KR20090014693A - Skin care sheet using nano scale nonwoven and process therefore - Google Patents

Abstract

A skin care sheet by using nano scale nonwoven fabric is provided to improve biocompatibility, use feeling and adhesion of the sheet, and supply the active ingredients to the skin by adding natural materials having excellent effects in the skin care and skin health. The skin care sheet by using nano scale nonwoven fabric is manufactured by electrically spraying the nature composite on the mask sheet, wherein the nature composite is prepared by mixing 5-15 wt.% of fiber-forming natural polymer, 0.1-30 wt.% of functional additive, 0.1-10 wt.% of cross-linking agent, and 45-94.8 wt.% of distilled water, and stirring the mixture at 0-70 deg. C for 1-48 hours.

Description

Skin care sheet using nanofiber nonwoven fabric and manufacturing method thereof {Skin care sheet using nano scale nonwoven and process therefore}

The present invention relates to a skin care pack using nanofibers and a method of manufacturing the same. More specifically, a non-woven sheet to be used for skin care, namely cotton, rayon, modal, lyocell, polyester (PET), nylon (Nylon), and polypropylene. Nanofiber web by electrospinning composites of polyvinyl alcohol (PVA), chitosan, starch, elastin, alginate, proteoglucan, fluorane, functional additives and crosslinking agents, which are fiber-forming natural polymers (PP) or blended products It is to provide a skin care pack that can form and excellent skin adhesion and skin adhesion, and can give an effective ingredient dramatically.

Since the introduction of the electrospinning method, many polymer materials have been manufactured into ultrafine nanofibers through the electrospinning method. Since there are many differences in mechanisms and systems from existing solutions and melt spinning methods, research on materials such as polymers and inorganic materials having low or no fiber formation ability as well as other synthetic fibers and polymers for natural fibers is being conducted. In addition, much attention has been focused on the preparation of nano-island oil through electrospinning of biodegradable and biocompatible polymers. These nanofibers can be used for various applications such as life science, tissue engineering, filter media, protective clothing, and sensors (nano-sensors). Application of the filter media is the subject of recent research. In particular, research on medical materials such as nanofibers and biopolymers for biotechnology and tissue engineering is being actively conducted. More than 80% of the major themes of nanofiber papers published in 2003 are focused on biomedical science on scaffolds. Mostly, studies on the electrospinability of biocompatible and biodegradable polymers such as PLA, PGA, PLGA, Collagen, and elastin, the possibility of cell culture on these ultrafine nanofiber webs, and their application to DDS using biodegradation rate control have been reported. The reason why the electrospun nanofibers are applicable to biomedical applications is that they have structural properties similar to those of the extracellular matrix and physically similar properties (YS Nam, Polym . Sci . Technol., 14 (3)). , 274 (2003). Indeed, several studies have shown that nanofibers are evaluated as materials that promote cell adhesion and growth from body tissues to the porous surface of the web. If the drug delivery time of the DDS can be controlled, it can maintain the effect of the drug and increase the duration of treatment, thereby increasing the therapeutic effect and preventing the drug from being released in large quantities at once. Kenawy et al. (ER Kenawy et al. Biomaterials , 24 , 907 (2003)) post-study studies on electrospinning poly (ethylene-co-vinyl acetate) (EVA), which is not biodegradable but is biocompatible. As a result of the study on drug release and cell culture, EVOH nanofibers can be used to make high fibrous high solid materials and to be applied to various biomedical applications such as cell culture and tissue culture, wound treatment, and drug delivery system. In the study of drug delivery system (DDS), Zeng (J. Aeng et al. J. Control . Release , 92 , 227 (2003)) is a biodegradable raw material poly (L-lactic acid) (PLA). After the nanofibers were prepared by injecting and electrospinning the drug into the nanofibers, the release properties of the drug were evaluated. Receive. The release of drug depends on the presence of proteinase K, which degrades PLA. As such, research and industrialization are progressing in the medical industry.

An object of the present invention can provide a skin-friendly, bio-friendly material by grafting to the cosmetic industry by taking full advantage of the nano-nonwoven fabric as described above, it can provide excellent efficacy, such as feeling and adhesion that gently adheres to the skin. . In addition, it is to provide a skin care pack that can significantly give an active ingredient by adding a natural material with excellent efficacy in skin care and skin health.

The present invention relates to a skin care pack using nanofibers and a method of manufacturing the same. More specifically, a non-woven sheet to be used for skin care, namely cotton, rayon, modal, lyocell, polyester (PET), nylon (Nylon), and polypropylene. Polyvinyl alcohol (PVA), chitosan, collagen, elastin, alginate, proteoglucan, starch, pullulan, PLA, PGA, PLGA or blended materials and functional additives Electrospinning a composite of a crosslinking agent is formed to form a nanofiber web to provide a skin care pack excellent in skin adhesion and skin adhesion, and can significantly give effective ingredients.

Fiber-forming natural polymer 5% to 15% by weight , 0.1% to 30% by weight of functional additives, 0.1 to 10% by weight of crosslinking agent, 45% to 94.8% by weight of distilled water at 0-70 ° C for 1 hour to 48 hours A step of preparing a natural composite prepared by stirring and dissolving;

Electrospinning manufacturing step at 0 ~ 40 kV on normal mask sheet;

Nanofiber nonwoven fabric skin care sheet manufacturing method comprising

As a material capable of maintaining a shape among the components of the skin care sheet, cotton, rayon, modal, lyocell, polyester (PET), nylon (Nylon), polypropylene (PP) or two or more blend sheets, The thickness is 30g / m2 to 120g / m2 in consideration of the feeling of use when the skin is used, preferably characterized in that 45g / m2 to 80g / m2. In addition, in order to prepare a nano nonwoven fabric using a polyvinyl alcohol (PVA), chitosan, collagen, elastin, alginate, proteoglucan, starch, pullulan, PLA, PGA, PLGA or a blend of these materials It can increase the skin fit and feel, and the polymer should contain 5% to 15% by weight, and if the polymer is less than 5% by weight, fibers may not be formed, resulting in uneven products, and skin irritation and skin allergy. It may also affect. On the other hand, in the case of more than 15% by weight, the extrusion force is increased, the electrospinning is not smooth. In case of these non-woven fabrics, MTT assay was performed on the natural composites to confirm skin affinity (Fig. 2).

In addition, skin-friendly natural materials are used to give the skin's efficacy, and natural materials include Eoseongcho, Doin, Doinpi, Panramus, Rigida, Situation, Ganoderma lucidum, Sin Ehwa, Pogongyoung, Yulpi, Camellia, Phytoncide, Bud Tree, birch, pine extract, potato, licorice, red ginseng, brown alga, guar gum, oyster, grape seed, honey, green tea, carrot, sugar, camellia oil, wild chrysanthemum, strawberry, rosemary, locust bean gum, macadamia nut oil, Matricaria, Starch, Bifidus, Saffron Oil, Cereal Peel, Sage, Scrubber, Vegetable Squalane, Silk, Acacia, Rice, Mugwort, Almond Oil, Avocado Oil, Ivy, Aloe, Corn, Olive Oil, Milk Protein, Turmeric, At least one or two from oil-soluble licorice, ginseng, peony, rose, iris, horoscope, gardenia, coconut oil, parsley, henna, hosttail, jojoba oil, ocher and the like were used. Antioxidant, antimicrobial, anti-inflammatory, anticytotoxic, anti-irritant, anti-allergic, moisturizing, whitening, astringent, soothing effects, etc. can be given, having a content between 0.1% to 30% by weight It features. It is ineffective when less than 0.1% by weight, and in the case of more than 30% by weight has the disadvantage that the unique pigment of the extract can affect the whole product.

In addition, the cross-linking agent may be mixed for proper adhesion of the nonwoven fabric sheet and the nanowoven fabric to complete the product, characterized in that it has a content of 0.1% to 10% by weight. As the crosslinking agent, at least one or two or more from epichlorohydrin, boric acid, acrylic acid, polyvinyl alcohol resin, chitosan, and the like may be used.

The nanofiber nonwoven skin care sheet of the present invention can impart skin affinity, biocompatibility, and excellent feeling, and can effectively and dramatically impart an active ingredient to the skin as applied to the skin, in addition to the purpose of skin care, wounds, It is an agent that promotes healing of wounds by giving effective enemies to acne, atopy and diet, to reduce the pain caused by wounds and to prevent severe scars by returning as close as possible to the state before the injury. It can give an excellent efficacy to. Wrinkle treatment dressing that releases active ingredients slowly upon contact with skin to promote beauty and wound healing, regenerate damaged skin, and regenerate skin as soon as possible during wound healing. And it can be usefully used as a skin care sheet. In particular, the nanofiber non-woven skin care sheet can be effectively used in skin care, wound treatment, atopy, oral wound treatment, diet patch, exfoliation, acne treatment, scar removal, bands.

Hereinafter, embodiments of the present invention will be described in more detail a part of the present invention, but the content of the present invention is not limited thereto.

Example 1

Nanofiber nonwoven solution manufacturing

After adding 3.5 g of water-soluble chitosan and 3.5 g of PVA to 89 g of purified water, the mixture was heated up to 70 degrees, dissolved, and then 1 g of situation mushroom extract and 3 g of epichlorohydrin were added to prepare a natural composite solution for manufacturing a nonwoven fabric.

Skin Beauty Sheet Manufacturing

After the natural composite was prepared, the skin composite sheet was prepared by electrospinning (electrospinning conditions) the natural composite on a nonwoven fabric of 60 g / m ² mask form.

Examples 2-5

EXAMPLES Ingredients Example 2 Example 3 Example 4 Example 5 Purified water to. 100 to. 100 to. 100 to. 100 Chitosan - 3.5 3.5 - PVA 7 - 3.5 3.5 Collagen - 3.5 - 3.5 Starch - - One One Situation Mushroom Extract One - One - Green Tea Extract - One - One Epichlorohydrin 3 5 Boric acid        3 5 100% cotton (60 g / m2) ? ? 100% cotton (40 g / m2) ? Cotton / rayon (8: 2) (60g / m2) ?

Example 6 MTT assay

    The effect of the sample on macrophage Raw 264.7 cells was evaluated by MTT assay of Carmichael et al. After dispensing 5 × 104 cells / well in a 96well plate, it was replaced with serum free EMEM when reaching 80% confluency. Natural complexes were added for each concentration, and after 4 hours of treatment with MTT (5mg / mL) in viable cells after culture, the medium was removed, and the resulting formazan crystal was dissolved in DMSO and absorbed at 540 nm. As a result of the measurement in the present invention, the cytotoxicity of a single substance and a mixed substance such as Chitosan and PVA was not shown.

Example 7 Cell Activity Test

  The cell activity test of macrophage Raw 264.7 cells was performed on the sheet prepared in Example 1. After dispensing the cells in T-bottle, the cells were replaced with serum free EMEM when 80% confluency was reached. The prepared sheet was placed in a 6 well plate, and macrophage Raw 264.7 cells were dispensed with 5 × 104 cells / well of cells, and cell activities were measured for 24 hours and 48 hours. As a result of the measurement by the above method, the cell culture was increased by 2-4 times after 48 hours, and the cell activity was excellent.

Example 8 Patch Test

  The patch test of the mask sheet using the nanofiber nonwoven fabric prepared in Example 1 was carried out. The patch test, which is a skin safety test, was determined based on the criteria of the International Contact Dermatitis Research Group as shown in Table 3. It was attached to the inner part of the arm and the skin reaction after 48 hours was confirmed.

  As a result of patch test on the applicant's skin, it was found that the dermatological problems such as redness, erythema and itching did not occur among the 20 applicants.

Table 3. Interpretation criteria of patch test suggested by International Contact Dermatitis Research Group

Recording Interpretation ?  Doubtful reaction: faint macular erythema only +  Weak (non-vesicular) positive reaction: erythema, infiltration, possibly papules ++  Strong (vesicular) positive reaction: erythema, infiltration, papules, vesicles +++  Extreme positive reaction: bullous reaction -  Negative reaction

Example 9 Investigation of the Fit of the Skin Beauticist

Examining the fit of each fabric and the general non-woven fabric sheet prepared in Examples 1 to 5 are shown in Table 3 below the results.

The fit was randomly extracted from 20 men and women aged 20 to 50 years, with a 9-point rating (9 = best; 8 = greatly good; 7 = normally good; 6 = slightly good; 5 = good or hateful; 4 According to = slightly disliked; 3 = usually disliked; 2 = very disliked; 1 = disliked most), the preference was evaluated three times, and the average of the final responded scores was summed.

division Fit Example 1 8.5 Example 2 8.2 Example 3 8.3 Example 4 8.4 Example 5 8.7 100% cotton 6.2 100% cotton (40 g / m2) 5.9 Cotton / rayon (8: 2) (60g / m2) 5.5

1 shows a schematic diagram of skin care mask pack.

Figure 2 shows the results of measuring the MTT assay of the natural complex.

Figure 3 shows the results of testing the cell activity of the nanofiber nonwoven fabric.

Claims (6)

Fiber-forming natural polymer 5% to 15% by weight , 0.1% to 30% by weight of functional additives, 0.1 to 10% by weight of crosslinking agent, 45% to 94.8% by weight of distilled water at 0-70 ° C for 1 hour to 48 hours Nanofiber non-woven skin care sheet prepared by electrospinning a natural composite prepared by stirring and dissolving onto a mask sheet In claim 1, the fiber-forming natural polymer is polyvinyl alcohol (PVA), chitosan, collagen, elastin, starch, pullulan, PLA, PGA, PLGA or a nanofiber nonwoven skin care sheet, characterized in a blend of these materials The functional additives in paragraph 1, Eoseongcho, Doin, Doinpi, Panrameun, Rigida, Sichuan, Ganoderma lucidum, Sinyihwa, Pogongyoung, Yulpi, Camellia, Phytoncide, Willow, Birch, Pine Extract, Potato, Licorice, Gosam, Brown algae , Guar gum, oyster, grape seed, honey, green tea, carrot, sugar, camellia oil, wild chrysanthemum, strawberry, rosemary, locust bean gum, macadamia nut oil, matricaria, starch, bipidus, saffron oil, lettuce skin, sage , Scrubber, vegetable squalene, silk, acacia, rice, mugwort, almond oil, avocado oil, ivy, aloe, corn, olive oil, milk protein, turmeric, oil-soluble licorice, ginseng, peony, rose, iris, celery, gardenia, coconut Nanofiber non-woven skin care sheet characterized by at least one or more from oil, parsley, henna, hostale, jojoba oil, ocher, etc. In claim 1, the crosslinking agent is a nanofiber nonwoven skin care sheet, characterized in that at least one or more from epichlorohydrin, boric acid, acrylic acid, polyvinyl alcohol resin, chitosan, etc. The mask sheet of claim 1, characterized in that one or more blend sheets from cotton, rayon, modal, lyocell, polyester (PET), nylon (Nylon), polypropylene (PP) and the like, the thickness is 30g / m2 to 120g Nanofiber nonwoven skin care sheet, characterized in that / m2 Fiber-forming natural polymer 5% to 15% by weight , 0.1% to 30% by weight of functional additives, 0.1 to 10% by weight of crosslinking agent, 45% to 94.8% by weight of distilled water at 0-70 ° C for 1 hour to 48 hours A step of preparing a natural composite prepared by stirring and dissolving; Electrospinning manufacturing step at 0 ~ 40kV on normal mask sheet; Nanofiber nonwoven skin care sheet manufacturing method comprising

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