KR100818204B1 - Composition comprising the extract of viscum album var. coloratum for stimulating bone growth - Google Patents

Abstract

An extract of Viscum album var. coloratum is provided to promote the growth of bone length effectively, thereby being usefully used as a pharmaceutical composition and a health supplement food for promoting the bone length growth. A pharmaceutical composition for promoting bone growth comprises an extract of Viscum album var. coloratum, which is prepared by extracting leaves or stems of the Viscum album var. coloratum with water, C1-4 alcohol or a mixture thereof as a solvent for 1-24 hours. Further, an added weight of the solvent is 1 to 10 times as large as the Viscum album var. coloratum.

Description

Composition for promoting bone length growth containing mistletoe extract as an active ingredient {Composition comprising the extract of Viscum album var. coloratum for stimulating bone growth}

The present invention relates to a bone length growth promoting composition containing the mistletoe extract as an active ingredient, more specifically, bone length growth containing the mistletoe extract extracted with water, alcohol, or a mixture thereof as a solvent as an active ingredient. It relates to a composition for promotion.

Growth refers to the increase in height, and is promoted by nutrition and growth hormone. In particular, the nervous system, including the brain that secretes growth hormone, grows remarkably in childhood, and then grows slowly. Therefore, most of human growth occurs until the age of puberty, and if proper nutrition is not achieved at this time, the growth will not be done properly. In order to achieve nutritional balance, many growth-promoting health foods have been released, but only the combination of various ingredients helpful for growth has not yet reached a satisfactory level.

The growth of the long bones determines the height and skeleton of the body and is controlled by specific mechanisms. In particular, the growth of the epiphyseal growth plate of the iliac bone is the most important measure in the bone growth process. The growth plate is a resting zone before proliferation of chondrocytes, a proliferation zone for proliferation, and a maturation zone and hypertrophic zone for maturation and hypertrophy of chondrocytes. And their interactions result in long bone growth (Loveridge, N., J. Anim . Sci . 77, 190-196, 1999).

Growth hormone required for growth has been used for the treatment of dwarfism patients for a long time.In late 1985, the recombinant growth hormone was commercialized, and mass production and supply became possible, and the risk of other diseases Recently, genetically modified growth hormone has been used as a therapeutic agent for growth hormone deficiency disease, and GHRP-6, a new growth hormone secretagogue consisting of 6 amino acids with growth hormone secretion activity based on enkephalin in vivo (Growth hormone releasing peptide-6, His-D-Trp-Ala-Trp-D-Phe-Lys-NH2) has been developed (Bowers CY. Et al., Endocrinology , 114, 1537-45, 1984), although Absorption rate, but has been reported to be absorbed upon oral administration (Noriko I. et. al ., Life Science , 47, 29-36, 1990). Subsequent research has led to other peptide growth hormone secretion promoters, GHRP-1 (Ala-His-D-β-Nal-Ala-Trp-D-Phe-Lys-NH2) and GHRP-2 (D-Ala-D A growth hormone secretagogue consisting of six modified peptides, -β-Nal-Ala-Trp-D-Phe-Lys-NH2), has been developed and reported. However, they have been reported to have a lot of room for improvement in oral administration, although the activity in vivo is excellent but the absorption rate stays at 2-3% upon oral administration.

Mistletoe ( Viscum album var . coloratum ) is a perennial semiparasitic plant belonging to the mistletoe family, and is parasitic on various kinds of plants such as oak and mulberry.In oriental medicine, pain relief such as back pain and toothache, improvement of blood circulation such as hypertension and arteriosclerosis, prevention of miscarriage and removal of tumor It is used as a food material such as tea, which is lightly sweetened with its stem or leaves, or used as a medicinal herb after heat treatment for a long time.

Since ancient times in Europe, it has been used as a folk remedy for epilepsy, infertility and hypertension, and since it was proposed as a cancer treatment by Rudolf Steiner in Germany in the 1920s, it stimulates the immune system of patients before and after surgery to relapse secondary tumors. Membrane has been used as an anti-cancer immunotherapy, or for treating adult diseases such as hypertension and arteriosclerosis. In particular, it is widely used in anticancer immunotherapy, which is due to the excellent cell killing effect and the immune-promoting effect of mistletoe, and is most active in biscotoxins, alkaloids and polysaccharides and mistletoe. It is known that the effect is maintained by the lectins which are the components shown. Literature has shown anticancer activity (Sagar S M. et. al ., Curr Oncol . Feb; 13 (1): 14-26, 2006), cholesterol control (Ben E E. et al ., J Physiol Sci . Jun; 21 (1-2): 55-60, 2006). Mistletoe-related patents include a novel anticancer lectin of Korean mistletoe and a method for isolating genes and lectins, and a cosmetic composition that emphasizes the expression of heat shock proteins of application number 2000-0083067. There is a whitening agent comprising the above extract of 2001-0020355, there is an immunostimulant composition containing the Korean mistletoe extract of the application No. 1996-0005732, there is a mistletoe extract having anti-inflammatory and antioxidant activity of the application No. 2004-0074222, but Until now, the effect on the growth of the length of the growth plate has not been studied.

Thus, the present inventors completed the present invention by revealing that the mistletoe extract has an excellent effect on growth while searching for natural products, in order to solve problems such as high cost, low absorption rate and side effects of the growth hormone used to promote growth. .

An object of the present invention to provide a composition for promoting bone length growth containing mistletoe extract as an active ingredient.

In order to achieve the above object, the present invention provides a pharmaceutical composition for promoting bone length growth containing the mistletoe extract as an active ingredient.

The present invention also provides a healthy food for bone length growth containing the composition as an active ingredient.

Mistletoe extract of the present invention shows an effect on bone length growth, can be used in the pharmaceutical composition for promoting bone length growth as well as health food.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

Mistletoe extract is an active ingredient of the present invention is prepared by a manufacturing method comprising the following steps.

1) extracting the dried mistletoe with the extraction solvent; And

2) filtering and concentrating the mistletoe extract of step 1),

In the above production method, the mistletoe of step 1) can be used both stem and leaves, can be used without limitation, such as cultivated or commercially available.

In the above production method, the extraction solvent of step 1) is characterized in that the water, alcohol or a mixture thereof. The alcohol is preferred to use a C ₁ to C ₄ lower alcohol and the lower alcohol is preferably an ethanol. The extraction solvent is preferably extracted by adding 5 to 15 times the amount of dried mistletoe, more preferably by adding 10 times, and most preferably by adding 5 times, but not always limited thereto. It is preferable that the temperature of the water of the water bath during extraction is 60 to 100 ° C, more preferably 80 ° C, but is not limited thereto. In addition, the extraction time is preferably 1 to 24 hours, more preferably 5 hours to 15 hours, and most preferably 6 hours is not limited thereto. In addition, the extraction number is preferably 1 to 5 times, more preferably 4 times repeated extraction is not limited thereto. As the extraction method, hot water extraction, cold needle extraction, reflux cooling extraction, reflux heating extraction or ultrasonic extraction can be used. In the present invention, the mistletoe crude extract, which is a soluble extract of the present invention, can be obtained by condensing under reduced pressure after reflux heating extraction. .

The present invention provides a pharmaceutical composition for promoting bone length growth, which contains the mistletoe extract prepared by the preparation method as an active ingredient.

In the present invention, in order to measure the effect of the mistletoe extract on bone length growth, the rats were divided into a total of five groups, a negative control group, three experimental groups for each solvent, and a positive control group, and saline to the negative control group twice a day for four days. For M, mistletoe water extract, ethanol extract and methanol extract were orally administered, respectively, and the positive control group was injected subcutaneously with growth hormone. Growth can be seen by the length of the tibial portion grown after the tetracycline (tetracycline) is deposited on the growth plate, it can be seen by measuring the distance between the growth plate and the light emitting color developed by the deposition of tetracycline. To this end, rats were intraperitoneally injected with tetracycline on day 3 and then rats were sacrificed with ether anesthesia on day 5 after 48 hours. After capturing the interval between the emission line and the growth plate deposited with tetracycline in the rat by fluorescence microscopy (see FIG. 1), growth was measured.

As a result, mistletoe extract was found to affect bone length growth. The bone length growth of the mistletoe extract group showed a statistically significant growth effect compared to the bone length growth of the negative control group. ^* Represents P <0.05. In addition, assuming 100% growth of the negative control group administered with saline, the bone length growth rate of the experimental group administered the mistletoe extract was higher than this (see Table 1 and Figure 3). Therefore, the effect of the mistletoe extract on the growth is excellent.

As a result of oral administration of the mistletoe extract to mice, 50% lethal dose (LD ₅₀ ) by oral toxicity test was determined to be a safe substance of at least 1,000 mg / kg or more.

Mistletoe extract is a safe substance, and because it shows an excellent effect of bone length growth can be provided as a pharmaceutical composition for promoting bone length growth.

The pharmaceutical composition for promoting bone length growth of the present invention preferably includes 0.1 to 50% by weight of the mistletoe extract based on the total weight of the composition, but is not limited thereto. The composition containing the mistletoe extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the preparation of the composition.

The pharmaceutical dosage forms of the mistletoe extract of the present invention may be used in the form of their pharmaceutically acceptable salts, and may be used alone or in combination with other pharmaceutically active compounds, as well as in a suitable collection.

The compositions of the present invention can be used in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral formulations, external preparations, suppositories, and sterile injectable solutions. Carriers, excipients and diluents that may be included in the composition containing wood vinegar include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate , Cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate and mineral oil. When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used.

Solid preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, and the solid preparations may include at least one excipient such as starch, calcium carbonate, sucrose ( It is prepared by mixing sucrose or lactose and gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. .

Formulations for parenteral administration include sterile aqueous solutions, solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, suppositories, injections. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The preferred dosage of the mistletoe extract of the present invention depends on the condition and weight of the patient, the extent of the disease, the form of the drug, the route of administration and the duration, and may be appropriately selected by those skilled in the art. However, for the preferred effect, the herbal extract of the present invention is preferably administered at 0.0001 to 100 mg / kg, preferably at 0.001 to 100 mg / kg. Administration may be administered once a day or may be divided several times. The dosage does not limit the scope of the invention in any aspect. Mistletoe extract of the present invention can be administered to various mammals such as mice, mice, livestock, humans. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

Mistletoe extract of the present invention is a composition that can be used safely even when taken for a long time because there is little toxicity and side effects.

In addition, the present invention provides a healthy food for bone length growth containing the composition of the present invention as an active ingredient.

Foods to which the mistletoe extract may be added include, for example, various foods, dairy products, beverages, gums, teas, vitamin mono- and dietary supplements, and can be used in the form of powders, granules, tablets, capsules or beverages. have.

It may also be added to foods or beverages for the purpose of promoting growth. At this time, the amount of the extract in the food or beverage can be added in 0.01 to 15% by weight of the total food weight, the health beverage composition is added in a ratio of 0.02 to 5 g, preferably 0.3 to 1 g based on 100 ml Can be.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for containing the herbal extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in general beverages. . Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (tauumatin, stevia extract (for example, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of said natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the mistletoe extract of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese, chocolate), pectic acid and salts thereof, alginic acid and Salts, organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks, and the like. Others may contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected in the range of 0.1 to about 20 parts by weight per 100 parts by weight of the extract of the present invention.

Hereinafter, the present invention will be described in detail by Examples, Experimental Examples and Formulation Examples.

However, the following Examples, Experimental Examples, and Formulation Examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following Examples, Experimental Examples, and Formulation Examples.

< Example > Preparation of Mistletoe Extract

<1-1> ethanol aqueous solution extract

After drying the mistletoe purchased from Gyeongdong Market (Seoul), 2L of 70% ethanol corresponding to 10 times the weight of the sample was added to 200 g, and the mixture was extracted by heating under reflux for 6 hours at 85 ° C., followed by filtration under reduced pressure to separate the extract and the residue. Thereafter, the resultant was concentrated under reduced pressure using a reduced pressure rotary concentrator to obtain respective concentrates. After freezing at -70 ° C, the powder was obtained using a freeze dryer (54.5 g) (yield: 27.23%), and the final extract was extracted. It was used as a sample of the following experiment.

<1-2> water extract

In the method of <1-1>, water was added instead of 70% ethanol and extracted at 100 ° C. to obtain 47.0 g (yield: 23.52%).

<1-3> methanol extract

In the method of <1-1>, 100% methanol was added instead of 70% ethanol and extracted at 75 ° C. to obtain 57.4 g (yield: 28.71%).

< Experimental Example  1> caused by mistletoe extract Measure of growth

In order to measure the growth by mistletoe extract, the animals used in the experiment were Sprague-Dawley male rats (3 weeks old) purchased from Samtako (Korea), and the experimental procedure was US National. It was performed according to the animal care guidelines of the health center. Temperatures were unified under conditions of 20 ± 2 ° C and illumination between 07:00 and 19:00 hours, food and water were supplied via libitum, and rats were weighed daily.

The rats were divided into five groups of 10 rats, and saline solution was administered to the negative control group, and 100 mg / kg of mistletoe ethanol aqueous solution, water extract, and methanol extract were administered orally twice daily for 4 days. Was injected subcutaneously at a concentration of 20 μg / kg, and on day 3, tetracycline (20 mg / kg) was intraperitoneally injected to deposit on the growth plate. On day 5 48 hours after tetracycline injection, rats were sacrificed by anesthesia with ether.

On the fifth day of the experiment, the tibia was isolated from the sacrificed rats, fixed in 4% phosphate-buffered paraformaldehyde for 48 hours, and then immersed in 30% sucrose solution. Thereafter, frozen bone tissue was frozen and cryocut was used to cut the sagittal sections of the tibia proximal part every 40 μm and collected and used as tissue samples. Sections were then used for length growth analysis.

For the measurement of growth, the distance between the growth plate and the emission line developed by tetracycline was measured by fluorescence microscopy (Hansson et al. al ., Calcified Tissue Research , 10 (3), 238, 1972, Fig. 1), the average value for each section was calculated. All procedures were blinded by two observers. The results were expressed as mean ± standard deviation, and the difference between all groups was the Student-T test test and there was a significant difference in the standard deviation, but the Kruskall-Wallis test was not appropriate. Wallis test) was used.

As a result, all mistletoe extract groups showed statistically significant bone growth efficacy than the negative control group (see Table 1). Figure 2 shows the growth of the negative control, positive control, Example <1-1> of the results in Table 1. ^{* Indicates} P <0.05, ^** indicates P <0.01.

In addition, when the growth rate of the negative control group administered with saline was 100%, the growth rate of the mistletoe extract administration group, the positive control group was calculated. As a result, the growth rate of all mistletoe extract group was significantly higher than the negative control group.

Figure 3 shows the growth rate of the negative control, positive control, Example <1-1> of the results in Table 1. ^{* Indicates} P <0.05, ^** indicates P <0.01.

Growth and growth rate division Growth (μm / day) % Growth Negative control (saline) 305.12 ± 23.40 100 ± 7.67 Positive control (growth hormone) 344.91 ± 11.85 ** 113.04 ± 3.88 ** Example <1-1> 342.87 ± 12.08 * 112.37 ± 3.96 * Example <1-2> 332.64 ± 10.12 * 109.01 ± 3.86 * Example <1-3> 338.35 ± 11.24 * 110.89 ± 3.92 *

Statistical test: student-T test

^* : P <0.05

^** : P <0.01

¹⁾ Formula = 100 + (Experimental Length Growth-Negative Control Length Growth) / Negative Control Length Growth × 100

< Experimental Example  2> Acute Toxicity Test of Mistletoe Extracts

To determine the acute toxicity of mistletoe extracts, the following experiment was performed.

SPF (specific pathogens free) ICR mice were divided into 10 groups (3 males and 3 females / experimental group) at 4 weeks of age, temperature 22 ± 3 ℃, humidity 55 ± 10%, illumination 12L / 12D It was bred in a condition animal room. Mice were allowed to acclimate for about a week before being used in the experiment. Feed for experimental animals (for mice and rats, Dyets, USA) and negative water were supplied after sterilization and free intake.

The mistletoe ethanol aqueous solution extract, water extract and methanol extract prepared in the above example were prepared at a concentration of 50 mg / ml in 0.5% tween 80, respectively, and then 0.04 ml (100 mg / kg), 0.2 per 20 g of mouse body weight. Oral (mL) (500 mg / kg) and 0.4 mL (1,000 mg / kg). Samples were administered orally once and observed for side effects or lethality for 7 days after administration. That is, on the day of administration, changes in general symptoms and the presence or absence of dead animals were observed at least 1 hour, 4 hours, 8 hours, 12 hours after the administration, and at least once every morning and afternoon from the day after the administration. In addition, on the 7th day of administration, animals were killed and dissected, and the internal organs were visually examined. The change in body weight was measured at the interval of 1 day from the day of administration, and the weight loss phenomenon of the animals by the mistletoe extract was observed.

As a result, no significant clinical symptoms were observed in all the mice treated with the sample, no mice died, and no toxicity change was observed in weight change, blood test, blood biochemistry test, autopsy findings, etc. Therefore, mistletoe extracts did not show toxicological changes up to 1,000 mg / kg in all mice, and it was judged as a safe substance with an oral administration minimum dose (LD50) of 1,000 mg / kg or more.

Examples of preparations for the compositions of the present invention are illustrated below.

< Formulation example  1> Preparation of Pharmaceutical Formulations

<1-1> Preparation of Injection

10 mg of extract of Example <1-1>

Sodium Metabisulfite 3.0 mg

Methylparaben 0.8 mg

Propylparaben 0.1 mg

Appropriate sterile distilled water for injection

The above ingredients are mixed and made into 2 ml by a conventional method, and then filled into 2 ml ampoules and sterilized to prepare an injection.

<1-2> Preparation of Tablet

200 mg of extract of Example <1-1>

Lactose 100 mg

Starch 100 mg

Magnesium stearate proper amount

The above components are mixed and tableted according to a conventional method for producing tablets to produce tablets.

<1-3> Preparation of Capsule

10 mg of extract of Example <1-1>

Lactose 50 mg

Starch 50 mg

Talc 2 mg

2 mg magnesium stearate

Capsules are prepared by mixing the above ingredients and filling into gelatin capsules according to a conventional method for preparing capsules.

<1-4> Liquid  Produce

1000 mg of extract of Example <1-1>

20 g of sugar

20 g of isomerized sugar

Lemon flavor

Purified water was added to adjust the total volume to 1000 ml. According to the conventional method for preparing a liquid, the above components are mixed, and then filled into a brown bottle and sterilized to prepare a liquid.

< Formulation example  2> Manufacture of food

<2-1> Preparation of health food

1000 mg of extract of Example <1-1>

Vitamin mixture proper amount

70 μg of Vitamin A Acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

Vitamin B6 0.5 mg

0.2 μg of vitamin B12

Vitamin C 10 mg

10 μg biotin

Nicotinic Acid 1.7 mg

50 μg folic acid

Calcium Pantothenate 0.5mg

Mineral mixture

Ferrous Sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

Potassium monophosphate 15 mg

Dibasic calcium phosphate 55 mg

Potassium Citrate 90 mg

Calcium Carbonate 100 mg

Magnesium chloride 24.8 mg

Although the composition ratio of the above-mentioned vitamin and mineral mixtures is mixed with a component suitable for a health food in a preferred embodiment, the compounding ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method. It can be used in the manufacture of health food compositions (eg nutritional candy, etc.) according to conventional methods.

<2-2> Preparation of Flour Food

0.1-10.0 parts by weight of the extract of Example <1-1> was added to flour, and bread, cake, cookies, crackers and noodles were prepared in a conventional manner using the mixture to prepare foods for health promotion.

<2-3> soup  And juicy ( gravies Manufacturing

0.1 ~ 1.0 parts by weight of the extract of Example <1-1> was added to soups and broth to prepare meat products for health promotion, soups of noodles and broth in a conventional manner.

<2-4> ground Ground beef  Produce

10 parts by weight of the extract of Example <1-1> was added to ground beef to prepare a ground beef for health promotion in a conventional manner.

<2-5> Dairy Products ( dairy products Manufacturing

0.1 to 1.0 parts by weight of the extract of Example <1-1> was added to milk, and various dairy products such as butter and ice cream were prepared in a conventional manner using the milk.

<2-6> Linear  Produce

Brown rice, barley, glutinous rice, and yulmu were alphad by a known method, and then dried and roasted to prepare a powder having a particle size of 60 mesh.

Black beans, black sesame seeds, and perilla were also steamed and dried by a known method, and then ground to a powder having a particle size of 60 mesh.

The extract of Example <1-1> was depressurized and concentrated in a vacuum concentrator, and the dried product obtained by drying with a sprayer and a hot air dryer was pulverized with a particle size of 60 mesh to obtain a dry powder.

The dry powders of the grains, seeds and extracts of Example <1-1> prepared above were blended in the following ratios to prepare a conventional method.

Cereals (30 parts by weight brown rice, 15 parts by weight brittle, 20 parts by weight of barley),

Seeds (7 parts by weight perilla, 8 parts by weight black beans, 7 parts by weight black sesame seeds),

Dry powder (1 part by weight) of the extract of Example <1-1>,

Ganoderma lucidum (0.5 parts by weight),

Foxglove (0.5 part by weight)

< Formulation example  3> Manufacture of beverage

<3-1> Preparation of Healthy Drinks

1000 mg of extract of Example <1-1>

Citric acid 1000 mg

100 g oligosaccharides

Plum concentrate 2 g

1 g of taurine

Add 900 ml of purified water

After mixing the above components according to the conventional healthy beverage manufacturing method, and stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed sterilization and then refrigerated and stored Used to prepare the healthy beverage composition of the invention.

Although the composition ratio is a composition suitable for a preferred beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, use purpose.

<3-2> Vegetable juice  Produce

0.5 g of the extract of Example <1-1> was added to 1,000 ml of a vegetable juice such as tomato or carrot to prepare a vegetable juice for health promotion in a conventional manner.

<3-3> Fruit juice  Produce

0.1 g of the extract of Example <1-1> was added to 1,000 ml of fruit juice such as apple or grape, to prepare fruit juice for health promotion in a conventional manner.

Figure 1 is a picture taken by fluorescence microscopy of luminescence caused by deposition on the lateral tibial epiphyseal growth plate after injection of Tetracycline in rats:

     A: saline administration group;

     B: growth hormone (20 µg / kg) administration group; And

     C: mistletoe (HP122, 100 mg / kg) administration group,

Figure 2 is a graph measuring the interval between the growth plate and the luminescence line 48 hours after tetracycline injection in rats:

^* : P <0.05; And

^** : P <0.01,

3 is a graph showing growth rate in rats:

^* : P <0.05; And

^** : P <0.01.

Claims (8)

Pharmaceutical composition for promoting bone growth containing mistletoe extract extracted with water, alcohol or a mixture thereof as a solvent as an active ingredient. The pharmaceutical composition for promoting bone growth according to claim 1, wherein the mistletoe uses a leaf or stem region. The pharmaceutical composition for promoting bone growth according to claim 1, wherein the alcohol is C ₁ to C ₄ lower alcohols. 4. The pharmaceutical composition for promoting bone growth according to claim 3, wherein the lower alcohol is ethanol or methanol. 5. The pharmaceutical composition for promoting bone growth according to claim 4, wherein the ethanol is 70%. The pharmaceutical composition for promoting bone growth according to claim 1, wherein the solvent is extracted by adding 1 to 10 times the weight of mistletoe. The pharmaceutical composition for promoting bone growth according to claim 1, wherein the solvent has an extraction time of 1 to 24 hours. A health food for promoting bone growth, containing mistletoe extract extracted with water, ethanol or a mixture thereof as a solvent as an active ingredient.

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