KR101961658B1 - Apparatus for detecting a microbe - Google Patents

Abstract

TECHNICAL FIELD The present invention relates to a mobile type microorganism detection device which can be detected by culturing microorganisms. According to an embodiment of the present invention, there is provided a food processing apparatus comprising: a housing member including an outer container and an inner container positioned inside the outer container and having a culture space therein for culturing the microorganisms therein; A temperature control member for controlling the internal temperature of the microorganism to be cultivated in the internal space of the receiving member, and a temperature control member for controlling the internal temperature of the micro- And an inspection member for inspecting whether or not a specific microorganism is included in the microorganisms cultured after the set incubation time.

Description

[0001] Apparatus for detecting a microbe [0002]

The present invention relates to a mobile microorganism detection device, and more particularly, to a mobile microorganism detection device capable of quickly and easily detecting microorganisms in a sample.

Microbial infections are a major pathway for the development of human and animal diseases, resulting in serious clinical and economic adverse effects. Particularly, Escherichia coli contained in food, drinking water or food among these microorganisms induces diseases such as food poisoning in humans and causes a lot of economic loss.

Escherichia coli / Escherichia coli is a hygienic indicator bacterium and is known to be an indicator of fecal contamination in particular. Since it is practically difficult to individually examine pathogenic microorganisms, the overall phase status can be confirmed by examining the hygienic indicator bacteria. 10% of Escherichia coli is pathogenic Escherichia coli, and the number of cases of food poisoning caused by it is 39, 2123 in 2015, which is the highest rate of food poisoning. Conventional methods for inspecting E. coli include enrichment and isolation and identification in a selective medium, which takes about 4 days. Conventional E. coli assays based on these biochemical methods are time-consuming and require expertise.

In recent years, there has been an increasing tendency to use an enzyme analysis method for the detection of E. coli. Enzymatic method is a technique that uses the principle that E. coli secretes β-D-galactosidase and β-D-glucuronidase to decompose the coloring substrate of the reagent used in the enzyme coloring method, causing color change and showing fluorescence. These enzymatic method is an official method listed in the Ministry of Environment, EPA and AOAC, and it can quickly check the presence of E. coli in 18 hours.

However, the enzyme coloring method is a method requiring special equipment such as an incubation, a UV apparatus and a high pressure sterilizer, and has a limitation in application to a home, a foodservice or an agriculture industry field. Therefore, it is necessary to develop a method capable of easily and effectively detecting food poisoning bacteria in the living environment as described above.

In addition, the apparatus for culturing the microorganisms collected from the sample and the apparatus for detecting the cultured microorganisms are performed in different apparatuses. However, in this case, it takes much time and effort to detect microorganisms.

An object of the present invention is to provide a mobile type microorganism detecting device which can easily detect and detect microorganisms in a daily life environment such as a home, a foodservice, or an agricultural product industry field.

The present invention also provides a portable microorganism detecting device which can be detected by culturing microorganisms by various methods.

The present invention also provides a portable microorganism detection apparatus capable of performing culture and detection of microorganisms in one apparatus.

The present invention is not limited thereto, and other objects not mentioned may be clearly understood by those skilled in the art from the following description.

The present invention provides an apparatus that can be detected by culturing microorganisms.

According to an embodiment of the present invention, a portable microorganism detection device includes a housing member including an outer container and an inner container positioned inside the outer container and having a culture space for culturing the microorganisms therein, A support member which is divided into a plurality of spaces so that the microorganisms are cultured from the specimens collected in different ways in the internal space of the housing member and a temperature control unit for adjusting the internal temperature so that the microorganisms are cultured in the internal space of the accommodation member An adjusting member and an inspection member for inspecting whether or not a specific microorganism is included in the microorganism cultured after the predetermined incubation time.

According to one embodiment, the support member includes a first support portion on which the first specimen is placed and a second specimen different from the first specimen, a second support portion adjacent to the first support portion, A third sample different from the second sample may be placed, and the third support portion may be adjacent to the first support portion and the second support portion.

According to an embodiment, the first support portion includes a first plate having a plurality of first holes, and the second support portion includes a second plate having a plurality of second holes, The number and size of the two holes may be provided differently.

According to one embodiment, the first sample or the second sample is cultured in a state of being accommodated in a closed container and inserted into the first hole and the second hole, respectively, and the third sample is accommodated in the dry film And may be placed in the inner space of the third support.

According to one embodiment, the temperature regulating member may include a heating unit for heating the inner container, a temperature sensing unit installed inside the outer container for sensing a temperature, and an internal temperature sensor And a temperature controller for receiving the information and controlling the internal temperature.

According to one embodiment, the heating unit may be provided with a silicone heater disposed around the inner container.

According to one embodiment, the temperature regulating member is disposed at a lower portion of the outer container, and includes a heat transfer fluid supply portion for supplying a heat transfer fluid, which is a fluid heated or cooled, in the outer container, And a temperature controller for receiving the internal temperature information of the internal container sensed by the temperature sensor and controlling the internal temperature.

According to one embodiment, the heat transfer fluid supply unit may include a thermoelectric element to heat or cool the heat transfer fluid.

According to one embodiment, the temperature regulating member may further include a lower plate provided at a lower portion of the outer container and having a fluid hole through which the heat transfer fluid can pass.

According to an embodiment, the temperature regulating member may further include a blowing unit formed at a lower portion of the lower plate and supplying the heat transfer fluid heated or cooled by the heat transfer fluid supply unit to the inside of the outer container.

According to one embodiment, the temperature control unit controls the heat transfer fluid supply unit to heat or cool the heat transfer fluid so as to maintain the temperature of the heat transfer fluid at a predetermined temperature based on the internal temperature information received from the temperature sensing unit. can do.

According to one embodiment, the temperature sensing part may be located apart from the inner wall of the outer container.

According to an embodiment of the present invention, the inspection member may include a light source unit located inside the outer container, a light source control unit for controlling whether the light source unit emits light or not, and a state after the light irradiated from the light source unit passes through the sample And an observation section located in the outer vessel for observation.

According to one embodiment, the observation section may include an observation hole located at one side wall of the outer vessel and an observation cover coupled to one side wall of the outer vessel to cover or open the observation hole.

According to one embodiment, the light source unit is located outside the inner container, and may be installed on the inner wall of the outer container.

According to one embodiment, the light source is provided as a UV lamp, and the illuminance may be 800 lux or more.

According to one embodiment, the inner vessel may be provided with a transparent material.

According to one embodiment, a plurality of exhaust holes may be formed on one side of the outer container to allow the fluid inside and the outside air to flow.

According to an embodiment of the present invention, microorganisms can be cultured and detected through a sample collected at various sites, so that microorganisms can be detected quickly and easily.

Further, according to the embodiment of the present invention, the culture and detection of the microorganism can be performed at the same time, and the microorganism detection efficiency can be improved.

In addition, according to an embodiment of the present invention, the portable microorganism detection device can be connected to a power source device of a vehicle during transportation of the vehicle to cultivate the microorganism, thereby shortening the experiment time.

The effects of the present invention are not limited to the above-mentioned effects, and the effects not mentioned can be clearly understood by those skilled in the art from the present specification and attached drawings.

1 is a perspective view showing a mobile type microorganism detecting apparatus according to an embodiment of the present invention.
2 is an exploded perspective view showing a mobile type microorganism detecting apparatus according to an embodiment of the present invention.
Figs. 3 and 4 are side views showing the mobile type microorganism detecting apparatus of Fig. 1. Fig.
5 is a cross-sectional view showing a mobile type microorganism detecting apparatus according to an embodiment of the present invention.
FIG. 6 is a cross-sectional view showing a part of the interior of the outer container of FIG. 1;
FIG. 7 is a perspective view showing a state in which a sample is housed in the support member of FIG. 2. FIG.
8 is an exploded perspective view showing a mobile type microorganism detecting apparatus according to another embodiment of the present invention.
9 is a cross-sectional view showing a part of the mobile type microbial detection device of FIG.
FIG. 10 and FIG. 11 are views showing a process of an experimental example according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The embodiments of the present invention can be modified in various forms, and the scope of the present invention should not be construed as being limited to the following embodiments. This embodiment is provided to more fully describe the present invention to those skilled in the art. Accordingly, the shape of the elements in the figures may be exaggerated in order to emphasize a clearer description. In addition, terms and words used in the present specification and claims should not be construed as limited to ordinary or dictionary meanings, and the inventor should appropriately define the concept of the term to describe its invention in the best way possible. It should be construed as meaning and concept consistent with the technical idea of the present invention.

The portable microorganism detecting apparatus (10) of the present invention is a device capable of detecting microorganisms in a daily living environment such as a home, a foodservice station, a restaurant, or an agro-food industry field. The microorganisms of the present invention include not only pathogenic Escherichia coli but also microorganisms capable of causing diseases in humans.

The portable microorganism detecting apparatus 10 of the present invention can collect samples in various environments such as workers, work tools, workers' gloves, agricultural products, seeds, agricultural water, and drinking water. The mobile type microorganism detecting apparatus 10 of the present invention can be cultured in the mobile type microorganism detecting apparatus 10 of the present invention by inserting the collected sample into a culture container or a dry film suitable for the target microorganism. The mobile type microorganism detecting apparatus (10) of the present invention can detect a target microorganism to be detected by using light.

FIG. 1 is a perspective view showing a mobile-type microorganism detection device 10 according to an embodiment of the present invention, FIG. 2 is an exploded perspective view showing a mobile-type microorganism detection device 10 according to an embodiment of the present invention, FIG. 4 is a side view of the mobile type microorganism detection device 10 of FIG. 1, FIG. 5 is a cross-sectional view of the mobile type microorganism detection device 10 according to an embodiment of the present invention, Sectional view showing part of the interior.

1 to 6, the mobile type microorganism detection apparatus 10 includes a housing member 100, a support member 200, a temperature control member 300, and an inspection member 400.

Hereinafter, the direction in which the handle 113 and the outer container 110 are arranged side by side is referred to as a first direction X. [ A direction perpendicular to the first direction X is referred to as a second direction Y when viewed from above. A direction perpendicular to both the first direction (X) and the second direction (Y) is referred to as a third direction (Z).

The housing member 100 has a space therein. A space in which the microorganisms are cultured can be provided in the inner space of the housing member 100.

The housing member 100 includes an outer container 110 and an inner container 130.

The outer container 110 may be provided in an open top form. The outer container 110 may have a generally rectangular parallelepiped shape. The outer container 110 may be made of a plastic material. For example, the outer container 110 may be provided with a PE material.

An exhaust hole 151 may be formed on one side of the outer container 110. The exhaust hole 151 may be formed to allow fluid to flow inside or outside the outer container 110. A plurality of exhaust holes 151 may be formed on one side of the outer container 110. The plurality of exhaust holes 151 may be spaced a certain distance from one side of the outer container 110. The exhaust hole 151 may be provided to circulate the outside air and the inside air when the microorganism is cultured to supply oxygen necessary for culturing the microorganism.

The outer cover 111 can be engaged with the outer container 110. The outer cover 111 can cover the upper portion of the outer container 110. The outer cover 111 may be located at an upper portion of the outer container 110 in the third direction Z. [ For example, the outer cover 111 may be combined with the outer container 110 to open or close the upper portion of the outer container 110. [ For example, when the sample collected in the inner space of the housing member 100 is inserted, the upper portion of the outer container 110 may be opened using the outer cover 111. [ Alternatively, when the microorganisms contained in the sample collected in the internal space of the housing member 100 are cultured or microorganisms are detected, the upper portion of the outer container 110 may be covered with the outer cover 111.

A handle 113 may be coupled to one side of the outer container 110. A pair of the grip portions 113 may be provided. For example, one pair of the grip portions 113 may be coupled to opposite sides of the outer container 110 facing each other in the first direction (X). The handle 113 can be provided in a shape such that the operator can hold the mobile microorganism detecting device 10 with both hands when moving. For example, the handle 113 may be formed in a U-shape.

The inner container 130 may be provided in an open top form. The inner container 130 may have a rectangular parallelepiped shape as a whole. A support member 200 to be described later may be disposed inside the inner container 130. The inner vessel 130 may be provided with a transparent material. As an example, the inner container 130 may be made of a plastic material.

The inner vessel 130 is provided with a transparent material so that the presence of the microorganism to be detected can be confirmed through the light passing through the inner vessel 130 during the inspection with the inspection member 400 described later.

The inner cover 131 may cover the upper portion of the inner container 130. The inner cover 131 can open the upper portion of the inner container 130 when the sample is taken in. Alternatively, the inner cover 131 may cover the upper portion of the inner container 130 when the microorganisms are cultured or microorganisms are detected.

The support member 200 can store or support the sample therein. The support member 200 can be partitioned into a plurality of spaces so that the microorganisms can be cultured from the specimens collected in various ways.

The support member 200 includes a first support portion 210, a second support portion 230, and a third support portion 250.

The first support part 210 can support a container containing the first sample S1. A first sample S1 may be placed on the first support portion 210. For example, the first support portion 210 may be provided with a first sample S1 contained in a cylindrical test tube vessel.

The first support portion 210 may include a first plate 211. The first plate 211 may be located in the inner space of the first support portion 210. The first plate 211 may be made of a transparent material. The first plate 211 may be made of a plastic material. The first plate 211 may have a plurality of first holes 213 formed therein.

As shown in FIG. 7, the first sample S1 contained in the test tube vessel may be inserted into the first hole 213. The plurality of first holes 213 may be spaced apart from each other by a predetermined distance.

The second support portion 230 may be positioned adjacent to the first support portion 210. The first support portion 210 and the second support portion 230 may be arranged in parallel along the second direction Y. [ The second support part 230 can support the container containing the second sample S2. As an example, the second support portion 230 may be provided with a second sample S2 contained in a generally cylindrical container. Here, the container containing the second sample S2 may be a container larger than the container containing the first sample S1.

The second support portion 230 may have a second plate 231. The second plate 231 may be located in the inner space of the second support portion 230. The second plate 231 may be made of a plastic material. The second plate 231 may have a plurality of second holes 233 formed therein.

The second sample S2 contained in the cylindrical container can be inserted into the second hole 233 as shown in FIG. The plurality of second holes 233 may be spaced apart from each other by a predetermined distance. The second hole 233 may be provided in a larger size than the first hole 213. The second holes 233 may be provided in a smaller number than the first holes 213.

The third support portion 250 may be positioned adjacent to the first support portion 210 and the second support portion 230. The third support portion 250 may be disposed in parallel with the first support portion 210 and the second support portion 230 along the first direction X. [ The third support part 250 may have a space in which the third sample S3 is placed. As an example, the third sample S3 may be a dry film. As shown in FIG. 7, the dried film may be positioned in the inner space of the third support part 250 in a sealed form after the sample containing the microorganism is buried therein. For example, a plurality of dry films may be placed on the third support portion 250, and the microorganism may be cultured in the third sample S3.

The first sample (S1), the second sample (S2) and the third sample (S3) can be collected by different methods, respectively. For example, the first sample (S1) and the second sample (S2) can be stored in a test tube or a container by mixing a mixture of a sample collected in various environments and a pretreatment solution.

The third sample (S3) can be stored in a state in which the collected sample is accommodated in the dry film.

The temperature regulating member 300 can regulate the temperature inside the housing member 100 to cultivate microorganisms of the sample. The temperature regulating member 300 can adjust the temperature inside the housing member 100 to an appropriate temperature at which the microorganism can be cultured.

The temperature regulating member 300 includes a heating unit 310, a temperature sensing unit 330, and a temperature control unit 350.

The heating unit 310 can heat the inner vessel 130. The heating unit 310 may be provided to surround the inner vessel 130. For example, the heating unit 310 may be provided with a silicone heater disposed around the inner container 130. Alternatively, the heating unit 310 may be provided in the form of a heating coil. Unlike the above-described example, the heating unit 310 is not limited as long as it is an apparatus for heating the inner vessel 130 by a known heating apparatus.

The temperature sensing unit 330 may be installed inside the outer container 110. The temperature sensing unit 330 is installed inside the outer container 110 to check the incubation temperature of the microorganism in real time. For example, the temperature sensing unit 330 may be installed at the bottom of the outer container 110. The temperature sensing unit 330 may be installed apart from the inner wall of the outer container 110. The temperature sensing unit 330 is spaced apart from the inner wall, so that the internal temperature of the outer container 110 can be more accurately sensed. For example, the temperature sensing unit 330 may be provided as a temperature sensor to measure the internal temperature of the outer container 110.

The temperature controller 350 may determine the internal temperature information transmitted from the temperature sensor 330 to control the internal temperature of the external container 110. Specifically, the temperature control unit 350 can heat the heating unit 310 by operating the internal temperature of the external container 110 when the internal temperature of the external container 110 is lowered, on the basis of the set temperature required for culturing the microorganism. The operation of the heating unit 310 may be stopped when the internal temperature of the external container 110 rises above the set temperature and the operation of the heating unit 310 may be stopped so as to maintain the internal temperature at an appropriate temperature.

The temperature control unit 350 may be installed on one side of the outer container 110. For example, the temperature control unit 350 may have a display unit and an operation unit. The display unit may display the internal temperature sensed by the temperature sensing unit 330. The operating part can determine the set temperature value of the internal temperature.

The inspection member 400 can check whether the microorganism cultured through the sample contains a specific microorganism or a microorganism to be examined. For example, the inspection member 400 may use light to inspect for specific microorganisms.

The inspection member 400 includes a light source unit 410, a light source control unit 430, and an observation unit 450.

The light source unit 410 can irradiate the container or the film having the sample with light. The light source part 410 may be located inside the outer container 110. For example, the light source unit 410 has a plurality of lamps and may be installed in three stages on one side wall of the outer container 110. For example, the light source unit 410 may be installed in three stages on inner walls of the outer container 110 facing each other.

As an example, the lamp of the light source unit 410 may be provided as a UV lamp. Specifically, the lamp of the light source unit 410 may be provided as a UV lamp of 365 nm. The lamp may be provided as an LED lamp. The illuminance of light emitted from the light source unit 410 may be 800 lux (LUX) or more.

The light source control unit 430 can control the light emitting unit 410 to emit light. For example, the light source control unit 430 can control the light of the light source unit 410 in an ON / OFF manner. For example, the light source control unit 430 may control the light source of the light source unit 410 of the external container 110 to emit light through a switch provided on an outer wall of the external container 110.

The observation unit 450 can observe the state after the light irradiated from the light source unit 410 has passed through the cultured sample. The observation part 450 may be located in the outer vessel 110. In one example, the observation section 450 may be provided on one side of the outer vessel 110. A plurality of observation portions 450 may be provided. For example, the observation unit 450 may be disposed on both sides of the outer container 110 facing each other.

The observation section 450 includes an observation hole 451 and an observation cover 453. [

The observation hole 451 may be located on one side wall of the outer vessel 110. The observation hole 451 may be formed in an elliptical shape whose cross section is long. The observation hole 451 may be formed at a lower height than the light source unit 410 installed in the outer container 110.

The observation cover 453 can cover or open the observation hole 451. [ The observation cover 453 may be coupled to one side wall of the outer container 110. In one example, the observation cover 453 may be hingedly connected to the outer container 110. The observation cover 453 can cover the observation hole 451 when the microorganism is cultured in the sample. Accordingly, the inside of the outer container 110 can be sealed. Alternatively, the observation cover 453 may move to the upper portion of the observation hole 451 when inspecting the presence of microorganisms.

According to an embodiment of the present invention, the mobile microorganism detection device 1 can be connected to a power source device of a vehicle during the transportation of a vehicle to cultivate microorganisms. For example, the mobile microorganism detection device 1 can be operated in connection with a cigar jack of a vehicle. Through this, the experiment time of microorganism culture can be shortened.

Hereinafter, a moving type microorganism detecting apparatus 10a according to another embodiment of the present invention will be described.

FIG. 8 is an exploded perspective view showing a moving type microorganism detecting apparatus according to another embodiment of the present invention, and FIG. 9 is a sectional view showing a part of the moving type microorganism detecting apparatus of FIG.

8 and 9, the mobile microbial detection device 10a of FIG. 8 is provided substantially the same as the mobile microbial detection device 10 of FIG. 1, but the temperature regulating member 800a is different.

Hereinafter, with reference to Figs. 8 and 9, the temperature regulating member 800a will be described.

The temperature control member 800a includes a thermal fluid supply unit 811a, a lower plate 870a, a blowing unit 890a, a temperature sensing unit 830a, and a temperature control unit 850a.

The thermal fluid supply portion 811a can supply the heat transfer fluid to the inside of the outer container 110. [ As an example, the heat fluid may be provided as air. As an example, the heat transfer fluid may be a fluid heated or cooled to a predetermined temperature. Here, the predetermined temperature may be a predetermined temperature based on the optimum culture temperature set according to the type of the microorganism and the temperature inside the outer container 110 sensed by the temperature sensing unit 830a.

For example, when the internal temperature of the outer container 110 is higher than the optimum temperature of the microorganism, the preset temperature may be set to be lower than the optimum culture temperature. Alternatively, if the internal temperature of the outer container 110 is lower than the optimum temperature of the microorganism, the predetermined temperature may be set to be higher than the optimum culture temperature. Alternatively, the predetermined temperature may be a temperature equal to the optimum culture temperature when the optimum incubation temperature and the internal temperature of the outer vessel 110 are the same.

The thermal fluid supply 811a may be located in the outer vessel 110. In one example, the thermal fluid supply portion 811a may be located below the lower plate 870a. In one example, the thermal fluid supply portion 811a may include a thermoelectric element to heat or cool the thermal fluid. In one example, the thermoelectric element may be provided as a Peltier element. Alternatively, the thermal fluid supply 811a may be provided as a known device capable of heating or cooling the thermal fluid, but is not limited thereto.

The lower plate 870a may be disposed inside the outer container 110. [ The inner container 130 may be positioned on the upper surface of the lower plate 870a. The lower plate 870a may be located above the thermal fluid supply 811a. A fluid hole 871a may be formed in the lower plate 870a.

The fluid holes 871a can be supplied to the inside of the outer vessel 110 through the thermal fluid heated or cooled by the thermal fluid supply unit 811a. A plurality of fluid holes 871a may be provided on the lower plate 870a. The fluid hole 871a may be provided to be positioned between the inner container 130 and the inner wall of the outer container 110.

The blowing-in portion 890a is located inside the outer vessel 110. The blowing portion 890a may be disposed below the lower plate 870a. The blowing unit 890a can supply the heat fluid held at the predetermined temperature in the heat fluid supply unit 811a to the inside of the outer container 110. [ For example, the blowing unit 890a may be provided as an apparatus having a plurality of fans. For example, the operation of the plurality of fans can supply the heat fluid through the fluid hole 871a of the lower plate 870a. The heat fluid may be heated or cooled through the heat fluid supply portion 811a, and the heat fluid may be introduced into the interior of the outer container 110 at a predetermined temperature state.

The temperature sensing unit 830a may be installed inside the outer container 110 to check the incubation temperature of the microorganism in real time. The temperature sensing unit 830a may be installed to be spaced apart from the inner wall of the outer container 110. For example, the temperature sensing unit 830a may be installed on the lower plate 870a. For example, the temperature sensing unit 830a may be provided as a temperature sensor to measure the internal temperature of the outer container 110. [

The temperature control unit 850a may determine the internal temperature information transmitted from the temperature sensing unit 830a to control the internal temperature of the external container 110. [ Specifically, the temperature control unit 850a can heat the thermal fluid by operating the thermal fluid supply unit 811a when the internal temperature of the outer container 110 is lowered on the basis of the set temperature required for culturing the microorganism.

Specifically, the temperature control unit 850a can heat the thermal fluid through the thermal fluid supply unit 811a and supply the heated fluid to the inside of the outer container 110 when the inner temperature of the outer container 110 is lower than a proper incubation temperature.

Alternatively, when the internal temperature of the outer container 110 rises, the thermal fluid supply unit 811a may be operated to cool the thermal fluid.

Specifically, the temperature control unit 850a can cool the thermal fluid through the thermal fluid supply unit 811a and supply it to the inside of the outer container 110 when the inner temperature of the outer container 110 is higher than the proper incubation temperature.

The temperature controller 850a may be installed on one side of the outer container 110. [ For example, the temperature control unit 850a may have a display unit and an operation unit. The display unit may display the internal temperature sensed by the temperature sensing unit 830a. The operating part can determine the set temperature value of the internal temperature.

In the above-described embodiment, the internal temperature of the external container 110 can be sensed in real time, and the heated or cooled thermal fluid can be supplied, so that the internal temperature of the mobile microorganism detection device 10a can be stably maintained. In addition, it is possible to prevent the internal temperature from being overheated or overcooled, thereby inducing stable culture of microorganisms.

Hereinafter, the operation of the mobile microorganism detection device 10 of the present invention will be described.

The portable microorganism detection device (10) of the present invention can perform the culture and inspection of the microorganism at one time. For example, when the microorganism is cultured with the mobile microorganism detection device 10 of the present invention, a sample of the microorganism to be detected is collected and stored in a container or a dry film. Thereafter, the sample is placed in any one of several partitioned spaces of the support member 200, and the microorganism is cultured through the temperature regulating member 300. The incubation time of the microorganism can be set differently depending on the kind of the microorganism.

After the cultivation of the microorganism is finished, the microorganisms capable of detecting the microorganism can be detected using the enzyme coloring method or light, the light is irradiated to the sample using the light source unit 410 of the inspection member 400, It is possible to observe through the observation unit 450 whether or not there is a specific microorganism.

That is, the apparatus 10 for detecting a mobile microorganism of the present invention is a device capable of detecting microorganisms by culturing microorganisms in a sample. Accordingly, it is possible to perform the culture and inspection of the microorganism in one apparatus, not in a separate apparatus, so that the detection efficiency of the microorganism can be improved.

In addition, there is an effect that the detection of pathogenic Escherichia coli and Escherichia coli that cause the middle ear in the microorganism can be carried out quickly and easily.

In particular, it is possible to collect samples from various environments such as agriculture water, food processing field, home, foodservice, and restaurant, and to perform cultivation and detection of microorganisms simultaneously, thereby protecting humans from disease- .

Hereinafter, a method for detecting microorganisms using the mobile-type microorganism detecting apparatus 10 of the present invention will be described.

The method for detecting microorganisms according to an embodiment of the present invention includes a sample preparing step, a coloring substrate adding step, a culturing step, and an inspecting step.

The microorganism detection method of the present invention can detect microorganisms detected in various environments using the mobile microorganism detection apparatus (10).

In the sample preparation stage, samples can be prepared by putting samples collected in various environments into a test tube vessel, a cylindrical vessel or a dry film.

The collected sample can be put together with the pretreatment solution when it is put into a container or a dry film. The amount of the pretreatment solution can be varied depending on the kind of the sample. Also. The type of sample and the container used may vary depending on the pretreatment solution. As an example, the pretreatment solution used may be sterile distilled water.

Containers containing samples may be provided in sterile bottles, sterilized packs, conical tubes or test tubes.

Specifically, when a sample is taken from a working tool, the entire surface of the working tool can be rubbed with a sterilizing sponge to collect the sample in the sample preparation step. After that, sterilized distilled water may be mixed to contain the sample in a conical tube or a test tube.

If the sample is taken from the operator's hand, the sample can be collected by rinsing the hand with sterile distilled water after putting the hand into the sterilization pack. Samples contained in sterile distilled water can be prepared in a test tube, a container or a conical tube.

When collecting samples from gloves, add a certain amount of sterilized distilled water to sterilized packs, and put gloves on gloves to wash the gloves with sterilized distilled water. Samples contained in sterilized distilled water can be prepared in test tubes, vessels or conical tubes.

When collecting samples from agricultural products or seeds, prepare sterilized packs or other containers and add sterilized distilled water, which is a pretreatment solution, to the container in a certain amount. Thereafter, the vessel is shaken to homogenize the sample contained in agricultural products or seeds so as to be contained in sterilized distilled water. After that, the sample contained in the sterilized distilled water can be prepared in a separate test tube, container or conical tube.

The step of adding the chromogenic substrate is a step of adding a chromogenic substrate to the pretreatment solution containing the sampled sample. For example, the chromogenic substrate may be Colilert 18. Alternatively, the chromogenic substrate may be added with another chromogenic substrate depending on the type of the sample or microorganism.

The amount of the chromogenic substrate can be varied depending on the kind of the pretreatment solution and the kind of the microorganism.

In the culturing step, the sample can be put into a portable microorganism detecting apparatus 10 according to an embodiment of the present invention and cultured. The incubation time may vary depending on the type of microorganism. In this example, the incubation time may be set to a time selected from 12 hours to 48 hours depending on the type of the microorganism.

The temperature at the time of culturing may be set differently depending on the type of the microorganism. In one example, the incubation temperature can be set within the range of 30 to 44 占 폚.

The apparatus 10 for detecting a mobile microorganism according to an embodiment of the present invention may include a temperature regulating member 300 capable of regulating a culture space and a culture temperature therein to perform microbial culture.

The test step is a step of detecting microorganisms after the microorganism cultivation in the sample is finished. In the inspection step, it is possible to perform qualitative inspection for checking the presence of microorganisms and quantitative inspection for checking the number of microorganisms.

For example, in the case of qualitative inspection, the presence or absence of a microorganism can be determined depending on whether specific light or fluorescence is detected in the light passing through the sample by irradiating light to a sample container or a test tube containing the cultured microorganism.

For example, in the mobile type microorganism detection apparatus 10 according to an embodiment of the present invention, the presence or absence of microorganisms can be confirmed through the light passing through the observation unit 450 through the inspection member 400 described above.

On the other hand, when performing the quantitative inspection of microorganisms, it can be confirmed whether there is a certain number or more of the microorganisms depending on whether the color of the microorganism cultured in the dry film or the like is discolored or not.

Alternatively, as shown in the figure, a specimen may be cultured for each size in a separate portion of the pretreatment solution contained in the sample, and then irradiated with the light.

Specifically, the support plate can be divided into three zones in total. In the first zone, the pretreatment solution containing a plurality of samples is placed at a predetermined interval and then sealed. In the second zone, a larger amount of the pretreatment solution than the first zone is spaced apart at a predetermined interval and then sealed and stored. In the third zone, the remaining pretreatment solution can be sealed and stored for collective storage.

Thereafter, a support plate sealing and storing the pretreatment solution may be placed on the inner vessel 130 and then cultured.

The cultivated support plate is then irradiated with light to check the number of individual luminescence in the first zone and the second zone to confirm the number of microorganisms.

The microorganism detection method may further include a sterilization step.

The sterilization step is a step of sterilizing the microorganisms inside by adding purified tablets to the pretreated solution after the inspection. Depending on the amount of pretreatment solution and the type of microorganism, the amount of pellets added can be controlled.

As described above, the mobile-type microorganism detecting apparatus 10 according to an embodiment of the present invention can simultaneously perform culture and microorganism testing. In addition to the test for checking whether a microorganism exists, It is possible to perform the inspection simultaneously on one device, as opposed to using a conventional apparatus (a microorganism incubator, a light irradiation apparatus, and an inspection apparatus), and the inspection efficiency of the microorganism can be improved. In addition, the manufacturing cost of the apparatus is reduced compared to the conventional apparatus, which is economical in terms of manufacturing cost.

Hereinafter, experimental examples of the method for detecting microorganisms according to an embodiment of the present invention will be described.

FIG. 8 and FIG. 9 are views showing a process of an experimental example according to an embodiment of the present invention.

Referring to FIG. 8, the sample is firstly sampled. Sampling is carried out in the same manner as the microorganism detection method described above, depending on the environment and the object to be sampled. The reagent added to the sample can be added to the amount of each sample.

The collected sample is put into the mobile type microorganism detection device 1 and cultured. The mobile type microorganism detection device 1 can cultivate a microorganism in a sample. For example, the incubation temperature of the microorganism can be set at 37 캜. The incubation time can be from 12 hours to 18 hours. Alternatively, the incubation temperature and incubation time may be set differently depending on the type of sample.

The microorganism cultured in the sample can be detected using the mobile microorganism detection device 1 of the present invention. For example, the light source unit 410 irradiates the sample with light, and the presence or absence of the microorganism can be confirmed through the observation unit 450.

Referring to Fig. 9, the microorganism can be detected by another method. For example, the number of microorganisms can be confirmed in addition to the presence or absence of microorganisms. For example, the microorganism may be E. coli or E. coli. Hereinafter, a method for confirming the coliform count / coliform count will be described.

First dilute the sample. Sampling is carried out in the same manner as the microorganism detection method described above, depending on the environment and the object to be sampled. The sampled samples can be taken at a diluted concentration by a predetermined amount and then inoculated on a dry film. As an example, the amount of sample may be 1 ml. Alternatively, the amount of the sample contained in the dry film may be set differently depending on the type of the microorganism.

The dry film having microorganisms can be placed inside the support member 200 of the mobile type microorganism detection device 1. [

Thereafter, the microorganism can be cultured by using the mobile microorganism detection device (1). As an example, the incubation temperature of the microorganism may be 35 to 37 占 폚. As an example, the incubation time of the microorganism may be 24 hours. Alternatively, the incubation time and temperature of the microorganism may vary depending on the type of microorganism.

After the cultivation of the microorganism, the number of the microorganisms can be confirmed through the dry film.

The foregoing detailed description is illustrative of the present invention. In addition, the foregoing is intended to illustrate and explain the preferred embodiments of the present invention, and the present invention may be used in various other combinations, modifications, and environments. That is, it is possible to make changes or modifications within the scope of the concept of the invention disclosed in this specification, within the scope of the disclosure, and / or within the skill and knowledge of the art. The above-described embodiments illustrate the best mode for carrying out the technical idea of the present invention, and various modifications required for specific application fields and uses of the present invention are also possible. Accordingly, the detailed description of the invention is not intended to limit the invention to the disclosed embodiments. It is also to be understood that the appended claims are intended to cover such other embodiments.

10: Portable microorganism detection device 100: Housing member
110: outer container 130: inner container
200: support member 210: first support member
230: second support part 250: third support part
300: temperature regulating member 310: heating unit
330: temperature sensor unit 350: temperature controller
400: detecting member 410: light source part
430: light source control unit 450:
451: observation hole 453: observation cover

Claims (18)

A mobile-type microorganism detecting apparatus for detecting a microorganism by culturing,
A housing member including an outer container having a handle for allowing an operator to move and an inner container located inside the outer container and having a culture space for culturing the microorganisms therein;
A support member which is located inside the inner container and is divided into a plurality of spaces so that the microorganisms can be cultured in the culture space from samples collected by different methods;
A temperature regulating member for regulating the internal temperature so that the microorganisms are cultured in the internal space of the housing member; And
And an inspection member for inspecting whether or not a specific microorganism is included in the microorganisms cultured after a predetermined incubation time,
Wherein the support member comprises:
A first support on which the first sample is placed;
A second support portion that is adjacent to the first support portion, the second support portion being different from the first sample; And
And a third support portion that is adjacent to the first support portion and the second support portion, wherein a third sample different from the first sample and the second sample is placed,
A plurality of exhaust holes are formed on one side of the outer container so that the fluid inside and the outside air can flow,
The inspection member
A light source unit positioned inside the outer container;
A light source control unit for controlling whether or not the light source unit emits light; And
And an observing unit located in the outer container to observe the state of the light irradiated from the light source unit after passing through the cultured sample,
The observation section
An observation hole located at one side wall of the outer container and an observation cover coupled to one side wall of the outer container so as to cover or open the observation hole,
Wherein the light source unit is located on the outer side of the inner container and is provided on an inner wall of the outer container on which the observation hole is located and is provided with a UV lamp and the illuminance is 800 lux or more. delete The method according to claim 1,
Wherein the first support portion includes a first plate having a plurality of first holes,
The second support portion includes a second plate having a plurality of second holes,
Wherein the number and size of the first holes and the second holes are different from each other. The method of claim 3,
Wherein the first sample or the second sample is cultured while being accommodated in a hermetically sealed container and inserted into the first hole and the second hole, respectively,
Wherein the third sample is cultured in a state of being accommodated in a dry film and disposed in an inner space of the third support. The method according to claim 1,
The temperature control member
A heating unit for heating the inner container;
A temperature sensing unit installed inside the outer container for sensing a temperature; And
And a temperature controller for receiving the internal temperature information of the internal container sensed by the temperature sensor and controlling the internal temperature. 6. The method of claim 5,
Wherein the heating unit is provided as a silicone heater which surrounds the inner container. The method according to claim 1,
The temperature control member
A heat transfer fluid supply unit disposed at a lower portion of the outer container and supplying a heat transfer fluid as a fluid heated or cooled in the outer container;
A temperature sensing unit installed inside the outer container for sensing a temperature; And
And a temperature controller for receiving the internal temperature information of the internal container sensed by the temperature sensor and controlling the internal temperature. 8. The method of claim 7,
Wherein the heat transfer fluid supply unit includes a thermoelectric element to heat or cool the heat transfer fluid. 9. The method of claim 8,
Wherein the temperature regulating member further comprises a lower plate provided at a lower portion of the outer container and having a fluid hole through which the heat transfer fluid can pass. 10. The method of claim 9,
Wherein the temperature regulating member further comprises an air blowing unit formed at a lower portion of the lower plate and supplying the heat transfer fluid heated or cooled by the heat transfer fluid supply unit to the inside of the outer container. 11. The method of claim 10,
Wherein the temperature control unit controls the heat transfer fluid supply unit to heat or cool the heat transfer fluid to maintain the temperature of the heat transfer fluid at a predetermined temperature based on internal temperature information received from the temperature sensing unit. The method according to claim 5 or 7,
Wherein the temperature sensing unit is spaced apart from the inner wall of the outer container. delete delete delete delete The method according to claim 1,
Wherein the inner vessel is provided with a transparent material. delete

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