KR20090002543A - Cosmetic composition comprising the extract of corylopsis coreana as active ingredient - Google Patents

Abstract

A cosmetic composition, and a method for using the composition to the skin are provided to improve the antiaging effect, the antioxidizing effect, the wrinkle improving effect and the whitening effect. A cosmetic composition contains an extract of Corylopsis coreana as an active ingredient. Preferably the content of the extract of Corylopsis coreana is 0.001-30.0 wt% based on the total composition. Preferably the extract of Corylopsis coreana is extracted by solvent extraction, supercritical extraction or ultrasonication extraction.

Description

Cosmetic composition containing hydroxy extract as an active ingredient {Cosmetic Composition Comprising the Extract of Corylopsis coreana as Active Ingredient}

The present invention relates to a skin external preparation composition excellent in antioxidant effect, anti-inflammatory effect, collagen biosynthesis effect, whitening effect, moisturizing effect containing hydroxy extract as an active ingredient.

As human skin ages, various changes occur. This is called aging, and there are various aging phenomena on human skin.

 The causes of aging include UV rays, mental stress, and the external environment. In this case, the most important role is reactive oxygen species, which in turn promotes adult disease and aging by damaging cells and tissues. More specifically, lipids, proteins, polysaccharides, and nucleic acids, which are major constituents of the skin, are oxidized to destroy skin cells and tissues, resulting in skin aging. In particular, the oxidation of proteins is caused by severe cuts in the connective tissues of collagen, hyaluronic acid, elastin, proteoglycan, fibronectin, and so on, resulting in severe over-inflammatory reaction and skin elasticity. If this worsens, mutations in the DNA can lead to mutations, cancer, and reduced immune function.

 In addition to reactive oxygen species, our skin suffers from aging. Therefore, in order to effectively prevent skin aging, it is very important to effectively remove external stimuli such as ultraviolet rays and to remove harmful substances such as free radicals generated in the skin.

 Most recently developed cosmetics are being researched to prevent external irritation and to remove skin harmful factors from the inside. In particular, many cosmetic products using natural products have been developed to reduce the skin irritation caused by various chemicals, etc. in order to exhibit such effects. Natural materials have less side effects on the skin, and as the consumer's response to cosmetics using natural materials has increased recently, the development value of cosmetics as a raw material for cosmetics is increasing.

The present inventors tried to develop anti-aging cosmetics by experimenting with anti-aging effects from various natural plants and effectively applying them to cosmetics in order to effectively prevent the phenomenon of skin aging phenomenon. In particular, in the present invention, to separate the plant having a variety of anti-aging efficacy to apply to the product.

Accordingly, it is an object of the present invention to provide a cosmetic composition containing a hydroxy extract having an antioxidant effect, an anti-aging effect, an anti-wrinkle effect, a whitening effect, a skin irritation-reducing effect and a moisturizing effect as an active ingredient.

The present invention provides a cosmetic composition containing a hydroxy extract having an antioxidant effect, an anti-aging effect, an anti-wrinkle effect, a whitening effect, a skin irritation-reducing effect and a moisturizing effect as an active ingredient.

The hyacinth ( Coylopsis coreana, Uyeki ) included in the cosmetic composition of the present invention is a plant of the locust family and is grown in Korea and grows in Namdo, Jiri, Suwon Gwanggyosan, and Sanbok. It grows up to 5m in height and forms a colony, and many neighbors rise up to form a large colony. It is not worn and is resistant to dryness and grows well in dry soil. Small branches are yellowish brown with white cortex on bark. The elliptical is oval yellowish brown. The leaves are regenerated, ovate, short apex, heart bottom, 5-9cm long, 4-8cm wide, with sharp teeth, the surface is light green, the texture is good, the back is grayish white, and there is no hair. Total inflorescence is 3-4cm long, grows to 7-8cm after flowering. In March, 8-12 small flowers hang in a lantern shape. Petals are obovate, light yellow-green.

As used herein, the term 'hiri extract' means that it is obtained by extracting from various organs or parts (eg, leaves, flowers, roots, stems, branches, shells and seeds, etc.) of the diary, preferably flowers, leaves, stems , Branches, bark or roots, more preferably flowers, leaves, branches or roots, most preferably extracts obtained from branches.

The hydroxy extract of the present invention may be prepared according to conventional methods known in the art, that is, using conventional solvents, under conditions of conventional temperature and pressure.

The hydroxy extract of the present invention uses at least one extraction solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol and hexane Extraction is performed using ethanol, 70% ethanol or water, and more preferably 70% ethanol.

On the other hand, it is apparent to those skilled in the art that the hydroxy extract of the present invention can be obtained in addition to the above-described extraction solvents, and extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-described extraction solvent, but also the extract that has undergone a conventional purification process. For example, decompression by carbon dioxide, extraction by supercritical extraction by high temperature, extraction by ultrasonic extraction, separation using an ultrafiltration membrane with a constant molecular weight cut-off value, various chromatography (size, charge, hydrophobicity or The active fraction obtained through various purification methods additionally performed, such as separation by affinity), is also included in the extract of the present invention.

 In addition, the present invention provides a cosmetic composition, characterized in that the extract is obtained by cold condensation at room temperature, heating and filtration, further obtained by concentrating the solvent under reduced pressure or lyophilization.

In addition, the hydroxy extract of the present invention is contained in 0.0001 to 30.0% by weight based on the entire cosmetic composition, preferably characterized in that it contains 0.001- 15.0% by weight. If the content of the extract is less than 0.0001% by weight does not appear to improve the skin, when more than 30.0% by weight of the effect of increasing the effect of the content is insignificant, there is a problem in the safety and stability of the formulation and is not economical.

  According to the present invention, the hydroxy extract has an antioxidant effect (Experimental Examples 1 and 2), MMP-1 production inhibitory effect (Experimental Example 3), MMP-1 production inhibitory effect by ultraviolet light (Experimental Example 4), collagen synthesis enhancement effect ( Experimental Example 5), melanin production inhibitory effect (Experimental Example 6), cytotoxicity mitigating effect by ultraviolet irradiation (Experimental Example 7), inflammatory cytokine expression inhibitory effect (Experimental Example 8), skin elasticity improvement effect ( Experimental Example 9) and excellent moisturizing effect (Experimental Example 10). Therefore, the cosmetic composition of the present invention comprising a hydroxy extract having various functions as an active ingredient has an antioxidant effect, an anti-aging effect, a wrinkle improvement effect, a whitening effect, a skin irritation relieving effect, and a moisturizing effect.

Ingredients included in the cosmetic composition of the present invention as an active ingredient includes components conventionally used in cosmetic compositions in addition to the active ingredient, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, And carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing , Oils, powder foundations, emulsion foundations, wax foundations and sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, and especially in the case of spray, additionally chlorofluorohydrocarbon, Propellant such as propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, the present invention provides a cosmetic method comprising applying a cosmetic composition containing the hydroxy extract of the present invention to human skin.

The cosmetic method of the present invention refers to all cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all the methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition with excellent skin protection effect according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towels and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

When the cosmetic composition including the hydroxy extract of the present invention is applied to human skin, anti-aging, anti-wrinkle effect, whitening effect, moisturizing effect, and skin irritation relief effect may be obtained.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Preparation Example 1: Preparation of hydroxy extract

The dried chopped hyacinth branch was refluxed three times for 5 hours with 70% (V / V) ethanol aqueous solution, cooled, and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and freeze dried at 50 ° C or lower. A hydroxy extract was prepared by dispersing / dissolving in a 30% butylene glycol solution under reduced pressure and freeze-dried 1%.

Experimental Example  One: NBT Antioxidant Effect Measurement Experiment

In this Experimental Example, in order to confirm the antioxidant effect of the hydroxy extract obtained in Preparation Example 1, another antioxidant BHT (Butylated Hydroxytoluene) was used as a comparative sample under laboratory conditions, and the antioxidant activity was measured using NBT method. In order to measure the antioxidant effect, the active oxygen produced by xanthine and xanthine oxidase is measured by the NBT method, and the effect of the test substance removing the active oxygen, that is, the active oxygen scavenging effect, is evaluated. Free radicals are produced by xanthine and xanthine oxidase. This active oxygen reacts with Nitro Blue Tetrazolium (NBT) to measure the active oxygen scavenging rate by measuring the blue color produced by this at a wavelength of 560 nm.

As a measuring method

0.05M Na ₂ CO ₃ ------------------------------------ 2.4ml

2.3mM xanthine solution ---------------------------------- 0.1ml

3.3mM EDTA Solution ------------------------------------ 0.1ml

4.BSA solution ---------------------------------------- 0.1ml

5.0.72 mM NBT solution ---------------------------------- 0.1ml

6.Xanthine Oxidase Solution ----------------------------- 0.1ml

7.6 mM CuCl ₂ solution ----------------------------------- 0.1ml

① Add 1,2,3,4,5 to Bayer's bottle and add 0.1ml of sample solution (concentration: 0.01, 0.1, 1.0%) and leave at 25 ℃ for 10 minutes.

② Add 6 and stir quickly, and start incubation for 20 minutes at 25 ℃.

③ After that, add 7 to stop the reaction and measure the absorbance St at 560nm.

④ For the blank test, measure the absorbance Bt by using distilled water instead of the sample solution as above.

⑤ Also, the blank of the sample solution is operated in the same manner using distilled water instead of 6 to measure the absorbance Bo.

The reactive oxygen scavenging ratio was calculated by Equation 1, and the results are shown in Table 1.

Free radical scavenging rate (%) = [1- (St-So) / (Bt-Bo)] X 100

St: absorbance at 560 nm after enzyme reaction of sample solution

Bt: absorbance at 560 nm after enzymatic reaction of blank test solution

So: absorbance at 560 nm before reaction without enzyme addition of sample solution

Bo: absorbance at 560 nm before reaction without enzyme in blank test solution

Sample Name Treatment concentration (%) Antioxidative effect(%) Diary extract 0.01 35.3 Diary extract 0.1 65.6 Diary extract 1.0 92.3 BHT 0.01 89.2

As shown in the results of Table 1, all of the samples tested at a concentration of 0.01-1.0% was able to confirm the excellent antioxidant effect. In addition, it was confirmed that the hydroxy extract has an excellent antioxidant power similar to BHT.

Experimental Example  2: DPPH Antioxidant Effect Measurement Experiment

In this Experimental Example, to measure the antioxidant effect of the hyacinth extract obtained in Preparation Example 1, the antioxidant activity such as vitamin C was compared in the laboratory conditions and the antioxidant activity was measured using the DPPH method.

The DPPH method measures the antioxidant activity by reducing power using a free group called DPPH (2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl) free radical. The free radical scavenging rate is measured at a wavelength of 560 nm by comparing the degree to which the DPPH is reduced by the test substance to reduce the absorbance.

As a reagent used, 2,2-Di (4-tert-octylphenyl) -1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 618.76) 0.1 mM solution was dissolved in methanol to make 100 ml.

As a measuring method

① Add 0.15ml of sample solution to 0.15ml of 0.1mM DPPH solution in 96-well plate, stir rapidly, and incubate for 10 minutes at 25 ℃.

(2) Then measure the absorbance St at 560nm.

③ In the blank test, measure the absorbance Bt by using distilled water instead of the sample solution as above.

④ Again, the blank of the sample solution was operated in the same manner using methanol instead of the 0.1 mM DPPH solution to measure the absorbance Bo.

The result of the effect was calculated by Equation 2, the results are shown in Table 2.

Free radical scavenging rate (%) = (1- (St-So) / (Bt-Bo)) X 100

St: absorbance at 560 nm after free radical scavenging of sample solution

Bt: absorbance at 560 nm after free radical scavenging of blank test solution

So: Absorbance at 560 nm before reaction without free radicals in sample solution

Bo: absorbance at 560 nm before reaction without free radicals in blank test solution

Sample Name Treatment concentration (%) Antioxidative effect(%) Diary extract 0.01 85.8 vit-C 0.01 86.1

As shown in the results of Table 2, the hydroxy extract of the present invention was confirmed to have an excellent free radical scavenging rate (antioxidant effect) similar to vitamin C at 0.01% concentration.

Experimental Example  3. MMP -1 suppression effect

Fibroblasts (Korean Cell Line Bank, Korea), which are human normal skin cells, were seeded in 48-well microplates (Nunc, Denmark) at 1 × 10 ⁶ cells per well, and treated with DMEM medium (Sigma, USA) and 37 ° C. After culturing for 24 hours at the hydroxy extract of Preparation Example 1 was further incubated for 48 hours in serum-free DMEM medium added to a final concentration of 100 ㎍ / ㎖ and serum-free DMEM medium containing no hydroxy extract as a control. After incubation, the supernatant of each well was collected and the amount of ng / ml newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA), and MMP-1 production was performed according to Equation 3 below. Inhibition rate was calculated and the results are shown in Table 3. At this time, TGF-β (10 ng / ml, Roche, United States) was used as a positive control of MMP-1 production inhibition rate.

MMP-1 production inhibition rate (%) = 1- (quantity of MMP-1 in the experimental group / amount of MMP-1 in the control group) × 100

Inhibition Rate of MMP-1 Formation of Diary Extracts (%) Test substance % Inhibition of MMP-1 production TGF-β 68.5 Preparation Example 1 (100 μg / ml) 75.5

As shown in the results of Table 3, the hydroxy extract of the present invention showed a degree of inhibition of MMP-1 production almost similar to TGF-β.

Experimental Example  4. Diary  By UV induction of extract MMP -1 suppression effect

In general, MMP-1 is known to increase its amount by ultraviolet light. Therefore, the experiment was conducted to find out whether the hydroxy extract of the present invention inhibits the amount of MMP-1, which is increased by ultraviolet rays. The experiment was performed in the same manner as in Experiment 3 except that a certain amount of UVA was treated before sample treatment. The extract used in this experiment was the extract of Preparation Example 1, but the results of this experiment are not limited thereto. MMP-1 production inhibition rate was calculated according to Equation 3, the results are shown in Table 4 below.

Inhibition rate of MMP-1 production by hydroxy extract after UV induction (%) Test substance % Inhibition of MMP-1 production TGF-β 75.3 Example 1 (100 μg / ml) 85.8

As shown in the results of Table 4, the inhibition rate of MMP-1 production after UV induction of the hydroxy extract of the present invention was better than that of TGF-β.

Experimental Example  5. Diary  Enhancement of Collagen Synthesis by Extracts

Fibroblasts, human normal epithelial cells, were seeded in 48-well microplates at 1 × 10 ⁶ cells per well and incubated in DMEM medium for 24 hours. Serum-free DMEM medium (control group) to which the hydroxy extract of Preparation Example 1 was added at a final concentration of 100 μg / ml and serum-free DMEM medium (control group) not containing the hydroxy extract were further incubated for 48 hours. After incubation, the supernatant of each well was collected and measured in ng / ml by measuring the amount of procollagen (procollagen) type I C-peptide (PICP) using a collagen kit (Takara, Japan), and the amount of collagen synthesized therefrom was measured. It was. TGF-β was used as a positive control in this experiment. Collagen biosynthesis increase rate was calculated according to the following equation (4), the results are shown in Table 5.

Collagen production increase rate (%) = (collage amount of experimental group / collagen amount of control group) × 100

Enhancement of Collagen Synthesis by Choy Extract Test substance Collagen biosynthesis rate (%) TGF-β 165.6 Example 1 (100 μg / ml) 183.8

As shown in the results of Table 5, the collagen synthesis enhancing effect of the hydroxy extract of the present invention was higher than that of TGF-β.

Experimental Example  6: Determination of Melanogenesis Inhibitory Effect Using B16F1 Melanogenic Cells

In order to confirm the whitening effect of the hydroxy extract obtained in Preparation Example 1, the whitening effect was determined by looking at the degree of inhibition of melanin production on B16F1 melanocytes. B16F1 melanocytes used in this experiment is a cell strain derived from the mouse, and secreted a black pigment called melanin. Samples were treated during the artificial culture of these cells to evaluate the degree of melanin black pigment reduction. B16F1 melanocytes used in the present experiment was used by receiving from the American Type Culture Collection (ATCC).

The melanin biosynthesis inhibitory effect of B16F1 melanogenesis cells was measured as follows. B16F1 melanocytes were dispensed in 6-well plates at a concentration of 2 X 10 ⁶ per well, and the cells were attached and incubated for 72 hours by treating the sample at a concentration that does not cause toxicity. After 72 hours of incubation, the cells were detached with trypsin-EDTA, the cell number was measured, and the cells were recovered by centrifugation. Quantification of intracellular melanin was carried out with a slight modification of the method of Rotan: Cancer Res., 40: 3345-3350, 1980. The cell pellet was washed once with PBS, and then 1 ml of homogenization buffer solution (50 mM sodium phosphate, pH 6.8, 1% Triton X-100, 2 mM PMSF) was added and vortexed for 5 minutes to disrupt cells. Melanin was extracted by adding 1N NaOH (10% DMSO) to the cell filtrate obtained by centrifugation (3,000 rpm, 10 minutes), and then the absorbance of melanin was measured at 405 nm with a microplate reader. Production inhibition rate (%) was measured. Melanin inhibition rate (%) of B16F1 melanogenesis cells was calculated by Equation 5, the values are shown in Table 6 below. IC ₅₀ value is the concentration of substance that inhibits melanin production by 50%. The same experiment was conducted using hydroquinone and arbutin as positive controls to confirm the degree of melanin inhibition rate of the hydroxy extract.

% Inhibition = [(A-B) / A] X100

A: Melanin amount in wells without sample

B: Melanin amount in the wells to which the sample was added

Sample Name Treatment concentration (%) Melanin Synthesis Inhibitory Effect (%) Heresy Extract (Manufacturing Example 1) 0.1 65.8 Hydroquinone 0.1 67.8 Arbutin 0.1 56.3

As shown in the results of Table 6, the melanin synthesis inhibitory effect of the hydroxy extract of the present invention was very high, showing a similar or superior effect compared to the existing whitening agents hydroquinone, arbutin and the like.

Example  7: Reduction of cytotoxicity by UV irradiation

This example was carried out to evaluate the mitigating effect of cytotoxicity by ultraviolet irradiation of the hydroxy extract obtained in Preparation Example 1. Fibroblasts (fibroblasts) were placed in a 24-well test plate each ⁵ × ¹ × ¹⁰ attached for 24 hours. Each well was washed once with PBS and 500 μl of PBS was added to each well. The fibroblasts were irradiated with UV 10mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UV B15W, Sankyo Dennki, Japan), and then PBS was removed and 10% FBS was added to the cell culture medium (DMEM). 1 ml) was added. Here the hydroxy extract to be treated was incubated for 24 hours. After 24 hours, the medium was removed, and 500 μl of cell culture medium and 60 μl of MTT solution (2.5 mg / ml) were added to each well, followed by incubation in a 37 ° C. CO ₂ incubator for 2 hours. The medium was removed, and 500 μl of isopropanol-HCl (0.04N) was added thereto. After shaking for 5 minutes, the cells were lysed and 100 μl of the supernatant was transferred to a 96-well test plate, and the absorbance was measured at 565 nm using a microplate reader. The cell viability (%) was measured by the following Equation 6, and the cytotoxicity relaxation rate by ultraviolet rays was calculated by the following Equation 7.

Cell survival rate (%) = [(St-Bo) / (Bt-Bo)] X 100

Bo: 565 nm absorbance of wells that only developed cell culture media

Bt: 565 nm absorbance of the wells that developed the colorless reaction wells

St: 565 nm absorbance of the wells that reacted the sample treated wells

Reduction rate of cytotoxicity by ultraviolet ray (%) = [1- (St-Bo) / (Bt-Bo)] X 100

Bo: cell viability of wells without UV irradiation and no sample treatment

Bt: cell viability of wells that were not irradiated with UV light

St: Cell survival rate of wells treated with UV

Cytotoxic alleviation rate (%) Sample Name Treatment concentration Cytotoxic alleviation rate (%) Hyacinth extract 0.1% 65 Hyacinth extract 0.5% 73

As shown in the results of Table 7, the hydroxy extract of the present invention suppresses cytotoxicity caused by ultraviolet rays at 65% at 0.1% concentration, and inhibits 73% at 0.25%, thereby effectively protecting cytotoxicity caused by ultraviolet rays. Could know.

Experimental Example  8: Inhibitory Effect of Inflammatory Cytokine Expression by Ultraviolet Irradiation

In this example, in order to evaluate the inhibitory effect of inflammatory cytokine expression expressed by ultraviolet irradiation of the hyacinth extract obtained in Preparation Example 1, fibroblasts isolated from human epidermal tissue (Fibroblast) were placed on a 24-well test plate ⁵ × 10 ^4. One by one and attached for 24 hours. Each well was washed once with PBS and 500 μl of PBS was added to each well. The fibroblasts were irradiated with UV 10mJ / cm 2 using an ultraviolet B (UVB) lamp (Model: F15T8, UV B 15W, Sankyo Dennki Co., Japan), after which the PBS was removed and the cell culture medium (FMEM was not added to DMEM). Medium) 350 μl was added. Here, the hydroxy extract to be treated was incubated for 5 hours. 150 μl of the culture supernatant was taken to quantify IL-1α to determine the inhibitory effect of inflammatory cytokine expression of the hyacinth extract. The amount of IL-1α was quantified using Enzyme-linked Immunosorbent Assay, and the production rate of IL-1α was calculated by Equation 8.

Inhibitory rate of inflammatory cytokine expression (%) = [1- (St-Bo) / (Bt-Bo)] X 100

Bo: IL-1α production in wells without UV irradiation

Bt: IL-1α production in wells that were not irradiated with UV light

St: IL-1α production amount of wells that were irradiated with UV light

Sample Name Treatment concentration (%) Inhibitory rate of inflammatory cytokine expression (%) Hyacinth extract 0.1% 53 Hyacinth extract 0.5% 69

As shown in the results of Table 8, the hydroxy extract of the present invention inhibits the production of inflammatory cytokines, IL-1α, by 58% at a concentration of 0.013% and inhibits 72% at 0.025%, resulting in low treatment. It can be seen that the concentration effectively protects against inflammation caused by ultraviolet rays.

Experimental Example  9. Diary  Containing extract Cosmetic  Skin elasticity improvement effect

In this Experimental Example, the cosmetic composition containing the hydroxy extract obtained in Preparation Example 1 (Example 1) was observed to improve skin elasticity in humans, and the cosmetic (Comparative Example 1) containing no hydroxy extract was prepared. The comparison was conducted and evaluated. The cosmetics used in the comparative experiments are in the form of a cream, the composition of which is shown in Table 9 below. 20 experimenters (20-35 year old female) were applied to the right side of the face cream of Example 1 and the left side of the face of the cream of Comparative Example 1 was applied twice a day for three consecutive months.

The skin elasticity improvement effect after the completion of the experiment was measured using a skin elasticity measuring instrument (cutometer SEM 575, C + K Electronic Co., Germany) before and after using the product for 2 months. Experimental results are described in Table 10 as ΔR8 value of Cutometer SEM 575, where R8 value represents the nature of the viscoelasticity of the skin.

ingredient Example 1 Comparative Example 1 Hydroxya extract lipophilic monostearate glycerine cetearyl alcohol stearic acid wax polysorbate 60 sorbitan stearate cured vegetable oil squalane mineral oil trioctanoin dimethicone sodium magnesium silicate glycerin betaine triethanolamine sodium hyaluronate preservative, fragrance, pigment distilled water 3.0 2.0 2.2 1.5 1.0 1.5 0.6 1.0 3.0 5.0 5.0 1.0 0.1 5.0 3.0 1.0 4.0 -2.0 2.2 1.5 1.0 1.5 0.6 1.0 3.0 5.0 5.0 1.0 0.1 5.0 3.0 1.0 4.0 Sum 100 100

The content units in the table are by weight.

Experimental product Skin elasticity effect (△ R8) Example 1 0.23 Comparative Example 1 0.09

As shown in the results of Table 10, it can be seen that the skin elasticity improving effect of the experimenter applying the cream containing the hydroxy extract is about 2 times better than the comparative group applying the cream containing the hydroxy extract.

Experimental Example  10. Diary  Containing extract Cosmetic  Moisturizing effect

In order to evaluate the moisturizing power of the cosmetics prepared in Experimental Example 9 (Example 1 and Comparative Example 1), the moisture retention ability of the skin to which each cosmetic was applied was measured as follows.

In the constant temperature and humidity room of 22 ° C. and 45% RH, each of the cosmetics of Examples and Comparative Examples was divided into half of 24 test subjects while applying the product of Example to one side and the product of Comparative Example to the other (2 times / day). ) The water content after 4 weeks was measured.

As a measuring device, a moisture content measuring instrument (Corneometer CM825, Courage + Khazaka, Germany) was used to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 11 below.

Water content Use week Example 1 Comparative Example 1 0 Week 23.61 ± 9.34 23.44 ± 11.36 4 Week 39.71 ± 7.06 24.28 ± 5.48 4 Week-0Week 16.10 ± 2.22 0.84 ± 5.88

As shown in the results of Table 11, the cosmetic composition containing the hydroxy extract of the present invention (Example 1) was significantly superior to the skin moisturizing effect compared to the cosmetic composition (Comparative Example 1) containing no hydroxy extract.

As described above, the hydroxy extract of the present invention has an excellent anti-aging effect such as antioxidant effect, MMP inhibitory effect, MMP expression control effect by UV irradiation, fine wrinkle improvement effect and the effect of relieving skin irritation caused by UV irradiation. Indicated. In addition, the hydroxy extract showed a whitening effect such as melanin production inhibitory effect, which is a substance causing blemishes, freckles and skin pigmentation. Therefore, it can be seen that the cosmetic composition containing the hydroxy extract has an excellent skin improvement effect such as an antioxidant effect, a collagen degrading enzyme control effect, a skin wrinkle improvement effect, an anti-inflammatory effect, and a whitening effect.

Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Thus, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.

Claims (5)

Antioxidant effect, anti-aging effect, skin wrinkle improvement effect, whitening effect, skin irritation relieving effect and moisturizing effect ( Corylopsis) coreana ) Cosmetic composition containing the extract as an active ingredient. The cosmetic composition according to claim 1, wherein the hydroxy extract is contained in an amount of 0.001-30.0 wt% based on the total composition. The extract of claim 1, wherein the hydroxy extract is selected from the group consisting of (a) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, chloroform, butyl acetate and 1,3-butylene glycol Cosmetic composition, characterized in that extracted by using a solvent extraction method, (b) supercritical extraction method or (c) ultrasonic extraction method using a solvent. According to claim 1, wherein the cosmetic composition is selected from cosmetic composition or ointment of the lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type or water-in-oil (W / O) type formulation. Cosmetic composition characterized in that. The cosmetic method according to any one of claims 1 to 4, wherein the cosmetic composition is applied to human skin.