KR102147101B1 - Composition for protecting neuronal cells comprising Glechoma grandis Kuprian extract - Google Patents

Abstract

The present specification relates to a composition comprising a Geumjeoncho ( Glechoma grandis (A.Gray) Kuprian) as an active ingredient. The composition of the present specification protects nerve cells from oxidative stress and exhibits an effect of inhibiting the death of nerve cells. In addition, it is possible to increase the expression of an antioxidant enzyme that inhibits apoptosis caused by an increase in oxidative stress in cells. Therefore, the composition of the present specification may exhibit an effect of preventing, improving or treating secondary diseases that may occur due to the death of nerve cells from oxidative stress, and thus may be used as a pharmaceutical or food composition.

Description

Composition for protecting neuronal cells comprising Glechoma grandis Kuprian extract}

The present specification relates to a composition comprising a Geumjeoncho extract.

Oxidative stress in nerve cells induces many neurological diseases, especially stroke, amyotrophic lateral sclerosis (Lou Gehrig's disease), Parkinson's disease, and Alzheimer's. Glutamate toxicity induces acute and chronic toxicity in nerve cells. (Andersen, JK et al. Nat. Rev. Neurosci. 2004, 5, S18-S25, Coyle, J. et al. Science 1993, 262, 689-695). Therefore, when the nerve cells are treated with a high concentration of glutamate, the influx of cysteine is suppressed, and glutathione deficiency and active oxygen are increased.

Andersen, J. K. et al. Nat. Rev. Neurosci. 2004, 5, S18-S25, Coyle, J. et al. Science 1993, 262, 689-695

It is an object of the present specification to provide a composition exhibiting an effect of protecting nerve cells from oxidative stress.

In order to achieve the above object, the present invention provides a composition comprising, in one aspect, Glechoma grandis (A.Gray) Kuprian) as an active ingredient.

The composition according to one aspect of the present invention is an antioxidant enzyme that protects nerve cells from oxidative stress, inhibits the death of nerve cells, and inhibits apoptosis caused by an increase in oxidative stress within cells. It has an effect of increasing expression. Therefore, the composition according to an aspect of the present invention can be used as a pharmaceutical or food composition because it can exhibit the effect of preventing, improving, or treating secondary diseases that may occur due to the death of nerve cells from oxidative stress.

1 is a graph showing the cell survival rate of nerve cells when treated with ethanol extract of Geumjeoncho.

In one aspect, the present invention, Glechoma grandis (A.Gray) Kuprian ) It may relate to a composition comprising the extract as an active ingredient.

In one aspect of the present invention, the Geumjeoncho may be a Korean Geumjeoncho.

In one aspect of the present invention, the composition may relate to a composition for protecting nerve cells from oxidative stress or inhibiting nerve cell death.

In one aspect of the present invention, the oxidative stress may be caused by glutamate.

In one aspect of the present invention, the composition may be a composition for treating, preventing or improving diseases caused by the death of nerve cells.

In one aspect of the present invention, the disease caused by the death of nerve cells may be one or more selected from the group consisting of stroke, amyotrophic lateral sclerosis (Lou Gehrig's disease), Parkinson's disease, ischemic stroke, Huntington's disease, and Alzheimer's.

In one aspect of the present invention, the composition may be a composition for treating, preventing or improving neurological diseases caused by oxidative stress, wherein the neurological diseases caused by oxidative stress are stroke, amyotrophic lateral sclerosis (Lou Gehrig's disease ), Parkinson's disease, ischemic stroke, Huntington's disease, and Alzheimer's may be at least one selected from the group.

In one aspect of the present invention, the composition may be a composition for treating, preventing, or improving diseases caused by glutamate toxicity, and diseases caused by glutamate toxicity include stroke, amyotrophic lateral sclerosis (Lou Gehrig's disease), It may be one or more selected from the group consisting of Parkinson's disease, ischemic stroke, Huntington's disease, and Alzheimer's.

In one aspect of the present invention, the composition may be a composition for the treatment, prevention, or improvement of a neurological disease caused by glutamate toxicity, wherein the neurological disease caused by glutamate toxicity may be a neurodegenerative disease, specifically It may be at least one selected from the group consisting of stroke, amyotrophic lateral sclerosis (Lou Gehrig's disease), Parkinson's disease, ischemic stroke, Huntington's disease, and Alzheimer's.

In one aspect of the present invention, the composition may be a composition for promoting the expression of antioxidant enzymes in nerve cells. In one aspect of the present invention, the antioxidant enzyme may inhibit cell death caused by an increase in oxidative stress.

In one aspect of the present invention, the composition may be a composition for reducing or inhibiting active oxygen. In one aspect of the present invention, the composition may be a composition for reducing or inhibiting free radicals generated in cells, specifically nerve cells.

In one aspect of the present invention, the composition may be a pharmaceutical or food composition.

In one aspect of the present invention, the Geumjeoncho extract may be one or more extracts selected from the group consisting of water, organic solvents, and mixtures thereof. Specifically, in one aspect of the present invention, the organic solvent may be one or more selected from the group consisting of C ₁ ~ C ₆ lower alcohol, butylene glycol, and propylene glycol, and more specifically, the lower alcohol may be ethanol. .

In one aspect of the present invention, the Geumjeoncho extract may be a fraction obtained by fractionating the Geumjeoncho organic solvent extract into one or more selected from the group consisting of water, an organic solvent, and a mixture thereof. Specifically, in one aspect of the present invention, the Geumjeoncho extract may be one or more fractions selected from the group consisting of water, ethyl acetate, and mixtures thereof using the ethanol extract of Geumjeoncho.

In one aspect of the present invention, the Geumjeoncho may be at least one selected from the group consisting of leaves, flowers, stems, fruits, roots, and combinations thereof. Specifically, the Geumjeoncho may be one or more selected from the group consisting of leaves, stems, roots, and combinations thereof of Geumjeoncho herbs.

In one aspect of the present invention, the Geumjeoncho extract may have a concentration of 0.1 ug/ml to 2000 ug/ml or 0.1 ug/ml to 1000 ug/ml based on the total volume of the composition. Specifically, in one aspect of the present invention, based on the total volume of the composition, the extract is 0.1 ug/ml or more, 0.5 ug/ml or more, 1 ug/ml or more, 2 ug/ml or more, 3 ug/ml or more , 4 ug/ml or more, 5 ug/ml or more, 5.55 ug/ml or more, 10 ug/ml or more, 15 ug/ml or more, 16 ug/ml or more, 16.5 ug/ml or more, 16.6 ug/ml or more, 16.8 ug/ml or more, 17.0 ug/ml or more, 17.1 ug/ml or more, 17.2 ug/ml or more, 17.5 ug/ml or more, 18 ug/ml or more, 20 ug/ml or more, 50 ug/ml or more, 100 ug/ ml or more, 500 ug/ml or more, or 1000 ug/ml or more and 2000 ug/ml or less, 1000 ug/ml or less, 500 ug/ml or less, 100 ug/ml or less, 50 ug/ml or less, 20 ug/ ml or less, 18 ug/ml or less, 17.5 ug/ml or less, 17.2 ug/ml or less, 17.1 ug/ml or less, 17.0 ug/ml or less, 16.8 ug/ml or less, 16.6 ug/ml or less, 16.5 ug/ml or less , 16 ug/ml or less, 15 ug/ml or less, 10 ug/ml or less, 5.55 ug/ml or less, 5 ug/ml or less, 4 ug/ml or less, 3 ug/ml or less, 2 ug/ml or less, 1 It may be ug/ml or less, 0.5 ug/ml or less, or 0.1 ug/ml or less, but is not limited thereto.

In this specification, "Geumjeoncho ( Glechoma grandis (A.Gray) Kuprian)" refers to plants of the genus Lamiaceae of the dicotyledonous plant of the family Pizzeria. This is cholelithiasis, bladder stones, jaundice, bruises, bronchial asthma, chronic bronchitis, cystitis, edema, and nose. , Mumps, Ongjong, and eczema are known for their efficacy, but no neuronal protective efficacy is known.

In the present specification, "extract" includes all substances obtained by extracting the components of natural substances, regardless of the extraction method, extraction solvent, extracted component, or form of the extract, and after extracting the substance obtained by extracting the component of a natural substance, It is a broad concept including all substances that can be obtained by processing or processing by a method, and specifically, the processing or treatment may be additional fermentation or enzyme treatment of the extract. Therefore, in the present specification, the extract is a broad concept including a fermented product, a concentrate, and a dried product, and specifically, the extract may be a fermented product in the present specification.

In one aspect of the present invention, "Geumjeoncho extract" includes all substances obtained by extracting the components of the extract, regardless of the extraction method, the extraction solvent, the extracted component or the form of the extract, and in the process of extracting the component Contains substances obtained through an extraction method including a process of treating with heat, acid, base, enzyme, etc., and obtained by extracting the substance obtained by extracting the components of the mosquito herb and then processing or processing it by other methods. It is a broad concept that encompasses all possible substances. Specifically, the processing or treatment may be additional fermentation or enzyme treatment of the Geumjeoncho extract. Therefore, the extract of Geumjeoncho of the present specification may be a fermented product.

In one aspect of the present invention, " Glechoma grandis (A.Gray) Kuprian)" may be in the form of an extract, or may be a raw geumjeoncho, a pulverized product of a herbal medicine itself, a dried product of a herbal medicine, a dry pulverized product of a herbal medicine, or a fermented product of a keumjeoncho, but is not limited thereto. There is no restriction on the method of obtaining the kumjeoncho used in the present specification, and may be used after cultivation or commercially available ones, and some or all of the above-ground or root portions of the herb may be used. One or more selected from the group consisting of flowers, stems, fruits, and roots may be used In the case of Kumjeoncho in the present specification, it is not necessarily manufactured through drying and is not limited as long as it is a raw material in a form suitable for extracting the active ingredient of Kumjeoncho .

In one aspect of the present invention, water includes distilled water or purified water, and the organic solvent includes alcohols and acetone, ether, ethyl acetate, diethyl ether, ethyl methyl ketone, and examples of lower alcohols of C ₁ to C ₆ It includes at least one selected from the group consisting of chloroform, but is not limited thereto.

In one aspect of the invention, geumjeoncho extract may comprise a C ₁ ~ C ₆ alcohol extract of geumjeoncho, specifically, the alcohol may be methanol or ethanol.

In one aspect of the present invention, the Geumjeoncho extract may be obtained by a manufacturing method comprising the step of extracting the Geumjeoncho extract with water, an organic solvent, or a mixture thereof.

In one aspect of the present invention, the Geumjeoncho extract may be a crude extract of a solvent selected from the group consisting of water, an organic solvent, and combinations thereof. The organic solvent may be a C ₁ -C ₆ alcohol, and specifically, the C ₁ -C ₆ alcohol may be methanol or ethanol. In one aspect of the present invention, when extracting the Geumjeoncho extract with a solvent, it is preferable to extract by adding a solvent corresponding to about 5 to 15 times (v/w) of the Geumjeoncho extract, and specifically, about 10 times the solvent It is preferable to extract by adding, but is not limited thereto.

In one aspect of the present invention, the extraction may be hot water extraction, ethanol extraction, heat extraction, cold needle extraction, reflux extraction, reflux cooling extraction, or ultrasonic extraction, etc., and there is no limitation as long as the extraction method is obvious to those skilled in the art. The extraction may be hot water extraction or ethanol extraction.

In one aspect of the present invention, extraction may be performed at room temperature, but for more efficient extraction, it may be performed under heated conditions, and specifically, extraction at a temperature of about 40 to 100°C, more specifically about 65 to 75°C However, it is not limited thereto. The extraction time may be performed for 2 hours to 48 hours, specifically 18 to 36 hours, more specifically 20 to 28 hours, and most specifically 22 to 26 hours, but is not limited thereto, and the extraction solvent And conditions such as extraction temperature. The extraction may be extracted once or more several times in order to obtain a larger amount of the active ingredient, specifically 1 to 5 times, more specifically 3 times continuous extraction and a combined extract may be used.

In one aspect of the present invention, the Geumjeoncho extract may include a crude extract of the Geumjeoncho extract as described above, and may also be included as a soluble fraction of an organic solvent obtained by further extracting the crude extract with an organic solvent having a low polarity. In one aspect of the present invention, hexane, methylene chloride, ethyl acetate, n-butanol, and the like may be used as the organic solvent, but is not limited thereto. The extract extracted by the above method or a soluble fraction of the extract may be used as it is, but may be used in the form of an extract after filtration and concentration, and may be used as a dried product after concentration.

In one aspect of the present invention, drying may be evaporation drying, spray drying, or freeze drying, and specifically, freeze drying may be performed at -50 to -70°C for 3 to 4 days during freeze drying.

The pharmaceutical composition according to one aspect of the present invention may be in various oral or parenteral dosage forms. In the case of formulation, it is prepared using diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants that are usually used. Solid preparations for oral administration include tablets, pills, powders, granules, soft or hard capsules, and the like, and these solid preparations include at least one excipient, such as starch, calcium carbonate, and sucrose, in one or more compounds. Or it is prepared by mixing lactose, gelatin, and the like. In addition, in addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral administration include suspensions, liquid solutions, emulsions, and syrups.In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as humectants, sweeteners, fragrances, and preservatives may be included. have. Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilized preparations, and suppositories. As the non-aqueous solvent and the suspension solvent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate may be used. As a base for suppositories, witepsol, macrogol, tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

The pharmaceutical dosage form of the composition according to an aspect of the present invention may be used in the form of a pharmaceutically acceptable salt thereof, and may be used alone or in combination with other pharmaceutically active compounds as well as in a suitable set. The salt is not particularly limited as long as it is pharmaceutically acceptable, for example, hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, hydrofluoric acid, hydrobromic acid, formic acid acetic acid, tartaric acid, lactic acid, citric acid, fumaric acid, maleic acid, succinic acid, methanesulfonic acid. , Benzenesulfonic acid, toluenesulfonic acid, naphthalenesulfonic acid, and the like can be used.

The composition according to an aspect of the present invention can be administered parenterally or orally as desired, and divided into 1 to several times so as to be administered in an amount of 0.1 to 500 mg per 1 kg of body weight per day, specifically 1 to 100 mg. Can be administered. The dose administered to a specific patient may vary according to the patient's weight, age, sex, health status, diet, administration time, administration method, excretion rate, disease severity, and the like.

The pharmaceutical composition according to an aspect of the present invention, respectively, according to a conventional method, oral dosage forms such as powders, granules, tablets, soft or hard capsules, suspensions, emulsions, syrups, drinks, aerosols, ointments, and skin such as creams. It may be formulated and used in any form suitable for pharmaceutical preparations, including external preparations, suppositories, injections, and sterile injectable solutions, and may be specifically formulated and used in the form of injections or skin external preparations.

The composition according to an aspect of the present invention may be administered to mammals such as rats, mice, livestock, humans, etc. by various routes such as parenteral or oral, and all modes of administration can be expected, for example, It can be administered by oral, transdermally, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

The composition according to an aspect of the present invention may be administered by various routes that can be easily applied by a person skilled in the art. In particular, the pharmaceutical composition according to the present specification may be administered as an external preparation for skin by a route applied to the skin surface.

In one aspect of the present invention, the food composition may be a health functional food composition.

The formulation of the food composition according to an aspect of the present invention is not particularly limited, but may be formulated as, for example, a tablet, granule, powder, liquid such as a drink, caramel, gel, bar, or the like. In the food composition of each formulation, in addition to the active ingredients, ingredients commonly used in the field may be appropriately selected and blended by a person skilled in the art according to the formulation or purpose of use without difficulty, and synergistic effects may occur when applied simultaneously with other ingredients.

In the food composition according to an aspect of the present invention, the determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage thereof is, for example, 0.1 mg/kg/day to 5000 mg/kg/day, more Specifically, it may be 50 mg/kg/day to 500 mg/kg/day, but is not limited thereto, and may vary depending on various factors such as age, health condition, and complications of the subject to be administered.

Food compositions according to one aspect of the present invention include, for example, various foods such as chewing gum, caramel products, candies, frozen desserts, and confectionery, beverage products such as soft drinks, mineral water, alcoholic beverages, and health including vitamins or minerals. It may be a functional food.

In addition to the above, the food composition according to an aspect of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and enhancing agents (cheese, chocolate, etc.), pectic acid and salts thereof. , Alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonates used in carbonated beverages, and the like. In addition, functional food compositions according to an aspect of the present invention may include flesh for the manufacture of natural fruit juice and fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The proportion of these additives is not so critical, but is generally included in the range of 0 to about 20 parts by weight per 100 parts by weight of the composition herein.

Hereinafter, the configuration and effects of the present specification will be described in more detail with reference to examples and experimental examples. However, these Examples and Experimental Examples are provided for illustrative purposes only to aid understanding of the present specification, and the scope and scope of the present specification are not limited by the following examples.

[ Example ] Money candle Preparation of extract

After 2,500 g of Geumjeoncho was treated with 2,000 mL of ethanol, stored at room temperature for 3 days and concentrated by filtration to obtain 100 mg of Geumjeoncho extract. The thus obtained ethanol extract of Geumjeoncho was dissolved in a solvent mixture of 40 mL of ethyl acetate and 10 mL of water, and then separated into an organic layer and an aqueous layer. As a result, 30 mg of an ethyl acetate fraction and 20 mg of a water fraction were obtained. Samples of the obtained ethanol extract, ethyl acetate fraction, and water fraction were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mg/mL to prepare a sample.

[ Experimental example ] Oxidative Measurement of cell viability of neurons due to stress

HT-22 cells (Solk Research Institute, USA) derived from hippocampal neurons of mice were added to DMEM (life technology, 11965-092) medium with 10% fetal bovine serum (Gibco) based on the volume of the entire medium. Subculture was performed while changing the medium once every 3 days using a medium to which 1% of antibiotics (penicillin-streptomycin, Gibco) was added. 100 uL of medium per well was added to a 96-well plate, and 5000 HT-22 cells were injected into each well, which was incubated in an incubator under 5% CO ₂ conditions at 37°C for 24 hours, and then dissolved in DMSO. A mg/mL sample (an ethanol extract of the example) was treated at the concentration shown in FIG. After 2 hours of treatment, 5 mM of monosodium glutamate (MSG) was added and cultured in an incubator under 5% CO ₂ conditions at 37° C. for 24 hours, and then cell viability was measured, which is an EZ-Tox reagent (Daeil Lab Service ) Was used to measure the absorbance at 450 nm with a plate reader. As a control, a medium treated with nothing (DMSO in Fig. 1) and a medium treated with only 5 mM monosodium glutamate (MSG in Fig. 1) were set. These results are shown in FIG. 1.

According to the results of FIG. 1, it can be seen that the extract of Geumjeoncho according to an aspect of the present invention exhibits an effect of increasing the survival rate of neurons killed by glutamate. Therefore, the extract of Geumjeoncho according to one aspect of the present invention exhibits the effect of protecting nerve cells from oxidative stress and inhibiting the death of nerve cells, thereby preventing neurological diseases caused by the death of nerve cells or caused by oxidative stress. It could be treated, prevented or improved. In addition, the extract of Geumjeoncho according to an aspect of the present invention will be able to promote the expression of antioxidant enzymes that inhibit apoptosis caused by an increase in oxidative stress in nerve cells.

An example of the formulation of the composition according to an aspect of the present invention is described below, but it can be applied to various other formulations, and this is not intended to limit the present specification, but is intended to be described in detail.

[ Formulation example 1] soft capsule

Ethanol extract 8mg, vitamin E 9mg, vitamin C 9mg, palm oil 2mg, hydrogenated vegetable oil 8mg, sulfur wax 4mg, and lecithin 9mg were mixed and mixed according to a conventional method to prepare a soft capsule filling solution. Each capsule is filled with 400 mg to prepare a soft capsule. In addition to the above, a soft capsule sheet was prepared in a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin, and 10 parts by weight of sorbitol liquid, and the filling solution was filled to prepare a soft capsule containing 400 mg of the composition according to the present specification.

[ Formulation example 2] tablet

Ethanol extract 8mg, vitamin E 9mg, vitamin C 9mg, galactooligosaccharide 200mg, lactose 60mg and maltose 140mg were mixed and granulated using a fluid bed dryer, and then sugar ester 6mg was added. . 500 mg of these compositions are tableted by a conventional method to prepare tablets.

[ Formulation example 3] Drink system

Ethanol extract 8mg, vitamin E 9mg, vitamin C 9mg, glucose 10g, citric acid 0.6g, and liquid oligosaccharide 25g were mixed, and then 300ml of purified water was added and each bottle was filled with 200ml each. After filling the bottle, sterilize at 130°C for 4-5 seconds to prepare a drink.

[ Formulation example 4] granules

Ethanol extract 8mg, vitamin E 9mg, vitamin C 9mg, anhydrous crystalline glucose 250mg and starch 550mg of Example were mixed, molded into granules using a fluid bed granulator, and then filled into a bag to prepare granules.

[ Formulation example 5] Injection

According to the composition shown in Table 1 below, an injection was prepared by a conventional method.

Ingredients content Example of Ethanol Extract of Geumjeoncho 10-50 mg Sterile distilled water for injection Appropriate amount pH adjuster Appropriate amount

[ Formulation example 6] Health functional food

Health functional foods were prepared in a conventional manner according to the composition shown in Table 2 below.

Ingredients content Example of Ethanol Extract of Geumjeoncho 20mg Vitamin A acetate 70μg Vitamin E 1.0mg Vitamin B1 0.13mg Vitamin B2 0.15mg Vitamin B6 0.5mg Vitamin B12 0.2μg Vitamin C 10mg Biotin 10μg Nicotinic acid amide 1.7mg Folic acid 50μg Calcium pantothenate 0.5mg Ferrous sulfate 1.75mg Zinc oxide 0.82mg Magnesium carbonate 25.3mg Potassium Phosphate Monobasic 15mg Dicalcium phosphate 55mg Potassium citrate 90mg Calcium carbonate 100mg Magnesium chloride 24.8mg

The composition ratio of the vitamin and mineral mixture is a mixture of components suitable for a health functional food in a preferred embodiment, but the mixing ratio may be arbitrarily modified.

[ Formulation example 7] healthy drink

A health drink was prepared by a conventional method according to the composition shown in Table 3 below.

Ingredients content Example of Ethanol Extract of Geumjeoncho 1000mg Citric acid 1000mg oligosaccharide 100 g Taurine 1g Purified water Balance

After mixing the above ingredients according to a conventional health drink manufacturing method, stirring and heating at 85° C. for about 1 hour, the resulting solution is filtered and sterilized.

Claims (13)

Contains Geumjeoncho extract as an active ingredient,
The gold candle is a gold candle ( Glechoma grandis (A.Gray) Kuprian),
Stroke, amyotrophic lateral sclerosis (Lou Gehrig's disease), Parkinson's disease, ischemic stroke, Huntington's disease, and in a patient with one or more diseases selected from the group consisting of Alzheimer's, a composition for protecting nerve cells from oxidative stress or inhibiting nerve cell death. The composition of claim 1, wherein the oxidative stress is caused by glutamate. As a composition for the treatment, prevention or improvement of diseases caused by the death of nerve cells,
The composition comprises a Geumjeoncho extract as an active ingredient,
The Geumjeoncho is Geumjeoncho ( Glechoma grandis (A.Gray) Kuprian),
The disease caused by the death of the neuron is at least one selected from the group consisting of stroke, amyotrophic lateral sclerosis (Lou Gehrig's disease), Parkinson's disease, ischemic stroke, Huntington's disease, and Alzheimer's. , Prevention or improvement composition. delete Contains Geumjeoncho extract as an active ingredient,
The gold candle is a gold candle ( Glechoma grandis (A.Gray) Kuprian),
Stroke, amyotrophic lateral sclerosis (Lou Gehrig's disease), Parkinson's disease, ischemic stroke, Huntington's disease, and a composition for promoting the expression of antioxidant enzymes in nerve cells of a patient with at least one disease selected from the group consisting of Alzheimer's. Contains Geumjeoncho extract as an active ingredient,
The gold candle is a gold candle ( Glechoma grandis (A.Gray) Kuprian),
Stroke, amyotrophic lateral sclerosis (Lou Gehrig's disease), Parkinson's disease, ischemic stroke, Huntington's disease and Alzheimer's in patients with one or more diseases selected from the group consisting of a composition for reducing or inhibiting free radicals of neurons. The composition of any one of claims 1 to 3, 5 and 6, wherein the Geumjeoncho is at least one selected from the group consisting of leaves, flowers, stems, fruits, roots, and combinations thereof of the Keumjeoncho herb. The composition of claim 7, wherein the Geumjeoncho is at least one selected from the group consisting of leaves, stems, roots, and combinations thereof. The composition according to any one of claims 1 to 3, 5 and 6, wherein the extract of Geumjeoncho is one or more extracts selected from the group consisting of water, organic solvents, and mixtures thereof. The composition of claim 9, wherein the organic solvent is at least one selected from the group consisting of C ₁ to C ₆ lower alcohol, butylene glycol, and propylene glycol. 11. The composition of claim 10, wherein the lower alcohol is ethanol. The composition according to any one of claims 1 to 3, 5, and 6, wherein the extract of Kumpanzee is a concentration of 0.1 ug/ml to 1000 ug/ml based on the total volume of the composition. The composition according to any one of claims 1 to 3, 5 and 6, wherein the composition is a pharmaceutical or food composition.

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