KR20080109359A - Composition comprising the extract of crude drug complex for stimulating bone growth - Google Patents

Abstract

A pharmaceutical composition comprising the extracts of medicinal herbs is provided to accelerate the bone lengthening growth by using the natural product, thereby decreasing the cost and raising the absorption rate. The pharmaceutical composition for accelerating the bone lengthening growth comprises the extracts of medicinal herbs including 1-5(w/w) of Eucommiae Cortex, 1-5(w/w) of Astragali Radix and 1-3(w/w) of Angelica sinensis Diels, which are prepared with the water, alcohol or their mixture, as the active ingredient.

Description

Composition comprising the extract of crude drug complex for stimulating bone growth}

Figure 1 is a photograph taken by fluorescence microscopy of luminescence resulting from deposition on the deposited tibial epiphyseal growth plate after tetracycline injection in rats:

    A: saline administration group;

    B: growth hormone (20 µg / kg) administration group; And

    C: group administered with herbal extract extract (HT036, 500 mg / kg).

Figure 2 is a graph measuring the distance between the growth plate and the luminescence line 48 hours after tetracycline injection in rats:

^* : P <0.05; And

^*** : P <0.001.

3 is a graph showing growth rate in rats:

^* : P <0.05; And

^*** : P <0.001.

The present invention relates to a pharmaceutical composition for promoting bone length growth containing herbal extracts as an active ingredient, and more specifically, for promoting bone growth containing herbal extracts composed of larvae, Astragalus and Chinese Angelica as an active ingredient. It relates to a pharmaceutical composition.

Growth refers to the increase in height, and is promoted by nutrition and growth hormone. In particular, the nervous system, including the brain that secretes growth hormone, grows remarkably in childhood, and then grows slowly. Therefore, most of human growth occurs until the age of puberty, and if proper nutrition is not achieved at this time, the growth will not be done properly. In order to achieve nutritional balance, many growth-promoting health foods have been released, but only the combination of various ingredients helpful for growth has not yet reached a satisfactory level.

The growth of the long bones determines the height and skeleton of the body and is controlled by specific mechanisms. In particular, the growth of the epiphyseal growth plate of the iliac bone is the most important measure in the bone growth process. The growth plate is a resting zone before chondrocyte proliferation, a proliferation zone for proliferation, a maturation zone and hypertrophic zone for maturation and hypertrophy of chondrocytes. And their interactions lead to long bone growth (Loveridge, N, J. Anim. Sci. 77, 190-196, 1999).

Growth hormone required for growth has been used for the treatment of dwarfism patients for a long time.In late 1985, the recombinant growth hormone was commercialized, and mass production and supply became possible, and the risk of other diseases Recently, genetically modified growth hormone has been used as a treatment for growth hormone deficiency disease, and GHRP-6, a new growth hormone secretagogue consisting of 6 amino acids with growth hormone secretion activity based on enkephalin in vivo (Growth hormone releasing peptide-6, His-D-Trp-Ala-Trp-D-Phe-Lys-NH2) has been developed (Bowers CY. Et al., Endocrinology , 114, 1537-45, 1984), although Absorption rate, but has been reported to be absorbed upon oral administration (Noriko I. et al., Life Science , 47, 29-36, 1990). Subsequent studies continued with other peptide growth hormone secretion promoters, GHRP-1 (Ala-His-D-β-Nal-Ala-Trp-D-Phe-Lys-NH2) and GHRP-2 (D-Ala-D A growth hormone secretagogue consisting of six modified peptides, -β-Nal-Ala-Trp-D-Phe-Lys-NH2), has been developed and reported. However, they have been reported to have a lot of room for improvement in oral administration, although the activity in vivo is excellent but the absorption rate stays at 2-3% upon oral administration.

Eucommia is deciduous arborescent of Eucommia belonging to Eucommia Araliaceae (Eucommiaceae) (Eucommia The bark of ulmoides Oliver) is dried, and the bark of the cork is cut between spring and summer, and the bark is peeled off and dried in the sun. In oriental medicine, it was first described in the new agricultural herbal products , and in the herbal wood , it has been used to protect the liver and gods, strengthen the musculature , and stabilize the womb (Whang, WK et al., Kor. J. Pharmcogn , 27 (10), 65 -74, 1996). In addition, lowering blood pressure and stopping back pain has been used in combination with motherwort and prescription for the treatment of hypertension and has been widely used as a tonic, gangjeong, analgesic.

Recent pharmacological studies of larvae include anti-aging, anti-fatigue, glucosidase inhibitors, cardiac contractility, vasodilation, antidiabetic, analgesic and neuralgia, protein synthesis and lipid metabolism. Et al. (Li Y. et al., Biol. Pharm. Bull , 22 (6), 582-585, 1999). In addition, it has been reported that the application of osteoporosis-induced suppression has significant effects on bone resorption and prevention of bone mineral loss and bone angiogenesis, respectively, in the bark and leaves of the larvae (Oh, H. S, et al., J. of Herbology, 10 (1), 59-68, 1995).

Astragalus is a root barely stripped of the pericarp of the perennial herbaceous astragalus membranceus Bunge, which belongs to the leguminosae. Choline, betaine and folic acid. The benefits of traditional Korean medicine include boils, body weakness, jahan, boil, jigal, abdominal pain, kidney pain, diurnal disease, vulgaris and drainage pain.膿 止痛), food taste, loss of appetite, hemorrhoids, purpose, malaria, disinfection, uterine bleeding, gynecological disorders, menstrual irregularities, postpartum ectopic disease, expectorant water (祛痰 嗽), headache, septic deepening (潟肺 心火), dysentery and pediatric leukemia is effective. Literature includes hematopoietic activity (Ma R. et al., J Tradit Chin Med . Sep; 3 (3): 199-204, 1983), antioxidant activity (Lee Choon-young, Kangwon National University Research Institute of Agricultural Science 15: 103, 2004), rabbit Effect on osteoblast differentiation (Xu CJ. Et al., Zhong Nan Da Xue xue Bao Yi Xue Ban, Aug; 29 (4): 489-91, 2004), prevention of osteoporosis in ovarian red rats (Kim C) et al., Arch Pharm Res . Nov; 26 (11): 917-924, 2003), but have not been studied on the growth of the growth plate length.

Angelica gigas are classified as Angelica gigas Nakai, Angelica sinensis (Oliv.) Diels and Chinese Angelica (Angelica acutiloba (Sieb. & Zuc.) Kitagawa). Among them, Chinese Angelica is a Chinese herbal medicine that is widely used in Chinese medicine because of its efficacy in blood donation, landscape pain, and yunjojangjang. It acts on the system, on the immune system, analgesic, anti-inflammatory, antibacterial, hepatoprotective, anticancer and hematopoietic (Zu, KJ et al ,, Zhongyaotongbao , p.39, 1985). Chinese sugar ear ingredients include ligustilide, n-butylidenephthalide, vitamin B-12 and folic acid.

Thus, the inventors of the present invention in order to solve the problems such as high cost, low absorption rate and side effects of the growth hormone used to promote growth, while searching for natural products, the growth of the compound herbal extract containing tofu, Astragalus and Chinese Angelica subcutaneous injection The present invention has been completed by revealing that it has a superior effect on growth than hormones.

It is an object of the present invention to provide a pharmaceutical composition for promoting bone length growth, containing as an active ingredient an extract of a mixture consisting of larvae, Astragalus and Chinese Angelica.

In order to achieve the above object, the present invention provides a pharmaceutical composition for promoting bone length growth, containing the extract of the herbal medicine complex consisting of larvae, Astragalus and Chinese Angelica as an active ingredient.

In addition, the present invention provides a health supplement for promoting bone length growth containing the composition as an active ingredient.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

The present invention provides a pharmaceutical composition for promoting bone length growth, which contains the extract of the herbaceous complex, which consists of the larvae, Astragalus, and Chinese Angelica, as an active ingredient.

The herbal extract extract is dried and crushed tofu, Astragalus and Chinese Angelica, and then C ₁ to C, such as about 1 to 20 times the weight of the dried sample, preferably about 5 to 15 times the amount of water, ethanol, methanol and the like. ₄ lower alcohols or mixed solvents thereof having a mixing ratio (v / v) of about 1: 0.1 to 1:10, preferably 1: 0.2 to 1: 5, at room temperature for about 1 to 24 hours, preferably For 5 to 15 hours, most preferably for 6 hours. The extraction method is preferably hot water extraction, cold extraction, reflux cooling extraction or ultrasonic extraction, and more preferably, the crude extract which is the soluble extract of the present invention can be obtained by heating under reflux heating and concentrating under reduced pressure. The herbal extracts of soybean, Hwangki and Chinese donkey obtained in this way were named "HT036".

The composition ratio of the complex herbal extract of the present invention is preferably 1 to 5 parts by weight, 1 to 5 parts by weight of Astragalus, 1 to 3 parts by weight of Chinese Angelica, 1 to 3 parts by weight, 1 to 3 parts by weight of Astragalus, Chinese Angelica It is more preferable that it is 1-2 weight part, and it is most preferable that it is 2 weight part of crayfish, 2 weight part of Astragalus, and 1 weight part of Chinese Angelica.

In order to measure the growth of the herbal compound extract, rats were divided into three groups, a negative control group, an experimental group, and a positive control group, twice daily for four days, for 4 days, for the negative control group, and for the experimental group for the composite herbal extract (HT036, Examples 1 to 5). And Comparative Herbal Extracts (see Comparative Examples 1 to 6 and Table 2), respectively, orally, and positive controls were injected subcutaneously with growth hormone. Growth can be seen by the length of the tibial portion grown after the tetracycline (tetracycline) is deposited on the growth plate, it can be seen by measuring the distance between the growth plate and the light emitting color developed by the deposition of tetracycline. To this end, rats were intraperitoneally injected with tetracycline on day 3, and then rats were anesthetized with ether on day 5 after 48 hours.

After capturing the interval between the emission line and the growth plate deposited with tetracycline in the rat by fluorescence microscopy (see FIG. 1), growth was measured. As a result, the combination herbal extract (HT036) was shown to affect the bone length growth. The bone length growth of the HT036 administration group (see Examples 1 to 5 and Table 1) was statistically higher than that of the negative control group and the comparative medicinal extract administration group (see Comparative Examples 1 to 6 and Table 2). ^* Indicates P <0.05 and ^*** indicates P <0.001. In addition, when the growth rate of the negative control group administered with saline was 100%, the combination herbal extract (HT036, Examples 1 to 5 and Table 1) administration group, comparative medicinal extract (Comparative Examples 1 to 6 and Table 2) administration group and positive control group Calculate the growth rate of. As a result, the growth rate of the multi-drug extract extract (HT036, Examples 1 to 5 and Table 1) administration group was significantly higher than the negative control group, the comparative herbal extract administration group.

As a result of toxicity experiments by orally administering the above-mentioned herbal extract (HT036) to mice, 50% lethal dose (LD ₅₀ ) by oral toxicity test was determined to be a safe substance of at least 1,000 mg / kg or more.

The herbal compound extract (HT036) is a safe substance and can be provided as a pharmaceutical composition for promoting bone length growth because it shows the effect of excellent bone length growth.

The pharmaceutical composition for promoting bone length growth of the present invention preferably comprises 0.1 to 50% by weight of the herbal extract (HT036) based on the total weight of the composition, but is not limited thereto. The composition containing the herbal compound extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the preparation of the composition.

The pharmaceutical dosage forms of the herbal complex extracts of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable collection.

The compositions of the present invention can be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral formulations, external preparations, suppositories, and sterile injectable solutions. Carriers, excipients, and diluents that may be included in compositions containing wood vinegar include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents and surfactants are usually used.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose, and the like in the herbal compound extract. (sucrose), lactose (lactose), gelatin, etc. are mixed and prepared. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. .

Formulations for parenteral administration include sterile aqueous solutions, solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, suppositories, injections. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

Preferred dosages of the herbal extracts of the present invention vary depending on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration, and the duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the herbal extract of the present invention is preferably administered at 0.0001 to 1000 mg / kg, preferably 0.001 to 1000 mg / kg per day. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect.

The herbal complex extract of the present invention can be administered to various mammals such as mice, mice, livestock, humans. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

The herbal extract of the present invention is a composition that can be used with confidence even when taken for a long time because there is little toxicity and side effects.

In addition, it provides a dietary supplement for promoting bone length growth containing the composition of the present invention as an active ingredient.

Foods to which the herbal compound extract can be added include, for example, various foods, dairy products, beverages, gums, teas, vitamin complexes, dietary supplements, etc., and are used in the form of powders, granules, tablets, capsules, or beverages. Can be.

It may also be added to foods or beverages for the purpose of promoting growth. At this time, the amount of the extract in the food or beverage can be added in 0.01 to 15% by weight of the total food weight, the health beverage composition is added in a ratio of 0.02 to 5 g, preferably 0.3 to 1 g based on 100 ml Can be.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except the herbal compound extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks. have. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the complex herbal extract of the present invention may be used in various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors such as flavoring agents, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid And salts thereof, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the herbal extract of the present invention may contain the flesh for the production of natural fruit juices and fruit juice drinks and vegetable drinks. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the extract of the present invention.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples and Formulation Examples.

However, the following Examples, Experimental Examples, and Formulation Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples, Experimental Examples, and Formulation Examples.

<Examples 1 to 5> herbal medicine composite material Preparation of Extract (HT036)

After drying 400 g of Tochung, Hwanggi and Chinese donkeys, respectively, purchased from Gyeongdong Market (Seoul), the weight was measured and ground so as to have the composition and weight ratio as shown in Table 1. 70% ethanol (Examples 1 to 4) or distilled water (Example 5) corresponding to 10 times the weight of the sample was added thereto, followed by heating under reflux for 6 hours, followed by filtration under reduced pressure to separate the extract and the residue. After concentrating under reduced pressure using a vacuum rotary concentrator, each concentrated liquid was obtained. The resultant was frozen at -70 ° C, and then a powder was obtained using a freeze-dryer to obtain the yield of each medicine (Tofu 11.97%, Astragalus 24.63%). , Angelica 45.4%), lyophilized powder obtained according to the medicinal herb mixture ratio was used as a final extract (hereinafter referred to as "HT036") as a sample of the experiment.

Furtherance Tofu Astragalus Chinese donkey  Example 1 Weight (g) 40 40 20 Parts by weight 2 2 One  Example 2 Weight (g) 50 25 25 Parts by weight 2 One One  Example 3 Weight (g) 25 50 25 Parts by weight One 2 One  Example 4 Weight (g) 20 40 40 Parts by weight One 2 2  Example 5 Weight (g) 40 40 20 Parts by weight 2 2 One

<Comparative Examples 1 to 6> Comparative Herb Extract Preparation

The composition consisting of the composition ratio as shown in Table 2 below was prepared by extracting in the same manner as in Examples 1 to 4. In order to compare the growth effect of the present invention, growth hormone was used as a positive control, and saline was used as a negative control.

Furtherance Tofu Astragalus Chinese donkey  Comparative Example 1 Weight (g) 100 0 0 Parts by weight One 0 0  Comparative Example 2 Weight (g) 0 100 0 Parts by weight 0 One 0  Comparative Example 3 Weight (g) 0 0 100 Parts by weight 0 0 One  Comparative Example 4 Weight (g) 50 50 0 Parts by weight One One 0  Comparative Example 5 Weight (g) 50 0 50 Parts by weight One 0 One  Comparative Example 6 Weight (g) 0 50 50 Parts by weight 0 One One

<Experimental Example 1> herbal extracts (HT036) Measure of growth

For the measurement of growth by the herbal compound extract (HT036), the animals used in the experiment were Sprague-Dawley male rats (3 weeks old) purchased from Samtako (Korea) and used. The procedure was performed according to the National Institutes of Health animal care guidelines. Temperatures were unified under conditions of 20 ± 2 ° C and illumination between 07:00 and 19:00 hours, food and water were supplied via libitum, and rats were weighed daily.

The rats were divided into four groups of eight rats, saline solution for the negative control group, 500 mg / kg complex herbal extract (HT036, Examples 1 to 5 and Table 1) and 500 mg / kg comparative medicinal extract (comparison) Examples 1 to 6 and Table 2) were administered orally twice daily for 4 days, and growth hormone was injected subcutaneously at a concentration of 20 μg / kg in the positive control group, and on the third day, tetracycline (20 mg / kg) was intraperitoneally injected. Injection was made to deposit on the growth plate. On day 5 48 hours after tetracycline injection, rats were sacrificed by anesthesia with ether.

On the fifth day of the experiment, the tibia was isolated from the sacrificed rats, fixed in 4% phosphate-buffered paraformaldehyde for 48 hours, and then immersed in 30% sucrose solution. Thereafter, frozen bone tissue was frozen and cryocut was used to cut the sagittal sections of the tibia proximal part every 40 μm and collected and used as tissue samples. Sections were then used for length growth analysis.

In order to measure the growth, the distance between the growth plate and the emission line developed by tetracycline was measured by fluorescence microscopy (Hansson et al., Calcified Tissue Research, 10 (3), 238, 1972, FIG. 1), The average value for each section was calculated. All procedures were blinded by two observers. The results were expressed as mean ± standard deviation, and the difference between all groups was the Student-T test test and there was a significant difference in the standard deviation, but the Kruskall-Wallis test was not appropriate. Wallis test) was used.

As a result, the administration of the composite herbal extracts (Examples 1 to 5 and Table 1) resulted in a statistically significant growth than the negative control group and the comparative herbal extracts (Comparative Examples 1 to 6 and Table 2). Figure 2 shows the growth of the negative control group, positive control group, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Example 1 of the results of Table 3. ^* Indicates P <0.05 and ^** indicates P <0.01.

In addition, when the growth rate of the negative control group administered with saline was 100%, the combination herbal extract (HT036, Examples 1 to 5 and Table 1) administration group, comparative medicinal extract (Comparative Examples 1 to 6 and Table 2) administration group and positive control group Calculate the growth rate of. As a result, the growth rate of the multi-drug extract extract (HT036, Examples 1 to 5 and Table 1) administration group was significantly higher than the negative control group, the comparative herbal extract administration group. Figure 3 shows the growth rate of the negative control, positive control, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Example 1 of the results of Table 3. ^* Indicates P <0.05 and ^*** indicates P <0.001.

Growth and growth rate division Growth (μm / day) Growth Rate (%) ¹⁾ Negative control (saline) 348.12 ± 6.19 100 ± 1.78 Positive control (growth hormone) 380.89 ± 14.03 ^*** 109.41 ± 4.03 ^*** Example 1 374.32 ± 25.31 ^* 107.53 ± 7.27 ^* Example 2 372.01 ± 20.02 106.86 ± 5.81 Example 3 371.22 ± 15.06 106.64 ± 1.27 Example 4 371.45 ± 17.89 106.70 ± 5.19 Example 5 370.56 ± 15.64 106.45 ± 4.54 Comparative Example 1 353.46 ± 21.65 101.54 ± 6.47 Comparative Example 2 356.97 ± 30.25 102.54 ± 9.04 Comparative Example 3 365.97 ± 12.70 105.13 ± 3.65 Comparative Example 4 354.22 ± 40.21 101.75 ± 11.66 Comparative Example 5 355.26 ± 16.43 102.05 ± 4.76 Comparative Example 6 354.98 ± 23.05 101.97 ± 6.68

Statistical test: student-T test

^* : P <0.05

^*** : P <0.001

¹⁾ Formula = 100 + (Experimental Length Growth-Negative Control Length Growth) / Negative Control Length Growth × 100

Experimental Example 2 Medicinal Herb Composites Acute Toxicity Test of Extract (HT036)

In order to determine the acute toxicity of the herbal compound extract (HT036), the following experiment was performed.

SPF (specific pathogens free) ICR mice divided into 4 groups (3 males and 3 females / experimental group) at 4 weeks of age, temperature 22 ± 3 ° C, humidity 55 ± 10%, illumination 12L / 12D It was bred in a condition animal room. Mice were allowed to acclimate for about a week before being used in the experiment. Feed for experimental animals (for mice and rats, Dyets, USA) and negative water were supplied after sterilization and free intake.

The herbal compound extract (HT036) prepared in the above Example was prepared at a concentration of 50 mg / ml in 0.5% tween 80, and then 0.04 ml (100 mg / kg) and 0.2 ml (500 mg / kg) per 20 g of mouse body weight. Kg) and 0.4 ml (1,000 mg / kg). Samples were administered orally once and observed for side effects or lethality for 7 days after administration. That is, on the day of administration, changes in general symptoms and the presence or absence of dead animals were observed at least 1 hour, 4 hours, 8 hours, 12 hours after the administration, and at least once every morning and afternoon from the day after the administration. In addition, on the 7th day of administration, animals were killed and dissected, and the internal organs were visually examined. The change in body weight was measured at daily intervals from the day of administration, and the weight loss phenomenon of the animals by the herbal extract (HT036) was observed.

As a result, no significant clinical symptoms were observed in all the mice treated with the sample, no mice died, and no toxicity change was observed in weight change, blood test, blood biochemistry test, autopsy findings, etc.

Therefore, the herbal compound extract (HT036) did not show toxicological changes up to 1,000 mg / kg in all mice, and it was determined that the oral administration minimum dose (LD ₅₀ ) was more than 1,000 mg / kg.

Examples of preparations for the compositions of the present invention are illustrated below.

Preparation Example 1 Preparation of Pharmaceutical Formulation

<1-1> Preparation of Injection

Medicinal Herb Extract (HT036) 10 mg

Sodium Metabisulfite 3.0 mg

Methylparaben 0.8 mg

Propylparaben 0.1 mg

Appropriate sterile distilled water for injection

The above ingredients are mixed and made into 2 ml by a conventional method, and then filled into 2 ml ampoules and sterilized to prepare an injection.

<1-2> Preparation of Tablet

Medicinal Herb Extract (HT036) 200 mg

Lactose 100 mg

Starch 100 mg

Magnesium stearate proper amount

The above components are mixed and tableted according to a conventional method for producing tablets to produce tablets.

<1-3> Preparation of Capsule

Medicinal Herb Extract (HT036) 10 mg

Lactose 50 mg

Starch 50 mg

Talc 2 mg

2 mg magnesium stearate

Capsules are prepared by mixing the above ingredients and filling into gelatin capsules according to a conventional method for preparing capsules.

<1-4> Preparation of Liquid

Medicinal Herb Extract (HT036) 1000 mg

20 g of sugar

20 g of isomerized sugar

Lemon flavor

Purified water was added to adjust the total volume to 1000 ml. According to the conventional method for preparing a liquid, the above components are mixed, and then filled into a brown bottle and sterilized to prepare a liquid.

Preparation Example 2 Preparation of Food

<2-1> Preparation of Health Supplements

Medicinal Herb Extract (HT036) 1000 mg

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

50 μg folic acid

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. It can be used in the manufacture of health food compositions (eg nutritional candy, etc.) according to conventional methods.

<2-2> Preparation of Flour Food

Herb compound composite extract (HT036) 0.1 ~ 10.0 parts by weight was added to the flour, and using the mixture to prepare bread, cake, cookies, crackers and noodles in a conventional manner to prepare a food for health promotion.

<2-3> Preparation of Soups and Gravys

Herb compound composite extract (HT036) 0.1 ~ 1.0 parts by weight was added to soups and broth to prepare meat products for health promotion, soup of noodles and broth in a conventional manner.

<2-4> Preparation of Ground Beef

10 parts by weight of the herbal compound extract (HT036) was added to the ground beef to prepare a ground beef for health promotion in a conventional manner.

<2-5> Production of Dairy Products

Herb compound composite extract (HT036) 0.1 ~ 1.0 parts by weight was added to the milk, using the milk to prepare a variety of dairy products such as butter and ice cream in a conventional manner.

<2-6> Preparation of Wire

Brown rice, barley, glutinous rice, and yulmu were alphad by a known method, and then dried and roasted to prepare a powder having a particle size of 60 mesh.

Black beans, black sesame seeds, and perilla were also steamed and dried by a known method, and then ground to a powder having a particle size of 60 mesh.

The herbal compound extract (HT036) was decompressed and concentrated in a vacuum concentrator, and dried by spraying and drying with a hot air dryer.

The dry powder of the grains, seeds and herbal extracts (HT036) prepared above was blended in the following ratio to prepare a conventional method.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight perilla, 8 parts by weight black beans, 7 parts by weight black sesame seeds),

Dry powder (1 part by weight) of the herbal extract extract (HT036),

Ganoderma lucidum (0.5 parts by weight),

Foxglove (0.5 part by weight)

Preparation Example 3 Preparation of Beverage

<3-1> Preparation of Healthy Drinks

Medicinal Herb Extract (HT036) 1000 mg

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components in accordance with the conventional healthy beverage production method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored Used to prepare the healthy beverage composition of the invention.

Although the composition ratio is a composition suitable for a preferred beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, use purpose.

<3-2> Preparation of Vegetable Juice

0.5 g of the herbal compound extract (HT036) was added to 1,000 ml of a vegetable juice such as tomato or carrot to prepare a vegetable juice for health promotion in a conventional manner.

<3-3> Preparation of fruit juice

0.1 g of the herbal compound extract (HT036) was added to 1,000 ml of fruit juice such as apple or grape, thereby preparing a fruit juice for health promotion in a conventional manner.

As described above, the herbal extract of the present invention composed of the larvae, Astragalus and Chinese Angelica extract exhibits an effect on growth, and can be used as a dietary supplement for promoting bone length growth as well as as a dietary supplement.

Claims (9)

A pharmaceutical composition for promoting bone length growth, comprising an extract of a herbal compound consisting of tongchung, hwanggi and Chinese donkeys as an active ingredient. According to claim 1, wherein the herbal extract extract is a medicinal composition for promoting bone growth, characterized in that extracted from the herbal composition consisting of chungchung 1 ~ 5: Astragalus 1 ~ 5: Chinese Angelica 1 ~ 3 (w / w) . The method of claim 2, wherein the herbal extract extract is a medicinal composition for promoting bone growth, characterized in that the extract is composed from the Chinese herbal medicine 1 ~ 3: Astragalus 1 ~ 3: Chinese Angelica 1 ~ 2 (w / w) . The pharmaceutical composition for promoting bone growth according to claim 3, wherein the herbal compound extract is extracted from the herbal compound consisting of Tohgi 2: Astragalus 2: Chinese Angelica 1 (w / w). The pharmaceutical composition for promoting bone growth according to claim 1, wherein the herbal extract is extracted with water, alcohol or a mixture thereof. 6. The pharmaceutical composition for promoting bone growth according to claim 5, wherein the alcohol is C ₁ to C ₄ lower alcohols. 7. The pharmaceutical composition for promoting bone growth according to claim 6, wherein the lower alcohol is ethanol. 8. The pharmaceutical composition for promoting bone length growth according to claim 7, wherein the ethanol is heated at reflux for 6 hours. Bone length growth promoting health supplement containing the composition of claim 1 as an active ingredient.

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