KR20090082522A - Lab on a Plate Method and Apparatus for detecting living bacteria based on selective growth and synchronized biochemical reaction - Google Patents

Abstract

A method for detecting alive bacteria is provided to reduce the time and cost by the manpower and equipment required for the preparation of culture medium. A method for detecting alive bacteria comprises the steps of preparing the detection kit of the triply sealed structure for the selective growth of the object bacteria by a selective culture component and the biochemical reaction by a selective chromophoric substrate simultaneously where the culture medium for detection is inoculated; inoculating a sample to the culture medium for detection by dilution, plating or surface contact; placing the sample-inoculated culture medium for detection inside a detector under the suitable cultivation temperature and cultivation time (wherein the cultivation condition suitable for the purpose of detection is pre-inputted in the detector so as to allow the cultivation condition suitable for detection to be set automatically by the simple manipulation on the control part of the detector); observing the color change of the colony of the detection object bacteria inside the detector by the naked eye so as to check the result; and checking the sterilization condition inside of an incubation room by checking the decoloration or discoloration with the reaction with ozone of designated concentration, thereby wasting the specimen stably.

Description

Lab on a Plate Method and Apparatus for detecting living bacteria based on selective growth and synchronized biochemical reaction

The present invention relates to a method and apparatus for detecting live bacteria which simultaneously synchronizes selective growth and biochemical reactions. The present invention relates to a detector for automatically entering a culture condition suitable for detecting a target live bacterium and automatically inducing a culture reaction with a simple operation. After culturing the target bacteria and reading the detection result, the method can be safely discarded by sterilizing the sample, and a detector for performing the process of the above method is provided to detect the live bacteria quickly and accurately. It is easy to read the result through the color reaction, so that accurate detection of live bacteria can be carried out quickly and accurately regardless of the place without any preparation by using special equipment in performing the detection method. Viable by synchronizing this possible selective growth and biochemical reactions Relates to a method and apparatus for detecting the bacteria.

As the society enters a high industrialized society, food is manufactured by industrial enterprises as processed foods or concentrated aquatic products, and after a complicated distribution process, it is increasingly consumed by end consumers through general homes, restaurants, or catering establishments.

Changes in the food environment increase the risk of microbial contamination in manufacturing and distribution processes, which often lead to food poisoning accidents caused by restaurants or group meals, raising the sense of social crisis and having a tremendous economic contraction for the industry.

This change emphasizes the necessity of systematic food safety management in production, manufacturing, and distribution for the safe food environment from microbial contamination.In addition to the existing food hygiene law, HACCP (Hazard Factor Management Standard) system has been strengthened. And coverage is also expanding day by day.

Despite the increasing trend of institutional management such as management standards, the main reason why the problems caused by microbial contamination are still not solved is that the biological hazards that cause microbial contamination in the manufacturing and distribution stages before the final consumption cannot be eliminated. Because. In particular, it is reported that the detection method that can perform the microbiological test quickly and accurately in the field and the product is very insufficient, and the lack of awareness of food safety and hygiene of employees in the manufacturing and distribution of food is also reported as an important cause.

Conventional microbial detection methods are mostly based on traditional culture methods and using specific biochemical materials. These methods must be carried out by a trained professional in a separate facility with a number of specific devices, including incubators, and are time consuming and expensive.

These limitations make it difficult for a large number of small businesses or individuals to perform microbiological testing, which is in urgent need for microbiological testing for hygiene and food safety, which in turn is a major obstacle to the completion of an institutional management system for a safe food environment. have.

Such microbial detection methods include conventional media detection methods, enzyme immunological detection methods and dry film methods.

First, the conventional medium detection method is the most common and systematically recognized detection method, and the microorganisms formed by performing a three-step procedure such as enrichment culture, selective culture, and verification by preparing a medium suitable for the target bacteria and step-by-step purposes. This is a detection method that visually observes (colony) and discriminates based on morphological characteristics or reads the result through biochemical confirmation test.

This method requires the preparation of a suitable medium for the cultivation of the target bacteria in each step, and the preparation of the medium in an appropriate container.

In order to solve this problem, recent research has been conducted to reduce costs and time by simplifying complex procedures by using a selective medium containing a selective chromogenic substrate or a selective fluorogenic substrate. However, there are still limitations in terms of time and cost in terms of expanding and distributing microbial detection to small businesses and the general public.

Due to such a complicated procedure, it takes at least three days or more to confirm the result, and there is a high cost.

In addition, it requires a separate space equipped with a variety of equipment and tools, there is a disadvantage in that a large cost of labor is required because the labor force with expertise.

In addition, enzyme immunoassay methods include recently developed microbial detection methods such as enzyme immunoassay ELISA method, DNA Chip method, antibody aggregation reaction method, polymerase chain reaction method (PCR) and the like.

These methods use the unique proteins or genes of microorganisms, including microorganisms, to detect microorganisms.They can be enriched in solid or liquid media by highly skilled experts, and then expensive and specific such as ELISA reader or PCR thermalcycler. It is a faster test technique that can detect microorganisms using equipment and reagents.

However, the enzyme immunological detection method as described above requires a very high professional manpower, and the equipment and reagents used are quite expensive, so that only a specific inspection institution can use it in a limited way. Indirect data using a bioactive material as a basis for reading, there was a problem that not only the live bacteria that cause food poisoning but also dead bacteria are not suitable for preventing food poisoning.

In addition, in the dry film method, the aqueous solution is added to a dry film medium coated with nutrients, water-soluble gels and indicators on a thin film to activate a medium, and then inoculated with a sample to incubate in a separate incubator to change color of colony by the included indicator. It is a simple detection method to read the result.

However, this method has a problem in that its use is limited to a part of the entire microbial test because the formed cells are often minute enough to be difficult to read with the naked eye and difficult to separate the bacteria for identification test.

 The main object of the present invention is to perform the appropriate human culture detection method without the expertise in microbial cultivation by allowing the appropriate culture reaction to proceed automatically by a simple selection operation through a detector input in advance suitable culture conditions for the detection target bacteria There is.

In addition, another object of the present invention is to prepare and supply a medium containing a selective culture component and a component that exhibits a selective color development substrate in advance according to the target bacteria in the detection kit, the professional personnel and equipment required in the preparation of the medium and It is to eliminate the time and cost required due to the tool and to remove the limitations of the detection method due to the need for a separate space.

In addition, another object of the present invention is to mount a detection kit containing a medium containing a selective culture component and a selective coloration substrate to a detector that satisfies the culture conditions of the target bacteria cultured by the existing enrichment culture, selective culture, definite test By shortening the screening process of three steps, such as selective culturing and biochemical reactions by selective coloration substrates in one step, the time required for the test is dramatically reduced, thus reducing the effort and cost of detection.

In addition, another object of the present invention by using a detection kit in which a medium containing a selective culture component and a selective coloration substrate is inserted into the detection kit to give a certain color to the colony of the target bacteria to be observed by visual observation This is to eliminate the need for specialized manpower and separate equipment required for reading detection results.

Furthermore, another object of the present invention is by embedding a plasma ion generating ozone sterilization device built into the detector in the detector to safely sterilize the sample cultured in the detector itself without a process through the existing separate sterilization facility company, It is to ensure that the detection method can be safely performed even in places where processing facilities and processes are difficult to equip.

In addition, another object of the present invention is to simplify the configuration of the detector to reduce the weight and size of the detector so that the detection method can be carried out even in a narrow space that is easy to carry and move.

In addition, another object of the present invention is to apply a tray for accommodating various types of detection kits inside the culture chamber of the detector, so that the detection kit in the form of a bottle or a test tube can be used as well as a plate. It is to enable the detection method.

In addition, another object of the present invention is to apply a plurality of lids to the culture chamber to be isolated from the outside to enable the reading of the results without moving the detection kit to the outside of the detector to ensure safety from microbial contamination that may originate from the sample. .

In addition, another object of the present invention is to introduce a triple structure sealed structure in the detection kit, to significantly extend the shelf life of the detection kit to ensure ease of storage and handling and to enable mass distribution.

Furthermore, another object of the present invention is to facilitate the quantitative analysis of the reading result by using a lattice protrusion structure at the bottom of the inner container of the container used in the detection kit, and to reverse the inner container and inoculate the surface contact sample. In this way, all three inoculation methods, such as dilution, swabbing, and surface contact, are possible, and thus the detection method can be applied to various types of samples.

In addition, another object of the present invention is a culture reaction suitable for the target bacteria as described above is automatically operated, a small-sized lightweight detector with its own sterilization function and a medium in which the biochemical reaction by the selective culture component and selective coloration substrate is synchronized. By inserting it into a detection kit with a special structure, it reduces the time required for the detection, effort and cost required for preparation, and eliminates the necessity of a professional manpower and separate work space and equipment for performing the detection method and reading the results. To remove it.

In addition, another object of the present invention is to enable the detection of live bacteria by small businesses or the general public, which is desperately necessary but not applicable due to the expertise and various limitations of the existing microbial testing method, and ultimately, HACCP ( The aim is to contribute to food hygiene and safety by providing a means to complement the weak parts of the management system for food safety and hygiene, including risk management.

In order to solve the above problems, a method for detecting live bacteria which synchronizes the selective growth and biochemical reaction of the present invention is a detection medium in which the selective growth of the target bacteria by the selective culture component and the biochemical reaction by the selective coloration substrate are synchronized. The detection kit in which the detection kit inoculated with a triplet has a triple sealed structure, and one of three types of sample inoculations, such as dilution, swabbing, and surface contact, is selected for detection. A sample inoculation step of inoculating the medium for incubation, and the detection medium inoculated with the sample to be placed inside the detector to satisfy the culture conditions, such as the incubation temperature and incubation time suitable for the purpose of detection, the detector Culture conditions suitable for detection purpose are pre-inputted and suitable for detection by simple selection through the detector's control panel. The culturing step to be set automatically, the detection result reading step of visually observing the color change of the detection target bacteria colony inside the detector to confirm the result, and the sterile indicator mounted inside the detector to the ozone of the specified concentration The reaction is characterized by consisting of a sterilization process and a disposal step of confirming the sterilization process again and safely discarding the sample by identifying the discoloration or discoloration reaction by checking the discoloration reaction inside the culture chamber.

The manufacturing step of the detection kit according to the present invention It consists of a kit with a special structure and a special medium inserted therein. The detection kit used here consists of a transparent inner flat container that holds the medium directly and a transparent outer container that contains the inner container again, and is packaged by double sterile packaging made of vacuum packing material to provide a safe triple sealing structure. Have

The triple sealed structure including the external container provides convenience and safety to the preservation and management of the detection kit, minimizes contact with oxygen in the air, prevents oxidation, and prevents evaporation of moisture, extending the shelf life to one year. It enables mass distribution of detection methods.

In addition, the lattice-shaped protrusion structure installed on the bottom surface of the inner container enables surface contact inoculation to vary the sample to be detected, and the lattice pattern provides convenience for quantitative analysis of the read result.

The medium used in the detection kit is E. coli, E. coli and E. coli, total number of bacteria, fungi, Listeria, Salmonella, Staphylococcus aureus, Brucella, Campylobacter, Yexinia, etc. It is made of ingredients that include color development substrates.

The detection medium that synchronizes the selective growth of the target bacterium by the selective culture component and the biochemical reaction by the selective coloration substrate allows the selective growth of only the colony of the target bacteria through the culture reaction or only the colonies of the target bacteria. It is characterized by displaying the color to enable simple, accurate and quick detection.

The inoculation step of the sample according to the present invention is capable of inoculating all three types of samples, such as dilution, swabbing, and surface contact, in which a detection kit used in a detection method is generally used. Depending on the form of the appropriate method can be selected to inoculate the sample.

Samples are inoculated on the surface of the medium contained in the inner container by various methods, and then placed in the outer container, and then the detection kit is placed in the culture chamber of the detector. In this case, the outer container of the detection kit prevents contamination of the medium contained therein, and enables stacking so that the capacity of the detector can be effectively used.

In the culturing step according to the present invention, the setting and maintenance of the culture conditions suitable for the target bacteria in the detection of live bacteria is a very important factor, and it is a factor that makes it impossible for the public to easily detect by requiring expert knowledge.

That is, in the detector, culture conditions such as E. coli, E. coli and E. coli group, total bacteria, fungi, Listeria, Salmonella, Staphylococcus aureus, Brucella, Campylobacter, Yexinia, etc. It is input in advance according to the purpose of detection, and the culture conditions suitable for detection are automatically set by the simple selection operation on the control panel and maintained for a certain time.

Therefore, it is characterized in that it is possible to perform a simple and accurate culture reaction even for a general person without the expertise required for the setting and maintenance of the culture conditions suitable for the purpose of detection.

In the reading step of the detection result according to the present invention, upon completion of the culture reaction, the colonies expressing a specific color on the surface of the medium of the detection kit are observed. It is the result of selective color reaction of colonies of interest due to biochemical reactions.

The reading of the results is done by opening only the dark brown outer openings of the double openings of the detector and visually observing colonies of color consistent with the color reaction of the detection target bacterium. In some detection methods using fluorescence substrates, small ultraviolet rays or the like mounted on a detector are irradiated with ultraviolet rays of 365 nm wavelength as necessary.

In this way, by visually observing the color change of the detection target bacteria colony and reading the results, no additional equipment is required, and even ordinary people without expert knowledge of microorganisms can read easily and accurately.

In addition, since the entire process after the inoculation of the sample is made in the culture chamber of the detector, it is characterized by minimizing the risk of microbial contamination by the sample.

In the sterilization and disposal step according to the present invention, after reading the read result, the ion plasma ozone generator mounted on the detector maintains the ozone ion concentration in the culture chamber at 1 ppm or more for 3 to 5 minutes to sterilize the sample by 99.999% or more. Prevents microbial contamination and ensures a safe biodetection environment.

The sterilization process is performed by closing the outer opening and selecting the sterilization switch of the control panel after completion of reading the result, and attaching a sterilization indicator to the container of the detection kit so that the sterilization function can be visually checked. This sterilization indicator is characterized in that it causes a discoloration or discoloration reaction in response to the ozone at a specified concentration, so as to confirm the sterilization conditions inside the culture chamber again to safely dispose of the sample.

In addition, the detection device of the living bacteria that synchronized the selective growth and biochemical reaction of the present invention is a chamber formed in the interior of the culture chamber with an upper opening, and coupled to the hinge so as to be opened and closed on one side of the culture chamber in the dark room if necessary Cover part consisting of a multi-stage hinged opaque outer cover and a transparent inner cover to meet the culture conditions, and a culture medium for synchronizing biochemical reactions by selective culture components and selective chromogenic substrates suitable for detection target bacteria. And a detection kit placed inside the culture chamber, and a vent hole is provided outside the chamber, and the outside air is introduced into the culture chamber through a heater wire from the fan module mounted therein so as to increase the culture temperature of the target bacteria inside the culture chamber. Heating module to maintain in accordance with the culture conditions time, and is mounted inside the culture chamber to release ozone And a sterilization unit for sterilizing the bacteria read after the culture, and a controller unit installed outside the chamber to control the heating module, the lamp module, and the sterilization unit to perform sterilization while satisfying the culture conditions of the target bacteria. do.

According to the present invention, the detection kit is divided into an inner container and an outer container, wherein the inner container is formed with a smear groove having an upper opening on an upper surface thereof, and is formed by forming a lattice-shaped protrusion on the bottom surface of the smear groove. While maintaining the arrangement of the medium, the outer container is provided with a storage space for accommodating the inner container, the cover formed integrally with the freely bent connection member on one side of the outer container selectively seals or opens the inner space of the outer container Characterized in that configured to be.

According to the present invention, the chamber may further include selectively mounting a tray separately provided to accommodate various medium containers in addition to the detection kit in the chamber, wherein the tray forms a settled space having an upper opening. The locking jaw is formed to protrude from the upper end of the opening to be coupled to the locking groove formed at the upper end of the culture chamber, and both sides of the inner surface of the tray are formed to face the multiple latches to accommodate the detection kit of the flat plate. And the bottom surface of the tray protrudes upward partition partition wall is characterized in that it is configured to accommodate a detection kit having a height of a polygonal shape including a circular shape on both sides of the tray divided by the partition partition wall. .

According to the invention, the ultraviolet module is characterized in that to be irradiated with a 365nm wavelength.

According to the present invention, the sterilization unit is characterized in that to generate the ozone to 100ppm / hr to sterilize the propagated bacteria.

According to the present invention, the inner container is equipped with a sterilization indicator in the form of a strip, characterized in that the sterilization indicator is configured to cause a discoloration or discoloration reaction by reacting with ozone at a concentration to ensure microbial sterilization.

According to the present invention, the controller unit and a keypad for inputting the culture medium selection key, gyro irradiation key or sterilization key by the user; A memory for storing culture temperature information and culture time information, which are culture condition information for culturing each live cell; A timer unit for counting time to drive the biodetection device according to the culture time information; A temperature sensor unit for measuring an internal temperature of the biobacteria detection device; When the culture medium selection key is selected, the culture condition information corresponding to the selected culture medium selection key is extracted, and the living cell detection device is driven according to the culture temperature information and the culture time information included in the extracted culture condition information. When the internal temperature exceeds the culture temperature while driving the bio-detection device, the culture unit is controlled to maintain the internal temperature at the culture temperature. When the ultraviolet irradiation key is input, the confirmation unit controls the projecting light to project the light. When the sterilization key is input, it characterized in that the control unit for generating the ozone for a predetermined time by controlling the sterilization unit.

According to the present invention, the controller unit is characterized in that it comprises a display unit for displaying the culture progress indication information, the remaining time information and the culture assistant information generated during the operation of the bio-detection device.

According to the invention, the controller unit is characterized in that it comprises an audio processing unit for outputting a notification sound according to the event occurs when an event occurs while driving the bio-detection device.

Therefore, the method and apparatus for detecting live bacteria which synchronized the selective growth and biochemical reactions of the present invention, the biobacteria detection method and apparatus, compared to the conventional detection method by synchronizing the selective color reaction by selective growth and biochemical reactions. Simplification reduces the time and cost of detection.

In addition, the method and apparatus for detecting live bacteria, which simultaneously synchronizes selective growth and biochemical reactions of the present invention, is provided with a culture condition suitable for a culture reaction of a target bacterium in advance, so that the culture reaction is automatically operated by a simple selection operation. There is an effect that even the general public without expert knowledge of microbial culture can perform detection of live bacteria.

In addition, the method and apparatus for detecting live bacteria which synchronized the selective growth and biochemical reactions of the present invention are characterized by the development of colonies generated through the culture reaction by synchronizing the biochemical reactions by selective culture components and selective color substrates suitable for the detection target bacteria. By visually observing the reaction, the reading of the detection result can be performed accurately and easily even by a general person without expert knowledge.

In addition, the method and apparatus for detecting live bacteria which synchronized the selective growth and biochemical reaction of the present invention by omitting the preparation process of the culture by using a detection kit containing a previously prepared detection medium in a detection kit having a special structure There is no effect of having a separate workspace, no equipment or tools, and no personnel with expertise in microbial cultivation.

Furthermore, the method and apparatus for detecting living bacteria which simultaneously synchronizes selective growth and biochemical reactions of the present invention have the effect of simplifying the construction of the detector, reducing the weight and size of the detector, making it easy to carry and move and perform the detection method even in a narrow space. There is.

In addition, the method and apparatus for detecting live bacteria which simultaneously synchronizes selective growth and biochemical reaction of the present invention safely sterilizes the cultured sample without a separate facility or treatment process through an ozone sterilizer of a plasma ion generation method built into the detector. As a result, it is possible to safely perform the detection method even in a place where it is difficult to have a separate treatment facility and process.

In addition, the method and apparatus for detecting live bacteria which synchronized the selective growth and biochemical reaction of the present invention is applied to a tray that can accommodate various types of detection kits inside the culture chamber of the detector, as well as a plate or a bottle or a test tube. It is also possible to use various types of detection kits so that a single detector can be used for various detection methods.

In addition, the method and apparatus for detecting living bacteria which simultaneously synchronizes selective growth and biochemical reactions of the present invention apply a plurality of lids to a culture chamber that is isolated from the outside, thereby enabling reading of the results without moving the detection kit to the outside of the detector. There is an effect to ensure safety from microbial contamination that may be derived from the sample.

In addition, the method and apparatus for detecting live bacteria which simultaneously synchronizes selective growth and biochemical reactions of the present invention introduces a triple structure sealed structure into the detection kit, thereby greatly extending the shelf life of the detection kit, thereby simplifying storage and handling. It has the effect of guaranteeing high quality and ensuring mass distribution.

Furthermore, the method and apparatus for detecting live bacteria which simultaneously synchronizes selective growth and biochemical reactions of the present invention uses a lattice protrusion structure at the bottom of the inner container of the container used for the detection kit to facilitate quantitative analysis of the read result. By inverting the inner container and injecting surface-contact sample, all three inoculation methods such as dilution, swabbing, and surface contact are possible. It has the effect of making it applicable.

In addition, the method and apparatus for detecting live bacteria that synchronized the selective growth and biochemical reactions of the present invention automatically operate the culture reaction suitable for the detection target bacteria as described above, and the small-sized light detector with selective sterilization and selective culture components. By inserting and using a medium synchronizing the biochemical reaction by selective coloration substrate into a detection kit with a special structure, it reduces the time required for detection and the effort and cost required for preparation, and the performance of the detection method and the reading of the results. It is effective in eliminating the need for professionals and the need for separate workspaces and equipment.

Lastly, the method and apparatus for detecting living bacteria which synchronized selective growth and biochemical reactions of the present invention are desperately needed due to the expertise and various limitations of the existing microbial testing methods, but they are not suitable for small-scale workplaces. By enabling the detection of live bacteria by the public, ultimately, they can contribute to food hygiene and safety by providing a means to supplement the weak parts of the food safety and hygiene management system including the HACCP system. It is effective.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

First, in the drawings, the same components or parts are to be noted that the same reference numerals as possible. In describing the present invention, detailed descriptions of related well-known functions or configurations are omitted in order not to obscure the subject matter of the present invention.

Figure 1 is a block diagram showing a live cell detection method of the present invention, Figure 2a is a perspective view showing a live cell detection device of the present invention, Figure 2b is a cross-sectional view showing a live cell detection device of the present invention, Figure 3a is a live cell of the present invention Figure 3b is an exploded perspective view showing a detection kit of the detection device, Figure 3b is a cross-sectional view showing a tray of the live bacteria detection device of the present invention, Figure 4 is a block diagram of a biomicrobial detection device according to an embodiment of the present invention, Figure 5 6 is a flowchart illustrating the operation of a bio-detection apparatus according to an embodiment of the present invention, and FIG. 6 is a flowchart illustrating a culture unit driving operation according to an embodiment of the present invention.

As shown in FIG. In the detection method of live bacteria of the present invention, three separate processes of additional enrichment culture, selective culture, and identification test can be omitted, and the target bacteria can be separated and cultured through one culture process. Confirmation process can be confirmed through selective growth by selective culture and selective color reaction by synchronized biochemical reaction.

In other words, the method for detecting live bacteria of the present invention comprises a manufacturing step of a detection kit, a sample inoculation step, a culture step, a reading step, a sterilization and a disposal step.

First, the manufacturing step (S10) of the detection kit is to receive a detection medium consisting of a selective growth component to selectively grow the target bacteria and a selective color substrate to induce a biochemical reaction synchronized to the detection kit having a special structure do.

Here, culture medium is composed of nutrients needed by the culture medium to culture microorganisms and animals and plants, and then mixed with substances for special purposes. In addition to water, which is indispensable for development, it supplies carbon source nitrogen source inorganic salt development factors (vitamins) as nutrients.

In other words, the living organisms require water, indispensable for survival and development, and as a nutrient, a microelement, a microelement. Except those obtained in the gas phase, all should be supplied to the medium as inorganic or organic compounds, and the required compounds are various depending on the nutritional form such as independent nutrition and heterotrophs. In general, nutrients are divided into carbon source nitrogen source, inorganic salts, growth factors (vitamins, etc.), and natural mediums are known to have a relatively complex composition extracted from living organisms. Synthetic medium is called a case where the composition of a nitrogen or nitrogen source with a definite structure is added.

In addition, a liquid medium is suitable for large-scale cultivation of live bacteria, and a solid medium to which agar gelatin or the like is added is used for preservation or separation of liquor. A medium for extracting the target bacteria from a material containing many kinds of bacteria is called a selective medium.

In the present invention, by using the selective culture component of the selection medium with a selective coloration substrate that induces a biological response synchronized with this, the viable bacteria can be detected by visually observing the selective growth of a specific target bacterium and the specific color reaction of a colony of the target bacteria. do.

Next, the sample inoculation step (S20) is a step of inoculating samples taken from various materials on the surface of the detection medium accommodated in the inner container of the detection kit. At this time, the method of inoculating the sample may be applied to all commonly used dilution, swabbing, surface contact (Surface Contact) and the like.

The detection kit having the conditions for culturing the target bacteria is transferred to the incubation chamber of the detector, which is isolated from the outside, and placed therein, thereby providing a culture reaction condition of constant temperature for a predetermined time to be suitable for the detection of the target bacteria. It goes through (S30).

Here, suitable culture conditions (temperature, temperature holding time) for each target bacteria will be described in detail in the following Examples.

After the culture reaction is completed, the result of the detection is confirmed through the reading step (S40). At this time, the colony of the detection target bacterium is displayed in a specific color by the selective culture and the biochemical reaction synchronized with it, so that the non-expert can easily and accurately read the result.

For example, when the total coliform test is performed according to the conventional E. coli test method, the reading of the result confirms the typical form of the E. coli group by observing colonies formed on the medium to determine the presence of E. coli group. It may be determined tentatively or determined by a separate indicator treatment or biochemical test.

However. In the viable cell readout step (S40) of the present invention, due to the selective cultivation of the detection medium accommodated in the detection kit and the specific color reaction through the biochemical reaction synchronized with the detection kit, only the colony of a specific color is formed. The detection result reading can be performed easily and accurately, and can be performed by the general public.

As described above, the detection medium provided by the detection kit of the present invention forms a colony in which only microorganisms corresponding to the test purpose are displayed in a specific color even without observing typical forms of viable colonies or processing a separate indicator after incubation. To do it.

In addition, the detection medium in the reading step (S40) may induce a fluorescence reaction in addition to the color reaction depending on the detection purpose, so that only the target microorganism selectively causes a color change to a constant fluorescence color. It is made by irradiating ultraviolet rays of 365 nm wavelength through a small ultraviolet lamp installed in the.

Finally, the live bacteria read through the sterilization step (S50) to sterilize the bacteria generated through the ozone by the plasma ion discharge, so that ozone is generated for about 10 seconds or more to reach an ozone concentration of 1.0 ppm or more inside the culture chamber. Within 2 to 3 minutes, it has a bactericidal effect of more than 99.99% against most microorganisms including mold.

In addition, the culture of the live bacteria of the present invention. Read and sterilization method may be performed live gas reading step (S40) to detect only the target bacteria to perform lactose (lactose) fermentation after the culture step (S30).

In other words, if microorganisms belonging to Coliform (Coliform) performing lactose fermentation are cultured, the presence of bubbles generated by gas generated is visually checked. If bubbles are present, positive coliforms are detected. E. coli group can be read as negative to go through the reading process of the microorganism to perform lactose fermentation and then end with the sterilization step (S50).

As above. Cultivation of live bacteria. The configuration of the detector 100 capable of automatically performing the reading and sterilization method is as follows.

As shown in FIGS. 2A to 3B, a multi-stage opening is provided in which the culture chamber 111 having an upper opening is hinged to be opened and closed at one side of the culture chamber 111 so as to have a dark room culture condition in the culture chamber 111. A cover portion 120 consisting of a hinged opaque outer cover 121 and a transparent inner cover 122, and a medium for survival and development of the target bacteria, wherein the medium includes a selective medium, a selective chromogenic medium, a selective fluorescent medium. the detection kit 130 placed inside the culture chamber 111 by adding an indicator for reading composed of fluorogenic media, independently or in combination, and an air outlet is provided outside the chamber 100, and external air is supplied from the fan module 141 mounted therein. The incubation temperature of the target bacteria in the incubation chamber 111 is allowed to enter the incubation chamber 111 through the exothermic module 142. When the target bacteria are colonized by the culturing unit 140 and the culture chamber 111 and the heating module 142 and the selective fluorogenic medium culture conditions, the target bacteria are displayed in fluorescent color by ultraviolet rays. A readout unit 150, a sterilization unit 160 mounted inside the culture chamber 111 to sterilize bacteria read after incubation by generating ozone, and a heating module 142, a lamp module, and a sterilization unit 160 installed outside the chamber 100. It consists of a controller unit 170 to control the sterilization while satisfying the culture conditions of the target bacteria.

The chamber 100 is provided with a culture chamber 111 therein, and includes a cover that is hinged to isolate the culture chamber 111 from the outside.

The detection kit 130 is placed in the culture chamber 111 is placed in the culture chamber 111 is divided into an inner container 131 and an outer container 132, the inner container 131 is formed with a smear groove 131a having an upper opening on the upper surface, the bottom surface of the smear groove 131a Forming grooves 131a in the form of a lattice in the form to maintain the placement state of the smeared medium, the outer container 132 is provided with a receiving space 132a for accommodating the inner container 131, the connection member 133 freely bent on one side of the outer container 132 The cover formed integrally with the configuration is configured to selectively seal or open the inner space of the outer container 132.

In addition, the inner container 131 is housed in the outer container 132 and made of a transparent material to visually read the discoloration and fluorescence discoloration of live bacteria of the inner container 131 with the naked eye from the outside, and smear groove on the bottom surface of the smear groove 131a of the inner container 131 By forming the 131a in a lattice form, it is possible to easily grasp the function of preventing and fixing the release of the medium and the colony count when performing detection of live bacteria by surface contact inoculation.

In addition, the culture unit 140 allows the culture chamber 111 and the external space to be ventilated through the tuyeres provided on the outside of the chamber 100, and generates a heating module made of constituent means such as hot wires by introducing external air by the fan module 141 mounted inside the tuyeres. When the outside air is introduced into the culture chamber 111 through the 142, the air having a temperature adjusted to the culture conditions (temperature) of the target bacteria is supplied into the culture chamber 111.

In addition, the readout unit 150 is used to read live bacteria through a selective fluorescence medium, and consists of an ultraviolet lamp inside the culture chamber 111 so that ultraviolet rays are irradiated at an intensity of 365 nm so that a specific fluorescent color can be exerted according to the target bacteria. As a result, the fluorescent color change inside the inner container 131 can be easily read from the outside with the naked eye.

In addition, the sterilization unit 160 is mounted inside the culture chamber 111 to generate an ozone inside the culture chamber 111 through a plasma ion device that generates ozone to sterilize the bacteria after reading, but the ozone at an intensity of 100ppm / hr or more It is generated for about 10 seconds or more so that the inside of the culture chamber 111 reaches a concentration of 1.0 ppm of ozone to have a sterilizing effect of 99.99% or more for most microorganisms including mold within 2 to 3 minutes.

In particular, the inner container is equipped with a sterilization indicator 161 in the form of a strip to react with ozone at a concentration that guarantees microbial sterilization so that the sterilization indicator 161 may cause a discoloration or discoloration reaction. Make it understandable.

In addition, the culture chamber 111 of the chamber 100 may further include selectively mounting a tray 180 separately configured to accommodate various medium containers in addition to the detection kit 130, wherein the tray 180 has an upper settled space 181. And forming a locking projection 182 to protrude into the open upper end to be coupled to the locking groove 112 formed in the upper end of the culture chamber 111, and to form a multi-level locking clasp 183 on both sides of the inner surface of the tray 180. The detection kit 130 of the flat plate can be stored, and the partition bulkhead 184 protrudes upward from the bottom surface of the tray 180 to have a polygonal shape including a circular shape in both spaces of the tray 180 divided by the partition bulkhead 184. The detection kit 130 can be stored.

The tray 180 utilizes a variety of shapes and capacities of petri dishes, conical tubes and Bayers, and specially designed culture containers by compactly stacking specific containers or all container shapes according to their shapes and capacities to efficiently utilize space. To increase its utilization.

Further, the keypad 171 and the culture medium selection key, ultraviolet irradiation key or sterilization key is input by the user; A memory 172 for storing culture temperature information and culture time information, which are culture condition information for culturing each live cell; A timer unit 173 for counting time to drive the biodetection device according to the culture time information; A temperature sensor unit for measuring an internal temperature of the biobacteria detection device; When the culture medium selection key is selected, the culture condition information corresponding to the selected culture medium selection key is extracted, and the biocide detection device is driven according to the culture temperature information and the culture time information included in the extracted culture condition information. If the internal temperature exceeds the culture temperature during driving of the bio-detection device, the culture unit 140 is controlled to maintain the internal temperature at the culture temperature, and when the ultraviolet irradiation key is input, the light is controlled to project the light. When the sterilization key is input, the control unit 175 controls the sterilization unit 160 to generate ozone for a predetermined time.

The controller includes a display unit 177 for displaying the culture progress indication information, the remaining time information, and the culture preconditioning information generated during the driving of the biobacterial detection device. When an event occurs during the driving of the biodetection device, the controller according to the generated event And an audio processor 174 for outputting a notification sound.

Figure 4 is a block diagram of a biomicrobial detection apparatus according to an embodiment of the present invention. Referring to Figure 4 will be described the structure of the bio-detection device. The biodetection apparatus includes a controller unit 170, a culture unit 140, and a sterilization unit 160. The controller unit 170 includes a keypad 201, a memory 403, a timer unit 405, an audio processor 407, an image processor 409, a display unit 411, a temperature sensor unit 413, and a controller 423. The culture unit 140 includes a heating module 415 and a fan module 417. In addition, the reading unit 150 includes a lamp module 419, the sterilization unit 160 includes an ozone generating unit 421.

The keypad 201 includes keys for inputting numeric and text information and function keys for setting various functions. In addition, according to an embodiment of the present invention, the keypad 401 includes a culture selection key, an ultraviolet irradiation key, a sterilization key, a setting key, and an ending key. In addition, a user may input culture condition information for culturing each live cell using the keypad 401 and store and set it in the memory 403.

The memory 403 may be composed of a program memory and a data memory. The program memory stores a program for controlling a general operation of the biodetection apparatus. In addition, data generated during the execution of the programs are temporarily stored in the data memory. According to an embodiment of the present invention, the data memory stores the culture temperature information and the culture time information, which are the culture condition information for culturing each live bacterium, according to the type of the bacterium. For example, the E. coli and E. coli group is stored with the culture temperature information is set to 35 ~ 37 ℃, the culture time information is set to 24 hours and stored, Clostridium perfringens bacteria is stored with the culture temperature information is set to 44 ℃ Incubation time information is stored and set to 24 hours.

The timer unit 405 counts the time when the biomicrobial detection apparatus is driven to culture the bacteria according to an embodiment of the present invention. This is to drive the bio-detection device for a predetermined incubation time.

The audio processor 407 may configure a codec, and performs a function of converting and reproducing an analog signal through the audio codec. According to an embodiment of the present invention, the audio processor 407 outputs a notification sound through a speaker when an event (biodetection device driving, termination, etc.) occurs.

The image processor 409 transmits the generated image signal in accordance with the standard of the display unit 411, and also compresses and decompresses the image data. In addition, the start address value of the image data output to the display unit 411 is transmitted or changed by setting the start address value. In addition, according to an embodiment of the present invention, the culture progress indication information, the remaining time information and the culture aid information is processed to be displayed on the display unit 411.

The display unit 411 displays image data output from the image processor 409. In this case, the display unit 411 may use an LCD. In this case, the display unit 411 includes an LCD controller, a memory for storing the culture progress indication information, the remaining time information, and the culture assistant information, and an LCD display element. can do.

The temperature sensor unit 413 performs a function of measuring the temperature in the biomicrobial detection apparatus according to an embodiment of the present invention. The temperature sensor 413 may be an NTC thermistor sensor using a change in electrical resistance according to a temperature, or a PTC thermistor sensor using a change in electrical resistance in a Curie temperature of a semiconducting barium titanate.

The heating module 415 may be an electric heater or a heater of an electron tube directly using a joule heat generated by passing a current through a resistance wire, etc., according to an embodiment of the present invention, the heating module 415 is a culture temperature at which the temperature in the bio-detection device is set. Perform a function to reach

According to an embodiment of the present invention, the fan module 417 introduces external air into the biomicrobe detection device or discharges the air inside the biocide detection device to the outside according to the rotation direction of the fan. It serves to maintain the temperature of the set incubation temperature.

The lamp module 419 is irradiated to show the fluorescence phenomenon of the cultured bacteria according to an embodiment of the present invention, and irradiates ultraviolet rays of 365nm wavelength band to sterilize contamination by the introduced external air.

The ozone generator 421 functions to remove secondary contamination by sterilizing the cultured live bacteria by generating more than 100ppm / hr of ozone for a predetermined time (10 seconds) when the biomicrobe detection device is driven in accordance with an embodiment of the present invention. Do this.

The controller 423 performs a function of controlling the overall operation of the biodetection apparatus. And the codec. In addition, the control unit 423 controls to extract the culture condition information corresponding to the selected culture medium selection key, when the culture medium selection key is input by a user request according to an embodiment of the present invention, the extracted culture condition information According to the culture temperature information and the culture time information contained in the control unit to drive the culture unit provided in the bio-detection device, when the ultraviolet irradiation key is input, driving the confirmation unit, when the sterilization key is input, the sterilization unit is controlled To generate ozone for a predetermined time. And the control unit 423 is in accordance with one embodiment of the present invention, if the internal temperature exceeds the culture temperature during driving the bio-detection device (culture unit) according to the culture conditions, the outside air flows into the bio-bacteria detection device The internal temperature is controlled to be maintained at the culture temperature.

Figure 4 is a block diagram of a biomicrobial detection apparatus according to an embodiment of the present invention. Referring to Figure 4 will be described the structure of the bio-detection device. The biodetection apparatus includes a controller unit 170, a culture unit 140, a read unit 150, and a sterilization unit 160. The controller unit 170 includes a keypad 171, a memory 172, a timer unit 173, an audio processor 174, an image processor 176, a display unit 177, a temperature sensor unit 178, and a controller 175. The culture unit 140 includes a heating module 412 and a fan module 177.

The keypad 171 includes keys for inputting numeric and text information and function keys for setting various functions. In addition, according to an embodiment of the present invention, the keypad 171 includes a culture selection key, an ultraviolet irradiation key, a sterilization key, a setting key, and an ending key. In addition, a user may input culture condition information for culturing each live cell using the keypad 171 and store and set it in the memory 172.

The memory 172 may be composed of a program memory and a data memory. The program memory stores a program for controlling a general operation of the biodetection apparatus. In addition, data generated during the execution of the programs are temporarily stored in the data memory. According to an embodiment of the present invention, the data memory stores the culture temperature information and the culture time information, which are the culture condition information for culturing each live bacterium, according to the type of the bacterium. For example, the E. coli and E. coli group is stored with the culture temperature information is set to 35 ~ 37 ℃, the culture time information is set to 24 hours and stored, Clostridium perfringens bacteria is stored with the culture temperature information is set to 44 ℃ Incubation time information is stored and set to 24 hours.

The timer unit 173 counts time when the bio-detection device is driven to culture the bacteria according to one embodiment of the present invention. This is to drive the bio-detection device for a predetermined incubation time.

The audio processor 174 may configure a codec and performs a function of converting and reproducing an analog signal through the audio codec. According to an embodiment of the present invention, the audio processor 174 outputs a notification sound through a speaker when an event (biodetection device driving, termination, etc.) occurs.

The image processing unit 176 transmits the generated image signal in accordance with the standard of the display unit 177, and also compresses and decompresses the image data. Also, the start address value of the image data output to the display unit 177 is transmitted or the start address value is changed and set. In addition, according to an embodiment of the present invention, the culture progress indication information, the remaining time information and the culture condition information is processed to be displayed on the display unit 177.

The display unit 177 displays image data output from the image processor 176. In this case, the display unit 177 may use an LCD. In this case, the display unit 177 may include an LCD controller, a display for displaying culture progress information, remaining time information, and a culture display information, and an LCD display device. can do.

The temperature sensor unit 178 performs a function of measuring the temperature in the biomicrobial detection apparatus according to an embodiment of the present invention. The temperature sensor unit 178 may be an NTC thermistor sensor using a change in electrical resistance according to a temperature, or a PTC thermistor sensor using a change in electrical resistance in a Curie temperature of a semiconducting barium titanate.

The heating module 142 may be an electric heater or a heater of an electron tube directly using a joule heat generated by passing a current through a resistance wire, etc., according to an embodiment of the present invention, the heating module 142 is a culture temperature at which the temperature is set in the bio-detection device. It performs the function of reaching.

The fan module 141 according to an embodiment of the present invention according to the rotation direction of the fan (fan) inflow of the outside air into the bio-detection device or discharge the air inside the bio-detection device to the outside to the inside of the bio-detection device It serves to maintain the temperature of the set incubation temperature.

The reading unit 150 may be a lamp module, and irradiated to show a fluorescence phenomenon of the cultured bacteria according to an embodiment of the present invention, and ultraviolet rays of 365 nm wavelength in order to sterilize contamination by introduced external air. Investigate

The sterilization unit 160 may be an ozone generating module, and in accordance with an embodiment of the present invention, at the end of driving the microbial detection apparatus, ozone is generated at 100 ppm / hr for a predetermined time (10 seconds) to sterilize the cultured live bacteria 2 Function to remove secondary pollution.

The controller 175 performs a function of controlling the overall operation of the biodetection apparatus. And the codec. In addition, the control unit 175 controls to extract the culture condition information corresponding to the selected culture medium selection key, when the corresponding culture medium selection key is input by the user's request according to an embodiment of the present invention, the extracted culture condition information In accordance with the culture temperature information and the culture time information contained in the control unit to drive the culture unit provided in the bio-detection device, and when the self-irradiation key is input, the reading unit 150 is driven, when the sterilization key is input, The sterilization unit 160 is controlled to generate ozone for a predetermined time. In addition, the controller 175 is driven in accordance with the culture conditions in accordance with the culture conditions when the internal temperature exceeds the culture temperature during the driving of the bio-detection device (culture unit), the outside air is introduced into the bio-bacteria detection device The internal temperature is controlled to be maintained at the culture temperature.

FIG. 5 is a flowchart illustrating the operation of a biomicrobial detection apparatus according to an embodiment of the present invention, and FIG. 6 is a flowchart illustrating a culture unit driving operation according to an embodiment of the present invention. Referring to FIGS. 5 and 6, the culture condition information corresponding to the type of culture medium selected by the biocide detection device is extracted, and the driving operation of the biobacteria detection device according to the extracted culture condition information is described. In step S, when the user inputs the culture medium selection key by using the keypad 171, the controller 175 detects this in step 503, proceeds to step 505, and analyzes the input culture medium selection key information in step 507. In step 507, the controller 175 controls the memory 172 to extract culture condition information corresponding to the input culture selection key information. For example, when the user enters the E. coli and E. coli group selection key, the culture temperature information is 35 ~ 37 ℃ and the culture time information is extracted for 24 hours, the user selects Clostridium perfringens bacteria If a key is input, the culture condition information of 44 ° C. and the culture time information is 24 hours is extracted.

In operation 509, the controller 175 controls the culture unit 140 including the heating module 142 and the fan module 141 to drive the culture unit 140 according to the extracted culture condition information. The operation of the culture unit 140 will be described in more detail with reference to FIG. 6. In step 601, the controller 175 controls the temperature sensor unit 178 to check the current internal temperature of the biocide detection device. Compare the current internal temperature and the extracted culture temperature information. As a result of the comparison, when the current internal temperature is equal to or lower than the culture temperature, the heating module 142 is driven and the fan module 141 is stopped. On the other hand, as a result of the comparison, if the current internal temperature is greater than the incubation temperature, the heat generating module 142 is stopped and the fan module 141 is driven.

When the culture unit 140 is driven, the controller 175 controls the timer unit 173 in step 511 to count time and proceeds to step 513. This is to drive the bio-detection device during the extracted incubation time. In step 513, the controller 175 compares the counted time with the incubation time. As a result of the comparison, if the counting time does not arrive at the incubation time, the control unit 175 proceeds to step 509 and continues to drive the culture unit 140. On the other hand, if the counting time does not arrive at the incubation time as a result of the comparison, the process proceeds to step 517 and stops driving the culture unit 140.

Then, when the user inputs the ultraviolet irradiation key using the keypad 171, the control unit 175 detects this in step 519 and proceeds to step 521. In step 521, the control unit 175 irradiates light by driving the reading unit (for example, a lamp module) 150.

Next, when the user inputs the sterilization key using the keypad 171, the controller 175 detects this in step 523 and proceeds to step 525. In step 525, the control unit 175 generates the ozone for a predetermined time by driving the sterilization unit (for example, the ozone generating unit) 160.

As such, cultivation of live bacteria of the present invention. Examples of the reading and sterilization method and apparatus and the medium composition for growing the target bacteria to be cultured by the control system and the results of the reading are as follows.

Example  One

E. coli and coliform (E. coli & coliform) is cultured as a selective coloration used to read after the cultivation and the selective coloration medium by the biochemical reactions synchronized to it is shown in Table 1.

TABLE 1

 Ingredients  Grams / Liter  Peptone special  5.00  Sodium chloride  5.00  Sorbitol  1.00  Dipotassium hydrogen phosphate  2.70  Potassium dihydrogen phosphate  2.70  Sodium laurly sulphate  0.10  Chromogenic substrate  0.08  Fluorogenic substrate  0.05  IPtage (IPTG)  0.10  Agar  15.00

After inoculating the sample into the detection kit containing the medium consisting of the above composition and placed in the culture chamber, close the double cover and select the selection menu E. coli and E. coli group detection on the keypad of the control unit.

Afterwards, the culture reaction for 24 hours at 35 ~ 37 ℃ is automatically progressed by the detector. When the completion of culture indicator is turned on in the progress indicator located on the display part of the keypad, only the outer cover of the double opening is opened. The detection results are read by observing the surface of the detection medium contained in the detection kit.

After the reading is completed, selecting the sterilization button on the detector keypad automatically releases ozone to sterilize the detection kit including the inside of the culture chamber and the sample.

After that, when the sterilization process is completed, the safety indicator of the display unit turns on. After checking the detection kit, the detection kit is taken out of the detector to check the discoloration of the sterilization indicator attached to the detection kit and then discharged together with general waste.

The detection of the detection result can be confirmed by checking the fluorescence color by operating an ultraviolet (UV lamp) of the reading unit installed in the upper part of the culture chamber after the culture is completed, the fluorogenic substrate of the composition of the E. coli It is decomposed by the specific enzyme beta-glucuronidase (β-glucuronidase) and shows fluorescence when irradiated with UV rays of 365 nm wavelength, and the chromogenic substrate is decomposed by the specific enzyme of Escherichia coli group. Will be shown.

Therefore, only the colonies of E. coli (colony) is observed in the blue-green fluorescence when the UV irradiation of the 365nm wavelength after the end of the culture, and the blue-green colonies observed when the UV lamp (UV lamp) is turned off to observe the colonies of E. coli.

The detailed reading of detection results follows the table <1-1>.

TABLE 1-1

   Strain (ATCC)  Colony  Fluorescence  Indole reaction  Enterobacter aerogenes (13048)  Turquoise  -  -  E. coli (25922)  Turquoise  +  +

Example  2

The selective chromogenic medium by biochemical reactions synchronized with the selective culture used for culturing Pseudomonas species and then reading them is composed of <Table 2>.

TABLE 2

 Ingredients  Grams / Liter  Gelatin (Pancreatic digest of gelatin)  18.00  Magnesium chloride  1.40  Potassium sulphate  10.00  Cetrimide  0.30  Fluorogenic mixture  2.05  Agar  5.00

Operate the detector and detection kit in the same manner as in <Example 1>, and select Pseudomonas bacteria detection from the selection menu of the detector keypad. Afterwards, the incubation for 24 hours at 35 ° C. is automatically performed by the detector.

Reading of the detection result confirms the detection result by operating the ultraviolet lamp (UV lamp) installed in the upper part of the culture chamber and confirming the fluorescence coloration. This is due to the use of a fluorogenic mixture of the composition (Pseudomons aeruginosa) Pseudomons aeruginosa (Pseudomons aeruginosa) decomposes the fluorogenic mixture (fluorogenic mixture) to cause fluorescence by ultraviolet light of 365nm wavelength. Therefore, a colony showing fluorescence when the UV lamp is operated and observed after incubation is read by Pseudomon saeruginosa.

The detailed reading of detection results follows the table <2-1>.

<Table 2-1>

 Strain (ATCC)  growth  Fluorescence  E. coli (25922)  Inhibited   -  P. aeruginosa (27853)  Good   +  Pseudomonas maltophila (13637)  Inhibited   -  Staphylococcus maureus (S. aureus) (25923)  Inhibited   -

Example 3

E. coli cultivation of E. coli and selective cultivation medium by biochemical reactions simultaneously with the selective culture used to read it is formulated in <Table 3>.

TABLE 3

 Ingredients  Grams / Liter  Casein (Casein enzymic hydrolysate)  20.00  Bile salt mixture  1.50  L-tryptophan  1.00  Lactose  5.00  Sodium chloride  5.00  Dipotassium hydrogen phosphate  4.00  Potassium dihydrogen phosphate  1.50  Fluorogenic substrate  0.07  Chromogenic substrate  0.10  Agar  15.00

After the detector and the detection kit are operated in the same manner as in <Example 1>, E. coli / E. Coli group detection is selected from the selection menu of the detector keypad. After 24 hours at 35 ~ 37 ℃ culture reaction is automatically carried out by the detector.

Reading of the detection result confirms the detection result by activating an ultraviolet lamp (UV lamp) installed in the upper part of the culture chamber after the incubation is completed and confirming the fluorescence. Similar to Example 2, but the chromogenic mixture used at this time is a substrate which is specifically decomposed by E. coli and shows a bluish-colored phenomenon. Therefore, this method is a method of detecting E. coli by fluorescence coloration when irradiated with ultraviolet rays of 365 nm wavelength and by bluish green colony when observed without irradiation with ultraviolet rays.

The detailed reading of detection result follows the table <3-1>.

<Table 3-1>

 Strain (ATCC)  growth  Colony color  Fluorescence  Indole reaction  E. coli (25922)  Good  Bluish-green   +   +  K. pneumoniae (13883)  Good  Colorless (cloroless)   -   -  P. aeruginosa (27853)  Good  Colorless   -   -  S.faecalis (29212)  Inhibited   -   -   -

Example  4

E. coli and Coliform (E. coli & Coliform) is cultured as a selective coloration medium by a biochemical reaction synchronized with the selective culture used to read after the culture is shown in Table 4.

TABLE 4

 Ingredients  Grams / Liter  Peptone  5.00  Yeast extract  3.00  Lactose  2.50  Disodium hydrogen phosphate  3.50  Monopotassium dihydrogen phosphate  1.50  Sodium chloride  5.00  Neutral red  0.03  Chromogenic mixture  20.3  Agar  15.00

The operation of the detector and the detection kit is performed in the same manner as in Example 1, and the E. coli and E. coli group detection is selected from the selection menu of the detector keypad. Afterwards, the incubation for 24 hours at 35 ° C. is automatically performed by the detector.

In the composition, the chromogenic mixture is hydrolyzed by glucoronidase, an enzyme specifically produced by Escherichia coli, to produce a purple color reaction only for colonies formed by E. coli. It is composed of chromogen two, which is hydrolyzed by chromogen one and galactosidase, an enzyme produced by E. coli, which causes red color reaction only for colonies formed by E. coli. .

Therefore, purple colonies are read with E. coli and red colonies with E. coli.

The detailed reading of detection result follows the table <4-1>.

<Table 4-1>

 Strain (ATCC)  growth  Colony color  E. coli (25922)  Flourishing  Blue / Purple  K. pneumoniae (13883)  Flourishing  Rose pink  Pseudomonas aeruginosa (P.aeruginosa) (13883)  Good-luxurinat  Brown (Straw)

Example  5

Escherichia coli 0157: H7 (E.Coli 0157: H7) is cultured into selective coloration media by biochemical reactions synchronized with the selective culture used to read them.

TABLE 5

 Ingredients  Grams / Liter  Casein (Casein enzymic hydrolysate)  17.00  Proteose peptone  3.00  Sorbitol  10.00  Bile salt mixture  1.50  Sodium chloride  5.00  Crystal violet  0.001  Neutral red  0.03  B.C. indicator  0.10  Agar  13.50

Operate the detector and the detection kit in the same manner as in <Example 1>, and select detect E. coli O157: H7 from the selection menu of the detector keypad. Thereafter, the culture reaction for 18 to 24 hours at 35 ~ 37 ℃ is automatically proceeded by the detector.

E. coli O157: H7 (Escherichia coli O157: H7) uses lactose for metabolism, but sorbitol cannot be used for metabolism, while other E. coli species use sorbitol for metabolism. Produces glucuronidase (βD-glucuronidase). The B.C indicator in the composition material reacts with the beta glugluronidase (β Dglucuronidase) to produce a blue-green color reaction. Therefore, among the colonies that occurred, the blue-green colonies were colonies of E. coli and the colonies of E. coli O157: H7 became colorless. In other words, when colorlessness was observed among the colonies generated, E. coli O157: H7 was detected.

The detailed reading of detection result follows the table <5-1>.

Table 5-1

 Strain (ATCC)  growth  Colony color  Escherichia coli O157 (E. coli O157: H7) (NCTC 12900)  Good luxuriant  Colorless  E. coli (25922)  Good  Blue-green  Pseudomonas aeruginosa (P.aeruginosa) (27853)  Poor-good  Colorless  Listeria pneumoniae (L.neumoniae) (13883)  Good  Pink-red

Example  6

The selective chromogenic medium by biochemical reactions synchronized with the selective culture used to read Salmonella species after culturing is formulated in <Table 6>.

TABLE 6

 Ingredients  Grams / Liter  Peptic digest of animal tissue  6.00  Yeast extract  2.50  Bile sat mixture  1.00  Chromogenic mixture  5.40  Agar  13.00

After operating the detector and the detection kit in the same manner as in Example 1, Salmonella detection is selected from the selection menu of the detector cap. After 24 hours at 35 ~ 37 ℃ culture reaction is automatically carried out by the detector.

Salmonella bacteria use propylene glycol to metabolize specifically to produce acids. The acid reacts with chromogenic mixtures to form pink colonies, while E. coli forms cyan colonies due to beta glucuronidase, which is clearly distinguishable.

The detailed reading of detection result follows the table <6-1>.

Table 6-1

 Strain (ATCC)  growth   Colony color  E. coli (25922)  Flourishing   Blue-green  Salmonella S. serotype Enteritidis (13076)  Flourish (Luxuriant0  Light purple with halo  Salmonella Typhi (6539)  Good-luxuriant  Light purple with halo  S. serotype Typhimurium (14028)  Flourishing  Light purple with halo  P. vulgaris (1315)  Good  Colorless  Staphylococcus anureus (25923)  Inhibited   -  B. subtilis (6633)  Inhibited   -

Example  7

The selective chromogenic medium by biochemical reactions synchronized with the selective culture used for culturing fungi and fungi (Yeast & Mould) and then reading them is shown in <Table 7>.

TABLE 7

 Ingredients  Grams / Liter  Yeast extract  4.00  Dextrose  20.00  Chromogenic mixture  1.10  Agar  12.00

After the detector and the detection kit are operated in the same manner as in Example 1, fungal detection is selected from the selection menu of the detector cap. Then, the culture reaction for 48 hours at 25 ~ 30 ℃ is automatically carried out by a detector, and then goes through the same process as in <Example 1>.

Fungi specifically use oxytetracycline for metabolism, and oxytetracycline reacts with chromogenic mixtures, and the colonies of Aspergillus niger are set against white spores. Black colonies form and colonies of Candida albicans form green cultures.

The detailed reading of detection result follows the table <7-1>.

Table 7-1

 Strain (ATCC)  Colony growth  Colony color  Aspergillus Niger (16404)  Flourishing  Light blue with black spores  Candida Aricans (10231)  Flourishing  Green  E. coli (25922)  Inhibited   -  S. cerevisiae (19615)  Flourishing   Colorless

Example  8

The selective color development medium by biochemical reactions synchronized with selective culture used for culturing Enterobactr sakazakii and then reading them is shown in Table 8.

TABLE 8

 Ingredients  Grams / Liter  Casein enzymic hydrolysate  15.00  Peptic digest of soyabean meal  5.00  Sodium chloride  5.00  Sodium deoxycholate  0.50  Sodium thiosulphate  1.00  Chromogenic mixture  10.17  Agar  15.00

After the detector and the detection kit are operated in the same manner as in Example 1, detection of the bacterium bacterium is selected from the selection menu of the detector cap. Since the culture reaction for 24 hours at 35 ~ 37 ℃ is automatically carried out by a detector, and then goes through the same process as in <Example 1>.

The chromogenic mixture of the above components is decomposed by acting as a substrate of glucosidase specific for Enterobacter (Enteorbacter) bacteria to stain the colonies of Enterobacter sarcoma bacterium blue.

The detailed reading of detection result follows the table <8-1>.

Table 8-1

 Strain (ATCC)  Colony growth  Colony color  E. coli (25922)  Flourishing  Yellow  Enterobacter erogenes (13048)  Flourishing  Bluish green  Enterobacter pecalis (29212)  Inhibited  -  Enterobacter (E.sakazaki) (12868)  Flourishing  Blue  Staphylococcus aureus (25923)  Inhibited   -  K. pneumoniae (13883)  Flourishing  Yellow (mucoid)

Example  9

Selective color medium by biochemical reactions synchronized with the selective culture used to read Listeria spp. (Listerria species) and then read it is prepared in Table 9.

TABLE 9

 Ingredients  Grams / Liter  Peptone special  23.00  Sodium chloride  5.00  Yeast extract  1.00  Meat extract  5.00  Lithium chloride  5.00  Rhamnose  10.00  Phenolic red  0.12  Chromogenic mixture  5.13  Agar  15.00

Operate the detector and the detection kit in the same manner as in <Example 1>, and then select Listeria bacteria detection from the selection menu of the detector cap. Then, the culture reaction for 24 hours at 35 ~ 37 ℃ is automatically carried out by a detector, and then goes through the same process as in <Example 1>.

Listeria has PIPLC (phosphatidylinositol specific phospholipase C) activity and decomposes a chromogenic mixture to show blue color. In this case, rhamnose among the components forms a red background by fermentation by Listeria ivanovii among Listeria bacteria, whereas Listeria monocytogenes and Listeria innocua ) Does not ferment to form a yellow background. That is, Listeria bacteria form a blue colony, of which Listeria monocytogene or Listeria innocua is formed on a yellow background, and Blue colony formed on a red background is Listeria Ivano. The ratio (Listeria ivanovii) is shown.

After operating the detector and the detection kit in the same manner as in <Example 1>, the Staphylococcus detection is selected in the selection menu of the detector cap. Since the culture conditions of 35 ~ 37 ℃ is automatically maintained by the incubator for 24 hours and then goes through the same process as in <Example 1>.

The chromogenic mixture of the composition is staphylococcus is specifically decomposed by aureus) to form a colony of cyan is read more of the detection results are given in the table below.

The detailed reading of detection result follows the table <9-1>.

Table 9-1

 Strain (ATCC)  Colony growth Rhamnose fermentation Color of colony  Listeria monocytogenes (L.monocytogenes) (19118)  Flourishing + (Yellow border: yellow halo)  Blue  Listeria Ivanovii (19119)  Flourishing   -  Blue  Listeria innocua (33090)  Flourishing + (Yellow border: yellow halo)  Blue  E. coli (25922)   -   -   -  B. subtilis (6633)   -   -   -  P. aeruginosa (27853)   -   -   -  Candida albicans (10231)   -   -   -

Example  10

The selective chromogenic medium by biochemical reactions synchronized with the selective culture used for culturing Staphylococcus aureus and then reading it is prepared as shown in Table 10.

TABLE 10

 Ingredients  Grams / Liter  Casein (Casein enzymic hydrolysate)  13.00  Yeast extract  2.50  Beef extract  2.50  Sodium pyruvate  5.00  Sodium chloride  40.00  Chromogenic mixture  5.30  Agar  15.00

After operating the detector and the detection kit in the same manner as in <Example 1>, the Staphylococcus detection is selected in the selection menu of the detector cap. Then, the culture reaction for 24 hours at 35 ~ 37 ℃ is automatically carried out by a detector, and then goes through the same process as in <Example 1>.

The chromogenic mixture of the components is specifically degraded by staphylococcus aureus to form a turquoise colony, and the detailed detection results are shown in the table below.

The detailed reading of detection result follows the table <10-1>.

Table 10-1

 Strain (ATCC)  Colony Growth  Color of colony  S. aureus (25923)  Flourishing  Bluish-green  Staphylococcus epidermidis (12228)  Flourishing  Bluish-green  E. coli (25922)  Poor-good  Purple  Enterobacter faecalis (29212)  Extremely poor (None-poor)  Light green  Staphylococcus maureus (6538)  Flourishing  Bluish-green  Staphylococcus xyloseus (29971)  Flourishing  Silver (Metallic)

Example  11

Selective coloring medium by biochemical reactions synchronized with the selective culture used to incubate Clostridium perfringens and then read it is added to <Table 11>.

TABLE 11

 Ingredients  Grams / Liter  Tryptose  30.00  Yeast extract  20.00  Sucrose  5.00  L-Cysteine hydrochloride  1.00  Magnesium sulphate (7H2O)  0.10  Bromo cresol purple  0.04  Ferric chloride (6H2O)  0.09  Chromogenic mixture  0.06  Agar  15.00

After the detector and the detection kit are operated in the same manner as in <Example 1>, detection of Clostrium perfringens is selected in the selection menu of the detector cap. Then, the culture reaction for 24 hours at 44 ℃ is automatically carried out by a detector, and then goes through the same process as in <Example 1>.

Indoxitadiggleindobetabetaglucosidase, which is used as a chromogenic mixture among the components, is a betadiglucosidase specific to Clostridium perfringens (βD-glucosidase). ) Or as a substrate of cellobiase to form a pale yellow colony.

The detailed reading of detection result follows the table <11-1>.

Table 11-1

 Strain (ATCC)  Colony Growth  Color of colony  Clostrodium perfringens (12924)  Good  Light yellow  Staphylococcus aureus (25923)  Inhibited   -  B. subtilis (6633)  Inhibited   -  S. serotype Typhi  Inhibited   -

Example  12

Selective color media by biochemical reactions synchronized with selective cultures used to read Bacillus species after culturing Bacillus species is added to <Table 12>.

TABLE 12

 Ingredients  Grams / Liter  Peptic digest of animal tissue  10.00  Meat extract  1.00  D-Mannitol  10.00  Sodium chloride  10.00  Phenolic red  0.025  Chromogenic mixture  3.20  Agar  15.00

After the detector and the detection kit are operated in the same manner as in Example 1, the bacterium detection is selected from the selection menu of the detector cap. Then, the culture reaction for 24 hours at 30 ℃ is automatically carried out by a detector, and then goes through the same process as in <Example 1>.

Phenol red (phenol red) of the composition as an indicator to see whether the mannitol (mannitol) fermentation colonies of the mannitol (mannitol) fermentation such as Bacillus megaterium (Bacillus megaterium) is yellow. In addition, colonies of bacteria producing beta glucosidase, such as Bacillus cereus, decompose chromogenic mixtures to form blue colonies.

The detailed reading of detection result follows the table <12-1>.

Table 12-1

Strains (ATCC)  Colony Growth  Color of colony  B. subtilis (6633)  None  Light green to green  B. cereus (10876)  Flourishing  Light blue, large, flat colonies  B. thuringiensis (10792)  Flourishing Light blue, large, flat colonies with irregular margins  Bacillus megaterium (B. megaterium) (14581)  None  Yellow (Yellow, mucoid)  B. coagulans (7050)  None  Pink, small, raised colonies  Staphylococcus aureus (25923)  Inhibited  Yellow  E. faecalis (29212)  Inhibited  Yellow

Example  13

Selective cultures used to read Vibrio species after cultivation and selective color media by biochemical reactions synchronized with them are added to <Table 13>.

TABLE 13

 Ingredients  Grams / Liter  Peptic digest of animal tissue  10.00  Sodium chloride)  25.00  Sodium thiosulphate  5.00  Sodium citrate  6.00  Sodium cholate  1.00  Chromogenic mixture  5.50  Agar  15.00

After operating the detector and the detection kit in the same manner as in Example 1, Vibriobacteria detection is selected from the selection menu of the detector cap. Then, the culture reaction for 24 hours at 35 ~ 37 ℃ is automatically carried out by a detector, and then goes through the same process as in <Example 1>.

The chromogenic mixture of the composition is decomposed by beta galactosidase of Vibrio bacteria, and the colony of Vibrio cholerae is purple, and the colony of Vibrio parahaemolyticus is purple. Dyed cyan.

The detailed reading of detection result follows the table <13-1>.

Table 13-1

 Strain (ATCC)  Growth of colonies  Color of colony  Vibrio cholera (15748)  Good-luxuriant  Purple  V. parahaemolyticus (17802)  Good-luxuriant  Bluish-green  Enterobacter pecalis (29212)  Inhibited   -  Staphylococcus aureus (25923)   -   -  E. coli (25922)  -  -

Example  14

The composition of the selective culturing medium by the selective culture used to measure the total number of microorganisms and the biochemical reactions synchronized therewith is shown in Table 14.

TABLE 14

 Ingredients  Grams / Liter  Peptone special  23.00  Yeast extract  2.00  Sodium chloride  5.00  Lecithin  1.00  Polysorbate 80  7.00  Dextrose  1.00  Sodium bisulphate  0.10  Indicator dye  0.10  Agar  15

Operate the detector and detection kit in the same manner as in <Example 1>, and then select anaerobic bacteria detection from the selection menu of the detector cap. After 24 hours at 35 ~ 37 ℃ culture reaction proceeds automatically by the detector.

E. coli and staphylococcus aureus form a red colony and detailed reading criteria are as follows.

The detailed reading of detection result follows the table <14-1>.

Table 14-1

 Strain (ATCC)  Colony Growth  Color of colony  E. coli (25922)  Flourishing  Pink to red  Staphylococcus aureus (25923)  Flourishing  Pink to red

Example  15

Selective cultures used for the detection of fungi (Yeast and mould) among the individual microorganisms and selective color medium by biochemical reactions synchronized to them are shown in Table 15.

TABLE 15

 Ingredients  Grams / Liter  Potato infusion  200.00  Dextrose  20.00  Indicator  0.03  Agar  15.00

After operating the detector and the detection kit in the same manner as in Example 1, detection of the fungus is selected from the selection menu of the detector keypad. After 48 hours at 22 ~ 28 ℃ culture is automatically carried out by the detector.

The detailed reading of detection result follows the table <15-1>.

Table 15-1

 Strain (ATCC)  Colony Growth  Color of colony  Candida albicans  Flourishing (Luxuriant)  Pink (pnkish white)  Staphylococcus aureus (S. cerevisiae)  Flourishing (Luxuriant)  Pink white

Example  16

Selective culture used in other methods of detecting coliform (Coliform) and selective color medium by biochemical reactions synchronized to it are prepared as shown in Table 16.

TABLE 16

 Ingredients  Grams / Liter  Peptic digest of animal tissue  7.00  Yeast extract  3.00  Lactose  10.00  Bile salt mixture  1.50  Sodium chloride  5.00  Indicator dye  0.03  Crystal violet  0.02  Agar  15.00

After the detector and the detection kit are operated in the same manner as in Example 1, the E. coli group detection is selected from the selection menu of the detector keypad. After 24 hours at 35 ~ 37 ℃ culture reaction proceeds automatically by the detector.

Among the colonies formed at this time, pink colonies are Enterobacter aerogenes, pink colonies having a ring around them are E. coli, and colorless colonies are staphylococcus serotype enteritidis. Therefore, the entire pink colony is read as E. coli colony.

The detailed reading of detection result follows the table <16-1>.

Table 16-1

 Strain (ATCC)  Colony Growth  Color of colony  Enterobacter erogenes (13048)  Flourishing  Pink  E. coli (25922)  Flourishing Red (Pinkish red with bile precipitate)  Staphylococcus (S. aureus) (25923)  Inhibited   -  Staphylococcus S. serotype Enteritidis (13076)  Flourishing  Colorless

Example  17

 Selective culture used to detect anaerobic microorganism and selective color medium by biochemical reactions synchronized to it are prepared as shown in <Table 17>.

TABLE 17

 Ingredients  Grams / Liter  Tryptose  15.00  Pancreatic digest of soybean meal  5.00  Sodium chloride  5.00  Indicator dye  0.10  Agar  15.00

Operate the detector and the detection kit in the same manner as in <Example 1>, and then select anaerobic bacteria detection from the selection menu of the detector keypad. After 24 hours at 35 ~ 37 ℃ culture reaction proceeds automatically by the detector.

Anaerobic bacteria, such as Bacillus subtilis, E. coli, and staphylococcus aureus, form red colonies and detailed reading criteria are as follows.

The detailed reading of detection result follows the table <17-1>.

Table 17-1

 Strain (ATCC)  Colony Growth  Color of colony  B. subtilis (6633)  Flourishing  Maroon (Red to maroon)  E. coli (25922)  Flourishing  Maroon (Red to maroon)  Enterobacter faecalis (29212)  Flourishing  Maroon (Red to maroon)  Staphylococcus aureus (25923)  Flourishing  Maroon (Red to maroon)  Staphylococcus pyrogenes (19615)  Flourishing  Maroon (Red to maroon)

Example  18

Selective cultures used to detect Staphylococcus aureus and selective color medium by biochemical reactions synchronized with them are prepared as shown in Table 18.

TABLE 18

 Ingredients  Grams / Liter  Proteose peptone  10.00  Beef extract  1.00  Sodium chloride  75.00  D-Mannitol  10.00  Indicator  0.025  Agar  15.00

After operating the detector and the detection kit in the same manner as in <Example 1>, the Staphylococcus detection is selected in the selection menu of the detector cap. After 24 hours at 35 ~ 37 ℃ culture reaction proceeds automatically by the detector.

The detailed reading of detection results follows the table <18-1>.

Table 18-1

 Strain (ATCC)  Colony Growth  Color of colony  E. coli (25922)  Inhibited  -  Staphylococcus aureus (25923)  Good-luxuriant  Yellow  Staphylococcus epidermidis  Fair-good  Red

Example  19

 Selective cultures used to detect Bacillus species and selective color medium by biochemical reactions synchronized to them are prepared as shown in Table 19.

 Ingredients  rams / Liter  Biopeptone  6.50  Yeast extract  1.50  Sodium chloride  5.00  Agar  15.00

Operate the detector and the detection kit in the same manner as in <Example 1>, and then select Bacillus bacteria detection from the selection menu of the detector cap. After 18 hours at 35 ~ 37 ℃ culture reaction is automatically proceeded by the detector.

The detailed reading of detection results follows the table <19-1>.

Table 19-1

 Strain (ATCC)  Growth of colonies  B. cereus (10876)  Luxuriant  B. subtilis (6633)  Luxuriant

Example  20

Selective culture used to detect microorganisms belonging to the coliform group (Coliform) performing lactose fermentation and selective color medium by biochemical reactions synchronized thereto are prepared as shown in <Table 20>.

TABLE 20

 Ingredients  Grams / Liter  Peptic digest of animal tissue  10.00  Lactose  10.00  Oxgall  20.00  Brilliant green  0.0133

Among the microorganisms belonging to the E. coli group, microorganisms performing lactose fermentation generate gas as a result of this metabolism. In the same manner as in <Example 1>, E. coli group detection is selected from the selection menu of the detector cap. After 24 hours at 35 ~ 37 ℃ culture reaction proceeds automatically by the detector.

However, this method uses a tube kit in the form of a test tube, so when placed in the culture chamber using a test tube mounting tray (tray) prepared inside the culture chamber to be safely positioned.

The reading of the result after the incubation is completed is based on the generation of bubbles due to gas generation in a broth inside the test tube. That is, if there is a bubble indicating gas generation, E. coli group is detected positively. If no bubble is observed, it is read as E. coli group negative.

As shown in the above examples, the present invention uses a selective color reaction medium by a biological reaction synchronized with a selective culture suitable for the detection of the target bacteria, and only by the previous complicated, expensive and professional by the automatic progress of the culture reaction, etc. The detection of live bacteria performed was simple, quick and non-expert.

 In addition, it is possible to apply a variety of detection technology by introducing a variety of auxiliary functions such as using a UV lamp and a tray to apply a variety of detection kits. For this reason, the detection method of the present invention can be used in various aspects from research purposes to microbial inspection of foods, as well as environmental hygiene and microbial safety-related inspection.

 In addition, these advantages of the present invention has the effect that can be used in homes and small businesses with the safety of its own sterilization function.

1 is a block diagram showing a live bacteria detection method of the present invention.

Figure 2a is a perspective view showing a live bacteria detection device of the present invention.

Figure 2b is a cross-sectional view showing a live bacteria detection device of the present invention.

Figure 3a is an exploded perspective view showing a detection kit of the live bacterial detection device of the present invention.

Figure 3b is a cross-sectional view showing a tray of the live bacteria detection device of the present invention.

Figure 4 is a block diagram of a biomicrobial detection apparatus according to an embodiment of the present invention.

5 is a flow chart of the operation of the biodetection apparatus according to an embodiment of the present invention.

6 is a flow chart of the culture unit driving operation according to an embodiment of the present invention.

Explanation of symbols on the main parts of the drawings

100: detector

110: chamber 111: culture chamber

112: locking groove 120: cover

121: outer cover 122: inner cover

130: detection kit 131: inner container

131a: smear groove 131b: batch projection

132: outer container 132a: storage space

133: connecting member 140: culture unit

141: fan module 142: heat generating module

150: reading unit 160: sterilization unit

161: sterilization indicator 170: controller

171: keypad 172: memory

173: timer unit 174: audio processing unit

175: control unit 176: image processing unit

177: display unit 178: temperature sensor unit

180: tray 181: settle space

182: locking jaw 183: latch

184: partition bulkhead

Claims (2)

Manufacturing a detection kit in which a detection kit inoculated with a detection medium in which a selective growth of a target bacterium by a selective culture component and a biochemical reaction by a selective chromogenic substrate are inoculated into a triple sealed structure; A sample inoculation step of selecting one of three types of inoculation of dilution, smearing, and surface contact to inoculate the detection medium; The detection medium inoculated with the sample is placed inside the detector to satisfy the culture conditions such as the incubation temperature and incubation time suitable for the detection purpose, and the detector is pre-incubated with the culture conditions suitable for the purpose of detecting the target bacteria. There is a culturing step to automatically set the culture conditions suitable for detection by a simple selection operation through the operation unit of the detector, A detection result reading step of visually observing a color change of the colony of detection target bacteria in the detector to confirm the result; Through the sterilization indicator mounted inside the detector, the discoloration or discoloration reaction is confirmed in response to ozone at a specified concentration to identify sterilization conditions in the incubation chamber. A method for detecting live bacteria, in which selective growth and biochemical reactions are synchronized. A chamber formed inside the culture chamber with an upper opening; A cover part composed of an opaque outer cover and a transparent inner cover that are hinged in multiple stages to be coupled to a hinge to one side of the culture chamber so as to be opened and closed to provide a dark room culture condition in the culture chamber, if necessary; A detection kit which is placed inside the culture chamber by receiving a selective culture component suitable for the detection target bacterium and a detection medium synchronizing a biochemical reaction by a selective color substrate, A heating module is provided at the outside of the chamber and an external air is introduced into the culture chamber through the heater wire from the fan module mounted therein, thereby maintaining the culture temperature of the target bacteria in the culture chamber in accordance with the culture condition time. , Mounted inside the culture chamber to generate ozone to sterilize the bacteria read after the cultivation, and installed outside the chamber to control the heating module, lamp module, sterilization to meet the culture conditions of the target bacteria to perform sterilization A live bacterial detection device comprising a controller unit which allows the selective growth and biochemical reactions to be synchronized.

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