KR20090052756A - A device for manufacturing regeneration of a three dimensional tissue - Google Patents

Abstract

The present invention provides a three-dimensional tissue reproducing apparatus that can convey two spindles together, and in addition, the two spindle directions by the rotary table to produce three-dimensional tissue reproduction more quickly through the shortest path of each spindle. To this end, the three-dimensional tissue reproducing apparatus is a first main axis for transporting along the X, Y, Z axis to produce a cell culture support and a second main axis for spinning the nanofibers between the cell culture support The three-dimensional tissue reproducing apparatus is configured, the base frame is supported on the ground, the collection table is installed on the base frame to form a cell culture support on the surface, and two X-axis fixed to the upper portion of the base frame A guide rail, a single Y-axis guide rail disposed on each of the X-axis guide rails and being conveyed in the X-axis direction, and an upper portion of the Y-axis guide rail. A pair of brackets having a Z-axis guide rail formed thereon, and a first main shaft and a first coupled shaft coupled to the Z-axis guide rails formed on the respective brackets and being transferred to the Z-axis. It is characterized by consisting of two spindles.

Plotter, Cell Culture Support, Electrospinning, Nanofiber, Bone

Description

3D Tissue Reproduction Apparatus {A DEVICE FOR MANUFACTURING REGENERATION OF A THREE DIMENSIONAL TISSUE}

The present invention provides a three-dimensional tissue reproducing apparatus that can convey two spindles together, and in addition, the two spindle directions by the rotary table to produce three-dimensional tissue reproduction more quickly through the shortest path of each spindle. In more detail, the cell culture support and the nanofibers integrated between the cell culture support can be quickly implemented using a single device so as to quickly produce three-dimensional tissue reproduction such as bone of a human body. It relates to a three-dimensional tissue reproduction apparatus of the structure that can be.

In general, when organs or tissues in the human body are damaged, cells, drug supports, etc. are provided to effectively regenerate tissues. The tissue regeneration supports must have a physiological activity that is physically stable at an implant site and controls regenerative efficacy. In addition, after forming new tissue, it must be degraded in vivo, and the degradation product must not be toxic.

The scaffold for tissue regeneration is conventionally made of a cell culture scaffold of sponge type, matrix type nanofiber or gel type using a polymer having a constant strength and shape, and the cell culture scaffold has a specific depth or height. Plays an important role in creating a dimensional organization.

Techniques for implanting scaffolds that serve as a framework for tissue regeneration and for regenerating tissue in vivo using self-healing power are called regenerative medicine or tissue engineering.

An example of tissue engineering is a method of regenerating articular cartilage, and the articular cartilage regeneration method forms an artificial prosthesis with support of cartilage cells, and then implants the artificial prosthesis at the site of injury, thereby inducing cartilage cells at the site of injury joint. Is to be played.

 The prosthetic prosthesis is composed of a support formed in a three-dimensional shape by using chondrocytes as seeds.

As a method for forming a support having a three-dimensional shape, a rapid prototyping method and a stacked rapid prototyping method have been used.

The rapid build-up method is a process of dividing a sheet into a plurality of layers in order to obtain a molded object of a desired shape, and then laminating them sequentially to produce a desired shape, and a plurality of three-dimensional shapes modeled by a CAD system having a certain thickness. After dividing into sheets and changing them into slice data, a sheet-like sheet is formed on the basis of the sheet and stacked to form a sculpture.

According to such a three-dimensional tissue reproduction method according to the prior art, since the three-dimensional shape is constantly modeled and divided into a plurality of sheets having a predetermined thickness, and the plurality of divided sheets are sequentially stacked to form However, there has been a disadvantage in that it takes a long time to manufacture and laminate sheets.

In addition, since the apparatus for modeling the conventional three-dimensional tissue regeneration can only form a cell culture support, there is a need for an apparatus capable of integrating nanofibers together.

The present invention has been made in view of the above problems, in the three-dimensional tissue reproduction production apparatus for manufacturing a three-dimensional tissue reproduction is inserted into the human body used for tissue regeneration, the three-dimensional tissue reproduction production apparatus The present invention provides a three-dimensional tissue regeneration apparatus comprising a first spindle for transporting along the X, Y, and Z axes to generate a cell culture support, and a second spindle for spinning nanofibers between the cell culture supports.

At this time, the three-dimensional tissue reproducing apparatus, the base frame is supported on the ground, the collection table is installed on the base frame to form a cell culture support on the surface, and the two X-axis guide fixed to the upper portion of the base frame A rail, a single Y-axis guide rail disposed on each of the X-axis guide rail and conveyed in the X-axis direction, are installed to be mutually symmetrical upright to be conveyed to the upper portion of the Y-axis guide rail, to the opposite sides It is characterized in that it comprises a pair of brackets Z-shaped guide rails are formed, and the first and second spindles are coupled to the Z-axis guide rails formed on each bracket and transferred to the Z-axis.

According to the apparatus for producing three-dimensional tissue reproduction according to the present invention, two main axes are conveyed together, and the two main axes are changed by a rotation table to more quickly produce three-dimensional tissue reproduction through the shortest path of each main axis. There are features that can be produced.

In addition, since the cell culture support and the nanofibers accumulated between the cell culture support can be quickly implemented using a single device, the production time can be shortened, and the cell growth support can be formed by forming a nano-class cell culture support. There is an advantage that can be improved.

Hereinafter, a three-dimensional tissue reproduction apparatus according to the present invention will be described in detail together with the accompanying drawings.

1 is a block diagram of a three-dimensional tissue reproduction apparatus according to the present invention, Figure 2 is a side view of FIG.

As shown in Figures 1 and 2, the three-dimensional tissue reproducing apparatus of the present invention is conveyed together with the two main axes, and the two main axis direction is changed by the rotary table to more quickly through the shortest path of each main axis It relates to a three-dimensional tissue reproduction apparatus 10 of the structure capable of producing a three-dimensional tissue reproduction.

The three-dimensional tissue reproduction apparatus 10 is composed of six parts, which are the base frame 11, the collection table 17, the X-axis guide rail 12, and the Y-axis guide rail 13 ), A bracket 14, a first spindle 15 for producing a cell culture support, and a second spindle 16 for spinning nanofibers between the cell culture supports.

Here, the base frame 11 supports the ground and has a structure in which a collection table 17 is formed at the center portion.

Here, the collection table 17 is a site where the cell culture support is constructed on the surface, and the Peltier element 171 is mounted on the bottom thereof, so that the cell culture support formed on the surface and the nanofibers integrated between the cell culture support. It is configured to control the speed of hardening.

Here, the Peltier element 171 is equipped with a P-type semiconductor and a Peltier element in which an N-type semiconductor is mixed so that the cooling surface and the heating surface are changed so that the rate of solidification of the nanofibers integrated between the cell culture support and the cell culture support is increased. It can be configured for even faster adjustments.

The Peltier element 171 changes the flow of electrons and holes of the Peltier element according to the direction in which the voltage flows. As a result, the Peltier element 171 may selectively control the cooling and heating surfaces for emitting and absorbing heat.

In addition to the Peltier element, a general heater may be used instead.

On the other hand, the collection table 17 is coupled to the auxiliary guide rail 111 formed in the base frame 11 so as to be transported to set the shortest distance between the first spindle 15 and the second spindle 16 more quickly To prepare a three-dimensional tissue reproduction can be prepared. In addition, the X-axis guide rail 12 is fixed to both sides of the base frame 11 to provide a structure in which the first and second spindles 15 and 16 can be moved to the X-axis.

And the Y-axis guide rail 13 is a structure that is disposed in the Y-axis direction on the upper portion of each of the X-axis guide rails (12).

The upper portion of the Y-axis guide rail 13 is equipped with a rotary table 18, and the upper portion of the rotary table 18, the first bracket 15 and the second spindle 16 is coupled to each of the two brackets ( 14) are installed and configured to face each other.

Here, each of the brackets 14 has a Z-axis guide rail 141 as a surface facing each other, and each of the Z-axis guide rails 141 has a first main shaft 15 and a second main shaft 16. Coupled to each major axis (15, 16) provides a structure that can be transferred to the Z axis.

The structure as described above conveys two main shafts (15, 16) to the X, Y axis through a single Y-axis guide rail 13 disposed on the X-axis guide rail 12, the Y-axis guide The position of the two main shafts 15 and 16 is changed by the rotary table 18 disposed on the upper side of the rail 13. Provide a structure to help.

On the other hand, the first spindle (15) is configured to be equipped with a plotter 151 to form a cell culture support that is a basic skeleton of a three-dimensional shape to the upper portion of the collection table (17).

The plotter 151 is composed of a solution storage tank 151b in which a biopolymer (polymer) solution is stored, and a plotter nozzle 151a, and configured to send a cell culture support.

The second spindle 16 has an electrospinning machine that radiates nanofibers in the form of fiber yarn between and on the surface of the three-dimensional cell culture support formed by the plotter 151 above the collection table 17 ( 161 is mounted and configured.

Here, the electrospinner 161 may include a solution storage tank 161b in which a biopolymer solution is stored, an electrospinning nozzle 161a for injecting a solution supplied from the solution storage tank 161b, and the electrospinning nozzle 161a. ) And a voltage generator 161c connected to the nozzle and the collection table 17 such that the solution radiated from the nozzle by applying voltage to the collection table 17 is finely radiated in the form of nanofibers by electrospinning. .

Hereinafter, the operation of the 3D tissue reproducing apparatus will be briefly described.

Figure 4a. Figure 4b is a photograph of the cell culture support built on top of the collection table, Figure 5 is a photograph showing a nanofiber integrated between the cell culture support in Figures 4a and 4b.

Prior to describing the present invention, a process of photographing a patient's bone, converting it into 3D coordinate data, receiving the control, and controlling the 2D tissue reproducing apparatus 10 through the control unit should be preceded.

First, the first main axis 15 and the second main axis are moved to X, Y, and Z along the X-axis guide rail 12 and the Y-axis guide rail 13 according to the 3D coordinate data, so that the upper end of the collection table 17 Located in

The first spindle 15 then descends on the Z axis such that the plotter nozzle 151a is close to the upper surface of the collection table 17.

Thereafter, pressure is applied to the inside of the solution storage tank 151b so that the polymer solution is discharged from the plotter nozzle 151a.

Here, the polymer solution is sent out and the first spindle 15 moves along the X, Y, and Z axes to build a skeleton by stacking the polymer solution to form a cell culture support as shown in FIGS. 4A and 4B.

Thereafter, the first spindle 15 is transferred to the upper portion of the cell culture support along the Z-axis guide rail 141 of the bracket 14, and the rotary table 18 is rotated 180 degrees so that the second spindle 16 is the cell. The position is changed to be disposed above the culture support.

Then, the second spindle 16 is rapidly lowered to the cell culture support and between the three-dimensional cell culture support formed by the plotter 151 through the electrospinner 161 and on the surface thereof, as shown in FIG. Yarn-shaped nanofibers are spun to integrate the nanofibers into the cell culture support.

And repeating the above process to build a three-dimensional tissue reproduction that can replace the bone shape of the human body.

Finally, the cells extracted from the human body are transplanted to the constructed three-dimensional tissue regenerated and completed by incubating the three-dimensional tissue regenerated.

Although the present invention has been described with reference to the accompanying drawings, it will be apparent to those skilled in the art that many different and obvious modifications are possible without departing from the scope of the invention from this description. Therefore, the scope of the invention should be construed by the claims described to include many such variations.

1 is a block diagram of a three-dimensional tissue reproduction apparatus according to the present invention,

2 is a side view of FIG. 1;

3 is an operation of FIG.

Figure 4a. Figure 4b is a photograph of the cell culture support built on top of the collection table

Figure 5 is a photograph showing the nanofibers integrated between the cell culture support in Figures 4a and 4b

<Description of the symbols for the main parts of the drawings>

10: three-dimensional tissue reproduction apparatus

11: base frame 111: auxiliary guide rail

12: X axis guide rail

13: Y-axis guide rail 14: Bracket

141: Z-axis guide rail

15: first spindle 151: plotter

151a: plotter nozzle 151b: solution storage tank

16: 2nd spindle 161: electrospinning machine

161a: electrospinner nozzle 161b: solution storage tank

161c: voltage generator

17: collection table 171: Peltier element

18: rotating table

Claims (8)

In the three-dimensional tissue reproduction apparatus for producing a three-dimensional tissue reproduction is inserted into the human body used for tissue regeneration, The three-dimensional tissue reproducing apparatus 10 is a first spindle 15 for transporting along the X, Y, Z axis to produce a cell culture support, and a second spindle for spinning nanofibers between the cell culture support Consisting of 16, The three-dimensional tissue reproduction apparatus 10, A base frame 11 supported on the ground, a collection table 17 mounted on the base frame 11 to form a cell culture support on the surface thereof, and two X axes fixed to an upper portion of the base frame 11. A guide rail 12, a single Y-axis guide rail 13 disposed on each of the X-axis guide rails 12 and being conveyed in the X-axis direction, and being conveyed together on the upper portion of the Y-axis guide rail 13; It is installed to be symmetrically upright mutually, the pair of brackets (14) formed with the Z-axis guide rails (141) as opposed to each other, and is coupled to the Z-axis guide rails (141) formed on each bracket (14) 3D tissue reproducing apparatus, characterized in that consisting of the first spindle (15) and the second spindle (16) to be transferred to the Z axis. The method of claim 1, The first spindle 15 and the second spindle 16 are coupled to the Y-axis guide rail 13 to the bottom surface of the bracket 14 so as to be mutually changeable rotation table 18 for conveying to the Y-axis Apparatus for producing a three-dimensional tissue reproduction, characterized in that the mounting. The method of claim 1, The first spindle (15) is a three-dimensional tissue reproduction apparatus, characterized in that the plotter (151) for forming a cell culture support that is a basic skeleton of the three-dimensional shape is mounted to the upper portion of the collection table (17). The method of claim 1, An electrospinner 161 radiating nanofibers in the form of fiber yarn between the three-dimensional cell culture supports formed by the plotter 151 on the second spindle 16 and on the surface thereof. 3D tissue reproducing apparatus, characterized in that the mounting. The method of claim 4, wherein The electrospinner 161 is; A solution storage tank (161b) in which the biopolymer solution is stored; An electrospinning nozzle 161a for injecting a solution supplied from the solution storage tank 161b, A voltage is applied to the nozzle 161b and the collection table 17 and connected to the nozzle 161b and the collection table 17 such that the solution radiated from the nozzle 161b is finely spun in the form of nanofibers by electrospinning. 3D tissue reproducing apparatus characterized in that it comprises a voltage generator (161c). The method of claim 4, wherein The plotter (151) is a three-dimensional tissue reproduction apparatus, characterized in that consisting of a solution storage tank (151b) and a plotter nozzle (151a) in which the biopolymer solution is stored. The method of claim 1, The collection table (17) is a three-dimensional tissue reproduction apparatus characterized in that the transfer is coupled to the auxiliary guide rail (111) formed in the base frame (11). The method according to claim 1 or 7, 3, characterized in that the Peltier element 171 is mounted on the bottom of the collection table 17 to control the rate of solidification of the nanofibers integrated between the cell culture support and the cell culture support that are constructed and deposited on the surface. Dimensional tissue reproduction apparatus.

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