtt atcgacaacg tccaagtcaa cggcaaagcg 1380 tttaacatct ag 1392 <210> 115 <211> 1084 <212> PRT <213> Microbulbifer degradans <400> 115 Met Leu Asp Met Thr Lys Arg Thr Leu Ser Ala Leu Leu Ala Leu Cys   1 5 10 15 Ala Thr Leu Thr Ala Cys Gly Gly Gly Asp Ile Thr Ser Gly Gly Asp              20 25 30 Ala Ile Pro Ala Val Asn Gln Pro Ala Pro Val Gln Glu Pro Glu Pro          35 40 45 Glu Pro Glu Pro Gln Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro      50 55 60 Glu Pro Glu Gly Ala Trp Thr Cys Pro Glu Thr Gly Phe Tyr Phe Cys  65 70 75 80 Asp Asp Phe Glu Asp Gly Thr Phe Asp Asp Lys Trp Asp Asp Leu Ile                  85 90 95 Ala Thr Tyr Asp Leu Pro Ser Pro Gly Val Phe Asp Ile Leu Asp Glu             100 105 110 Ala Ser Gly Lys Ser Leu Arg Phe Thr Ala Gly Thr Arg Gly Gly Asp         115 120 125 Leu Ala Asp Gly Glu Leu Ile Val Val Lys Asp Thr Ala Phe Glu Asn     130 135 140 Val Thr Asn Ala Asp Tyr Ser Leu Glu Tyr Arg Ile Arg Pro Arg Asn 145 150 155 160 Asn Gly Asn Thr Gly Asn Lys Tyr Leu His Ala Met Ser Arg Tyr Glu                 165 170 175 Gly Pro Lys Glu Tyr Tyr Phe Gly Gly Leu Ser Met Gln Gly Ser Thr             180 185 190 Ala Ser Thr Gln Val Glu Ala Gly Phe Val Leu Pro Glu Asn Thr Thr         195 200 205 Ser Ile Ser Asn Arg Leu Val Gln Ala Lys Tyr Pro Leu Glu Leu Gly     210 215 220 Thr Thr Gly Met Ser Asp Gly Tyr Trp Tyr Glu Val Arg Phe Asp Met 225 230 235 240 Ile Gly Asn Thr Gly Thr Ile Tyr Leu Asp Gly Glu Pro Gln Gly Ser                 245 250 255 Phe Thr Asp Ala Asp Gly Leu Tyr Pro Leu Thr Gly Lys Ile Gly Phe             260 265 270 Met Thr Tyr Asn Arg Ser Phe Glu Ile Asp Trp Val Arg Val Gly Asp         275 280 285 Pro Ala Ile Lys Pro Val Gln Leu Ser Leu Asp Tyr Ala Ser Pro Leu     290 295 300 Trp Glu Ala Ala Ala Asp Gln Asp Pro Leu Asn Val Thr Val Thr Ala 305 310 315 320 Ile Gln Ser Asp Gly Val Thr Ala Asp Thr Phe Thr Ala Val Ser Ser                 325 330 335 Asp Thr Asn Val Val Thr Thr Ser Ile Ala Asn Asn Val Val Thr Ile             340 345 350 Thr Pro Val Ala Gln Gly Ser Ala Thr Val Thr Phe Thr Ala Gly Ser         355 360 365 Asp Ala Asn Arg Val Lys Thr Ile Asp Val Glu Ile Ala Arg Ala Phe     370 375 380 Val Met Ser Thr Thr Asp Tyr Gly Asp Ile Ala Ser Lys Val Thr Pro 385 390 395 400 Thr Val Gly Met Thr Asp Ala Asn Pro Asp Ala His Leu Ser Ile Thr                 405 410 415 Phe Asp Ser Ala Pro Thr Leu Ser Gly Val Gly Ser Ile Arg Ile Tyr             420 425 430 Asn Ala Ala Asp Asp Ser Glu Val Asp Val Ile Arg Leu Thr Asp Glu         435 440 445 Ser Asp Ala Leu Gly Tyr Ala Gly Gln Ala Asn Lys Arg Glu Leu Asn     450 455 460 Thr Thr Pro Val Tyr Leu Asp Gly Asn Thr Leu His Val Ser Pro His 465 470 475 480 Ser Asn Ala Leu Ala Tyr Gly Gln Asp Tyr Tyr Val Ala Ile Gly Asp                 485 490 495 Asn Val Leu Thr Gly Ala Thr Leu Asn Thr Ile Ala Phe Asp Gly Leu             500 505 510 Gly Lys Asn Ala Gly Trp Thr Phe Ser Thr Lys Ala Ser Ala Pro Thr         515 520 525 Gly Asn Thr Val Thr Val Asp Asp Asp Ala Ser Ala Asp Phe Ser Thr     530 535 540 Val Gln Gly Ala Leu Asn Tyr Ala Met Ala Asn Thr Thr Asp Asp Ser 545 550 555 560 Ile Thr Ile Asn Ile Ala Asn Gly Asn Tyr Tyr Glu Pro Leu Tyr Leu                 565 570 575 Ala Glu Arg Asn Asn Val Thr Leu Lys Gly Glu Ser Arg Asp Gly Val             580 585 590 Val Ile His Tyr Asn Asn His Glu Ala Met Asn Gly Gly Ser Thr Gly         595 600 605 Arg Ala Asn Phe Tyr Val Ala Asn Ser Asp Met Leu Thr Leu Glu Thr     610 615 620 Leu Thr Leu Lys Asn Gly His Gln Arg Thr Gly Gly Gly Asp Gln Ala 625 630 635 640 Glu Thr Ile Tyr Phe Asn Ser Ser Ser Asn Thr Asp Arg Leu Ile Ala                 645 650 655 Lys Gly Ala Ala Phe Ile Ser Glu Gln Asp Thr Leu Leu Leu Lys Gly             660 665 670 Tyr Asn Trp Phe Tyr Asn Ser Leu Val Val Gly Asn Val Asp Phe Ile         675 680 685 Trp Gly Tyr Ser Ala Val Thr Leu Phe Glu Glu Thr Glu Ile Arg Ser     690 695 700 Ile Ala Asp Ser Lys Pro Gly Ala Gly Asp Ser Gly Gly Tyr Ile Leu 705 710 715 720 Gln Ala Arg Thr Pro Leu Glu Thr Asp Leu Gly Phe Val Phe Leu Asn                 725 730 735 Ser Glu Leu Thr Lys Ala Thr Gly Val Asn Gly Asn Glu Ile Gly Asp             740 745 750 Gly Lys Thr Tyr Leu Ala Arg Ser Gly Gly Ser Thr Gly Tyr Phe Asp         755 760 765 Asn Ile Ser Phe Ile Asn Thr Lys Met Gly Ser His Ile Ala Asp Ile     770 775 780 Gly Phe Ala Tyr Ala Asp Ile Asn Gly Gln Pro Ala Pro Asn Pro Ala 785 790 795 800 Val Ala Thr Ala Asp Ala Gly Trp Arg Glu Phe Gly Ser Met Asp Ser                 805 810 815 Ala Gly Thr Ala Leu Asp Val Ser Ala Arg Cys Gly Asp Ser Gly Ser             820 825 830 Cys Ile Gln Leu Thr Gln Ala Gln Val Asp Ala Gln Tyr Cys Asn Arg         835 840 845 Ala Gln Ile Phe Ala Ser Trp Asn Asp Trp Thr Gly Trp Asp Pro Leu     850 855 860 Pro Glu Asp Thr Ser Asp Asp Ala Cys Ala Asp Pro Val Ile Pro Gly 865 870 875 880 Ala Val Thr Trp Thr Gly Ile Ala Met Ser Leu Gly Gly Ser Thr Thr                 885 890 895 Ser Val Ser Gly Asn Ile Thr Glu Gln Thr Asp Ser Asn Ile Thr Phe             900 905 910 Thr Ala Asp Gly Gly Lys Phe Glu Ser Ser Lys Leu Ser Thr Tyr Phe         915 920 925 Ala Tyr Gln Glu Leu Thr Gly Asp Phe Val Ile Ser Ala Lys Ala Lys     930 935 940 Thr Ile Gly Leu Leu Arg Glu Asn Gly Ser Tyr Gln Phe Pro Thr Gly 945 950 955 960 Ile Leu Met Cys Val Cys Asp Ala Ala Ala Ala Thr Thr Gly Leu Met                 965 970 975 Gly His Ala Ser Leu Asn Asp Ile Thr Val Asp Thr Thr Val Asn Leu             980 985 990 Val Ala Thr Tyr Gly His Ile Gln Thr Thr Ala Gly Ser Trp Asn Lys         995 1000 1005 Thr Gly Thr Thr Asp Val Thr Ala Gly Asp Asn Leu Tyr Ile Gln Leu    1010 1015 1020 Glu Arg Ala Gly Asn Ser Tyr Thr Ala Arg Tyr Ser Thr Asp Gly Gly 1025 1030 1035 1040 Ala Thr Tyr Ser Asn Ile Gly Gly Ser Ser Phe Thr Asp Thr Leu Pro                1045 1050 1055 Asp Thr Leu Lys Val Gly Phe Phe Ala Thr Pro Asn Asn Thr Gly Glu            1060 1065 1070 Gln Thr Phe Val Tyr Glu Asp Ile Gln Ile Thr Gln        1075 1080 <210> 116 <211> 3255 <212> DNA <213> Microbulbifer degradans <400> 116 atgctcgata tgacaaaacg aactctatct gcgttgttag ccctgtgcgc aacattaacg 60 gcttgcggtg gtggcgatat aaccagcggc ggcgatgcta taccggcagt aaaccaaccc 120 gccccagtac aagagcctga acctgaacct gaaccacaac cggaacccga acccgaaccc 180 gagcccgagc cagaaccaga gggcgcgtgg acctgcccag aaacaggctt ctacttctgt 240 gacgactttg aagacggcac gtttgatgac aagtgggacg atctcattgc cacatacgac 300 ctaccaagcc ctggtgtatt cgacatatta gacgaagcaa gcggcaaatc tttgcgcttt 360 acagcaggca cccgtggcgg tgacttagca gatggcgaac ttattgttgt aaaagataca 420 gcattcgaaa atgtaaccaa cgcagattac tccttagagt accgtattcg cccgcgcaac 480 aacggcaaca caggcaacaa gtacctgcac gctatgtcgc gctacgaagg ccctaaagaa 540 tattactttg gcggtttaag catgcaaggc tctactgcaa gtacgcaagt agaagcaggt 600 ttcgtattgc cagaaaacac cactagcatt agcaaccgct tggtgcaggc caagtacccg 660 ttagagctag gtacaacagg catgagcgac ggctactggt acgaagtacg cttcgatatg 720 ataggcaata caggcaccat ttacctagat ggcgaaccac aaggcagctt taccgatgcc 780 gatggccttt acccattaac aggtaaaatt ggctttatga cttacaaccg ctcattcgaa 840 attgattggg tgcgagtagg cgacccagct attaagcctg tacaactttc actggattac 900 gccagcccgc tatgggaagc agcggcagac caagacccgc taaacgtaac agttactgcc 960 atacaaagcg atggcgtaac agcagatacc tttaccgcag ttagcagcga taccaatgta 1020 gtaaccacaa gtattgcaaa taacgtagta accattaccc ctgtagctca aggtagtgcc 1080 accgtgacct ttaccgctgg ttcagatgct aatcgcgtta aaacaattga tgtagaaatt 1140 gcacgcgcgt ttgttatgtc tactaccgac tacggcgata tagcttctaa ggtaacacca 1200 actgttggta tgactgacgc caacccagac gcacatttaa gcattacatt cgatagcgca 1260 cctaccctaa gcggtgttgg ctctatacgt atatacaatg cagcagacga tagcgaagta 1320 gatgttattc gccttaccga cgaaagtgat gcattgggtt acgccggcca agccaacaag 1380 cgtgaattaa ataccacacc ggtttacttg gatggcaaca ccctacacgt tagcccacac 1440 agtaacgcac ttgcctacgg ccaagactac tacgttgcca ttggcgataa cgtacttacc 1500 ggcgcaacac taaacaccat tgcgtttgat ggtttaggta aaaacgcggg ttggactttc 1560 tctaccaaag cctctgcccc taccggcaac accgtaactg tagacgacga tgcaagtgca 1620 gatttcagca cagtacaagg tgcgttgaac tatgctatgg caaataccac ggacgattca 1680 atcaccatta acattgctaa cggcaactac tacgagccgc tatatctagc agagcgcaac 1740 aacgtaacgc taaaaggtga aagccgcgac ggcgttgtta ttcattacaa caaccacgaa 1800 gccatgaacg gtggcagcac tggccgcgca aacttctatg ttgccaactc agacatgcta 1860 accctagaaa cgctaaccct taaaaacggt catcagcgca ctggtggtgg cgaccaagca 1920 gaaactatct acttcaatag cagcagcaat accgatcgct taattgccaa aggcgctgct 1980 tttattagtg aacaagatac gctgttactt aaaggctaca actggttcta caactcgctt 2040 gtggtaggta acgtagactt tatttggggc tacagcgcag taaccttgtt tgaagaaaca 2100 gaaattcgat ctattgccga ctctaaacca ggtgcgggcg actcgggtgg ctatattctg 2160 caagcgcgta cgccactaga aacagacctt ggctttgttt tcttaaatag cgaattaaca 2220 aaagctaccg gcgtaaacgg taacgaaatt ggcgatggca aaacctacct tgcgcgcagc 2280 ggcggcagca cgggttactt cgataatatt tcgtttatta acaccaaaat gggtagccat 2340 attgccgaca taggcttcgc ctacgccgac attaacggtc aacctgcccc taacccagcg 2400 gtagctactg ctgacgcagg ctggcgtgaa tttggcagca tggattctgc aggcacggct 2460 ctagatgtat ctgcacgctg cggtgatagc ggcagctgta tccaacttac gcaagcacaa 2520 gtagatgcgc agtactgtaa ccgcgcgcaa atttttgcta gctggaacga ttggacaggc 2580 tgggacccgt tgccagaaga tacctctgac gatgcctgtg ctgaccccgt tatacctggt 2640 gcagtaacgt ggactggcat tgcaatgagc cttgggggtt ctacaacatc tgtttccggc 2700 aacattaccg agcaaacaga cagcaatatt acattcactg cagacggcgg taagtttgaa 2760 tcgagcaaac tttcaactta cttcgcttat caagaattga ctggcgactt tgtaattagc 2820 gccaaagcta aaaccattgg cttactgcgc gaaaacggca gctaccagtt ccctacaggc 2880 atattgatgt gtgtttgcga tgcggcagcg gcaacaactg gcttaatggg ccacgccagc 2940 ctcaatgaca ttacagttga tactactgtg aatttagttg ccacctacgg ccacattcaa 3000 accacagctg gtagctggaa taaaactgga acgactgacg taaccgctgg cgacaacctg 3060 tatatacagc tagagcgcgc aggtaatagt tataccgcac gctactcgac tgatggcggt 3120 gccacctata gcaacattgg tggcagctca tttacagaca cccttccaga cacacttaaa 3180 gtgggtttct tcgctacgcc taacaacacc ggtgagcaaa ctttcgttta cgaagatata 3240 caaatcactc agtaa 3255 <210> 117 <211> 391 <212> PRT <213> Microbulbifer degradans <400> 117 Met Gly Met Gly Thr Lys Ile Asn Phe Leu Leu Leu Gly Phe Ile Leu 1 5 10 15 Ser Ala Cys Ser Leu Ser Gly Cys Ala Asp Lys Ile Lys Arg Asn Thr             20 25 30 Pro Leu Thr Glu Thr Ala Leu Pro Ser Lys Lys Ile Leu Tyr Val Gln         35 40 45 Thr Glu Val Cys Asp Pro Pro Ser Gln Leu Thr Gly Ser Cys Tyr Asn     50 55 60 Ser Leu Gln Arg Ala Ile Asp Val Ala His Thr Val Pro Ser Ala Thr 65 70 75 80 His Val Thr Ile Glu Met Ala Ala Gly His Tyr His Glu Arg Ile Val                 85 90 95 Leu Ser Arg Gly Asn Ile Asp Ile Val Gly Ala Gly Lys Asn Lys Thr             100 105 110 Tyr Val Gln Tyr Asn Leu Asn Ala Glu Gln Gly Lys Ala Tyr His Arg         115 120 125 Asp Gly Trp Gly Thr Pro Gly Ser Ala Thr Phe Thr Ile Asn Ala Ser     130 135 140 Glu Val Asn Val Ser Asp Leu Thr Ile Glu Asn Thr Phe Asp Phe Leu 145 150 155 160 Arg Asn Asp Ser Lys Asp Lys Thr Asp Pro Ser Lys Val Arg Ala Ser                 165 170 175 Gln Gly Val Ala Leu Leu Leu Asp Glu His Ser Asp Lys Val Ala Leu             180 185 190 Tyr Arg Val Gly Leu Tyr Gly Tyr Gln Asp Thr Leu Phe Ala Asn Gly         195 200 205 Lys Arg Ala Phe Ile Tyr Gln Ser Asp Ile Ala Gly Asn Val Asp Phe     210 215 220 Ile Phe Gly Ala Gly Gln Val Val Ile Glu Asn Ser Arg Val Ile Ser 225 230 235 240 Arg Pro Arg Gly Lys Ala Ile Ala Ser Asn Glu Ile Ala Gly Tyr Ile                 245 250 255 Thr Ala Pro Ser Thr Asn Ile Thr Asp Ala Phe Gly Leu Val Phe Ile             260 265 270 Asn Ser Arg Leu Glu Arg Glu Gln Gly Val Ala Asp Ala Ser Val Thr         275 280 285 Leu Gly Arg Pro Trp His Pro Thr Thr Asn Phe Ser Asp Gly Arg Tyr     290 295 300 Ala Asp Pro Asn Ala Ile Gly His Ala Leu Phe Phe Asn Cys Phe Met 305 310 315 320 Asp Ala His Ile His Pro Ala Arg Trp Ser Ser Met Lys Gly Thr Ala                 325 330 335 Lys Asp Gly Ser Lys Thr Leu Val Phe Thr Pro Glu Gln Ser Arg Phe             340 345 350 Phe Glu Val Gln Ser Phe Gly Pro Ser Gly Asn Asp Glu Val Thr Thr         355 360 365 Ser Tyr His Ser Leu Ser Ala Asp Ser Leu Arg Glu Gln Ala Leu Gly     370 375 380 Asp Trp Asn Val Ser Ile Asn 385 390 <210> 118 <211> 1176 <212> DNA <213> Microbulbifer degradans <400> 118 gtgggtatgg gtacaaaaat taattttttg ctattaggtt ttattttgtc tgcatgttca 60 ttaagtggct gcgccgacaa aataaagcgc aatacgcctt taaccgaaac ggcattgccg 120 agtaaaaaaa tactctatgt acaaaccgaa gtatgtgacc cgccttcgca attaacgggt 180 agttgctaca acagcttgca gcgagctatt gacgtggcgc acacagtgcc gtctgctacc 240 catgtaacca ttgaaatggc tgcggggcat taccacgaac gcattgtgct tagccgtggc 300 aatatcgata ttgttggtgc aggtaaaaat aaaacctatg ttcaatacaa cctgaatgcc 360 gagcagggta aagcttatca ccgcgacggt tggggtactc ctggctcagc tacatttacc 420 attaatgcca gtgaagtaaa tgttagcgat ttaactatcg aaaatacttt cgacttttta 480 agaaatgatt caaaagataa aaccgaccct tcaaaagtga gggcatcgca aggcgttgca 540 ttattattgg atgaacacag cgataaggtt gcgctgtatc gagtaggcct atacggatac 600 caagacaccc tatttgcaaa tggaaagcgt gcatttatct accaatcaga tattgcaggc 660 aatgttgatt ttatttttgg cgctggccaa gtggttatag aaaatagtcg tgttatttct 720 aggccgcgcg gcaaagccat tgcttccaat gaaattgccg gctatatcac agcgccatcc 780 accaatatta cggacgcctt tggtctggtt tttattaata gtcgattaga acgtgagcaa 840 ggcgtggcag atgcgtcggt caccttgggt cgcccttggc accctacaac caatttcagc 900 gatggccgat atgccgaccc aaacgcgatt ggccatgcgc tattttttaa ctgctttatg 960 gatgcgcata ttcaccccgc gagatggtct agcatgaaag gcaccgctaa agacggcagt 1020 aaaacgctag tgtttacgcc cgagcaatcg cgtttttttg aagttcagtc ctttggcccc 1080 agcggcaacg atgaagtaac cacctcgtat cattcgttaa gcgccgactc attacgcgaa 1140 caagcgctcg gcgattggaa tgtatcaatt aactaa 1176 <210> 119 <211> 914 <212> PRT <213> Microbulbifer degradans <400> 119 Met Ser Ala Leu Thr Arg Pro Lys Phe Gly Ala His Thr Lys Leu Phe 1 5 10 15 His Ala Ile Lys His Ala Leu Thr Pro Val Ile Phe Leu Gly Ala Ala             20 25 30 Ala Phe Pro Leu Ala Ala His Ser Gln Tyr Asn Met Glu Asn Leu Asp         35 40 45 Arg Gly Leu Val Ala Ile Asp Arg Lys Asp Gly Ser Val Leu Val Ser     50 55 60 Trp Arg Trp Leu Gly Gln Glu Pro Asp Asn Thr Ser Phe Asn Val Tyr 65 70 75 80 Arg Asn Gly Thr Leu Leu Thr Ser Ser Pro Leu Thr Asn Lys Thr Asn                 85 90 95 Phe Val Asp Thr Ser Gly Asn Pro Asn Ala Asn Tyr Ala Val Glu Ala             100 105 110 Ile Val Asn Gly Ala Ser Gln Ser Leu Ala Thr Thr His Val Trp Ser         115 120 125 Asp Ile Tyr Arg Thr Ile Pro Leu Gln Arg Pro Pro Gly Gly Thr Thr     130 135 140 Pro Asp Gly Val Ala Tyr Thr Tyr Ser Pro Asn Asp Ile Ser Ala Ala 145 150 155 160 Asp Leu Asp Gly Asp Gly Gly Tyr Glu Leu Ile Val Lys Trp Asp Pro                 165 170 175 Ser Asn Ala Lys Asp Asn Ser Gln Ser Gly Tyr Thr Gly Asn Val Tyr             180 185 190 Leu Asp Ala Tyr Glu Ile Ser Gly Glu Phe Met Trp Arg Ile Asp Leu         195 200 205 Gly Arg Asn Ile Arg Ala Gly Ala His Tyr Thr Gln Phe Leu Ala Phe     210 215 220 Asp Phe Asp Ser Asp Gly Lys Ala Glu Val Ala Val Lys Thr Ala Asp 225 230 235 240 Ala Thr Lys Asp Ser Gln Gly Val Val Ile Gly Asp Ser Asn Ala Asp                 245 250 255 Tyr Arg Asn Ser Ala Gly Tyr Val Leu Ser Gly Pro Glu Tyr Leu Thr             260 265 270 Met Phe Glu Gly Gln Thr Gly Arg Ala Leu Asn Thr Val Asn Tyr Val         275 280 285 Pro Ala Arg Gly Ser Val Ser Ser Trp Gly Asp Asn Tyr Gly Asn Arg     290 295 300 Val Asp Arg Phe Leu Gly Gly Val Ala Tyr Leu Asp Gly Gln Asn Pro 305 310 315 320 Ser Leu Ile Met Ser Arg Gly Tyr Tyr Thr Arg Thr Val Val Ala Ala                 325 330 335 Trp Asp Trp Arg Asn Gly Gln Leu Ser Gln Arg Trp Val Phe Asp Ser             340 345 350 Asn Thr Ser Gly Asn Ser Ser Tyr Ala Gly Gln Gly Ala His Ser Leu         355 360 365 Thr Ile Gly Asp Val Asp Ala Asp Gly Lys Gln Glu Ile Val Phe Gly     370 375 380 Ala Met Thr Ile Asp Asp Asn Gly Thr Gly Leu Asn Asn Thr Arg Leu 385 390 395 400 Gly His Gly Asp Ala Leu His Leu Ser Asp Met Asp Pro Ser Asn Pro                 405 410 415 Gly Leu Glu Val Phe Met Val His Glu Cys Pro Ser Cys Tyr Gly Glu             420 425 430 His Gly Ile Glu Met His Asp Ala Ala Thr Gly Gln Ile Leu Trp Ser         435 440 445 His Pro Gly Asp Tyr Ile Asp Ile Gly Arg Gly Val Ala Met Asp Ile     450 455 460 Asp Pro Arg Tyr Ala Gly Tyr Glu Ala Trp Ala Ser Arg Gly Gly Leu 465 470 475 480 Tyr Ser Ala Lys Gly Glu Thr Ile Ser Ser Thr Arg Pro Ser Gln Ile                 485 490 495 Asn Phe Ala Ala Trp Trp Asp Gly Asp Leu Leu Arg Glu Ile Leu Asp             500 505 510 Asn Asn Tyr Ile Asn Lys Trp Asn Tyr Thr Ala Ser Ser Thr Thr Arg         515 520 525 Leu Leu Ser Ala Gly Asn Tyr Gly Ala Ala Ser Asn Asn Gly Thr Lys     530 535 540 Ala Thr Pro Gly Leu Ser Ala Asp Ile Leu Gly Asp Trp Arg Glu Glu 545 550 555 560 Val Val Trp Arg Asn Ser Asn Asn Gln Glu Leu Met Val Phe Thr Thr                 565 570 575 Pro His Glu Ser Glu Tyr Arg Leu Arg Thr Leu Met His Asp Pro Gln             580 585 590 Tyr Arg Thr Ala Ile Ala Trp Gln Asn Val Gly Tyr Asn Gln Pro Pro         595 600 605 His Pro Ser Tyr Phe Leu Gly Ala Gly Met Thr Thr Pro Asn Gln Pro     610 615 620 His Ile Thr Ile Val Gly Glu Gly Thr Val Gln Pro Pro Ala Pro Thr 625 630 635 640 Gly Asp Ala Ile Gln Glu Asn Ala Thr Gly Phe Cys Gly Tyr Glu Gly                 645 650 655 Thr Ile Asp Ser Asn His Ser Gly Tyr Thr Gly Ala Gly Phe Thr Asn             660 665 670 Thr Thr Asn Ala Thr Gly Ala Gly Ile Asn Trp Asn Leu His Ala Ser         675 680 685 Thr Ala Gly Thr Tyr Arg Leu Ser Met Arg Tyr Ala Asn Gly Ser Thr     690 695 700 Ala Arg Gly Ala Val Leu Asn Val Glu Thr Thr Gly Asn Ser Tyr Pro 705 710 715 720 Met Ala Phe Ala Pro Thr Ser Thr Trp Thr Asn Trp Gln Glu Glu Tyr                 725 730 735 Val Asp Ala His Leu Asn Ala Gly Tyr Asn Ser Ile Arg Leu Glu Ala             740 745 750 Asn Gln Ala Ala Gly Leu Pro Asn Leu Asp Ala Ile Tyr Leu Ala Asp         755 760 765 Gly Leu Thr Ala Ala Ala Cys Gly Gln Thr Ser Ser Ser Ser Ser Ser     770 775 780 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 785 790 795 800 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly Gly Thr Thr Ala                 805 810 815 Gly Leu Ala Cys Ala Asn Gly Ser Thr Asp Thr Trp Gly Thr Gly Phe             820 825 830 Val Leu Asn Gly Phe Gln Val Glu Asn Glu Gly Gln Gln Ala Thr Asn         835 840 845 Asn Trp Gln Val Thr Leu Gln Phe Asp Gln Pro Val Asn Ile Thr Asn     850 855 860 Ala Trp Gly Val Asn Val Glu Thr Thr Gly Thr Thr Val Val Ala Thr 865 870 875 880 Ser Val Gly Tyr Asn Ser His Leu Asn Pro Gly Gln Ser Ala Ser Phe                 885 890 895 Gly Met Gln Gly Thr Ser Ala Thr Ala Val Ser Asn Pro Leu Cys Ser             900 905 910 Ala gln          <210> 120 <211> 82 <212> PRT <213> Microbulbifer degradans <400> 120 Met Ala Cys Trp Pro Ser Phe Ser Thr Trp Lys Pro Phe Asn Thr Asn 1 5 10 15 Pro Val Pro His Val Ser Val Leu Pro Leu Ala Gln Ala Arg Pro Ala             20 25 30 Val Val Pro Pro Asp Glu Leu Leu Leu Glu Leu Leu Leu Glu Leu Leu         35 40 45 Leu Glu Leu Glu Glu Leu Leu Glu Glu Leu Glu Glu Leu Leu Glu Asp     50 55 60 Glu Leu Leu Leu Asp Val Trp Pro His Ala Ala Ala Val Arg Pro Ser 65 70 75 80 Ala arg          <210> 121 <211> 2745 <212> DNA <213> Microbulbifer degradans <400> 121 atgtcggcac tcactcgtcc caaatttggt gcacacacca aactgttcca cgctataaag 60 catgcgttaa cccccgttat atttttaggt gctgctgctt tcccacttgc cgctcacagc 120 caatacaaca tggaaaacct cgaccgcggg ttggtggcca tagatcgcaa agacggcagc 180 gtattagtaa gctggcgctg gttggggcaa gagccagata acaccagctt caacgtatat 240 agaaacggca cactattaac cagctccccc cttaccaata aaacaaactt tgtagatacc 300 agcggcaacc caaacgctaa ttacgcggta gaagccatag taaacggcgc gagccaatca 360 ctagccacca cacatgtatg gagcgatata taccgcacta ttccgctgca aagaccacca 420 ggtggcacca cccccgacgg agtggcatac acctatagcc ccaacgatat aagcgcagcc 480 gatttagacg gcgatggcgg ttacgagcta atcgtaaaat gggacccctc caacgcaaaa 540 gacaactcgc aaagtggcta caccggcaat gtgtatctcg acgcttacga aatatctggc 600 gagtttatgt ggcgcataga cctaggccgc aatattcgag caggcgccca ctacacgcaa 660 tttttagcat tcgattttga tagcgatggc aaagcagaag ttgcagttaa aaccgcagac 720 gccaccaaag acagccaagg agtagtaata ggagacagca atgccgatta ccgcaacagt 780 gcaggctatg ttttatctgg ccccgaatac ctcaccatgt ttgaaggcca aaccggtaga 840 gcgctaaata ccgttaacta cgtacccgcg cgcggcagtg tttctagctg gggcgacaac 900 tacggcaacc gtgttgatag atttttaggt ggcgtagcct acttagatgg ccaaaaccca 960 agtttgatta tgtcgcgcgg gtactacacc cgcaccgtgg tagcagcatg ggactggcgc 1020 aatgggcagc ttagccaacg ctgggtattt gattccaaca ccagtggcaa cagcagctat 1080 gcaggccaag gcgcgcacag ccttaccatt ggcgatgtag atgcagacgg aaaacaagaa 1140 attgtatttg gcgccatgac catagatgac aacggcaccg gcttgaacaa tacccgccta 1200 ggtcacggcg atgcactgca cctatccgac atggacccaa gcaacccagg cttagaagtg 1260 tttatggtgc acgaatgccc ctcttgctat ggcgagcacg gaatagaaat gcacgatgcc 1320 gccaccggcc aaatactatg gagccaccca ggcgactaca tagacatagg ccgaggtgta 1380 gccatggata tagacccacg ttatgccggc tacgaagctt gggcttcgcg cggtggttta 1440 tacagtgcaa aaggcgaaac aatatcgagc acgcgcccgt cgcaaataaa ctttgccgct 1500 tggtgggatg gcgacttact gcgcgaaata ctcgataaca attacataaa caaatggaac 1560 tacaccgcca gctctaccac gcgcttgcta agcgcaggta actacggtgc agcatcgaac 1620 aacggcacca aggctacacc agggctttcg gccgatattc ttggtgactg gcgcgaagaa 1680 gtggtatggc gcaacagcaa caaccaagaa cttatggtgt ttaccacccc acacgaaagt 1740 gaataccgct taagaaccct aatgcacgac ccgcaatacc gcacagccat agcttggcaa 1800 aacgtgggct acaaccaacc acctcacccc tcttactttt tgggtgcggg tatgactacc 1860 cccaaccaac cacacataac catagttggc gaaggcacag tgcagcctcc agcccctaca 1920 ggcgacgcaa tacaagaaaa cgccaccggc ttttgcggct acgaaggcac tatagatagc 1980 aaccactctg gctatacagg cgcgggcttt actaacacta ccaacgcaac aggtgcaggc 2040 attaactgga acctacatgc gtccaccgcc ggtacatacc gcctaagcat gcgttacgcg 2100 aatggcagta cggcacgagg tgctgtgcta aacgtagaaa caaccggtaa tagctacccc 2160 atggcatttg caccaacaag cacatggaca aactggcaag aagaatatgt agacgcccac 2220 ctaaacgccg gctacaacag cattcggctt gaagcaaacc aagcggcagg tttgcccaac 2280 ctcgatgcca tctacctagc cgatggtctt acggcggcag cgtgcggcca aacatctagc 2340 agcagctcgt cctccagtag ctcctctagt tcttccagca gctcttcaag ctctagtagc 2400 agttccagta gtagctcgag cagcagctca tccggcggta caacagcagg cctagcttgc 2460 gccaacggca gtacagatac atggggaacg gggtttgtgt taaacggctt ccaagtagaa 2520 aacgaaggcc agcaagccac caataactgg caagtgacac ttcaattcga ccagcccgta 2580 aacataacca acgcatgggg tgtaaacgta gaaacaacag gcacaaccgt tgtagcaaca 2640 agcgtaggct acaacagcca cctaaacccg gggcaaagtg ctagctttgg aatgcaagga 2700 acatcggcaa cggcggtaag caacccgcta tgcagtgccc agtaa 2745 <210> 122 <211> 789 <212> PRT <213> Microbulbifer degradans <400> 122 Met Arg Ser Leu Ala Pro Ile Lys Ile Arg Glu Lys Ile Arg Glu Thr 1 5 10 15 Leu Met Phe Asn Ile Arg Ala Trp Gln Leu Asp Leu Pro Leu Ala Leu             20 25 30 Leu Ala Phe Ser Ser Thr Ser Tyr Ala Ile Asp Asn Gly Thr Tyr Thr         35 40 45 Ile Gln Ser Lys His Ser Gly Lys Val Val Glu Val Ala Ala Gly Ser     50 55 60 Val Asp Asp Ala Ala Asn Val Ala Gln Trp Pro Ser Asn Gly His Pro 65 70 75 80 Thr Gln Gln Trp Ile Ile Thr Gln Ile Ser Gly Asp Asp Tyr Ser Val                 85 90 95 Ile Asn Val Asn Ser Gly Lys Ala Met Glu Val Tyr Asp Phe Gly Thr             100 105 110 Thr Asp Gly Gly Asn Ile Val Gln Tyr Pro Tyr Trp Gly Gly Ala Pro         115 120 125 Gln Leu Trp Thr Ile Thr Asp Gln Gly Gly Tyr Tyr Ser Leu Ile Asn     130 135 140 Lys His Ser Gly Lys Ala Leu Asp Leu Leu Asn Trp Asp Thr Thr Asp 145 150 155 160 Gly Ala Asn Ile Gly Gln Trp Ala Trp Trp Gly Gly Asp Ala Gln Leu                 165 170 175 Trp Ala Leu Asn Thr Val Gln Pro Ser Thr Val Thr Phe Thr Leu Glu             180 185 190 Glu Asn Gln Ala Gly Phe Cys Ser Val Asp Gly Ser Ile Asp Ser Asn         195 200 205 His Thr Gly Tyr Thr Gly Ser Gly Phe Ala Asn Thr Thr Asn Ala Asn     210 215 220 Gly Gln Gly Val Asn Trp Ser Val Asn Val Ala Thr Ala Gly Thr Tyr 225 230 235 240 Thr Phe Thr Trp Arg Tyr Ala Gly Thr Ser Asn Arg Pro Ala Asn Leu                 245 250 255 Leu Ile Asp Gly Ser Thr Gln Val Ser Gly Ile Ala Leu Asn Ser Thr             260 265 270 Gly Ala Trp Ala Thr Trp Ala Asn Ser Ala Glu Ile Ser Val Trp Leu         275 280 285 Asp Thr Gly Val His Ser Leu Arg Leu Gln Ala Thr Thr Ser Ala Gly     290 295 300 Leu Pro Asn Ile Asp Ser Leu Ser Ile Thr Gly Gln Ser Ala Ala Ala 305 310 315 320 Gly Asn Cys Ser Gly Ala Ile Glu Pro Ile Thr Phe Ala Thr Pro Ser                 325 330 335 Phe Thr Asn Ile Ala Val His Asp Pro Ser Val Ile Glu Ala Asn His             340 345 350 Gln Tyr Tyr Val Phe Gly Ser His Leu Ser Val Ala Lys Thr Pro Asp         355 360 365 Leu Lys Asn Trp Ser Arg Val Ala Asp Gly Val Thr Thr Asn Asn Pro     370 375 380 Leu Phe Asn Asp Val Thr Ser Glu Leu Ala Glu Ala Leu Ala Trp Ala 385 390 395 400 Glu Thr Thr Thr Leu Trp Ala Pro Asp Val Thr Tyr Val Asn Gly Arg                 405 410 415 Tyr Leu Met Tyr Tyr Asn Ala Cys Arg Gly Asp Ser Pro Leu Ser Ala             420 425 430 Met Gly Ile Ala Ser Ser Asn Asn Ile Glu Gly Pro Tyr Thr Asn Asp         435 440 445 Gly Ile Phe Leu Lys Ser Gly Met Trp Gly Gln Thr Ser Glu Asp Gly     450 455 460 Thr Val Tyr Asp Ala Thr Val His Pro Asn Ala Val Asp Pro Val Ile 465 470 475 480 Phe Ser Asp Ala Asn Asn Arg Met Trp Met Thr Tyr Gly Ser Tyr Ser                 485 490 495 Gly Gly Ile Phe Ile Met Glu Leu Asn Pro Ser Thr Gly Phe Pro Tyr             500 505 510 Ala Gly Gln Gly Tyr Gly Lys His Leu Met Gly Gly Asn His Ala Arg         515 520 525 Ile Glu Gly Ala Tyr Thr Ile Tyr Ser Pro Glu Thr Gly Tyr Tyr Tyr     530 535 540 Met Tyr Val Ser Tyr Gly Gly Leu Gly Ala Asp Gly Gly Tyr Asn Val 545 550 555 560 Arg Val Ala Arg Ala Thr Ser Pro Asp Gly Pro Tyr Tyr Asp Ala Asn                 565 570 575 Gly Thr Asn Met Ala Asn Val Lys Ser Asn Pro Ser Leu Pro Leu Phe             580 585 590 Asp Asp Ala Ser Ile Ala Pro His Gly Val Lys Leu Met Gly Asn His         595 600 605 Val Phe Ser Gly Thr Asn Asn Val Leu Gly Tyr Val Ser Pro Gly His     610 615 620 Asn Ser Ala Tyr Arg Asp Ala Thr Thr Gly Gln Thr Phe Leu Leu Phe 625 630 635 640 His Thr Arg Phe Pro Gly Arg Gly Glu Glu His Glu Val Arg Val His                 645 650 655 Glu Val Phe Tyr Asn Asp Ala Gly Trp Pro Val Ile Ala Pro Leu Arg             660 665 670 Tyr Ala Gln Lys Val Asp Ala Asn Asn Pro Asn Arg Ser Ala Ser Glu         675 680 685 Leu Asn Ala Val Tyr Ala Ser Glu Leu Pro Gly Ser Tyr Gln Leu Ile     690 695 700 Asn His Gly Lys Asp Ile Ser Ala Thr Ile Lys Asn Ser Val Asn Ile 705 710 715 720 Thr Leu Asn Ser Asn Gly Ser Ile Ser Gly Glu Leu Ser Gly Ser Trp                 725 730 735 Thr Tyr Asn Ala Asn Thr Arg Asn Thr Val Ile Thr Val Ala Gly Val             740 745 750 Ala Tyr Arg Gly Val Val Ser Arg Gln Trp Asn Gln Ala Arg Asn Arg         755 760 765 Phe Glu Val Thr Phe Ser Ala Leu Ser Ala Asp Gly Thr Ala Ile Trp     770 775 780 Gly Val Asn Ser Asp 785 <210> 123 <211> 2370 <212> DNA <213> Microbulbifer degradans <400> 123 gtgcgcagtc tagcccccat aaaaataaga gagaaaataa gagagacact catgtttaat 60 atacgcgctt ggcaacttga cttgcccttg gcgttattgg cgttttcgtc tacaagttac 120 gctatcgata acggcactta cacaattcaa tctaaacaca gtggcaaggt tgtagaagtg 180 gccgcaggca gtgtagatga tgctgcaaat gtggcccaat ggcccagtaa tggccatcct 240 acccagcagt ggataattac ccaaattagc ggcgatgatt actcggtaat aaatgtaaat 300 agcggcaaag ctatggaggt atacgacttc ggcaccacgg acggcggcaa catagtgcaa 360 tacccctact ggggaggcgc cccccagttg tggacaatta cagatcaagg cggttattac 420 agcttaataa acaaacacag tggtaaagct ttagatttgt taaattggga taccacagac 480 ggcgccaata taggccagtg ggcttggtgg ggcggcgatg cgcaactgtg ggcactgaac 540 acagtgcaac ccagcacagt caccttcaca cttgaagaaa accaagcggg tttttgcagc 600 gtagatggca gcatagatag caatcatacg ggctataccg gcagcgggtt tgccaataca 660 accaatgcaa atggccaagg agttaattgg tcggtaaatg tagccacagc tggtacctat 720 acgtttacat ggcgctatgc gggcactagc aaccgcccag ccaacttgct aatagatggc 780 agcacacagg tttcgggcat tgctttaaat tcaaccggcg catgggcaac ttgggctaat 840 agtgcagaaa taagtgtttg gcttgacaca ggtgtgcact cacttcggct gcaagccaca 900 accagcgcgg gcttacctaa tatagattcg cttagcataa ccggccaaag cgcagcagcg 960 ggcaactgca gcggcgcaat tgaacctatc acttttgcca caccaagctt taccaatata 1020 gcggtgcacg acccctcggt aatagaagca aaccatcaat actacgtatt tggctcccac 1080 ctttctgtgg ctaaaacgcc cgacctaaaa aactggtcgc gcgtggcgga tggtgtaacc 1140 accaataacc cattgtttaa cgatgtaacc agcgaacttg cagaagcatt agcttgggca 1200 gaaaccacta ccctgtgggc gccagatgtt acctatgtaa atggtcggta tttgatgtat 1260 tacaacgcct gccgtggcga ctcaccactg tcggctatgg gtattgcttc ttcgaacaac 1320 atagaaggcc cttacactaa cgatggtata ttccttaaat ctggaatgtg gggccaaacc 1380 agcgaagatg gcactgtgta cgacgcaacc gtgcacccca atgctgtgga ccccgttatt 1440 tttagcgacg caaataatcg catgtggatg acctacggtt cgtattcggg tggtattttt 1500 attatggagt taaacccatc cacggggttc ccttacgcgg ggcaaggtta tggtaaacat 1560 ttaatgggtg gcaaccacgc gcgcattgaa ggcgcttaca ccatctacag cccagaaacg 1620 ggctactact atatgtatgt aagctacggt ggcctaggcg cagatggcgg ctataacgtt 1680 cgtgtggccc gagcaactag cccagacggc ccctactatg atgccaacgg caccaatatg 1740 gccaacgtaa aaagcaaccc aagcttgcca ctgttcgacg acgccagcat agcaccccac 1800 ggtgtaaaac ttatgggtaa ccacgtgttt agcggcacta acaatgtact tggttacgta 1860 tcaccagggc acaactctgc ataccgtgac gccactaccg gccaaacatt tttactattc 1920 cacacacgct tccctgggcg cggcgaagag catgaagtgc gagtgcatga agtgttctac 1980 aacgatgcag gctggccggt aatagcacca ttgcgctatg cccaaaaagt agatgccaac 2040 aacccaaata gaagtgcgag cgagctaaat gcagtgtacg caagcgaact gccaggtagc 2100 tatcagctaa ttaaccacgg caaagacata agcgcgacaa ttaaaaattc cgttaacatc 2160 acgctaaaca gcaacggcag tatctctggc gagctatctg gcagctggac atacaacgcc 2220 aacacccgca ataccgtaat caccgttgcc ggtgtggcct accgcggcgt ggtatctcgc 2280 caatggaacc aagcacgcaa ccgcttcgaa gtcaccttca gcgccctatc tgcagacggc 2340 acagcaatat ggggggtgaa cagcgactaa 2370 <210> 124 <211> 362 <212> PRT <213> Microbulbifer degradans <400> 124 Met Ser Ser Phe Ile Met Asp Lys Ser Gln Leu Gln Ser Gly Phe Ala 1 5 10 15 Phe Lys Thr Ser Gly Phe Asn Val Leu Ile Val Val Thr Phe Leu Ala             20 25 30 Leu Leu Ala Ala Leu Val Gly Cys Ser Ser Ala Lys Leu Ala Pro Val         35 40 45 Ala Ser Pro Ser Leu Pro Gln Pro Leu Val Ala Gln Arg Ala Asp Pro     50 55 60 Trp Val His Lys His Ser Asp Gly Tyr Tyr Tyr Phe Ile Ala Thr Val 65 70 75 80 Pro Ala Tyr Asp Arg Leu Glu Met Arg Arg Ala Thr Thr Ile Ala Gly                 85 90 95 Leu Arg Ser Ala Pro Ala Val Val Val Trp Gln Arg Asn Thr Ile Gly             100 105 110 Gly Met Ser Ala Asn Ile Trp Ala Pro Glu Leu His Phe Ile Asp Gly         115 120 125 Lys Trp Tyr Ile Tyr Val Ala Ala Ala Thr Asp His Asn Lys Pro Trp     130 135 140 Thr Ile Arg Met His Thr Leu Ser Asn Ala Ser Ala Asn Pro Met Gln 145 150 155 160 Gly Glu Trp Gln Glu Glu Gly Arg Phe His Thr Pro Leu Asp Thr Phe                 165 170 175 Ser Leu Asp Ala Thr Thr Phe Glu His Arg Gly Lys Arg Tyr Leu Val             180 185 190 Trp Ala Gln Gln Asn Glu Ala Arg Thr Tyr Asn Ser Ala Leu Leu Ile         195 200 205 Ala Gln Met Asp Ser Pro Thr Ser Ile Thr Gly Pro Ile Val Thr Leu     210 215 220 Ser Glu Pro Thr Leu Pro Trp Glu Ile Gly His Lys Val Asn Glu 225 230 235 240 Gly Ala Ala Val Ile Lys His Gly Lys Arg Ile Phe Ile Ser Tyr Ser                 245 250 255 Ala Ser Ala Thr Asp His Asn Tyr Ala Met Gly Leu Leu Trp Ala Asp             260 265 270 Glu Asn Ala Asp Leu Leu Asp Ala Ala Ser Trp Thr Lys Ser Pro Glu         275 280 285 Pro Val Phe Tyr Ser Asn Glu Gln Leu Lys Arg Phe Gly Pro Gly His     290 295 300 Asn Cys Phe Val Lys Ala Glu Asp Gly Val Thr Asp Leu Met Val Tyr 305 310 315 320 His Ala Arg Asp Tyr Lys Glu Ile Asp Gly Glu Pro Leu Arg Asp Pro                 325 330 335 Asn Arg His Thr Arg Val Arg Lys Val Tyr Trp Asp Glu Gln Gly Met             340 345 350 Pro Asp Phe Arg Gln His Glu Ala Asp Leu         355 360 <210> 125 <211> 1089 <212> DNA <213> Microbulbifer degradans <400> 125 atgagtagct tcataatgga taaaagccag ctacaaagtg gttttgcgtt taaaacaagt 60 ggttttaacg tgctgattgt tgtcacattt ttggcattgc ttgcagcgct tgttggctgc 120 agcagcgcca agctcgcacc cgtcgcctct cctagtttac cgcagccatt agtggcccaa 180 cgggcagacc cttgggtgca caagcacagc gatggttatt actactttat agcaacggta 240 ccagcatacg accgcttaga aatgcgtagg gccacaacca tagcaggctt acgtagcgcg 300 cccgctgtag tggtatggca gcgcaatact attggaggta tgagcgcgaa tatttgggcg 360 cccgagctgc attttattga tggtaaatgg tacatctatg tagcggctgc caccgatcac 420 aacaagccgt ggacaattcg tatgcacacg ctttccaatg catcggccaa ccctatgcaa 480 ggtgagtggc aagaagaggg gcgctttcat acaccgctag atactttctc gctagatgcc 540 acaacctttg agcacagggg taaacgctat ttagtatggg cgcaacagaa tgaagcccgt 600 acttataact cggcgttact tatagcgcaa atggatagcc ctacaagtat tactggcccc 660 attgttacct taagtgaacc gacattaccg tgggaaatta ttggccataa ggttaatgag 720 ggtgcggcag taattaaaca cggtaagcgt atttttataa gttattccgc cagtgcgacc 780 gatcataact atgcgatggg tttgttatgg gcagacgaaa acgctgattt gctcgacgca 840 gcaagctgga ccaagtcacc cgagcctgta ttttactcaa acgaacaatt aaagcgcttt 900 ggccctggcc ataattgttt tgttaaagct gaagatggtg ttaccgattt aatggtgtac 960 cacgcgcgtg attataaaga gatagatggt gagccattgc gagacccaaa ccgccacacg 1020 cgggtgcgca aagtgtattg ggatgaacag ggcatgccgg attttcgtca acatgaagca 1080 gacctatag 1089 <210> 126 <211> 314 <212> PRT <213> Microbulbifer degradans <400> 126 Met Lys Lys Leu Ser Pro Leu Ile Glu Gln Arg Ala Asp Pro Tyr Ile 1 5 10 15 Tyr Lys His Thr Asp Gly Tyr Tyr Tyr Phe Thr Ala Ser Val Pro Ala             20 25 30 Tyr Asp Gly Ile Glu Leu Arg Arg Ala Lys Thr Ile Gln Ala Leu Ala         35 40 45 Thr Ala Glu Thr Val Met Val Trp Arg Lys Pro Ser Glu Gly Asp Tyr     50 55 60 Ser Glu Leu Ile Trp Ala Pro Glu Ile His Phe Asn Met Gly Ala Trp 65 70 75 80 Tyr Val Tyr Phe Ala Ala Ala Pro Ser Arg Glu Ile Lys Phe Asp Leu                 85 90 95 Phe Gln His Arg Met Tyr Ala Ile Ser Cys Ser Asp Ala Asn Pro Leu             100 105 110 Thr Gly Glu Trp Ile Phe Glu Gly Lys Ile Asp Ser Gly Ile Asp Ala         115 120 125 Phe Cys Leu Asp Ala Thr Thr Phe Thr His Ser Asn Glu Leu Tyr Tyr     130 135 140 Val Trp Ala Gln Lys Glu Leu Asp Val Arg Gly Asn Ser Asn Leu Met 145 150 155 160 Ile Ala Lys Met Glu Thr Pro Thr Lys Leu Ala Thr Lys Pro Val Arg                 165 170 175 Leu Ser Lys Pro Glu Tyr Asp Trp Glu Ile Gln Gly Phe Trp Val Asn             180 185 190 Glu Gly Pro Ser Ile Val Lys His Gly Ser Arg Ile Phe Ile Ser Tyr         195 200 205 Ser Gly Ser Ala Thr Asp Glu Arg Tyr Ala Met Gly Ile Leu Trp Ala     210 215 220 Glu Gln Ser Ala Asp Leu Leu Asp Pro Ala Ser Trp Thr Lys Ser Val 225 230 235 240 Glu Pro Val Leu Val Ser Glu Pro Ser Glu Lys Val Phe Gly Pro Gly                 245 250 255 His Asn Ser Phe Thr Val Asp Glu Glu Gly Asn Asp Met Leu Val Tyr             260 265 270 His Ala Arg Asn Tyr Thr Glu Ile Glu Gly Asp Pro Leu Trp Asp Pro         275 280 285 Asn Arg His Thr Tyr Val Lys Lys Leu Arg Trp Asp Glu Thr Gly Met     290 295 300 Pro Ile Phe Gly Ser Pro Ala Phe Glu Glu 305 310 <210> 127 <211> 945 <212> DNA <213> Microbulbifer degradans <400> 127 gtgaaaaaat tatcgcctct tatagagcaa cgtgcagacc cttatattta taaacacacc 60 gacggctact attattttac ggcttcggtg cccgcctatg atggcataga actgcgtcgc 120 gcaaaaacta tacaagcgtt agccaccgca gaaaccgtta tggtgtggcg caagccaagc 180 gaaggtgatt atagtgagct tatttgggcg ccagaaatac actttaatat gggggcttgg 240 tatgtatatt ttgctgcggc tccatcacgt gaaattaagt tcgatttatt tcaacaccgc 300 atgtatgcca ttagctgtag cgatgccaac ccgctaacag gtgaatggat atttgaaggt 360 aaaatagata gcggcataga tgcattctgt ttagatgcca ccacctttac tcacagcaat 420 gagctctact atgtttgggc gcaaaaagaa ttagatgttc gcggcaactc taatttgatg 480 atcgcaaaaa tggaaacgcc caccaagctt gccaccaagc ccgtgcgttt atctaaaccc 540 gaatacgact gggagattca gggtttttgg gttaacgaag gcccatccat tgttaagcac 600 ggctcacgta tttttatttc ttattctggc tctgctaccg atgagcgcta cgcaatgggt 660 attttgtggg cagaacaaag cgcagactta ctagacccag caagttggac caagtcggta 720 gagcctgtat tagtttctga accctctgaa aaagtatttg gcccaggcca caatagtttt 780 actgtggatg aagagggtaa cgatatgttg gtgtatcatg ctcgcaatta taccgaaatt 840 gaaggcgacc cgctgtggga cccaaatcgt catacttacg ttaaaaaatt gcgctgggat 900 gaaacaggca tgcctatttt tggcagccct gcgtttgaag agtag 945 <210> 128 <211> 350 <212> PRT <213> Microbulbifer degradans <400> 128 Asn Gly Tyr Tyr Ala Val Leu Asn Lys His Ser Gly Lys Ala Leu Asp 1 5 10 15 Leu Tyr Gly Phe Asp Thr Ser Asn Gly Ala Asn Ile Ala Gln Trp Ala             20 25 30 Phe Trp Gly Gly Asp Pro Gln Gln Trp Gln Phe Thr Lys Ile Ala Asn         35 40 45 Val Gly Ala Pro Pro Val Asp Thr Ser Thr Thr Asn Gly Ala Thr Asn     50 55 60 His Trp Ser Leu Thr Gly Asn Leu Val Thr His Asp Pro Thr Met Ala 65 70 75 80 Tyr Glu Asn Gly Ser Trp Trp Leu Tyr Gln Thr Gly Glu Gly Ile Tyr                 85 90 95 Gly Lys Tyr Ser Ala Asn Gly Leu Ala Trp Asp Gly Leu Pro Ser Val             100 105 110 Phe Pro Asn Gly Leu Ser Trp Trp Lys Thr Tyr Val Pro Gly Gln Ser         115 120 125 Asn Asn Asp Val Trp Ala Pro Asp Val Arg Thr Tyr Asn Gly Arg Val     130 135 140 Tyr Leu Tyr Tyr Ser Ile Ser Thr Phe Gly Ser Arg Val Ser Ala Ile 145 150 155 160 Gly Leu Ala Ser Ala Ser Ser Leu Ala Ala Ser Asp Trp Gln Asp His                 165 170 175 Gly Leu Val Ile Asn Thr Thr Ser Ser Ser Asp Trp Asn Ala Ile Asp             180 185 190 Pro Asp Leu Val Val Asp Glu His Gly Asn Pro Trp Leu Thr Met Gly         195 200 205 Ser Trp Asn Ser Gly Ile Lys Val Met Arg Leu Asn Pro Ile Thr Met     210 215 220 Lys Pro Ile Gly Thr Leu Tyr Ser Ile Ala Gln Lys Gly Gly Gly Ile 225 230 235 240 Glu Ala Pro Ser Ile Val Tyr Arg Arg Gly Tyr Tyr Tyr Leu Phe Val                 245 250 255 Ser Ile Gly Lys Cys Cys Ala Gly Val Asp Ser Thr Tyr Gln Ile Ala             260 265 270 Tyr Gly Arg Ser Thr Ser Ile Thr Gly Pro Tyr Leu Asp Lys Asn Gly         275 280 285 Asn Asp Met Met Ser Gly Gly Gly Ser Ile Leu Asp Ala Gly Asn Asn     290 295 300 Val Trp Val Gly Pro Gly Gly Gln Asp Ile Ile Asn Thr Asp Val Ile 305 310 315 320 Val Arg His Ala Tyr Asp Ala Thr Asp Ala Gly Thr Pro Lys Met Ile                 325 330 335 Ile Ser Thr Leu Asn Trp Asp Ala Asn Gly Trp Pro Lys Tyr             340 345 350 <210> 129 <211> 1053 <212> DNA <213> Microbulbifer degradans <400> 129 aatggctatt atgccgtgct aaataaacac agcggcaaag cgttagattt gtatggtttt 60 gatacgtcta acggcgcgaa tattgcgcaa tgggcctttt ggggcgggga cccgcagcag 120 tggcaattta ccaaaatcgc caatgtaggt gcgccgccag tagatacatc taccaccaac 180 ggtgcaacca accactggtc cttaaccggt aatctagtga ctcacgaccc cacaatggcc 240 tacgaaaacg gctcatggtg gttgtatcaa accggcgagg gaatttacgg taagtattca 300 gccaatggtt tggcgtggga tggcttacct tctgtgtttc ccaatggttt aagttggtgg 360 aagacctatg tacccggcca gtcgaacaac gatgtatggg cgcctgatgt acgcacttat 420 aatgggcggg tttatttgta ctattccatc tctacttttg gctcgcgtgt atctgccatt 480 ggtttggcgt cggcatcgag tttggctgcg agtgattggc aggaccacgg cttagtaatt 540 aataccacct catctagcga ttggaatgcg atcgacccag atttagtggt cgatgagcat 600 ggcaaccctt ggttaacaat gggaagttgg aacagcggta ttaaagtgat gcgcttgaac 660 cccattacca tgaagccaat tggcacactt tattctattg cgcaaaaggg cggcggtatt 720 gaagcgcctt ctattgtgta tcgccgtggg tattactatt tatttgtttc tatcggcaaa 780 tgctgtgcgg gcgtagatag cacctatcaa attgcttacg ggcgctctac aagtattacc 840 ggcccttatt tggataagaa cggcaacgat atgatgagtg gtggtggcag tattttagat 900 gcgggcaaca acgtgtgggt tggccctggt gggcaagata ttattaacac cgatgtcatt 960 gtgcgccacg cgtacgatgc cacagatgca ggcacaccta agatgattat tagtaccttg 1020 aattgggatg ctaatggatg gccgaaatac tag 1053 <210> 130 <211> 346 <212> PRT <213> Microbulbifer degradans <400> 130 Met Leu Asn Lys Asn Lys Arg Pro Ile Thr Phe Ala Leu Val Val Ser 1 5 10 15 Leu Leu Ala Leu Leu Ala Leu Ala Gly Cys Ser Glu Ala Lys Gln Val             20 25 30 Ser Ile His Asp Pro Val Met Ile Lys Glu Gly Asp Thr Tyr Tyr Leu         35 40 45 Phe Ser Thr Gly Pro Gly Ile Thr Met Tyr Ser Ser Ser Asp Met Lys     50 55 60 Asn Trp Arg Arg Glu Gly Glu Val Phe Asn Gln Ala Pro Ser Trp Ala 65 70 75 80 Ser Asn Ala Val Pro Tyr Phe Lys Gly His Leu Trp Ala Pro Asp Ile                 85 90 95 Ile Glu Lys Asp Gly Leu Phe Tyr Leu Tyr Tyr Ser Val Ser Ala Phe             100 105 110 Gly Lys Asn Thr Ser Gly Ile Gly Val Thr Val Ser Pro Thr Leu Asn         115 120 125 Pro Arg Ala Pro Asn Tyr Gly Trp Gln Asp Lys Gly Met Val Leu Arg     130 135 140 Ser Val Pro Glu Arg Asp Glu Trp Asn Ala Ile Asp Pro Asn Ile Val 145 150 155 160 Val Asp Asn Asn Gly Thr Ala Trp Met Ala Phe Gly Ser Phe Trp Gln                 165 170 175 Ser Leu Lys Met Val Ala Leu Asp Ser Ser Trp Thr Lys Ile Ala Glu             180 185 190 Pro Gln Gln Trp His Thr Ile Ala Ala Leu Pro Lys Gly Ser Met Pro         195 200 205 Thr Gly Asp Ala Val Lys Asp Gly Glu Ile Glu Ala Pro Phe Ile Phe     210 215 220 Lys Lys Asn Asp Asp Tyr Phe Leu Phe Val Ser Trp Gly Lys Cys Cys 225 230 235 240 Arg Lys Asp Glu Ser Thr Tyr Arg Leu Ala Met Gly Arg Ser Lys Asn                 245 250 255 Thr Thr Gly Pro Phe Leu Asp Lys Asn Gly Lys Asp Leu Ala Gln Gly             260 265 270 Gly Gly Thr Leu Leu Ile Ser Gly Asn Lys Asn Trp Pro Gly Leu Gly         275 280 285 His Asn Ser Ala Tyr Thr Phe Asp Gly Lys Asp Trp Leu Val Leu His     290 295 300 Ala Tyr Glu Ser Ala Asp Asn Gly Leu Gln Lys Leu Lys Ile Leu Glu 305 310 315 320 Ile Asn Trp Asp Lys Asp Gly Trp Pro Thr Val Asp Thr Lys Glu Leu                 325 330 335 Asp Glu Phe Val Ser Ile Glu Leu Thr Gln             340 345 <210> 131 <211> 1041 <212> DNA <213> Microbulbifer degradans <400> 131 atgcttaaca aaaacaaacg cccaattaca ttcgctttag tcgttagcct cttagccctg 60 cttgcccttg caggctgcag cgaggcaaaa caagtaagca tccacgaccc agtaatgatt 120 aaagaaggtg acacctacta cttgtttagc actggccccg gcataacaat gtatagctct 180 agcgatatga aaaactggcg ccgcgaaggc gaagtattta atcaagcccc tagttgggcc 240 tccaacgccg taccctattt taaaggccac ctgtgggcac ccgacatcat tgaaaaagat 300 ggtctgtttt acctctacta ttctgtgtct gcttttggaa agaacacatc cggcattggc 360 gttaccgtat cgcccacgct taacccacgc gcgcccaatt acggttggca agataaaggc 420 atggtattgc gcagcgtgcc tgagcgcgac gagtggaacg ctatcgaccc caatattgtg 480 gtagataaca acggcaccgc atggatggct tttggctcct tttggcaaag cttaaaaatg 540 gtggcactag acagcagctg gacaaaaata gctgagcctc aacagtggca taccatagca 600 gccttaccca aaggcagtat gcccacaggc gacgcagtaa aggacggcga aatagaagct 660 ccttttattt ttaaaaagaa cgacgattac tttttgtttg taagttgggg taaatgctgc 720 cgcaaagatg aaagcaccta ccgcctagca atgggccgca gcaaaaatac taccggtcca 780 ttcttagata aaaacggcaa agacctcgcc caaggtggtg gcaccctatt aataagtggc 840 aacaaaaact ggcccggctt aggccacaac agcgcctaca ccttcgacgg caaagattgg 900 cttgtgctac acgcctatga atctgcagat aacggtttac aaaaactaaa aatattagaa 960 ataaactggg ataaagacgg ctggccaact gtagatacca aagaactgga tgagtttgtt 1020 agtattgaat taactcaata a 1041 <210> 132 <211> 665 <212> PRT <213> Microbulbifer degradans <400> 132 Met Asp Asn Ile Met Lys Met Ile Lys Leu Ala Leu Ala Val Thr 1 5 10 15 Leu Ala Val Trp Val Ala Gly Cys Thr Asn Gln Ala Gly Leu Asn Ala             20 25 30 Glu Asn Lys Asn Ile Glu Arg Gln Thr Ile Asn Ser Pro Asp Lys Ser         35 40 45 Leu Lys Val Arg Leu Ser Leu Asp Glu Ser Gly Lys Val Phe Tyr Ser     50 55 60 Ile Ser Arg Asn Gly Glu Gln Val Met Leu Pro Ser Gln Leu Gly Val 65 70 75 80 Glu Leu Asn Ser Gln Ala Phe Thr Asp Gly Leu Thr Ile Thr Asp Val                 85 90 95 Asp Ala Gly Lys Val Asn Asp Ser Tyr Thr Leu Leu His Gly Lys Gln             100 105 110 Arg Asp Ile Thr Tyr Asn Ala Asn Glu Lys Ile Tyr Ser Leu Lys Asn         115 120 125 Lys Gln Gly Asp Lys Leu Ile Ile Ala Phe Arg Val Ser Asn Asp Gly     130 135 140 Val Ala Phe Gln Tyr Arg Phe Pro Asn Thr Ala Lys Gln Leu Leu Ala 145 150 155 160 Val Lys Lys Glu Ile Thr Ser Phe Ala Phe Glu His Thr Thr Lys Ala                 165 170 175 Trp Leu Gln Pro Ile Ala Val Ala Gln Thr Gly Trp Ala Asn Thr Asn             180 185 190 Pro Ser Tyr Glu Glu His Tyr Gln Met Asn Ile Pro Val Asp Thr Val         195 200 205 Ser Pro Ser Pro Ala Gly Trp Val Phe Pro Ala Leu Phe Lys Ala Asn     210 215 220 Lys His Trp Leu Leu Ile Thr Glu Ala Gly Met Asn Gly Asp Tyr His 225 230 235 240 Ala Ser Arg Leu His Ala Glu Ser Pro Asn Gly Glu Tyr Ser Leu Gly                 245 250 255 Ile Pro Met Ala Ala Glu Val Phe Glu Gln Asp Gly Asn Lys Gly Ala             260 265 270 Leu Leu Ala Gln Ser Asn Thr Ala Phe His Ser Pro Trp Arg Val Ile         275 280 285 Leu Val Gly Gly Leu Asp Thr Ile Ile Ala Ser Thr Leu Gly Thr Asp     290 295 300 Leu Ala Asp Pro Ala Ile Ala Lys Met Asp Phe Val Lys Pro Gly Thr 305 310 315 320 Ala Ser Trp Ser Trp Ala Leu Leu Lys Asp Glu Ser Val Asn Tyr Glu                 325 330 335 Thr Ser Leu Glu Phe Ile Asp Tyr Ala Ala Glu Met Gly Trp Asp Tyr             340 345 350 Thr Leu Val Asp Ala Asp Trp Asp Arg Arg Ile Gly Tyr Glu Arg Thr         355 360 365 Ala Gln Leu Ala Ala Tyr Ala Gln Ser Lys Asn Val Gly Leu Leu Val     370 375 380 Trp Tyr Asn Ser Ser Gly Asp Trp Asn Thr Thr Glu Tyr Ser Pro Lys 385 390 395 400 Ser Ala Leu Leu Asp Arg Asp Lys Arg Arg Ala Glu Phe Ala Arg Leu                 405 410 415 Gln Asn Met Gly Val Lys Gly Val Lys Ile Asp Phe Phe Pro Gly Asp             420 425 430 Gly Lys Ser Val Met Ala Tyr Tyr Asn Asp Leu Ala Lys Asp Ala Ala         435 440 445 Asp Tyr Asn Leu Leu Val Asn Tyr His Gly Ser Ser Leu Pro Arg Gly     450 455 460 Leu His Arg Thr Tyr Pro Asn Ile Met Thr Met Glu Ser Val His Gly 465 470 475 480 Phe Glu Met Ile Thr Phe Met Gln Pro Ser Ala Asp Lys Ala Ala Thr                 485 490 495 His Met Ala Ile Leu Pro Phe Thr Arg Asn Ala Phe Asp Pro Met Asp             500 505 510 Phe Thr Pro Thr Thr Phe Ser Asp Ile Pro Asn Ile Glu Arg Arg Thr         515 520 525 Ser Asn Gly Phe Glu Leu Ala Leu Pro Val Leu Phe Leu Ser Gly Leu     530 535 540 Gln His Ile Ala Glu Thr Ala Gln Gly Met Ala Thr Asn Ala Pro Asp 545 550 555 560 Tyr Val Lys Ala Tyr Met Arg Asp Ile Pro Val Leu Trp Asp Glu Ser                 565 570 575 Lys Leu Ile Asp Gly Met Pro Gly Glu His Val Val Ile Ala Arg Lys             580 585 590 His Gly Glu Arg Trp Phe Val Ala Gly Ile Asn Ala Thr Asn Glu Ala         595 600 605 Ile Asn Leu Glu Met Asn Phe Asp Phe Ala Leu Gly Lys Gln Gly Thr     610 615 620 Leu Ile Thr Asp Ser Asn Ile Asn Thr Lys Gly Val Glu Ser Phe Thr 625 630 635 640 Ser His Thr Ile Thr Ala Thr Lys Asn Asn Ala Leu Thr Val Lys Ala                 645 650 655 Asn Gly Gly Phe Val Ile Val Phe Asn             660 665 <210> 133 <211> 619 <212> PRT <213> Microbulbifer degradans <133> 133 Met Ala Ala Gly Gln Ile Ile Ser Leu Glu Val Lys Val Lys Lys Ile 1 5 10 15 Glu Glu Ile Met Lys His Thr Ala Arg Thr Ile Ala Leu Gly Ala Thr             20 25 30 Gly Ala Ala Leu Leu Thr Gly Leu Ile Ala Cys Asn Gly Thr Asn Val         35 40 45 Asn Thr Asn Gly Asp Thr Gln Gln Ala Ser Ile Lys Lys Ala Pro Glu     50 55 60 Gly Met Phe Ala Asn Pro Leu Phe Ala Asn Gly Ala Asp Pro Trp Leu 65 70 75 80 Glu Tyr Tyr Asp Gly Asn Tyr Tyr Leu Thr Thr Thr Thr Thr Trp Thr Ser                 85 90 95 Gln Leu Val Met Arg Lys Ser Pro Thr Leu Asp Gly Leu Ser Thr Ala             100 105 110 Leu Pro Val Asn Val Trp Ser Asp Ser Asp Leu Thr Arg Cys Cys Asn         115 120 125 Phe Trp Ala Phe Glu Phe His Arg Leu Asn Gly Pro Asn Gly Trp Arg     130 135 140 Trp Tyr Leu Met Tyr Thr Ser Gly Gln His Gly Thr Leu Asp His Gln 145 150 155 160 His Leu Ser Val Leu Glu Ser Val Gly Asp Asp Pro Met Gly Pro Tyr                 165 170 175 Thr Tyr Lys Gly Glu Met Met Pro Asn Thr Trp Asn Ile Asp Gly Ser             180 185 190 Tyr Leu Glu His Asn Gly Gln Leu Tyr Leu Leu Trp Ser Glu Trp Val         195 200 205 Gly Asp Glu Gln Gln Asn Phe Ile Ser Lys Met Thr Thr Pro Trp Ser     210 215 220 Ile Glu Gly Pro Arg Ala Leu Leu Thr Arg Pro Glu Ala Glu Trp Glu 225 230 235 240 Lys Ser Gly Arg Lys Val Asn Glu Gly Pro Glu Ile Leu Lys Lys Asp                 245 250 255 Gly Arg Thr Phe Leu Ile Tyr Ser Ala Ser Tyr Cys Asp Thr Pro Asp             260 265 270 Tyr Lys Leu Ala Met Lys Glu Leu Thr Gly Asp Asp Pro Met Asn Ser         275 280 285 Glu His Trp Thr Lys Tyr Asp Lys Pro Val Phe Glu Arg Gly Asn Gly     290 295 300 Val Phe Ala Pro Gly His Asn Gly Phe Phe Lys Ser Pro Asp Gly Thr 305 310 315 320 Glu Asp Trp Ile Val Tyr His Gly Asn Ser Lys Glu Glu His Gly Cys                 325 330 335 Gly Ala Thr Arg Ser Val Arg Ala Gln Lys Phe Thr Trp Asn Thr Asp             340 345 350 Gly Thr Pro Asn Phe Gly Glu Pro Ile Pro Glu Gly Gln Phe Leu Pro         355 360 365 Leu Pro Ser Gly Glu Asn Gly Pro Leu Val Thr Ala Leu Gln Gly Ala     370 375 380 Arg Ile Gln Leu Arg Asn Gly Glu Ser Cys Leu Leu Ala Glu Gly Lys 385 390 395 400 Glu Leu Lys Gln Gly Ser Cys Gln Ala Glu Ala Ser Leu Trp Val Met                 405 410 415 Asp Asn Thr Ala Asp Asn His Tyr Arg Phe Gly Asn Val Ala Ser Asn             420 425 430 Leu Phe Leu Thr Ala Asp Glu Gly Leu Ser Gln Ser Ala Trp Val Asn         435 440 445 Thr Ala Ser Gln Arg Trp Ala Leu Asn Ala Gly Glu Gly Asn Phe Val     450 455 460 Ala Phe Thr Asn Lys Tyr Thr Gly Asp Ala Leu Met Gln Asn Asn Trp 465 470 475 480 Gln Ile Leu Pro Val Gly Lys Val Ala Ile Ser Ser Ile Gln Ser Gly                 485 490 495 Arg Val Leu Gln Ala Cys Asp Lys Asn Ser Ala Asn Val Asn Gln Gly             500 505 510 Gly Trp Gln Gly Arg Ala Cys Gln Ala Trp Gln Phe Asn Pro Ala Ser         515 520 525 Glu Gly His Val Gln Ile Lys Thr Gly Asn Gln Cys Leu Thr Val Glu     530 535 540 Asn Lys Ser Ile Val Pro Gly Thr Asn Val Ile Ala Gly Glu Cys Glu 545 550 555 560 Ser Thr Ser Ser Gln Trp Leu Tyr Gln Leu Asp Lys Glu Gly Arg Ala                 565 570 575 Thr Phe Thr Asn Arg Glu Ser Lys Gln Arg Leu Asp Leu Ala Asn Cys             580 585 590 Gly Leu Ala Asp Gly Thr Asn Phe Ala Gln Ala Pro Ala Leu Asp Thr         595 600 605 Ile Cys Gln Ala Phe Gln Val Arg Tyr Leu Pro     610 615 <210> 134 <211> 1860 <212> DNA <213> Microbulbifer degradans <400> 134 gtggccgcag gccaaataat ttcactggag gttaaagtga aaaagataga agaaataatg 60 aaacacacag cgcgcactat agcgctaggt gcaacaggtg ccgccttgct aacggggtta 120 attgcctgta acggtaccaa tgtgaataca aacggggata cccaacaagc aagcattaaa 180 aaagcgccag aaggcatgtt tgccaacccg ttgttcgcca atggcgcaga cccttggtta 240 gagtattacg atggcaatta ctacctcact accaccacat ggacatcgca acttgttatg 300 cgtaaatcac ccacgttgga tggtttgtct actgcgctgc cggtgaatgt atggtccgat 360 tcagatttaa cccgctgctg taacttttgg gcgtttgaat tccatcgctt aaacggccct 420 aacggctggc gttggtattt aatgtacacc tcgggccagc acggcacttt agatcaccaa 480 cacttaagcg tattagaaag tgtgggtgac gaccctatgg ggccatacac ctacaaaggt 540 gaaatgatgc ccaatacatg gaatatagac ggcagttatt tagagcataa tggccaatta 600 tatttgttgt ggtctgaatg ggtaggtgac gagcagcaaa actttatatc taaaatgacc 660 accccatgga gcattgaagg cccgcgagca ctgttaactc ggccggaagc agagtgggaa 720 aaaagcggtc gcaaagttaa cgaaggccca gagattctaa aaaaagatgg tcgtaccttt 780 ttgatttact cggcgagcta ttgcgatacg ccagattata aactggcaat gaaagagcta 840 acgggcgacg acccaatgaa ctccgagcac tggactaaat acgataagcc cgtgtttgaa 900 agagggaacg gtgtgtttgc cccaggccac aatggtttct tcaaatcgcc cgatggcaca 960 gaagattgga ttgtgtacca cggaaattcg aaagaagagc acggctgcgg tgcaacgcgc 1020 tctgtgcgcg cacagaaatt tacttggaac acagacggca ccccaaattt tggtgagcca 1080 ataccagaag gccaattttt gcccttacct tctggcgaaa acggtccttt agtgactgca 1140 ctgcaaggtg cacgtattca gttgcgcaat ggcgaaagct gtttattagc cgaaggtaaa 1200 gagctgaagc agggcagttg ccaagcagaa gccagtttat gggtaatgga taacacggca 1260 gataatcact accgctttgg caatgtagcc agcaatttat ttttaacggc agacgaaggc 1320 cttagtcaga gtgcctgggt taatactgca agccagcgct gggcacttaa tgcaggcgaa 1380 ggtaactttg ttgcgtttac aaacaagtac accggcgatg cgcttatgca aaataattgg 1440 caaatactac cggttggcaa agtggcaatt agcagcattc aaagtggccg cgtattacag 1500 gcgtgcgata aaaacagcgc gaatgtaaac caaggcggct ggcagggtag ggcttgtcaa 1560 gcatggcaat ttaacccagc aagtgaaggc catgtgcaaa ttaaaacggg caaccaatgt 1620 ttaaccgttg agaataaatc catagtgcct ggcaccaatg ttattgccgg cgagtgtgaa 1680 tcaactagca gccaatggct ttatcaatta gataaagaag gccgcgcaac cttcacaaac 1740 cgcgaaagca aacagcgttt agatttagca aactgcggcc tagccgacgg caccaacttc 1800 gcacaagccc ctgcgttaga tactatttgc caagcattcc aagtgcgtta tttaccgtaa 1860 <210> 135 <211> 534 <212> PRT <213> Microbulbifer degradans <400> 135 Met Asn Pro Ala Ser Thr Leu Ser Val Lys Thr Thr Asn Lys Thr Thr 1 5 10 15 Asn His Leu Lys Lys Val Ala Leu Thr Val Ala Ala Ile Val Ala Pro             20 25 30 Leu Thr Ser Trp Ala Asp Val Lys Val Ser Leu Asn Pro Gln Asn Thr         35 40 45 Gly Glu Thr Ile Ser Lys Tyr Ile Tyr Gly Gln Phe Ala Glu His Leu     50 55 60 Gly Ser Gly Ile Tyr Gly Gly Ile Trp Val Gly Glu Asp Ser Pro Ile 65 70 75 80 Pro Asn Lys Asn Gly Phe Arg Asn Asp Val Ile Lys Ala Leu Gln Glu                 85 90 95 Leu Gln Val Pro Val Ile Arg Trp Pro Gly Gly Cys Phe Ala Asp Glu             100 105 110 Tyr Arg Trp Arg Asp Gly Ile Gly Pro Arg Glu Gln Arg Pro Ile Arg         115 120 125 Val Asn Thr His Trp Gly Gly Val Glu Glu Pro Asn Thr Phe Gly Thr     130 135 140 His Glu Phe Phe Glu Leu Val Glu Leu Leu Asn Thr Glu Ala Tyr Val 145 150 155 160 Ala Gly Asn Leu Gly Thr Gly Ser Pro Gln Glu Met Ala Glu Trp Leu                 165 170 175 Glu Tyr Ile Val Ser Asn Ser Asn Ser Thr Val Val Ala Glu Arg Lys             180 185 190 Lys Asn Gly Arg Glu Glu Pro Trp Glu Val Ala Phe Trp Gly Val Gly         195 200 205 Asn Glu Ser Trp Gly Cys Gly Gly Asn Leu Thr Pro Glu Tyr Tyr Thr     210 215 220 Asn Leu Tyr Arg His Phe Ser Thr Phe Val Lys Ala Thr Gly Ala Lys 225 230 235 240 Arg Pro Lys Leu Val Ala Ser Gly Ser Tyr Asp Asp Asp Glu Thr Trp                 245 250 255 Thr Thr Pro Leu Ser Lys Leu Lys Asn Asn Ile Asp Gly Ile Ser His             260 265 270 His Tyr Tyr Thr Leu Pro Thr Ser Asp Trp Ser Ile Lys Gly Ala Ala         275 280 285 Thr Gly Phe Asp Glu Lys Glu Trp Ile Leu Thr Leu Glu Arg Thr Leu     290 295 300 Lys Ile Asp Ser Tyr Leu Ala Thr Gln Thr Gly Ile Leu Lys Lys Asn 305 310 315 320 Asn Pro Glu Gly Asn Ile Gly Leu Tyr Leu Asp Glu Trp Gly Thr Trp                 325 330 335 Tyr Asp Ala Glu Pro Gly Thr Asn Pro Gly Phe Leu Tyr Gln Gln Asn             340 345 350 Thr Val Arg Asp Ala Ile Val Ala Ala Val Asn Leu Asn Ile Phe His         355 360 365 Asn Tyr Ala Asp Arg Leu His Met Ala Asn Ile Ala Gln Met Val Asn     370 375 380 Val Leu Gln Ala Met Ile Leu Thr Asp Asn Glu Lys Met Leu Leu Thr 385 390 395 400 Pro Thr Tyr His Val Phe Lys Met Tyr Ile Pro Phe Gln Asp Ala Thr                 405 410 415 His Ile Pro Leu Asp Ile Lys Gly Gln Arg Asp Tyr Ser Ala His Lys             420 425 430 Thr Thr Val Pro Gly Phe Ser Ala Ser Ala Ala Lys Thr Pro Asn Gly         435 440 445 Asn Ile Val Val Ser Leu Val Asn Leu Asn Pro Asn Glu Ala Glu Glu     450 455 460 Val Ser Ile Ala Leu Gln Gly Ile Lys Val Lys Thr Ile Thr Gly Glu 465 470 475 480 Leu Leu Thr Ser Gln Lys Met Asp Ala His Asn Thr Phe Asp Lys Pro                 485 490 495 Asn Asn Val Gln Pro Arg Ala Leu Asn Gln Ser Asp Tyr Ser Ile Ser             500 505 510 Lys Asn Gly Lys Thr Leu Thr Val Lys Leu Pro Ala Lys Ala Val Val         515 520 525 Val Leu Gln Leu Asn Lys     530 <210> 136 <211> 1605 <212> DNA <213> Microbulbifer degradans <400> 136 atgaacccag cttcaaccct aagtgtgaaa acaactaata aaacaacaaa ccacctcaaa 60 aaagttgccc ttaccgtagc cgctatagtt gccccgctaa caagctgggc cgatgtgaaa 120 gttagcctaa acccacaaaa cacaggcgaa accataagta aatatatcta cggccaattc 180 gcagagcacc ttggcagcgg catatacggc ggcatatggg tgggcgaaga ctccccaata 240 cccaacaaaa acggctttcg taacgatgta atcaaagccc tgcaagagct acaagtacct 300 gttataagat ggcccggtgg ctgctttgcc gacgaatatc gctggcgtga tggcattggc 360 ccacgtgagc aacgccctat ccgcgtaaat acccactggg gcggtgtgga agaacccaat 420 acctttggta ctcacgaatt cttcgaatta gttgagctac ttaataccga agcctatgtg 480 gcaggtaacc taggcactgg ctcaccacaa gaaatggccg aatggctgga atatattgtt 540 tccaactcca actctactgt agtggcagag cgcaaaaaaa atggccgcga agagccttgg 600 gaagtggcct tttggggtgt gggtaatgaa tcttggggtt gcggtggcaa cttaacgccc 660 gagtactaca ccaatttata tcgtcatttt tccacttttg ttaaagccac tggcgctaag 720 cgcccgaagt tagttgcaag cggctcgtat gatgatgacg aaacttggac aacgccacta 780 agtaagctca aaaataatat agatggtata agccaccact actacacctt acccaccagc 840 gactggagca taaaaggtgc ggctacaggc tttgatgaaa aagaatggat tcttaccctt 900 gagcgcacat tgaaaataga cagctacctt gcaactcaaa cgggtattct taaaaagaat 960 aaccccgaag gcaatatagg gttgtattta gatgaatggg gtacgtggta cgatgcagag 1020 cccggtacaa accccggctt tttataccaa caaaataccg tgcgcgacgc catagtagca 1080 gcagtaaact taaatatatt ccacaactat gccgaccgct tacacatggc gaacatcgcc 1140 caaatggtaa acgtactaca ggcaatgata ctgaccgaca acgaaaaaat gttgctcaca 1200 cccacgtatc acgtttttaa gatgtacata ccatttcaag atgctaccca tataccgtta 1260 gatataaaag gccagcgcga ctacagcgca cataaaacta ccgtaccagg gttctcggcc 1320 tcggcagcta aaaccccaaa tggcaatatt gtagtttcac ttgttaacct taacccaaac 1380 gaagcggaag aagtgagcat tgcactacaa ggcatcaagg taaaaaccat tactggtgaa 1440 ttacttacca gccaaaaaat ggatgcgcat aacaccttcg acaagccaaa caatgtgcag 1500 ccacgcgcac ttaaccaaag cgactacagc attagcaaaa acggcaaaac ccttaccgtt 1560 aagctacccg ctaaagccgt agttgtttta cagcttaata aataa 1605 <210> 137 <211> 397 <212> PRT <213> Microbulbifer degradans <400> 137 Met Leu Leu Ala Gln Leu Pro Val Lys Lys Tyr Phe Val Leu Leu Ala 1 5 10 15 Ile Phe Ser Phe Met Leu Gly Cys Asn Ser Ala Gly Val Gln Gln Ser             20 25 30 Ala Lys Ser Ile Gln Val Ala Gly Thr His Ser Lys Pro Ala Arg Phe         35 40 45 Phe Ala Gly Ala Asp Leu Ser Tyr Val Asn Glu Met Glu Asp Cys Gly     50 55 60 Ala Thr Tyr Arg Val Asn Gly Val Thr Thr Asp Pro Tyr Gln Ala Phe 65 70 75 80 Ala Asp Ala Gly Ala Asn Leu Val Arg Val Arg Leu Trp His Asn Pro                 85 90 95 Thr Trp Thr Glu Tyr Ser Asp Phe Ala Asp Val Lys Lys Thr Ile Arg             100 105 110 Lys Ala Lys Gln Asn Asn Gln Thr Val Leu Leu Asp Phe His Tyr Ser         115 120 125 Asp Thr Trp Ala Asp Pro Glu Lys Gln Phe Val Pro Ala Ala Trp Glu     130 135 140 His Met Val Asp Asp Thr Pro Ala Leu Ala Gln Ala Leu Ala Gln Tyr 145 150 155 160 Thr Thr Asp Val Leu Glu Lys Leu Gln Ala Glu Asn Leu Leu Pro Asp                 165 170 175 Met Val Gln Val Gly Asn Glu Thr Asn Ala Glu Val Leu Gln Leu Glu             180 185 190 Ala His Met Lys His Gly Glu Ile Asp Trp Gln Arg Asn Ala Ala Leu         195 200 205 Leu Asn Ser Gly Leu Ala Ala Val Ala Glu Phe Asn Gln Asn Asn Asn     210 215 220 Thr Tyr Ile Glu Arg Val Leu His Ile Ala Gln Pro Glu Asn Ala Leu 225 230 235 240 Trp Trp Phe Asp Asp Ala Ala Gln Ala Gly Ile Thr Asp Phe Glu Ile                 245 250 255 Ile Gly Leu Ser Tyr Tyr Ala Lys Trp Ser Thr Tyr Lys Leu Asp Ser             260 265 270 Ile Gly Glu Ala Ile Arg Ala Leu Arg Thr Ala Phe Asn Lys Asp Val         275 280 285 Leu Val Val Glu Thr Ser Tyr Pro Trp Thr Met Gln Asn Phe Asp Gln     290 295 300 Ala Asn Asn Val Leu Asp Ala Thr Ser Leu Gln Gln Gly Tyr Pro Ala 305 310 315 320 Thr Ala Glu Gly Gln Lys Lys Tyr Met Met Asp Leu Ala Lys Gln Ile                 325 330 335 Met Tyr Ala Gly Gly Ile Gly Ile Ala Tyr Trp Glu Pro Ala Trp Val             340 345 350 Ser Thr Pro Cys Lys Thr Leu Trp Gly Thr Gly Ser His Trp Glu Asn         355 360 365 Ala Val Phe Phe Asp Ser Gly Asn Asn Asn Glu Ala Leu Pro Ala Leu     370 375 380 Ser Phe Tyr Thr Asp Ile Met Ala Leu Phe Lys Gln Asp 385 390 395 <210> 138 <211> 1194 <212> DNA <213> Microbulbifer degradans <400> 138 atgttattag ctcaattgcc agttaaaaaa tattttgtct tattagctat tttctcgttt 60 atgctggggt gtaatagtgc tggcgtacaa caaagtgcta aatcaattca ggttgctggc 120 acgcacagta aacccgctcg tttttttgct ggtgccgacc tttcttacgt aaacgaaatg 180 gaagattgcg gagcaacata ccgcgtaaac ggtgtaacta ccgaccctta ccaagccttt 240 gccgatgccg gcgcaaattt agtgcgcgtg cgcttatggc acaaccctac ttggacagaa 300 tattccgact ttgccgacgt taaaaaaact atccgcaaag ccaaacaaaa taatcaaacg 360 gtattgttag attttcatta ttcagatacc tgggccgacc cagaaaaaca atttgttcca 420 gccgcttggg aacatatggt ggatgacacc ccagcactag cgcaagcctt agcgcaatac 480 acaaccgatg tattagaaaa gctgcaagca gaaaacctat tgccagatat ggtgcaagta 540 ggtaacgaaa caaacgcaga agtcttacag ctagaagcgc acatgaaaca cggcgaaata 600 gattggcagc gcaatgcagc gctactaaac agtgggttag cagccgttgc tgaatttaac 660 caaaacaaca acacctatat tgaacgcgta ttacatatcg cccagccaga aaatgctttg 720 tggtggtttg acgatgccgc gcaggctggc ataaccgatt ttgaaattat aggtcttagc 780 tactatgcca aatggtcaac gtataaatta gattccatcg gcgaagctat acgcgccttg 840 cgaaccgcat tcaataaaga tgtgttggtg gtagaaacct catacccctg gactatgcaa 900 aatttcgatc aagccaataa cgtgctcgat gctaccagct tgcagcaggg ctaccctgca 960 acggccgaag gccaaaaaaa atacatgatg gatttagcta aacaaattat gtacgccggt 1020 ggaattggta ttgcctactg ggaaccagct tgggtaagca ccccttgcaa aactctatgg 1080 ggtacaggtt ctcactggga aaatgccgtg ttttttgact ctggcaacaa caacgaagcg 1140 ctacccgcgc ttagtttcta cacagacata atggctcttt ttaagcaaga ttaa 1194 <139> <211> 420 <212> PRT <213> Microbulbifer degradans <400> 139 Met Leu Gln Ile Leu Lys Asp His Gly Met Asp Ser Ile Arg Leu Arg 1 5 10 15 Val Trp Val Asn Pro Ala Gly Gly Trp Tyr Ser Ser Ile Asn Asp Val             20 25 30 Ile Glu Lys Ala Gln Arg Ala Lys Ala Ala Gly Met Arg Ile Met Ile         35 40 45 Asp Phe His Tyr Ser Asp Ser Trp Ala Asp Pro Gly Lys Gln Tyr Lys     50 55 60 Pro Ala Ala Trp Thr Asn Tyr Thr Leu Asp Gly Leu Met Ser Ala Val 65 70 75 80 Trp Trp His Thr Tyr Asp Ser Leu Val Ala Leu Lys Asn Ala Gly Ile                 85 90 95 Thr Pro Glu Trp Val Gln Val Gly Asn Glu Thr Asn Asn Gly Met Leu             100 105 110 Trp Glu Glu Gly Arg Ala Ser Ala Asn Met Gln Asn Tyr Ala Trp Leu         115 120 125 Val Asn Ser Gly Tyr Asp Ala Val Lys Glu Val Phe Pro Asn Thr Lys     130 135 140 Ala Val Val His Leu Ala Asn Cys His Asp Asn Ala Asn Phe Arg Trp 145 150 155 160 Ile Phe Asp Gly Leu Gln Ala Asn Gly Gly Lys Trp Asp Val Ile Gly                 165 170 175 Ala Ser Ile Tyr Pro Thr Asn Ala Ser Gly Tyr Ser Trp Ser Gln Ala             180 185 190 Asn Ser Leu Cys Glu Ala Asn Leu Asn Asp Met Gln Ser Arg Tyr Gly         195 200 205 Ser Glu Val Leu Ile Ala Glu Val Gly Ala Pro Trp Asp His Pro Glu     210 215 220 Ala Lys Ala Ile Val Ser Asp Val Ile Ala Lys Ala Gln Asn Ala Gly 225 230 235 240 Ala Thr Gly Val Phe Tyr Trp Glu Pro Gln Ala Ser Asn Trp Gln Gly                 245 250 255 Tyr Thr Leu Gly Ala Trp Asn Pro Asn Thr Met Arg Pro Thr Glu Ala             260 265 270 Leu Asp Ala Phe Ile Asp Gly Ser Ser Asn Val Thr Thr Ala Arg Leu         275 280 285 Gln Ser Arg Asn Ser Asn Arg Cys Ile Asp Val Asn Gly Arg Ser Thr     290 295 300 Ala Asp Gly Ala Asp Ile Ile Gln Trp Ser Cys His Ser Asn Ala Asn 305 310 315 320 Gln Gln Trp Thr Phe Glu Asp Met Gly Asn Asn Tyr Val Arg Leu Arg                 325 330 335 Val Gly His Ser Asn Lys Cys Leu Asp Val Leu Gly Ala Gly Thr Ala             340 345 350 Asp Gly Asp Asn Val Val Gln Trp Ala Cys His Asn Asn Ala Asn Gln         355 360 365 Gln Trp Leu Lys Glu Asp Met Gly Asp Gly Tyr Phe Arg Leu Lys Ser     370 375 380 Arg Ala Ser Gly Lys Cys Val Asp Val Asn Ala Gly Gly Ala Asn Asn 385 390 395 400 Gly Asp Ser Ile Ile Gln Trp Ser Cys His Thr Gly Trp Asn Gln Gln                 405 410 415 Trp Met Val Tyr             420 <210> 140 <211> 1263 <212> DNA <213> Microbulbifer degradans <400> 140 gtgctgcaaa ttctaaaaga tcacggtatg gattccatcc gtctgcgcgt gtgggtaaac 60 cccgccggtg gatggtatag cagcattaat gacgtaatag aaaaagctca gcgcgccaaa 120 gctgcgggca tgcgtattat gatcgatttt cactacagcg actcttgggc tgacccaggc 180 aagcaataca agcccgccgc gtggaccaac tataccttag acggtttaat gtctgcggtg 240 tggtggcaca cctacgattc cctcgtggcc ctaaagaatg cgggtattac ccctgaatgg 300 gtgcaagtgg gcaacgaaac aaacaacggt atgttatggg aagaggggcg cgcatccgcc 360 aatatgcaaa actatgcgtg gttggtgaat agtggctacg atgccgttaa agaagtgttc 420 cctaatacca aggcagtggt gcacttggca aactgccacg acaacgcaaa cttccgctgg 480 atatttgacg gcttacaagc caatggtggt aagtgggatg taataggtgc ctctatttac 540 cctaccaacg caagcggtta tagctggagc caagccaaca gtttgtgcga ggcaaactta 600 aacgatatgc aatcgcgcta tgggtccgag gtgctaattg ccgaggttgg tgcgccgtgg 660 gatcacccag aagcgaaagc aatcgtgagc gatgtaattg ctaaggcgca aaacgccggt 720 gcaacagggg tattttattg ggagccgcag gcatcaaact ggcagggcta cacgctaggt 780 gcatggaacc caaacaccat gcgccccacc gaagcattag acgcgtttat tgacggcagc 840 tcgaatgtga caaccgcgcg tttgcaatcg cgcaatagca accgctgtat agatgttaat 900 ggccgcagta cagcagatgg tgccgatatc attcagtgga gttgccacag caacgccaac 960 cagcaatgga cttttgaaga tatgggcaat aactacgtgc gattgcgcgt gggccacagt 1020 aataagtgct tagatgtact gggtgcaggc actgccgatg gcgataacgt agtgcagtgg 1080 gcatgccaca ataacgccaa tcagcaatgg ctaaaagaag acatgggcga tggctacttc 1140 cgcttaaaat ctcgcgccag cggtaaatgc gtagatgtaa acgcaggcgg tgctaacaac 1200 ggtgattcta ttattcaatg gagttgccac actggttgga accagcaatg gatggtttat 1260 tag 1263 <210> 141 <211> 589 <212> PRT <213> Microbulbifer degradans <400> 141 Asn Asp Leu Ser Tyr Val Asn Glu Met Glu Asp Cys Gly Ala Val Tyr 1 5 10 15 Lys Asp Ala Gly Ser Val Val Asp Pro Tyr Glu Val Ile Ala Asn His             20 25 30 Gly Gly Asn Leu Val Arg Val Arg His Trp Asn Asp Pro Tyr Trp Gln         35 40 45 Ala Leu Ile Thr Gln Pro Glu Ser Val Ala Ala Asn Trp Lys Ala Asn     50 55 60 Tyr Ser Gly Leu Glu Asp Val Thr Glu Thr Ile Arg Arg Ser Lys Ala 65 70 75 80 Ala Gly Met Glu Val Leu Leu Asp Phe His Phe Ser Asp Ile Trp Ala                 85 90 95 Asp Pro Gly Arg Gln Thr Thr Pro Arg Ala Trp Glu Asn Asp Phe Gly             100 105 110 Asp Glu Asp Ala Met Ala Ala His Ile Tyr Asp Tyr Val Thr Ser Val         115 120 125 Leu Thr Gly Leu Asn Asp Glu Gly Leu Met Pro Glu Leu Ile Gln Ile     130 135 140 Gly Asn Glu Ser Asn Ser Gly Met Met Thr Thr Gln Asn Leu Ile Ile 145 150 155 160 Glu Met Asn Asp Ala Gly Thr Gly Leu Asn Val Ser Lys Gly Gly Gln                 165 170 175 Thr Asn Tyr Ser Asp Gln Tyr Val Ala Arg Met Tyr Asn Ser Ala Ile             180 185 190 Ser Ala Val Arg Asp Ile Ser Glu Gly Met Thr Asn Ala Pro Arg Ile         195 200 205 Ala Ile His Val Ala Gly Ala Asp Lys Ala Val Ala Phe Phe Asp Lys     210 215 220 Leu Lys Ser Ile Gly Val Thr Asp Ile Asp Ile Ala Gly Phe Ser Phe 225 230 235 240 Tyr Tyr Gly Trp Glu Gln Ala Pro Ile Glu Asp Val Ala Ser Met Ile                 245 250 255 Ala Thr Leu Lys Glu Arg His Pro Asn Leu Asp Pro Leu Met Leu Glu             260 265 270 Thr Gly Tyr Leu Trp Asp Glu Glu Asn Ile Asp Ser Leu Gly Asn Ile         275 280 285 Ile Gly Ile Ala Asp Pro Ala Tyr Leu Pro Val Ser Lys Gln Asn Gln     290 295 300 Leu Lys Tyr Leu Thr Asp Leu Ser Gln Ala Val Ala Asp Ala Gly Gly 305 310 315 320 Ile Gly Val Val Phe Trp Glu Pro Ser Trp Val Ser Thr Glu Cys Arg                 325 330 335 Thr Pro Trp Gly Gln Gly Ser Ser His Glu His Val Ala Tyr Phe Asp             340 345 350 His Arg Asp Gly Leu Asn Phe His Ile Gly Gly Gln Trp Met Glu Val         355 360 365 Thr Lys Leu Ser Glu Thr Pro Glu Ala Gly Leu Ala Thr Thr Phe Arg     370 375 380 Val Asp Met Thr Gly Gln Asp Thr Ser Ala Gly Val Phe Ile Arg Gly 385 390 395 400 Ala Phe Thr Glu Asp Thr Leu Gln Pro Met Leu Tyr Glu Gly Glu Asn                 405 410 415 Ile Tyr Ser Tyr Thr Thr His Ile Gln Ala Ala Gln Ser Gly Ser Tyr             420 425 430 His Tyr Ala Ile Gly Leu Lys Asn Gly Thr Arg Glu Thr Val Pro Ser         435 440 445 Glu Cys Ala Asn Pro Glu Asp Thr Leu Asn Arg Leu Tyr Thr Val Gly     450 455 460 Glu Asn Gly Glu Gln Leu Val Thr Ala Val Trp Ala Ser Cys Asp Val 465 470 475 480 Phe Asp Pro Gln Ala Ala Gly Pro Thr Thr Leu Thr Leu Asn Val Asp                 485 490 495 Met Thr Gly Val Asp Val Ser Gly Val Tyr Val Ala Gly Asp Leu             500 505 510 Asn Ala Trp Thr Ile Thr Glu Leu Thr Gln Val Gly Ala Ser Ala Ile         515 520 525 Tyr Thr Ile Ser Tyr Asp Leu Ala Val Gly Ala Glu Gly Gly Tyr Tyr     530 535 540 Phe Leu Asn Gly Ser Asp Trp Gly Asp Arg Glu Thr Ile Pro Glu Glu 545 550 555 560 Cys Val Gly Tyr Tyr Asp Ala Asp Arg Gly Phe Leu Val Glu Glu Gln                 565 570 575 Ser Pro Gln Val Leu Asp Leu Val Trp Ser Ser Cys Gln             580 585 <210> 142 <211> 1770 <212> DNA <213> Microbulbifer degradans <400> 142 aacgaccttt cctacgtaaa cgaaatggaa gactgcggcg cagtgtataa agatgcaggc 60 agtgtagtag acccctacga agtgatcgcc aatcacggcg gcaaccttgt gcgcgtgcgt 120 cactggaacg acccctactg gcaggcgctt attacccagc cagaatctgt agcggcaaat 180 tggaaagcta attacagtgg gcttgaagat gtaacagaaa caattcgacg atcaaaagcc 240 gccggtatgg aagtgctgct cgactttcat ttctcagata tttgggcaga ccccggcagg 300 caaacaacgc cgcgcgcatg ggaaaatgac tttggcgatg aagacgccat ggctgcgcat 360 atatacgatt acgtaacatc agtactcaca gggttaaacg acgaaggttt aatgcccgag 420 cttattcaaa ttggtaacga atctaactcc ggcatgatga cgactcaaaa cctcattata 480 gaaatgaatg atgcgggtac ggggttaaat gtgagtaaag gcgggcaaac aaattactca 540 gatcaatatg ttgcgcgtat gtataactct gcgatttctg ctgtgcgcga tattagtgaa 600 gggatgacca acgctccgcg tattgctatc cacgtggcag gtgcagataa agccgtcgca 660 ttttttgata agttaaaaag catcggagta acggatattg atatcgcggg cttctcgttt 720 tattacggtt gggagcaagc accaatagaa gacgttgcaa gcatgattgc aaccttaaag 780 gaacgtcacc ctaatttaga cccgttaatg cttgaaacag gctacctgtg ggatgaagaa 840 aacatcgata gcttaggcaa tattattggc attgccgacc ccgcatattt acctgtgagc 900 aaacagaacc aacttaaata tcttaccgat ttatcgcaag cggttgctga tgctggtggt 960 attggagtgg tgttttggga gccatcttgg gtatcaaccg aatgtcgcac accttggggg 1020 cagggctcat ctcacgagca tgttgcttac ttcgatcacc gcgacggctt aaactttcat 1080 attggtggcc aatggatgga ggttaccaag ttaagcgaaa ccccagaagc ggggctagct 1140 actaccttta gagtggatat gactggccaa gacaccagcg caggggtatt tattcgcggg 1200 gcgtttaccg aagacacatt gcagcccatg ctatatgaag gcgaaaacat ttatagttac 1260 accacgcata tccaagcagc gcaaagcgga agctaccact atgcgattgg cttaaaaaat 1320 ggtacgcgcg aaacggttcc tagcgaatgt gcaaacccag aagatacgtt aaatcgttta 1380 tatacggtgg gcgaaaatgg ggagcagtta gttaccgcag tttgggcaag ctgtgatgtt 1440 ttcgatccgc aagcggctgg gccaacaacc ttaacgctta atgtagatat gactggtgta 1500 gatgtaagtg gtggtgtgta tgttgcaggc gacttaaatg cttggacaat caccgagctt 1560 acacaagttg gcgctagcgc aatttatacc attagttacg atttagccgt aggtgcagaa 1620 ggtggctact acttcttgaa tggcagcgat tggggcgata gggaaacaat accagaagaa 1680 tgtgtgggct actatgatgc agaccgcggc tttttggtgg aagagcaaag cccacaggta 1740 ttggatttag tgtggagtag ttgtcaataa 1770 <210> 143 <211> 339 <212> PRT <213> Microbulbifer degradans <400> 143 Met Glu Arg Thr Gln Thr Ala Met Ser Asp Thr Pro Val Asn Pro Asn 1 5 10 15 Ala Asn Thr Thr Thr Lys Ala Val Tyr Thr Tyr Leu Lys Gln Gln Trp             20 25 30 Gly Ser Lys Met Leu Thr Gly Gln Met Asp Leu Thr Trp Lys Asp Ser         35 40 45 Ile Asp Glu Tyr Gln Arg Val Ile Asn Asp Thr Gly Lys Ala Pro Ala     50 55 60 Ile Met Gly Tyr Asp Tyr Met Asn Tyr Gly Ile Glu Ser Ser Phe Ile 65 70 75 80 Ser Gly Leu Glu Gln Thr Glu Glu Ala Ile Val His Trp Gln Arg Gly                 85 90 95 Gly Leu Val Thr Phe Ala Trp His Trp Arg Asp Pro Asn Val Ser Gly             100 105 110 Ser Asn Ile Gly Glu Phe Tyr Thr Ala Asp Thr Ser Phe Gln Ile Pro         115 120 125 Ile Ala Asn Gly Gln Leu Asp Glu Ser Ser Gln Ser Phe Ile Asn Met     130 135 140 Gln Ala Asp Ile Asp Met Ile Ala Ala Glu Leu Gln Lys Leu Glu Asp 145 150 155 160 Ala Gly Ala Val Val Leu Trp Arg Pro Leu His Glu Ala Ser Gly Gly                 165 170 175 Trp Phe Trp Trp Gly Arg Thr Arg Thr Asp Ser Val Ser Ala Ala Tyr             180 185 190 Ala Gln Val Leu Leu Trp Arg His Met Tyr Thr Arg Leu Thr Asp His         195 200 205 His Gly Leu Asp Asn Leu Leu Trp Val Trp Asn Gly Gln Asn Ser Ala     210 215 220 Trp Tyr Pro Gly Asp Glu Tyr Ala Asp Ile Val Ser His Asp Ile Tyr 225 230 235 240 Asp Gly Ala Lys Asn Tyr Glu Ser Gln Leu Ala Val Tyr Asn Asp Thr                 245 250 255 Lys Asn Thr Pro Met Gln Thr Lys Met Val Ala Leu Ser Glu Asn Ser             260 265 270 Asn Ile Pro Asp Pro Asp Ala Met Gln Ala Asp Gly Ala Trp Trp Leu         275 280 285 Trp Phe Met Val Trp Asn Asp Ser Asp Thr Ala Glu Gly Val Thr His     290 295 300 Glu Asn Asn Phe Trp Thr Gly Glu Tyr Tyr Asn Ser Asn Ala His Lys 305 310 315 320 Gln His Val Tyr Asn His Glu Leu Val Ile Thr Leu Asp Glu Leu Pro                 325 330 335 Ser Phe Asp              <210> 144 <211> 1020 <212> DNA <213> Microbulbifer degradans <400> 144 atggagcgca ctcaaacggc catgagcgat acgccggtaa accctaacgc caataccacc 60 accaaggctg tttacactta ccttaagcag caatggggca gcaaaatgct aaccgggcag 120 atggatttga cctggaagga cagcattgat gagtaccagc gcgtaattaa cgataccggc 180 aaagcccccg caattatggg ctacgactat atgaattacg gtattgagag cagttttatt 240 agtggccttg agcaaacaga agaagctatt gtccactggc agcgcggcgg cttagtgact 300 tttgcttggc actggcgaga cccgaacgta agcggtagta acattggcga gttttatacg 360 gctgatacga gctttcaaat cccaattgcc aatggtcaac tggatgaaag cagtcagagc 420 tttataaata tgcaggccga tatcgatatg atagcggcag agctgcaaaa gctagaagat 480 gccggcgctg tggtgctgtg gcgccccttg cacgaagctt ctggcggttg gttctggtgg 540 ggccgtacgc gtaccgattc ggtatctgcc gcttatgcac aagtgctgtt gtggcgccac 600 atgtacaccc gccttactga tcatcacggt ttagataatt tactttgggt gtggaacggg 660 caaaacagcg cttggtaccc aggcgatgaa tatgccgata ttgtaagtca cgacatttac 720 gacggcgcga aaaattacga gtcgcaatta gccgtatata acgatacgaa aaacaccccc 780 atgcaaacca aaatggtggc gctaagcgag aacagtaata ttcccgaccc agatgccatg 840 caggccgatg gcgcttggtg gttgtggttt atggtgtgga acgactcgga taccgcggaa 900 ggcgtaaccc acgaaaacaa cttttggaca ggtgaatatt acaactctaa cgcccataag 960 cagcatgttt acaatcatga gctggttatt acgctggatg agttacctag ctttgactag 1020 <210> 145 <211> 561 <212> PRT <213> Microbulbifer degradans <400> 145 Met Phe Asn Lys Ile Ser Thr Pro Ala Leu Ser Gly Trp Ser Lys Ala 1 5 10 15 Ala Arg Tyr Leu Cys His Thr Ala Thr Gly Ala Leu Met Leu Ala Ala             20 25 30 Ser Thr Val Asn Ala Gly Phe Ser Val Ser Gly Thr Gln Leu Leu Asp         35 40 45 Asp Asn Gly Gln Ala Phe Ile Met Arg Gly Val Asn His Pro His Ala     50 55 60 Trp Tyr Ala Asn Gln Thr Ser Ser Phe Ala Asp Ile Ala Ser Val Gly 65 70 75 80 Ala Asn Thr Val Arg Val Val Leu Ser Asp Gly Gln Gln Trp Thr Arg                 85 90 95 Asn Ser Ala Ser Asp Val Ala Asn Val Ile Ser Leu Cys Lys Ala Asn             100 105 110 Lys Leu Val Cys Val Leu Glu Val His Asp Val Thr Gly Ser Gly Glu         115 120 125 Ala Ser Ala Ala Gly Thr Leu Ala Asn Ala Ala Gln Tyr Trp Val Asp     130 135 140 Ile Ala Asn Val Leu Lys Gly Gln Glu Asp Tyr Val Ile Ile Asn Ile 145 150 155 160 Ala Asn Glu Pro Phe Gly Asn Asn Val Pro Ala Ser Asn Trp Ile Asn                 165 170 175 Gln His Lys Ala Ala Ile Gln Thr Leu Arg Ala Ala Gly Leu Thr His             180 185 190 Thr Leu Met Ile Asp Ala Ala Asn Trp Gly Gln Asp Trp Gln Gln Val         195 200 205 Met Leu Asn Asn Ala Ser Glu Val Ala Gln Ala Asp Ser Leu Ser Asn     210 215 220 Thr Met Phe Ser Val His Met Tyr Gln Val Tyr Asn Asn Leu Ser Thr 225 230 235 240 Val Glu Asn Tyr Val Ser Thr Phe Leu Ser Ser His Asn Leu Pro Leu                 245 250 255 Ile Val Gly Glu Phe Gly Ala Asp His Gln Gly Glu Glu Val Asp Glu             260 265 270 Asp Ala Ile Leu Ser Val Ala Glu Gln Tyr Gly Ile Gly Tyr Leu Gly         275 280 285 Trp Ser Trp Ser Gly Asn Gly Ser Cys Cys Gly Thr Leu Asp Ile Thr     290 295 300 Asn Asn Phe Asn Val Asn Ser Leu Thr Ser Trp Gly Asn Arg Leu Ile 305 310 315 320 Asn Gly Thr Asn Gly Ile Lys Ala Thr Ser Val Ile Ala Ser Val Tyr                 325 330 335 Gly Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Thr Ser             340 345 350 Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Gly         355 360 365 Ser Ser Gly Gly Ala Gln Gln Cys Asn Trp Tyr Gly Ser Val Tyr Pro     370 375 380 Leu Cys Asn Asn Gln Ala Ser Gly Trp Gly Trp Glu Asn Gln Gln Ser 385 390 395 400 Cys Ile Gly Arg Thr Thr Cys Glu Ser Gln Ser Gly Asn Gly Gly Val                 405 410 415 Ile Gly Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser             420 425 430 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser         435 440 445 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly Gly Ser     450 455 460 Gly Ala Thr Cys Glu His Ile Ile Thr Asn Ser Trp Asn Ser Gly Phe 465 470 475 480 Gln Gly Ala Val Arg Ile Thr Asn Asn Gly Ser Ser Ala Ile Asn Gly                 485 490 495 Trp Gln Val Ser Trp Ser Tyr Ser Asp Gly Thr Thr Ile Gly Ser Val             500 505 510 Trp Asn Ala Asn Gln Ser Gly Ser Asn Pro Tyr Thr Ala Ser Asn Leu         515 520 525 Gly Trp Asn Ala Thr Val Asn Pro Gly Gln Ser Val Glu Phe Gly Phe     530 535 540 Thr Ala Asn Gly Gly Gly Ala Ala Ser Ala Val Thr Gly Ser Val Cys 545 550 555 560 Asn      <210> 146 <211> 61 <212> PRT <213> Microbulbifer degradans <400> 146 Met Cys Ser Gln Val Ala Pro Leu Pro Pro Asp Glu Leu Leu Glu Glu 1 5 10 15 Glu Glu Leu Glu Asp Asp Glu Leu Leu Leu Asp Glu Leu Glu Leu Leu             20 25 30 Leu Asp Glu Leu Leu Glu Glu Leu Leu Asp Glu Leu Leu Glu Glu Leu         35 40 45 Leu Asp Glu Asp Pro Pro Ile Thr Pro Pro Leu Pro Asp     50 55 60 <210> 147 <211> 1686 <212> DNA <213> Microbulbifer degradans <400> 147 atgtttaaca agatatctac acccgcgtta agcggttgga gcaaggctgc gcgctattta 60 tgccatacgg ctactggcgc attaatgttg gctgcaagta cggtaaatgc ggggtttagc 120 gtttcgggta cgcagctgtt agatgataac ggccaagcgt ttattatgcg cggtgttaat 180 catccgcatg cgtggtacgc caatcaaacc agctcgtttg ctgatatagc ttcagtaggt 240 gctaatacgg ttcgagtggt actaagcgat ggccagcaat ggacgcgcaa cagcgcatct 300 gatgtggcga atgttatctc gctatgtaaa gcaaacaagt tagtgtgtgt tcttgaggta 360 cacgatgtaa ccggttcagg cgaggccagt gcggcgggta ccttggcaaa tgcggcccag 420 tattgggtag atattgccaa cgtgcttaag gggcaagaag attacgtaat aatcaatatt 480 gccaacgaac cgttcggcaa caatgtgcct gccagtaatt ggattaacca gcacaaagcg 540 gctattcaaa cgctgcgagc ggccggttta acccacacgc taatgataga tgcggcaaac 600 tgggggcaag attggcaaca agtaatgcta aacaatgctt cagaggtggc acaggccgat 660 agcctaagta acaccatgtt tagcgtgcat atgtatcagg tttacaacaa cttaagcacc 720 gtagaaaact atgtgtctac gtttttaagt agccataact tgccgttaat agtgggggag 780 tttggtgcag atcaccaagg tgaagaggtt gatgaagatg caattttatc tgtggcagag 840 cagtatggca ttggctactt aggctggagt tggtcgggca atggcagttg ttgcggcacc 900 ttagatataa ccaacaactt taacgttaac agtttaacca gttggggtaa ccgcctaata 960 aacggtacca atggtattaa agcaacctcg gtaattgcat ctgtatacgg tggctcgtcg 1020 agcagttcta gttcatctag tagctcttca actagctcta gcagttccag ttcttctacc 1080 agttcatcca gcagttcgtc cggttctagt ggtggtgcgc aacagtgtaa ttggtacggc 1140 tcggtttacc cattgtgtaa taatcaagcc agtggttggg gctgggaaaa tcagcaaagc 1200 tgtattggcc gtactacctg cgaaagtcag tctggtaatg gcggagttat tggcgggtct 1260 tcgtcgagca gttcttctag tagttcatca agcagttctt ctagcagctc atccagtagc 1320 agttctagct catctagcag cagctcgtcg tcctcaagtt cttcttcttc tagtagttca 1380 tcgggcggca gtggtgcaac ttgcgaacac ataattacaa atagttggaa cagcggtttt 1440 caaggggcgg tgcgcattac caataatggc agcagtgcca ttaacggctg gcaagtaagt 1500 tggagctaca gtgatggcac aaccattggc agtgtatgga atgccaatca aagcggtagc 1560 aacccttaca cagccagcaa tttagggtgg aacgccacgg ttaaccctgg gcagtcggta 1620 gagtttggtt ttactgccaa cggcggcggt gctgcatcag cagtgacggg gtcggtttgt 1680 aactaa 1686 <210> 148 <211> 514 <212> PRT <213> Microbulbifer degradans <400> 148 Met Asn Asn Glu Glu Val Asn Met Arg Ile Phe Ser Ile Ala Leu Ala 1 5 10 15 Leu Cys Ala Val Leu Cys Ala Gly Gln Ser Leu Ala Gly Leu Ser Ile             20 25 30 Gln Gly Thr Arg Leu Val Asp Gly Asn Gly Ser Thr Val Val Leu Arg         35 40 45 Gly Val Asn His Pro His Ala Trp Tyr Ala Gly Glu Thr Ala Ala Ala     50 55 60 Ile Pro Lys Ile Ala Ala Thr Gly Ala Asn Ser Val Arg Val Val Met 65 70 75 80 Ala Met Gly Thr Lys Trp Ser Arg Thr Ser Ala Ala Glu Ile Gln Thr                 85 90 95 Ile Ile Asp Leu Cys Lys Gln Asn Asn Met Ile Ala Val Leu Glu Phe             100 105 110 His Asp Gly Thr Gly Trp Gly Glu Glu Ser Gly Thr Ala His Ile Ser         115 120 125 Asp Ile Ala Asp Tyr Trp Val Ser Ser Asp Val Met Ala Val Val Lys     130 135 140 Gly Glu Glu Asp Tyr Val Ile Ile Asn Ile Ala Asn Glu Pro Phe Gly 145 150 155 160 Asn Gly Val Ser Ala Ser Thr Tyr Thr Asn Asp Thr Ile Ala Ala Ile                 165 170 175 Gln Lys Leu Arg Asn Ala Gly Tyr Thr His Thr Leu Met Val Asp Ala             180 185 190 Ala Asn Trp Gly Gln Asp Trp Gln Asn Leu Met Arg Asp Asn Ala Gln         195 200 205 Thr Ile Phe Asn Gly Asp Ser Leu Asn Asn Thr Met Phe Ser Val His     210 215 220 Met Tyr Gln Val Tyr Asn Thr Ser Ala Lys Val Gln Ser Tyr Met Gln 225 230 235 240 Ala Phe Ser Asp Lys Gly Leu Ala Leu Val Val Gly Glu Phe Ala Ala                 245 250 255 Asp His Phe Ser Glu Asp Val Ala Glu Ala Ala Ile Met Gln Tyr Ala             260 265 270 Glu Gln Phe Gly Phe Gly Tyr Met Gly Trp Ser Trp Thr Gly Asn Ser         275 280 285 Ser Asp Leu Ala Ser Leu Asp Ile Val Lys Ser Phe Ser Asp Asn Thr     290 295 300 Tyr Thr Thr Trp Gly Asn Arg Leu Ile Asn Gly Ser Asn Gly Ile Ala 305 310 315 320 Thr Thr Ser Arg Ile Ala Ser Val Tyr Thr Gly Thr Ser Ser Gly Gly                 325 330 335 Ser Ser Ser Ser Ser Ser Ser Ser Asn Ser Ser Ser Ser Gly Gly Thr             340 345 350 Ser Ala Cys Ser Thr Gly Gly Ser Cys Asp Trp Ser Gly Thr Ser Phe         355 360 365 Pro Leu Cys Glu Asn Gly Asn Thr Asn Asp Trp Gly Tyr Glu Asn Gly     370 375 380 Gln Ser Cys Val Gly Val Gly Leu Cys Asp Ser Asn Pro Asp Ala Thr 385 390 395 400 Thr His Cys Gly Thr Ser Ser Gly Gly Ser Thr Ser Cys Gly Thr                 405 410 415 Thr Ser Asp Gly His Pro Val Cys Cys Asp Ala Ala Ser Asp Pro Asp             420 425 430 Gly Asp Gly Trp Gly Trp Glu Asn Glu Ala Ser Cys Val Val Glu Ser         435 440 445 Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser     450 455 460 Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly Gly Ser Ser Ala Thr Cys 465 470 475 480 Asn Trp Tyr Gly Thr Gln Tyr Pro Met Cys Thr Ser Thr Ser Ser Gly                 485 490 495 Trp Gly Trp Glu Asn Asn Gln Ser Cys Ile Ser Pro Ser Thr Cys Ser             500 505 510 Gly gln          <210> 149 <211> 52 <212> PRT <213> Microbulbifer degradans <400> 149 Met Glu His Ala Asp Val Pro Pro Leu Leu Leu Glu Leu Asp Asp Asp 1 5 10 15 Glu Glu Leu Glu Leu Pro Pro Leu Asp Val Pro Val Tyr Thr Leu Ala             20 25 30 Ile Arg Glu Val Val Ala Ile Pro Leu Leu Pro Phe Ile Lys Arg Leu         35 40 45 Pro Gln Val Val     50 <210> 150 <211> 1545 <212> DNA <213> Microbulbifer degradans <400> 150 atgaataacg aggaagtcaa catgagaata ttttctatag cgcttgcgct atgcgccgtg 60 ctttgcgcgg gtcaatcttt agctgggtta tctattcagg gtacgcgact tgtggatggc 120 aatggcagca cggttgtgct gcgcggcgtg aaccatcccc atgcgtggta tgccggtgaa 180 acggcggcgg ctatccctaa aattgctgca acaggtgcta actcggtacg ggtagtgatg 240 gctatgggca ctaagtggag ccgcaccagt gccgcagaga ttcaaaccat tattgacctg 300 tgcaaacaaa acaacatgat tgctgtactg gaatttcacg atggcactgg ctggggggaa 360 gagagcggta ctgcgcatat atctgatatt gccgattact gggtgagcag tgatgtaatg 420 gcggtagtta agggtgagga agactacgta attataaata ttgccaacga accctttggc 480 aacggtgtat cggcaagcac ctacaccaac gacacgattg cggctattca aaaactgcgc 540 aacgccggtt atacccacac ccttatggtt gatgcggcaa attgggggca agattggcag 600 aacctaatgc gtgataacgc acaaaccatt tttaatggcg actcacttaa caacactatg 660 tttagcgtgc atatgtatca ggtgtataac acctcggcaa aagtgcaaag ctacatgcaa 720 gcctttagcg acaaaggttt agcgttagtt gtaggcgaat tcgcagcaga tcattttagt 780 gaagatgttg cagaagcagc cattatgcaa tacgccgagc aattcggttt tggttacatg 840 ggttggagtt ggacgggtaa tagctcggat ttagcatcgc tagacatagt aaaaagcttt 900 agcgataaca cctatacaac ctggggcaac cgcttaataa acggcagcaa cggtattgcg 960 accacttcgc gtatagccag tgtgtacaca ggaacgtcta gcggcggcag ctctagttct 1020 tcgtcatcgt ctaattcaag tagcagtggc ggtacatcag cgtgttccac tggcggaagt 1080 tgtgattgga gtggtacaag ctttccattg tgtgaaaacg gtaatactaa cgactggggt 1140 tacgagaatg gccaaagttg tgtaggtgta ggtttatgcg attctaaccc cgatgctacc 1200 actcactgcg gtacttcttc tggcggcggt tctacaagtt gcggcactac cagcgacggc 1260 caccctgttt gctgtgatgc agcctcagac ccagatggcg atggctgggg ttgggagaac 1320 gaagccagtt gtgtcgttga aagcggtagc tcttcgagtt catcaagttc tagctctacg 1380 tctagttcaa gttcgtcgag ctcttcaagc agctcaagtg gcggtagcag cgccacgtgt 1440 aattggtatg gcactcaata cccaatgtgt acttctacca gtagcggctg ggggtgggag 1500 aataaccaaa gttgtatttc gccttctacg tgtagtgggc aatga 1545 <210> 151 <211> 457 <212> PRT <213> Microbulbifer degradans <400> 151 Met Val Asn Tyr Leu Lys Arg Ala Ala Leu Cys Met Leu Ala Leu Val 1 5 10 15 Ala Val Ala Cys Ala Lys Ser Gln Pro Glu Thr Lys Val Glu Glu Leu             20 25 30 Pro Ala Thr Lys Thr Ala Ala Asn Ala Gly Val Ala Ala Thr Glu Phe         35 40 45 Val Gln Val Asn Gly Gly Arg Phe Thr Leu Arg Gly Gln Asp Tyr Ala     50 55 60 Tyr Ile Gly Thr Asn Met Trp Phe Ala Ala Tyr Ile Gly Ser Thr Asn 65 70 75 80 Pro Glu Tyr Gly Asp Arg Glu Arg Leu Ile Lys Glu Leu Asp Leu Leu                 85 90 95 Lys Ser Leu Gly Val Thr Asn Leu Arg Ile Leu Gly Ala Ser Glu Lys             100 105 110 Ser Pro Leu Arg Asp Ser Met Lys Pro Ala Ile Ser Glu Arg Gly Glu         115 120 125 Ile Asn Gln His Asp Ile Leu Glu Gly Leu Asp Phe Ala Leu Ala Glu     130 135 140 Met Ala Lys Arg Asp Met Lys Ala Val Ile Phe Leu Asn Asn Phe Trp 145 150 155 160 Glu Trp Ser Gly Gly Met Ala Thr Tyr Leu Ser Trp Val Asn Gly Gly                 165 170 175 Glu Ile Val Asp Met Ala Asp Pro Thr Lys Pro Trp Pro Ala Phe Ala             180 185 190 Leu Phe Ser Ala Gly Phe Tyr Ser Asn Glu Glu Ala Lys Gln Leu Phe         195 200 205 Asn Asn Tyr Leu Thr Lys Val Val Ser Arg Arg Asn Thr Ile Thr Gly     210 215 220 Glu Leu Tyr Ala Asn Asp Pro Thr Ile Met Ser Trp Gln Leu Ala Asn 225 230 235 240 Glu Pro Arg Pro Gly Asn Gly Asp Val Ser Lys Ser Asn Leu Pro Ala                 245 250 255 Tyr Tyr Asp Trp Ile Ser Lys Thr Thr Gln Leu Ile Lys Ser Ile Ala             260 265 270 Pro Lys Gln Leu Val Ser Ile Gly Ser Glu Gly Thr Met Gly Cys Leu         275 280 285 Glu Leu Asp Glu Cys Val Ile Thr Ala His Lys Glu Thr Gly Ile Asp     290 295 300 Tyr Val Thr Phe His Met Trp Leu Lys Asn Trp Gly Trp Phe Asp Val 305 310 315 320 Gln Asn Ala Glu Gln Thr Tyr Asp Ser Ala Val Ala Thr Ala Asp Lys                 325 330 335 Tyr Ile Asp His His Ile Lys Leu Ala Asn Glu Leu Asn Met Pro Val             340 345 350 Val Leu Glu Glu Phe Gly Met Glu Arg Asp Gly Gly Glu Phe Ser Pro         355 360 365 Glu Ser Ala Val Thr Tyr Arg Asp Lys Phe Tyr Ala Tyr Val Phe Asp     370 375 380 Arg Gln Ile Lys Ser Ile Arg Ser Gly Gly Pro Phe Val Gly Ser Asn 385 390 395 400 Phe Trp Ala Trp Gly Gly Tyr Gly Lys Ala Met His Asp Asp Ala Val                 405 410 415 Trp Arg Lys Gly Asp Lys Thr Phe Val Gly Asp Pro Pro Gln Glu Pro             420 425 430 Gln Gly Leu Asn Ala Val Phe Ala Ser Asp Thr Ser Thr Leu Glu Val         435 440 445 Leu Lys Gln Ala Ala Asp Ala Ile Arg     450 455 <210> 152 <211> 1374 <212> DNA <213> Microbulbifer degradans <400> 152 atggtgaact acttaaagcg cgctgccctg tgcatgcttg cgctcgttgc tgtggcttgc 60 gccaaatcgc agccagaaac aaaagttgaa gagctgcctg ccaccaaaac tgcggcaaat 120 gccggtgtgg cagcgacaga gtttgtgcaa gtaaatggtg gccgctttac attgcgcggg 180 caggactacg cctacatagg cactaatatg tggtttgccg cgtatattgg ttctaccaac 240 cctgaatacg gcgatcgcga gcgcctaatt aaagaactcg atttattaaa atccttgggt 300 gtgaccaacc tgcgtatttt aggagcatcc gaaaaatctc cattgcgcga ttccatgaaa 360 ccggcaatta gtgagcgcgg tgaaattaac cagcacgata ttttagaggg cttagatttt 420 gcgttagctg aaatggctaa gcgcgatatg aaagccgtga tatttctgaa taacttttgg 480 gaatggtccg gcggtatggc gacttaccta agctgggtta acggtggcga aattgttgat 540 atggcagacc ccaccaaacc atggccagct tttgcacttt tttcagcggg gttttactct 600 aatgaagagg cgaaacaact attcaataat taccttacaa aagttgtaag ccgccgcaat 660 accattaccg gcgagttgta cgctaacgac cccactatta tgtcttggca gctcgctaac 720 gagccgcgcc caggtaacgg cgatgtgagc aagtctaact tacccgccta ttacgattgg 780 ataagtaaaa ccactcagct aattaaaagc attgccccta agcagttggt gtcgattggt 840 agcgaaggta ccatgggctg cttagagttg gatgagtgcg ttattaccgc ccacaaagaa 900 acaggtatcg actacgttac attccatatg tggcttaaaa actggggctg gttcgatgtg 960 caaaacgcag agcaaaccta cgacagcgca gttgctactg cagataaata tatcgatcac 1020 cacattaaac tggctaacga gttaaatatg ccggtggtgc tggaagagtt tggtatggag 1080 cgcgacggag gtgaattctc cccagaaagc gcggtaacct atcgcgataa attctatgcc 1140 tatgtgttcg atcgccaaat taaaagtatt cgctctggcg ggccgtttgt agggtccaac 1200 ttttgggcgt ggggcggtta tggcaaagcc atgcacgatg atgctgtatg gcgcaaaggc 1260 gataaaacct ttgttggtga cccgccgcag gagccgcaag gtttaaatgc ggtatttgct 1320 tccgatactt caacgctgga agtgttgaag caagcggcgg acgctattcg gtaa 1374 <210> 153 <211> 850 <212> PRT <213> Microbulbifer degradans <400> 153 Met Asn Thr Lys Ile Lys Leu Leu Ser Phe Leu Ala Ser Ala Met Leu 1 5 10 15 Leu Gln Ala Cys Gly Gly Asp Met Leu Gly Thr Ser Asp Ser Glu Asp             20 25 30 Tyr Lys Leu Ile Pro Glu Glu Val Thr Glu Asp Pro Thr Lys Ala Arg         35 40 45 Pro Ser Glu Asn Ala Pro Val Leu Lys Thr Ser Gly Thr Thr Ile Gln     50 55 60 Leu Pro Asp Gly Thr Pro Val Leu Leu Arg Gly Ile Asn Leu Gln Phe 65 70 75 80 Gly Asp Asn Pro Ile Glu Gln Ile Asp Gly Ile Gln Ala Ile Arg Glu                 85 90 95 Thr Gly Ser Asn Val Val Arg Ile Gln Leu Leu Ala Asp Thr Ser Thr             100 105 110 Ala Asn Leu Glu Ala Val Leu Asn Lys Val Val Glu His Asn Leu Ile         115 120 125 Ala Val Leu Ser Leu Tyr Asp Glu Ala Leu His Cys Lys Glu Asp Asp     130 135 140 Glu Ala Phe Thr Asp Ala Val Lys Gln Val Trp Leu Thr Asp Phe Leu 145 150 155 160 Pro Ile Ile Ala Gln Asp Arg Tyr Gln Ala Asn Ile Met Ile Asn Ile                 165 170 175 Ala Ser Gly Trp Gly Pro Glu Ala Ile Phe Asp Gly Tyr Ser Val Gly             180 185 190 Tyr Lys Thr Tyr Thr Asp Asn Tyr Lys Ala Ala Ile Arg Gln Phe Arg         195 200 205 Lys Ala Gly Phe Asn Val Pro Leu Val Ile Asp Ala Pro Gly Cys Gly     210 215 220 Ala Asp Phe Asn Ala Phe Leu Ser Asn Arg Gly Lys Glu Leu Met Ala 225 230 235 240 Ala Asp Asp Lys Asp Asn Leu Val Leu Ser Val His Gly Tyr Gly Ser                 245 250 255 Gln Trp Asn Thr Ala Thr Lys Val Thr Asp Ala Ile Ser Gln Leu Ala             260 265 270 Ala Gln Asn Ile Pro Val Leu Met Ser Glu Phe Gly Gly Ser Gly Val         275 280 285 Gly Glu Lys Pro Val Lys His Met Ala Ile Leu Asp Lys Gly Ala Gly     290 295 300 Asp Tyr Ala Ala Glu Ile Ile Leu Pro Trp Ala Ser Ala Thr Asp Lys 305 310 315 320 Val Ala Met Asn Val Pro Phe Ser Ala Pro Ile Asn Leu Thr Asn Thr                 325 330 335 Asp Val Ser Phe Asp Val Lys Leu Asp Glu Ala Tyr Val Thr Asp Gly             340 345 350 Gln Met Gly Val Val Met Tyr Leu Arg Asp Val Asn Gly Glu Tyr Ala         355 360 365 Asn Leu Ala Trp His Ser Ala Ser Glu Phe Pro Ala Gly Glu Trp Ala     370 375 380 Thr Lys Ser Tyr Ala Ile Gln Asn Asn Ala Ser Phe Gly Trp Ala Ser 385 390 395 400 Glu Ala Phe Asp Ile Thr Ala Val Ala Lys Val Gly Val Glu Leu Val                 405 410 415 Ala Asn Gly Lys Leu Ala Glu Val Ala Gly Ser Val Val Ile Asp Asn             420 425 430 Leu Arg Val Ala Glu Gly Ser Gly Ala Val Glu Leu Tyr Ser Gln Ser         435 440 445 Phe Asp Asp Asp Ile Ala Gly Trp Gly Val Pro Trp Thr Gly Thr Val     450 455 460 Ala Ala His Ala Asp Gly Ala Leu Ser Leu Thr His Asp Asn Gly Glu 465 470 475 480 Ile Ile Ala Gln Leu Asp Gly Leu Gly Gly Val Val Asp Phe Ala Gln                 485 490 495 Pro Val Val Ile Ser Gly Arg Phe Phe Val Pro Ala Asp Tyr Ala Gly             500 505 510 Ser Trp Met Tyr Ala Lys Phe Phe Asn Asn Gly Glu Ala Trp Thr Glu         515 520 525 Val Gly Ile Thr Gly Leu Thr Pro Gly Glu Trp Thr Glu Ile Ser Val     530 535 540 Glu Thr Glu Phe Pro Ala Ala Ala Thr Ser Val Gly Ile Gln Ile Gly 545 550 555 560 Asn Ile Gly Ile Ala Asp Gly Ala Thr Ile Thr Asp Ser Thr Glu Pro                 565 570 575 Phe Leu Leu Asp Asp Phe Ala Ile Ser Gly Val Ala Ala Asn Asp Ser             580 585 590 Phe Glu Leu Gly Thr Gln Tyr Met Ala Ser Phe Asp Glu Ser Glu Asp         595 600 605 Gly Trp Ala Tyr Leu Ser Trp Gly Ala Ser Ala Thr Val Glu Ala Ile     610 615 620 Asp Gly Ala Leu Asn Phe Tyr Pro Asn Ala Asn Asp Ala Val Arg Ile 625 630 635 640 Val Leu Tyr Lys Thr Asp Leu Ser Ala Ile Asp Asp Leu Asp Leu Gln                 645 650 655 Asp Pro Phe Thr Ile Lys Thr Arg Val Leu Ile Pro Asp Ser Tyr Thr             660 665 670 Gly Gln Ala Phe Glu Tyr Gln Leu Phe Leu Gln Asp Ala Asn Trp Gln         675 680 685 Asn His Phe Ala Ala Lys Ile Trp Asn Gln Asp Glu Leu Ile Pro Gly     690 695 700 Glu Trp Met Asp Leu Val Val Glu Val Glu Phe Pro Ala Glu Phe Asp 705 710 715 720 Arg Ala Gly Ile Pro Gln Tyr Leu Gly Phe Asp Leu Ser Ser Glu Val                 725 730 735 Ala Leu Pro Gln Asp Pro Ile Leu Ile Asp Glu Ile Val Phe Glu Gly             740 745 750 Met Val Pro Val Glu Lys Glu Val Val Ile Ale Gln Val Asp Phe         755 760 765 Phe Tyr Thr Asn His Phe Thr Asp Phe Ala Ile Asp Tyr Ile Glu Gly     770 775 780 Glu Ile Leu Glu Asp Asp Ile Leu Glu Leu Ala Tyr Ile His Gln Arg 785 790 795 800 Ser Glu Pro Phe Ser Trp Ile Ala Trp Ser Trp Tyr Gly Asn Asp Ile                 805 810 815 Glu Asn Ser Asp Trp Asp Met Thr Thr Ile Val Gly Asp Ala Thr Ala             820 825 830 Leu Thr Glu Arg Gly Glu Asp Ile Val Asn Gly Lys Gly Gly Ile Ala         835 840 845 Gly asn     850 <210> 154 <211> 2553 <212> DNA <213> Microbulbifer degradans <400> 154 atgaacacaa aaataaaatt actttcattt cttgcgagtg caatgctgtt gcaggcatgt 60 ggtggtgaca tgctgggcac ctctgacagc gaagactata aattgattcc agaagaagta 120 acggaagatc caactaaagc taggccgtct gaaaatgccc ccgtgttaaa aacgagtggt 180 actactatac agctaccaga tggtacacca gtgctgctgc gaggtattaa ccttcaattt 240 ggcgacaatc ctattgagca aatagatggc atacaggcta ttcgcgaaac aggttcaaac 300 gttgtgcgaa ttcaattgtt agcagataca tctacagcaa atttagaggc ggtgctaaat 360 aaagttgttg agcataattt aatagctgta cttagtctct acgacgaagc tttacattgt 420 aaagaagatg atgaggcatt tacggatgca gttaagcaag tatggcttac cgattttctt 480 ccaattattg ctcaagatcg ttatcaagca aatattatga ttaacatcgc aagtggctgg 540 gggcctgaag ccatttttga tgggtatagt gtaggttaca aaacgtatac cgataactat 600 aaagcagcta ttcgtcagtt ccgaaaggct gggtttaatg tgcctttggt aattgatgcc 660 cctggatgtg gtgctgactt taacgctttt ttaagtaatc gcggtaaaga gcttatggct 720 gcggatgata aagacaatct tgtgttgtcc gtgcacggtt atggttcgca gtggaacacg 780 gctactaaag ttacggatgc aattagccag cttgctgcgc aaaatatccc tgtgctaatg 840 agcgagtttg gtggctctgg tgtcggtgaa aagcctgtta aacatatggc gatccttgat 900 aaaggtgcag gagattacgc cgcagaaatt attctgccat gggcgagcgc tacagataaa 960 gtggctatga atgtgccatt ctcagcacct atcaatctga caaacactga tgtgagcttt 1020 gacgttaagc tggatgaagc atatgtcacg gatggtcaaa tgggggttgt aatgtacctc 1080 cgagatgtaa atggagaata tgcaaactta gcttggcact ctgcctctga atttccggct 1140 ggtgaatggg ctactaaatc ttatgcgatt cagaataatg catctttcgg ctgggcaagt 1200 gaggcgtttg atattactgc cgtcgctaaa gttggtgttg agttggttgc aaatggcaag 1260 ctcgccgagg ttgctggcag cgttgttatc gacaaccttc gagtggctga aggtagtggt 1320 gcagtcgagt tatacagtca aagctttgat gatgatattg caggttgggg cgtgccttgg 1380 actggcacag ttgctgcgca cgctgacggt gctttatcgc taacccatga caatggagaa 1440 attattgccc agctggacgg gcttggtggt gtagttgatt ttgcccagcc ggttgttatt 1500 agtggcaggt tttttgtgcc tgcggattat gctggttcat ggatgtacgc taaatttttc 1560 aataacggcg aggcatggac agaggttgga attactgggc ttactcctgg agagtggaca 1620 gaaatttccg tcgaaaccga atttcctgca gctgctacca gtgtaggcat tcagattggc 1680 aacataggta ttgctgatgg ggcaaccatt acagattcca cagagccttt tttgctggac 1740 gactttgcta taagtggcgt agcagctaat gactcgttcg aacttggtac gcagtatatg 1800 gcgtcgttcg atgaatctga agatggctgg gcttacttga gttggggggc gtctgctact 1860 gtagaggcca ttgatggcgc tttgaatttc tatccaaatg ccaatgatgc ggtgagaata 1920 gtactttata aaaccgactt aagtgctata gacgacttgg atttgcagga cccgttcacc 1980 attaaaacgc gagtattgat tccagatagc tacacaggac aggcgttcga gtatcagctg 2040 tttctacaag atgctaactg gcaaaatcat tttgcagcga aaatttggaa ccaagatgag 2100 cttatccctg gtgagtggat ggacttggtg gttgaggttg agtttcctgc tgaattcgat 2160 cgggctggaa ttcctcaata cctcggtttt gacctgtcct ccgaagttgc tttaccacaa 2220 gacccaatac taattgatga aatagttttt gaaggcatgg ttccagtaga aaaagaagtt 2280 gtgatcattg atcaggtaga tttcttctac actaaccact ttacagattt tgctatcgac 2340 tatattgagg gtgaaatatt agaggacgac attctggagc ttgcttatat tcatcagcgc 2400 agcgagccat tctcttggat agcttggtct tggtacggaa atgatatcga aaattctgac 2460 tgggatatga ccaccatagt tggcgacgca acagccttga ctgaacgtgg tgaagatatc 2520 gtaaatggta aaggtgggat tgcaggtaac tag 2553 <210> 155 <211> 408 <212> PRT <213> Microbulbifer degradans <400> 155 Met Gln Lys Ile Thr Arg Leu Ala Ala Leu Val Val Ala Gly Leu Cys 1 5 10 15 Leu Leu Gly Thr Gln Ala Gln Ala Lys Lys Phe Glu His Leu Ala Gln             20 25 30 Thr Pro Pro Met Gly Trp Asn Ser Trp Asn Asn Phe Gly Cys Asp Val         35 40 45 Asp Glu Lys Leu Ile Lys Glu Thr Ala Asp Tyr Met Val Ser Ser Gly     50 55 60 Met Lys Asp Ala Gly Tyr Glu Tyr Val Asn Ile Asp Asp Cys Trp His 65 70 75 80 Gly Glu Arg Asp Ala Asn Gly Phe Ile Gln Ala Asp Pro Glu Arg Phe                 85 90 95 Pro Ser Gly Ile Lys Ala Leu Ala Asp Tyr Val His Ser Lys Gly Leu             100 105 110 Lys Phe Gly Ile Tyr Ser Asp Ala Gly Trp Thr Thr Cys Gly Gly Lys         115 120 125 Pro Gly Ser Arg Gly Tyr Glu Phe Gln Asp Ala Gln Met Tyr Ala Lys     130 135 140 Trp Gly Val Asp Tyr Leu Lys Tyr Asp Trp Cys Ala Thr Asp Gly Leu 145 150 155 160 Lys Ala Glu Gly Ala Tyr Gln Thr Met Arg Glu Ala Ile His Lys Ala                 165 170 175 Gly Arg Pro Met Val Phe Ser Ile Cys Glu Trp Gly Asp Asn Gln Pro             180 185 190 Trp Glu Trp Ala Lys Pro Ile Gly His Leu Trp Arg Thr Thr Gly Asp         195 200 205 Ile Tyr Asn Cys Phe Asp Cys Glu Tyr Asp His Gly Thr Trp Ser Ser     210 215 220 Trp Gly Val Leu Gln Ile Leu Asp Met Gln Asp Asp Leu Arg Gln Tyr 225 230 235 240 Ala Gly Pro Gly His Trp Asn Asp Pro Asp Met Met Glu Val Gly Asn                 245 250 255 Gly Met Thr Glu Ala Glu Asp Arg Ser His Phe Ser Met Trp Ala Met             260 265 270 Leu Ala Ala Pro Leu Ile Ala Gly Asn Asp Ile Arg Asn Met Ser Glu         275 280 285 Ala Thr Arg Lys Ile Leu Thr Asn Lys Ala Val Ile Ala Val Asp Gln     290 295 300 Asp Glu Leu Gly Val Gln Gly Phe Lys Tyr Ser Ser Lys Asn Gly Val 305 310 315 320 Glu Val Trp Phe Lys Pro Leu Ala Asn Asp Glu Trp Ala Met Ala Val                 325 330 335 Leu Asn Arg Asn Lys Gly Glu Val Lys Phe Glu Phe Lys Trp Arg Asn             340 345 350 Glu Val Val Lys Asp Glu Leu Thr His Arg Thr Ile Thr Phe Asn Glu         355 360 365 Gln Lys Phe Asp Trp Gln Asp Leu Trp Asn Lys Ser Asn Lys Gly His     370 375 380 Thr Lys Lys Phe Leu Lys Thr Lys Ile Ala Gly His Asp Thr Leu Met 385 390 395 400 Phe Arg Leu Thr Pro Ala Lys Asn                 405 <210> 156 <211> 1227 <212> DNA <213> Microbulbifer degradans <400> 156 atgcaaaaaa ttacacgttt agcggcgtta gtcgtcgctg gcttgtgcct gctaggtacg 60 caggcgcagg cgaaaaagtt tgaacactta gcccaaaccc cgcctatggg gtggaatagc 120 tggaacaatt ttggctgtga tgtagatgaa aagctcatca aggaaacggc tgattatatg 180 gtttcttccg gcatgaaaga cgcgggatat gagtatgtaa acatagacga ttgctggcat 240 ggtgagcgcg atgctaatgg ttttattcag gcagaccccg agcggtttcc ttccggtatt 300 aaagcgcttg ccgactatgt tcattcgaag gggctgaagt ttggtattta ttctgacgcg 360 ggttggacca cctgcggcgg taaaccgggc agtcgtgggt acgagtttca agatgcacaa 420 atgtatgcga aatggggcgt ggattatctt aaatacgatt ggtgcgctac agatggctta 480 aaggccgaag gcgcatatca aacaatgcgt gaagccattc ataaagccgg tagaccaatg 540 gtatttagca tttgcgagtg gggcgacaac cagccttggg agtgggccaa acctattggg 600 catctatggc gtaccacggg tgatatctac aactgtttcg attgcgaata tgaccacggt 660 acttggtctt cctggggtgt attgcaaatt ttggatatgc aagacgacct aaggcagtac 720 gctgggccag gccattggaa cgaccccgat atgatggagg tgggcaatgg catgactgaa 780 gctgaagacc gttcgcattt ttcaatgtgg gctatgttgg cagcgcccct tattgctggt 840 aacgatatac gcaatatgtc ggaagctacc agaaaaattc tcaccaataa ggccgtgata 900 gcagtcgatc aggacgagct aggcgtacag ggctttaaat attccagtaa aaatggcgta 960 gaggtttggt ttaaaccgtt ggcgaatgat gagtgggcaa tggctgtact caaccgaaat 1020 aaaggtgaag ttaaattcga gtttaagtgg cgtaatgaag tggttaaaga tgagctgacg 1080 catcgcacta ttacgttcaa cgagcaaaaa tttgactggc aagatttgtg gaataaatcc 1140 aacaagggcc ataccaaaaa attcctaaaa acaaaaatag ctgggcacga tacgctgatg 1200 tttaggctaa cgccggctaa aaactag 1227 <210> 157 <211> 1733 <212> PRT <213> Microbulbifer degradans <400> 157 Met Ser Cys Thr Pro Cys Ile Arg Ser Lys Ala Ser Ser Leu Asn Phe   1 5 10 15 Asp Asn Lys Leu Ile Lys Ser Glu Phe Ile Met Ser Asn Gln Lys Arg              20 25 30 Leu Ser Ala Gly Leu Ile Ala Lys Leu Ala Leu Ala Ser Gly Leu Thr          35 40 45 Leu Val Ala Thr Ala Ala Thr His Ala Asp Val Thr Asn Pro Val Ile      50 55 60 Gly Asn Leu Ile Phe Glu Glu Asn Phe Asn Thr Leu Asn Ser Asp Arg  65 70 75 80 Trp Asn Val Ile Glu Gly Asp Gly Cys Gln Tyr Gly Pro Asn Leu Cys                  85 90 95 Gly Trp Gly Asn Gln Glu Leu Gln Tyr Tyr Gln Asp Ser Asn Val Thr             100 105 110 Ile Glu Asp Val Pro Gly Glu Pro Gly Asn Lys Ala Val Val Phe Glu         115 120 125 Ala Arg Asn Glu Thr Val Asn Gly Ser Ala Phe Thr Ser Gly Lys Ile     130 135 140 Asp Ser Glu Asp Gly Ile Ala Ile Lys Tyr Gly Met Ile Glu Phe Arg 145 150 155 160 Leu Arg Val Pro Asn Met Gly Val Gly Tyr Trp Pro Ala Val Trp Met                 165 170 175 Leu Gly Thr Ser Thr Glu Ser Trp Pro Ser Lys Gly Glu Ile Asp Met             180 185 190 Met Glu Met Gly His Arg Ala Gln Gly Met Ala Asp Ala Gly His Pro         195 200 205 Gly Thr Asn Leu Asn Asn Tyr Thr Ala Ala Asn Ala Ile Phe Tyr Ala     210 215 220 Glu Ala Ala Cys Val Pro Glu Asn Pro Thr Cys Ala Ala Met Thr Ala 225 230 235 240 Trp Gln Thr Asp Asn Ala Tyr Val Ser Gln Thr Ser Met Gly Glu Arg                 245 250 255 Phe Val Ile Tyr Arg Thr Tyr Trp Thr Asp Thr Gln Leu Arg Phe Thr             260 265 270 Val Ile Asp Asn Gly Val Glu Tyr Asp Met Tyr Asp Asp Pro Ile Thr         275 280 285 Ile Gly Glu Glu Ala Thr Glu Leu Gln Gln Pro Phe Tyr Leu Leu Ala     290 295 300 Asn Leu Ala Val Gly Gly Asn Phe Thr Asp Ala Ser Thr Pro Ala Glu 305 310 315 320 Val Thr Ala Gln Leu Pro Gly Lys Met Tyr Leu Asp Tyr Ile Arg Val                 325 330 335 Tyr Gln Leu Asp Gly Met Gly Glu Ile Phe Glu Gly Ser Ile Ala Gln             340 345 350 Lys Glu Tyr Gly Thr Phe Gly Val Phe Thr Asp Asp Thr Pro Thr Ser         355 360 365 Asn Lys Leu Val Ala Gly Asp Thr Ser Gln Ile Tyr Ile Trp Asn Gln     370 375 380 Asn Ser Leu Ser Glu Gly Thr Leu Ala Pro Ala Glu Gly Asp Asn Val 385 390 395 400 Ile Ala Trp Asn Tyr Thr Ala Pro Glu Trp Phe Gly Ala Gly Ile Gln                 405 410 415 Ala Val His Ala Arg Asp Met Ser Asn Phe Glu Asn Gly Glu Phe Lys             420 425 430 Phe Lys Ile Lys Ile Pro Ala Asn Val Ser Phe Lys Val Gly Phe Ala         435 440 445 Asp Thr Tyr Thr Asn Glu Asn Trp Leu Thr Phe Pro Ala Asn Gln Thr     450 455 460 Thr Tyr Gly Leu Val Arg Asn Gly Glu Trp Ala Glu Ala Thr Ile Pro 465 470 475 480 Val Ala Asp Leu Arg Gly Ser Leu Ile Ala Leu Gln Ser Met Ala Gly                 485 490 495 Met Phe Tyr Ile Ala Ser Val Asp Gly Gln Ile Pro Thr Ser Asn Phe             500 505 510 Glu Phe Ala Ile Asp Asp Val Arg Trp Glu Gly Gly Gly Ala Gly Pro         515 520 525 Val Asp Ser Asp Gly Asp Gly Val Ala Asp Glu Leu Asp Gln Cys Pro     530 535 540 Asn Thr Pro Ala Gly Thr Ala Val Asp Ser Val Gly Cys Gln Ile Gly 545 550 555 560 Leu Pro Gln Pro Val Ala Val Thr Val Glu Ala Glu Asp Tyr Glu Ala                 565 570 575 Tyr Tyr Asp Thr Thr Ser Gly Asn Ser Gly Asn Ala Tyr Arg Ser Asp             580 585 590 Asp Val Asp Ile Glu Ala Ala Ser Glu Gly Gly Phe Asn Val Gly Trp         595 600 605 Thr Asp Ala Gly Glu Trp Met Asp Tyr Thr Leu Asn Leu Ala Ala Gly     610 615 620 Thr Tyr Asp Val Thr Ala Arg Val Ala Ser Asn Thr Asp Thr Gly Val 625 630 635 640 Tyr Ser Val Ser Leu Asp Gly Thr Thr Ile Gly Ser Asn Gly Val Ala                 645 650 655 Thr Gly Gly Trp Gln Asn Trp Val Thr Gln Val Val Gly Gln Ile Thr             660 665 670 Val Asn Gly Gly Gln Gln Thr Leu Arg Ile Ser Thr Asp Val Ala Gly         675 680 685 Tyr Asn Ile Asn Trp Val His Phe Glu Pro Val Pro Asp Ala Asp Asn     690 695 700 Asp Gly Val Pro Asp Ser Gln Asp Asn Cys Pro Asn Thr Pro Ala Gly 705 710 715 720 Thr Glu Val Asp Ala Asn Gly Cys Ala Ile Val Val Asp Pro Val Pro                 725 730 735 Val His Ile Glu Ala Glu Asp Tyr Ala Ala Tyr His Asp Leu Ser Ala             740 745 750 Gly Asn Asn Gly Gly Gln Tyr Arg Ser Asp Asp Val Asp Ile Glu Ala         755 760 765 Ala Ser Glu Gly Gly Phe Asn Val Gly Trp Thr Asp Thr Gly Glu Trp     770 775 780 Leu Glu Tyr Ser Val Glu Leu Ile Glu Gly Val Tyr Asp Leu Thr Ala 785 790 795 800 Arg Val Ala Ser Leu Ser Gly Asn Gly Ala Tyr Ser Val Ser Ile Ser                 805 810 815 Gly Gln Ala Val Gly Gly Ser Asn Ala Val Ala Thr Gly Gly Trp Gln             820 825 830 Asn Trp Glu Thr Gln His Val Ala Arg Phe Val Ala Gly Thr Gly Thr         835 840 845 Tyr Thr Ile Arg Val Asn Ala Asp Ala Gly Gly Phe Asn Leu Asn Trp     850 855 860 Leu His Leu Glu Pro Val Asn Asp Pro Asp Ser Asp Asn Asp Gly Val 865 870 875 880 Pro Asp Ser Gln Asp Asn Cys Ala Asn Thr Pro Ala Gly Thr Glu Val                 885 890 895 Asp Ala Ser Gly Cys Pro Val Val Val Gln Pro Phe Gly Val Thr Gln             900 905 910 Ser Asp Ser Asn Ser Ala Gln Phe Tyr Val Asn Gly Ala Asp Trp Ala         915 920 925 Val Leu His Tyr Ser Val Asn Gly Gly Gly Gln Val Asn Val Ser Met     930 935 940 Ser Leu Glu Asn Gly Lys His Val Tyr Thr Val Pro Asp Leu Ala Pro 945 950 955 960 Gly Asp Ser Ile Ser Tyr Phe Val Thr Tyr Trp Asp Pro Glu Leu Gly                 965 970 975 Gly Ala Arg Asp Ser Glu Thr Val Ser Tyr Ser Val Val Ala Ala Gly             980 985 990 Ser Asp Ser Asp Gly Asp Gly Val Gly Asp Ser Ala Asp Gln Cys Pro         995 1000 1005 Asn Thr Pro Ala Gly Thr Ala Val Asp Ser Val Gly Cys Pro Val Thr    1010 1015 1020 Gln Pro Pro Ser Asp Ser Asp Asn Asp Gly Val Thr Asp Ala Asn Asp 1025 1030 1035 1040 Gln Cys Pro Asn Thr Pro Ala Gly Thr Ser Val Asp Ser Val Gly Cys                1045 1050 1055 Pro Val Val Gln Pro Pro Ser Asp Ser Asp Asn Asp Gly Val Asp Asp            1060 1065 1070 Ser Ser Asp Gln Cys Pro Asn Thr Pro Ala Gly Thr Ser Val Asn Ala        1075 1080 1085 Val Gly Cys Pro Val Thr Gln Thr Asn Ile Val Pro Leu Tyr Asn Ala    1090 1095 1100 Ser Thr Asn Leu Glu Gly Ala Ile Ser Phe Asp Arg Gly Asp Ala Leu 1105 1110 1115 1120 Val Thr Arg Ile Ser Asp Arg Gly Arg Asp Arg His Ala Lys Glu Asn                1125 1130 1135 His Phe Gln Ala Tyr Asp His Tyr Leu Thr Phe Tyr Trp Glu Asp Arg            1140 1145 1150 Thr Ile Ala Ile Glu Ile Val Asp Tyr Val Ala Lys Gly Gly Ser Ser        1155 1160 1165 Ile Arg Met Asn Ile Val Ser Gln Met Arg Leu Asp Asp Thr Glu Ala    1170 1175 1180 Glu Asn Arg Trp Phe Tyr Ile Gly Asn Asn Thr Leu Ala Glu Tyr Cys 1185 1190 1195 1200 Gly Asn Gly Val Met Asn Glu Val Asp His Thr His Tyr Trp Lys Glu                1205 1210 1215 Ser Ser Phe Asn Cys Arg Glu Gly Arg Pro Ile Gln Ile Gly Asp Lys            1220 1225 1230 Met Glu Phe Glu Ile Ser Gln Phe Leu Asp Pro Ala Leu Leu Pro Arg        1235 1240 1245 Gly Arg Ser Asn Tyr Tyr Gly Thr Thr Tyr Leu Tyr Ile Val Gly Glu    1250 1255 1260 Gly Leu Val Pro Trp Asp Val Thr Asp Lys Val Ala Phe Gln Gly Gly 1265 1270 1275 1280 Asn Arg Leu Gln Arg Asp Ser Ile Pro Val Pro Glu His Ala Arg Leu                1285 1290 1295 Gly Gly Asp Thr Thr Leu His Val Gln Met Thr Ala Glu Pro Asp Gly            1300 1305 1310 His Phe Gln Gln Met Ala Thr Asn Leu Gly Phe Asp Asn Gly Gln Pro        1315 1320 1325 Phe Val Leu Gly Arg Arg Val His His Thr Ser Tyr Val Asp Gly Thr    1330 1335 1340 His Asp Glu Ser Ala Glu Asn Gly Val Phe Asp Gly Met Pro Gly Lys 1345 1350 1355 1360 Ala Gly Pro His Tyr Val Asn Asp Arg Cys Ser Asp Cys His Glu Arg                1365 1370 1375 Asn Gly Arg Ala Pro Val Val Gly Ile Gly Glu Pro Leu Asp Arg Trp            1380 1385 1390 Val Phe Lys Val Gly Asp Ala Asn Gly Asn Pro His Pro Asp Met Gly        1395 1400 1405 Arg Val Leu Gln Pro Glu Ala Asn Asn Gly Ala Ala Ser Glu Gly Thr    1410 1415 1420 Pro Thr Ile Ala Phe Phe Ser Glu Glu Asn Gly Leu Arg Lys Pro Asn 1425 1430 1435 1440 Tyr Ala Phe Ser Gly Ile Thr Pro Asp Thr Phe Ser Ala Arg Ile Ala                1445 1450 1455 Pro Gln Leu Asn Gly Ile Gly Leu Leu Glu Ala Ile Pro Glu Ser Ala            1460 1465 1470 Ile Leu Ala Gln Glu Asp Val Asn Asp Ala Asn Gly Asp Gly Ile Ser        1475 1480 1485 Gly Lys Ala Gln Arg Ser Ile Asp Pro Val Thr Gly Glu Thr Arg Leu    1490 1495 1500 Gly Arg Phe Gly Tyr Lys Ala Ala Thr Ser Ser Val Lys His Gln Val 1505 1510 1515 1520 Ala Ala Ala Phe Asn Thr Asp Ile Gly Val Arg Thr Ser Val Met Pro                1525 1530 1535 Asn Pro Asp Cys Gly Ser Asn Gln Asn Asp Cys Gly Pro Ser Gly Ala            1540 1545 1550 Glu Leu Ala Asp Glu His Leu Asp Asn Leu Val Lys Tyr Val Ser Leu        1555 1560 1565 Leu Gly Val Arg Pro Gln Arg Asp Tyr Asn Asp Ala Gln Val Leu Gln    1570 1575 1580 Gly Lys Gln Val Phe Asn Asp Ala Gly Cys Val Ser Cys His Thr Asp 1585 1590 1595 1600 Thr Tyr Gln Thr Ser Gln Tyr His Pro Leu Ala Glu Leu Arg Ser Gln                1605 1610 1615 Thr Ile His Pro Tyr Thr Asp Leu Leu Leu His Asp Met Gly Pro Gly            1620 1625 1630 Leu Ala Asp Asn Leu Gly Glu Gly Asp Ala Thr Gly Ala Glu Trp Arg        1635 1640 1645 Thr Ala Pro Leu Trp Gly Leu Gly Leu Ser Ala Cys Val Thr Gly Gly    1650 1655 1660 Val Glu Gly Gly Arg Gly Trp Asp Asp Phe Gly Leu Asp Gly Tyr Glu 1665 1670 1675 1680 Thr Cys Thr Pro His His Ser Tyr Leu His Asp Gly Arg Ala Arg Thr                1685 1690 1695 Ile Glu Glu Ala Ile Leu Trp His Gly Gly Glu Gly Glu Asn Ser Lys            1700 1705 1710 Gln Ala Tyr Gln Asn Leu Ser Asn Ser Glu Arg Asp Ala Leu Leu Ala        1715 1720 1725 Phe Leu Asn Ser Leu    1730 <210> 158 <211> 5202 <212> DNA <213> Microbulbifer degradans <400> 158 gtgagttgta caccctgtat tagatcaaaa gcttcatcac ttaacttcga taataaactt 60 ataaaaagtg agttcatcat gagtaaccaa aagcgattgt ccgctgggtt aattgcaaag 120 ttagcgttgg cttcaggcct tacgctagtc gccactgcgg ccacccacgc cgacgttacc 180 aatcctgtta ttggtaattt aatctttgaa gagaatttca ataccctcaa cagcgatcgt 240 tggaatgtga ttgagggtga tggctgccaa tacggcccaa acctttgcgg ttggggcaac 300 caagaattgc agtattacca agatagcaat gtaaccatcg aagatgtgcc cggagaaccg 360 ggcaacaaag cggttgtttt tgaagcgcgc aacgaaactg taaatggcag tgcgtttaca 420 tccggcaaaa ttgattcgga agacggaata gccattaagt acggcatgat tgagttccgt 480 ttgcgagtac caaatatggg cgttggctat tggcctgcgg tttggatgct tggcacaagc 540 acagaaagct ggccgagcaa aggcgaaata gacatgatgg aaatgggcca tcgtgcccaa 600 ggcatggccg atgcgggcca cccaggcaca aacctaaaca actacaccgc ggccaatgca 660 attttttatg cagaagcagc ctgtgtaccc gaaaacccca cctgtgcagc catgaccgca 720 tggcaaacag ataacgccta tgtttctcaa acgtctatgg gcgagcgatt tgttatttac 780 cgcacttatt ggaccgacac ccagttgcgt tttaccgtaa ttgataatgg cgttgagtac 840 gatatgtacg atgatccaat tactatcggc gaagaggcta ctgagctgca acagccgttt 900 tatttgttgg caaacttagc agttggcggc aattttaccg atgcgtctac acctgccgaa 960 gttaccgcac agctgcccgg taaaatgtat ttagactata ttcgtgtgta ccaattagac 1020 ggtatgggcg aaatattcga aggctcaatt gcccaaaaag aatacggcac atttggtgtg 1080 tttaccgatg acacgcccac cagcaataag ctggtagccg gtgatacgtc acaaatttat 1140 atttggaatc aaaactccct aagtgaagga acgcttgcgc ccgccgaagg cgacaatgta 1200 atagcctgga actacaccgc gccagagtgg ttcggtgctg gcattcaagc tgtgcacgcg 1260 cgcgatatga gtaattttga gaatggcgag tttaaattca aaataaaaat acccgctaac 1320 gtctcattta aagttggttt tgcagatact tacaccaacg aaaactggct aactttccct 1380 gctaatcaaa caacctacgg tttggtgcgc aatggcgaat gggctgaggc gacaattcca 1440 gtagcggact tgcgcggcag tttaattgca ttgcagtcta tggctggtat gttttatatc 1500 gccagtgttg acggccaaat tccaacgtct aattttgaat ttgccatcga cgatgtgcgc 1560 tgggaaggcg gtggtgcagg gcctgtagac agcgacggcg acggcgtagc tgatgaactt 1620 gatcagtgcc ccaacacacc agccggtact gcagtagata gcgttggctg ccaaataggt 1680 ttgccacagc ctgtggctgt aacggtagaa gcggaagact acgaagctta ctacgataca 1740 accagtggca actctggcaa tgcgtatcgc agcgatgatg tagatataga agctgcaagc 1800 gaaggtggtt ttaacgtcgg ttggactgat gctggcgagt ggatggatta cactttaaac 1860 ctcgctgcgg gcacctacga cgttacagct cgtgttgctt caaataccga tactggtgtg 1920 tacagtgtta gtttagatgg caccacaatc ggctctaacg gagttgcaac tggcggttgg 1980 caaaactggg ttactcaagt tgtcggccaa attacggtaa atggcggtca acaaactctg 2040 cgcattagta ctgatgtggc tggatataac attaactggg tgcattttga gcccgtgcca 2100 gacgcagata acgatggcgt acctgacagc caagataact gccccaatac accagcgggt 2160 acagaggtgg atgctaatgg ctgtgcgatt gtcgtagacc cagtacccgt gcacatagaa 2220 gccgaagatt acgcggccta tcacgaccta tctgcaggta acaatggcgg tcaataccgc 2280 tctgatgatg tggatattga agccgctagc gaaggtggat tcaacgtagg ttggacagat 2340 actggcgagt ggttggaata ctcggttgaa ttaattgaag gtgtttatga cctaactgca 2400 cgcgtggcat cgttaagcgg caatggcgct tacagcgttt ctatttctgg tcaagctgtg 2460 ggtgggtcta atgctgtagc gactggtggt tggcaaaatt gggaaaccca gcacgtagcg 2520 cgttttgttg caggcacggg tacctacact attcgtgtaa atgcagatgc cggcggcttt 2580 aaccttaatt ggttgcactt agagccagta aacgaccccg atagcgataa cgacggtgtg 2640 ccagacagcc aagacaactg cgccaatacc cctgccggca ccgaagtgga cgccagcggt 2700 tgcccggtag ttgtacagcc atttggtgtg actcagtcag attccaacag cgcgcaattt 2760 tatgtaaatg gcgccgactg ggcagtgttg cattacagtg ttaatggcgg tgggcaagtg 2820 aatgtgtcca tgagtttgga aaacggtaag catgtatata ccgtgccaga cttggcccca 2880 ggtgactcca ttagttactt tgttacctat tgggacccag aactaggcgg tgcacgcgac 2940 agtgaaacgg taagctacag cgtagttgct gcgggtagcg atagtgatgg cgacggcgta 3000 ggtgatagtg ctgaccaatg cccaaataca cccgccggca cagctgtcga ttctgttggt 3060 tgcccagtaa cacagccacc atccgatagt gacaacgatg gcgttacgga tgctaatgat 3120 cagtgcccaa atacacctgc aggtacatcg gttgattccg ttggctgccc agtggttcag 3180 ccaccatcag atagcgataa cgacggtgtg gacgattcaa gtgatcaatg tccaaatacc 3240 cctgcgggta ccagtgtaaa tgcagtgggt tgccctgtaa cgcaaactaa tattgtgcct 3300 ttatataatg ccagcacaaa cttagaaggc gctatttctt ttgatcgcgg tgatgcgctt 3360 gttacgcgaa tttcagatcg cggtcgcgac cgtcacgcaa aagaaaacca cttccaagct 3420 tacgatcact accttacttt ctattgggaa gatcgcacta ttgctattga aatagtggat 3480 tacgttgcga aaggtggcag cagcattcgc atgaatatcg taagtcaaat gcgtttggat 3540 gacaccgaag cagaaaaccg ctggttttat attggtaaca acacgctcgc tgaatactgt 3600 ggcaatggcg tgatgaacga agtggatcac acgcactact ggaaggaatc tagctttaac 3660 tgtcgtgagg gtcgtcccat tcaaattggc gataaaatgg agtttgaaat cagccaattt 3720 ttagaccccg cgctattacc tcgcggtcgc tctaattatt acggcaccac ttacctttac 3780 attgtgggtg agggcttagt gccttgggat gttaccgata aagttgcttt ccaaggcggc 3840 aaccgtttgc agcgagattc cattcctgtg cccgagcacg cgcgtttagg tggcgataca 3900 actttgcatg tgcaaatgac tgccgagcca gatggccact tccagcaaat ggcaaccaac 3960 ctgggttttg ataatggcca gccgtttgta ctcggtcgcc gtgtgcacca cacatcttac 4020 gtagatggta cgcacgatga gagcgcagaa aacggcgtat ttgatggtat gccaggtaaa 4080 gcaggcccgc actatgtgaa tgaccgctgc tcggattgtc acgagcgcaa tggtcgtgca 4140 ccggttgtgg gtattggtga accgttagac cgttgggtgt ttaaagtggg cgacgcaaat 4200 ggtaacccgc atccagatat ggggcgcgta ttgcagccag aagcgaacaa cggtgccgct 4260 agcgagggca cgccaaccat cgcattcttt agcgaagaaa acggtttgcg caaaccaaac 4320 tatgcattta gcggcataac gccagacact ttctctgcgc gtattgcccc acagctaaat 4380 ggcattggtt tgctcgaagc gattccagaa agtgcaattt tagcgcaaga agatgtgaac 4440 gatgcgaacg gtgatggtat ctccggtaaa gcgcagcgct ctatcgaccc tgtaaccggt 4500 gaaacccgct taggtcgctt tggctacaaa gcagcaacca gcagtgtaaa gcatcaagtg 4560 gctgcggcgt ttaatacgga tataggtgta cgtacctctg taatgcccaa cccagattgc 4620 ggctcgaatc aaaacgattg tggcccaagt ggtgcggaac tagcagatga gcatttagat 4680 aaccttgtta agtatgtttc tttactgggt gttcgcccac agcgagacta caacgatgcg 4740 caagtactac agggtaagca agtgtttaac gatgctggct gtgtgagttg tcataccgac 4800 acttaccaaa catcgcaata tcacccgctt gcagagttac gcagccaaac tattcacccc 4860 tacacagatc tcttgctgca cgatatgggc ccaggtttgg ccgataactt aggtgaaggc 4920 gatgcaacgg gtgccgaatg gcgtaccgcg ccgctttggg gcttaggttt gtctgcttgt 4980 gttacgggtg gtgtagaagg tggtcgcggc tgggatgatt ttggcttaga tggctacgaa 5040 acctgtactc cacatcacag ctacttacac gatggtcgcg cacgcaccat tgaagaagct 5100 attttgtggc acggtggcga aggtgaaaac tcgaagcaag cgtaccaaaa cttaagcaat 5160 agcgagcgcg atgctctact cgcattctta aattccctct aa 5202 <210> 159 <211> 1441 <212> PRT <213> Microbulbifer degradans <400> 159 Met Thr Thr Arg Asn Thr Phe Lys Leu Ser Lys Leu Leu Leu Ser Leu   1 5 10 15 Gly Val Leu Thr Ala Ser Ser Gln Leu Val Ala Gln Asp Leu Ile Trp              20 25 30 Ser Asp Glu Phe Glu Gly Asp Lys Ile Asp Arg Ser Ile Trp Ser Tyr          35 40 45 Asn Val Gly Gly Ser Gly Asn Gly Asn Gly Glu Leu Gln Tyr Tyr Thr      50 55 60 Ala Asn Ala Thr Asn Ser Arg Ile Glu Asp Gly Asn Leu Val Ile Glu  65 70 75 80 Ala Arg Arg Glu Glu Met Glu Gly Lys Gln Phe Thr Ser Ala Arg Leu                  85 90 95 His Thr Asn Gly Arg Phe Ser Phe Lys Tyr Gly Thr Leu Glu Ala Arg             100 105 110 Ile Lys Leu Pro Lys Leu Asp Asp Gly Leu Trp Pro Ala Phe Trp Thr         115 120 125 Leu Gly Asp Asn Phe Gly Val Asp Gly Trp Pro Lys Ser Gly Glu Ile     130 135 140 Asp Ile Leu Glu Ala Gly Tyr Lys Ala Ala Arg Glu Ala Gly Thr Thr 145 150 155 160 Asn Thr Ala Val Ser Gly Ala Val His Trp Trp His Glu Ser Gly Asp                 165 170 175 Trp Ser Asp Trp Leu Gln Ala Asp Ala His Ala Glu Thr His Val Thr             180 185 190 Thr Pro Met Asn Glu Ala Tyr His Thr Tyr Arg Leu Glu Trp Thr Pro         195 200 205 Ser Glu Leu Ser Ile Ser Val Asn Asp Asn Thr Tyr Phe Thr Met Asp     210 215 220 Ile Thr Asp Pro Asn Met Ser Glu Phe His Gly Ala Gln His Leu Leu 225 230 235 240 Leu Asn Leu Ala Val Gly Gly Trp Asn Phe Val Glu Ile Glu Asp Pro                 245 250 255 Ala Leu Ile Thr Ala Asp Phe Pro Ala Gln Met Leu Val Asp Tyr Val             260 265 270 Arg Leu Tyr Ser Asn Glu Phe Thr Glu Val Phe Asp Ala Asn Glu Asn         275 280 285 Leu Pro Thr Gly Asp Phe Gly Val Met Thr Asp Leu Thr Pro Val Phe     290 295 300 Asn Glu Leu Asn Trp Gly Asp Arg Met His Leu Tyr Ile Trp Asn Asn 305 310 315 320 Met Glu Val Ala Gly Ile Asp Pro Tyr Glu Gly Thr Ser Val Leu Ala                 325 330 335 Tyr Asp Val Ala Pro Asp Ala Trp Trp Gly Met Gly Leu Leu His Lys             340 345 350 Asp Tyr Asn Met Arg Asn Tyr Lys His Gly Tyr Leu His Phe Met Met         355 360 365 Lys Thr Thr Ala Thr Ser Asp Ile Ser Ile Asn Met Ala Ser Thr Ser     370 375 380 Gly Gly Glu Gly Ala Val Val Leu Ala Asn Gly Gly Glu Glu Tyr Gly 385 390 395 400 Leu Glu Arg Asp Gly Glu Trp His Glu Val Asn Ile Pro Leu Ala Lys                 405 410 415 Phe Gly Ala Met Asp Phe Glu Thr Ile Lys Thr Phe Phe Ser Met Ser             420 425 430 Gly Pro Gly Gln Ala Glu Ala Phe Gln Ile Ala Val Asp Asp Ile Tyr         435 440 445 Leu Lys Ser Ser Ile Ala Leu Pro Arg Pro Glu Phe Gly Ser Phe Gly     450 455 460 Ile Tyr Thr Glu Ser Pro Glu Asn Met Ser Ala Gly Asn Phe Gly Phe 465 470 475 480 Gly Val Glu Gly Asp Leu Phe Leu Trp Ala Asp Thr Leu Glu Leu Leu                 485 490 495 Pro Gly Glu Val Val Glu Gly Asn Ala Ser Leu His Leu Lys Ser Thr             500 505 510 Gly Gln Gly Trp Phe Gly Met Gly Leu Thr Ala Arg Glu Gly Phe Asn         515 520 525 Leu Ser Ala Phe Asp Asn Ala Asp Ala Lys Leu His Leu Ser Met Lys     530 535 540 Thr Thr Asp Gln Thr Asp Phe Gln Val Gly Ile Lys Ser Gly Ser Val 545 550 555 560 Asn Asp Ile Gly Gln Val Trp Ile Lys Phe Thr Pro Gly Asn Asp Pro                 565 570 575 Tyr Gly Phe Ala Arg Asp Gly Gln Trp His Asp Leu Val Ile Pro Met             580 585 590 Ser Asp Ile Ala Gln Asp Leu Asp Met Phe Asp Val Arg Gln Val Phe         595 600 605 Gln Leu Leu Gly Phe Gly Glu Ile Glu Ser Leu Ala Ile Asp Asn Ile     610 615 620 Tyr Ile Ser Gly Gly Gly Ala Ser Thr Pro Glu Ile Val Asn Pro Ser 625 630 635 640 Glu Pro Val Asn Arg Ala Pro Met Ala Ala Ile Lys Pro Ser Val Asn                 645 650 655 Gly Gly Pro Ala Thr Leu Ser Val Asp Phe Asp Ala Ser Gln Ser Gly             660 665 670 Asp Val Asn Gly Asp Ala Leu Thr Tyr Thr Trp Asp Phe Gly Asp Gly         675 680 685 Thr Thr Ala Thr Gly Val Gln Val Ser His Asp Phe Glu Lys Glu Gly     690 695 700 Asn Tyr Arg Val Ser Leu Ile Val Ser Asp Gly Gln Ala Thr Asp Glu 705 710 715 720 Thr Ala Ala Ile Ile Thr Val Asp Asp Asn Tyr Gly Leu Ser Arg Ser                 725 730 735 Asp Lys Arg Gly Leu Gly Phe Gly His His Ser Val Ala Asp Phe Glu             740 745 750 Ala Ile Ser Gln Gly Ile Ser Trp Trp Tyr Asn Trp Ser Ile Lys Pro         755 760 765 Asp Ser Leu Ile Gln Asp Val Tyr Gln Asn Tyr Gly Val Glu Phe Val     770 775 780 Pro Met Ala Trp Asn Gly Gly Phe Asp Asp Gln Ala Met Arg Asp Tyr 785 790 795 800 Ile Asn Ala His Arg Asp Asp Val Lys Tyr Ile Leu Ala Phe Asn Glu                 805 810 815 Pro Asn Phe Leu Glu Gln Ala Asn Met Thr Pro Ser Gln Ala Ala Ala             820 825 830 Glu Trp Pro Arg Leu Glu Ala Ile Ala Asp Glu Phe Gly Leu Lys Ile         835 840 845 Val Ser Val Ala Met Asn Phe Cys Gly Asn Cys Val Thr Glu Asn Gly     850 855 860 Thr Thr Tyr Tyr Asp Pro Ile Asp Tyr Phe Asp Asp Phe Phe Glu Val 865 870 875 880 Cys Pro Asp Cys Arg Val Asp Ala Leu Ser Ile His Ala Tyr Met Gly                 885 890 895 Gly Val Gly Gly Ile Glu Trp Tyr Ile Asp Leu Phe Ala Lys Tyr Asn             900 905 910 Leu Pro Ile Trp Met Thr Glu Phe Ser Ala Trp Asp Asp Thr Thr Thr         915 920 925 Glu Glu Glu Gln Ile Phe Phe Met Thr Gln Ala Val Asp Tyr Met Glu     930 935 940 Gln His Glu Asn Val Glu Arg Tyr Ala Trp Phe Thr Gly Arg Arg Asn 945 950 955 960 Gly His Pro Tyr Asn Gly Leu Phe Asp Tyr Arg Gln Ser Gly Val Leu                 965 970 975 Thr Glu Leu Gly Ser Ala Tyr Ile Asn Met Pro Val His Gly Glu Asp             980 985 990 Ser Ile His Thr Leu Pro Lys His Ile Glu Ala Glu Ala Tyr Ala Tyr         995 1000 1005 Gln Ser Gly Gly Arg Val Ala Pro Thr Thr Asp Ala Asn Gly Phe Leu    1010 1015 1020 Gln Met Gly Glu Asn Ala Ala Gly Ser Trp Val Glu Tyr Asn Val Ile 1025 1030 1035 1040 Asn Pro Ala Thr Arg Ser Tyr Asn Val Ala Val Arg Val Asn Ser Glu                1045 1050 1055 Thr Gly Gly Thr Ile Thr Val Leu Val Asp Gly Val Glu Lys Gly Gln            1060 1065 1070 Ile Pro Val Pro Ala Ala Ala Asp Ala Gln Ala Trp Ala Thr Val Asp        1075 1080 1085 Thr Asp Leu Glu Ile Thr Ala Gly Glu His Asp Val Arg Leu Val Phe    1090 1095 1100 Gly Ala Ser Val Asn Leu Asn Trp Leu Asn Ile Gly Asp Ala Leu Ser 1105 1110 1115 1120 Pro Glu Leu Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu                1125 1130 1135 Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu            1140 1145 1150 Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu        1155 1160 1165 Pro Glu Pro Glu Pro Glu Pro Glu Val Asp Pro Thr Ala Pro Leu Ala    1170 1175 1180 Cys Thr Ala Thr Ala Ser Ser Gln Glu Gly Ala Leu Ala Pro Ser Phe 1185 1190 1195 1200 Val Cys Asp Gly Asp Ala Gly Thr Arg Trp Ser Ser Glu Trp Asn Asp                1205 1210 1215 Asn Glu Thr Ile Thr Leu Asp Leu Gly Asp Val Tyr Glu Ile Ser Ser            1220 1225 1230 Ile Asn Leu Thr Trp Glu Asn Ala Tyr Gly Ser Lys Tyr Asp Ile Leu        1235 1240 1245 Val Ser Asp Asp Asn Val His Trp Thr Leu Ala Tyr Ser Glu Gln Ala    1250 1255 1260 Gly Asp Gly Gly Ile Asp Asn Leu Ala Val Thr Ala Thr Gly Gln Tyr 1265 1270 1275 1280 Val Arg Leu Leu Gly Leu Glu Arg Gly Ile Gly Tyr Gly Phe Ser Leu                1285 1290 1295 Phe Ser Phe Asp Val Tyr Gly Ala Leu Lys Pro Val Val Val Val Pro            1300 1305 1310 Glu Gly Asp Leu Ala Leu Gly Lys Pro Thr Ser Ala Ser Ser Ser Glu        1315 1320 1325 Trp Ala Glu Pro Thr Ala Ala Asn Ala Thr Asp Gly Asn Thr Gly Thr    1330 1335 1340 Arg Trp Ala Ser Ala Trp Thr Asp Ser Glu Trp Ile Ser Val Asp Met 1345 1350 1355 1360 Gly Glu Val Tyr Pro Val Gly Arg Val Val Leu Asn Trp Glu Gly Ala                1365 1370 1375 Tyr Gly Lys Gly Tyr Asn Ile Gln Thr Ser Asn Asn Gly Ala Asp Trp            1380 1385 1390 Thr Thr Val Tyr Thr Glu Thr Ala Gly Asp Gly Gly Thr Asp Glu Ile        1395 1400 1405 Ile Leu Pro Phe Ala Ser Gly Arg Tyr Ile Arg Val Leu Gly Thr Glu    1410 1415 1420 Arg Gly Thr Gly Tyr Gly Tyr Ser Leu Trp Asp Phe Glu Ile Tyr Ala 1425 1430 1435 1440 Asp     <210> 160 <211> 4326 <212> DNA <213> Microbulbifer degradans <400> 160 atgacaacgc gaaatacttt caagctgagt aagctcctgc tttcgctagg tgtacttacc 60 gcttcttcgc aactcgtcgc ccaagaccta atttggtccg acgaattcga aggcgataaa 120 attgaccgca gcatatggtc ttacaatgtg ggtggtagtg gtaacggcaa tggtgagttg 180 cagtactaca ccgccaatgc caccaattca cgcattgaag acggcaacct cgttattgag 240 gcgcgccgcg aagagatgga aggtaagcag tttacttctg cacgtctcca tacaaatggt 300 cgcttttctt ttaaatacgg taccttagag gcaagaatta aattgcctaa gttagacgat 360 ggcctgtggc cagcgttttg gactttgggt gataactttg gtgtagacgg ctggccaaaa 420 tctggtgaga tcgatatttt agaagccggc tacaaggctg cgcgcgaagc gggcaccacc 480 aatactgcgg tgtctggtgc tgtgcactgg tggcacgaaa gtggcgattg gagtgattgg 540 ttgcaggccg atgctcatgc cgaaacgcat gttacaacgc ctatgaacga ggcgtatcac 600 acctatcgtt tagagtggac tcctagcgag ctatctattt ctgtaaacga caatacctat 660 ttcaccatgg atattaccga cccaaacatg tcggaatttc acggtgctca gcatttgctg 720 cttaacttgg ctgtaggtgg ttggaacttt gtagaaattg aagacccagc gcttattact 780 gcagatttcc ctgcacaaat gcttgtggac tacgtgcgct tatacagcaa cgaattcacc 840 gaagtgtttg acgcaaacga aaacttacca accggtgact tcggcgtaat gaccgatttg 900 acccccgtgt tcaatgaatt gaactggggt gatcgtatgc acctgtacat ttggaacaat 960 atggaagtag cgggcataga cccgtacgaa ggcacttctg tattggctta tgatgtggcg 1020 ccagatgcgt ggtggggcat gggtcttttg cataaagact acaacatgcg caactacaaa 1080 cacggctacc tgcacttcat gatgaagaca accgctacct ccgatatcag cattaacatg 1140 gccagtacct ctggcggtga aggtgctgtt gtattggcaa acggcggtga agagtacggt 1200 ttagagcgcg acggcgaatg gcatgaggtg aatattcctc tggctaaatt cggcgcgatg 1260 gatttcgaaa ccatcaaaac tttcttcagc atgtctggcc caggccaagc agaagcattc 1320 caaattgctg tggacgacat ttatctaaaa agcagtattg ctttacctcg cccagagttt 1380 ggcagcttcg gtatttacac cgaaagccca gaaaatatga gcgcaggtaa ctttggtttt 1440 ggtgtagaag gtgatttatt tctttgggct gataccttag agctattgcc aggcgaagtg 1500 gttgaaggca atgcatcact gcatttgaaa tcaaccggtc aaggttggtt cggtatgggc 1560 ttaacggctc gcgaaggctt taacctttct gcgtttgata acgccgatgc gaaattacac 1620 ctctcaatga aaaccacaga tcaaactgat ttccaagtgg gcattaagag cggcagcgta 1680 aacgacatcg gtcaagtgtg gattaagttt acccctggta acgacccata cggtttcgca 1740 cgcgacggtc agtggcacga cttagttata ccgatgtctg atatcgcaca agatttagat 1800 atgttcgatg tgcgtcaagt atttcaattg ttaggttttg gcgaaataga aagcctcgca 1860 attgacaata tttatattag cggtggtggt gcatctacac cggaaattgt taacccaagt 1920 gaacctgtaa accgcgcacc gatggctgca attaagcctt ctgtaaatgg cggccctgca 1980 acattgagtg ttgatttcga cgcgagccaa tctggcgacg taaacggcga tgcgcttacc 2040 tatacctggg actttggtga cggtactact gcaaccggcg tacaagtaag tcacgacttt 2100 gaaaaagaag gtaactaccg cgttagctta attgtaagcg acggccaagc gacagacgaa 2160 actgcagcca ttattactgt tgacgataac tacggtttat cgcgcagcga taagcgcgga 2220 cttggttttg gtcaccactc agttgccgac ttcgaagcta tttcgcaggg tatttcttgg 2280 tggtacaact ggtctattaa gccagattca ctgattcaag atgtttacca aaactacggc 2340 gtagagtttg tacctatggc gtggaacggt ggttttgacg atcaagcaat gcgcgattac 2400 atcaatgcgc accgcgacga cgttaaatac attcttgcgt tcaacgagcc taacttctta 2460 gagcaagcca acatgacgcc ttcgcaggcg gcagccgagt ggccacgttt agaagccata 2520 gccgatgagt ttggtttgaa aattgtttcc gttgctatga acttctgtgg caactgtgta 2580 acagaaaatg gcactaccta ttacgacccc attgattact ttgacgattt cttcgaagtg 2640 tgccctgact gccgtgtaga tgcgctttct atccatgctt acatgggtgg tgttggcggc 2700 atcgagtggt atatcgatct gtttgcaaaa tacaaccttc caatttggat gaccgagttt 2760 tctgcatggg acgataccac cacagaagaa gagcaaatct tctttatgac ccaagcagta 2820 gactacatgg agcaacacga aaatgtagaa cgctatgcgt ggttcactgg tcgtcgcaat 2880 ggtcacccgt acaacggttt gtttgattac cgccagtcgg gtgtacttac cgagctaggc 2940 agcgcgtaca tcaacatgcc tgtgcacggt gaagacagca ttcacacttt accaaagcat 3000 attgaagcag aagcttacgc ttaccaaagc ggtggacgtg tagcacctac tactgatgca 3060 aatggcttct tgcaaatggg cgagaacgct gctggctctt gggttgaata caacgtaata 3120 aacccagcga ctcgcagtta caacgtagcg gtacgtgtaa acagcgaaac cggcggcacc 3180 attaccgtat tggtagacgg tgttgagaaa ggccaaattc ctgtacctgc agcagctgat 3240 gcgcaagctt gggcaactgt ggataccgat ttggaaatta ctgcaggtga gcacgatgtg 3300 cgcttggtat ttggtgcaag tgttaacctt aactggttaa acattggcga tgcgttaagc 3360 ccagaactag aaccagaacc agaaccagaa ccagaacctg aaccagagcc tgaaccagaa 3420 cctgaaccag agccagaacc agaaccagaa ccagaaccag aaccagagcc tgagccagag 3480 ccagagccag agccagagcc agagcctgag cctgagccag agccagaagt agacccaact 3540 gcaccattag cgtgtaccgc tacagcctca tcgcaagaag gcgcgttagc accaagcttt 3600 gtgtgtgatg gtgatgccgg cacccgttgg tcatccgagt ggaacgataa cgaaaccatt 3660 acgttagacc taggtgatgt ttatgaaata tctagcatta acctaacgtg ggaaaatgcc 3720 tacggaagca agtacgacat attggtttcg gacgataacg tacactggac gcttgcctac 3780 agcgaacaag ctggcgatgg cggtatagat aacttagcag taacggctac tggccaatac 3840 gtgcgcctgc ttggtttaga gcgcggcatt ggttacggct tctctctgtt tagtttcgat 3900 gtttacggtg cactaaaacc tgtagtggtt gtaccagaag gtgatttggc gttaggtaag 3960 cctacgagtg cttcttcatc ggaatgggca gagccaaccg ccgctaatgc aaccgatggc 4020 aacaccggca cgcgttgggc cagtgcttgg acagacagtg agtggatttc tgtagatatg 4080 ggtgaagtgt atcctgttgg tcgagtggta cttaactggg aaggtgcata cggtaaaggc 4140 tataacattc aaacctcaaa caatggggct gattggacta ccgtttacac cgaaaccgca 4200 ggcgatggcg gtacagatga aattattctg ccattcgcgt ctggccgcta tattcgtgta 4260 ctaggtaccg agcgtggcac aggttacggt tactcactgt gggattttga aatctacgca 4320 gattaa 4326 <210> 161 <211> 1207 <212> PRT <213> Microbulbifer degradans <400> 161 Met Gln Pro Ser Ala Tyr Gly Leu Tyr Cys Ala Ala Cys Tyr Ser Gln   1 5 10 15 Lys Ser Lys Cys Glu Phe Ile Met Ile Lys Asn Asn Leu Ile Lys Tyr              20 25 30 Ala Val Arg Cys Ala Ala Leu Gly Ala Leu Ser Thr Ser Ala Val His          35 40 45 Val Ser Ala Thr Asp Tyr Asn Asp Pro Tyr Trp Thr Asn Pro Glu Val      50 55 60 Asn Ser Asp Phe Val Asp Asn Phe Asn Gln Thr Gln Ile Asp Arg Ser  65 70 75 80 Lys Trp Leu Val Glu Thr Asn Ile Phe Val Asn Gly Glu Asp Ile Asp                  85 90 95 Tyr Gln Asp Val Glu Tyr Pro Gln Ala Asp Trp Thr Ile Gly Val Gly             100 105 110 Gln Asp Asp Pro Ala Ala Ile Asp Gly Lys Ala Leu Ile Leu Lys Ala         115 120 125 Arg Tyr Met Asp Gly Glu Val Gln Asp Tyr Tyr Gly Gly Asp Pro Ala     130 135 140 Asn Pro Gly Lys Pro Leu Phe Ile Arg Ser Gly Arg Ile Glu Ser Gln 145 150 155 160 Ile Thr Asp Asp Thr Thr Phe Thr Tyr Gly Lys Phe Glu Ala Arg Leu                 165 170 175 Lys Met Pro Pro Ala Arg Asn Gly Glu Phe Pro Ala Trp Trp Leu Leu             180 185 190 Gly Asn Phe Pro Asp Val Gly Trp Thr Ala Cys Gln Glu Leu Asp Ile         195 200 205 Met Glu Phe Thr Gly Asn Asn Gly Met Asn Ile Pro Gln Thr Tyr Trp     210 215 220 Thr Ala Pro Tyr Ala Val His Gly Gly Thr Thr Val Gly Leu Ala Gly 225 230 235 240 Leu Gly Ile Thr Asn Pro Gln Glu Gln Tyr Val Thr Tyr Gly Ile Ile                 245 250 255 Lys Thr Pro Ser Lys Val Glu Trp Tyr Ile Asn Gly Val Leu Thr Asn             260 265 270 Thr Phe Ser Arg Asp Asn Gln Gly Asp Asp Gln Pro Trp Pro Tyr Val         275 280 285 Thr Pro Met Arg Met Ile Leu Asn His Ala Ile Thr His Val Glu Trp     290 295 300 Pro Asp Val Gly Asn Tyr Asn Ala Tyr Ser Pro Asp Ala Asn Arg Pro 305 310 315 320 Ser Ala Thr Gly Trp Ser Tyr Val Asp Asn Asn Gly Val Thr Gln Tyr                 325 330 335 Glu Tyr Ile Asp Val Asp Ala Met Asn Ala Asn Ile Gly Arg Ala Gly             340 345 350 Thr Asp Phe Val Val Asp Tyr Val Ala His Trp Pro Leu Pro Thr Ser         355 360 365 Asp Ala Glu His Lys Tyr Val Asp Asp Ser Lys Ser Ser Phe Phe Arg     370 375 380 Glu Ser Gly Asn Thr Lys Gly Phe Tyr Asn Leu Lys Gly Trp Leu Ala 385 390 395 400 Pro Val Ser Val Thr Ala Asp Gly Phe Asp Gln Pro Gly Trp Asp Thr                 405 410 415 Asp Val Arg Asp Asn Gly Ala Asp Asn Ala Ala Asp Gly Tyr Val Gly             420 425 430 Ser Lys Trp Ala Thr Pro Asn Asp Asp Gly Ile His Trp Val Glu Val         435 440 445 Asp Tyr Gly Gln Asp Lys Gln Ile Asn Tyr Leu Trp Leu Glu Trp Ala     450 455 460 Trp Asn Leu Pro Ala Asp Tyr Asp Ile Tyr Gly Lys Ser Ser Thr Gly 465 470 475 480 Ala Trp Glu Phe Ile Thr Ser Ser Gln Gln Glu Val Ala Thr Trp Ala                 485 490 495 Thr His Val Phe Asp Val Asn Arg Thr Tyr Arg Tyr Leu Lys Leu Val             500 505 510 Thr Lys Gly Arg Ile Asp Lys Ser Ser Pro Ile Trp Leu Leu Glu Leu         515 520 525 Met Ala Phe Glu Asp Val Pro Asn Met Tyr Pro Lys Pro Ala Gly Thr     530 535 540 Thr Met Pro Asn Arg Ser Val Asn Val Leu Asn Asn Gly Asn Phe Ser 545 550 555 560 Gln Gly Leu Asp Ser Trp Gly Thr Glu Ala Phe Asp Gly Ala Asn Pro                 565 570 575 Thr Tyr Asn Val Gln Asn Gly Ala Ala Val Ile Ala Leu Thr Asn Asp             580 585 590 Gly Gly Leu Ser Gly Ser Val Gln Leu His Thr Ser Gly Phe Gly Leu         595 600 605 Lys Arg Asp Tyr Arg Tyr His Ile Ser Phe Asp Ala Arg Ala Asp Val     610 615 620 Ala Arg Asn Leu Met Val Arg Leu Ala Glu Asn Asn Leu Asn Pro Ser 625 630 635 640 Ala Ala Gly Thr Tyr His Val Glu Thr Val Ala Val Gly Thr Ser Phe                 645 650 655 Asn Thr Tyr Ser Phe Thr Tyr Asp Tyr Thr Gly Gln Gly Glu Pro Ala             660 665 670 Arg Leu Ala Phe Leu Leu Gly Gly Met Gly Thr Ala Thr Thr Tyr Ile         675 680 685 Asp Asn Val Val Ile Arg Glu Gly Asp Phe Ile Gly Ser Gly Glu Pro     690 695 700 Leu Val Ala Ala Ile Ser Ser Thr Asn Phe Val Ser Ala Ser Asn Gly 705 710 715 720 Trp Glu Thr Glu Trp Trp Gly Ala Pro Ala Arg Ala Val Asp Gly Asn                 725 730 735 Leu Gly Asn Lys Ala Ser Gly Asn Asp Gly Glu Ala Glu Gly Met Asp             740 745 750 Leu Asp Ile Thr Val Arg Ile Asp Glu His Tyr Asp Val Arg Ala Val         755 760 765 Leu Val Ala Gly Asp Asn Ser Pro Glu Arg Ser Leu Asp Gln Phe Arg     770 775 780 Val Glu Phe Gly Asn Gly Thr Pro Leu Met Gly Trp Thr Asp Ser Thr 785 790 795 800 Thr Glu Gly Val Tyr Glu Glu Phe Ala Asn Phe Asn Asn Thr Pro Ala                 805 810 815 Ala Gly Glu Arg Asp Phe Lys Phe Phe Phe Arg Pro Pro Ala Gly Gln             820 825 830 Leu Val Glu Val Ala Asp Val Gln Leu Leu Ala Val Asp Leu Met Pro         835 840 845 His Arg Ile Ser Ala Ile Ala Leu Asp Glu Gly Gly Thr Ile Ser Pro     850 855 860 Ala Gly Thr Thr Arg Tyr Ser Arg Asn Asn Thr Asp Asp Ala Thr Tyr 865 870 875 880 Thr Phe Thr Ala Pro Gln Gly Gln Ser Val Ser Asn Val Ile Val Asp                 885 890 895 Gly Val Ser Met Gly Pro Leu Asn Ser Tyr Thr Phe Thr Asp Ile Asn             900 905 910 Ala Asp His Thr Leu Ala Val Ser Phe Gly Gly Glu Val Glu Gln Pro         915 920 925 Glu Thr Gly Asp Ala Ile Asn Tyr Ala Pro Glu Ala Tyr Ala Thr Ala     930 935 940 Ser Ser Glu Leu Gln Thr Ala Ala Leu Ala Asn Asp Gly Asp Ala Gly 945 950 955 960 Ser Arg Trp Glu Ser Glu His Gly Ala Gly Pro Ser Trp Leu Ala Leu                 965 970 975 Glu Leu Asn Asp Thr Val Arg Val Ser Lys Val Val Ile Asp Trp Glu             980 985 990 Ala Ala Asn Ala Gly Thr Tyr Glu Ile Gln Gly Ser Leu Asn Gly Ile         995 1000 1005 Asp Trp Asn Thr Leu Glu Val Val Ser Gly Gly Ala Phe Gly Asn Arg    1010 1015 1020 Thr Asp Thr Val Leu Leu Asp Gly Ser Ala Ser Asn Ser Val Asn His 1025 1030 1035 1040 Ile Arg Ile Tyr Gly Val Glu Arg Ser Ala Gly Asn Asn Trp Gly Tyr                1045 1050 1055 Ser Ile Phe Asn Val Glu Val Trp Gly Glu Ala Gly Asp Thr Ser Met            1060 1065 1070 Glu Glu Glu Val Glu Ile Glu Pro Val Ser Ala Ala Ala Ser Ser Asp        1075 1080 1085 Phe Gln Ala Ala Ala Asn Ala Ile Asp Ala Asp Ala Gly Ser Arg Trp    1090 1095 1100 Glu Ser Ala His Ala Asp Ala Thr Ala His Leu Thr Leu Asp Leu Ala 1105 1110 1115 1120 Ser Thr Tyr Lys Leu Val Ser Val Ala Ile Asp Trp Glu Ala Ala Asn                1125 1130 1135 Ala Gly Ala Tyr Thr Leu Glu Gly Ser Ser Asp Gly Val Asn Trp Thr            1140 1145 1150 Thr Ile Ala Ser Phe Thr Gly Gly Ala Phe Gly Asn Arg Thr Asp Thr        1155 1160 1165 Leu Thr Val Ser Gly Ser His Arg Phe Val Arg Ile Asn Cys Thr Glu    1170 1175 1180 Lys Ser Ala Gly Asn Asn Trp Gly Tyr Ser Ile Tyr Asp Val Arg Leu 1185 1190 1195 1200 Thr Ala Leu Leu Pro Thr Pro                1205 <210> 162 <211> 3624 <212> DNA <213> Microbulbifer degradans <400> 162 atgcaaccgt ctgcgtacgg tttgtattgt gctgcgtgtt attcacaaaa atcaaagtgt 60 gagtttatca tgattaaaaa taacctaatt aaatatgccg ttagatgtgc agccttaggc 120 gcactctcta caagtgcagt acatgttagt gctacagatt acaacgaccc atattggaca 180 aaccccgaag tgaattctga ctttgtggat aatttcaacc aaacccaaat tgaccgaagt 240 aagtggttgg ttgaaacaaa tatttttgta aatggcgaag atatcgatta tcaagatgtg 300 gagtacccac aagccgattg gacaattggc gtagggcagg acgaccccgc cgcaatagac 360 ggcaaggcgc ttatattaaa agcgcgctac atggatggag aagtgcaaga ttactacggc 420 ggtgacccgg caaacccagg caagccattg tttattcgtt cggggcgtat tgagtcgcaa 480 ataaccgacg atacaacttt tacctatggg aaattcgaag cccgattaaa aatgccgcct 540 gcacgcaacg gcgaattccc cgcgtggtgg ctattaggta actttcctga tgtgggttgg 600 accgcttgcc aagagttaga cattatggaa tttaccggca acaacggtat gaatattcca 660 caaacctatt ggaccgcccc ctatgctgta cacggtggta caacagtcgg tttagcaggc 720 cttggtatta ctaatccgca agagcagtat gttacttacg gtattattaa aacgccgtca 780 aaagtagagt ggtatatcaa cggtgtgctc accaatactt tttctcgcga caaccaaggt 840 gacgatcaac cttggccgta cgtcaccccc atgcgcatga ttttaaacca cgcaattact 900 catgtggaat ggccggatgt aggtaactac aacgcttatt ccccagatgc caacaggccc 960 agcgccacag gttggagcta tgtggataac aatggcgtaa cccaatacga atatattgat 1020 gtagatgcaa tgaacgcaaa tatcggtcgg gcgggtaccg attttgttgt ggattatgtt 1080 gcccattggc cgctacctac tagcgatgcc gaacacaaat atgtagacga tagtaaatcc 1140 agctttttcc gcgaatcggg taacactaaa ggcttttaca atttaaaagg ctggttagcg 1200 ccagtgtcag tgactgctga tggatttgat caaccaggtt gggataccga tgtgcgcgac 1260 aatggcgccg ataatgcagc tgatggctat gtaggctcta agtgggcaac acccaacgac 1320 gacggtattc attgggttga agtggattac ggccaagaca agcaaattaa ttacttgtgg 1380 ttagagtggg cgtggaacct acctgccgat tacgatatat acggtaaaag cagtactggt 1440 gcttgggaat ttatcacctc gtcacaacaa gaagtggcca cgtgggctac gcacgttttt 1500 gatgtaaatc gcacttaccg ttacctaaag ctcgtaacaa aaggccgcat agataaatct 1560 agccctattt ggttgttaga gcttatggca tttgaagatg tgccaaatat gtaccctaaa 1620 cctgcgggta ccacaatgcc taaccgatcc gttaacgtgc taaataatgg caatttttcg 1680 caagggttag atagctgggg aaccgaggcc tttgatggcg caaacccaac ttacaacgtg 1740 caaaatggtg cggcagtcat tgcgcttaca aacgatggtg gcttatcggg ctctgtgcaa 1800 ttgcacactt caggttttgg tttaaagcgt gattatcgct atcacataag tttcgatgct 1860 cgcgccgatg ttgcacgaaa tttaatggtg cgcttagcag aaaacaacct taacccaagc 1920 gcagctggca cctaccatgt agaaaccgtt gctgtgggga caagctttaa cacctatagt 1980 tttacttacg attacacggg gcaaggtgaa cctgctcgac tcgcattttt actgggcgga 2040 atgggtactg cgaccaccta tatcgataat gtggttattc gtgaaggtga ctttattggc 2100 agtggtgaac cgcttgtagc ggctattagc tctaccaact ttgttagcgc cagtaatggc 2160 tgggaaaccg aatggtgggg tgcgcctgct cgtgcggtag atggaaactt aggtaataaa 2220 gcgagcggta acgatggtga agccgaaggc atggatcttg atattaccgt gcgtattgac 2280 gagcattacg acgttagagc ggtattggtt gcgggcgaca attcacctga gcgaagtttg 2340 gatcagttcc gcgttgagtt tggcaatggc acacctttaa tgggctggac cgacagcaca 2400 acagagggtg tatacgaaga gttcgcaaac tttaataaca cgcctgctgc tggcgagcgc 2460 gattttaaat tcttcttccg cccacctgca gggcaactgg tagaagtggc cgatgtgcaa 2520 ttgctagcgg tagatttaat gccccataga atttcagcca tagcgttaga cgagggcggc 2580 actatttcgc cagcaggcac tacgcgctac agcagaaaca acaccgatga tgcaacctac 2640 acgtttaccg ccccacaagg gcaatcggta agtaatgtga ttgtggatgg cgtgtcgatg 2700 ggaccgttaa atagttatac ttttaccgat atcaatgctg atcacacctt agcggtaagc 2760 tttggtggtg aagttgagca accagaaact ggcgatgcta tcaactatgc gcccgaggct 2820 tacgcaactg cgagttccga gttacaaacg gcggcgttag ccaacgacgg cgatgcaggt 2880 tcgcgctggg agagtgagca cggtgcaggc ccttcgtggt tagcgctaga gttaaatgac 2940 acagtgcgcg tatccaaagt ggttattgat tgggaagcgg caaacgctgg cacctatgaa 3000 attcaaggct cgctaaacgg tatagattgg aacaccttag aagtcgttag tggtggagcc 3060 tttggtaatc gtacggatac ggttttacta gatggctctg ctagtaattc tgtcaaccat 3120 attcgtatat acggtgtaga acgcagtgct ggcaacaact gggggtattc aatctttaac 3180 gttgaagtat ggggcgaagc aggtgatacc tcgatggagg aggaagtaga aattgagcct 3240 gtatccgcag cagctagcag tgattttcaa gcggcagcga atgcaataga tgccgatgct 3300 ggtagtcgct gggaaagcgc gcacgcagat gctaccgcac acttaacatt ggatttagca 3360 tcaacctata aactcgtgtc agtcgctata gattgggaag ctgccaatgc tggagcttac 3420 accctagaag gttctagcga tggtgtaaat tggacaacca ttgcaagctt caccggcggt 3480 gctttcggca atagaaccga tactttaacg gtgagtggca gccatagatt tgtacgcatt 3540 aactgtacag aaaaaagtgc aggcaacaat tggggctatt ccatttatga cgtacgtcta 3600 actgcattat tacctacgcc ttaa 3624 <210> 163 <211> 331 <212> PRT <213> Microbulbifer degradans <220> <221> MOD_RES <222> (313) .. (329) Variable amino acid <400> 163 Met Lys Lys Val Phe Ser Leu Ile Ile Gly Leu Ser Leu Ser Leu Pro 1 5 10 15 Val Trp Ala Gly Trp Glu Arg Gln Trp Ile Asp Thr Phe Asp Gly Asp             20 25 30 Ser Val Asp Trp Thr Asn Trp Thr Ala Gln Val Glu Ala Asn Tyr Asn         35 40 45 Asn Glu Val Gln Cys Tyr Thr Ala Asp Glu Thr Ser Ala Asn Lys Asn     50 55 60 Tyr Asp Val Ser Asp Gly Thr Leu Lys Ile Ile Ala Arg Lys Gln Ser 65 70 75 80 Val Glu Cys Ala Gly Leu Gly Gly Gln Asn Lys Thr Trp Thr Ser Gly                 85 90 95 Arg Leu Asn Ser Lys Asp Lys Gln Glu Phe Leu Tyr Gly Arg Ile Glu             100 105 110 Ser Arg Ile Arg Phe His Asn Leu Glu Gly Gly Thr Trp Pro Ala Phe         115 120 125 Trp Met Leu Glu Asn Arg Ile Ser Glu Thr Pro Arg Lys Tyr Asp Asp     130 135 140 Asp Tyr Glu Gln Trp Pro Asn Pro Gly Ala Gly Glu Ile Asp Val Trp 145 150 155 160 Glu Trp Phe Ser Asn Gly Pro Asn Ile Tyr Ile Ile Asn Phe Phe Asn                 165 170 175 Ala Asn Asn Cys Gly Asp Arg His Asp Tyr Val Tyr Pro Asn Gly Gly             180 185 190 Ser Asp Val Leu Asn Trp His Asn Tyr Ala Met Glu Trp Asp Ala Asn         195 200 205 Asn Ile Ser Phe Phe Ile Asp Gly Ser Leu Ile Thr Ser Phe Asp Val     210 215 220 Ser Ser Cys Pro Gln Tyr Lys Glu Lys Met Phe Val Leu Leu Asn Leu 225 230 235 240 Ala Val Gly Gly Asn Leu Gly Gly Thr Ile Asp Pro Asn Leu Ser Leu                 245 250 255 Ala Thr Leu Glu Val Asp Tyr Val Gly Tyr Cys Thr Ala Thr Asn Ala             260 265 270 Asn Asp Tyr Ala Ser Cys Asp Glu Thr Thr Pro Ala Ala Leu Gln Gly         275 280 285 Glu Pro Ser Thr Ser Trp Val Leu Ser Thr Val Asn Ala Thr Ala Thr     290 295 300 Leu Ala Asn Ala Ala Gln Asn Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 305 310 315 320 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Lys Leu                 325 330 <210> 164 <211> 707 <212> PRT <213> Microbulbifer degradans <220> <221> MOD_RES (222) (14) .. (30) Variable amino acid <400> 164 Met Leu Pro Pro Arg Leu Pro Met Gln Arg Lys Ile Ala Xaa Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Asaa Tyr             20 25 30 Asn Asn Glu Val Gln Cys Tyr Thr Ala Asp Glu Thr Ser Ala Asn Lys         35 40 45 Asn Tyr Asp Val Ser Asp Gly Thr Leu Lys Ile Ile Ala Arg Lys Gln     50 55 60 Ser Val Glu Cys Ala Gly Leu Gly Gly Gln Asn Lys Thr Trp Thr Ser 65 70 75 80 Gly Arg Leu Asn Ser Lys Asp Lys Gln Glu Phe Leu Tyr Gly Arg Ile                 85 90 95 Glu Ser Arg Ile Arg Phe His Asn Leu Glu Gly Gly Thr Trp Pro Ala             100 105 110 Phe Trp Met Leu Glu Asn Arg Ile Ser Glu Thr Pro Arg Lys Tyr Asp         115 120 125 Asp Asp Tyr Glu Gln Trp Pro Asn Pro Gly Ala Gly Glu Ile Asp Val     130 135 140 Trp Glu Trp Phe Ser Asn Gly Pro Asn Ile Tyr Ile Ile Asn Phe Phe 145 150 155 160 Asn Ala Asn Asn Cys Gly Asp Arg His Asp Tyr Val Tyr Pro Asn Gly                 165 170 175 Gly Ser Asp Val Leu Asn Trp His Asn Tyr Ala Met Glu Trp Asp Ala             180 185 190 Asn Asn Ile Ser Phe Phe Ile Asp Gly Ser Leu Ile Thr Ser Phe Asp         195 200 205 Val Ser Ser Cys Pro Gln Tyr Lys Glu Lys Met Phe Val Leu Leu Asn     210 215 220 Leu Ala Val Gly Gly Asn Leu Gly Gly Thr Ile Asp Pro Asn Leu Ser 225 230 235 240 Leu Ala Thr Leu Glu Val Asp Tyr Val Gly Tyr Cys Thr Ala Thr Asn                 245 250 255 Ala Asn Asp Tyr Ala Ser Cys Asp Glu Thr Thr Pro Ala Ala Leu Gln             260 265 270 Gly Glu Pro Ser Thr Ser Trp Val Leu Ser Thr Val Asn Ala Thr Ala         275 280 285 Thr Leu Ala Asn Ala Ala Gln Asn Ser Gly Ala Ile Ala Phe Glu Thr     290 295 300 Ser Ala Phe Gly Glu Asn Ala Ser Asp Pro Met Ile Tyr Gln Ala Gly 305 310 315 320 Gln Asn Ile Thr Ala Gly Lys Glu Tyr Thr Leu Ala Phe Asp Val Arg                 325 330 335 Ser Asn Thr Ala Gly Arg Ala Phe Arg Ala Phe Val Ala Ala Ser Ala             340 345 350 Glu Ala Ser Gln Gly Ile Leu Asp Gln Glu Val Val Val Ala Asn Ala         355 360 365 Asp Ala Trp Gln Phe Val Ser Leu Asn Phe Thr Ser Glu Gln Thr Phe     370 375 380 Asn Asp Ala Val Ile Ala Phe Gln Ser Gly Thr Ala Ala Leu Gly Asn 385 390 395 400 Gly Glu Leu Leu Phe Lys Asn Ile Val Leu Thr Glu His Ser Thr Leu                 405 410 415 Tyr Ser Thr Thr Val Val Thr Asn Ala Gly Gly Ser Ile Asn Pro Pro             420 425 430 Gly Pro Thr Val Lys Thr Val Ser Gly Tyr Ser Ser His Phe Thr Ile         435 440 445 Thr Ala Asn Val Thr His Ala Ile Gly Asp Val Phe Ile Asp Gly Val     450 455 460 Ser Val Gly Pro Val Ala Glu Tyr Thr Phe Asp Asn Ile Gln Gly Asp 465 470 475 480 His Glu Ile Glu Val Gln Phe Val Ala Leu Pro Pro Pro Glu Gly Thr                 485 490 495 Glu Ile Leu Thr Pro Val Ala Ala Thr Ala Ser Ser Ser Leu Ser Val             500 505 510 Ala Ser Arg Ala Ile Asp Gly Asp Tyr Ala Thr Arg Trp Glu Ser Arg         515 520 525 His Gly Ile Glu Ala Thr Trp Leu Glu Leu Asp Leu Gly Thr Ala Val     530 535 540 Asp Leu His Ser Ile Leu Ile Asn Trp Glu Ala Ala Asn Ala Lys Ala 545 550 555 560 Tyr Thr Leu Glu Gly Ser Asn Asp Gly Glu Asn Trp Thr Val Leu Ala                 565 570 575 Ser Val Thr Asn Gly Thr Phe Gly Asp Arg Ala Asp Glu Leu Ser Leu             580 585 590 Thr Gly Ser Tyr Ser His Leu Arg Ile Asn Ala Thr Glu Arg Ser Ala         595 600 605 Gly Asn Asp Trp Gly Tyr Ser Ile Trp Asp Ile Val Leu Tyr Ala Tyr     610 615 620 Thr Gln Asp Gln Asn Ala Ser Ser Ser Ser Ser Ser Ser Asn Ser 625 630 635 640 Thr Thr Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser                 645 650 655 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Gly Gly Ser Glu             660 665 670 Pro Ala Ser Ser Gly Ser Gly Arg Met Asp Gly Val Val Phe Leu Phe         675 680 685 Leu Met Val Leu Phe Gly Leu Val Thr Gln Arg Val Val Leu Ala Lys     690 695 700 Leu val ser 705 <210> 165 <211> 2124 <212> DNA <213> Microbulbifer degradans <220> <221> modified_base (222) (40) .. (89) <223> a, c, g, or t <400> 165 atgctaccgc cacgcttgcc aatgcagcgc aaaatagcgn nnnnnnnnnn nnnnnnnnnn 60 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aattataaca acgaagtgca gtgttatacc 120 gcagatgaaa catccgctaa taaaaactac gacgtatctg acggtacgct aaaaataata 180 gcaagaaagc aatccgtcga gtgtgcaggc ctaggtggcc aaaacaaaac atggacatca 240 gggcgattaa acagcaaaga taaacaagag ttcttgtatg gccgtataga atcccgcatt 300 cgcttccaca acctagaggg cggcacgtgg ccggcatttt ggatgctcga aaaccgcata 360 tccgaaaccc cgcgtaaata cgatgatgat tacgagcaat ggcccaaccc cggtgccggt 420 gaaatagatg tgtgggagtg gttttcgaac ggccctaata tttacatcat aaacttcttt 480 aatgctaata actgcggcga taggcacgat tacgtttacc caaatggcgg cagtgatgta 540 ctaaattggc ataactatgc catggagtgg gatgcaaata acattagctt ttttatagat 600 ggcagcctga ttacctcgtt tgatgtgtct tcgtgcccgc agtacaaaga gaaaatgttt 660 gtactactca acttggccgt tggcgggaat ctaggtggta ctatcgaccc caatttatcg 720 ctcgcaacat tagaagtgga ttacgtaggc tactgcacag ctaccaatgc taacgactac 780 gcaagctgtg atgaaacaac acccgctgca ttgcaaggtg agcccagcac atcgtgggtg 840 ttaagcacag taaatgctac cgccacgctt gccaatgcag cgcaaaatag cggagccatt 900 gcgtttgaaa caagcgcttt cggcgaaaat gcatcagacc ctatgattta ccaagcaggg 960 caaaacatca cagccggtaa agaatatacc ttagcgtttg atgtgcgatc caatactgcc 1020 ggccgcgcct ttcgtgcatt tgtggcggct agtgccgaag cctcgcaggg tatattagat 1080 caagaagttg tggttgcaaa tgcagacgca tggcaattcg taagtttaaa cttcacgtca 1140 gaacaaacct ttaacgatgc ggttatagcg tttcaatccg gcactgcagc actgggtaac 1200 ggtgagttgt tgtttaaaaa tatagtgctt accgagcatt ccaccttgta ctcaacaacg 1260 gttgttacca atgctggtgg ttcaataaac cctcctgggc ctacagtaaa aaccgtaagc 1320 ggttacagta gccactttac aattactgca aatgtaaccc atgctattgg cgatgtgttt 1380 atcgatggcg taagcgttgg ccctgttgcc gaatatacgt tcgacaatat acaaggcgac 1440 cacgaaatag aagtgcagtt tgttgcactg ccgccccccg aaggtaccga gatacttacc 1500 cctgtagccg ccactgcgag cagttcactt agtgttgcga gcagagcaat cgatggcgac 1560 tacgcaaccc gctgggaatc gcgccacggt atagaggcta cgtggctaga gttagattta 1620 ggcacagccg tagatttgca ctccatactc ataaactggg aagccgcaaa cgcaaaagcg 1680 tacaccctag aaggctctaa cgatggcgaa aattggacag tactcgcaag cgtaacgaat 1740 ggcacctttg gcgaccgcgc cgatgagcta tcactcactg gcagctactc acacctgcga 1800 ataaacgcaa ccgaaagaag cgcgggtaat gattggggct attccatttg ggatatagtg 1860 ctatacgcat acacgcaaga tcaaaacgca agtagcagct cgtctagttc ttcaaacagt 1920 acaacctcta gttcttctag ctcatccact tcttcgagta gctcttcctc aagctcttct 1980 agttcatctt caagttctag ctctggcggc agtgaacctg cttcgagcgg tagcggcaga 2040 atggatggtg ttgtttttct tttcttgatg gtgttgtttg gtttggttac acaacgcgtc 2100 gtgttagcta aattagttag ttaa 2124 <210> 166 <211> 573 <212> PRT <213> Microbulbifer degradans <400> 166 Met Glu Ala Thr Met Ile Lys Thr Cys Lys Thr Thr Pro Ala Lys Trp 1 5 10 15 Ala Ala Ala Leu Ser Leu Gly Cys Ala Leu Leu Leu Pro Ser Gly Val             20 25 30 Asn Ala Ala Thr Phe Gln Ala Glu Asp Tyr Ser Ala Phe Tyr Asp Thr         35 40 45 Thr Ala Gly Asn Thr Gly Gly Ala Tyr Arg Asn Asp Asn Val Asp Ile     50 55 60 Glu Ala Thr Asn Asp Asn Gly Gly Gly Tyr Asn Val Gly Trp Ile Asp 65 70 75 80 Ala Asn Glu Trp Leu Val Tyr Pro Gly Val Asn Ile Thr Thr Thr Gly                 85 90 95 Asp Tyr Val Ile Asn Val Arg Val Ala Ser Ala Ser Gly Gly Ala Leu             100 105 110 Ser Val Asp Phe Asn Ala Gly Ser Ile Pro Leu Gly Gln Phe Asp Ile         115 120 125 Pro Asn Thr Gly Gly Trp Gln Asn Trp Val Thr Val Ser Lys Thr Val     130 135 140 Thr Leu Thr Ala Gly Thr Tyr Asp Met Gly Val Phe Ala Ser Thr Gly 145 150 155 160 Gly Trp Asn Phe Asn Trp Ile Glu Val Thr Pro Val Asn Asn Gly Gly                 165 170 175 Gly Asn Gly Gly Gly Ser Ala Thr Phe Phe Gln Ala Glu Asp Tyr Ser             180 185 190 Asn Tyr Ser Asp Thr Thr Pro Glu Asn Ile Gly Gly Ala Tyr Arg Asn         195 200 205 Asp Gly Val Asp Ile Glu Thr Thr Ser Asp Ala Asn Gly Gly His Asn     210 215 220 Ile Gly Trp Met Glu Asn Gly Glu Trp Leu Ala Tyr Glu Gly Leu Ser 225 230 235 240 Ile Pro Ser Asn Gly Asn Tyr Ile Ile Lys Ala Arg Val Ala Ser Pro                 245 250 255 Asn Gly Gly Ala Leu Ser Phe Asp Leu Asn Gly Gly Gly Gln Val Leu             260 265 270 Gly Thr Met Asn Ile Pro Ala Thr Gly Gly Trp Gln Asn Trp Gln Thr         275 280 285 Val Glu Leu Ser Thr Asn Ile Asn Ala Gly Thr Tyr Thr Leu Gly Ala     290 295 300 Phe Val Ser Thr Ser Gly Phe Asn Leu Asn Trp Ile Glu Val Val Ala 305 310 315 320 Gly Gly Asp Gly Gly Asn Asn Gly Gly Gly Gly Gly Asn Ile Thr Trp                 325 330 335 Arg Asp Glu Phe Asp Thr Ile Asn Arg Asp Val Trp Asn Phe Glu Thr             340 345 350 Gly Gly Gly Gly Trp Gly Asn Asn Glu Leu Gln Tyr Tyr Thr Asp Gly         355 360 365 Gln Asn Ala Ser Ile Gln Phe Asp Pro Gln Ala Gly Ser Asn Val Leu     370 375 380 Val Ile Glu Ala Arg Lys Glu Thr Gly Gly Gln Cys Trp Trp Gly Gly 385 390 395 400 Asn Cys Gly Tyr Thr Ser Ser Arg Met Asn Thr Arg Phe Lys Lys Ser                 405 410 415 Phe Gln Tyr Gly Arg Ile Glu Ala Arg Met Lys Leu Pro Arg Thr Gln             420 425 430 Gly Ile Trp Pro Ala Phe Trp Met Leu Gly Asp Asn Phe Asn Asn Val         435 440 445 Gly Trp Pro Gln Gly Gly Glu Leu Asp Ile Met Glu His Val Gly Thr     450 455 460 Asn Asn Ile Thr Ser Gly Ala Leu His Gly Pro Gly Tyr Ser Gly Asn 465 470 475 480 Thr Pro Ile Thr Gly His Leu Glu His Gly Ala Ser Ile Asp Ser Gly                 485 490 495 Tyr Arg Val Tyr Ala Val Glu Trp Asp Thr Asn Gly Ile Arg Trp Phe             500 505 510 Val Asp Gly Thr Asn Phe Tyr Ser Val Glu Lys Trp Gln Val Gln Gln         515 520 525 Tyr Gly Glu Trp Val Tyr Asp Gln Pro Phe Trp Ile Leu Leu Asn Leu     530 535 540 Ala Val Gly Gly Asn Trp Pro Gly Asp Pro Asp His Ala Asn Phe Thr 545 550 555 560 Thr Gln Arg Phe Tyr Ile Asp Tyr Val Arg Val Ile Gln                 565 570 <210> 167 <211> 1722 <212> DNA <213> Microbulbifer degradans <400> 167 atggaggcta caatgataaa aacatgcaaa accacaccag ccaagtgggc agcagccctt 60 agcttaggct gcgccctact tttaccctca ggcgtcaatg ctgccacctt ccaagcagaa 120 gattacagcg ccttctacga caccaccgcg ggtaacactg gcggcgccta ccgcaatgat 180 aacgtcgata tagaagccac caatgataac ggcggcggct ataacgtggg ctggatagat 240 gccaacgaat ggctggtata cccaggcgta aatatcacca ccaccggcga ctatgtaatt 300 aatgtacgcg tggcgagcgc aagcggcggt gcactttctg tcgactttaa tgcaggctct 360 attccactgg gtcagttcga tattcccaac actggcggtt ggcaaaactg ggtaaccgtt 420 tcaaaaacag ttactttaac tgccggcacc tacgatatgg gcgtattcgc ctctaccggc 480 ggctggaact ttaactggat agaagtcaca cccgttaata acggtggtgg caatggcggc 540 ggttcagcca cgttctttca agcggaagac tacagcaact actccgacac cacacccgaa 600 aacattggcg gcgcctatcg caatgacggt gtagatatag aaaccacaag cgatgccaac 660 ggcggccaca atataggctg gatggaaaac ggcgaatggc ttgcctacga aggtttatct 720 attcccagta acggcaacta catcattaaa gcgcgtgtag ctagccccaa cggcggcgca 780 ctatcgtttg atttaaacgg cggcggccaa gtgcttggca caatgaacat acccgctact 840 ggcggttggc aaaattggca aaccgtagaa ttgagcacca acattaatgc cggaacctat 900 acgctcggcg cctttgttag tactagtggc tttaacctaa attggataga agtcgttgcc 960 ggtggcgacg gcggcaataa tggtggcggt ggtggcaaca tcacttggcg cgatgaattc 1020 gacacaatca accgcgatgt ttggaatttt gaaaccggtg gcggcggctg gggtaacaac 1080 gaattgcaat actacaccga cggccaaaac gcttccattc agttcgaccc gcaagccggt 1140 agtaatgtgt tagttatcga agcccgcaaa gaaaccggtg gccaatgttg gtggggcggc 1200 aattgcggtt acacctctag ccgcatgaac acgcggttta aaaaatcgtt ccaatacggc 1260 cgtatagaag cacgtatgaa gctgccgcgt acccaaggta tttggccagc gttttggatg 1320 ctaggtgaca actttaataa tgtaggctgg cctcaaggtg gcgaactaga cattatggag 1380 cacgtaggca ccaacaatat cacatcgggt gcattgcacg gcccaggcta cagcggtaat 1440 acgcccatta ccggccattt ggagcacggt gcaagcattg attctggcta ccgtgtttac 1500 gcagtagaat gggataccaa tggtattcgc tggtttgttg acggcaccaa cttctacagc 1560 gtagaaaaat ggcaagtgca acagtacggc gaatgggttt acgaccaacc tttctggata 1620 ctgctaaacc tagcggtggg aggcaactgg cccggcgacc cagaccacgc caactttact 1680 acccaacgtt tttacattga ctatgtgcgg gtgattcagt aa 1722 <210> 168 <211> 742 <212> PRT <213> Microbulbifer degradans <400> 168 Met Lys Lys Leu Lys Leu Leu Glu Leu Ser Leu Val Val Leu Val Ala 1 5 10 15 Leu Gly Leu Ala Ser Cys Gly Gly Ser Asp Asp Lys Lys Thr Pro Asp             20 25 30 Pro Val Glu Glu Pro Val Gly Glu Pro Glu Ser Glu Pro Glu Ser Glu         35 40 45 Pro Glu Ser Glu Pro Glu Ser Glu Pro Glu Pro Glu Asn Ala Trp Gln     50 55 60 Leu Leu Trp Glu Asp Asn Phe Asp Ser Glu Ile Ser Ala Ser Asn Trp 65 70 75 80 Gly Phe Glu Val Asn Cys Thr Gly Gly Gly Asn Asn Glu Lys Gln Cys                 85 90 95 Tyr Thr Asp Arg Ala Asp Asn Ala Tyr Val Asp Glu Ala Gly Ile Leu             100 105 110 His Ile Val Ala Lys Glu Glu Ala Phe Ser Gly Pro Ala Ile Gln Asp         115 120 125 Asp Asp Pro Asn Tyr Asn Pro Asp Asp Thr Ser Ala Ala Arg Asn Tyr     130 135 140 Thr Ser Ala Arg Leu Arg Thr Leu Asp Lys Phe Asp Phe Lys Tyr Gly 145 150 155 160 Arg Val Glu Ile Arg Ala Gln Ile Pro Gly Gly Gln Gly Ser Trp Pro                 165 170 175 Ala Leu Trp Met Leu Pro Ser Asp Lys Val Tyr Gly Gly Trp Pro Ala             180 185 190 Ser Gly Glu Ile Asp Ile Met Glu Ala Val Asn Leu Asp Thr Asp Ala         195 200 205 Ala Asn Ala Val His Gly Thr Leu His Tyr Gly Leu Gln Trp Pro Gln     210 215 220 Trp Ser Thr Ile Gly Ala Ser Tyr Glu Thr Asn Asp Asp Phe Thr Gly 225 230 235 240 Glu Phe His Thr Tyr Ala Ile Glu Trp Glu Ala Asp Gln Ile Arg Trp                 245 250 255 Phe Val Asp Gly Val His Thr Gln Thr Gln Val Ser Asp Asn Trp Tyr             260 265 270 Asn Phe Val Trp Gly Gly Gln Glu Ser Gly Phe Ala Val Ala Asn Pro         275 280 285 Arg Ala Pro Tyr Asp Gln Glu Phe His Leu Ile Met Asn Val Ala Ile     290 295 300 Gly Gly Asn Trp Pro Gly Asp Pro Asp Thr Gly Trp Ala Ser Asp Arg 305 310 315 320 Glu Met Leu Val Asp Tyr Val Arg Val Tyr Gln Cys Asp Ser Glu Ala                 325 330 335 Asp Asp Gly Thr Gly Cys Ala Asn Ala Thr Asp Ala Val Asp Ile Ala             340 345 350 Ile Ser Pro Thr Ala Asp Val Gly Ala Pro Ser Gln Val Glu Tyr Asp         355 360 365 Leu Tyr Ser Asp Gly Leu Gln Thr Pro Ala Phe Thr Asn Ala Gly Val     370 375 380 Thr Leu Ala Ala Asn Val Trp Gln Glu Thr Glu Gly Asn Val Val Thr 385 390 395 400 Thr Thr Gly Asn Leu Gly Asp Asp His Gly Asp Ala Trp Glu Ile Thr                 405 410 415 Phe Asn Gly Thr Gly Asn Val Ser Ile Ala Ile Ala Glu Gln Asp Gly             420 425 430 Val Glu Ser Tyr Val Arg Leu Asn Gly Gly Thr Ala Trp Ser Asn Tyr         435 440 445 Gly Val Leu Ala Phe Asp Met Tyr Val Asp Ser Val Asp Ala Glu Thr     450 455 460 Gly Phe Val Val Lys Met Asp Ser Val Tyr Pro Asn Val Gly Ala Val 465 470 475 480 Asp Ile Ala Thr Pro Ala Ala Gly Glu Trp Thr Arg Val His Val Lys                 485 490 495 Val Ala Asp Ile Leu Ala Asn Pro Ile Ala Gly Gly Gly Gly Leu Asn             500 505 510 Val Ala Gln Ala Val Asn Leu Phe Val Leu Glu Pro Thr Gly Thr Lys         515 520 525 Thr Ala His Val Tyr Val Asp Asn Ile Ser Ile Ser Cys Ala Tyr Asn     530 535 540 Ser Thr Ala Lys Ser Trp Gln Gly Asp Lys Thr Cys Asp Val Ala Ala 545 550 555 560 Val Pro Thr Ala Asp Thr Ser Gly Ile Asp Leu Ser Gly Asn Glu Leu                 565 570 575 Val Ile Phe Asp Glu Gln Glu Pro Asp Phe Trp Gly Phe Gly Gln Phe             580 585 590 Ser Ala Gly Gly Ala Glu Val Ala Met Ser Phe Leu Ala Asp Pro Glu         595 600 605 Asp Ala Ser His Gly Asn Val Leu Gly Phe Ser Tyr Pro Asp Ser Gln     610 615 620 Asn Val Ala Tyr Leu Gln Ser Ala Thr Pro Leu Asn Leu Ser Asp Trp 625 630 635 640 Ala Gly Gly Thr Ile Gln Phe Asp Met Tyr Val Ile Ser Glu Pro Ala                 645 650 655 Asn Val Asn Trp Met Met Lys Val Asp Cys Val His Pro Cys Ser Ser             660 665 670 Gly Asp Ile Pro Leu Thr Thr Asn Ile Asp Gly Val Val Pro Ala Val         675 680 685 Gly Val Trp Gln Thr Tyr Arg Phe Asn Leu Asp Ala Leu Val Ala Gly     690 695 700 Asn Pro Gly Leu Asp Leu Thr Lys Val Asp Thr Pro Leu Val Ile Phe 705 710 715 720 Pro Ala Trp Asp Asn Gln Thr Gly Ala Asn Phe Arg Ile Asp Asn Ile                 725 730 735 Lys Phe Val Arg Ala Asn             740 <210> 169 <211> 2229 <212> DNA <213> Microbulbifer degradans <400> 169 atgaaaaaac taaagcttct agaactttcg ctggtggtgt tggtagccct agggctagcg 60 agctgcggcg gttctgacga caaaaaaacg ccagacccag tagaagaacc cgtgggtgaa 120 ccagaatcag agcctgaatc agagccagaa tctgaacccg aatctgagcc agaaccagaa 180 aacgcatggc aattgctgtg ggaagataat ttcgacagcg aaataagcgc atctaactgg 240 ggcttcgaag ttaactgcac cggcgggggt aacaacgaga aacagtgtta taccgataga 300 gccgacaacg cttatgtaga tgaggcaggc atactgcaca tagttgccaa agaggaagca 360 tttagcggac ccgctattca ggatgatgac cccaactaca accccgatga cacatcggct 420 gcgcgcaatt atacctctgc gcgtttgcgt acattagata agtttgactt caaatatggt 480 cgagtcgaaa ttcgtgcgca aataccaggt gggcagggct catggcctgc attgtggatg 540 ttgccaagtg acaaagtata tggtggttgg ccagccagtg gcgaaatcga cattatggaa 600 gcggttaatt tagatactga tgcggccaat gccgtgcatg gtactttgca ctacggttta 660 cagtggccac aatggtcgac gataggcgct tcttacgaaa caaatgatga tttcaccggt 720 gagttccaca cttatgccat tgagtgggaa gcagaccaaa ttcgctggtt tgtggatggc 780 gtacacactc aaacacaggt gtccgataac tggtacaact ttgtatgggg cgggcaagag 840 tccggctttg ctgtggctaa cccgcgtgca ccttacgatc aagagtttca tttaatcatg 900 aacgtcgcta taggcggcaa ctggccgggt gacccggaca cgggttgggc atctgatcgc 960 gaaatgcttg tagattatgt gcgtgtgtac cagtgtgatt ctgaagccga tgatggaaca 1020 ggctgtgcaa acgcaaccga cgccgttgat attgctattt caccgaccgc agatgtaggt 1080 gcgccatcac aagtagaata cgacctatat agtgacggtt tacaaacgcc tgcatttacc 1140 aacgccggcg taactcttgc tgcaaatgtt tggcaagaaa cagaaggcaa cgtagtaact 1200 accactggca acttgggcga cgaccatggc gatgcttggg aaattacctt taatggtact 1260 ggtaatgtat ctattgctat tgccgagcaa gacggcgtgg aaagctatgt gcgattaaat 1320 ggcggaacag cttggagtaa ctacggcgta ctagcgtttg atatgtatgt agatagcgtg 1380 gatgccgaaa ctggctttgt tgttaaaatg gacagtgttt accccaatgt aggtgctgta 1440 gatattgcta cacctgccgc aggtgagtgg acgcgagtgc acgtaaaagt ggctgatatt 1500 ttagctaacc caattgcagg tggcggtggc cttaacgttg cgcaagcggt taacttgttt 1560 gtacttgagc caacaggtac taaaacagca catgtatatg tagataatat ttctattagc 1620 tgcgcatata actctactgc aaaatcttgg cagggtgata aaacctgtga tgttgcagcg 1680 gtgcctactg ccgatacctc aggtatagat ttaagtggta acgagcttgt gatttttgat 1740 gagcaagagc cggatttttg gggttttggg cagttttctg ctggtggtgc tgaagtagca 1800 atgagcttct tagctgaccc agaagatgcg agtcacggta atgtgttggg ctttagctac 1860 ccagatagcc aaaacgttgc ctacttacaa tctgctacac cgcttaattt aagcgattgg 1920 gctgggggta ctatccaatt cgacatgtac gttattagcg agcctgctaa tgtaaattgg 1980 atgatgaaag tggattgtgt acacccttgt tcatctggcg atataccgct caccaccaat 2040 attgatggtg tagtgcctgc tgtgggtgta tggcaaacct atcgctttaa cttagatgca 2100 ttagttgctg gcaacccagg gttagatttg accaaagtgg atacgccgct agtaatattc 2160 cctgcatggg ataaccaaac cggtgctaac ttccgtatag ataacattaa atttgttcgg 2220 gctaactag 2229 <210> 170 <211> 881 <212> PRT <213> Microbulbifer degradans <400> 170 Met Gly Leu Thr Met Val Lys Asn Lys Leu Tyr Leu Ala Leu Ser Ile 1 5 10 15 Ala Ala Ala Thr Ser Le Le Thr Ala Cys Gly Gly Gly Gly Asp Ala Ala             20 25 30 Asp Asp Thr Val Lys Arg Asn Val Phe Ala Val Gly Asp Val Phe Lys         35 40 45 Thr Gln Glu Asp Gly Asp Ala Val Glu Ala Asp Val Ser Glu Asn Asp     50 55 60 Phe Gly Arg Gly Leu Thr Phe Ala Leu Glu Ser Gly Ser Thr Thr Ala 65 70 75 80 Asn Gly Glu Leu Val Phe Asn Ala Asp Gly Ser Phe Thr Tyr Thr Pro                 85 90 95 Asn Ala Asp Phe Ser Gly Lys Asp Ser Phe Thr Tyr Val Ala Thr His             100 105 110 Thr Ala Ser Gly Asp Thr Ala Ser Ala Leu Val Thr Ile Asn Val Ile         115 120 125 Ser Asp Phe Glu Thr Ile Glu Glu Ser Gly Trp Thr Leu Thr Trp Ser     130 135 140 Asp Glu Phe Asp Ser Leu Asp Ser Met Ala Trp Asp Ala Met Asn Ala 145 150 155 160 Ser Ala Ser Glu Gly Val Leu Ser Val Ser Ala Val Glu Gly Gln Thr                 165 170 175 Ser Tyr Val Lys Ser Thr Ala Ala Leu Gly Gln Ala Gly Arg Ile Glu             180 185 190 Ala Ser Ile Gln Leu Pro Asp Gly Lys Ser Leu Tyr Ser Gly Phe Gly         195 200 205 Leu Met Pro Met Ala Asp Met Phe Asp Gly Lys Asn Ala Leu Met Ala     210 215 220 Ile Glu Ser Ala Asn Asn Lys Ala Thr Ala Gly Gly His Trp Gly Ile 225 230 235 240 Gly Leu Val Asn Gly Val Glu Ile Asn Glu Pro Thr Asn Ala Val Val                 245 250 255 Arg Ala Glu Phe His Thr Tyr Ala Ile Glu Trp Asn Glu Ser Leu Ile             260 265 270 Arg Trp Tyr Ile Asp Asp Ile His Ile His Thr Val Asp Thr Leu Asn         275 280 285 Thr Trp Ser Tyr Asn Leu Ser Gly Asp Thr Val Val Ala Asp Thr Thr     290 295 300 Thr Lys Pro Phe Ser Gln Asp Leu Gln Ile Met Met Glu Leu Thr Ala 305 310 315 320 Ala Ser Ser Gly Leu Pro Asn Ala Met Leu Val Asp Phe Val Lys Val                 325 330 335 Tyr Glu Cys Asp Thr Ser Val Thr Asp Gln Ile Glu Ser Cys Ala Phe             340 345 350 Ala Ala Asp Glu Asn Val Asp Lys Leu Ala Ser Asn Arg Ile Glu Ser         355 360 365 Val Gly Glu Ile Val Thr Pro Leu Phe Thr Asp Glu Leu Thr Ser Leu     370 375 380 Ser Trp His Tyr Ser Asp Ala Glu Glu Ala Val Thr Phe Val Thr Glu 385 390 395 400 Gln Glu Ser Ala Val Pro Thr His Arg Gly Ile Val Ser Gln Pro Asp                 405 410 415 Val Met Glu Pro Val Ala Glu Val Ala Pro Val Asp Pro Val Glu Glu             420 425 430 Gly Glu Glu Gly Tyr Glu Glu Tyr Gln Ala Tyr Leu Ala Tyr Val Asp         435 440 445 Tyr Leu Asn Tyr Leu Glu Thr Leu Ala Phe Leu Asp Thr Val Asp Pro     450 455 460 Ser Arg Glu Arg Gly Ala Val Ile Arg Tyr Gln Ser Asp Ala Ser Thr 465 470 475 480 Trp Ser Asn Phe Ser Leu Asn Thr Pro Ser Leu Gly Leu Val Gly Lys                 485 490 495 Asp Ser Ala Leu Gln Phe Asp Met Tyr Ile Asp Ser Ala Ser Thr Thr             500 505 510 Thr Glu Thr Ile Glu Ile Arg Met Glu Thr Gly Trp Pro Phe Leu Gly         515 520 525 Thr Val Leu Leu Asn Val Ala Asp Leu Gln Leu Asp Thr Trp Val Thr     530 535 540 Tyr Asn Ile Pro Val Ser Asp Phe Leu Ala Asn Pro Phe Ile Thr Pro 545 550 555 560 Asp Trp Ala Val Gly Gln Asp Trp Phe Leu Gly Gly Asn Gly Val Glu                 565 570 575 Gly Gln Pro Leu Tyr Leu Asp Thr Asn Ser Ile Thr Lys Ala Ile Val             580 585 590 Val Gln Leu Ala Ala Pro Gly His Leu Val Phe Asp Asn Val Ala Ile         595 600 605 Thr Cys Val Ser Asn Glu Ser Cys Phe Gln Gly Pro Leu Ala Lys Gln     610 615 620 Pro Ile Val Lys Ala Gly Pro Ala Pro Ile Ile Tyr Glu Ala Glu Ala 625 630 635 640 Tyr Thr Ala Val Thr Gly Glu Val Gln Thr Glu Asp Thr Gln Asp Ala                 645 650 655 Gly Gly Gly Gln Asn Val Gly Tyr Ile Asp Ala Gly Glu Ala Leu Glu             660 665 670 Tyr Thr Ile Val Ala Pro Ile Asp Gly Thr Tyr Lys Phe Gln Tyr Arg         675 680 685 Leu Ala Ser Gly Leu Glu Ser Ala Ser Glu Phe Asp Val Ser Ile Asp     690 695 700 Asp Met Leu Ile Asp Gly Gln Ser Leu Pro Gly Thr Gly Gly Trp Gln 705 710 715 720 Val Trp Thr Thr Leu Glu Ser Gly Glu Phe Asp Leu Thr Ala Gly Glu                 725 730 735 His Ala Ile Val Phe Asn Phe Ala Gly Gly Met Asn Phe Asn Trp Phe             740 745 750 Ala Ile Val Pro Pro Pro Ile Ala Ile Phe Ile Glu Ala Glu Asp Tyr         755 760 765 Ser Ser Met Ala Gly Val Gln Leu Glu Asp Thr Ala Asp Glu Gly Gly     770 775 780 Gly Gln Asn Val Gly Tyr Ile Asp Ala Gly Asp Phe Leu Gln Tyr Asn 785 790 795 800 Val Glu Val Pro Ala Asp Gly Thr Tyr Phe Ile Glu Leu Arg Val Ala                 805 810 815 Ser Ser Gly Gly Ser Asp Gly Phe Thr Ile Thr Ser Asn Gly Ile Thr             820 825 830 Thr Ser Thr Ile Pro Val Ala Asp Thr Gly Gly Trp Gln Asn Trp Thr         835 840 845 Thr Gln Thr Val Glu Met Gln Leu Ser Ala Gly Gln Gln Thr Leu Arg     850 855 860 Phe Asp Phe Ile Gly Gly Ala Ile Asn Phe Asn Trp Ile Asn Val Thr 865 870 875 880 Asn      <210> 171 <211> 2646 <212> DNA <213> Microbulbifer degradans <400> 171 atggggttaa caatggttaa gaataaattg tatctagcgc tatctatagc ggctgctaca 60 agcctaacgg catgtggtgg tggcggggat gcggctgatg acactgttaa acgcaacgta 120 tttgccgtag gcgatgtgtt taaaacgcaa gaagatggcg atgcggttga ggcggatgta 180 agtgaaaacg attttggcag aggcctcacg ttcgcattag aaagcggtag caccacggct 240 aatggcgagc tggtattcaa tgccgatggc tcttttacgt atacacccaa tgcggacttc 300 tctggtaaag actcctttac ctatgtggcc acccacaccg catcgggcga tacagccagt 360 gccttggtga ctattaacgt aataagtgac tttgaaacca tcgaggaatc tgggtggacg 420 ctaacgtggt ccgacgaatt cgatagttta gatagcatgg cgtgggatgc gatgaacgca 480 tctgcttctg aaggtgtgtt gtcggtaagc gctgtagaag ggcaaacatc ttacgttaaa 540 agcaccgctg cactggggca ggctgggcgt attgaggcga gcattcagtt gcccgatggt 600 aaaagcttgt actctggctt tggcttaatg ccaatggccg acatgttcga tggtaaaaat 660 gcattaatgg ccatcgagag cgcgaacaat aaggcaactg ctggtggtca ttggggtatt 720 ggattagtaa atggtgttga aattaatgaa cccactaacg cagtagtacg tgcggaattt 780 catacctatg ccatagagtg gaatgagagt ctgattcgct ggtatataga tgatatacat 840 attcataccg tagataccct taatacctgg tcatacaatt taagcggcga taccgtggtt 900 gccgatacta ccactaagcc gtttagccaa gacctgcaaa taatgatgga gttaacggca 960 gcaagttcag gtttgccaaa tgcaatgctg gtagattttg taaaggttta tgagtgtgac 1020 acgtctgtta ccgatcaaat agaaagctgc gcattcgcgg ctgacgaaaa tgtggataag 1080 cttgcttcca accgtattga gtcggttggt gaaattgtta ccccattgtt cacagatgag 1140 cttacatcgt taagctggca ttactctgat gccgaagaag ctgttacttt tgttacagag 1200 caagaaagcg ctgtgcctac tcatagaggt attgtttctc agcccgacgt tatggagcct 1260 gtagcagaag ttgcaccagt ggaccctgta gaagaaggtg aagagggcta cgaggaatat 1320 caagcttact tagcttatgt ggactacctg aactatttag aaactctcgc atttttagat 1380 acagtggacc caagccgcga acgtggcgct gttattcggt atcaatcgga tgcaagcaca 1440 tggtctaatt ttagtttgaa tactccgagc ttaggcttag ttggaaaaga ttccgcgctg 1500 cagtttgata tgtatatcga tagcgcatct acaactaccg aaaccatcga aatacgcatg 1560 gaaaccggtt ggccattcct tggcactgtt ctgctaaacg ttgcagactt gcagctagat 1620 acttgggtga cgtacaacat tcccgttagc gactttttag ccaatccatt tattacaccc 1680 gattgggcag tgggccaaga ctggttcttg ggcggtaacg gtgttgaagg gcagcctctc 1740 tatttagata ccaattcaat tactaaagcc attgtggttc agctagcagc accagggcat 1800 ttagttttcg ataacgttgc aattacttgt gtttcgaatg aaagctgctt ccaggggcca 1860 ttagccaaac agcccatcgt aaaggccggt cctgctccca ttatttacga agcagaagct 1920 tacacggcgg taacgggtga agtgcaaacg gaagataccc aagacgcggg cggcggccaa 1980 aacgttggct atatcgacgc aggtgaagca ttggaataca ccattgttgc acctatcgac 2040 ggtacctata aattccaata tcgcttggca agtggcttag aaagcgcttc tgagtttgat 2100 gtttctatcg acgatatgct tatcgatggc caaagcctac ctggtactgg tggctggcaa 2160 gtgtggacca cattggagtc tggtgagttt gatctaactg ctggtgaaca cgctattgtg 2220 tttaattttg ctggcggtat gaactttaac tggtttgcaa ttgttccgcc ccctattgcg 2280 atttttatcg aagctgaaga ttactcgtca atggctggtg ttcagcttga agacaccgca 2340 gatgaaggcg gcggtcaaaa tgttgggtat atcgacgcag gtgatttcct tcaatacaac 2400 gtcgaagtgc cggctgatgg tacctacttc attgaattgc gtgtagccag cagtggtggt 2460 agcgatggct ttaccattac ttccaatggc attactacaa gcactattcc tgtagcggat 2520 accggtggtt ggcaaaattg gactactcaa actgtagaga tgcaattgtc tgctggtcaa 2580 caaacacttc gtttcgactt tatcggtggg gccattaact ttaactggat caatgtcacc 2640 aactaa 2646 <210> 172 <211> 1186 <212> PRT <213> Microbulbifer degradans <400> 172 Met Ile Leu Arg Leu Ile Arg Ala Ala Ile Tyr Ile Val Ala Phe Val   1 5 10 15 Ser Leu Ile Ala Cys Gly Gly Ser Ser Thr Ser Lys Pro Ala Gln Pro              20 25 30 Asp Ile Pro Asp Thr Asp Leu Pro Asp Thr Gly Thr Pro Glu Pro Glu          35 40 45 Asn Ser Asp Pro Val Val Gln Ser Thr Pro Ala Thr Ala Ile Ala Ala      50 55 60 Gly Asn Val Tyr Ser Tyr Gln Ile Met Ala Ser Asp Ala Asp Glu Ser  65 70 75 80 Asp Val Ile Ser Tyr Ala Ala Val Thr Leu Pro Ser Trp Leu Ala Phe                  85 90 95 Asp Pro Asp Thr Gly Ile Leu Thr Gly Thr Pro Gln Gln Ala His Ala             100 105 110 Gly Asn Ala Glu Val Val Leu Ser Tyr Ser Asp Gly Asn Val Thr Leu         115 120 125 Gln Gln Gln Phe Thr Ile Ala Val Ser Ala Ser Tyr Thr Glu Glu Pro     130 135 140 Pro Thr Pro Met Ser Arg Pro Thr Val Asn Ala Ala Asp Thr Ser Thr 145 150 155 160 Tyr Thr Ile Thr Ala Tyr Gly Ala Gly Ser Ile Ala Asp Ala Ile Asn                 165 170 175 Pro Ala Ser Tyr Gly Cys Val Tyr Asp Tyr Gly Asn Trp Ile Tyr Asn             180 185 190 Ala Gly Val Val Glu Pro Gly Val Ser Gly Cys Asp Pro Ile Gly Ala         195 200 205 Pro Thr Tyr Arg Thr Pro Gln Val Val Gly Glu Ala Ala Ser Val Pro     210 215 220 Thr Pro Thr His Lys Trp Trp Gly Ser Val Ser Phe Leu Gly Glu Met 225 230 235 240 Lys Ile Gly Asp Pro Ala Gly Ala Gly Tyr Ile Thr Pro Asp Pro Ile                 245 250 255 Thr Ala Arg Ile Ser Asn Lys Gly Val Arg Ile Met Gly Ile Pro Asn             260 265 270 Gly Leu Gly Ala Gln Gly Asn Gln Phe Ile Tyr Ser Val Pro Asp Pro         275 280 285 Phe Ser Glu Val Phe Asp Gly Ile Ala Val Ala Asn Ser Glu Tyr Ala     290 295 300 Asn Leu Glu Ala Tyr Leu Lys Ser Tyr Ser Asp Gly Thr Ala Thr Val 305 310 315 320 Gln Trp Gln Ser Gly Asn Leu Pro Val Met Gln Ala Thr Phe Val His                 325 330 335 Gly Ser Pro Tyr Val Phe Phe Lys Ala Tyr Arg Gly Asn Met Val Leu             340 345 350 Arg Thr Lys Ala Ala Asp Gly Gly Glu Lys Gly Thr Phe Tyr Asn Glu         355 360 365 Asn Asn Ser Leu Gly Ile Trp Thr Ser Val Ala Gly Asn Lys Asn Asp     370 375 380 Phe Leu Ile Thr Gly Glu Gly Glu Thr Val Phe Asn Asn Ile Glu Thr 385 390 395 400 Asp Thr Ile Thr Leu Thr Asn Ala Ala Asn Glu Phe Thr Leu Thr Leu                 405 410 415 Leu Pro Thr Ala Gly Ala Gly Thr Pro Ser Ser Thr Val Ile Gln Ala             420 425 430 Phe Glu Asp Ser Ala Arg Ala Val Val Ala Lys Val Asp Ile Gln Tyr         435 440 445 Ser Val Asp Arg Thr Asn Asn Met Val Thr Val Thr His Thr Tyr Lys     450 455 460 Asn Glu Ser Asn Thr Pro Val Gln Thr Leu Ala Gly Leu Leu Pro Met 465 470 475 480 His Trp Lys Tyr Ser Asp Thr Ala Leu Ser Gly Tyr Lys Thr Arg Ser                 485 490 495 Ala Arg Gly Met Val Gln Phe Ala His Ile Asp Ser Phe Ser Tyr Thr             500 505 510 Ile Pro Tyr Val Gly Val Leu Pro Tyr Leu Pro Ser Ser Val Gly Asp         515 520 525 Phe Asp Ser Ser Val Leu Ala Gly Leu Val Gln Ala Phe Val Ala Glu     530 535 540 Gly Pro Glu Asn Trp Asn Pro His Thr Asp Thr Tyr Trp Ser Gly Lys 545 550 555 560 Ala Phe Asn Lys Val Ala Glu Leu Ser Ala Ile Ala Arg Ser Val Gly                 565 570 575 Met Thr Ser Glu Ala Asp Thr Leu Leu Asn Trp Leu Lys Ala Glu Leu             580 585 590 Gln Asp Trp Phe Ser Ala Asn Thr Asn Gly Ser Leu Asp Glu Lys Lys         595 600 605 Tyr Phe Val Tyr Asp Ala Glu Trp Asn Thr Leu Leu Gly Leu Glu Glu     610 615 620 Ser Phe Ala Ala His Gln Gln Leu Asn Asp His His Phe His Tyr Gly 625 630 635 640 Tyr Phe Val Arg Ala Ala Ala Glu Ile Cys Arg Val Asp Ala Ser Trp                 645 650 655 Cys Gly Ala Asp Gln Tyr Gly Pro Met Val Glu Leu Leu Ile Arg Asp             660 665 670 Tyr Ala Gly Ala Lys Asp Asp Thr Met Phe Pro Tyr Val Arg Asn Phe         675 680 685 Asp Pro Ala Asn Gly Phe Ser Trp Ala Ser Gly Ser Ala Asn Phe Val     690 695 700 Leu Gly Asn Asn Asn Glu Ser Thr Ser Glu Ala Ala Asn Ala Tyr Gly 705 710 715 720 Ala Ile Ile Leu Tyr Gly Leu Ile Thr Gly Asp Asn Glu Leu Val Glu                 725 730 735 Arg Gly Met Tyr Leu His Ala Ser Ser Ser Val Ala Tyr Trp Glu Tyr             740 745 750 Trp Asn Asn Ile Asp Arg Tyr Leu Gly Ala Asp Ala Asp Arg Asp Asn         755 760 765 Phe Pro Ser Gly Tyr Asp Lys Leu Thr Thr Ser Ile Ile Trp Gly His     770 775 780 Gly Gly Val Phe Ser Thr Trp Phe Ser Gly Ala Tyr Ala His Ile Leu 785 790 795 800 Gly Ile Gln Gly Leu Pro Thr Asn Pro Leu Ile Phe His Val Gly Leu                 805 810 815 His Pro Glu Tyr Met Glu Asp Tyr Val Ala Leu Gly Leu Ser Glu Ser             820 825 830 Ser Asn Asn Lys Pro Ser Gly Leu Ile Asp Asp Gln Trp Arg Asp Ile         835 840 845 Trp Trp Asn Leu Trp Ala Leu Thr Asp Ala Glu Ala Ala Ile Ala Asp     850 855 860 Tyr Asn Thr Val Gly Ser Asn Tyr Ala Pro Glu Phe Gly Glu Thr Lys 865 870 875 880 Ala His Thr Tyr His Trp Leu His Thr Trp Asn Ala Leu Gly His Leu                 885 890 895 Lys Thr Gly Thr Gly Glu Leu Thr Val Asn Asp Pro Ala Ala Leu Val             900 905 910 Phe Glu Lys Asp Gly Ile Lys Thr Tyr Val Ala Tyr Asn Phe Ser Gly         915 920 925 Thr Pro Lys Thr Ile Leu Ala Ser Asp Gly Phe Glu Phe Ile Ala Gln     930 935 940 Pro Asn Asp Phe Thr Val Val Thr Thr Ala Asp Asn Asn Pro Asp Asp 945 950 955 960 Thr Gln Pro Pro Thr Leu Pro Ala Asn Leu Gln Ala Leu Asn Leu Thr                 965 970 975 Gln Thr Ser Leu Asp Val Lys Trp Asp Ala Ser Thr Asp Asn Tyr Arg             980 985 990 Met Ala Gly Tyr Val Val Gln Val Leu Gln Ala Asp Thr Leu Ile Glu         995 1000 1005 Glu Thr Ser Ser Ile Ala Ser Ile Ala Ser Phe Asn Asn Leu Thr Ala    1010 1015 1020 Ser Thr Ser Tyr Thr Ile Gln Val Lys Ala Lys Asp Arg Ser Gly Asn 1025 1030 1035 1040 Glu Thr Ala Trp Val Ser Ile Thr Val Thr Thr Pro Ser Glu Thr Asp                1045 1050 1055 Asp Leu Leu Pro Thr Leu Asp Gly Gly Val Tyr Ser Ala Asn Val Gly            1060 1065 1070 Pro Asn Ser Ala Asp Leu Ser Trp Ala Ala Ala Thr Asp Asp Arg Gly        1075 1080 1085 Ile Ala Ser Tyr Thr Ile Glu Val Gln Val Gly Gly Ala Val Phe Val    1090 1095 1100 Thr Glu Thr Val Phe Asp Thr Ser Tyr Ala Leu Ser Gly Leu Thr Glu 1105 1110 1115 1120 Ala Thr Glu Tyr Asn Val Ala Val Tyr Ala Thr Asp Thr Gly Gly Gln                1125 1130 1135 Gln Ser Ala Thr Ile Ser Gly Ile Val Asn Thr Thr Ser Asn Pro Phe            1140 1145 1150 Gly Ser Gly Cys Glu Leu Ile Cys Ala Ser Ala Thr Ser Ser Ser Ser        1155 1160 1165 Val Thr Phe Thr Val His Gln Ala Gly Ala Val Asp Ile His Tyr Leu    1170 1175 1180 Val asn 1185 <210> 173 <211> 3558 <212> DNA <213> Microbulbifer degradans <400> 173 atgatactgc gactgatacg tgccgcaatt tacattgtgg cgtttgtttc tcttatcgcc 60 tgtgggggct caagcacatc caagcccgcg caaccggata ttcccgatac ggatctgcct 120 gacacgggaa cacccgaacc cgaaaacagc gatccagttg ttcaatctac gccggctaca 180 gccattgcgg cgggcaacgt gtatagctat caaataatgg ctagcgacgc agacgaatcg 240 gatgtcatta gctatgcggc ggttactttg cctagctggc tggcgttcga cccagatacc 300 ggaatactta ccggcacgcc ccagcaagct catgctggta atgctgaagt ggtgcttagc 360 tatagtgacg gcaacgtaac cctgcagcag caatttacaa ttgcagttag cgcgagctac 420 accgaagagc caccaacacc gatgagtcgc ccaaccgtaa atgcggcgga taccagtact 480 tacactatta ctgcctacgg cgcgggcagt attgcggatg cgattaaccc tgctagctat 540 ggctgtgtgt acgattacgg taattggatt tataacgccg gcgttgtgga gccaggagta 600 agcggctgtg accctatagg cgcaccaacc taccgtacac cgcaggtggt tggggaagcg 660 gcaagcgtgc caaccccaac gcataaatgg tggggctcgg tttctttttt aggtgaaatg 720 aaaataggtg acccggcagg cgcaggttac ataacgccag acccaataac tgcacgtatc 780 tcgaataaag gcgtgcgtat tatgggcata cccaacggct tgggcgcgca aggcaaccag 840 tttatttact ctgtacccga cccttttagc gaggtattcg atggaatagc ggtagcgaat 900 agcgaatatg ccaacctaga agcgtattta aaatcctaca gcgatggcac cgctaccgtg 960 caatggcaga gtggcaacct gccggttatg caggccacat ttgtacacgg ctccccctat 1020 gtgtttttta aagcctatcg cggcaacatg gtgttgcgca ccaaagctgc agacggcggt 1080 gaaaaaggca cgttttataa tgaaaataat agtttaggta tttggaccag tgtagcgggt 1140 aacaaaaatg actttttaat taccggtgaa ggcgaaacgg tttttaacaa tatcgaaacc 1200 gataccatta cgttaaccaa tgcagcgaac gaatttacct taacgttatt acctacagcg 1260 ggcgccggta caccttcaag tactgttatt caggcgtttg aagatagcgc ccgtgcagtg 1320 gttgccaaag tagatattca atactcggta gaccgtacca ataacatggt aacggttact 1380 catacctata aaaatgaaag caatacgcca gtgcaaaccc tcgcggggtt actgcccatg 1440 cattggaagt attccgatac agcattaagc ggctacaaaa cccgaagtgc gcgcgggatg 1500 gtgcaatttg cccatattga ttcgtttagc tacaccattc cttatgtggg agtattaccg 1560 tatttaccgt catcggtagg ggatttcgat tcctctgtgc tcgcaggctt agtgcaggcg 1620 tttgtagctg aagggccaga aaattggaac ccgcacaccg atacctattg gtcgggcaaa 1680 gcgtttaata aagttgccga gttatcggca atagcgcgct cggtaggtat gacaagcgaa 1740 gcagatacgc tgttaaattg gcttaaagcc gaactgcaag attggtttag cgccaacacc 1800 aacggcagtt tggatgagaa aaaatacttt gtgtacgatg ccgagtggaa taccttgctt 1860 ggcctagaag aatcttttgc tgcacaccaa caactaaacg atcaccactt tcactacggc 1920 tattttgtac gcgccgctgc agaaatatgc cgagtagatg caagctggtg tggcgcagac 1980 cagtatggtc ccatggtgga actgcttatt cgcgattacg ccggtgccaa agacgatact 2040 atgttccctt acgtgcgtaa cttcgacccc gccaatggct tttcttgggc atcgggtagt 2100 gccaactttg tgctgggtaa caacaacgaa tccacatccg aagcggccaa tgcctatggt 2160 gcaattattc tgtacgggtt aataactggc gataatgagt tagtggagcg cggtatgtat 2220 ttgcacgcgt cttcgtcggt ggcctactgg gaatattgga acaacatcga ccgctacttg 2280 ggggccgatg ccgaccgcga taattttcca tcgggttacg acaaactaac tacctctatt 2340 atttgggggc atggcggcgt gttctctacc tggttcagtg gcgcttacgc tcatattctt 2400 ggtattcaag ggctgcctac caacccgctt atttttcatg tgggcttaca ccccgagtat 2460 atggaagact atgtggcact gggtttaagt gaatcgagca acaataaacc ttcggggtta 2520 atcgacgatc agtggcgcga tatttggtgg aacctatggg ccttgactga tgcagaagcg 2580 gccatcgccg attacaacac ggtaggcagt aactatgcgc cagagtttgg tgaaaccaag 2640 gcgcacacct accactggtt gcacacgtgg aatgcgctcg gccatttaaa aaccggcact 2700 ggcgagctaa cggtaaacga ccccgctgca ttagtgtttg aaaaggacgg aataaaaacc 2760 tacgtagcgt ataactttag cggtacacca aaaactattt tggctagcga tggctttgag 2820 tttattgcgc agcccaatga ttttaccgtg gtgaccaccg ccgataacaa ccccgacgac 2880 acgcaaccgc ctacactacc ggcaaacttg caagcgctta accttaccca aacgagcttg 2940 gatgttaaat gggatgcatc taccgataac taccgtatgg caggttatgt agtgcaggta 3000 ttacaagccg atacattaat agaagaaacg tcttctatag ccagcatcgc ctcgtttaat 3060 aacttaacgg ccagcacaag ttacaccatt caagttaaag ctaaggatcg ctctggcaat 3120 gaaaccgcat gggtaagcat tacggtaacc acgcccagcg aaaccgacga tttactacca 3180 acacttgatg gcggtgtata tagcgcaaac gtggggccta actctgcgga tttaagctgg 3240 gcggcagcaa cagatgatcg cggtatagca agttacacca tagaggtgca ggttggcggc 3300 gcagtatttg taactgaaac agtgtttgat acttcgtatg cgcttagcgg attgaccgaa 3360 gcaaccgaat acaatgtggc ggtttatgct acagataccg gcggtcaaca atctgccact 3420 attagcggta ttgttaatac caccagcaat ccatttggca gcggttgcga attgatttgt 3480 gccagcgcta cctctagctc atctgttacg tttactgtgc atcaagcggg cgcggtagat 3540 attcactact tagtgaac 3558 <210> 174 <211> 371 <212> PRT <213> Microbulbifer degradans <400> 174 Met Gly Ser Ser Phe Leu Thr Cys Tyr Lys Pro Ser Lys Glu Val Ile 1 5 10 15 Thr Val Lys Arg Ala Ala Ile Asn Leu Val Leu Ile Pro Gly Leu Ala             20 25 30 Val Ser Met Gly Thr Leu Ser Ser Ser Ala Phe Ala Gln Ser Thr Cys         35 40 45 Ser Val Asp Tyr Arg Val Glu Ser Asp Trp Gly Ala Gly Ala Thr His     50 55 60 Lys Val Leu Val Thr Asn Thr Gly Ala Pro Ile Asn Gly Trp Gln Met 65 70 75 80 Ser Trp Thr Phe Ser Gly Asn Glu Gln Ile Thr Asn Leu Trp Asn Gly                 85 90 95 Met Phe Thr Gln Ser Ser Gln Ser Val Ile Val Asp Ser Leu Ser Trp             100 105 110 Asn Ala Gln Leu Asn Thr Gly Ala Thr Ala Glu Val Gly Phe Asn Ile         115 120 125 Asn Ser Pro Ser Gly Gln Leu Pro Asp Val Tyr Leu Asn Gly Val Asn     130 135 140 Cys Ser Asn Pro Gly Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu 145 150 155 160 Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu                 165 170 175 Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu Pro Glu             180 185 190 Pro Glu Pro Glu Pro Glu Leu Ala Tyr Ser Leu Asp Ala Thr Ala Ser         195 200 205 Phe Leu Asn Phe Val Ser Ser Lys Lys Thr His Val Leu Glu Thr His     210 215 220 Arg Phe Asp Val Leu Ser Gly Gly Ile Ser Thr Ala Gly Glu Ala Gln 225 230 235 240 Leu Val Ile Asp Leu Asn Ser Val Asn Thr Gly Ile Asp Val Arg Asn                 245 250 255 Gly Arg Met Arg Asp Tyr Leu Phe Glu Thr Ala Thr Tyr Ser Val Ala             260 265 270 Thr Val Thr Val Pro Val Asp Leu Ala Ala Val Ala Gly Leu Ala Val         275 280 285 Gly Glu Asp Met Leu Val Asp Val Ser Ala Thr Leu Asp Leu His Gly     290 295 300 Val Pro Gly Val Ile Asp Thr Gln Leu Asn Val Gln Arg Leu Ser Ala 305 310 315 320 Thr Arg Ile Met Val Gln Asn Gln Ser Pro Leu Leu Ile Lys Ala Ala                 325 330 335 Asp Tyr Ser Leu Glu Ala Gly Ile Glu Thr Leu Arg Asn Leu Ala Ser             340 345 350 Leu Asn Val Ile Ser Thr Thr Val Pro Val Asp Phe Val Leu Phe Tyr         355 360 365 Glu ala pro     370 <175> 175 <211> 1116 <212> DNA <213> Microbulbifer degradans <400> 175 atgggaagca gttttctcac ctgttataaa cctagcaaag aggtaataac cgtgaagcga 60 gcagcaataa acttggtgct gatacccggg ctagccgtaa gtatggggac gttgtcgtct 120 tctgcttttg cacaaagcac ttgtagtgtg gactatcgag tagagtccga ctggggtgca 180 ggggctacgc acaaagtatt agttactaac actggagccc ccataaacgg gtggcaaatg 240 tcatggactt tttctggcaa cgagcaaatc actaaccttt ggaatggcat gttcacccaa 300 agctctcaaa gtgtgattgt tgatagtctg tcgtggaatg cccagctaaa taccggtgcc 360 accgcagaag tggggtttaa tattaactct ccttcagggc agttgccgga tgtgtatttg 420 aacggcgtaa attgtagtaa ccctggtgag ccagagccag agccagagcc agagccagag 480 ccagagccag agccagagcc agagccagaa cctgagcctg agccagaacc tgagccagaa 540 cctgagccag aacctgagcc agaacctgag ccagagccag agccagagcc agaattggcc 600 tattcattag atgcgactgc atctttctta aactttgtta gctcaaagaa aactcacgtt 660 cttgaaacac accgttttga cgtgctttct ggcggtatta gtactgcggg tgaggcacaa 720 cttgttatcg atcttaacag tgtgaacaca ggtatagacg tgcgcaacgg acgtatgcgc 780 gattacctgt ttgagactgc aacctattct gtagcaaccg tgactgtgcc tgtagatcta 840 gccgcagtag caggtttagc cgtgggcgaa gatatgctgg tggatgtatc cgcaactttg 900 gatcttcacg gtgtgccagg cgttatcgat acgcagctaa atgtacagcg cttatcggca 960 actcgcatta tggttcagaa ccaatctcca ctattaataa aggcagccga ttattcgctt 1020 gaggcgggta tcgagacatt gcgtaatctc gcgagcctaa atgttattag caccacggtg 1080 ccagtggatt ttgttttgtt ctacgaagcg ccttaa 1116 <210> 176 <211> 1042 <212> PRT <213> Microbulbifer degradans <400> 176 Met Lys Lys Ile Phe Lys Ile Ser Ala Leu Ser Leu Gly Phe Ser Ile   1 5 10 15 Ala Gly Ala Ala Ser Ala Ala Asp Leu Cys Asn Val Thr Tyr Glu Ala              20 25 30 Val Asn Ser Trp Gly Ser Gly Ala Gln Gln Ala Val Thr Val Val Asn          35 40 45 Asn Gly Pro Ala Leu Asn Ala Trp Gln Leu Ser Trp Thr Phe Asn Gly      50 55 60 Ser Glu Asn Ile Asp Asn Leu Trp Asp Gly Val Leu Ser Gln Thr Gly  65 70 75 80 Ala Asn Val Thr Val Asn Asn Ala Gly Tyr Asn Gly Ser Val Gly Thr                  85 90 95 Gly Gly Gln Phe Ser Phe Gly Phe Thr Val Ser Gly Trp Ser Glu Asn             100 105 110 Phe Pro Thr Glu Phe Tyr Leu Asn Gly Glu Ala Cys Ser Gly Ala Val         115 120 125 Asp Pro Asn Pro Asn Pro Asn Pro Glu Pro Thr Asp Gly Ala Val Trp     130 135 140 Glu Leu Asn Ser Ala Asp Ser Val Phe Ser Phe Val Thr Val Lys Lys 145 150 155 160 Glu His Val Ala Glu Val Gln Thr Phe Thr Ala Tyr Asn Ala Thr Val                 165 170 175 Asp Ser Asp Gly Val Ala Thr Leu Ala Ile Asp Leu Asn Ser Ala Glu             180 185 190 Thr Asn Ile Asp Ile Arg Asn Glu Arg Phe Arg Asn Val Leu Phe Glu         195 200 205 Thr Ala Phe Leu Pro Thr Leu Tyr Tyr Ser Val Gln Leu Asp Met Ala     210 215 220 Ser Leu Ser Ala Leu Ala Val Gly Asp Ala Gln Thr Gln Thr Leu Gly 225 230 235 240 Gly Thr Leu Thr Leu His Gly Val Gln Ala Val Val Glu Ala Glu Val                 245 250 255 Leu Val Val Lys Thr Ser Ala Thr Asp Leu Thr Val Ser Thr Ser Lys             260 265 270 Pro Ile Leu Ile Lys Ala Ala Asp Phe Asp Leu Val Ser Gly Val Glu         275 280 285 Ser Leu Arg Ala Leu Ala Ser Leu Ser Ser Ile Gly Gln Thr Val Pro     290 295 300 Val Tyr Phe Arg Leu Asp Phe Asp Ala Ala Asp Pro Gln Val Thr Asn 305 310 315 320 Ala Val Ala Val Pro Ala Thr Pro Ala Ala Pro Thr Ser Leu Thr Ala                 325 330 335 Asp Phe Thr Glu Ser Ser Gly Ila Ala Ala Leu Asn Trp Asn Asp Ala             340 345 350 Ser Asn Asn Glu Thr Glu Phe Leu Val Arg Arg Arg Glu Ala Ser Thr         355 360 365 Gly Tyr Trp Ser Arg Leu Thr Glu Val Asn Ala Asn Ser Thr Leu Leu     370 375 380 Asp Asp Leu Leu Leu Glu Glu Asp Thr Tyr Asp Tyr Lys Val Ile Ala 385 390 395 400 Leu Asn Asn Gly Val Pro Ser Ala Pro Ser Pro Val Ala Thr Val Val                 405 410 415 Ala Thr Thr Asn Pro Asn Pro Glu Pro Leu Thr Gly Glu Glu His Tyr             420 425 430 Gln Ala Lys Cys Ala Ser Cys His Gly Asp Asp Ala Ser Gly Gly Val         435 440 445 Val Gly Val Ala Leu Asn Thr Glu Arg Asp Leu Thr Val Met Leu Asn     450 455 460 Thr Ile Val Thr Arg Met Pro Pro Gly Glu Ala Asp Asn Cys Asp Gln 465 470 475 480 Glu Cys Ala Glu Ala Ile Gly Gly Tyr Ile Gln Thr Thr Phe Trp Asn                 485 490 495 Gly Gly Glu Pro Glu Pro Glu Leu Ala Cys Asp Thr Val Thr Tyr Gly             500 505 510 Ala Arg Gln Leu Lys Leu Leu Thr Lys Ala Glu Tyr Gln Arg Ser Val         515 520 525 Glu Asp Leu Val Gly Ile Asp Tyr Asn Val Ala Ser Gly Leu Ala Glu     530 535 540 Asp Asn Ile Ile Gly Tyr Phe Val Asn Asn Thr Thr Lys Val Val Val 545 550 555 560 Pro Thr Val Tyr Asp Gln Tyr Leu Thr Val Ala Glu Glu Ile Ala Gln                 565 570 575 Trp Ser Ala Asp Arg Asn Phe Ala Gly Ala Leu Thr Cys Gly Thr Asn             580 585 590 Phe Asn Gln Thr Cys Ala Asn Gln Phe Val Asn Asn Phe Ala Pro Lys         595 600 605 Val Phe Arg Arg Ala Leu Ser Ser Asp Glu Ala Ala Ala Tyr Leu Ala     610 615 620 Ile Ala Asn Gly Ser Ala Thr Asn Gly Asp Val Lys Ala Gly Ile Gln 625 630 635 640 Leu Ala Met Glu Gly Leu Phe Ser Ser Pro Gln Phe Val Tyr Arg His                 645 650 655 Glu Leu Gly Glu Ala Asn Pro Asn Asn Asn Ala Ile Asp Ser Asp Ala             660 665 670 Phe Glu Leu Thr Ser Tyr Glu Met Ala Thr Trp Leu Ser Tyr Thr Tyr         675 680 685 Ala Gly Thr Thr Pro Asp Ala Ile Ala Met Gln Lys Ala Ala Asn Asn     690 695 700 Gln Leu Arg Thr Asp Ala Glu Ile Arg Ala Glu Ala Gln Arg Leu Leu 705 710 715 720 Glu Gly Ala Gly Ala Lys Gln Lys Met Gly Asp Phe Val Ala Ser Trp                 725 730 735 Leu Gly Thr Asp His Ile Ala Asn Ala Pro Lys Asp Ala Ser Val Trp             740 745 750 Pro Gly Phe Asp Ala Leu Ile Pro His Leu Gln Thr Glu Ile Arg Glu         755 760 765 Met Phe Ser Tyr Val Met Leu Glu Pro Thr Glu Ser Phe Ala Ser Val     770 775 780 Tyr Asn Ala Asn Tyr Thr Phe Val Asn Gly Pro Leu Ala Gln His Tyr 785 790 795 800 Gly Ile Asn Gly Val Ser Gly Asn Glu Phe Gln Lys Val Thr Thr Thr                 805 810 815 Asp Arg Gly Gly Ile Leu Ala Asn Gly Ala Phe Met Ala Arg Trp Gly             820 825 830 Glu Ser Val Glu Ser Ser Pro Ile Arg Arg Ser Val Arg Val Arg Arg         835 840 845 Arg Met Leu Cys Gln Asp Gln Pro Asp Pro Pro Gly Asn Val Asn Ile     850 855 860 Gly Arg Glu Asn Ala Ala Asp Glu Phe His Glu Ala Leu Ala Asp Pro 865 870 875 880 Thr Thr Thr Asn Arg Glu Arg Tyr Glu Leu Leu Thr Ser Gly Glu Thr                 885 890 895 Cys Ala Thr Cys His Gln Glu Trp Ile Asn Pro Leu Gly Phe Gly Met             900 905 910 Glu Asp Phe Thr Ala Val Gly Thr Arg Arg Val Thr Asp Leu Asn Gly         915 920 925 Asn Thr Ile Asp Ala Ser Gly Gln Leu Tyr Ala Pro Glu Asn Leu Asn     930 935 940 Asp Lys Asp Val Phe Ile Asn Phe Asn Gly Thr Gln Gly Leu Gly Ala 945 950 955 960 Leu Leu Thr Thr Leu Pro Ser Ala Gln Ser Cys Leu Pro Gln Asn Leu                 965 970 975 Phe Arg Tyr Ser Val Gly Val Gly Val Glu Gly Leu Asp Asp Asn Pro             980 985 990 Glu Gly Asn Glu Leu Val Pro Ala Glu Arg Asp Gly Tyr Ala Cys Glu         995 1000 1005 Val Lys Asn Leu Thr Ser Thr Met Leu Glu Gln Ser Pro Arg Ala Met    1010 1015 1020 Leu Glu Gly Met Gly Ser Met Gln Ala Val Arg Tyr Arg Lys Ala Trp 1025 1030 1035 1040 Ala arg         <210> 177 <211> 3129 <212> DNA <213> Microbulbifer degradans <400> 177 atgaaaaaaa tattcaagat ttcagcgctc tcgctaggtt tctcaattgc tggcgctgca 60 tctgcagctg atttgtgcaa tgtaacctac gaagcggtaa atagctgggg ctccggtgcc 120 cagcaagcgg taaccgttgt aaataacggc cctgctttaa atgcctggca gttaagctgg 180 acatttaacg gcagcgaaaa tatagacaac ctatgggatg gcgtgctttc gcaaactggt 240 gcgaatgtta ccgttaacaa tgctggctat aacggcagtg ttggtactgg cggtcaattt 300 agcttcggtt ttaccgtaag cggttggagc gaaaactttc ccacagagtt ttacctaaat 360 ggtgaagcgt gtagtggagc tgttgaccct aatcccaatc ctaacccaga gccaactgat 420 ggcgctgtgt gggagttaaa ctcagccgat tcagtgttta gctttgtaac tgttaaaaaa 480 gagcacgttg ccgaagttca aacgtttact gcctacaacg caacagttga tagcgatggc 540 gttgcaacgc ttgctataga cttaaacagt gcagaaacga acattgatat acgcaacgag 600 cgcttccgca atgtattgtt tgaaacagct ttccttccta ctctgtacta cagtgttcag 660 ctagatatgg cttcactgtc tgcattggcg gttggcgatg ctcaaacgca aacgcttggt 720 ggcacgctaa cactacacgg tgtacaagca gtggttgagg ctgaagtgtt ggttgttaaa 780 actagcgcta ccgacttaac cgtatcaacg tctaagccta ttttaattaa ggctgccgat 840 tttgatttgg taagcggtgt agagtctttg cgagccttgg ctagtctttc aagtattggt 900 cagacggtac ctgtttactt ccgtttagac ttcgatgctg cagacccgca agtaactaat 960 gcggttgctg ttcctgctac tcctgcggcg ccaacgtctc taactgcaga cttcacagaa 1020 agcagtggta ttgccgcgtt gaactggaat gatgcaagca ataacgaaac cgagtttttg 1080 gttcgtcgtc gtgaagcgtc aaccggttac tggtcaagac tgaccgaagt taacgcgaac 1140 agtactttgt tagatgatct tctgttagaa gaagacacct acgactacaa agtgattgca 1200 ttgaacaacg gtgtgccttc tgcgccatcg ccagttgcaa ctgttgtagc tactaccaac 1260 cctaacccag aaccattgac tggcgaagag cactatcaag ctaagtgcgc aagctgtcac 1320 ggtgatgacg ctagcggtgg tgtagttggt gttgctttga atactgagcg cgatttaacc 1380 gttatgctga acaccattgt tactcgcatg cctccaggcg aagcggataa ctgtgatcaa 1440 gagtgtgcgg aagcaattgg tggttatatt caaactactt tctggaacgg cggtgagcca 1500 gagccagaat tagcttgtga cactgttact tacggtgctc gtcagttgaa gctacttacc 1560 aaagctgagt atcaacgttc tgttgaagac ttagttggta ttgattacaa cgtagcaagc 1620 ggcttagcag aagataacat tattggttac ttcgttaata acaccaccaa agtggttgtg 1680 ccaacggttt acgatcagta tctcacagta gctgaagaaa tcgcgcagtg gtctgctgac 1740 agaaactttg cgggtgcatt aacttgtggc actaacttta accaaacctg tgcgaatcag 1800 tttgttaaca acttcgctcc aaaagtattc cgacgcgcgc tttctagcga tgaagctgcg 1860 gcttacttgg cgattgctaa cggcagcgct accaacggtg atgttaaagc gggtattcaa 1920 cttgcgatgg aaggcttgtt ctcttctccg caatttgtat atagacacga gcttggtgaa 1980 gccaatccaa ataacaatgc tatcgactct gacgcgtttg aattaacgtc ttatgaaatg 2040 gctacttggt tgtcttacac ttatgccggt accacaccag atgcaattgc tatgcaaaaa 2100 gctgcaaaca atcagttgcg cacagacgcg gaaattcgcg ccgaagctca acgtttgtta 2160 gaaggtgctg gtgcgaagca aaaaatgggt gacttcgttg ctagctggtt aggtactgac 2220 cacattgcaa acgcacctaa agatgcttct gtatggcctg gttttgatgc gttaattccg 2280 catttacaaa ctgaaatacg cgaaatgttc tcgtacgtaa tgttagagcc aactgaaagc 2340 tttgcttcgg tttacaacgc taactatacc ttcgtgaacg gaccattggc gcaacactac 2400 ggcattaacg gtgtaagcgg taatgaattc caaaaagtaa caactaccga tcgcggtggc 2460 attttggcga acggtgcgtt tatggcgcgc tggggtgaaa gtgtagagtc ttcaccaatt 2520 cgtcgttctg tgcgcgtacg ccgtcgtatg ctttgtcagg atcaaccaga tccccctggc 2580 aacgtaaaca tcggtcgtga aaacgctgca gacgaattcc acgaggcgtt ggcagatcct 2640 actacgacta accgtgagcg ttatgagctg ttaacatcag gtgaaacttg tgctacttgt 2700 caccaagagt ggattaaccc tctcggcttt ggtatggaag atttcactgc ggttggtacg 2760 cgtcgtgtga cggatctaaa tggcaatact attgatgcaa gcggtcaatt gtacgcgcca 2820 gaaaacctca acgataaaga cgtctttatt aactttaacg gtactcaagg tttaggtgcg 2880 ttactgacta ccttgccaag tgcgcagtct tgtttgccac aaaacttgtt ccgttattcc 2940 gtgggcgttg gtgtagaagg gttggatgat aatccagaag gcaacgagct agttcctgca 3000 gaacgggatg gctatgcctg tgaagttaaa aacctgacga gcactatgtt agagcaaagt 3060 ccgcgagcaa tgctggaagg catgggttcc atgcaagcgg tacgttaccg caaagcgtgg 3120 gcgcgttaa 3129 <210> 178 <211> 933 <212> PRT <213> Microbulbifer degradans <400> 178 Met Tyr Pro Leu Pro Phe Asn Ser Lys Ile Val Ile Ser Leu Gly Ala 1 5 10 15 Met Thr Leu Ala Leu Ala Thr Gln Gln Ala Gln Ala Leu Ser Cys Thr             20 25 30 Val Ser Ala Asp Ser Trp Asn Ser Gly Tyr Thr Ala Asn Val Thr Val         35 40 45 Val Asn Asp Ser Ser Tyr Ser Ile Asn Ser Trp Asp Val Thr Leu Gly     50 55 60 Phe Asn Gln Pro Pro Ser Val Ser Ala Gly Trp Asn Ala Asn Val Ser 65 70 75 80 Thr Val Gly Thr Thr Val Met Ala Ser Asn Val Gly Tyr Asn Gly Asn                 85 90 95 Leu Ser Pro Gly Gln Ser Thr Ser Phe Gly Phe Gln Gly Ala His Asn             100 105 110 Gly Asn Phe Glu Leu Pro Ser Cys Ala Gly Gly Met Thr Ser Ser Ser         115 120 125 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser     130 135 140 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 145 150 155 160 Ser Ser Ser Asn Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser                 165 170 175 Thr Ser Ser Ser Ser Gly Ser Gly Gly Ala Asn Thr Val Thr Ile Glu             180 185 190 Leu Glu Asn Leu Ser Gly Gln Asn Gly Phe Ser Pro Phe Ser Val Gln         195 200 205 Asn Asp Ser Ser Ala Ser Gly Gly Gln Tyr Ile Val Trp Pro Asn Asn     210 215 220 Gly Asp Gln Leu Leu Ser Gly Ala Ser Asp Gly Gln Ser Gly Thr Leu 225 230 235 240 Ala Val Ser Phe Glu Leu Ser Gln Thr Ala Asn Val Ser Phe Asp Ile                 245 250 255 Arg Ala Ser Leu Ala Asn Gly Asn Asp Asp Ser Phe Tyr Tyr Lys Leu             260 265 270 Asp Ser Gly Val Trp Ser Thr Gln Asn Asn Thr Ser Thr Ser Gly Phe         275 280 285 Glu Thr Leu Ser Pro Thr Thr Phe Asn Gly Val Ser Ala Gly Val His     290 295 300 Thr Leu Tyr Ile Gln Arg Arg Glu Asp Gly Ala Lys Leu Asp Ser Leu 305 310 315 320 Ser Leu Thr Ala Ser Val Gly Asn Ile Ile Ser Ser His Ala Asn Ser                 325 330 335 Ser Ser Ser Ser Ser Ser Ser Gly Ser Ser Ser Ser Ser Ser Ser Ser             340 345 350 Ser Ser Ser Gly Gly Asn Glu Leu Val Ile Ala Ile Asn Ala Gly Gly         355 360 365 Gly Ala Thr Ser Leu Asp Gly Val Asn Phe Val Ala Asp Val His Ser     370 375 380 Leu Gly Gly Ser Thr Gly Ser Thr Thr Asp Ser Ile Ala Gly Ala Thr 385 390 395 400 Ser Ser Thr Leu Tyr Gln Thr Glu Arg Tyr Gly Ser Tyr Ser Tyr Ala                 405 410 415 Val Pro Val Thr Asn Ala Thr Tyr Ser Leu Lys Leu His Phe Ala Glu             420 425 430 Ile Tyr His Thr Glu Ala Gly Ala Arg Ser Phe Asn Leu Ala Val Glu         435 440 445 Gly Gln Gln Glu Met Ala Ser Val Asp Leu Tyr Ser Leu Ser Gly His     450 455 460 Asp Gly Ala Tyr Thr Tyr Glu Val Asn Asp Phe Pro Val Asp Asp Gly 465 470 475 480 Glu Val Thr Ile Ser Leu Glu Ser Leu Thr Asp Asn Gly Thr Leu Ser                 485 490 495 Gly Phe Glu Leu Ser Ser Ser Asp Gly Gly Glu Tyr Val Glu Pro Thr             500 505 510 Pro Ile Pro Thr Pro Glu Pro Gly Glu Gly Gly Arg Tyr Arg Val Ile         515 520 525 His Thr Thr Asp Met Gly Ala Asp Pro Asp Asp Glu Gln Ser Leu Val     530 535 540 Arg Gln Leu Val Met Ala Asn Glu Tyr Asp Leu Glu Gly Ile Ile Thr 545 550 555 560 Thr Thr Gly Cys Trp Lys Lys Ser Thr Ser Asn Thr Ala Tyr Val Asp                 565 570 575 Arg Ile Leu Asn Ala Tyr Ser Gln Ala Tyr Pro Asn Leu Ser Lys His             580 585 590 Ala Glu Gly Phe Pro Thr Pro Ala Tyr Leu Asp Ser Ile Asn Val Met         595 600 605 Gly Gln Arg Gly Tyr Gly Met Gly Asp Val Gly Ser Gly Lys Asp Ser     610 615 620 Ala Gly Ser Asn Leu Ile Ile Ala Ala Val Asp Lys Asp Asp Pro Arg 625 630 635 640 Pro Val Trp Ala Thr Cys Trp Gly Gly Cys Asn Thr Ile Ala Gln Ala                 645 650 655 Val Trp Lys Val Gln Asn Thr Arg Ser Gln Ala Gln Leu Asp Ala Phe             660 665 670 Ile Ser Lys Leu Arg Val Tyr Asp Ile Leu Gly Gln Asp Asn Ala Gly         675 680 685 Thr Trp Leu Ala Lys Asn Phe Pro Asn Leu Ile Tyr Ile Arg Ala Arg     690 695 700 Ser Val Tyr Ser Trp Gln Pro Ser Asp Ser Tyr Leu Asp Asn His Ile 705 710 715 720 Gln Ser His Gly Ala Leu Gly Ala Val Tyr Pro Asn Arg Arg Tyr Ala                 725 730 735 Thr Glu Gly Asp Thr Pro Ala Phe Leu His Met Ala Asn Pro Gly Leu             740 745 750 Asn Asp Pro Ser Val Val Ser Met Gly Gly Trp Gly Gly Arg Phe Pro         755 760 765 Ser Lys Gln Ala Gly Val Arg Gly Met Ser Cys Met Ser Gly Glu Asp     770 775 780 Ala Val Tyr Asp Thr Tyr Tyr Met Tyr Thr Glu Asn Gly Glu Ser Ile 785 790 795 800 Lys Arg Trp Ser Thr Ala Ile His Asn Asp Phe Gln Ala Arg Met Asp                 805 810 815 Trp Ala Ile Glu Ser Asn Tyr Ser Ala Ala Asn His His Pro Val Pro             820 825 830 Val Val Asn Asn Asp Ala Asn Glu Ala Val Met Tyr Leu Asn Ala Ser         835 840 845 Ala Gly Ser Thr Val Ser Leu Asp Ala Ser Gly Ser Ser Asp Pro Asp     850 855 860 Gly Asp Ser Leu Asn Tyr Ser Trp Ser His Tyr Gly Glu Ala Asp Ser 865 870 875 880 Tyr Ser Gly Ser Val Ser Ile Ser Asn Ser Ser Ser Ala Ser Ala Asn                 885 890 895 Val Gln Ile Pro Ser Asn Ala Gly Gly Lys Asp Ile His Ile Leu Leu             900 905 910 Thr Leu Arg Asp Asn Gly Ser Pro Asn Leu Tyr Ala Tyr Arg Arg Val         915 920 925 Val Ile Asn Val Gln     930 <210> 179 <211> 108 <212> PRT <213> Microbulbifer degradans <400> 179 Met Tyr Trp Pro Pro Leu Ala Leu Leu Ser Phe Cys Thr Leu Asn Gly 1 5 10 15 Glu Lys Pro Phe Trp Pro Leu Lys Phe Ser Ser Ser Ile Val Thr Val             20 25 30 Leu Ala Pro Pro Leu Pro Glu Glu Glu Leu Val Leu Glu Leu Asp Glu         35 40 45 Leu Leu Leu Glu Leu Glu Glu Leu Glu Glu Leu Glu Glu Leu Glu Glu     50 55 60 Leu Glu Glu Leu Glu Glu Leu Glu Glu Leu Glu Glu Leu Glu Glu Leu 65 70 75 80 Glu Glu Leu Glu Glu Leu Glu Glu Leu Glu Glu Asp Asp Glu Val Ile                 85 90 95 Pro Pro Ala Gln Glu Gly Asn Ser Lys Leu Pro Leu             100 105 <210> 180 <211> 2802 <212> DNA <213> Microbulbifer degradans <400> 180 atgtatccct tgccatttaa ttcaaagata gttatttcgc ttggtgcaat gacgctcgcg 60 ctggcgacgc agcaagctca ggcgcttagt tgtacggttt cggccgacag ctggaatagc 120 ggttatacgg ccaatgtaac agttgtcaac gatagtagtt acagtattaa tagttgggac 180 gttacgcttg gttttaatca gccgccaagc gtgagcgccg gttggaatgc gaatgtgtct 240 actgttggca ccacagtcat ggccagcaac gtgggttaca atggaaactt gtcgcccggg 300 cagtctacat cctttggttt tcagggggct cacaacggca attttgaatt gccttcttgc 360 gctggaggta tgacttcatc atcctcttct agttcttcta gttcttctag ttcttctagt 420 tcttctagtt cttctagttc ttctagttct tctagttctt ctagttcttc tagttcttct 480 agttcttcta attcttctag ctcgagcagc agctcgtcga gttctagtac cagctcttct 540 tccggcagcg ggggcgctaa tacagtgact attgagctag agaatttgag cggccagaat 600 ggcttttcac catttagcgt gcagaatgac agtagtgcca gtggcggcca gtacatcgtt 660 tggcccaata atggtgacca actgctaagc ggagcctccg atggccaatc tggtactttg 720 gcagtgtcgt ttgaattgtc gcaaacggca aatgtgtcct tcgatataag agcgagcttg 780 gcaaatggca atgatgattc gttttattac aaacttgact ctggtgtttg gagtacccag 840 aacaacactt ctaccagcgg ttttgaaact ctctcgccaa ctacctttaa cggagtgtct 900 gctggtgttc atactcttta tattcagcgc cgagaagatg gagctaagct cgatagtctg 960 agcctgactg cctctgtggg caatattatc agcagccatg ctaatagcag ttctagttct 1020 tcttccagcg gctcaagttc gtctagctct tcttccagtt caagtggtgg taatgagcta 1080 gtaattgcta taaacgctgg gggcggtgcc acatctctag atggtgtgaa ttttgtagct 1140 gatgttcatt cattgggcgg ctccaccggt tccaccaccg acagcatcgc aggcgctact 1200 agcagtactt tgtatcaaac agagcgctac ggcagctaca gctacgctgt tcccgtaact 1260 aatgccactt attcgttaaa gctgcatttt gctgagattt accatactga agcgggggcc 1320 cgctcgttta atcttgctgt ggaaggtcaa caggagatgg ctagtgtaga tctgtattca 1380 ctgtcagggc acgatggtgc ttatacctac gaggtgaatg atttcccggt ggatgatggc 1440 gaggttacga tttcgcttga atcacttacc gataacggca cgcttagtgg tttcgagctt 1500 tcctcctctg acggtggtga atatgtggaa ccaacaccta tacctacgcc agagccgggt 1560 gaaggcggtc gttaccgagt gattcacacc acggatatgg gggcagaccc agacgatgag 1620 cagtcgttgg ttcgccagtt ggtaatggcc aacgagtatg acctagaggg cattattacg 1680 acaacaggtt gttggaaaaa atccaccagt aacacggcct atgttgacag gatcctcaac 1740 gcctacagcc aggcatatcc caatttgagc aagcatgcgg aaggattccc aactccggcg 1800 tatctggatt ccattaatgt gatggggcag cgtggctacg gtatgggtga tgtcggctct 1860 ggcaaagact ctgccgggtc caacctgatt attgctgcag tggataaaga tgatccacgt 1920 cctgtatggg caacttgttg gggtggatgt aatactattg ctcaggcagt gtggaaagtg 1980 caaaacacgc gctcgcaagc gcaattggat gcttttatca gcaagttacg tgtatacgat 2040 attctcgggc aagacaatgc gggcacttgg ctggctaaaa actttccaaa ccttatctac 2100 attcgcgcgc gatctgttta cagctggcaa ccctctgata gttatctgga taatcacatt 2160 cagagtcacg gtgcactggg tgctgtatac ccaaatcgtc gatatgcaac agagggggat 2220 acaccagcat ttctgcatat ggctaatccg ggattgaacg acccatcagt ggtttccatg 2280 gggggctggg gtgggcgttt ccctagcaaa caagctggtg tacgtggtat gtcgtgcatg 2340 agtggtgaag atgccgtcta cgatacctac tacatgtata ctgagaatgg cgaatccatt 2400 aagcggtgga gcactgcaat tcacaacgac tttcaggcac gtatggattg ggccattgag 2460 agtaactatt ctgcagcgaa ccaccaccca gtacccgttg ttaataatga tgctaacgag 2520 gcagtaatgt atctcaatgc gtctgcgggt tcgacagttt cgctggatgc tagcggctcg 2580 agtgacccgg atggagatag ccttaattat tcgtggtctc actacggcga agcggattct 2640 tacagtggtt ccgtgagcat tagtaatagt agttcagcca gcgccaatgt tcagattccg 2700 tcgaatgctg gtgggaagga tatccacatt ttgctaaccc tgcgtgataa cggttcccct 2760 aacctctatg cctaccgccg cgtggttatt aacgtgcaat aa 2802 <210> 181 <211> 584 <212> PRT <213> Microbulbifer degradans <400> 181 Met Arg Cys Val Leu Val Lys Asn Tyr Gln Ile Ile Lys Leu Lys Asn 1 5 10 15 Tyr Ser His His Val Cys Trp Arg Lys Phe Met Ser Thr Gln Gly Lys             20 25 30 Pro Cys Leu Lys Lys Leu Trp Pro Ala Leu Ala Leu Ala Ala Ala Val         35 40 45 Ala Ser Pro Asn Ala Phe Ser Ala Cys Glu Tyr Val Val Ser Asn Gln     50 55 60 Trp Asp Ser Gly Tyr Ser Ala Thr Ile Lys Ile His Asn Asp Thr Asn 65 70 75 80 Ser Thr Ile Asn Gly Trp Asn Val Asn Trp Gln Tyr Ser Gly Asp Asn                 85 90 95 Arg Val Thr Asn Leu Trp Asn Ala Ala Tyr Val Gly Ser Asn Pro Tyr             100 105 110 Ser Ala Ser Asn Leu Ser Trp Asn Ser Thr Val Gly Ala Gly Gln Thr         115 120 125 Ile Glu Phe Gly Phe Gln Gly Ala Lys Asn Gly Gly Ser Ala Glu Val     130 135 140 Pro Val Val Thr Gly Asp Val Cys Ser Gly Gly Gly Ser Ser Ser Gly 145 150 155 160 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Gly Ser Ser Thr Ser                 165 170 175 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Gly Gly Ser Thr Ser             180 185 190 Ser Ser Ser Ser Ser Ser Ser Thr Gly Gly Asn Gly Gly Ala Gln Gln         195 200 205 Cys Asn Trp Tyr Gly Glu Ile Arg Pro Leu Cys Asn Asn Gln Asp Ser     210 215 220 Gly Trp Gly Trp Glu Asn Gln Gln Ser Cys Ile Gly Arg Asp Thr Cys 225 230 235 240 Ser Asn Gln Ser Gly Asn Gly Gly Ile Ile Gly Gly Ser Ser Ser Ser                 245 250 255 Ser Ser Ser Ser Ser Thr Gly Ser Ser Ser Ser Ser Ser Ser Ser Ser             260 265 270 Ser Ser Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser         275 280 285 Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser     290 295 300 Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser 305 310 315 320 Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser                 325 330 335 Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser             340 345 350 Ser Gly Gly Gly Val Ile Phe Ser Ala Asp Phe Glu Ser Asp Ser Asp         355 360 365 Ser Thr Gln Pro Ala Gly Trp Asp Asn Phe Ile Gly Trp Arg Gln Asn     370 375 380 Gly Pro Asn Pro Asn Gly Ser Thr Phe Ala Leu Val Glu Ser Gly Arg 385 390 395 400 Ala His Ser Gly Asn Asn Ala Val His Phe Ser Gly Gly Ala Ser Pro                 405 410 415 Ala Met Ile Ala Arg Ala Leu Pro Ala Asp Leu Asp Thr Leu Tyr Val             420 425 430 Arg Ala Trp Val Tyr Met Thr Arg Gln Leu Gly Met Asn Pro Gly Asp         435 440 445 Asn His Glu Thr Leu Ile Ala Leu Arg Gly Ser Ala Gly Asn Ala Asn     450 455 460 Asn Glu Val Arg Phe Gly Glu Ile Lys Gly Val Ile Gly Thr Asn Glu 465 470 475 480 Val Pro Ser Asp Asn Ile Ala Pro Thr Met Ala Ser Trp Gly Gly Gly                 485 490 495 Pro Ala Tyr Ala Ser Asp Thr Trp His Cys Ile Glu Val Ala Phe Leu             500 505 510 Ser Gln Pro Ala Tyr Asp Thr Val Asn Ala Trp Val Asn Asp Ala Leu         515 520 525 Val His Thr Ile Asp Glu Gly Ser Asp Trp Asn Asn Gly Ala Leu Glu     530 535 540 Ala Asp Trp Leu Ser Asn Lys Tyr Val Glu Leu Ala Phe Gly Trp His 545 550 555 560 Ser Phe Ser Gly Asn Asp Val Asp Val Trp Met Asp Asp Ile Val Val                 565 570 575 Ser Thr Thr Pro Ile Gly Cys Asp             580 <210> 182 <211> 64 <212> PRT <213> Microbulbifer degradans <400> 182 Met Glu Pro Pro Val Glu Leu Leu Glu Glu Leu Leu Glu Glu Leu Leu 1 5 10 15 Val Glu Leu Pro Val Glu Leu Leu Asp Glu Glu Leu Asp Asp Glu Pro             20 25 30 Glu Glu Leu Pro Pro Pro Leu His Thr Ser Pro Val Thr Thr Gly Thr         35 40 45 Ser Ala Leu Pro Pro Phe Leu Ala Pro Trp Lys Pro Asn Ser Ile Val     50 55 60 <210> 183 <211> 1755 <212> DNA <213> Microbulbifer degradans <400> 183 gtgcggtgtg tattagtaaa aaattatcaa ataataaaat taaaaaatta tagccatcat 60 gtttgctgga gaaagtttat gtcaacccaa ggtaaacctt gtttaaaaaa attgtggccg 120 gcgttagcgc ttgctgctgc tgtagctagt ccgaacgcat tttctgcgtg tgagtatgtt 180 gtatccaatc aatgggattc agggtattcg gcgactatta aaattcataa cgatacaaat 240 tcaaccatta atggttggaa tgtgaattgg caatatagcg gcgataaccg cgtaaccaat 300 ttatggaatg cggcgtatgt aggtagtaac ccatactctg caagtaacct ttcgtggaat 360 agcaccgttg gtgccggtca aactatcgag tttggtttcc agggggccaa aaacggcggc 420 agtgccgaag tgcccgttgt tacgggtgat gtgtgtagtg gtggtggcag ttcttctggt 480 tcatcatcta gctcttcatc gagtagctct acaggtagtt ctacaagcag ttcttcaagt 540 agctcttcaa gtagttccac aggcggttcc acaagcagtt catcaagctc gtcttctacc 600 ggtggcaatg gtggtgcaca gcaatgcaat tggtacggtg aaattcgccc actgtgtaac 660 aatcaagata gcggctgggg ttgggaaaat cagcaaagct gtatcggtcg cgacacctgt 720 tccaaccaaa gtggcaatgg cggtataatt ggcggtagtt cttctagctc gtccagttct 780 tcaaccggtt catcgagcag ctcttcgagt tcttcgtcaa gctcatccag ctcttctagt 840 tcaacgtcaa gctcatccag ctcttctagt tcaacgtctt caagttcttc gtcgagttca 900 tcatctagct cttcaagttc tacaagtagc tcgtcgtctt cgtctagctc atcgagtagt 960 tcttcaagca gttcgacgtc tagcagtagt tcttcatctt cgagctctag cagttcatct 1020 agctcttcaa gcagttcttc tagtagctct tcttcaagtg gtggcggtgt tatttttagc 1080 gcagacttcg aaagcgatag cgatagtaca cagccagcag gttgggataa ttttattggc 1140 tggcgtcaaa acgggccaaa cccaaatggt tcgacatttg cgttggtgga atcaggtcgc 1200 gcacacagtg gtaacaacgc tgtgcacttc agtggtggcg caagcccagc tatgattgct 1260 cgcgctttgc ctgcagattt agatacttta tatgtgcgtg cttgggtgta tatgactcgc 1320 caattgggta tgaaccccgg tgataaccat gaaacgctca tcgccttgcg tggctctgct 1380 ggcaatgcaa acaacgaagt gcgttttggt gaaattaaag gggtaattgg tactaacgaa 1440 gtaccgagcg ataacattgc acctactatg gcaagctggg gtggtggccc tgcctatgca 1500 tcagatactt ggcattgtat cgaagtggca tttttatcgc agccagcgta cgacactgta 1560 aatgcatggg ttaacgacgc gcttgtacat actattgatg aaggctctga ctggaacaat 1620 ggtgccttag aggcggattg gttaagtaat aaatacgtag agctagcctt cggctggcac 1680 agctttagcg gcaacgatgt agatgtatgg atggatgata tcgttgtatc cacaacccca 1740 atcggttgcg actaa 1755 <210> 184 <211> 788 <212> PRT <213> Microbulbifer degradans <400> 184 Met Phe Thr Arg Ile Thr Phe Met Leu Ile Asn Lys Ala Arg Gly Arg 1 5 10 15 Leu Ala Ala Ala Met Leu Ala Thr Ala Ala Ser Ala Trp Gly Ser Thr             20 25 30 Ala Leu Ala Glu Cys Ser Tyr Gln Val Thr Asn Asn Trp Gly Ser Gly         35 40 45 Phe Thr Ala Ala Ile Arg Ile Thr Asn Thr Gln Thr Ser Ser Ile Asn     50 55 60 Asp Trp Gln Val Ser Trp Thr Tyr Glu Asn Asn Thr Leu Val Asn Ala 65 70 75 80 Trp Asn Ala Asn Val Ser Gly Asn Tyr Thr Ala Thr Asn Met Gly Trp                 85 90 95 Asn Gly Ser Leu Thr Ser Gly Gln Ser Val Glu Phe Gly Leu Gln Gly             100 105 110 Thr Thr Thr Gly Gly Val Val Glu Ile Pro Leu Leu Thr Gly Asn Val         115 120 125 Cys Asn Ser Asn Thr Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ala     130 135 140 Ser Ser Ser Ser Ser Ser Ser Gly Ala Ala Ala Val Asn Leu Ala Gly 145 150 155 160 Ile Ala Asn Ala Ser Thr Ser Tyr Val Ser Ala Trp Glu Thr Leu Ala                 165 170 175 Ala Val Asn Asp Gly Asn Thr Pro Ala Asn Ser Asn Asp Lys Ser Asn             180 185 190 Gly Ala Tyr Gly Asn Trp Asn Asn Pro Asn Ser Ile Gln Trp Val Gln         195 200 205 Tyr Asp Trp Pro Gln Asn Tyr Thr Leu Ser Ser Thr Gln Ile Tyr Trp     210 215 220 Phe Asp Asp Asn Gly Gly Val Leu Val Pro Asp Val Ala Tyr Ile Glu 225 230 235 240 Tyr Trp Ser Asn Gly Thr Trp Val Lys Val Gly Asp Val Pro Arg Gln                 245 250 255 Glu Asn Thr Phe Asn Thr Leu Asn Leu Asn Asn Ile Val Thr Asn Arg             260 265 270 Leu Arg Val Ser Ile Ser Asn Thr Leu Gln Ser Thr Gly Ile Leu Glu         275 280 285 Trp Arg Val Glu Gly Thr Glu Pro Ser Gly Ser Ser Ser Ser Ser Ser     290 295 300 Ser Ser Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Gly Gly Pro Glu 305 310 315 320 Gln Cys Asp Ala Tyr Val Trp Pro Glu Tyr Glu Pro Asn Leu Asn Tyr                 325 330 335 Asp Phe Arg Gln Asp Tyr Ala Asp Ile Asn Pro Glu Glu Phe Glu Val             340 345 350 Phe Leu Gly Cys Asp Pro Ser Gln Val Ala Gly Val Lys Thr Ser Gly         355 360 365 Trp Tyr Ala Phe Ile Trp Gly His Asn Arg Asn Pro Ala Ile Thr Asp     370 375 380 Glu Asp Ile Asp Arg Val Leu Ala Asn Leu Asn Glu Asp Met Ala Tyr 385 390 395 400 Ala Arg Gly Glu Met Gly Trp Pro Pro Asp Lys Leu Pro Gln Glu Gly                 405 410 415 Tyr Tyr Ser Asn Val Tyr Leu Tyr Gly Ser Gly Leu Cys Thr Asp Asn             420 425 430 Ala Ala Asn Thr Glu Arg Gly Gly Trp Gln Ser Ser Ile Ala Gly Tyr         435 440 445 Pro Met Val Leu Leu Ser Tyr Tyr Pro Val Ile Thr Pro Ser Glu Arg     450 455 460 Gly Gly Ile Thr His Glu Ala Ile His Thr Ile Met Ala Ser Met Gly 465 470 475 480 Asn Lys Ala Ala Trp Phe Asn Glu Gly Gly Asn Thr Trp Leu Gln Met                 485 490 495 Asn Met Glu Ala Ser Arg Val Gly Asp Tyr Gly Val Gly Phe Leu Asp             500 505 510 Gly Ala Pro Phe Leu Ala Pro His Met Pro Ile Glu Asn Tyr Ser Gly         515 520 525 Trp Leu Gln Asp Gly Ser Phe Gly Gly Pro Asn Ala Glu Gly Val His     530 535 540 Arg Glu Leu Asn Gly Gln Gln Ile Ala Thr Trp Arg Asp Tyr Leu Gly 545 550 555 560 Gly His Gln Tyr Asn Ser Val Phe Ser His Phe Leu Ala Gln Tyr Val                 565 570 575 Ser Ser Gly Ala Asn Ala Trp Ile Trp Lys Asn Gly Pro Tyr Asn His             580 585 590 Ile Leu Ala Ser Leu Ala Ala Gly Leu Gly Asp Asp Gln Thr Arg His         595 600 605 Leu Ile Met Gln Tyr Arg Ala Arg Gln Ala Met Val Asp Phe Gly Pro     610 615 620 Trp Thr Asn Gly Phe Lys Gln Pro Ile Asn Asn Asn Trp Gln Arg Thr 625 630 635 640 Ile Gly Ala Glu Glu Thr Ala Ala Gly Lys Trp Met Glu Pro Glu Pro                 645 650 655 His Gln Leu Thr Phe Tyr Ala Ala Thr Ser Gln Glu Gly Asn Thr Leu             660 665 670 Ile Pro Ala Gln Asn Thr Leu Pro Gly Trp Ser Gly Ala Asn Gln Ile         675 680 685 Pro Leu Gln Val Thr Gly Asn Lys Val Arg Val Asp Phe Glu Pro Phe     690 695 700 Gly Asn Asn Met Arg Leu Gln Leu Ala Tyr Arg Ala Gln Asp Gly Ser 705 710 715 720 Ala Val Tyr Ser Gln Pro Ile Glu Ser Gly Glu Ala Cys Leu Thr Leu                 725 730 735 Glu Lys Thr Pro Lys Asn Gly Val Val Val Ala Ile Val Ser Asn Thr             740 745 750 Asp Tyr Thr Tyr Ala Gly Asp Glu Thr Arg Lys Gln Lys Tyr Asp Tyr         755 760 765 Arg Val His Ile Gln Glu Gly Val Ser Gly Thr Ala Ser Leu Tyr Ser     770 775 780 Lys His Tyr Glu 785 <210> 185 <211> 2367 <212> DNA <213> Microbulbifer degradans <400> 185 atgtttacga ggattacctt tatgttaatt aacaaagcga gaggtcgctt agctgcagca 60 atgttagcca cagcagcctc agcttggggt agcacggctc tagccgagtg cagctatcaa 120 gtcacaaata attggggcag cggatttact gccgccattc gcatcactaa tacccaaacg 180 agtagtatta atgactggca agttagctgg acgtacgaaa ataatacact tgtaaatgct 240 tggaacgcga atgttagcgg caattacacc gctacaaaca tgggctggaa tggctcactt 300 acctcgggcc aatctgtcga gtttggcctg caaggcacca caactggcgg tgtggttgaa 360 atcccccttt taaccggtaa tgtgtgcaac tcaaatacaa gtagtagctc ttctagtagt 420 acaagttcag cctcaagttc aagttcgagt tccggcgctg ccgcagtaaa tctcgctggt 480 atagccaatg cgagcacttc ctatgtttct gcatgggaaa cattggcagc agtgaatgat 540 ggtaacaccc cggccaattc caacgacaag tccaacggag cttacggcaa ctggaacaat 600 ccaaattcga tacagtgggt acagtacgat tggccacaaa actatacgtt atcctcaacg 660 caaatatatt ggtttgatga taatggcggc gtactggtac ccgatgttgc ttacattgaa 720 tattggagca atggcacatg ggtaaaagta ggtgatgtac cgcggcaaga aaatacattt 780 aacacgctaa atttaaacaa tattgttaca aaccgcctac gtgtttctat aagcaacaca 840 ttgcaatcta caggcatact ggagtggcgc gtagagggaa cagagccaag cggcagctca 900 tctagcagca gctcaagctc atcatccagc tcaacgtcta gtagtagtgg cggaccagaa 960 cagtgtgatg cgtatgtgtg gcccgaatac gagccgaatt taaattacga ctttagacag 1020 gactacgcag atataaaccc cgaagaattt gaggtatttt taggttgcga cccaagccaa 1080 gttgcaggtg ttaaaacttc tggctggtac gcgtttattt gggggcataa tcgcaacccc 1140 gcaattaccg atgaagatat agatagagta ttggccaatt taaatgaaga tatggcatac 1200 gctcgcggcg aaatgggctg gccacccgat aagcttcctc aggaaggcta ctacagcaat 1260 gtgtatttgt acggctctgg tttatgtacc gataacgcag ccaataccga gcgaggtggc 1320 tggcaaagta gcattgccgg ctaccccatg gtactgcttt cgtattaccc agtaataaca 1380 cctagcgagc gcggtggtat aacccacgaa gctatccaca ctattatggc ttcgatggga 1440 aacaaagctg catggtttaa cgaaggcggc aacacttggc tacaaatgaa tatggaggca 1500 tctcgcgttg gggattatgg cgttggcttt ttagatggtg cccccttttt agcgccgcac 1560 atgccaatcg aaaattacag tggttggcta caagatggct ccttcggtgg gccgaatgct 1620 gaaggcgtac accgggaatt aaatggtcag caaatagcaa cttggcgaga ttacttaggc 1680 ggccatcaat acaactctgt gttttctcac tttttagcgc aatatgtttc tagcggtgca 1740 aatgcgtgga tatggaaaaa cgggccatat aaccatattc tcgcctcgct agcagcgggc 1800 ttgggcgacg accaaactcg ccacttaatt atgcaatacc gcgcacggca agcaatggtt 1860 gattttggcc cttggacgaa tggatttaaa caaccaatca ataacaattg gcaacgcacc 1920 attggtgcag aagaaaccgc tgctggcaaa tggatggagc ctgaacccca tcaattaacg 1980 ttttatgctg caacaagcca ggaaggtaat actttgatac cagcacaaaa tacattgccg 2040 ggctggtctg gtgccaatca aataccatta caagtaaccg gcaacaaagt gcgtgttgat 2100 tttgaaccat tcggcaacaa tatgcgcttg caattagcct atcgcgcaca agacggttca 2160 gcggtataca gccagcctat agaaagtggc gaagcgtgct taacactaga aaaaacaccc 2220 aaaaatggcg tagtggtggc aattgtatcc aacaccgatt acacctacgc aggggatgaa 2280 actcgcaaac aaaaatacga ttacagagtg catattcaag aaggcgtaag cggtacggca 2340 tcactttata gcaagcacta tgaatag 2367 <210> 186 <211> 787 <212> PRT <213> Microbulbifer degradans <400> 186 Met Leu Arg Lys Leu Asn Phe Ile Leu Ala Pro Leu Gly Met Val Leu 1 5 10 15 Gly Val Asn Thr Tyr Ala Asp Val Ser Cys Ala Val Ser Gly Ser Val             20 25 30 Trp Asn Asn Gly Tyr Val Ala Asn Val Ala Val Thr Asn Ala Gly Glu         35 40 45 Ser Leu Gln Glu Gly Trp Ser Val Ala Leu Leu Phe Asp Asn Thr Pro     50 55 60 Thr Ile Asn Asn Ser Trp Ser Ala Glu Leu Ala Val Asp Gly Asn Val 65 70 75 80 Leu Thr Ala Ser Asn Val Ala Trp Asn Ala Asn Leu Ala Ala Gly Gln                 85 90 95 Ser Ala His Phe Gly Phe Val Gly Ser Tyr Ser Gly Glu Phe Glu Leu             100 105 110 Pro Glu Cys Phe Val Gly Ala Leu Asp Asp Asp Thr Ala Leu Glu Asp         115 120 125 Tyr Leu Lys Gln Gly Val His Asn His Leu Asn Leu Arg Ile Ala Asn     130 135 140 Ser Ala Ser Gly Ser Ser Ser Ser Ser Ser Ser Ser Gly Ala Ser Ser 145 150 155 160 Ser Ser Ser Ser Ser Ser Ala Ser Ser Ala Ser Ser Thr Ser Ser Gly                 165 170 175 Ser Pro Val Ala Glu Pro Asp Thr Gln Pro Asp Asp Ile Thr Asn Thr             180 185 190 Gln Glu Asp Gly Val Asp Glu Ala Asp Ile Ile Glu Ser Asp Gly Asn         195 200 205 His Phe Phe Val Val Arg Ser Ser Leu Tyr Phe Thr Gly Thr Ser Ser     210 215 220 Ser Thr Ser Gly Ser Ser Ser Ser Ser Ala Ser Ser Ser Gly Ser Gly 225 230 235 240 Gly Tyr Gly Asp Tyr Thr Gln Gly Val Leu Ile Glu Ala Tyr Ser Lys                 245 250 255 Asp Ser Ala Ala Gly Thr Thr Gln His Val Gly Gln Val Thr Leu Pro             260 265 270 Tyr Glu Glu Asn Tyr Leu His Val Ser Gly Ala Tyr Phe Arg Lys Thr         275 280 285 Pro Gln Gly Asn Arg Leu Ala Ile Val Ser Asn Thr Arg Glu Ser Gln     290 295 300 Pro Tyr Tyr Trp Ser Tyr Gly Tyr Gly Tyr Tyr Pro Tyr Tyr Gln Ile 305 310 315 320 Ser Asn Lys Val Ala Ile Ser Val Ala Asn Ile Asp Thr Pro Glu Leu                 325 330 335 Met Asp Glu Ala Glu Arg Ile Thr Phe Asp Gly Asp Leu Val Ser Ser             340 345 350 Arg Arg Ile Gly Ser Lys Leu Tyr Ile Ala Ser Arg Tyr Ser Pro Asn         355 360 365 Leu Asn Arg Leu Gly Phe Asp Phe Ser Ser Ser Ala Ser Asn Glu Glu     370 375 380 Asn Ala Gln Arg Leu Asp Glu Ala Pro Leu Ala Asp Leu Leu Pro His 385 390 395 400 Val Tyr Asp Glu Asn Gly Val Gly Thr Pro Leu Val Thr Ala Ala Asp                 405 410 415 Cys Thr Val Pro Glu Trp Pro Glu Ser Val Asp Val Tyr Ala Gly Ser             420 425 430 Leu Leu Val Ile Thr Gln Ile Asp Leu Asp Asn Asn Leu Ala Ile Ser         435 440 445 Ser Arg Cys Leu Pro Ala Ser Ala Thr Glu Ile Tyr Ser Ser Ala Asp     450 455 460 Ala Ile Tyr Ala Phe Ser Asn Ala Phe Tyr Ala Gly Val Lys Val His 465 470 475 480 Lys Phe Thr Leu Lys Asn Asp Ala Gln Glu Ser Glu Ile Ser Tyr Lys                 485 490 495 Gly Ser Val Lys Leu Pro Gly Phe Ile Ala Cys Ser Asn Gln Ser Tyr             500 505 510 Cys Phe Gly Glu Gln Asp Gly Ala Leu Arg Val Leu Tyr Arg Val Ser         515 520 525 Asn Tyr Ser Asp Ala Thr Pro Tyr Arg Ile Ala Val Ile Lys Gln Ser     530 535 540 Pro Ser Ser Ala Glu Gly Leu Asp Ile Ile Ala Thr Leu Pro Asn Ala 545 550 555 560 Glu Arg Pro Ala Ser Ile Gly Lys Pro Gly Glu Ile Val Tyr Ala Met                 565 570 575 Arg Ser Phe Gly Asp His Ala Tyr Val Val Thr Phe Asp Arg Ile Asp             580 585 590 Pro Leu Tyr Ala Ile Asp Phe Ser Asn Pro Ala Asp Pro Tyr Ile Ala         595 600 605 Gly Glu Leu Glu Val Thr Gly Val Ser Asp Tyr Leu His Pro Val Gly     610 615 620 Glu Asn Leu Leu Leu Gly Val Gly Arg Asp Ala Ile Phe Asp Glu Ala 625 630 635 640 Arg Asn Leu Thr Trp Phe Gln Gly Val Lys Val Glu Leu Phe Asp Val                 645 650 655 Ser Asp Pro Thr Asn Leu Arg Ser Leu Gly Ser Glu Val Ile Gly Lys             660 665 670 Arg Glu Ser Ser Thr Thr Leu Ser Phe Asp Ala Arg Gly Ile Ala Phe         675 680 685 Ser His Asp Glu Asn Gly Ile Arg Phe Ala Ile Pro Val Lys Met His     690 695 700 Asp Lys Pro Ile Gly Pro Ala Asn Thr Asp Ala Leu Asn Gln Tyr Tyr 705 710 715 720 Ser Trp Thr His Thr Gly Leu Tyr Val Tyr Gln Ile Glu Thr Gln Pro                 725 730 735 Asn Thr Asp Ala Ser Leu Ser Arg Leu Gly Ile Leu Lys Thr Asp Ile             740 745 750 Gly Glu Ala Ser Val Arg Tyr Asp Arg Gly Val Ile Asp Gly Asp Ala         755 760 765 Val Tyr Tyr Leu His Asn Tyr His Met Phe Ser Ser Leu Ile Asn Tyr     770 775 780 Leu Ala Gln 785 <210> 187 <211> 2364 <212> DNA <213> Microbulbifer degradans <400> 187 atgttacgga aattaaattt tattttagcg ccgcttggta tggtgttagg cgttaacacc 60 tatgcggatg tgtcgtgcgc tgtttctggc agcgtgtgga acaatggcta tgtagctaat 120 gttgcagtta ctaacgcggg tgaatctctg caagagggtt ggagtgttgc tttgttgttc 180 gataacactc caactataaa caatagttgg agtgcggagc ttgctgtaga cggtaatgtg 240 ctaaccgcga gtaatgtggc ttggaacgct aatttggcag cggggcaatc tgctcatttt 300 ggttttgttg gctcatattc aggtgagttt gaattgcctg aatgtttcgt tggcgcacta 360 gatgatgaca ctgcactcga agattattta aaacagggcg tgcataatca tttaaattta 420 cgcattgcaa attcggcgtc tggttcatct agctcttcga gctcttccgg tgcgtccagt 480 tcttccagtt catccagtgc ttctagcgca tcgagtacat cttctggttc gcccgtggcc 540 gagcccgata ctcagcctga tgatattacc aatacccaag aagatggcgt ggatgaggcg 600 gatataattg aatccgacgg taaccacttc tttgtggtgc gttcttcttt atattttacc 660 ggcactagct catcaacctc tggctctagt tctagttcgg cttcatctag cggttcaggt 720 gggtatggcg attacacaca gggagtgttg attgaagcct acagtaaaga ttcagcagcc 780 ggaacaactc aacatgtggg gcaggtgacg ttgccctatg aagaaaatta tcttcacgtt 840 tctggtgctt actttcgcaa aacaccgcaa ggcaatagat tagctattgt aagtaacacc 900 agagaatcac agccttatta ttggagctat gggtatggtt attaccccta ttatcaaatt 960 tctaataagg tagctatttc tgttgctaat attgataccc ctgaattaat ggacgaggca 1020 gagcgaatta cttttgatgg tgatttagta tcgagcagac gtattggtag caaattatat 1080 attgccagcc gttatagccc gaacctaaat cgtttagggt ttgatttttc tagcagcgcg 1140 agcaatgagg aaaatgcaca gcgtttagat gaagcgcccc ttgcggattt attgccacat 1200 gtttacgacg aaaatggcgt aggtacgcct ttggttactg ctgctgactg caccgtaccg 1260 gagtggccag aaagcgtgga tgtatatgcc gggtcactgt tggttattac tcaaattgac 1320 ttggataata acttagcaat tagctcgcgt tgtttacctg cttcggcaac agaaatatac 1380 agttctgcgg atgcaattta tgctttctcc aatgcatttt atgctggggt aaaagtgcat 1440 aaattcacat taaaaaatga tgcccaagaa agtgaaatta gctataaggg tagtgttaag 1500 ttgccgggtt ttatagcttg cagtaatcaa tcttattgct ttggcgagca agacggggca 1560 ttgcgcgttt tgtatagagt gtccaattac agtgatgcta ctccctatcg tattgcggta 1620 attaagcaat cgccaagctc tgcagaaggt ttagacatta tagctacctt gccaaatgct 1680 gaacgcccag cctctattgg taagccgggc gagattgttt atgccatgcg tagttttggt 1740 gatcacgctt acgtcgttac tttcgataga attgacccgt tatatgctat tgatttctct 1800 aacccagcag acccttacat tgccggtgag cttgaggtta ctggtgtgtc tgattactta 1860 catccagtgg gggagaattt attgttgggc gtgggccgag atgcaatatt cgacgaagcg 1920 cgcaatttaa cttggtttca gggtgttaaa gtggagctgt ttgatgtgag tgaccctaca 1980 aacctgcgca gcttaggcag tgaagtaata ggtaagcgcg agtcgtcaac gacacttagt 2040 tttgatgccc gtggtattgc atttagccat gatgaaaacg gtattcgttt tgctattcct 2100 gtgaaaatgc atgataagcc aattggaccg gcaaataccg atgcgttaaa ccaatattat 2160 agttggaccc atacggggtt gtatgtttac caaatagaaa cgcagccaaa caccgatgct 2220 agtttgtctc gcttaggtat actaaaaact gatatcggcg aggccagtgt gcgctatgat 2280 cgtggcgtaa ttgatggtga tgctgtttac tatttgcata attaccacat gttttcgagc 2340 ttaatcaatt atttggcgca atag 2364 <210> 188 <211> 681 <212> PRT <213> Microbulbifer degradans <400> 188 Met Arg Ile Ser Thr Ala Ile Thr Gln Phe Leu Ile Val Ile Ala Thr 1 5 10 15 Leu Ile Leu Ala Ala Cys Ser Gly Ser Gly Gly Gly Ala Asn Ser Gly             20 25 30 Gly Ser Asn Pro Gly Ala Ser Ser Ser Ser Ser Ser Ser Ser Ser         35 40 45 Ser Asn Ser Ser Ser Ser Ser Thr Ser Ser Ser Ser Ser Ser Gly Gly     50 55 60 Ser Ser Gly Ala Ala Val Ser Leu Pro Ser Thr Ile Ala Ala Met Asp 65 70 75 80 Phe Val Ala Ala Tyr Asp Thr Asp Asn Ala Asn Ser Gly Asp Cys Gly                 85 90 95 Asn Gly Pro Val Asp Met Gln Thr Ser Thr Asp Thr Gln Gly Ala Ala             100 105 110 Cys Thr Val Gly Trp Thr Lys Ala Gly Glu Trp Leu Ala Tyr Asp Val         115 120 125 Ser Val Ala Val Thr Gln Lys Met Asp Ile Val Phe Arg Val Ala Thr     130 135 140 Asn Gln Asn Ser Arg Ala Leu Lys Val Gln Leu Asn Asn Lys Thr Leu 145 150 155 160 Gly Val Leu Asn Val Ser Gly Thr Ala Phe Asp Glu Trp Gln Thr Val                 165 170 175 Ser Leu Lys Asp Ile Glu Ile Pro Ala Gly Thr His Gln Leu Lys Leu             180 185 190 Val Trp Met Thr Gly Ala Ile Asn Val Asn Thr Leu Ser Phe Thr Ala         195 200 205 His Ser Ala Tyr Gly Gln Thr Thr Asn Leu Trp Gly Ser Asn Gly Ala     210 215 220 Glu His Asp Pro Ser Gly Val Leu Ser Asp Trp Ser Tyr Ala Gly Tyr 225 230 235 240 His Trp Gly Glu Glu Glu Pro Pro Val Lys Ser Pro Thr Ile Asn Val                 245 250 255 Val Thr Asp His Gly Ala Ile Ala Asp Asp Asp Ser Asp Asp Ser Ala             260 265 270 Ala Phe Ile Ala Ala Leu Thr Ala Ala Asn Asn Gly Asp Val Val Tyr         275 280 285 Val Pro Glu Gly Arg Phe Ile Leu Thr Gln Val Leu Ser Ile Pro Asn     290 295 300 Gly Val Val Leu Gln Gly Ala Gly Ser Glu Leu Thr Thr Leu Tyr Ile 305 310 315 320 Pro Thr Asn Leu Asn Glu Ala Thr Gly Ile Asp Pro Ser Phe Thr Gly                 325 330 335 Gly Phe Ile Glu Met Lys Gly Ser Ser Ser Asp Gly Ser Lys Leu Ser             340 345 350 Thr Ile Thr Ala Ala Ala Ala Arg Gly Ser Asn Gln Ile Thr Val Glu         355 360 365 Ser Val Ala Gly Leu Thr Val Gly Asp Trp Val Gln Ile Gln Gln Thr     370 375 380 Asp Val Asn Gly Asn Phe Leu Leu Glu Ala Leu Tyr Ala Gly Tyr Thr 385 390 395 400 Ala Gly Leu Ser Asp Tyr Ser Asn Leu Val Asn Asp Ala Glu Leu Glu                 405 410 415 Phe Phe Ser Arg Ile Thr Asn Ile Ser Gly Asn Thr Leu Thr Leu Glu             420 425 430 Arg Pro Leu Pro Ile Thr Val Ser Pro Asn Tyr Met Ala Glu Leu His         435 440 445 Ser Val Ser Val Ala Tyr Gly Glu Val Gly Phe Glu Gly Met Thr Leu     450 455 460 Glu Phe Pro Glu Thr Thr Tyr Pro Gly His Phe Asn Glu Leu Gly Tyr 465 470 475 480 Asn Gly Ile Asp Met Arg Ala Gln His Ser Trp Val Arg Asp Val Val                 485 490 495 Val Lys Asn Ile Asp Tyr Gly Ile Asn Leu Arg Gly Ser His Phe Val             500 505 510 Ser Ile Leu Asp Phe Thr Val Ile Asn Thr Asn Asp Arg Ser Gly His         515 520 525 His Gly Ile Ser Val Gly Ser Ser Thr Asp Cys Leu Ile Arg Gly Phe     530 535 540 Asp Ile Gln Ala Glu Leu Val His Asp Leu Thr Val Glu Trp Tyr Ala 545 550 555 560 Tyr Gly Asn Val Phe Thr Gln Gly Arg Gly Lys Asn Ile Thr Leu Asp                 565 570 575 His His Arg Ala Ala Ala Tyr Ala Asn Leu Phe Thr Gln Ile Asp Leu             580 585 590 Gly Glu Ala Thr Arg Ala Trp Lys Thr Gly Gly Arg Ser Asp Arg Gly         595 600 605 Tyr Lys Thr Ala Val Tyr Ser Thr Phe Trp Asn Met Thr Ala Glu Gln     610 615 620 Ala Ile Asp Trp Pro Ala Asn Asp Phe Gly Pro Arg Met Val Phe Met 625 630 635 640 Gly Leu Thr Met Asp Gly Ser His Ser Ser Val Leu Asp Trp Val Val                 645 650 655 Glu Asp Ile Ser Ala Asp Asp Ile Tyr Pro Pro Asn Leu Trp Leu Ser             660 665 670 Gln Arg Glu Lys Arg Leu Gly Arg Gln         675 680 <210> 189 <211> 66 <212> PRT <213> Microbulbifer degradans <400> 189 Met Leu Gly Ser Asp Thr Ala Ala Pro Asp Glu Pro Pro Leu Glu Asp 1 5 10 15 Asp Glu Leu Val Leu Leu Glu Leu Leu Leu Asp Glu Glu Asp Glu Glu             20 25 30 Leu Glu Leu Glu Leu Ala Pro Gly Leu Glu Pro Pro Glu Phe Ala Pro         35 40 45 Pro Pro Glu Pro Leu Gln Ala Ala Arg Ile Ser Val Ala Ile Thr Ile     50 55 60 Arg asn 65 <210> 190 <211> 2046 <212> DNA <213> Microbulbifer degradans <400> 190 atgcgtattt ctactgctat cactcaattc ctaattgtaa ttgctacact tattctagct 60 gcctgcagtg gctcgggcgg aggggcgaat tctggtggct ctaatcctgg tgctagttct 120 agctccagtt cttcgtcttc ttcatctaat agcagttcga gtagcactag ttcgtcgtcc 180 tctagtggtg gttcatccgg tgccgcggta tcgctcccaa gcactattgc cgcaatggat 240 tttgtcgcag cttacgatac agacaacgca aattcaggtg attgtggtaa tggccctgtc 300 gatatgcaaa cgtcaaccga tacgcaaggt gcggcctgca cggtaggttg gacaaaagct 360 ggcgagtggt tggcttatga tgtgagtgtt gctgttaccc aaaaaatgga tattgtgttt 420 cgtgttgcta ctaaccaaaa ctcacgtgcc ttaaaagtac agctaaataa caaaacattg 480 ggtgtgttaa atgtttctgg tacagcgttt gatgaatggc aaactgtatc gctaaaagac 540 atagaaattc ctgcaggcac gcatcaatta aaactcgtgt ggatgaccgg ggcaataaac 600 gtcaataccc ttagttttac cgcgcacagc gcctacggcc aaactaccaa tttgtggggc 660 agtaacggtg ccgaacacga cccgagcggc gtgttatctg attggtctta tgctggttat 720 cactggggtg aagaagagcc gccggtaaaa tcgccaacca ttaatgtggt taccgatcac 780 ggtgcgatag ccgatgacga tagcgacgat agcgctgcgt ttattgctgc attaaccgca 840 gcaaataatg gtgatgtagt ttacgtaccc gaggggcgtt ttattcttac gcaggtgcta 900 agcataccga atggtgttgt gttgcagggc gcaggcagcg aattaactac tttatatatt 960 cccaccaact taaatgaagc aacgggtata gatccatctt ttactggtgg gtttattgaa 1020 atgaaaggtt cgtcgagtga cggcagtaaa ctttctacta ttactgcagc ggcggcacga 1080 ggcagtaatc aaataacagt agaaagtgtt gcaggtttaa ccgtaggcga ttgggtgcaa 1140 atacaacaaa ccgatgtgaa cggtaacttt ttattagagg ccttgtatgc agggtacacc 1200 gcaggcttaa gtgattacag taatttggtt aacgatgcag agttagagtt tttcagccgc 1260 ataacaaata ttagtggcaa cacacttacc ctagagcgac cgctacccat taccgtaagc 1320 cctaactata tggcggaatt acacagcgta tcggttgcct acggcgaggt gggtttcgaa 1380 ggaatgactt tagagtttcc agaaacaacc tacccaggtc acttcaatga gttagggtac 1440 aacggcatag atatgcgcgc tcagcactct tgggtgcgag atgttgtagt aaaaaatata 1500 gattatggta ttaatttaag aggctcgcac tttgtaagca ttttagattt tactgtaatt 1560 aacacaaatg atcgcagtgg ccaccacggg ataagtgtgg ggtcgtccac cgattgttta 1620 attcgcggtt tcgatattca agccgaacta gtccacgatt taactgttga atggtatgcg 1680 tatggcaatg tatttacgca agggcgaggc aaaaatatta ccctcgatca tcaccgcgca 1740 gcagcctacg ccaacttatt tacccaaatt gatttaggtg aagcaacacg cgcatggaaa 1800 acaggtggcc gaagcgatcg cggatacaaa accgcggtat acagtacgtt ttggaacatg 1860 accgccgaac aggcgatcga ttggcccgcc aatgatttcg gcccacgtat ggtgtttatg 1920 gggttaacca tggacggcag tcacagctct gttttagatt gggtggtaga agatatttct 1980 gcagatgata tatatccacc aaacctttgg ttgtcgcaaa gagaaaaaag attggggcgg 2040 cagtag 2046 <210> 191 <211> 654 <212> PRT <213> Microbulbifer degradans <400> 191 Met Arg Phe Gln Tyr Ala Leu Cys Thr Asp Ser Gly Gly Thr Ile Lys 1 5 10 15 Asn Asn Gln Ser Trp Val Ser Ile Met Leu Arg Ile His Leu Ala Met             20 25 30 Pro Asn Met Ala Phe Ser Leu Leu Gln His Tyr Leu Leu Asn Thr Phe         35 40 45 Ser Gly Val Ser Ile Trp Val Leu Cys Gly Leu Leu Met Gly Ala Tyr     50 55 60 Val Asn Ala Ala Thr Gln Glu Ile Asn Gly Asn Gln Leu Gln Val Thr 65 70 75 80 Cys Gln Ser Glu Thr Phe Cys Asp Ile His Tyr Arg Leu Asn Asn Gly                 85 90 95 Arg Glu Leu Asn Ile Ala Met Ala Ser Leu Gly Asn Gly Glu Tyr Ala             100 105 110 His Thr Ile Ser Asn Leu Ile Ser Gly Asp Val Ile Val Tyr Tyr Leu         115 120 125 Thr Tyr Gln Gln Asn Gly Leu Ala Tyr Asp Ser Glu Arg Leu Thr His     130 135 140 Ile Tyr Gly Gly Ala Ser Ser Ser Ala Gly Ala Asp Pro Tyr Leu Gly 145 150 155 160 Phe Arg Ala Pro Ile Pro Gly Thr Ile Leu Ala Val Asn Tyr Asp Thr                 165 170 175 Gly Gly Glu Gly Ile Ala Phe His Asp Ala Thr Ile Gly Asn Ser Gly             180 185 190 Gly Gln Tyr Arg Asp Glu Ser Val Asp Ile Glu Ser Ala Ser Ile Gly         195 200 205 Gly Phe Asn Val Gly Trp Val Ala Ser Gly Glu Trp Leu Gln Tyr Ser     210 215 220 Val Asp Val Ala Arg Ala Gly Ser Phe Asp Ile Val Ala Gln Ile Ala 225 230 235 240 Ser Pro Asn Ser Gly Gly Ser Leu His Tyr Glu Ile Thr Gly Ala Thr                 245 250 255 Asn Val Gln Ser Asp Ala Val Gln Phe Ala Asn Thr Gly Gly Trp Gln             260 265 270 Val Trp Ala His Thr Ala Ala Ala Lys Val Gln Leu Asn Ala Gly Ala         275 280 285 His Thr Ile Arg Leu Val Phe Glu Ser Ala Gly Phe Asn Ile His Ser     290 295 300 Leu Leu Val Ser Glu Ala Ser Ser Ser Phe Gly Lys Val Glu Ala Glu 305 310 315 320 Ser Phe Asp Ser Met Ser Gly Val Val Ser Glu Ala Thr Ser Val Gly                 325 330 335 Tyr Phe Asp Arg Gly Asp Trp Met Lys Tyr Ser Ala Val Ser Phe Gly             340 345 350 Asn Leu Ala Lys Ser Ile Thr Leu Ser Val Ala Gly Glu Tyr Asn Asn         355 360 365 Gly Ile Ala Glu Leu Arg Leu Asp Ser Val Ser Gly Pro Val Ile Gly     370 375 380 Thr Tyr Ser Met Glu Ser Thr Gly Gly Trp Ala Ser Phe Lys Pro Lys 385 390 395 400 Ser Phe Asn Ile Val His Thr Leu Gly Val His Asp Leu Tyr Ile Val                 405 410 415 Gly Lys Asn Gly Ser Gly Ile Phe Asn Leu Asp Tyr Phe Gln Leu Ser             420 425 430 Ala Asp Val Val Glu Glu Thr Asn Pro Ala Thr Ala Val Lys Ala Met         435 440 445 Ser Leu Asn Ile Tyr Gly Trp Ala Thr Met Pro Gln Asn Ala Asp Lys     450 455 460 Tyr Ala Ala Leu Ile Arg Ser Arg Gly Val Asp Val Val Gly Ile Gln 465 470 475 480 Glu Gly Val Glu Asp Trp Leu Ile Gly Pro Gly Phe Pro Thr Asn Tyr                 485 490 495 Ser Lys Ala Asp Ala Leu Gly Ala Ala Leu Gly Ala Cys Trp Gln Gln             500 505 510 Arg Tyr Gln Ile Phe Ile Asn Ile Cys Glu Gly Asn Ser Phe Val Ser         515 520 525 Asn Arg Arg Phe Asp Met Thr Asp Gly Pro Asn Ala Thr Arg Thr Gly     530 535 540 Glu Ser Ala Arg Ile Asn Lys Asn Gly Phe Glu Tyr Ala Val Leu Thr 545 550 555 560 Val His Trp Asp His Gln Ser Gly Ala Ala Lys Val Ala Asn Ala His                 565 570 575 Glu Thr Ala Ala Glu Val Asn Tyr Tyr Gly Ala Leu Pro Thr Val Val             580 585 590 Val Gly Asp Phe Asn Thr Gly Cys Thr Ser His Glu Val Asn Thr Leu         595 600 605 Met His Glu Ala Gly Met Val Leu Ile Gly Asn Ala Gly Ile Asp Cys     610 615 620 Ile Leu Ala Lys Arg Phe Asn Gly Thr Ala Gln Thr Phe Asp Ala Ala 625 630 635 640 Pro Ser Asp His Pro Gly Leu Asp Ala Ser Leu Ser Thr Asn                 645 650 <210> 192 <211> 1965 <212> DNA <213> Microbulbifer degradans <400> 192 gtgcgttttc agtatgcgtt gtgcacagac agtggcggta caattaaaaa taatcaaagt 60 tgggtatcta ttatgttgcg aattcatcta gccatgccaa atatggcctt ttcactatta 120 cagcactatc ttttaaacac attttctggg gtatccatat gggtactttg tggtttattg 180 atgggggcat acgttaatgc cgccacacaa gagatcaacg gcaaccagtt acaagtgaca 240 tgccaaagcg aaaccttttg cgatattcat tatcgtttga ataatggcag agaattaaat 300 atagcgatgg cgtcgttagg taatggtgag tacgcacata cgatttctaa tcttatttct 360 ggcgatgtga ttgtttacta ccttacgtac caacaaaacg gtttggccta tgactctgag 420 cgcctgacgc acatatatgg gggggctagc tccagcgctg gcgcagatcc ctatttgggg 480 tttagagcac ctattccagg gactattctc gctgtgaact acgatactgg aggcgaaggc 540 atcgcttttc atgatgccac aataggaaac tctggtggtc aataccgcga cgaaagtgtg 600 gatatagaga gcgcttcgat tggtggcttt aatgtggggt gggttgcttc cggcgagtgg 660 ctgcaatact ccgtggatgt ggcgagagct ggcagtttcg atattgttgc gcaaatcgcc 720 tctcctaatt ctgggggcag ccttcattat gaaattacag gggcaaccaa tgttcaatct 780 gacgcggtgc aatttgcgaa tacaggtggt tggcaggttt gggcacacaca agcggctgcg 840 aaggtacagc tgaatgcagg tgctcatacc attcggttag tgtttgaatc agcagggttt 900 aatattcatt ctctgctggt aagcgaagcg agctctagtt tcgggaaagt tgaggcagaa 960 agttttgatt ccatgagtgg cgtagtgtcg gaggcgacaa gcgtaggcta ttttgatcgc 1020 ggtgattgga tgaaatacag cgcggtcagc tttggtaatc ttgccaagag cataacgtta 1080 tccgtcgctg gtgaatacaa taatggtata gcagaactta gattagacag tgtcagtggg 1140 ccggtgattg gcacctactc aatggaatcg acaggtggtt gggctagttt taagccaaag 1200 agttttaata tcgttcatac cttgggagtg catgatcttt atattgttgg taaaaatggc 1260 agtggcattt ttaatttaga ctattttcaa ttatctgcag atgtcgtaga agagaccaac 1320 cctgctacag cggtaaaagc gatgtcgctg aatatatatg ggtgggcaac aatgcctcaa 1380 aatgctgata agtatgcagc gcttattcgt tctcggggtg ttgatgttgt gggtattcaa 1440 gagggggtag aagattggct tattgggccg ggttttccca caaattactc caaagcagat 1500 gctcttggtg ctgccttagg agcttgttgg cagcagcgct atcaaatttt tattaatatt 1560 tgtgaaggaa atagcttcgt gtcgaatcgt cgattcgaca tgaccgatgg gcctaatgca 1620 acgcgcacag gcgagtctgc acgaataaac aaaaatgggt tcgaatacgc agtcttaacg 1680 gtgcactggg atcaccaaag tggcgcagct aaagttgcta atgcacatga aacagccgcc 1740 gaggttaatt actacggtgc attgcctacc gttgttgtgg gcgatttcaa cactggctgt 1800 acaagccatg aagtaaatac acttatgcat gaagctggaa tggttttgat tggtaacgcc 1860 ggcattgatt gcattttagc taagcgattc aatggtacgg cacaaacatt tgatgctgca 1920 ccatctgatc accctggctt ggatgcgtca ctcagtacca actag 1965 <210> 193 <211> 550 <212> PRT <213> Microbulbifer degradans <400> 193 Met Lys Ser Cys Cys Ile Lys Phe Phe Thr Thr Leu Cys Thr Ala Val 1 5 10 15 Tyr Val Leu Gly Cys Gly Ala Leu Ala His Ala Gln Thr Gly Pro Ala             20 25 30 Gly Tyr Asn Tyr Ala Ala Ala Glu Asn Glu Thr Val Tyr Leu Asn Gly         35 40 45 Thr Thr Asn Val Ala Tyr Gly Ala Asn Gly Ser Phe Tyr Tyr Ala Tyr     50 55 60 Asn Gln Thr Gly Ser Val Asn Cys Ser Asn Gln Thr Phe Gly Asp Pro 65 70 75 80 Ile Phe Gly Val Arg Lys Ala Cys Tyr Thr Gln Gln Val Ala Ser Asn                 85 90 95 Asn Pro Pro Ser Val Ser Phe Ala Ser Pro Thr Gly Asn Leu Thr Val             100 105 110 Asp Glu Gly Tyr Ala Leu Ser Val Thr Val Asn Ala Ser Asp Ser Asp         115 120 125 Gly Ser Ile Ala Ser Val Glu Leu Phe Ile Asn Asn Gln Leu Val Arg     130 135 140 Gln Glu Leu Tyr Ala Pro Tyr Glu Trp Gly Ala Ala Ala Glu Pro Asp 145 150 155 160 Glu Leu Asn Gly Leu Pro Val Gly Thr His Thr Ile Lys Ala Val Ala                 165 170 175 Thr Asp Asn Asp Gly Asp Thr Lys Gln Ala Ser Phe Lys Leu Thr Val             180 185 190 Arg Gly Ala Ala Val Asp Val Pro Gly Leu Val Gln Ala Glu Asp Tyr         195 200 205 Thr Gly Phe Tyr Asp Thr Thr Asn Gly Asn Thr Gly Gly Ala Tyr Arg     210 215 220 Asn Asp Asn Val Asp Val Glu Thr Thr Ser Asp Ser Asn Gly Gly Tyr 225 230 235 240 Asp Val Gly Trp Phe Ala Ala Asn Glu Trp Leu Glu Tyr Pro Ile Asn                 245 250 255 Val Thr Glu Ala Gly Asn Tyr Val Leu Glu Ala Arg Val Ala Ser Ala             260 265 270 Val Gly Gly Gly Met Phe Thr Ala Glu Ile Asn Gly Asn Asn Ser Ser         275 280 285 Thr Phe Ser Ile Gly Asn Thr Gly Gly Trp Gln Asn Trp Gln Thr Leu     290 295 300 Asn Asn Asn Ile Gly Asn Leu Ser Thr Gly Lys Lys Thr Leu Arg Ile 305 310 315 320 Gln Ala Gln Ser Gly Asn Phe Asn Leu Asn Trp Leu Arg Leu Lys Arg                 325 330 335 Ala Thr Thr Ser Val Cys Thr Leu Asn Thr Pro Ala Glu Asn Ile Pro             340 345 350 Thr Pro Phe Asn Leu Phe Thr Val Ile Asp Thr Asp Leu Asn Arg Tyr         355 360 365 Glu Phe Cys Lys Ala Ser Lys Trp Phe Glu Glu Ser Asn Gly Lys Gln     370 375 380 Val Phe Lys Leu Phe Thr Gly Asp Asn Leu Ala Asp Asn Val Pro Gly 385 390 395 400 Ala Arg Val His Ala Arg Thr Glu Ala Gly Gln Gly Leu Lys Phe Lys                 405 410 415 Ala Gly Ser Thr Trp His Thr Phe Glu Ala Arg Met Lys Pro Ser Lys             420 425 430 Lys Leu Asp Tyr Thr Tyr Thr Ile Ala Gln Leu Phe Ala Gly Cys Cys         435 440 445 Gly Pro Gln Leu Arg Ile Glu Val Lys Ser Asn Gly Arg Ile His Met     450 455 460 Gly Ser Arg Gly Asn Gly Asn Ile Arg Ile Ser Asp Asp Gln Asp Tyr 465 470 475 480 Ala Asn Gly Ser Arg Ser Phe Lys Ile Lys Ile Arg Thr Asn Gly Asp                 485 490 495 Gln Phe Glu Val Tyr Phe Asn Ser Ser Lys Lys Phe Ser Gly Arg Thr             500 505 510 Asp Glu Ala Lys Asn Gly Asn Thr Ser Ala Leu Tyr His Phe Arg Trp         515 520 525 Gly Val Tyr Ser Asn Glu Val Met Ser Glu Asp Leu Ser Asn Thr Val     530 535 540 Thr Glu Ile Ile Arg Asn 545 550 <210> 194 <211> 1653 <212> DNA <213> Microbulbifer degradans <400> 194 atgaaatcat gttgtatcaa gttttttacc acactctgta ctgctgttta tgtactgggt 60 tgtggcgcgt tagcgcatgc gcaaactggc ccggcgggtt ataactacgc tgctgccgaa 120 aacgaaacgg tgtatttaaa cggaaccacc aacgttgcct acggcgcgaa tggctcgttt 180 tattacgctt acaatcaaac cggttcggtt aattgctcta atcaaacctt tggcgaccct 240 atttttgggg tgcgcaaagc gtgttacacc cagcaagtgg cgagcaataa cccgccgtcg 300 gtttcgtttg ctagcccaac gggcaaccta acagtcgacg aaggttatgc tctttctgta 360 accgtgaacg ccagcgatag cgacggcagc attgccagtg tagagctgtt tattaacaac 420 caattagtac gacaagagct ttacgcacct tacgagtggg gcgcagctgc tgagccagac 480 gaattaaatg gcctacccgt tggtacacac acaataaaag cggtagccac cgataacgac 540 ggcgacacca aacaagctag ctttaagcta acggtacgcg gtgcggcggt agatgttcct 600 ggcttggtac aagcggagga ttacacaggc ttttacgaca caaccaatgg caacactggt 660 ggtgcgtatc gcaacgacaa tgtagacgta gaaaccacaa gcgatagtaa cggtggttac 720 gacgtaggtt ggtttgcggc aaacgaatgg ctggaatacc ccattaacgt taccgaagcg 780 ggcaattatg tattagaagc acgcgtcgca tctgctgtag gcggtggtat gtttaccgca 840 gaaataaatg gcaacaacag cagtacattt agcataggca ataccggcgg ctggcaaaat 900 tggcaaaccc tgaataacaa tattggcaat ttaagcaccg gtaaaaaaac gttacgcatt 960 caagcgcaaa gcggaaactt taatttaaac tggctgcgcc tcaagcgtgc cacaacctct 1020 gtgtgcacac ttaatactcc tgccgaaaac attccaacgc catttaattt atttaccgtt 1080 atcgatacag atttaaaccg ctacgaattc tgtaaagcgt ctaaatggtt tgaggaatct 1140 aacggtaaac aagtgtttaa attatttacc ggcgacaacc tagcagataa cgtacctggc 1200 gcccgcgtac atgctcgcac agaagctggc caagggctta agtttaaagc aggctccaca 1260 tggcacacct ttgaagccag aatgaaaccc agcaaaaagt tagactacac ttacaccatt 1320 gctcaattgt ttgccggctg ttgcgggccg cagttgcgca ttgaagtaaa atctaacgga 1380 cgcatccaca tggggtcgcg cggtaacggc aatattcgta ttagtgacga ccaagattac 1440 gccaacggct ctagatcgtt caaaattaaa attcgcacca atggcgatca gttcgaagtg 1500 tattttaaca gcagcaaaaa gttcagcggc cgcacagacg aagccaagaa cggcaatacc 1560 agtgcgcttt accacttccg ctggggtgta tattccaacg aagtaatgag cgaagattta 1620 tctaacactg ttacagaaat tattcgaaat taa 1653 <210> 195 <211> 500 <212> PRT <213> Microbulbifer degradans <400> 195 Met Phe Ala Ser Asn Leu Glu Asp Pro Thr Tyr Tyr Ile Lys Tyr Lys 1 5 10 15 Gly Val Tyr Met Asn Thr Asn Arg Lys Gln Val Asn Gln Asn Leu Ile             20 25 30 Ala Leu Leu Gly Leu Met Leu Leu Met Leu Phe Thr Pro His Gly Tyr         35 40 45 Ala Gln Asp Gln Cys Asn Thr Thr Leu Glu Cys Lys Ile Leu His Gly     50 55 60 Asp Thr Ala Thr Asp Cys Lys Asn Ser Arg Ser Asp Asn Ser Ile Cys 65 70 75 80 Met Cys Gly Ser Thr Glu Cys Ala Val Asp Asn Pro Gln Pro Glu Pro                 85 90 95 Ser Asn Ser Ala Ala Val Pro Gly Leu Ile Gln Ala Glu Asp Phe Thr             100 105 110 Asp Tyr Tyr Asp Val Thr Ala Ala Asn His Gly Gly Ala Tyr Arg Ser         115 120 125 Thr Gly Val Asp Ile Gln Val Thr Thr Asp Thr Asn Gly Gly Tyr Asn     130 135 140 Val Gly Trp Ile Ala Ala Asn Glu Trp Leu Glu Tyr Asn Ile Asn Val 145 150 155 160 Leu Gln Ala Gly Asn Tyr Thr Ala Asn Ile Arg Val Ala Ser Asn Asn                 165 170 175 Gly Val Gly Met Tyr Ser Leu Ala Val Asp Gly Val Thr Val Ser Gly             180 185 190 Thr Asn Thr Val Asn Gly Thr Gly Gly Trp Gln Val Trp Ile Thr Gln         195 200 205 Thr Ala Asn Leu Gly Tyr Leu Thr Gln Gly Glu His Thr Leu Arg Ile     210 215 220 Ala Val Gln Ala Gly Asn Phe Asn Ile Asn Trp Leu Glu Leu Leu Leu 225 230 235 240 Ala Gly Thr Gln Gln Pro Asp Met Leu Gly Val Phe Asn Lys Ser Arg                 245 250 255 Asp Leu Leu Leu Ala Asn Phe Asp Ser Arg Pro Asp Pro Asp Asp Ile             260 265 270 His Ser Val Ala Ala Leu Ala Thr Met Leu Lys Asp Ser Arg Phe Ser         275 280 285 Asn Val Gln Tyr His Ala Val Ser Gly Ala Tyr Gly Ile Gln Gly Gly     290 295 300 Asp Tyr Ile Glu Ala Thr His Leu Phe Asn Leu Ala Phe Gly Ala Gly 305 310 315 320 Asn Trp Ser Asn Ala His Thr Asn Arg Asp Val Ala Leu Thr Thr Val                 325 330 335 Tyr Asn Lys Val Ala Ala Thr Leu Thr Asn Gly Gly Asp Ile Trp Val             340 345 350 Gln Glu Ala Gly Gln Ser Asp Phe Ser Ala Asp Leu Val Arg Arg Ile         355 360 365 Lys Gln Gln Leu Pro Ala Ile Asn Thr Gln Thr Arg Ile His Ile Val     370 375 380 Gln His Ser Asn Trp Asn Gln Asp Lys Thr Thr Pro Ala Asp Leu Thr 385 390 395 400 Tyr Val Lys Asn Gln Thr Asp Tyr Lys Lys Ile Ala Asp Gly Asn Ser                 405 410 415 Thr Gly Asn Gly Thr Pro Gly Phe Asn Ser Ser Ser Ser Ala Asn Trp             420 425 430 Asn Arg Ala Leu Asn His Ala Gln Val Gly Ala Ile Trp Gln Glu Ala         435 440 445 Lys Arg Ile Ala Asp Asn Ala Ile Ala Asn His Gln Gly Trp Gln Asn     450 455 460 Pro Asn Ile Arg Asp Gly Gly Met Asp Phe Ser Asp Ala Val Glu Asp 465 470 475 480 Cys Trp Ile Phe Gly Phe Asn Ser Leu Thr Asn Ile Asn Ser Phe Phe                 485 490 495 Asp Glu Phe Leu             500 <210> 196 <211> 1503 <212> DNA <213> Microbulbifer degradans <400> 196 atgtttgcta gcaacctcga agaccctact tactatataa aatataaagg tgtttacatg 60 aatactaata ggaaacaagt caaccaaaac ctcatcgcgc tattgggctt aatgttgcta 120 atgctattta cacctcatgg ctatgcgcaa gaccaatgca acaccacgct tgagtgcaaa 180 atactccacg gcgatacagc cacggattgt aaaaatagtc gctcagataa cagtatttgc 240 atgtgtggaa gtactgaatg tgcagtagat aatccacaac ccgaacctag caattccgcc 300 gcagtaccgg gcctaataca agcagaagac tttaccgatt actacgatgt aacggcggct 360 aatcacggcg gcgcttaccg ctccacaggc gtagacattc aagttaccac agacaccaat 420 ggcgggtaca acgtaggctg gatagccgct aatgaatggc tagagtacaa cataaacgta 480 ctgcaagctg gtaactacac cgcgaatatt cgtgttgcat ctaataatgg tgttggcatg 540 tatagcttgg cggtagatgg tgtaacggta agtggcacca acaccgttaa tggcacgggt 600 ggctggcaag tttggattac ccaaaccgca aaccttggct acttaaccca aggcgagcat 660 acgttgcgta tagcagtaca ggctggcaat tttaatatta actggttaga gctgctgcta 720 gcgggtactc agcaaccaga tatgcttggt gtatttaata aaagccgcga cctgttgtta 780 gcaaatttcg atagccgccc agacccagac gatattcatt cagtagcggc cctggcaaca 840 atgctaaaag attcccgttt tagcaatgtg caataccacg cagtatcggg tgcttacggt 900 atacaaggtg gtgattacat tgaagctaca catttgttta acttagcctt tggtgccggt 960 aattggtcca acgcgcacac caatagagat gtggcgctta caactgttta caacaaagtt 1020 gccgcaacgt taaccaatgg tggtgatatt tgggttcaag aggccggtca atccgacttt 1080 agcgccgatt tggtacgcag aatcaaacaa cagttacccg ctattaatac gcaaacgcga 1140 attcatatag tgcagcatag taactggaac caagacaaaa ccactccagc cgatttaacc 1200 tatgtaaaaa accaaaccga ctacaaaaaa attgctgatg gcaattccac aggcaacggc 1260 acgcctgggt ttaactcatc cagcagcgcc aactggaacc gcgcactaaa ccacgcgcaa 1320 gtaggcgcaa tttggcaaga agcaaaacgc atagccgaca atgccatcgc caaccaccaa 1380 ggctggcaaa accccaatat tcgagacggc ggtatggact tctcggatgc agtcgaagac 1440 tgctggatat tcggcttcaa ctcactcaca aatataaaca gcttttttga tgagttttta 1500 tag 1503 <210> 197 <211> 473 <212> PRT <213> Microbulbifer degradans <400> 197 Met Gln Leu Ile Gln Leu Leu Lys Thr Met Gly Ile Ser Arg Leu Cys 1 5 10 15 Ile Phe Leu Phe Gly Ala Val Leu Ala Ser Thr Met Leu Ala Gly Cys             20 25 30 Gly Gly Ser Ser Ser Ser Glu Lys Ser Ser Gly Gln Val Val Thr Glu         35 40 45 Pro Glu Ser Glu Pro Glu Ser Glu Pro Glu Ser Glu Pro Glu Ser Glu     50 55 60 Pro Glu Ser Glu Pro Glu Ser Glu Pro Glu Ser Glu Pro Asp Pro Ala 65 70 75 80 Pro Asp Thr Ala Gln Asp Met Arg Ser Glu Lys Arg Gly Leu Ala Tyr                 85 90 95 Gly Tyr His Ser Glu Asn Asp Leu Lys Ala Met Gln Gly Lys Val Lys             100 105 110 Trp Trp Tyr Asn Trp Asp Thr Gln Ala Asp Ala Asn Val Lys Glu Asn         115 120 125 Tyr Ala Ser Tyr Gly Tyr Asp Phe Val Pro Met Ala Trp Asp Glu Asn     130 135 140 Phe Asn Glu Glu Ala Leu Arg Ser Phe Leu Asp Asn His Pro Asp Val 145 150 155 160 Lys Tyr Leu Leu Gly Trp Asn Glu Pro Asn Phe Met Glu Gln Ala Asn                 165 170 175 Leu Thr Pro Ala Glu Ala Ala Ala His Trp Pro Val Leu Glu Ala Ile             180 185 190 Ala Gln Asp Tyr Asn Leu Lys Leu Val Ala Pro Ala Val Asn Tyr Ser         195 200 205 Pro Gly Asn Val Asp Ile Pro Gly Thr Asp Asp Asp Tyr Asp Pro Trp     210 215 220 Leu Tyr Leu Asp Ala Phe Phe Glu Ala Cys Glu Gly Cys Gln Val Asp 225 230 235 240 Tyr Ile Ala Val His Cys Tyr Met Lys Tyr Glu Ser Ala Phe Ser Trp                 245 250 255 Tyr Val Gly Glu Phe Glu Arg Tyr Asn Lys Pro Ile Trp Val Thr Glu             260 265 270 Trp Ala Gly Trp Asp Asp Gly Gly Pro Ala Asn Met Gly Glu Gln Met         275 280 285 Asn Phe Leu Ser Asp Thr Val Arg Trp Met Glu Ser Asn Asp Asn Ile     290 295 300 Tyr Arg Tyr Ser Trp Phe Leu Gly Arg Ser Ser Glu Gly Tyr Asp Gln 305 310 315 320 Phe Pro Tyr Leu Asp Val Leu Leu Ala Asp Gly Glu Leu Thr Pro Leu                 325 330 335 Gly Ser Val Tyr Thr Ser Ile Pro Ser Asn Asp Phe Arg Tyr Lys Ile             340 345 350 Pro Ala Arg Ile Glu Ala Glu Gly Ala His Ser Leu Thr Gly Phe Lys         355 360 365 His Leu Ala Thr Thr Asp Thr Thr Gly Leu Ala Lys Leu Ile Ala Ala     370 375 380 Ser Asn Glu Val Ala Glu Tyr Lys Leu Asn Val Glu Glu Gly Gly Asp 385 390 395 400 Tyr Thr Leu Ala Leu Arg Leu Ala Ser Ser Ala Asn Ser Asp Ile Ala                 405 410 415 Ile Arg Val Asp Gly Leu Leu Val Tyr Thr Phe Glu Asp Ile Asn Thr             420 425 430 Gly Gly Val Glu Ala Trp Met Thr Phe Ser Ser Thr Pro Ile Ser Leu         435 440 445 Thr Ala Gly Asp His Ile Leu Arg Val Glu Ser Lys Ser Ser Arg Phe     450 455 460 Gly Phe Asn Trp Leu Glu Leu Thr Asn 465 470 <210> 198 <211> 1422 <212> DNA <213> Microbulbifer degradans <400> 198 atgcagttaa ttcagctact aaagacgatg ggtattagtc ggctatgtat ttttttattt 60 ggtgccgtac tcgctagcac aatgctagct ggttgcggtg gttcttcaag ttcagaaaag 120 tcctcaggcc aagtggtgac tgagcccgaa tctgaaccag agtcagaacc agagtcagaa 180 ccagagtcgg agccagaatc tgaaccagag tcggagccag aatccgagcc ggacccagcg 240 cccgacacag cgcaagatat gcgcagcgaa aaacgcggct tagcctatgg ctaccacagt 300 gaaaacgacc tcaaagctat gcagggtaaa gtgaagtggt ggtacaactg ggatacccaa 360 gcggacgcga acgtaaaaga gaattacgcc agttatggct acgattttgt tcctatggcg 420 tgggacgaaa actttaacga agaagcgctg cgcagctttt tagataatca ccccgatgtg 480 aaatacctgc tgggctggaa tgaaccaaac ttcatggaac aggccaacct cacaccagcg 540 gaagccgcag cccattggcc cgtgctagag gccatcgcgc aggattacaa cctaaaatta 600 gtcgcccctg cggtgaacta cagccccggt aatgttgata ttccaggtac cgatgatgat 660 tacgaccctt ggctatacct cgatgccttc tttgaagcgt gtgaaggttg ccaagtagat 720 tacattgccg tgcattgcta tatgaaatac gaaagcgctt tcagttggta tgtgggtgaa 780 tttgagcgct acaacaaacc tatttgggta accgagtggg cagggtggga cgatggcggc 840 ccagcgaata tgggtgagca aatgaacttc ttgtccgata ctgtgcgctg gatggagagc 900 aacgataata tatatcgcta ttcttggttt ttggggcgca gtagtgaagg ctacgatcag 960 ttcccatacc tagatgtttt actggccgat ggcgaactaa caccgttggg tagtgtgtat 1020 acctctattc cgtccaacga ttttcgctac aaaatacccg cgcgtattga ggccgaaggc 1080 gcccatagct taacgggctt caaacactta gccacaaccg atactacagg tttagctaag 1140 ttaatagccg cgtctaacga agtagcagag tacaaattaa acgtggaaga ggggggcgat 1200 tacaccttag ctttacgttt ggcttcatct gcaaacagtg atattgccat ccgtgtggat 1260 ggcttattgg tgtacacctt cgaagatatt aataccggcg gtgttgaggc gtggatgacc 1320 tttagctcaa cccctattag tttaaccgcg ggtgatcaca tattacgtgt agagtctaaa 1380 tcgtcgcgtt ttggctttaa ttggttagag cttactaatt ag 1422 <210> 199 <211> 465 <212> PRT <213> Microbulbifer degradans <400> 199 Met Lys Leu Leu Ser Ile Thr His Thr Leu Lys Arg Ala Ile Ala Ser 1 5 10 15 Ala Val Phe Val Ala Ser Ala Ala Thr Ala Ser Ile Ala Asn Ala Val             20 25 30 Thr Val Asp Ile Leu Val Leu Tyr Asp Asn Tyr Ser Ala Asn Tyr Phe         35 40 45 Gly Gly Asp Pro Gln Thr Ala Met Asn Gly Trp Ala Asn Asp Met Asn     50 55 60 Ser Ala Leu Lys Ala Ser Gln Ile Asp Met Lys Phe Arg Ile Val Gly 65 70 75 80 Val Arg His His Glu Glu Asp Gly Ala Gly Met Gly Asp Val Leu Gly                 85 90 95 Asn Leu Arg Val Asp Gly Gly Ala Ile Ala Leu Arg Asp Gln Leu Gly             100 105 110 Ala Asp Met Val Ser Gln Leu His Glu Lys Gly Ala Cys Gly Val Gly         115 120 125 Tyr Val Ala Val Asp Lys Asn Tyr Thr Trp Asn Val Thr His Pro Gly     130 135 140 Cys Gly Pro Met Val Met Leu His Glu Phe Gly His Asn Met Gly Val 145 150 155 160 Thr His Ser Arg Lys Gln Gly Asp Gln Gly Gly Thr Arg Tyr Arg Tyr                 165 170 175 Gly Val Gly Tyr Gly Val Gln Asp Val Phe Val Asp Ile Met Ala Tyr             180 185 190 Glu Gly Val Phe Asn Thr Ser Arg Val Asn Val Phe Ser Asn Pro Asn         195 200 205 Leu Asn Cys Arg Gly Leu Pro Cys Gly Lys Pro Val Gly Asp Ser Glu     210 215 220 Glu Ala His Ala Ser Leu Ala Ile His Asn Val Arg Asn Glu Leu Ala 225 230 235 240 Asn Phe Arg Asn Thr Val Asn Ser Gly Gly Pro Val Arg Leu Phe Glu                 245 250 255 His Cys Tyr Tyr Thr Gly Tyr Thr Val Gly Leu Gly Glu Gly Ser Tyr             260 265 270 Arg Leu Ala Asp Leu Met Asn Arg Gly Leu Val Asn Asp Asp Leu Ser         275 280 285 Ser Leu Gln Val Asp Ala Gly Tyr Arg Val Glu Met Phe Gln His Asp     290 295 300 Asn Phe Thr Gly Asn Val Val Thr Arg Thr Gly Ser Asp Asp Cys Leu 305 310 315 320 Val Asp Glu Gly Met Asn Asp Asp Ile Ser Ser Leu Arg Ile Thr Arg                 325 330 335 Val Ser Gly Gly Phe Ser Gln Thr Ile Gln Ala Glu Asn Phe Phe Ala             340 345 350 Asn Asn Gly Val Gln Leu Glu Asn Thr Thr Asp Ser Gly Gly Gly Gln         355 360 365 Asn Val Gly Trp Ile Asp Ala Asn Asp Trp Met Ala Phe Ser Asn Ile     370 375 380 Thr Ile Pro Thr Thr Gly Asn Tyr Arg Ile Glu Tyr Arg Val Ala Gly 385 390 395 400 Phe Gly Gly Thr Leu Ser Leu Asp Leu Asn Gly Gly Ala Ile Val Leu                 405 410 415 Gly Gln Ile Asn Leu Pro Asn Thr Asn Gly Trp Gln Asn Trp Gln Thr             420 425 430 Ala Ser His Thr Val His Ile Asn Ala Gly Thr Tyr Asn Phe Gly Ile         435 440 445 Phe Ala Asn Ala Pro Gly Trp Asn Ile Asn Trp Phe Arg Ile Val Gln     450 455 460 Leu 465 <210> 200 <211> 1398 <212> DNA <213> Microbulbifer degradans <400> 200 atgaaattac tctcaatcac tcacacacta aagcgggcaa ttgcgagcgc agtttttgtc 60 gcgtccgctg ctaccgcctc tatagccaat gcagtaacgg tagatattct tgtgctttac 120 gataattatt cggcaaatta ttttggcggc gacccgcaga cggccatgaa cggttgggct 180 aacgatatga actctgcgtt aaaagccagc caaattgata tgaagttccg cattgttggt 240 gtgcgtcatc acgaagaaga tggcgcgggc atgggcgacg tacttggtaa tttgcgcgtt 300 gacggtggcg caatagccct gcgcgatcaa cttggtgcag atatggtgtc acaactccac 360 gaaaagggtg cctgtggcgt ggggtatgtt gccgtagata aaaattacac atggaacgtt 420 actcacccag gctgcgggcc aatggtaatg ctgcatgaat ttggccataa catgggggtt 480 actcactcgc gtaaacaggg tgatcaaggt ggtacccgct atcgctatgg cgtaggttat 540 ggtgtgcagg atgttttcgt cgatatcatg gcttacgaag gcgtttttaa caccagccgc 600 gttaatgtat tttctaaccc taacctcaat tgcagaggcc ttccgtgtgg taaacccgtt 660 ggcgattccg aagaagccca cgcttcactc gctattcaca atgtgcgtaa cgaacttgcc 720 aattttcgca ataccgtaaa ttctggtgga ccggttcgat tgtttgaaca ctgttattac 780 acgggttata cagttggctt aggcgaaggt agctatcgct tagccgattt gatgaaccgc 840 ggtttggtaa acgacgattt gtcatcgttg caagttgatg caggctatag agtggaaatg 900 tttcaacacg acaactttac cggcaatgtt gtaacgcgca ccggcagcga cgattgttta 960 gtcgatgaag gcatgaacga cgatataagt tcactgcgta taacacgagt aagtggcggg 1020 tttagtcaaa ctatccaagc agagaatttt ttcgctaaca acggcgtgca actagagaat 1080 acaaccgata gcggtggcgg ccaaaacgta ggttggattg atgctaacga ttggatggct 1140 ttcagcaaca ttaccattcc aaccaccggt aactaccgta tcgaataccg cgttgcaggt 1200 tttggtggca cgctttcgct cgacttaaat ggcggcgcta tagtgctagg ccaaattaat 1260 ttacccaata ccaatggctg gcagaattgg caaaccgcat cgcacactgt acacattaat 1320 gctggcactt ataatttcgg tatttttgct aacgcacccg gctggaatat caattggttc 1380 cgtatagtac agctttaa 1398 <210> 201 <211> 1024 <212> PRT <213> Microbulbifer degradans <400> 201 Met Lys Lys Pro Pro Ser Arg Tyr Ser Thr Leu Ala Ile Thr Leu Cys 1 5 10 15 Met Ser Leu Ser Gln Ala Ala Ile Ala Lys Asp Ile Tyr Val Ala Pro             20 25 30 Thr Gly Asp Asp Ala Gly Ala Gly Ser Phe Ser Ser Pro Tyr Gln Thr         35 40 45 Leu Ala Lys Ala Ala Gln Thr Ala Gln Ala Gly Asp Val Val Tyr Leu     50 55 60 Arg Glu Gly Thr Tyr Gln Glu Thr Leu Arg Pro Ala Asn Ser Gly Thr 65 70 75 80 Ala Ser His Pro Ile Val Phe Gln Ser Tyr Gln Asn Glu Lys Val Ile                 85 90 95 Ile Ser Ala Met Glu Ala Leu Ser Gly Trp Gln Gln Asp Thr Ser Asn             100 105 110 Ile Tyr Lys Thr Thr Val Asn Trp Asp Leu Gly Gln Glu Asn Phe Val         115 120 125 Met His Lys Ser Thr Ala Leu Asp Leu Ala Arg Trp Pro Asn Asn Thr     130 135 140 Asp Ala Asp Pro Phe Thr Leu Asn Ser Lys Arg Asn Thr Gly Gly Ser 145 150 155 160 Gly Pro Glu Val Gly Gln Gly Ala Tyr Ile Glu Tyr Ala Ala Gly Leu                 165 170 175 Pro Asn Ile Asn Trp Thr Gly Gly Thr Val Phe Tyr Tyr Gly Asp Lys             180 185 190 Pro Gly Gly Gly Trp Leu Ala Trp Arg Glu Thr Ile Val Ser His Thr         195 200 205 Gln Thr Arg Ile Asn Ile Asp Leu Ser Lys Lys Asn Pro Ala Trp Val     210 215 220 Arg Thr Ala His Asp Pro Ala Ser Gly Gly Glu Phe Tyr Leu Met Gly 225 230 235 240 Val Lys Gly Ala Leu Asp Tyr Gln Asn Glu Trp Tyr Phe Asp Ser Asn                 245 250 255 Thr Arg Glu Leu Phe Val Gln Leu Pro Asn Gly Ala Arg Pro Gln Asn             260 265 270 Gly Asp Ile Gln Phe Arg Lys Arg Leu Gln Thr Ile Asn Leu Ala Asn         275 280 285 Arg Ser His Ile His Ile Lys Asn Ile Ala Val Phe Gly Gly Ala Ile     290 295 300 Glu Ile Thr Asn Asn Ala Asn Ser Asn Leu Leu Ser Gly Val Ser Ser 305 310 315 320 Phe Tyr Gly Asn Ala Thr Leu Gly Val His Thr Gly Phe Ser Ala Pro                 325 330 335 Ser Tyr Ser Val Lys Ile Gln Gly Ser Asp Asn Arg Ile Glu Asn Ser             340 345 350 Glu Ile Ala Tyr Gly Ser Gly Thr Gly Ile Tyr Asp Ser Gly Thr Arg         355 360 365 Ser Gln Ile Val Asn Asn Tyr Ile His Asp Phe Asn Thr Leu Gly Asp     370 375 380 Tyr Asn Ala Pro Val Asn Ala Arg Gly Gly Ser Asn Thr Leu Val Lys 385 390 395 400 Asn Asn Arg Ile Ser Arg Gly Gly Arg Asp Thr Ile Gln Ala Phe Asn                 405 410 415 Arg Asp Ser Glu Trp Ser Tyr Asn Asp Val Ser His Ser Asn Leu Ile             420 425 430 Ala Asp Asp Cys Gly Leu Phe Tyr Thr Val Gly Gly Pro His Asn Val         435 440 445 Glu Ile His His Asn Trp Phe His Asp Ala Tyr Ser His Gly Asn Lys     450 455 460 Asn Lys Ala Ala Gly Ile Tyr Leu Asp Asn Asp Ala Arg Gly Phe Lys 465 470 475 480 Val His His Asn Val Val Trp Asn Thr Glu Trp Thr Gly Ile Gln Ile                 485 490 495 Asn Trp Asn Gly Thr Asp Ile Asp Val Phe Asn Asn Thr Leu Trp Asn             500 505 510 Asn Ser Ala Ala Met Gly Ala Trp His Lys Ala Gly Thr Ala Phe Ser         515 520 525 Asp Val Arg Val Trp Asn Asn Leu Ser Asn Ser Asn Lys Trp Glu Glu     530 535 540 Gln Ala Asn Lys Gln Asn Asn Leu Thr Gly Thr Gly Asp Pro Phe Val 545 550 555 560 Asn Ser Gln Ala Gly Asp Phe Arg Leu Lys Ala Asn Thr Ala Pro Ile                 565 570 575 Asp Tyr Gly Arg Thr Ile Ala Gly Thr Thr Glu Gly His Ser Gly Ala             580 585 590 Asn Pro Asp Ala Gly Ala Tyr Glu Tyr Gly Ala Thr Ala Trp Lys Ala         595 600 605 Gly Val Thr Trp Asp Ile Thr Lys Gly Ala Ala Asn Arg Cys Tyr Gln     610 615 620 Leu Pro Gly Glu Val Cys Phe Asp Asp Thr Asp Thr Gly Gly Asp Ile 625 630 635 640 Gly Glu Leu Pro Gly Lys Val Glu Ala Glu Asn Tyr Ala His Tyr Tyr                 645 650 655 Asp Thr Thr Pro Gly Asn Ile Gly Gly Ala Tyr Arg Asn Gln Asp Val             660 665 670 Asp Ile Gln Pro Thr Thr Asp Thr Leu Gly Gly Tyr Asn Val Gly Trp         675 680 685 Ile Asn Ala Gly Glu Trp Leu Glu Tyr Asp Ile Asp Val Thr Gln Ala     690 695 700 Gly Arg Tyr Asp Ala Glu Leu Arg Val Ala Ser Lys Leu Gly Ala Gly 705 710 715 720 Gln Val Ala Ile Ala Ile Asp Gly Val Ala Arg Gly Glu Ala Leu Thr                 725 730 735 Ile Gln Ser Thr Gly Asp Trp Gln Asn Trp Ala Thr Leu Thr Thr Gln             740 745 750 Leu Gly Tyr Leu Glu Ala Gly Leu His Thr Leu Arg Val Ser Ala Met         755 760 765 Ser Gly Gly Phe Asn Leu Asn Trp Tyr Asn Phe Thr Lys Gln Val Ser     770 775 780 Phe Gly Glu Thr Ala Val Gly Phe Thr Asn Thr Pro Pro Thr Arg Ile 785 790 795 800 Pro Ala Thr Arg His Gln Ser Phe Ser Val Asp Tyr Val Ala Asn Glu                 805 810 815 Pro Arg Glu Leu Phe Leu Leu Phe Phe Asn Pro Asp Trp Ser Trp Val             820 825 830 Ala Ser Thr Lys Thr Thr Val Val Glu Pro Gly Lys Ala Ser Thr Thr Leu         835 840 845 Gln Leu Asn Leu Pro Phe Val Pro Thr Ala Val Gln Ala Phe Asn Val     850 855 860 Lys Leu Glu Asn Arg Pro Ile Gly Ala Asn Trp Asp Asn Pro Asn Asn 865 870 875 880 Val Glu Ala His Ala Ala Val Thr Thr Gln Ala Ala Pro Leu Gln His                 885 890 895 Asn Gly Gly Phe Glu Asn Gly Gly Thr Ser Gly Trp Asn Gly Tyr Gly             900 905 910 Ser His Ala Leu Ser Thr Glu Ala His Ser Gly Ser Tyr Ala Gly Lys         915 920 925 Val Thr Gly Gly Pro Ser Ala Phe Ser Gln Thr Ile Pro Asn Leu Thr     930 935 940 Pro Asn Thr Thr Tyr Ser Leu Ser Ala Phe Val Lys Ala Arg Ala Gly 945 950 955 960 His Thr Gly Phe Leu Gly Val Lys Glu Tyr Gly Gly Gln Glu Thr Ser                 965 970 975 Leu Ile Val Asn Ser Thr His Tyr Gln Lys Lys Thr Ile Thr Phe Thr             980 985 990 Thr Gly Pro Asn Ala Ser Ser Ala Lys Ile Tyr Leu Tyr Val Arg Asp         995 1000 1005 Asn Asn His Val Ala Phe Ile Asp Glu Leu Val Ile Val Lys Val     1010 1015 1020 Tyr      <210> 202 <211> 3075 <212> DNA <213> Microbulbifer degradans <400> 202 atgaaaaaac cacctagccg ttacagtacg ctggccataa ccctgtgtat gagtttatcg 60 caggccgcta tagcaaagga tatctacgtc gcccctacag gagatgacgc tggagccgga 120 tcgtttagca gcccctatca aacgctggcg aaagcggcac aaaccgcaca agctggtgat 180 gtcgtttacc tgcgcgaagg cacctatcag gaaacgcttc gcccagcgaa ttcaggcaca 240 gccagtcacc ccattgtgtt tcaatcctat cagaatgaaa aagtgatcat cagtgcaatg 300 gaagccttaa gcggctggca gcaagacacc tcaaatattt ataaaaccac ggtgaactgg 360 gatctaggcc aagaaaattt tgtgatgcat aaatctacag cactggattt agcccgctgg 420 ccaaacaata ccgatgcaga ccccttcacg ttaaattcta aaagaaacac agggggaagt 480 ggccccgagg taggccaagg tgcttatata gaatacgcag caggattgcc caatattaat 540 tggactgggg gtaccgtatt ttattatggc gataaacccg gtggcggttg gctagcgtgg 600 cgcgagacga ttgtaagcca cactcaaacc cgtataaata tagacctttc taaaaagaat 660 cccgcgtggg tacgcaccgc acacgacccg gcaagcggcg gagaattcta tttaatgggc 720 gtaaaaggtg ctttagatta tcagaacgaa tggtacttcg attcaaatac gcgagagctt 780 tttgtccaac tgcccaacgg cgcgcgccca caaaatggcg atattcaatt tagaaaacgc 840 ctgcagacca ttaacttggc aaatagaagc cacatacaca ttaaaaacat tgctgttttt 900 ggtggcgcta tagaaataac caataacgct aattctaacc tgctttctgg ggtatccagt 960 ttttatggca atgctacgct cggtgtacac accggatttt cggcgccaag ctacagcgta 1020 aaaattcaag gcagcgataa ccgtatagaa aacagtgaaa ttgcctacgg ctccggtact 1080 gggatatacg acagcggcac ccgcagccaa attgtgaata actacattca cgattttaat 1140 acccttggcg actacaacgc gccggttaac gcgcgcggcg gaagtaatac gttagtaaaa 1200 aataatcgaa tctcccgcgg aggccgcgac accatacaag cttttaatcg agacagcgaa 1260 tggtcgtata acgatgtttc ccacagtaat ttaatagccg atgactgcgg cttgttttac 1320 accgttggcg gaccacacaa tgtggaaatc catcacaact ggtttcacga cgcatattca 1380 cacggcaaca aaaacaaagc cgctggtatt tatttagata acgatgcccg aggttttaaa 1440 gtacaccaca acgtggtgtg gaacaccgag tggacaggca ttcaaataaa ttggaacggc 1500 acggacatag acgtatttaa caacacatta tggaacaaca gtgccgctat gggagcatgg 1560 cacaaagccg ggactgcgtt ttcggatgtt cgagtttgga acaacctctc caacagtaat 1620 aagtgggagg agcaagccaa taagcaaaac aaccttacgg gaacaggcga tccgtttgtg 1680 aacagccaag ccggagattt tagattaaaa gccaatacgg cccctatcga ctatggccgc 1740 actatagcgg gtacaaccga ggggcacagt ggcgccaacc cagatgctgg tgcgtatgaa 1800 tacggcgcaa ccgcttggaa agcaggcgtc acatgggata taaccaaagg cgcagccaac 1860 cgctgctatc aacttcccgg tgaggtttgc tttgatgaca ccgataccgg cggagacatt 1920 ggtgagctac ccggtaaagt agaagccgaa aactacgccc actactacga caccaccccc 1980 ggtaatattg gcggcgccta ccgcaaccaa gatgtagata ttcagcccac caccgataca 2040 ctaggtggct acaacgtagg ctggattaac gctggcgagt ggttagaata cgacatcgat 2100 gtcactcagg cggggcgcta tgatgccgag cttagagtag cctctaaact gggtgcgggc 2160 caagtagcca tagccataga tggggtcgct agaggcgaag cgttaacgat tcagtcgacg 2220 ggcgattggc aaaattgggc aaccctaaca actcaactag ggtatttaga agcagggcta 2280 catacgctgc gggtatccgc tatgagcggt ggcttcaatc taaattggta taacttcact 2340 aaacaagtaa gctttgggga aaccgccgta ggcttcacca acactcctcc tacacgtata 2400 ccagctacaa ggcatcagag cttttctgtg gattatgttg ccaacgagcc gcgagaactg 2460 ttcttattat ttttcaaccc cgactggagt tgggtagcct cgacaaaaac aaccgtagag 2520 cccggtaaag cttctaccac gttgcaatta aaccttccct ttgtgccaac cgctgtgcaa 2580 gcatttaacg ttaaattaga aaatcgcccc ataggcgcca actgggataa cccgaacaat 2640 gtcgaagcgc acgctgccgt taccacgcaa gcagcaccgc tacaacataa cggtggcttc 2700 gaaaacggtg gcacctcggg ttggaatgga tacggtagcc atgcacttag cacagaagcc 2760 cacagcggca gctatgcagg caaagtaacc ggtggcccct ccgcgttttc gcaaactatt 2820 cccaacctta ccccaaacac cacctacagc ctctcggctt ttgttaaagc acgcgctggt 2880 cacacggggt ttttaggcgt taaagaatat ggaggccaag aaacgagcct gatagtaaac 2940 agcacccact atcaaaagaa aaccatcacc ttcaccaccg gccccaacgc cagctcggca 3000 aaaatttatt tatacgttag ggataacaac catgtggcgt ttattgatga gttagtcatt 3060 gtgaaagtgt actag 3075 <210> 203 <211> 912 <212> PRT <213> Microbulbifer degradans <400> 203 Met Lys Lys Ile Leu Phe Leu Ala Leu Gly Leu Leu Ile Ser Gln Ala 1 5 10 15 Ser Phe Ala Gln Gln Thr Ile Ser Ser Leu Ala Glu Phe Ile Ala Leu             20 25 30 Gln Asp Gly Ser Asn Gln Asn Ile Lys Met Ala Pro Gly Thr Tyr His         35 40 45 Ile Ser Ser Ser Ser Lys Ser Leu Phe Pro Gly Gly Asp Trp Arg Ala     50 55 60 Asn Val Glu Gly Asn Trp Pro Gly Leu Phe Lys Phe Ser Gly Asn Asn 65 70 75 80 Asn Thr Phe Asp Leu Thr Gly Val Thr Phe Thr Phe Asp Ser Thr Ile                 85 90 95 Leu Leu Glu Met Pro Asn Leu Val His Ala Asn Leu Met Glu Phe Gly             100 105 110 Gly Ser Gly Asn Val Trp Lys Gly Leu Asn Ile Gln Glu Lys Pro Asn         115 120 125 Ser Lys Gly Glu Tyr Gly Ala Phe Ile His Thr Ser Gly Gly Thr Ile     130 135 140 Ala Val Phe Thr Gly Asp Asn His Lys Val Ser Asp Phe Thr Leu Lys 145 150 155 160 Thr Arg Phe Ser Arg Pro Tyr Gly Leu Gly Ser Leu Tyr Gly Lys Thr                 165 170 175 Gly Asn Ser Ser Ser Thr Leu Pro Gly Val Arg Leu Ser Lys Lys Thr             180 185 190 Ala Met Phe Leu Ile Ser Leu Asp Asp Ser Tyr Phe Glu Asn Val Leu         195 200 205 Ile Asp His Ser Gly Phe Gly His Thr Leu Ala Phe Asn Gly Val Asp     210 215 220 Asn Val Val Phe Asn Thr Val Glu Ile Ila Ala Glu Ser Arg Ser Thr 225 230 235 240 Asp Asp Leu Tyr Ala Asn Gly Ile Gly Gly Thr Asp Arg Asn Gly Val                 245 250 255 Pro Phe Asn Val Leu Phe Asn Gly Asp Glu Leu Ile Gly Thr Asn Phe             260 265 270 Ala Asp Ala Asp Tyr Phe Leu Asn Leu Phe Asp Thr Asp Asn Phe Asn         275 280 285 Gln Cys Gln Asn Met Ser Gly Gly Val Gln Tyr Ser Pro Ile Arg Lys     290 295 300 Gly Tyr Gln Tyr Ser Leu Thr Glu Asp Ser Phe Arg Gly Tyr Tyr Ser 305 310 315 320 Ala Gly Ser Leu Gly Asn Ile Glu Ile Tyr Asn Ala Thr Val Thr Gly                 325 330 335 Ser Arg Ala Gly Val Val Met Glu Tyr Ala Ser Glu Gly Met Ile Val             340 345 350 Asp Gly Met Thr Val Arg Gly Ile Ala Gly His Gly Val Pro Ala Cys         355 360 365 Asp Gly Ala Trp Asn Ser Ala Asn Gly Gly Glu Gly Asp Ala Ser Ala     370 375 380 Tyr Gly Pro Pro Ser Asn Ser Val Leu Lys Arg Ala Lys Ala Asp Ala 385 390 395 400 Ala Tyr Ser Thr Val Leu Glu Ile Pro Asn Phe Val Asp Asn Val Thr                 405 410 415 Ala Asp Ile Glu Val Leu Asp Pro Leu Asn Gly Tyr Asn Arg Pro Ser             420 425 430 Ala Ser Asn Ala Leu Ala Leu Ile Lys Gly Asp Asp His Asp Ile Arg         435 440 445 Leu Trp Lys Arg Asp Asn Gln Ala Leu Thr Arg Asp Leu Val Val Lys     450 455 460 Val Ser Asp Ala Asp Asn Leu Leu Leu Cys Asn Met Thr Lys Gln Gly 465 470 475 480 Val Thr Val Ala Ser Ser Val Thr Asn Ser Thr Ile Tyr Ser Val Gly                 485 490 495 Ser Ile Ser Asn Ser Ser Gly Ser Ser Asn Thr Val Val Lys Leu Ser             500 505 510 Ser Ala Ala Asp Glu Pro Ala Ile Cys Lys Ala Leu Glu Thr Asn Glu         515 520 525 Val Ile Thr Asp Cys Gly Asp Phe Asp Ala Phe Ala Gly Ile Gln Ala     530 535 540 Glu Ala Tyr Cys Asp Met Ala Gly Val Glu Ile Glu Ser Ser Ser Asp 545 550 555 560 Asp Gly Gly Glu Gln Val Gly Tyr Ile Asn Asn Asn Glu Trp Ile Ala                 565 570 575 Phe Asn Asp Val Asp Phe Gly Asn Gly Ala Ser Gly Phe Glu Ala Arg             580 585 590 Val Ser Ser Ala Thr Ser Gly Gly Asn Ile Glu Leu Arg Leu Asp Ser         595 600 605 Gln Thr Gly Ala Leu Ile Gly Thr Cys Ser Val Asn Gly Thr Gly Ser     610 615 620 Trp Thr Thr Tyr Glu Thr Val Ser Cys Asp Ile Gly Gly Val Ser Gly 625 630 635 640 Val Gln Asp Leu Tyr Leu Val Phe Thr Gly Asn Ser Gly Tyr Leu Met                 645 650 655 Asn Val Asn Trp Phe Asn Phe Thr Gln Ala Ala Thr Asn Cys Ser Leu             660 665 670 Pro Trp Ser Asp Ser Asp Phe Ser Val Glu Lys Glu Ile Val Asn Tyr         675 680 685 Ser Ser Gly Ala Ile Asp Ile Ser Cys Ala Ser Asn Val Glu Ile Ser     690 695 700 Met Asn Leu Glu Gly Val Gly Ala Met Glu Asp Ala Asp Tyr Leu Asn 705 710 715 720 Val Tyr Tyr Arg Val Asp Gly Gly Ala Gln Gln Val Ile Ser Glu Asn                 725 730 735 Val Asn Ala Phe Ser Glu Lys Thr Val Ser Val Ser Gly Ile Asn Gly             740 745 750 Ser Ser Leu Glu Ile Ile Ala Asn Val Tyr Thr Ser Tyr Gly Ala Glu         755 760 765 Ile Tyr Thr Ile Ser Asp Met Ser Val Lys Ala Asp Ser Gln Thr Ala     770 775 780 Tyr Ser Leu Glu Val Val Ser Val Leu Ala Ser Ala Asp Asp Gly Asn 785 790 795 800 Val Pro Ala Asn Thr Arg Asp Gly Asp Leu Gly Thr Arg Trp Ser Ala                 805 810 815 Asn Gly Asp Ser Gln Trp Ile Thr Tyr Asp Leu Gly Ser Ser Lys Thr             820 825 830 Val Thr Asp Val Gly Ile Ala Phe Phe Arg Gly Asp Gln Arg Thr Ala         835 840 845 Phe Ile Ser Ile Glu Thr Ser Thr Asn Asn Ser Thr Trp Gln Thr Val     850 855 860 Tyr Ser Asp Glu Gln Ser Ser Ser Thr Thr Glu Ile Gln Asn Phe Asp 865 870 875 880 Val Thr Asp Ser Asn Ala Arg Tyr Val Arg Ile Ile Gly Tyr Gly Asn                 885 890 895 Ser Val Asn Asn Trp Asn Ser Phe Thr Glu Val Glu Ile Gly Arg             900 905 910 <210> 204 <211> 2739 <212> DNA <213> Microbulbifer degradans <400> 204 atgaaaaaaa tcctgttttt ggccttaggc cttttaataa gtcaggcttc gtttgcccag 60 cagacgattt caagccttgc cgagtttatt gctcttcaag atggcagcaa tcaaaacata 120 aaaatggccc ccggcaccta tcatatatca tcatcgtcta aatcactgtt ccccggtggg 180 gattggcggg cgaacgtcga aggtaattgg ccgggcctgt ttaagtttag tggcaacaat 240 aatacgtttg acctgaccgg cgttaccttc accttcgact ccacgatctt gttagaaatg 300 cctaatcttg ttcacgcaaa cctcatggag tttgggggtt ccggtaacgt ttggaagggg 360 ctgaatattc aggagaagcc caacagtaag ggcgagtacg gcgcttttat tcatacctct 420 ggcggcacca tcgctgtatt cactggggat aatcacaaag tttctgattt tactttaaag 480 acacgttttt ctcggcccta tggtttaggt tctctctatg gaaaaacggg taactcgtct 540 agcacgctgc ccggcgttcg cctgtccaag aaaacggcca tgtttcttat tagtttagat 600 gatagttact ttgaaaatgt tctgattgat cacagtggct ttggtcatac gcttgccttt 660 aacggtgtcg ataacgtggt attcaatacc gttgaaatca tcgcagaatc tcgctctacc 720 gatgatttgt atgctaacgg cattggcgga acagaccgca atggtgtgcc ttttaacgtc 780 ctctttaatg gcgatgaact cataggaacg aattttgccg atgccgatta ctttcttaac 840 ctgtttgata cggataactt caatcagtgt caaaacatga gcggtggcgt gcaatattcg 900 cccatccgaa aaggttacca atatagcctg actgaagatt cgtttcgcgg gtactacagt 960 gctggttccc ttggcaatat cgagatttat aacgccaccg ttacgggttc aagagcgggc 1020 gtcgtcatgg aatatgcgag cgagggtatg attgtagacg gtatgacggt tagaggtatt 1080 gccgggcatg gcgtgccagc gtgcgacggc gcgtggaatt cagctaacgg tggtgaaggc 1140 gatgcttccg cctatggtcc tccatcaaac agtgtactta agcgtgcgaa agccgatgcc 1200 gcctattcca ctgttttaga aatccccaat tttgtcgata acgtgacggc cgatattgaa 1260 gtgttggacc cgttaaacgg ttacaaccgt ccttcggcat caaacgcttt ggcgcttatt 1320 aaaggtgatg atcacgatat tcgactttgg aaacgtgata accaagcctt aacccgtgac 1380 ttggtggtta aggtgagtga tgcggataat ctattgctct gtaacatgac aaaacaaggc 1440 gtgactgtcg ccagcagcgt gaccaactcc accatttatt ctgttggtag catcagtaat 1500 tcaagtggca gttctaacac ggtggtaaaa ctgtcaagcg cagctgacga gccagcgata 1560 tgtaaagctc ttgagactaa cgaagttata acggattgcg gtgattttga tgcattcgcc 1620 ggtatccaag ccgaagccta ctgcgatatg gctggcgttg aaattgaatc ctccagcgat 1680 gacggtggcg aacaggttgg ttacattaac aacaacgaat ggattgcatt caacgatgtt 1740 gattttggca atggcgcaag cggttttgaa gcgcgcgtta gtagtgctac ctccggtggc 1800 aatattgagc ttcgtttaga cagccaaaca ggcgcgttaa ttggcacgtg ctctgttaat 1860 ggaacgggca gttggacgac ctatgaaacg gtttcttgcg atatcggtgg cgtcagcggc 1920 gtgcaggatt tgtacctcgt gtttaccgga aatagtggct atttaatgaa tgttaattgg 1980 tttaacttca cgcaagctgc aacgaattgt tctctgcctt ggagtgacag tgacttcagc 2040 gttgaaaaag aaatagttaa ctacagttcc ggtgctattg atatttcatg tgcttccaac 2100 gttgaaattt ccatgaatct agaaggtgta ggcgccatgg aagatgccga ttacttgaac 2160 gtttattacc gcgttgatgg cggtgcccaa caagtgatat ctgaaaatgt gaatgcgttt 2220 tctgaaaaaa ccgtatcggt atcaggtatc aatggcagta gcttagaaat tatcgccaat 2280 gtttatacca gctatggcgc agagatttat acgatttctg atatgagtgt taaagctgat 2340 tcgcaaacag cttattctct agaggttgtt tctgtcttag ccagtgcgga cgatggcaat 2400 gtgcctgcca atacccgtga tggcgacctg ggtacacgct ggtcggctaa tggtgattcg 2460 cagtggatta cctatgatct tggcagcagt aaaactgtga ccgatgtggg catcgccttt 2520 tttagaggcg accaacgcac ggcatttatc agcatcgaaa cctcaaccaa taatagtacc 2580 tggcaaaccg tttattctga tgagcagtca agcagcacaa cagagattca aaattttgat 2640 gtgaccgata gcaatgctcg ctatgtaaga attattggct acggaaatag cgtaaataac 2700 tggaacagtt ttacggaggt cgagattatt ggtcgttaa 2739 <210> 205 <211> 541 <212> PRT <213> Microbulbifer degradans <400> 205 Leu Ala Leu Ser Ala Ser Leu Thr Gln Ala Ala Thr Ile Ser Asn Ser 1 5 10 15 Gly Phe Glu Ser Gly Phe Asp Gly Trp Thr Asp Thr Asp Pro Ser Ala             20 25 30 Leu Ser Ser Asp Ala Asn Asn Gly Ser Arg Ser Ala Lys Ile Thr Gly         35 40 45 Ser Ala Gly Arg Val Asp Gln Asp Val Ala Val Thr Pro Asn Thr Asn     50 55 60 Tyr Gln Leu Thr Ala Tyr Val Leu Gly Ser Gly Arg Val Gly Val Asn 65 70 75 80 Thr Gly Thr Ala Val Tyr Asp Glu Ala Val Asn Thr Ser Ser Trp Ser                 85 90 95 Lys Val Thr Val Asn Phe Asn Ser Gly Ser Ala Asn Ser Val Glu Val             100 105 110 Phe Gly Lys Tyr Asn Ser Gly Thr Gly Arg Phe Asp Asp Phe Ser Leu         115 120 125 Val Glu Thr Gly Thr Pro Thr Pro Thr Pro Thr Pro Thr Pro Thr Pro     130 135 140 Thr Pro Thr Pro Ala Gly Cys Asn Ser Leu Asn Thr Ile Asp Ile Ser 145 150 155 160 Ser Ala Thr Asp Asp Gly Ser His Asp Gly His Gly Pro His Leu Ala                 165 170 175 Val Asp Gly Asp Leu Ser Ala Asp Ser Arg Trp Ser Ser Lys Gly Asp             180 185 190 Gly Lys Ala Ile Thr Leu Asp Leu Gly Ala Glu Ala Thr Val Arg Gln         195 200 205 Leu Lys Thr Ala Trp Tyr Lys Gly Asp Ser Arg Thr Ala Tyr Phe Asp     210 215 220 Val Glu Thr Ser Thr Asp Lys Ser Asn Trp Ser Thr Ala Leu Ser Asn 225 230 235 240 Val Gln Ser Gln Gly Ser Thr Gly Leu Lys Ser Asn Ser Ile Asp Asp                 245 250 255 Val Thr Ala Arg Tyr Val Arg Ile Val Gly His Gly Asn Ser Ser Asn             260 265 270 Thr Trp Asn Ser Leu Ile Glu Ala Gln Val Leu Gly Cys Ala Gly Thr         275 280 285 Val Thr Pro Thr Pro Thr Pro Thr Pro Thr Pro Thr Pro Thr Pro Thr     290 295 300 Pro Thr Pro Thr Pro Thr Pro Ser Gly Ser Lys Ile Pro Glu Ser Ile 305 310 315 320 Thr Asn Ser Asp Val Trp Asp Leu Glu Gly Glu Asn Pro His Pro Leu                 325 330 335 Val Asp Pro Tyr Thr Leu Glu Phe Val Pro Leu Glu Ala Arg Val Thr             340 345 350 Thr Pro Asn Gly Asn Gly Trp Arg His Glu Tyr Lys Ile Ala Ser Ser         355 360 365 Glu Arg Thr Ala Met Thr Ala Thr Tyr Glu Asp Phe Ser Ala Thr Ile     370 375 380 Lys Val Asp Leu Ser Thr Gly Gly Lys Thr Ile Val Ala Gln His His 385 390 395 400 Ala Gly Asp Thr Gly Thr Ile Met Lys Leu Tyr Val Ser Asp Thr Ser                 405 410 415 Glu Ser Gly Phe Phe Asp Ser Val Ala Ala Asn Gly Ile Phe Asp Val             420 425 430 Tyr Val Arg Ile Arg Asn Thr Ser Gly Val Glu Glu Lys Lys Pro Leu         435 440 445 Gly Thr Ile Arg Ser Gly Asp Ser Phe Ser Phe His Val Leu Asn Asn     450 455 460 Tyr Gly Val Val Lys Val Ser Ala Phe Gly Lys Asn Leu Glu Thr Glu 465 470 475 480 Val Glu Asp Asp Ser Ala Ser Tyr Leu Lys Phe Gly Asn Tyr Leu Gln                 485 490 495 Ser Gln Tyr Pro Gln Gly Ser Lys Asp Cys Gly Ser His Gly Asp Ser             500 505 510 Asp Ser Phe Arg Ala Cys Tyr Glu Asp Ile Gly Ile Thr Glu Ala Lys         515 520 525 Ile Thr Met Thr Asn Val Ser Tyr Thr Arg Ile Thr Lys     530 535 540 <206> 206 <211> 1626 <212> DNA <213> Microbulbifer degradans <400> 206 ctcgcgctta gtgcatcttt aacacaggct gcgaccattt ctaactcagg cttcgaaagt 60 ggctttgacg gttggacaga cactgaccct tcggcacttt ctagcgatgc aaataacggc 120 agccgatctg ccaaaattac tggctccgcc ggccgtgtag atcaagacgt tgcggtaacc 180 ccaaacacca actaccagtt aactgcatat gtacttggca gtggacgcgt gggtgttaac 240 accggcactg cagtttatga cgaagccgta aacacaagca gctggagcaa agtaactgtt 300 aactttaact ctggctctgc aaactctgta gaagtgtttg gtaagtacaa tagtggtact 360 ggccgctttg acgatttcag cttagttgag acgggaacac cgactcctac gccaacacct 420 actcctacgc ccacgcctac accagcgggc tgtaacagct taaataccat cgacattagc 480 tctgctaccg atgatggctc tcacgatggt cacggcccac acctagctgt ggacggcgat 540 ttatcagctg attcacgctg gtcttctaaa ggcgacggca aagcgattac tttagactta 600 ggcgccgaag ccacagttcg tcaactaaaa acggcttggt acaaaggtga ctcacgtacc 660 gcttacttcg atgtagaaac gtctaccgat aagagcaact ggtctaccgc tttaagcaac 720 gtgcagtcac aaggtagcac tggccttaaa tcaaacagca ttgacgacgt aacagcgcgt 780 tacgtacgta ttgttggtca cggtaactcg tcgaatactt ggaacagctt gattgaagca 840 caagtattgg gttgtgctgg cactgtaacg ccaacaccta ctcctacgcc tacaccaact 900 ccgaccccaa cgcctactcc tacgccaact ccatctggct caaaaattcc tgaaagcatt 960 acaaacagcg atgtttggga tttggaaggc gagaacccac acccattggt agacccttac 1020 acgttggaat tcgtacctct tgaagcacgc gtaacgactc caaacggtaa cggctggcgc 1080 cacgagtaca aaatcgcttc tagcgagcgt actgcgatga ctgctaccta cgaagatttc 1140 tctgcaacta ttaaagtaga cctatctact ggcggtaaga caattgttgc acagcatcac 1200 gcaggcgaca ctggcaccat catgaaacta tacgtttccg acacaagcga atctggcttt 1260 ttcgatagcg tagcggcaaa cggcattttc gacgtgtatg ttcgtattcg taacaccagc 1320 ggtgttgaag agaagaaacc attgggcaca atccgctctg gcgactcttt cagcttccac 1380 gtacttaaca actacggcgt tgtaaaagta tctgcctttg gtaaaaacct agagacagaa 1440 gtagaagacg attctgcatc ttacttgaag tttggtaact acctacaatc gcaataccca 1500 caaggtagca aagattgtgg ttcacatggc gactccgatt cgttccgtgc ctgctacgaa 1560 gatataggca ttaccgaagc gaaaatcacc atgactaacg tttcttacac gcgtatcact 1620 aagtaa 1626 <210> 207 <211> 1033 <212> PRT <213> Microbulbifer degradans <400> 207 Met Gly Glu Ala Pro Val Lys Lys Gln Ala Tyr Thr Thr Thr Pro Val   1 5 10 15 Lys Pro Asn Ala Leu Gly Leu Ala Ile Arg Thr Leu Ala Leu Gly Gly              20 25 30 Leu Ala Ala Gly Leu Val Asn Val Ala His Ala Asp Leu Leu Ser Val          35 40 45 Asn Lys Thr Ala Thr Ala Ser Ser Glu Met Gln Ala Ala Ala Tyr Ala      50 55 60 Phe Asp Asn Asn Gln Asn Thr Arg Trp Glu Ser Ala His Ala Val Asp  65 70 75 80 Pro Thr Ser Ile Ser Val Asp Leu Gly Glu Thr Tyr Asp Leu Asp Ser                  85 90 95 Ile Val Val His Trp Glu Ala Ala Asn Ala Ala Ser Tyr Thr Ile Glu             100 105 110 Gly Ser Asp Asn Gly Val Asn Trp Thr Gln Ile Gly Thr Tyr Thr Gly         115 120 125 Gly Thr Phe Gly Asn Arg Thr Asp Thr Val Asn Val Asp Gly Asn Tyr     130 135 140 Arg His Val Arg Leu Asn Gly Thr Gln Arg Ser Asp Gly Asn Ala Trp 145 150 155 160 Gly Tyr Ser Ile Trp Glu Leu Glu Val His Gly Thr Glu Val Thr Glu                 165 170 175 Pro Pro Val Val Glu Pro Pro Thr Glu Pro Gly Glu Asn Leu Ala Ile             180 185 190 Tyr Gly Thr Ala Thr Ala Ser Ser Gly Asn Ala Asp Val Ala Ile Asp         195 200 205 Asn Asn Ala Gly Thr Arg Trp Glu Ser Asp His Gly Ile Asp Pro Ser     210 215 220 Ser Phe Thr Leu Asp Leu Gly Ala Thr Tyr Ser Leu Asn Gln Val Val 225 230 235 240 Ile Asp Trp Glu Ala Ala Asn Ala Lys Val Tyr Ala Ile Gln Gly Ser                 245 250 255 Asn Asp Gly Thr Asn Phe Thr Thr Leu Ala Asn Tyr Ser Gly Gly Glu             260 265 270 Phe Gly Thr Arg Thr Asp Thr Leu Asn Ile Ala Gly Asp Tyr Arg Tyr         275 280 285 Val Arg Leu Leu Gly Thr Glu Arg Ser Asp Gly Asn Ala Trp Gly Tyr     290 295 300 Ser Ile Trp Glu Phe Lys Val Tyr Gly Gly Gly Lys Thr Glu Pro Pro 305 310 315 320 Val Thr Glu Pro Pro Val Thr Glu Pro Pro Val Val Glu Pro Pro Ile                 325 330 335 Phe Thr Asp Leu Asn Tyr Gln Pro Leu Phe Asn Asn Thr His Ser Pro             340 345 350 Asp Thr Ala Gln Glu Trp Tyr Thr Lys Pro Asp Gly Thr Val Val Thr         355 360 365 Ile Ala Ser Gly Arg Ala Arg Ser Arg His Glu Ser Glu Asp Ile Phe     370 375 380 Tyr Thr Phe Pro Thr His Tyr Phe Glu His Arg Thr Phe Glu Ile Glu 385 390 395 400 Ile His Asp His Thr Pro Lys Gly Gln Asn Leu Val Glu Val Phe Tyr                 405 410 415 His Pro Glu Tyr Ala Asn Tyr Val Pro Pro Gly Cys Arg Ser Ser Tyr             420 425 430 Ser Asn Val Trp Arg Ala Asp Phe Asn Asn Asn Ala Gly Met Asp Glu         435 440 445 Lys Leu Gln Thr Ala Thr Pro Asp Gly Lys Gly Glu Arg Trp Val Cys     450 455 460 Arg Ile Gln Arg Asp Ala His Asn Gly Asp Asp Gly Ile Leu Asp Val 465 470 475 480 Gly Ser Trp Met Glu Phe Glu Leu Gln Gln Phe Leu Gly Leu Tyr Glu                 485 490 495 Gly Asp Pro Asn Val Arg Gly Gln Ala Val Tyr Tyr Thr Asp Thr Tyr             500 505 510 Arg Phe Lys Leu Gly Gln Pro Gly Ile Tyr Ile Val Gly Asp Glu Ala         515 520 525 Met Glu Glu Lys Ile Arg Ala Gly Gly Arg Ala Thr Ala Pro Tyr Val     530 535 540 Lys Gly Gly Asp Ser Val Pro Val Asn Glu Val Ile Ser Val Asn Gly 545 550 555 560 Asp Asn Thr Leu Thr Tyr Lys Val Met Ala Asn Gly Lys Trp Thr Gln                 565 570 575 Lys Asp Asn Pro Asn Gly Thr Val Val Thr Phe Pro Ile Arg Asp Gly             580 585 590 Ile Glu Val Tyr Asp Asn Tyr Val Val Ala Ser Gly Val Ala Asp Trp         595 600 605 Thr Thr Tyr Phe Arg Glu Ala Leu Asn Ile Gln Trp Asp Thr His Asn     610 615 620 Ala Phe Met Gln Gly Arg Arg Val Phe His Thr Arg Met Asp Thr Gly 625 630 635 640 Val His Glu Glu Val Gly Asn Pro Asp Phe Pro Glu Leu Ala Asn Ile                 645 650 655 Ala Asp Gly Leu Met Val Lys Asn Ser Cys Leu Gly Cys His Val Asn             660 665 670 Asn Gly Arg Gly Ile Ala Pro Gln Asn Gly Ala Leu Leu Asp Thr Leu         675 680 685 Val Val Lys Val Gly Ser Gly Ala Phe Asp Asn Leu Gly Gln Pro Gln     690 695 700 Pro His Ser Tyr Tyr Gly Gly Val Leu Gln Asn Leu Ser Leu Asp Ala 705 710 715 720 Ala Val Pro Ala Glu Gly Ser Val Arg Val Thr Tyr Thr Ala Gln Asn                 725 730 735 Gly Thr Phe Asn Asp Gly Thr Gly Tyr Ser Leu Gln Val Pro Thr Tyr             740 745 750 Ser Leu Glu Met Asn Asp Thr Asn Gly Gly Ala Ile Gln His Ile Ser         755 760 765 Pro Arg Met Pro Gln Asn Ile Thr Gly Leu Gly Leu Leu Glu Ala Leu     770 775 780 Pro Glu Asn Glu Ile Leu Ala Trp His Asp Pro Asp Asp Ser Asn Gly 785 790 795 800 Asp Gly Ile Ser Gly Arg Ala Asn Val Val Thr Ser Pro Glu Thr Gly                 805 810 815 Gln Gln Phe Ile Gly Arg Phe Gly Trp Lys Ala Ser Ser Ala Ser Leu             820 825 830 Arg Asp Phe Ala Ala Thr Ala Leu Ser Gly Asp Met Gly Val Asn Thr         835 840 845 Ser Val Leu Pro Asn Ala Asp Cys Gly Ala Gln Gln Thr Ala Cys Ile     850 855 860 Gln Asn Ser Gly Gln Gly Val Glu Leu Ser Asp Leu Arg Leu Asn Glu 865 870 875 880 Leu Val Val Tyr Leu Gln Ala Leu Gly Ala Pro Ser Arg Arg Pro Glu                 885 890 895 Thr Val Asp Gln Pro Met Val Val Ala Gly Glu Gln Arg Phe Thr Asp             900 905 910 Ile Gly Cys Ala Ser Cys His Arg Pro Glu Met Asn Thr Gly His Lys         915 920 925 His Asp Leu Ala Glu Leu Arg Gly Asn Val Ile Arg Pro Tyr Thr Asp     930 935 940 Met Leu Leu His Asp Met Gly Pro Gly Leu Ala Asp Ser Leu Thr Gln 945 950 955 960 Ala Pro Glu Leu Asn Arg Glu Trp Arg Thr Ala Pro Leu Trp Gly Leu                 965 970 975 Gly Met Asn Leu Ala Val Asn Gly His Asp Asn Leu Leu His Asp Gly             980 985 990 Arg Ala Arg Ser Ile Glu Glu Ala Ile Leu Trp His Gly Gly Glu Ala         995 1000 1005 Gln Ala Ser Asn Asn Ala Tyr Lys Ala Leu Ser Ala Gln Gln Arg Ala    1010 1015 1020 Glu Leu Ile Ala Phe Leu Arg Ser Leu 1025 1030 <210> 208 <211> 3102 <212> DNA <213> Microbulbifer degradans <400> 208 gtgggagaag cacctgtgaa aaaacaagct tatacaacta caccagttaa accaaatgcg 60 ttaggtttag ccattcgcac actggccctt ggtggcctag ctgcgggact tgtaaacgta 120 gcgcatgcag atttattgtc ggtaaataaa actgctacgg ccagcagtga aatgcaagct 180 gcagcttacg cattcgataa caatcaaaat acgcgctggg aaagtgcgca cgcggttgat 240 ccaaccagca taagtgtaga cttgggcgaa acctacgatt tagatagcat tgtcgttcac 300 tgggaagctg ccaacgctgc aagctacacc atagagggct ccgataacgg tgttaactgg 360 acgcaaatag gcacctatac cggtggtacc tttggcaata gaaccgatac cgtaaacgta 420 gatggtaatt accgtcacgt gcgattaaat ggtactcagc gcagcgatgg caatgcatgg 480 ggttactcca tatgggagct ggaagttcac gggacagaag taaccgaacc acctgtagtg 540 gaacctccta cagagcctgg cgagaatttg gcaatatatg gcacggcaac cgccagctct 600 ggtaacgccg atgtagctat tgataataac gctggtacgc gctgggagag tgaccacggc 660 atcgaccctt ctagttttac tttagaccta ggcgcaacct acagcttgaa ccaagtggtt 720 atagattggg aggccgccaa cgccaaggtg tatgctatcc aaggctctaa cgatggcact 780 aactttacta cccttgctaa ttacagcggc ggtgaattcg gcacgcgtac cgatacatta 840 aatattgctg gtgattatcg ctatgtacgc ctgttgggta ctgagcgcag cgatggcaac 900 gcatggggct attctatttg ggaatttaaa gtatatggcg gtggaaaaac tgagccaccg 960 gttaccgagc cacctgtgac agaaccgcca gtggtagagc cccccatttt taccgaccta 1020 aattatcagc cactgtttaa caacacacac agcccagata ctgcgcaaga gtggtacacc 1080 aagcccgacg gtacagtggt aacaattgca agtggccgcg cgcgttcacg tcacgaatcg 1140 gaagatattt tttacacttt cccaacgcat tattttgagc accgtacatt tgaaatagaa 1200 attcacgacc atacccctaa gggccaaaat ttggtggagg ttttctacca cccagaatac 1260 gccaactatg tgccgccggg ttgtcgctct tcttacagca atgtttggcg tgcagacttt 1320 aacaacaacg ccggcatgga tgaaaaactg caaaccgcca ccccagacgg taaaggtgag 1380 cgttgggtat gccgcataca gcgcgacgcg cacaatggcg acgacggcat tttagatgtg 1440 ggttcatgga tggagtttga attacaacaa ttcttaggct tatatgaagg cgaccctaat 1500 gtgcgtggcc aggccgttta ctacaccgat acataccgtt ttaaattagg ccagcccggt 1560 atttatattg tgggcgatga agccatggag gaaaaaattc gtgcgggtgg ccgcgctaca 1620 gcgccttatg taaaaggcgg cgactcggta cctgttaacg aagttatttc ggttaatggc 1680 gacaacacac ttacctacaa agtaatggca aatggtaagt ggacgcaaaa agataacccc 1740 aacggcactg tagttacttt ccctattcgc gatggcattg aggtatacga taactacgta 1800 gtggcgagtg gcgtggccga ttggaccacc tatttccgcg aagcgttaaa tattcagtgg 1860 gatacgcaca acgcatttat gcaggggcgg cgcgtgtttc atacgcgtat ggataccggc 1920 gtacacgaag aagtgggtaa cccagacttt cccgaattgg caaatattgc cgatggttta 1980 atggtgaaaa attcgtgctt gggttgtcat gtaaacaacg gccgcggtat tgctccgcaa 2040 aacggcgcgt tattagatac gctagtcgtt aaagtaggct cgggtgcgtt cgataacctt 2100 ggccagccac aaccacacag ttattacggt ggtgtactgc aaaacctatc actagatgct 2160 gctgtgcctg cagagggttc ggttcgtgta acttacaccg cgcaaaacgg aacctttaac 2220 gacggcacag gctacagctt gcaagtgcct acctattcgt tagaaatgaa cgataccaac 2280 ggcggggcta ttcagcatat ttcaccgcgc atgccacaaa acataactgg tttaggctta 2340 ttagaagcct tacccgaaaa tgaaattttg gcatggcacg acccagatga tagcaatggc 2400 gatggtattt ctggccgcgc taatgttgtt acctcaccag aaacggggca acagtttata 2460 ggtcgctttg gctggaaagc gtcatcagca agtttgcgtg attttgctgc aacggcttta 2520 agtggcgata tgggtgtaaa tacctctgtg ttacccaatg cagattgtgg cgcacaacaa 2580 accgcctgta ttcaaaatag cggtcagggc gtagagctaa gcgacctgcg tttgaatgaa 2640 ctggtggttt acttgcaagc gctgggcgcg ccttcgcgca ggccagaaac tgtagaccag 2700 ccaatggtag tggccggtga gcagcgtttt accgatatag gctgtgcctc gtgtcaccga 2760 ccagaaatga acaccggtca taagcacgat ttagctgagt tgcgtggcaa cgttattcgc 2820 ccctataccg atatgctgtt acacgatatg ggcccaggtt tggcagatag cttaactcaa 2880 gcgccagagc taaatcgcga atggcgtaca gcgccgctgt ggggcttggg catgaacttg 2940 gctgtaaacg gtcacgacaa cttgctccac gatggtcgcg cacgcagtat tgaagaagcc 3000 attttatggc acggtgggga agcgcaagcc agtaataatg cctataaagc actaagtgcg 3060 cagcagcgag cagagttaat agcgttttta cgctcactct aa 3102 <210> 209 <211> 557 <212> PRT <213> Microbulbifer degradans <400> 209 Met Leu His Ile Ser Val Gln Pro Asn Leu Thr Arg Ser Ile Ser Gly 1 5 10 15 Phe Leu Leu Gly Leu Ala Gly Val Val Ser Gly Ser Ala Tyr Ala Gln             20 25 30 Trp Asn Pro Ala Pro Asn Trp Glu Asp Ser Tyr Ser Val Asn Gly Val         35 40 45 Cys Tyr Cys Asn Ser Ser Asn Tyr Asp His Gly Leu Ser Ala Lys Thr     50 55 60 Ala Pro Thr Pro Ile Gly Glu Leu Asn Val Val Asp Ile Cys Thr Asp 65 70 75 80 Ile Lys Ala Val Leu Gly Glu Gly Ala Thr Asn Gly Arg Ile Pro Phe                 85 90 95 Asn Asp Ile Gln Cys Gly Asn Gly Pro Ala Asn Asp Ala Ala Asp Glu             100 105 110 Ala Gly Cys Pro Gly Arg Val Asp Ile Gly Ser Ala Gly Cys Asp Val         115 120 125 Ile Gly Pro Lys Trp Asp Leu Val Ser Val Tyr Gly Pro Trp Pro Asp     130 135 140 Gly Gly Leu Asn Arg Asp Ala Trp Glu Val Ser Ala Ser Asn Gly Ser 145 150 155 160 Gly Ser Ala Gln Leu Ala Leu Asp Gly Leu Ala Ser Thr Arg Trp Ala                 165 170 175 Thr Gly Val Phe Gln Ser Pro Gly Gln Tyr Phe Asp Ile Asp Phe Gln             180 185 190 Asp Ala Leu Thr Phe Asp Ser Ile Val Leu Ala Thr Thr Glu Asn Pro         195 200 205 Glu Asp Tyr Pro Arg Ala Tyr Glu Val Tyr Ile Ser Ser Asn Gly Ser     210 215 220 Asp Trp Gly Val Pro Val Val Thr Gly Ala Gly Asn Gly Ser Thr Thr 225 230 235 240 Thr Ile Glu Leu Asp Thr Val Thr Thr Arg Tyr Leu Arg Ile Leu Gln                 245 250 255 Thr Gly Ser Ser Ser Asn Arg Trp Trp Ser Ile His Glu Phe Asn Ile             260 265 270 Phe Asn Ser Gly Pro Ile Pro Pro Glu Pro Glu Tyr Pro Ala Leu Asp         275 280 285 Arg Ser Asp Trp Thr Val Ser Ala Ser Val Asn Gly Ala Glu Ala Glu     290 295 300 Phe Ala Ile Asp Ser Ser Pro Asn Thr Arg Trp Asp Thr Ala Gln Ser 305 310 315 320 Gln Arg Ala Gly Gln Ser Phe Glu Val Asp Leu Gly Glu Leu Asn Thr                 325 330 335 Leu Ala Ala Ile Glu Leu Asp Ser Ala Gly Ser Ala Asn Asp Tyr Pro             340 345 350 Arg Gly Tyr Ala Val Tyr Val Ser Asn Asp Gly Ser Asn Trp Gly Ser         355 360 365 Ala Ile Ala Ser Gly Ala Ala Val Ser Ala Ser Thr Thr Ile Glu Phe     370 375 380 Thr Pro Val Ser Ala Arg Phe Val Lys Ile Glu Gln Thr Gly Gly Asp 385 390 395 400 Gly His Tyr Trp Trp Ser Ile His Asn Leu Asn Ile Phe Gly Glu Pro                 405 410 415 Ser Asp Asn Pro Gln Pro Thr Ile Glu Leu Leu Asp Ser Thr Pro Trp             420 425 430 Ser Leu Ala Ala Asn Arg Arg Asn Ser Ala Ala Gly Asn Ala Ile Asp         435 440 445 Asn Asn Gln Ser Thr Arg Trp Thr Thr Gly Gln Thr Gln Arg Asp Gly     450 455 460 Gln Thr Phe Glu Ile Asp Leu Ser Thr Val Gln Thr Phe Ser Arg Ile 465 470 475 480 Val Leu Asp Ser Ala Ala Ser Asp Asp Asp His Pro Arg Asn Tyr Glu                 485 490 495 Leu Tyr Val Ser Asn Asp Gly Ser Asn Trp Gly Ser Pro Val Ala Thr             500 505 510 Gly Ala Gly Asp Ser Ser Gly Val Thr Val Ile Asp Phe Pro Ser Val         515 520 525 Thr Ala Arg Tyr Val Leu Ile Ala Gln Ala Gly Ser Asp Ser Ser His     530 535 540 Trp Trp Ser Ile His Glu Leu Ser Ile Tyr Asn Val Gln 545 550 555 <210> 210 <211> 1674 <212> DNA <213> Microbulbifer degradans <400> 210 atgctacaca tctctgttca accaaatctt acccgctcta tttctgggtt cttgttgggc 60 ctagcgggcg ttgtctcggg ctcggcttac gcgcagtgga acccagcacc caactgggag 120 gacagctatt ctgtgaatgg agtttgctac tgtaattcca gtaattatga tcatgggcta 180 agcgccaaaa cagcacctac tcccattggc gagcttaacg ttgtcgatat atgtaccgac 240 atcaaagccg tgcttggtga gggggcaacg aatggacgta ttccatttaa cgacattcag 300 tgtggtaacg ggcccgccaa cgatgctgcc gatgaagcgg ggtgcccagg ccgagtggat 360 attggctcgg cagggtgtga tgtgattggg cctaaatggg atttggttag cgtttacggg 420 ccatggccag atggcgggct taatagggat gcttgggaag tgagtgcctc aaacggttct 480 ggtagcgcgc aactggcgct tgatggcttg gcgagtacgc gctgggcgac gggtgtgttt 540 caatcgccag gtcagtattt tgatattgat tttcaagacg cgctaacttt cgattcaata 600 gtacttgcta ccaccgagaa cccagaagat tacccaaggg cttacgaggt ttacatttca 660 tcaaatggca gcgactgggg agtaccagtg gtgacgggag ccggtaacgg ctccactaca 720 actatcgagc tagatactgt taccactcgc tacttaagaa ttttacaaac cggcagctca 780 tctaaccgtt ggtggtccat tcatgaattt aacatattca attctgggcc aattccgcca 840 gagccagagt acccggcatt agatagaagt gactggacag tttctgcatc ggtaaatggc 900 gcagaagccg aattcgctat cgactcttca cccaatacgc ggtgggatac agcacaaagc 960 caacgagcag ggcagagttt tgaggtagat ttaggagaac tcaacacgct agcggccatt 1020 gagctggatt cagcaggcag tgccaatgac tatccgcggg gttacgccgt ttatgtatcg 1080 aacgatggct caaactgggg gagcgctatc gcttctggcg ctgcagtaag cgcgtccaca 1140 acaatagaat ttactccagt aagcgctcgc ttcgttaaaa tagagcagac gggaggtgac 1200 ggacactatt ggtggtctat acacaactta aatatttttg gtgagccttc agataaccca 1260 caaccgacta tagagctact cgatagcact ccctggtctc tagccgcgaa caggcgaaat 1320 agcgcggctg gcaatgccat agataataac cagtctacac gatggaccac gggccagacc 1380 cagcgcgatg gtcagacgtt cgaaattgac ctgagcactg tgcaaacctt tagccgtatt 1440 gttttagatt ccgcagcgag tgacgatgat caccctcgca attatgaact ttatgtctct 1500 aacgatggtt ctaattgggg cagcccagtg gcaaccggtg caggagatag tagcggagta 1560 acggtaatag atttccccag cgtgaccgct cgctatgtac ttatagctca ggctggctcg 1620 gatagctcac actggtggtc cattcacgag ttgagtattt acaacgtaca ataa 1674 <210> 211 <211> 674 <212> PRT <213> Microbulbifer degradans <400> 211 Met Lys Ile Ile Arg Leu Leu Val Leu Cys Leu Gly Val Val Ser Ser 1 5 10 15 Val Val Ala Val Ala Ser Ser Ser Glu Pro His Asp Glu Gly Asn Ala             20 25 30 Leu Glu Ser Ala Leu Trp Asp Thr Leu Thr Ile Pro Val Cys Trp Glu         35 40 45 Asn Pro Glu Ala Phe Pro Val Glu Glu Gln Ala Trp Val Arg Lys Ala     50 55 60 Val Glu Arg Thr Trp Glu Gln Glu Ser Leu Val Arg Phe Thr Gly Trp 65 70 75 80 Gly Glu Cys Gly Ala Asn Asp Asp Gly Ile Arg Ile Leu Val Asp Asp                 85 90 95 Val Gly Pro His Val Lys Gln Leu Gly Ser Arg Leu Asp Gly Tyr Val             100 105 110 Asn Gly Met Val Leu Asn His Thr Phe Gln Asn Trp Gly Thr Ser Cys         115 120 125 Ser Tyr Arg Arg Gln Tyr Cys Ala Glu Val Ile Ala Val His Glu Phe     130 135 140 Gly His Ala Leu Gly Phe Ala His Glu Gln Asn Arg Asp Asp Thr Asp 145 150 155 160 Asp Met Cys Ala Ala Glu Ala Gln Gly Thr Asp Gly Asp Ile Tyr Val                 165 170 175 Gly Ala Trp Asp Leu Asp Ser Val Leu Asn Tyr Cys Asn Pro Glu Trp             180 185 190 Asn Gly Ala Gly Asn Leu Ser Asp Thr Asp Ile Glu Met Val Gln Leu         195 200 205 Phe Tyr Gly Gln Pro Ile Glu Thr Ser Gly Asn Glu Pro Val Ala Ile     210 215 220 Cys Ser Val Ser Ala Ser Ser Ser Asp Gly Asn Ile Ala Glu Asn Thr 225 230 235 240 Leu Asp Gly Asp Tyr Ala Thr Arg Trp Ser Ala Asn Gly Asp Gly Glu                 245 250 255 Trp Ile Gln Phe Asn Leu Cys Glu Ser Gln Val Ala Asp Arg Val Glu             260 265 270 Leu Ala Trp Tyr Lys Gly Asp Thr Arg Ser Ser Thr Phe Ser Ile Glu         275 280 285 Tyr Ile Thr Thr Asp Gly Tyr Val Trp Tyr Thr Thr Pro Val Arg Arg     290 295 300 Tyr Ser Ser Gly Ala Ser Leu Gly Leu Glu Ser Ala Ser Phe Thr Ala 305 310 315 320 Ala Glu Ile Gln Ser Leu Arg Ile Thr Gly Tyr Gly Asn Ser Ser Asn                 325 330 335 Thr Trp Asn Ser Ile Thr Glu Ala Val Ile Tyr Thr Pro Ser Ala Gly             340 345 350 Leu Asp Tyr Pro Asn Leu Val Ala Pro Thr Asp Val Ala Ala Thr Val         355 360 365 Thr Gly Gln Ser Gln Ile Thr Ile Ser Trp Ala Asp Thr Asn Ser Glu     370 375 380 Glu Glu Ser Tyr Phe Val Glu Ala Arg Ile Gly Gly Asn Asp Phe Phe 385 390 395 400 Ala Ile Gly Leu Thr Glu Ala Asn Ala Thr Ser Tyr Thr His Thr Asp                 405 410 415 Leu Ile Ala Glu Gly Leu Tyr Glu Tyr Arg Val Thr Ala Val Ser Gly             420 425 430 Ile Ile Arg Ser Asn Phe Ala Ala Ala Ser Val Tyr Phe Gln Pro Ala         435 440 445 Gly Gly Ser Gln Ala Asn Leu Val Arg Pro Glu Asn Phe Thr Val Thr     450 455 460 Ala Asn Ala Gln Gly Gln Ile Ala Leu Ser Trp Val Asp Val Ser Glu 465 470 475 480 Gly Glu Glu Gly Tyr Thr Leu Glu Tyr Lys Leu Ser Ser Glu Ala Glu                 485 490 495 Phe Thr Val Ile Glu Leu Ala Ala Asn Ala Asp Ser Ala Ile Val Ser             500 505 510 Asp Leu Ala Ala Gly Ser Tyr Tyr Phe Arg Val Ser Ser Tyr Phe Gly         515 520 525 Asn Asn Ala Ser Glu Tyr Ser Glu Leu Leu Val Thr Val Phe Ala Ser     530 535 540 Glu Ala Thr Ile Val Pro Val Asp Val Tyr Ala Ser Ser Asp Asp Gly 545 550 555 560 Asn Val Ala Ser Asn Val Phe Asp Asn Asp Tyr Ser Thr Arg Trp Ser                 565 570 575 Ala Phe Gly Val Gly Glu Asn Leu Thr Ile Ala Leu Gly Asp Asn Tyr             580 585 590 Leu Val Thr Asp Met Arg Ile Ala Trp Tyr Lys Gly Asp Gln Arg Gln         595 600 605 Thr Arg Phe Gln Val Glu Val Ser Asp Asp Asn Gln Thr Trp Val Gln     610 615 620 Val Phe Asp Gly Ile Asn Ser Gly Glu Ser Leu Ser Leu Glu Thr Thr 625 630 635 640 Phe Gly Gly Asp Tyr Arg Ala Ser Tyr Ile Arg Ile Ile Gly Leu Gly                 645 650 655 Asn Glu Phe Asn Asn Trp Asn Ser Ile Thr Glu Val Ser Ile Lys Gly             660 665 670 His leu          <210> 212 <211> 2025 <212> DNA <213> Microbulbifer degradans <400> 212 gtgaaaatca tacggttgct tgttttgtgt ttgggggtag taagttctgt ggttgcggta 60 gctagtagct cagagccaca tgatgaaggt aatgcattag agagtgcatt gtgggatacg 120 ttaactattc ctgtgtgctg ggagaatcca gaggcttttc ctgtagaaga gcaagcttgg 180 gtaagaaaag cggttgagcg tacttgggag caggagtcat tagtccgctt caccggttgg 240 ggcgagtgtg gggcgaacga tgatgggata cgtattttag tggatgatgt tgggccgcat 300 gttaagcagc taggctcgcg tttggatggc tatgttaatg gcatggtact aaaccataca 360 ttccaaaatt ggggcaccag ctgcagttac cgtcgtcaat attgtgcaga agtgattgca 420 gttcatgagt ttggtcatgc tctaggcttt gcccatgaac aaaacagaga tgacaccgat 480 gatatgtgcg cggccgaggc gcagggtact gacggcgaca tttatgtcgg cgcctgggac 540 ttggattcgg ttttaaacta ttgcaaccct gagtggaatg gcgcgggcaa tctaagcgat 600 accgatatag agatggttca gctgttttac ggtcagccca ttgaaacgtc tggtaacgag 660 cctgtagcta tttgtagtgt tagcgccagt tcaagcgatg gcaatattgc agaaaatacg 720 ctagacggcg attacgctac gcgttggtct gcaaacggcg acggcgagtg gatacaattt 780 aatttgtgtg aaagccaagt cgcggatcgt gtcgagttag cttggtataa gggagatact 840 cgctcaagta ccttctctat tgagtatata actaccgatg gttacgtttg gtataccacg 900 cctgttcgtc gatattcttc cggtgcttcg ctaggcttgg aatctgcgtc atttactgca 960 gctgaaattc agtccttgcg tattactggc tacggcaact cgtctaacac ttggaatagt 1020 attaccgaag cggtaattta tactccaagc gctgggctag attaccctaa cttagttgca 1080 cctacagatg tggcggctac ggtaacaggg caatcgcaaa ttactatttc atgggcagat 1140 actaatagcg aagaagaaag ctattttgtt gaggcgcgta ttggtggcaa cgattttttc 1200 gctataggtc ttactgaggc gaacgcaaca tcttatacgc ataccgacct tattgctgaa 1260 ggcctttacg agtatcgtgt tactgccgta agcggcatta ttcgctcaaa ctttgcagct 1320 gcaagtgtat attttcaacc tgcaggcggc tcgcaggcta atcttgtgcg cccagaaaac 1380 ttcactgtaa cagctaatgc gcaagggcaa atagcactaa gttgggtgga tgtaagtgaa 1440 ggcgaagagg gttatacctt agagtataag cttagtagtg aagctgaatt tactgtaatt 1500 gagctagctg cgaatgccga ttctgcaatt gttagcgatt tggctgcggg tagttactat 1560 tttcgtgtaa gcagttattt cggcaacaat gcttctgaat acagcgagct tttggttact 1620 gtgtttgcat cagaagcgac tattgtgccg gttgatgttt atgcatctag tgatgatggc 1680 aatgtggcaa gcaatgtttt tgataatgat tactctacgc gatggtcggc ttttggtgta 1740 ggtgagaatt taaccattgc gctgggggat aattatctcg ttaccgatat gcgcatagcg 1800 tggtacaaag gtgatcaacg tcaaacccgc tttcaggtag aggtgagtga tgataaccaa 1860 acctgggtgc aagtgtttga tgggattaac tcgggtgagt cgttaagttt agagacgacg 1920 tttggcggtg attatcgcgc aagctatatt cgaattatcg gtttgggtaa tgagtttaat 1980 aactggaaca gtattactga ggtaagcatt aaagggcacc tctag 2025 <210> 213 <211> 818 <212> PRT <213> Microbulbifer degradans <400> 213 Met Ile Lys Leu Ser His Leu Lys His Cys Arg Lys Phe Cys Ile Ser 1 5 10 15 Leu Leu Cys Ala Leu Gly Met Ala Asn Ala His Ala Ala Leu Asn Val             20 25 30 Thr Ala Ser Ala Asp Asp Gly Asn Val Pro Ala Asn Thr Leu Asp Asp         35 40 45 Asn Ile Asp Thr Arg Trp Ser Ala Asn Gly Ser Gly Gln Trp Ile Glu     50 55 60 Tyr Asp Leu Gly Ala Thr His Thr Val Asp Ala Val Gln Ile Ala Phe 65 70 75 80 Phe Arg Gly Asp Val Arg Asp Ala Thr Ile Asp Ile Gln Val Ser Asn                 85 90 95 Asp Gly Gly Asn Trp Gln Thr Leu Phe Ser Gly Thr Pro Pro Thr Arg             100 105 110 Thr Leu Ala Gln Gln His Phe Glu Leu Asp Asp Thr Ser Ala Arg Tyr         115 120 125 Val Arg Ile Val Gly Tyr Gly Asn Ser Gln Asn Asn Trp Asn Ser Ile     130 135 140 Thr Glu Phe Asp Val Val Thr Leu Ala Ser Gly Glu Asn Ile Ala Leu 145 150 155 160 Gly Lys Ala Thr Ser Gln Ser Ser Thr Gly Tyr Glu Gly Val Ser Ser                 165 170 175 Arg Ala Val Asp Gly Asn Thr Asn Gly Asn Trp Asn Gln Gly Ser Ile             180 185 190 Thr His Thr Asn Asn Glu Tyr Gln Pro Trp Trp Gln Val Asp Leu Gly         195 200 205 Ser Val Arg Ser Ile Asp Gln Val Asn Leu Trp Asn Arg Thr Asn Cys     210 215 220 Cys Ser Ser Arg Leu Ser Ala Phe Tyr Val Leu Val Ser Asp Val Pro 225 230 235 240 Phe Thr Ser Gln Thr Leu Ser Gly Ala Leu Ser Gln Ala Gly Val Ser                 245 250 255 Ala Tyr Tyr Phe Asn Asp Thr Ala Gly Ser Pro Thr Glu Ile Asn Ile             260 265 270 Asp Arg Thr Gly Arg Tyr Val Arg Val Gln Leu Ser Gly Thr Asn Pro         275 280 285 Leu Ser Leu Ala Glu Val Glu Val Ile Glu Gly Ser Glu Ile Val Pro     290 295 300 Pro Ala Pro Thr Gly Pro Asp Ala Ser Trp Thr Tyr Cys Ala Ala Glu 305 310 315 320 Arg Glu Gln Cys Ala Phe Ser Asn Ile Lys Glu Val Ala Tyr Gly Ala                 325 330 335 Gly Asp Ser Trp Asn Tyr Ser Val Glu Leu Asp Gly Val Thr Cys Asn             340 345 350 Asn Thr Asn Leu Gly Asp Pro Val Arg Gly Thr Val Lys Ser Cys Trp         355 360 365 Val Arg Asn Ala Gln Gln Asn Tyr Val Ala Val Arg Asn Leu Ala Glu     370 375 380 Leu Gln Asn Ala Ile Ser Asn Ser Asn Gln His Ile Arg Met Lys Arg 385 390 395 400 Gly Val Tyr Glu Ala Thr Ala Leu Met Ser Asp Asn Thr Thr Val Phe                 405 410 415 Arg Phe Asp Gly Ala Asn Asn Val Leu Asp Phe Thr Gly Val Thr Ile             420 425 430 Gln Val Pro Thr Lys Leu Leu Asn Ser Met Ser Thr Gln Pro Ile His         435 440 445 Ser Gln Val Thr Tyr Asp Val Met Gly Asp Asn Ile Thr Phe Leu Asn     450 455 460 Gly Thr Phe Glu Asn Thr Tyr Pro Asn Gly Gln His Asp Val Thr Asp 465 470 475 480 Phe Thr Ala His Asn Lys Asn Pro Asp Tyr Trp Pro Ala Arg Gln Met                 485 490 495 Thr Glu Phe Arg Val Trp Gly Asn Gly Val Gln Phe Leu Asn Asn Thr             500 505 510 Ile Thr Val Arg Gly Ser Tyr Pro Tyr Gly Tyr Gly Asp Met Leu Gly         515 520 525 Lys Gly Ala Gly Ser Ala Val Tyr Leu Arg Lys His Ala Gly Val Gln     530 535 540 Ile Ser Gly Asp Asn Val Leu Ile Asp Gly Met Lys Leu Thr Val Leu 545 550 555 560 Ala Phe Gly His Gly Ile Phe Met Gln Gly Ala Asp Asn Thr Val Ile                 565 570 575 Lys Asn Ser Val Val Gln Gly Arg Met Arg Leu Gly Ala Asp Met Tyr             580 585 590 Asn Asp Gly Pro Asp Ser Leu Met Gly Pro Phe Asn Phe Glu Gln Gln         595 600 605 Tyr Pro Asp His Phe Val Gly Leu Pro Ile Val Arg Asp Leu Met Tyr     610 615 620 Asn Leu Thr Glu Asp Gly Ile Arg Ala Tyr Thr Gln Gly Thr Lys Leu 625 630 635 640 Asp Gly Ser Val Val Arg Thr Gly Ala Ile Thr Val Glu Asp Thr Lys                 645 650 655 Val Ile Asn Met Arg Gly Cys Ile Thr Thr Pro Leu Ala Ser Lys Pro             660 665 670 Ser Tyr Ile Lys Asn Val Glu Ile Gln Gly Cys Ser Val Gly Tyr Ala         675 680 685 Leu Ala Asn Asn Ser Asp Val Ile Asn Ser Arg Gly Asp Ala Gly Tyr     690 695 700 Gly Pro Leu Leu His Ser Thr Tyr Asp Thr Arg Asn Asn Ala Asn Val 705 710 715 720 Glu Ile Thr Val Thr Asn Ile Pro Ser Thr Gly Ser His Ala Phe Ala                 725 730 735 Tyr Ile Ala Gly Ser Gly His Asn Ile Thr Phe Leu Ser Asp Gly Ser             740 745 750 Asn Pro Asp Val Pro Arg Glu Ile Arg Val Gly Asp Thr Gly Asp Arg         755 760 765 Trp Ala Gly Asp Pro Ser Tyr Gln Asp Ala Ala Asn Ile Leu Leu Ile     770 775 780 Asn Glu Thr Asn Gln Pro Val Val Leu Thr Glu Thr Ser Arg Asn Ile 785 790 795 800 Thr Gly Glu Ser Val Gly Glu Val Thr Asp Asn Gly Thr Gly Asn Ser                 805 810 815 Leu lys          <210> 214 <211> 2457 <212> DNA <213> Microbulbifer degradans <400> 214 atgataaaac tatctcacct caaacactgc aggaaatttt gtatttcttt gctttgcgcg 60 ctgggcatgg ctaatgctca cgccgcatta aatgtaactg catctgcgga tgacggcaat 120 gtgcccgcga ataccttaga tgacaatata gatacgcgct ggtcggcgaa tggatctggg 180 cagtggattg aatatgattt gggcgcgaca cacactgtgg atgctgtgca aatagcattt 240 tttcgcggag atgtgcgcga tgcaaccatc gacattcaag tgtcgaacga tggcggcaat 300 tggcaaacac ttttttcggg tacgccacca acccgaacct tagcgcagca acattttgag 360 ttggatgata cttctgcgcg ctatgtgcgt attgtaggtt atggtaacag ccaaaataat 420 tggaacagta ttaccgagtt cgatgtggtt acgcttgcaa gtggagaaaa tattgctctt 480 ggtaaagcta catcccaatc atccactggc tatgagggtg tatctagccg tgcggtagat 540 ggcaacacca atggtaactg gaatcaaggc tcgattaccc acaccaataa cgagtatcaa 600 ccttggtggc aagtggatct aggctcagtt agatctatcg accaagttaa cttgtggaat 660 cgtaccaact gctgcagctc gcgtttatcg gcgttttatg tgttggtgtc cgatgtgccc 720 tttacatcgc aaaccttaag cggtgcgctt agtcaagcgg gtgtaagtgc ttattatttt 780 aatgatactg cgggcagccc aaccgaaata aatatagatc gcacaggtcg ctatgtgcgt 840 gtacaacttt ctggcacaaa cccattaagc ctagcggaag ttgaagttat tgaaggcagc 900 gaaattgttc caccagcacc aaccggccca gatgcatcat ggacttattg tgccgccgag 960 cgcgagcaat gtgcattttc taatataaaa gaagtggcct acggcgctgg cgatagctgg 1020 aactactctg tcgagctaga cggcgtaact tgtaataata ctaacttggg tgaccccgtt 1080 cgcggcacgg ttaaatcgtg ctgggtacgc aacgcacaac aaaattatgt ggcggtgcga 1140 aaccttgctg aattgcaaaa tgctatttcc aatagcaatc aacacattcg catgaagcgc 1200 ggtgtgtatg aagctacagc gttaatgtct gataacacca ccgtatttcg attcgacggt 1260 gcgaataatg tattggattt taccggtgta accattcagg tacccactaa actcttaaac 1320 agtatgagta cccagcctat tcactcgcaa gtaacctacg atgtgatggg cgataacatt 1380 acttttttaa acggtacttt cgaaaatacc tatccgaatg gccagcacga tgtaacagat 1440 tttaccgccc ataataaaaa cccagactat tggcccgcgc gtcaaatgac ggagtttaga 1500 gtgtggggga acggcgtaca gtttttaaat aacaccatta ctgtgcgcgg ttcatacccg 1560 tatggctacg gtgatatgtt aggtaagggc gccggttctg cagtgtattt acgcaaacat 1620 gctggtgtac aaatttctgg cgacaatgta ttaattgacg gcatgaaatt aaccgtactt 1680 gcattcggcc acggcatttt tatgcagggt gcagataaca cagtaattaa aaactctgtt 1740 gtacaagggc gtatgcgctt aggcgccgat atgtataacg acgggccaga ctccttaatg 1800 gggccgttta attttgagca gcaataccca gatcactttg tgggcctgcc aattgtgcgc 1860 gacctaatgt acaaccttac cgaagatggc atacgcgctt atacacaagg caccaaatta 1920 gatggtagtg tggtgcgtac tggtgcaatt actgtagaag acaccaaagt aattaatatg 1980 cgcggttgta ttactacgcc attggcttct aagccaagtt atataaaaaa tgtagaaatt 2040 caaggttgta gtgtgggtta cgcgctggcg aataatagcg atgtaatcaa ttcgcgcggt 2100 gatgcaggct acggtccgct attgcactct acgtacgata cgcgcaacaa cgccaatgtc 2160 gaaataaccg ttaccaatat tccatctacc ggctcacacg cttttgcgta tattgcaggc 2220 tctggtcata acattacctt cctaagtgat ggcagcaacc cagatgtgcc gcgcgaaata 2280 cgcgtaggcg atactggcga ccgctgggct ggcgacccaa gctatcaaga tgcagcaaat 2340 atattactca taaacgaaac caaccaaccg gtggtgttaa ctgaaaccag tagaaatatt 2400 actggtgaaa gtgtgggtga ggtgactgat aatgggacgg gaaatagctt gaaatag 2457  

Claims (23)

A process for preparing ethanol from lignocellulosic material, A process for preparing ethanol comprising treating lignocellulosic material with one or more effective saccharifying amounts of the compounds listed in FIGS. 4-11 to obtain saccharides and converting the sugars to produce ethanol. The method of claim 1, wherein at least one of the compounds listed in FIGS. 4-11 is from Microbulbifer degradans 2-40. The method of claim 1, wherein the lignocellulosic material is treated with an effective glycosylation amount of all the compounds listed in FIGS. 4-11. The method of claim 1, wherein at least one of the compounds listed in FIGS. 4-11 is in dry form, in a buffer, or in the form of a paste, paint, or micelle. The method of claim 1, wherein at least one of the compounds listed in FIGS. 4-11 is in a system essentially comprising one or more of the compounds listed in FIGS. 4-11. The method of claim 1, wherein at least one of the compounds listed in FIGS. 4-11 is present in a system further comprising a metal ion, a chelator, a detergent, an organic ion, an inorganic ion, or one or more additional proteins. Method for producing ethanol. The method of claim 6, wherein the one or more additional proteins are biotin and / or albumin. The method of claim 1, wherein the treatment is performed in an oceanic environment. The method of claim 8, wherein the treatment is performed in water. The method of claim 1, wherein the one or more compounds listed in FIGS. 4-11 is the cellulase cel5A listed in FIG. 4. A process for preparing ethanol from lignocellulosic material, A method of making ethanol, comprising contacting lignocellulosic material with a microorganism that expresses one or more effective glycosylation amounts of the compounds listed in FIGS. 4-11 to obtain a sugar and converting the sugar to produce ethanol. The method of claim 11, wherein the microorganism is Microbulbifer degradans 2-40. The recombinant microorganism of claim 11, wherein the microorganism is a recombinant microorganism comprising a chimeric gene comprising at least one polynucleotide encoding a polypeptide comprising at least one amino acid sequence of the compounds listed in FIGS. 4-11; Wherein said gene is operably linked to a regulatory sequence that enables expression of said amino acid sequence by said microorganism. The method of claim 13, wherein the recombinant microorganism is a bacterium or yeast. The method of claim 13, wherein the recombinant microorganism is Escherichia coli . The method of claim 14, wherein the bacterium or yeast is ethanologenic. The method of claim 11, wherein the contacting is performed in a marine environment. The method of claim 17, wherein the contacting is performed in water. The method of claim 11, wherein at least one of the compounds listed in FIGS. 4-11 is the cellulase cel5A listed in FIG. 4. A process for preparing ethanol from lignocellulosic material, A method of making ethanol, comprising contacting lignocellulosic material with an ethanologenic microorganism expressing at least one effective glycosylation amount of the compounds listed in FIGS. 4-11 to produce ethanol. Use of one or more of the compounds listed in FIGS. 4-11 for the production of ethanol from lignocellulosic material. Use of a microorganism expressing at least one effective glycosylation amount of the compounds listed in FIGS. 4-11 for the production of ethanol from lignocellulosic material. Use of an ethanologenic microorganism expressing at least one effective glycosylation amount of the compounds listed in FIGS. 4-11 for the production of ethanol from lignocellulosic material.

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