CN106031800A - Hollow stent for inducing regeneration and moulding of cartilage/bone tissues - Google Patents
Hollow stent for inducing regeneration and moulding of cartilage/bone tissues Download PDFInfo
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- CN106031800A CN106031800A CN201510099776.8A CN201510099776A CN106031800A CN 106031800 A CN106031800 A CN 106031800A CN 201510099776 A CN201510099776 A CN 201510099776A CN 106031800 A CN106031800 A CN 106031800A
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Abstract
The invention relates to a hollow stent for inducing the regeneration and moulding of cartilage/bone tissues. The structure of the stent comprises an overall three dimensional hollow structure; and a plurality of holes in the surface. The hardness should meet the requirements of cartilage/bone tissues in the repaired parts. The stent should have a good biocompatibility and is made of a biologically degradable material. The biologically degradable material can be made into filling and repairing materials such as artificial auricular cartilage stents, artificial nose bridges, and the like, and can also be made into artificial bone stents with various sizes. The prepared stents with special structures can be combined with cultured cartilage/bone cells in vitro and then implanted into human body. Or, small cartilage blocks and cartilage sheets are filled into the hollow stents. The stents can be implanted into human body at first, and then cartilage/bone cells are injected into the stents. The inoculated cartilage/bone cells proliferate and pass down in the hollow three-dimensional stents, bones or cartilage tissues with the shape of the stents gradually form during the period, and tissues with similar functions and shapes can be obtained.
Description
Technical field
The present invention relates to cartilage/bone tissue engineering technique field, be specifically related to a kind of hollow stent inducing cartilage/bone tissue regeneration molding.
Background technology
The appearance of organizational project in recent years, reparation for tissue defect provides a new approach, bone/cartilage tissue engineering is studied more in field of tissue engineering technology, the research emphasis of bone/cartilage tissue engineering is concentrated mainly on timbering material at present, the raw material of such as support, biocompatibility, space structure, mechanical performance etc..Bone/cartilage frame clinical practice is common in plastic surgery and orthopaedics, but the increase along with application, there is also many weak points, such as plastic surgery, the assembled artificial ear cartilage frame Medpor that uses clinically repairs microtia, although solve from the autologous additional injuries cutting costicartilage with painful and carve the complicated processes of autologous costicartilage, but this artificial cartilage uses polythene material to make, hardness is big and elasticity is not enough, is easily caused frameworks exposure etc..Preferably support is key one ring that organizational project successfully builds.In bone/cartilage tissue engineering, preferable support be not only the propagation of cell, substrate offer steric environment is provided, and the biocompatibility that (1) is good should be had the property that；(2) good biological degradability；(3) effective surface activity；(4) certain plasticity；(5) there is three-dimensional porous structure.
Summary of the invention
It is an object of the invention to provide a kind of hollow stent inducing cartilage/bone tissue regeneration molding, after inoculation cartilage or osteoblast, cell is bred in hollow three-dimensional rack, is passed on, or clog in tiny cartilage/spongiosa bone piece, cartilage thin slice to hollow stent, cartilage/bone tissue gradually merges, gradually form autologous bone or the cartilaginous tissue with contoured cradle, obtain and original profile and intimate organizational structure to the full extent.
In order to reach this purpose, the technical solution adopted in the present invention is:
A kind of hollow stent inducing cartilage/bone tissue regeneration molding, the architectural feature of support includes 1, overall in 3 D stereo hollow structure；2, can there be multiple hole on surface；3, support hardness meets repairing position cartilage/bone organizational requirements；4, there is good biocompatibility；5, timbering material can be Biodegradable material；6, support can be fabricated to the filling and repairing things such as artificial ear cartilage frame, the bridge of the nose and chin, it is possible to is fabricated to the artificial bone scaffold differed in size.
Described 3 D stereo hollow structure, can be monolayer or multilayer hollow structure, it is possible to for multiple tiny hollow structures, being connected or be separated by between each hollow structure, bone or chondrocyte are bred in hollow three-dimensional rack, passed on.
Described rack surface can have multiple hole, including arbitrary size and any geometryFigureShape hole, it is possible to without hole.
Described support consistency and elasticity, according to the hardness requirement of repairing position cartilage/bone tissue, meets requirement from cartilage to bone structure.
Described support has good biocompatibility, and cell adhesion forces is good, acellular toxic and side effects.
Described timbering material includes Biodegradable material, also includes non-degradable material, even includes the material that can merge completely with cartilage or bone, and selected timbering material all can induce cartilage/bone cell or tissue to regenerate, such as PLGA, chitosan, polyurethane, polyethylene.
Described support can be fabricated to the filling and repairing things such as artificial ear cartilage frame, the bridge of the nose and chin, it is possible to is fabricated to the artificial bone scaffold differed in size.
Accompanying drawing explanation
Embodiment
The preparation of artificial ear cartilage frame and application
1, auricle is obtained three-dimensionalFigurePicture: use multi-slice Spiral CT to obtain the synusia with reference to ear contoursFigurePicture, extracts the cartilage profile with reference to every layer of auricle, by the cartilage profile synusia of gainedFigureObtaining Ear cartilage threedimensional model as after reconstruct, reverse process uses the Mimics software of Materialise to obtain Ipsilateral threedimensional modelFigurePicture, and utilize Mimics software, according to Ipsilateral calvarial bone situation, Ear cartilage shape carries out the correction of necessity, modifies design by the supporting structure feature of the present invention to Ear cartilage threedimensional model.
2, the preparation of artificial ear cartilage frame: utilize biological 3D printing technique or traditional manufacturing technique to obtain the artificial ear cartilage frame of the present invention, the support key structural feature produced is 3 D stereo hollow structure, and porous surface hole, pore diameter range is that (large aperture is more beneficial for a large amount of chondrocyte and can grow into 200 ~ 1000 μm, and nutrient substance, the exchange of metabolic waste and the formation of vascularization, it is more beneficial for the adhesion of flap before and after ear), the material that support is used includes Biodegradable material, also non-degradable material is included, even include the material that can merge completely with cartilage, selected timbering material all can Induction of chondrocytes or tissue regeneration, such as PLGA, chitosan, polyurethane, polyethylene.
3, the operation instruction of artificial ear cartilage frame: use the present invention the artificial ear cartilage frame made by support can in vitro first with the chondrocyte cultivated or stem cell (the residual Ear cartilage cell after amplification, the cell suspension that mesenchymal stem cells MSCs after induction or chondrocyte and stem cell co-culture) combine after, implant again, or directly clog tiny cartilage block, cartilage thin slice is in hollow stent, support can also first implant, divide multiple spot through being injected in support hollow structure the chondrocyte after amplification again, the cell of inoculation is bred in hollow three-dimensional rack, pass on, gradually form the autologous cartilaginous tissue with contoured cradle, obtain and the intimate organizational structure of original profile to the full extent.
Claims (7)
1. the hollow stent inducing cartilage/bone tissue regeneration molding, it is characterised in that: the structure of support includes 1) overall in 3 D stereo hollow structure；2) can there be multiple hole on surface；3) support hardness meets repairing position cartilage/bone organizational requirements；4) there is good biocompatibility；5) timbering material can be Biodegradable material；6) support can be fabricated to the filling and repairing things such as artificial ear cartilage frame, the bridge of the nose and chin, it is possible to is fabricated to the artificial bone scaffold differed in size.
A kind of hollow stent inducing cartilage/bone tissue regeneration molding the most according to claim 1, it is characterized in that: whole supporting structure can be 3 D stereo hollow structure, can be monolayer or multilayer hollow structure, it is alternatively multiple tiny hollow structures, being connected between each hollow structure or be separated by, bone or chondrocyte are bred in hollow three-dimensional rack, are passed on.
A kind of hollow stent inducing cartilage/bone tissue regeneration molding the most according to claim 1, it is characterised in that: rack surface can have multiple hole, including arbitrary size and any geometryFigureShape hole, it is possible to without hole.
A kind of hollow stent inducing cartilage/bone tissue regeneration molding the most according to claim 1, it is characterised in that: support consistency and elasticity, according to the hardness requirement of repairing position cartilage/bone tissue, meets requirement from cartilage to bone structure.
A kind of hollow stent inducing cartilage/bone tissue regeneration molding the most according to claim 1, it is characterised in that: support has good biocompatibility, and cell adhesion forces is good, acellular toxic and side effects.
A kind of hollow stent inducing cartilage/bone tissue regeneration molding the most according to claim 1, it is characterized in that: timbering material includes Biodegradable material, also non-degradable material is included, even include the material that can merge completely with cartilage or bone, selected timbering material all can induce cartilage/bone cell or tissue to regenerate, such as PLGA, chitosan, polyurethane, polyethylene.
A kind of hollow stent inducing cartilage/bone tissue regeneration molding the most according to claim 1, it is characterised in that: use thisInventionSupport can be fabricated to the filling and repairing things such as artificial ear cartilage frame, the bridge of the nose and chin, it is possible to be fabricated to the artificial bone scaffold differed in size.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510099776.8A CN106031800A (en) | 2015-03-08 | 2015-03-08 | Hollow stent for inducing regeneration and moulding of cartilage/bone tissues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510099776.8A CN106031800A (en) | 2015-03-08 | 2015-03-08 | Hollow stent for inducing regeneration and moulding of cartilage/bone tissues |
Publications (1)
| Publication Number | Publication Date |
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| CN106031800A true CN106031800A (en) | 2016-10-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510099776.8A Pending CN106031800A (en) | 2015-03-08 | 2015-03-08 | Hollow stent for inducing regeneration and moulding of cartilage/bone tissues |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106421893A (en) * | 2016-12-08 | 2017-02-22 | 大连裕辰科技发展有限公司 | Allogeneic decalcified bone and titanium stent combined material for rhinoplasty filling and preparation method of allogeneic decalcified bone and titanium stent combined material |
| CN106421890A (en) * | 2016-12-08 | 2017-02-22 | 大连裕辰科技发展有限公司 | Material used for autologous cortical bone synostosis silica gel particle for nasal plasty and preparation method of material |
| CN106473838A (en) * | 2016-12-08 | 2017-03-08 | 大连裕辰科技发展有限公司 | A kind of material filling autologous synostosis titanium framework for nose shaping and preparation method thereof |
| CN106474552A (en) * | 2016-12-08 | 2017-03-08 | 大连裕辰科技发展有限公司 | A kind of material filling allogeneic decalcification synostosis silica gel particle for nose shaping and preparation method thereof |
| CN106620838A (en) * | 2016-12-08 | 2017-05-10 | 大连裕辰科技发展有限公司 | Material for filling artificially synthesized bone matrix combined titanium bracket for nose shaping and preparation method thereof |
| CN110772356A (en) * | 2019-11-15 | 2020-02-11 | 朱伟伟 | Implanted auricular cartilage composite support |
| CN112354017A (en) * | 2020-11-19 | 2021-02-12 | 中国医学科学院生物医学工程研究所 | 3D printed mechanical bionic auricular cartilage tissue engineering scaffold and manufacturing method thereof |
| CN113749825A (en) * | 2020-07-14 | 2021-12-07 | 浙江大学 | Frame type bone joint prosthesis and preparation method and application thereof |
| WO2022038235A1 (en) * | 2020-08-19 | 2022-02-24 | Dirk Hellekes | Method for providing an implant, implant, and use of the implant for reconstruction of a body part |
-
2015
- 2015-03-08 CN CN201510099776.8A patent/CN106031800A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106421893A (en) * | 2016-12-08 | 2017-02-22 | 大连裕辰科技发展有限公司 | Allogeneic decalcified bone and titanium stent combined material for rhinoplasty filling and preparation method of allogeneic decalcified bone and titanium stent combined material |
| CN106421890A (en) * | 2016-12-08 | 2017-02-22 | 大连裕辰科技发展有限公司 | Material used for autologous cortical bone synostosis silica gel particle for nasal plasty and preparation method of material |
| CN106473838A (en) * | 2016-12-08 | 2017-03-08 | 大连裕辰科技发展有限公司 | A kind of material filling autologous synostosis titanium framework for nose shaping and preparation method thereof |
| CN106474552A (en) * | 2016-12-08 | 2017-03-08 | 大连裕辰科技发展有限公司 | A kind of material filling allogeneic decalcification synostosis silica gel particle for nose shaping and preparation method thereof |
| CN106620838A (en) * | 2016-12-08 | 2017-05-10 | 大连裕辰科技发展有限公司 | Material for filling artificially synthesized bone matrix combined titanium bracket for nose shaping and preparation method thereof |
| CN110772356A (en) * | 2019-11-15 | 2020-02-11 | 朱伟伟 | Implanted auricular cartilage composite support |
| CN110772356B (en) * | 2019-11-15 | 2021-10-12 | 朱伟伟 | Implanted auricular cartilage composite support |
| CN113749825A (en) * | 2020-07-14 | 2021-12-07 | 浙江大学 | Frame type bone joint prosthesis and preparation method and application thereof |
| CN113749825B (en) * | 2020-07-14 | 2022-08-05 | 浙江大学 | Frame type bone joint prosthesis and preparation method and application thereof |
| WO2022038235A1 (en) * | 2020-08-19 | 2022-02-24 | Dirk Hellekes | Method for providing an implant, implant, and use of the implant for reconstruction of a body part |
| CN112354017A (en) * | 2020-11-19 | 2021-02-12 | 中国医学科学院生物医学工程研究所 | 3D printed mechanical bionic auricular cartilage tissue engineering scaffold and manufacturing method thereof |
| CN112354017B (en) * | 2020-11-19 | 2022-07-26 | 中国医学科学院生物医学工程研究所 | 3D printed mechanical bionic auricular cartilage tissue engineering scaffold and manufacturing method thereof |
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Application publication date: 20161019 |