KR20090086807A - Method for producing processed ginseng or processed ginseng extract - Google Patents

Abstract

A processed ginseng or processed ginseng extract is provided to remarkably increase the content of Rg2, Rg3, and Rb1 in ginsenoside. A processed ginseng or processed ginseng extract having increased ginsenoside content by hydrolysis is produced using ginseng with enzyme. The enzyme is beta-glucanase, pectinase, hemicellulase, cellulase, arabinase, or xylanase. The ginseng is a red ginseng. The ginsenoside is Rb1, Rg2, and Rg3.

Description

Method for producing ginseng or processed ginseng extract {Method for producing processed ginseng or processed ginseng extract}

The present invention relates to a process for preparing processed ginseng or processed ginseng extract. More specifically, the present invention relates to a process for preparing processed ginseng or processed ginseng extract with an increased content of ginsenosides. More specifically, the present invention relates to a process for producing processed ginseng or processed ginseng extract with increased content of ginsenosides using hydrolysis by enzymes.

Ginseng is the broadest concept that includes ginseng, white ginseng, red ginseng, wild ginseng, camphor, rice ginseng, and original ginseng. Ginseng is a natural product that has been used as a very valuable medicine in oriental medicine, such as China. Many documents contain the effects of ginseng. However, despite the long history of use, scientific identification through clinical research is insufficient. Nevertheless, even though modern medicine has been introduced, the popularity of ginseng as a medicine is not falling, and it is the first place among the health functional foods. .

The main functional ingredient of ginseng is ginseng saponin called "ginsenoside" which is specially named only ginseng saponin among various saponins of plants.

Scientific research on ginseng components began after the 1960s. The chemical structure of saponins is a glycoside that consists of sugar and aglycone. When these saponins are absorbed by the body, they are broken down by the unique microorganisms in the human intestine and absorbed into the body. However, the intestinal microorganisms that break down ginseng saponins differ depending on the human constitution and dietary habits, and their presence and degree of retention. This can cause differences in efficacy after taking ginseng. Some people only absorb the diol form of saponins, some people only absorb the triol form, and some people do not absorb ginseng saponins at all.

The difference between white ginseng and red ginseng is the difference in the content and content of ginsenosides contained. White ginseng mainly contains ginsenosides Ra, Rb1, Rb2, Rc, Rg1, Re and the like. The red ginseng forms a physicochemical converter in the process of steaming the components in the white ginseng. For example, a large amount of ginsenoside Rg3 is produced in the steaming process. In addition, red ginseng contains small amounts of ginsenosides Rh2 and Rh1. These ingredients have different biological activities. White ginseng contains high molecular weight polysaccharides, but acidic polysaccharides are lower than red ginseng. Red ginseng contains a large amount of polysaccharides whose molecular weight is smaller than that of white ginseng.

The main components of ginseng are ginsenosides, which are saponins, and ginsenosides Rb1, Rb2, and Rc, which are protopanaxadiols, and ginsenosides Re, Rg1, and Rf, which are protopanaxatriols, are known. Representative pharmacological actions of these components include anti-cancer activity, anti-inflammatory, anti-diabetic action. With these components, using cancer cells directly to measure anti-cancer activity in vitro ( in vitro ), there is no activity. However, when these components are administered orally, they are metabolized by enterobacteriaceae and converted to compounds such as compound K, which shows strong cancer cell toxicity and cancer metastasis inhibitory activity.

When ginsenosides are metabolized by the intestinal bacteria described above, they are as follows. First, ginsenosides Rb1, Rb2, and Rc, which are protopanaxadiol-based compounds, are metabolized into compound K via ginsenoside F2. This metabolic reaction is catalyzed by strains of the genus Bacteroides , Fusobacterium genus, Provetella genus, and the like. In addition, ginsenosides Re, Rg1, Rf, etc., which are protopanaxatriol-based compounds, are metabolized to ginsenosides Rh1 or F1 by the strains, and further metabolized to protopanaxatriol.

The components of ginseng can also be converted by physicochemical methods. For example, ginsenosides Rb1, Rb2, Rc, etc. in ginseng may be converted to ginsenoside Rg3 by heat treatment. Taking ginseng containing the converted ginseng saponin can be converted to ginsenoside Rh2 by intestinal bacteria in the intestine and further converted to protopanxadiol.

The difference between the ingredients is closely related to the difference in ginseng drug efficacy. If ginsenosides Rb1, Rb2, Rc containing a lot of white ginseng compound K can be transferred to the blood. On the other hand, in the case of red ginseng with a lot of ginsenoside Rg3, ginsenoside Rh2 can be transferred to the blood. In addition to other ginseng saponins may also be different in the transition to the blood, so in evaluating the efficacy of ginseng should be considered as above.

An object of one embodiment of the present invention is to change the ginsenoside composition of ginseng.

Another object of the present invention is to increase the content of Rg2 and Rg3 in ginsenosides of ginseng.

Another object of an embodiment of the present invention is to provide a method for effectively extracting the active ingredients in ginseng.

Another object of an embodiment of the present invention is to provide a ginseng extract with a high ginsenoside content.

Another object of an embodiment of the present invention is to provide a ginseng extract having a high content of Rg2 and Rg3 among ginsenosides.

Process for preparing processed ginseng or processed ginseng extract according to an embodiment of the present invention, ginseng, beta-glucanase (β-glucanase), pectinase (pectinase), hemicellulase (hemicellulase), cellulase (cellulase) ), Arabinase and xylanase to increase the content of ginsenosides by adding one or more enzymes selected from the group consisting of hydrolysis of ginseng.

Health food composition according to an embodiment of the present invention, ginseng, beta-glucanase (β-glucanase), pectinase (pectinase), hemicellulase (hemicellulase), cellulase (cellulase), arabinase (arabinase) ) And a hydrolyzate obtained by adding one or more enzymes selected from the group consisting of xylanase as an active ingredient.

By using the method of manufacturing processed ginseng or processed ginseng extract according to an embodiment of the present invention, ginseng or ginseng extract with a significantly increased content of ginsenosides can be obtained.

By using the method for preparing a processed ginseng or processed ginseng extract according to another embodiment of the present invention, a processed ginseng or processed ginseng extract having a high content of Rg2, Rg3 and Rb1 in ginsenosides can be obtained.

In one embodiment of the present invention, cellulase may be used as an enzyme for hydrolyzing ginseng. Examples of cellulase include cellulase derived from the genus Tricholderma . Cellulase derived from the genus Tricholderma includes Econase CE (AB Enzymes GmbH, Darmstadt, Germany). The optimum pH of Econase CE is 4.0 to 5.5 and the optimum temperature is 55 ° C.

Other enzymes with cellulase activity include Rapidase (DSM, Delft, Netherlands) and Viscozyme (Novozymes, Dittingen, Switzerland). Can be mentioned. Rapidase Asph. Asp. Niger or T. An enzyme derived from T. longibrachiatum , which has pectinase and hemicellulase activity in addition to cellulase activity. The optimum pH of Rapidase is 4.0 to 5.0 and the optimum temperature is 10 to 55 ° C. Viscozyme is an enzyme derived from the genus Asp. , Which contains not only cellulase activity but also arabinase, beta-glucanase, hemicellulase, and xylanase. Has activity.

In another embodiment of the present invention, pectinase may be used as an enzyme for hydrolyzing ginseng. As an example of pectinase, Cytolase PCL 5 (Gist-Brocades, Seclin, France) is mentioned. Cytolase PCL 5 is a pectinase derived from Aspergillus Niger , with an optimum pH of 2.5 to 5.0 and an optimal temperature of 10 to 55 ° C. Rapidase mentioned above also has the activity of pectinase and thus can be used as pectinase for hydrolyzing ginseng in this embodiment.

In another embodiment of the present invention, hemicellulose may be used as an enzyme for hydrolyzing ginseng. As hemicellulose, the above-mentioned Rapidase or Viscozyme can be used.

In another embodiment of the present invention, beta-glucanase may be used as an enzyme for hydrolyzing ginseng. An example of beta-glucanase is Ultraflo L (Novozymes, Dittingen, Switzerland). Ultraflo L is an enzyme derived from Humicola insolens with an optimal pH of 6 and an optimum temperature of 40 ° C. Since Viscozyme mentioned above also has beta-glucanase activity, Viscozyme may be used instead of Ultraflo L.

Other enzymes that may be used in another embodiment of the present invention include arabinase, xylanase, and the like.

 Ginsenoside Rg2 is ginsenoside which does not exist in white ginseng but exists only in red ginseng. Rg2 inhibits platelet aggregation, inhibits acetylcholine induced catecholamine secretion, inhibits intracellular calcium influx, antithrombin, memory decay, and smooth muscle cell proliferation. Hydrolysis by treating ginseng with pectinase can significantly increase the content of Rg2. In addition, the treatment of ginseng with cellulose or hemicellulose can also increase the Rg2 content to significantly higher levels.

Ginsenoside Rg3 also does not exist in white ginseng, but only in small amounts in red ginseng. Rg3 inhibits cancer cell metastasis, inhibits platelet aggregation and antithrombosis, inhibits liver injury, vascular relaxation, inhibits resistance of anticancer drugs, and protects nerves. When ginseng is hydrolyzed by treating pectinase, the content of Rg3 can be significantly increased. In addition, the treatment of beta-glucanase, cellulose or hemicellulose can increase the content of Rg3 to significantly higher levels.

Ginsenoside Rb 1 is contained in large amounts in both white and red ginseng. Rb1 is responsible for central inhibition and mental stabilization, aggressive behavioral suppression, analgesic, anticonvulsant, hypercholesterolemic, anti-anxiety, cholesterol biosynthesis, DNA, RNA, protein and lipid synthesis, protein synthesis Promotes action, promotes neuronal survival, promotes acylcholine release, improves memory, inhibits platelet aggregation, inhibits lipid peroxidation, vasodilation, promotes cholesterol metabolism, anti-inflammatory, and protects liver injury. Ginsenoside Rb1 can increase its content to significant levels when hydrolyzed by treatment with pectinase, cellulose, hemicellulose.

In addition, ginsenosides such as Rg1, Rf, Re, Rd, Rc, Rb2 may also be selected from beta-glucanize, pectinase, hemicellulose, cellulose and / or arabinase and xylase in ginseng. By treating the enzyme with hydrolysis, its content can be significantly increased.

 In the preparation method according to an embodiment of the present invention, the hydrolysis process by enzyme treatment may be performed simultaneously with the extraction and / or concentration process.

In a manufacturing method according to another embodiment of the present invention, the hydrolysis process by enzyme treatment may be performed separately before the extraction process.

The health food composition according to one embodiment of the present invention may include ginseng or extract thereof prepared by any one of the above-mentioned methods as an active ingredient.

Health food composition according to another embodiment of the present invention is characterized in that it further comprises gamma cyclodextrin. Although ginseng has excellent efficacy, its unique bitter taste is recognized as a big problem. Ginseng-induced bitter taste in the cavity of gamma cyclodextrin can significantly reduce the bitter taste of ginseng. When the amount of gamma cyclodextrin is 100 parts by weight of liquid ginseng extract, specifically, ginseng concentrate, the content of gamma cyclodextrin is preferably 5 to 20 parts by weight. If the amount of gamma cyclodextrin is less than 5 parts by weight, the bitter taste masking effect is insignificant, and if it exceeds 20 parts by weight, the formulation stability may worsen. More preferred gamma cyclodextrin content ranges from 7 to 10 parts by weight.

Hereinafter, the present invention will be described in more detail with reference to one embodiment, but the scope of the present invention is not limited thereto.

Example

<Example 1 to Example 5: Preparation of red ginseng hydrolyzate>

To 6 g of red ginseng powder purchased from Geumsan, 30 mL of phosphate buffer solution (50 mM, pH 5.0) was added and suspended, and 200 μL of each enzyme shown in Table 1 was added thereto, followed by hydrolysis at 45 ° C. for 12 hours. 300 mL of 80% ethanol was added to the hydrolyzate to reflux for 3 hours to extract ginsenosides, and the reflux was concentrated to 30 mL. Concentrates were prepared in the same manner as described above, except that the enzyme treatment was not performed in order to compare with the results of the examples (Comparative Example 1).

Example enzyme Main active Source Optimum pH Temperature (℃) Example 1 Ultraflo L (Novozymes, Dittingen, Switzerland) Beta-glucanize Hyumi coke insole lens (H umicola insolens) 6 40 Example 2 Rapidase (DSM, Delft, Netherlands) Pectinase, hemicellulose, cellulase Ah soup nigeo (Asp. Niger) & tea. Ronggi beuraki Atum (T. longibrachiatum) 4.0-5.0 10-55 Example 3 Viscozyme (Novozymes, Dittingen, Switzerland) Arabica, Cellulose, Glucanase, Hemicellulase, Xylanase Ah soup. (Asp.) In 3.3-5.5 40-50 Example 4 Cytolase PCL 5 (Gist-Brocades, Seclin, France) Pectinase Asph. Nigeo (Asp. Niger) 2.5-5.0 10-55 Example 5 Econase CE (AB Enzymes GmbH, Darmstadt, Germany) Cellulase Trichoder ma in the genus 4.0-5.5 55

Experimental Example 1 Measurement of Polyphenol, Total Sugar, Acid Sugar and Dry Matter Content

For the concentrate prepared according to Comparative Example 1, Examples 1 to 5, the amount of polyphenol, total sugar, acidic sugar and dry matter were measured.

Polyphenol content was determined by the Folin-Denis method (Dewanto, V., Wu, X., & Liu, RH (2002b) .Processed sweet corn has higher antioxidant activity.Journal of Agricultural and Food Chemistry, 50, 4959 -4964.).

The contents of total sugar and acid sugar were measured by phenol-sulfuric acid method and menBlumenkrantz and Asboe-Hansen method (Blumenkrantz, N. and Asboe-Hansen, G. (1973) Anal. Biochem., 54, 484). . The results are shown in Table 2 below.

division enzyme Total sugar (mg / ml) Acidic sugar (ug / ml) Polyphenols (µg / ml) Solid (%) Comparative Example 1 control 284.8 ± 30.4 ^d 160.00 ± 66.55 ^d 479.28 ± 10.80 ^c 8.47 ± 0.01 ^e Example 1 Ultraflo L 264.5 ± 31.9 ^d 377.67 ± 108.15 ^d 423.84 ± 24.24 ^d 7.85 ± 0.20 ^f Example 2 Rapidase 462.9 ± 37.4 ^ab 2124.71 ± 129.41 ^b 798.22 ± 12.56 ^a 12.77 ± 0.19 ^a Example 3 Viscozyme 516.5 ± 6.0 ^a 2626.67 ± 170.62 ^a 694.71 ± 2.78 ^b 11.63 ± 0.12 ^c Example 4 Cytolase PCL 5 393.1 ± 65.7 ^c 1167.84 ± 288.26 ^c 728.39 ± 63.86 ^b 12.15 ± 0.11 ^b Example 5 Econase CE 417.9 ± 26.6 ^bc 854.12 ± 216.30 ^c 114.73 ± 14.62 ^e 9.78 ± 0.16 ^d

As shown in Table 2, it was confirmed that the total sugars and acidic sugars of the hydrolyzate formed by the enzyme treatment increased significantly compared to Comparative Example 1 without the enzyme treatment. The content of polyphenols present in plants and showing various activities was high except for Example 5. Dry matter was also high in hydrolyzate.

Experimental Example 2: Determination of Ginsenoside Content

1 g of each concentrate prepared according to Comparative Example 1 and Examples 1 to 5 was weighed out, and 50 mL of 70% ethanol was added thereto, extracted twice at 80 ° C shaking incubator, and filtered using Whatman # 1 paper. The filtrate was dried under reduced pressure using a vacuum concentrator and dissolved by adding 50 mL of distilled water. 2 mL of diethyl ether was added and mixed with 1 mL of the solution, followed by centrifugation at 1500 rpm for 10 minutes to remove diethyl ether, and then, 1.5 mL of water-saturated butanol was added thereto, mixed, and the butanol layer was recovered. 1.5 ml of distilled water was added to the butanol layer, which was recovered by repetition three times, mixed and centrifuged to remove the water layer. The procedure was repeated twice to wash water-soluble impurities. The butanol layer thus obtained was dried by injecting N ₂ gas at 40 ° C., and 0.5 mL of methanol was added thereto, followed by filtration through a 0.45 μm membrane filter, which was used as a sample for HPLC analysis. HPLC analysis was performed by ELSD using HPLC equipped with Prevail Carbohydrate ES 5μ column (Alltech, USA) and performed under the conditions as indicated in Tables 3 and 4 (Gradient Table) below. On the other hand, ginsenoside analysis includes 14 standard materials (compound K, Rh2, Rh1, Rg5, Rk1, Rg2, Rg3, Rg1, Rf, Re, Rd, Rb2, Rc, A standard curve was prepared using Rb2) and the content was calculated from each peak area ratio.

Devices SP930D Solvent delivery pump SDV50A Vacuum degasser and valve module CTS 30 Column oven (Young-Lin Co. Ltd., Korea) Detector ELSD (GmbH, Germany) column Prevail Carbohydrate ES 5u, 250 × 4.6 mm (Alltech, USA) Oven temperature 35 ℃ menstruum A (Acetonitrile: Water: IPA = 80: 5: 15) B (Acetonitrile: Water: IPA = 67: 21: 12) Injection volume 20 μl

Time (min) Flow (ml / min) % A % B Initial 0.8 90 10 28 0.8 15 85 35 0.8 20 80 45 0.8 25 75 50 0.8 10 90 51 0.8 0 100 57 0.8 75 25 58 0.8 90 10 65 0.8 90 10

The results are shown in Table 5 below.

Ginsenoside concentration (㎍ / mL) Comparative Example 1 (control) Example 1 (Ultraflo L) Example 2 (Rapidase) Example 3 (Viscozyme) Example 4 Cytolase Example 5 (Econitase) Rh2 Rh1 Rg2 292.92 123.15 1027.34 374.33 1444.25 516.83 Rg3 49.34 222.31 182.21 132.55 271.09 238.85 Rg1 2369.73 103.55 5087.37 2863.61 5528.22 3372.65 Rf 492.43 142.07 1257.42 858.56 1125.46 1373.97 Re 4514.99 116.61 9779.11 7355.22 7612.66 11388.15 Rd 3472.62 158.24 6040.91 4762.92 20347.8 3787.97 Rc + Rb2 2729.64 192.35 20572.44 3726.69 26762.88 12206.30 Rb1 4889.36 129.62 10209.58 8150.15 10074.82 10669.09

As shown in Table 5, the content of Rg2 having high anticancer effect was 1027.34 μg / mL and 1444.25 μg / mL in the hydrolyzate of Example 2 (rapidase) and Example 4, respectively, of Comparative Example 1 (cotrol). The content was higher than 292.92 μg / mL. In addition, the content of Rg3 was 49.34 μg / mL in Comparative Example 1 (control), while all the hydrolyzate showed a high content of 132.55-271.09 μg / mL. The content of Rb1 was 4889.36 μg / mL in Comparative Example 1 (control), while the hydrolyzate except Example 1 (Ultraflo L) showed a high content of 8150.15-10669.09 μg / mL. In the case of white ginseng containing a lot of ginsenosides Rb1, Rb2 and Rc, compound K can be transferred to the blood.

<Example 6 to Example 9: Preparation of red ginseng hydrolyzate by various enzyme combinations 1>

The concentrate was obtained in the same manner as in Examples 1 to 5, except that Rapidase, Cytolase, and Viscozyme, which were excellent in ginsenosides production, were used in various combinations of the hydrolyzing enzymes used in Examples 1 to 5.

Experimental Example 3 Measurement of Total Sugar, Acid Sugar, and Dry Matter 2

For each of the concentrates obtained according to Examples 6 to 9, the total sugar, acid sugar, dry weight were measured under the same conditions as in Experimental Example 1. The results are shown in the table below.

sample enzyme Enzyme addition amount (μl) Total sugar (mg / ml) Acidic sugars (㎍ / ml) Polyphenols (µg / ml) Building volume (%) Comparative Example 2 control 0 138.83 ± 8.34 ^c 152.16 ± 41.32 ^b 341.35 ± 7.04 ^c 9.05 ± 0.18 ^c Example 6 Rap + Visco + Cytol 100 + 100 + 100 223.36 ± 68.71 ^b 2646.28 ± 238.02 ^a 561.33 ± 32.60 ^ab 14.83 ± 0.24 ^a Example 7 Rap + Visco 150 + 150 210.98 ± 15.06 ^b 2575.69 ± 255.69 ^a 591.30 ± 18.35 ^a 15.07 ± 0.49 ^a Example 8 Rap + Cytol 150 + 150 199.31 ± 28.37 ^b 2507.06 ± 123.95 ^a 518.32 ± 30.49 ^b 14.33 ± 0.42 ^b Example 9 Visco + Cytol 150 + 150 228.60 ± 4.76 ^a 2540.39 ± 207.25 ^a 583.89 ± 27.50 ^a 14.67 ± 0.46 ^ab

As shown in Table 6, the total sugar content showed the highest content of 228.60 mg / ml when Viscozyme and Cytolase were mixed (Example 9), and the acidic sugar content was obtained by mixing enzymes compared to Comparative Example 2. The content of degradant was 2507.06-2646.28 ㎍ / ml. The polyphenol contents were 591.30 and 583.89 ㎍ / ml of hydrolyzate obtained by mixing Rapidase + Viscozyme (Example 7) and Viscozyme + Cytolase (Example 9), respectively.

Experimental Example 4 Determination of Ginsenoside Content 2

For each concentrate obtained according to Examples 6 to 9, the change of ginsenoside content was measured under the same conditions as in Experimental Example 2. The results are shown in the table below.

Fraction Concentration (ppm) (/ mL) Comparative Example 2 (control) Example 6 (Rap + Visco + Cytol) Example 7 (Rap + Visco) Example 8 (Rap + Cytol) Example 9 (Visco + Cytol) Rh2 0.0 89.3 0.0 0.0 0.0 Rh1 99.1 95.6 0.0 0.0 0.0 Rg2 292.9 372.7 334.0 200.7 348.9 Rg3 49.34 106.7 106.4 1014.6 104.5 Rg1 2369.73 2078.5 1654.5 0.0 1799.4 Rf 492.43 855.5 833.0 440.3 896.3 Re 4514.99 4506.9 4102.1 2522.8 5792.9 Rd 3472.62 5645.3 4087.6 3677.1 7747.8 Rc 5708.8 5029.8 4649.4 2881.5 6404.8 Rb2 3100.2 2736.1 2532.1 1584.0 3473.4 Rb1 4889.4 10566.5 9343.8 6583.7 12636.1

As shown in Table 7, Rg2 content was the highest when using Rapidase + Viscozyme + Cytolase mixture (Example 6), and Rg3 content was the highest when using Rapidase + Cytolase mixture (Example 8). In addition, the content of Rb1 was the highest when using Viscozyme + Cytolase mixture (Example 9).

<Examples 10 to 16>

Concentrates were prepared in the same manner as in Examples 1 to 5 except that hydrolysis was performed by various combinations of Rapidase, Viscozyme, Cytolase, and Econase.

Experimental Example 5 Measurement of Total Sugar, Acid Sugar, and Dry Matter 3

For the concentrate prepared according to Examples 10 to 16 as described in Experimental Example 1, the total sugar, acid sugar, dry matter was measured. The results are shown in the table below.

sample enzyme Enzyme addition amount (μl) Total sugar (mg / ml) Acid Sugar (㎍ / ml) Polyphenols (µg / ml) Comparative Example 3 control 0 103.12 ± 14.18 ^c 239.39 ± 37.88 ^d 346.3 ± 7.8 ^c Example 10 Rap + Visco + Cytol 100 + 100 + 100 191.45 ± 10.41 ^ab 988.89 ± 31.54 ^ab 752.1 ± 45.0 ^a Example 11 Rap + Cytol 150 + 150 178.12 ± 15.41 ^b 776.77 ± 88.78 ^bc 694.6 ± 11.3 ^b Example 12 Rap + Visco + Cytol + Eco 100 + 100 + 100 + 100 193.83 ± 14.31 ^ab 706.06 ± 124.02 ^c 758.1 ± 28.6 ^a Example 13 Visco + Cytol + Eco 100 + 100 + 100 170.98 ± 35.75 ^b 1195.96 ± 97.41 ^a 787.3 ± 40.4 ^a Example 14 Rap + Cytol + Eco 100 + 100 + 100 213.60 ± 12.32 ^a 842.42 ± 136.36 ^bc 731.3 ± 28.4 ^ab Example 15 Eco + Cytol 100 + 100 + 100 185.74 ± 19.59 ^ab 988.89 ± 201.77 ^ab 750.3 ± 23.3 ^a Example 16 Eco + Rap 150 + 150 159.79 ± 14.50 ^b 312.12 ± 138.87 ^d 735.8 ± 29.1 ^ab

As shown in Table 8, the total sugar content was 213.60 mg / ml highest when using Rapidase + Cytolase + Econase mixed (Example 14), and the content of acidic sugar was used when mixed with Viscozyme + Cytolase + Econase (Example 13) 1195.96㎍ highest in / ml. The polyphenol contents were the highest at 758.1 and 787.3 μg / ml when Rapidase + Viscozyme + Cytolase + Econase (Example 12) and Viscozyme + Cytolase + Econase were mixed (Example 13).

Experimental Example 6: Determination of Ginsenoside Content 3

The concentration of ginsenosides was measured under the same conditions as in Experimental Example 2 for the concentrate prepared according to Examples 10 to 16. The results are shown in the table below.

Ginsenoside  Concentration (㎍ / mL) Comparative Example 3 Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 Example 16 Compound K 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rh2 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rh1 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 Rg5 + RK1 0.00 0.00 190.38 197.77 207.76 265.55 259.94 285.48 Rg2 228.98 178.12 316.62 289.37 249.09 272.84 303.42 293.83 Rg3 0.00 83.17 0.00 0.00 81.32 111.94 0.00 80.90 Rg1 1087.48 939.05 1449.70 1235.31 1202.06 1362.42 1416.78 1295.23 Rf 559.98 276.55 704.78 611.30 565.82 673.77 694.29 710.31 Re 3924.71 2734.65 3698.03 3429.60 3466.66 3900.17 3911.28 4109.67 Rd 2753.69 2915.00 5123.51 3705.41 3720.15 4705.33 5254.44 4363.35 Rb2 + Rc 3285.67 2485.61 2978.58 2748.83 2771.10 3430.73 3662.56 4076.86 Rb1 6284.28 6113.00 6661.03 6487.81 6238.48 8355.04 8873.24 9193.84

As shown in Table 9, the content of Rg2 is 316.62, 303.42, 293.83 μg / mL when using a combination of Rapidase + Cytolase (Example 11), Econase + Cytolse (Example 15) and Econase + Rapidase (Example 16), respectively. In the case of Rg3, Rapidase + Cytolase + Econase (Example 14) had the highest content of 111.94 ㎍ / mL. Rb1 showed the contents of 8355.04, 8873.24 and 9193.84 μg / mL, respectively, when mixed with Rapidsae + Cytolase + Econase (Example 14), Econase + Cytolase (Example 15), and Econase + Rapidase (Example 16).

<Examples 17-21: A composition to which gamma cyclodextrin is added>

To each of the red ginseng concentrates prepared according to Examples 1 to 5 was further added Cavamax® W8 food (WACKER, Germany), which is 7 parts by weight to gamma cyclodextrin, based on 100 parts by weight of the concentrate.

Hereinafter, the composition of the present invention will be described by way of example, but it is not intended to limit the present invention but merely to explain in detail.

<Formulation Example 1: Soft Capsule>

Red ginseng concentrate prepared according to Examples 1 to 5 50mg, L-carnitine 80 ~ 140mg, soybean oil 180mg, palm oil 2mg, vegetable hardened oil 8mg, lead 4mg and lecithin 6mg, 400mg per capsule according to a conventional method Filled to prepare a soft capsule.

<Formulation Example 2: Tablet>

50 mg of red ginseng concentrate prepared according to Examples 17 to 21, 200 mg of galactooligosaccharide, 60 mg of lactose and 140 mg of maltose were mixed and granulated using a fluidized bed dryer, followed by granulating with a tableting machine by adding 6 mg of sugar ester. Tablets were prepared.

<Formulation Example 3: Granules>

 50 mg of red ginseng concentrate prepared according to Examples 1 to 5, 250 mg of anhydrous glucose, and 550 mg of starch were mixed and molded into granules using a fluidized bed granulator, and then filled into fabric.

<Formulation example 4: drink system>

50 mg of red ginseng concentrate prepared according to Examples 17 to 21, 10 g of glucose, 0.6 g of citric acid, and 25 g of liquid oligosaccharides were mixed, and 300 ml of purified water was added thereto, and 200 ml were filled in each bottle. After filling the bottle sterilized for 4 to 5 seconds at 130 ℃ to prepare a beverage.

Formulation Example 5 Caramel Formulations

Red ginseng concentrate prepared according to Examples 17 to 21 50mg, corn syrup (corn syrup) 1.8g, skim milk 0.5g, soy lecithin 0.5g, butter 0.6g, vegetable hardened milk 0.4g, sugar 1.4g, margarine 0.58g, And 20 mg of salt was mixed to form caramel.

Claims (15)

In ginseng, beta-glucanase, pectinase, hemicellulase, cellulase, arabinase and xylanase are selected from the group consisting of A method of preparing processed ginseng or processed ginseng extract, comprising increasing the content of ginsenosides by adding one or more enzymes to hydrolyze ginseng. The method according to claim 1, wherein the ginseng is red ginseng. The processed ginseng of claim 1, wherein the ginsenoside is Rg2, and the enzyme is at least one enzyme selected from the group consisting of pectinase, hemicellulase, and cellulase. Or process of preparing ginseng extract. The method of claim 3, wherein the enzyme is pectinase (pectinase). The method of claim 1, wherein the ginsenoside is Rg3, the enzyme is from the group consisting of beta-glucanase, pectinase, hemicellulase and cellulase Process for producing processed ginseng or processed ginseng extract, characterized in that at least one enzyme selected. The method of claim 5, wherein the enzyme is a combination of pectinase, hemicellulase, and cellulase. The processed ginseng of claim 1, wherein the ginsenoside is Rb1 and the enzyme is at least one enzyme selected from the group consisting of pectinase, hemicellulase, and cellulase. Or process of preparing ginseng extract. The method of claim 1, wherein the hydrolysis is performed simultaneously with the extraction of processed ginseng or processed ginseng extract. The method according to claim 1, wherein the hydrolysis is performed before extraction. Health food composition comprising a processed ginseng or ginseng extract prepared according to any one of claims 1 to 9 as an active ingredient. The health food composition of claim 10, wherein the health food composition further comprises gamma cyclodextrin. The health food composition according to claim 11, wherein the gamma cyclodextrin is contained in an amount of 5 to 20 parts by weight when the liquid ginseng extract is 100. In ginseng, beta-glucanase, pectinase, hemicellulase, cellulase, arabinase and xylanase are selected from the group consisting of Health food composition comprising a hydrolyzate obtained by adding one or more enzymes as an active ingredient. The health food composition of claim 13, wherein the health food composition further comprises gamma cyclodextrin. The health food composition of claim 14, wherein the gamma cyclodextrin is contained in an amount of 5 to 20 parts by weight when the ginseng extract is 100 liquid.