KR20080065667A - Lactic acid bacterium having immunoregulatory activity derived from moromi for wine fermentation - Google Patents

Abstract

The invention relates to a bacterium belonging to the genus Lactobacillus having an immunoregulatory activity, a composition containing the same, and a method of efficiently isolating a lactic acid bacterium having an immunoregulatory activity. The bacterium belonging to the genus Lactobacillus of the invention has an immunoregulatory activity, and if the composition of the invention is taken or the like, a decrease in immune function can be suppressed by activating the immune function. Further, according to the invention, a lactic acid bacterium having an immunoregulatory activity can be efficiently obtained.

Description

LACTIC ACID BACTERIUM HAVING IMMUNOREGULATORY ACTIVITY DERIVED FROM MOROMI FOR WINE FERMENTATION

This invention relates to the Lactobacillus genus which has an immunomodulatory effect, the composition containing it, and the isolation | separation method with good efficiency of the lactic acid bacterium which has an immunomodulatory effect.

Lactic acid bacteria are known to have beneficial physiological activities such as intestinal action and immunostimulating action. Moreover, the recognition as a material with health efficacy for a general consumer is also high. Most of the lactic acid bacteria having such beneficial physiological activity are lactic acid bacteria derived from animal material separated from fermented dairy products or intestinal tract.

On the other hand, the strain which has an immune activating effect is discovered also in the vegetable lactic acid bacteria which uses a plant material as a separation source. For example, Japanese Laid-Open Patent Publication No. 10-167972 (published: June 23, 1998) discloses Lactobacillus plantarum L-137 strain as a lactic acid bacterium having an immunostimulating action. . In addition, Lactobacillus brevis Labre strain isolated from Kyoto's traditional pickles Suguki, and Lactobacillus pentosus DA74N strain isolated from Shibatsuke also have immunosuppressive action. (Reference: (1) Japanese Patent Publication No. 7-55908 (published: July 26, 1994), (2) Pasken Journal 15. 21-26, 2002, Kishi Atsuko, Kokubo Aoi, Akatani Kaoru, Eriko Ogiya, Tetsuya Fujita, Mota Kishida: Screening for Selection of Useful Lactic Acid Bacteria 1) In Vitro Immune Revitalization Effect of Vegetable Lactic Acid Bacteria in Human Peripheral Blood, (3) 6th Intestine Bacterial Society (2002.05.30-31) Yoji, Akatani Kaoru, Kishi Atsuko, Eriko Ogiya, Kokubo Aoi, Fujita Tetsuya, and Kishida Motaro).

Vegetable lactic acid bacteria which use plant material as a separation source are known to be deeply involved in pickling vegetables and brewing of alcoholic beverages, and many bacteria can be separated from the brewing process. In addition to the above, it is considered that there are strains having useful physiological activities such as immunostimulating action. For example, as a strain isolate | separated from the wine brewing process of the liquor brewing process, the mainly Lactobacillus plantarum with high isolation | separation frequency is mentioned. However, for the bacteria isolated from the liquor brewing process of such liquor, research on contribution to the action, flavor, etc. in the brewing is important, and the effect of the bacteria on the living body is not studied very much. Therefore, it can be expected to find strains with useful physiological activity among the lactic acid bacteria isolated from the mainstream brewing process. In addition, when the lactic acid bacteria are used, a unique fermentation odor may be generated, and depending on the type of food to be produced, there may be a need for measures and considerations for the sensory function.

Disclosure of the Invention

It is an object of the present invention to provide a method for separating lactic acid bacteria having an immunomodulatory action, a composition containing the same, and a lactic acid bacteria having an immunomodulatory action in view of the present situation.

MEANS TO SOLVE THE PROBLEM In order to solve such a subject, the present inventors investigated the immunomodulatory action with respect to the lactic acid bacteria isolate | separated from the product in a liquor brewing process, and to the lactic acid bacteria which belong to the selected Lactobacillus plantarum and Lactobacillus brevis among them. In this regard, the immunomodulatory action was examined in more detail. As a result, the lactic acid bacterium had a high immunomodulatory effect, and it was found that the fermented product using the same exhibited good flavor, thus completing the present invention.

Specifically, the lactic acid bacteria isolated from the product in the mainstream brewing process were compared with the induction ability of Th1 cytokine to interleukin 12 (hereinafter abbreviated as "IL-12"). It has been found that lactic acid bacteria can be isolated, and in particular, strains belonging to Lactobacillus plantarum and Lactobacillus brevis increase the production of IL-12 when treated in vitro to celiac macrophages prepared from mice. I found out. In addition, it was also confirmed that heating bacterial solution was administered in vivo to the mouse intraperitoneal to induce production of IL-12 of Th1-type cytokine.

That is, the present invention is the genus Lactobacillus which has an immunomodulatory effect contained in the microorganism-containing component of the liquor production process. In particular, the microbial-containing component is fermented moromi, and the liquor manufacturing process is a wine brewing process.

Moreover, this invention is Lactobacillus plantarum or Lactobacillus brevis which has an immunomodulatory effect.

In the present invention, Lactobacillus plantarum having an immunomodulatory action is Lactobacillus plantarum SAM2446 strain (FERM ABP-10438) or Lactobacillus brevis Lactobacillus brevis SAM2447 strain (FERM ABP-10439) .

Moreover, this invention is a composition which has an immunomodulatory effect containing the Lactobacillus genus which has an immunomodulatory effect contained in the microorganism containing component of a liquor manufacturing process. In particular, the composition is a food or beverage or a medicine.

In addition, the present invention includes a step of separating the microorganisms from the microorganism-containing component of the liquor manufacturing process, the step of measuring the immunomodulatory action of each separated microorganism, and the step of selecting a lactic acid bacterium with a strong immunomodulatory action, immunomodulatory action It is a method of separating the lactic acid bacteria having. In particular, the microbial-containing component is fermented moromi, and the liquor manufacturing process is a wine brewing process.

1 is a graph showing the results of measurement of serum IL-12 concentration after intraperitoneal administration of 12 kinds of lactic acid bacteria to mice.

2 shows the 16S rRNA gene sequence of the translated SAM2446 strain. The underlined portion shows a portion different from the arrangement with Lactobacillus plantarum JCM1149T (ATCC14917).

3 shows the 16S rRNA gene sequence of the translated SAM2447 strain. The underlined portion represents a portion different from the arrangement with Lactobacillus brevis JCM1059T (ATCC14869).

4 shows measurement results of NK activity.

5 shows measurement results of IFN-γ concentration.

Implement the invention  Best form for

<Lactobacillus genus>

The Lactobacillus genus is a kind of lactic acid bacteria, and the present invention is a Lactobacillus genus which has an immunomodulatory effect contained in the microorganism-containing component of the liquor production process. As for Lactobacillus genus, Lactobacillus plantarum or Lactobacillus genus belonging to Lactobacillus brevis is preferable.

The bacteriological properties of the genus Lactobacillus are BERGEY'S MANUAL of Systematic Bacteriology (Vol. 1, 1984, Vol. 2, 1986, Vol. 1989, Vol. 4, 1989).

As a typical example of the lactic acid bacteria which concerns on this invention, Lactobacillus plantarum SAM2446 strain and Lactobacillus brevis SAM2447 strain are mentioned. This bacterium has been deposited in an independent administrative corporation, the Industrial Technology Research Institute, and the Patent Biological Deposit Center (1 Chuo Dai 6, Higashi 1-chome, Tsukuba-shi, Ibaraki, Japan) .The accession numbers are FERM ABP-10438 and FERM ABP-10439. to be. The microbiological properties of the Lactobacillus plantarum SAM2446 strain (FERM ABP-10438) are shown in Table 1, and the microbial properties of the Lactobacillus Brevis SAM2447 strain (FERM ABP-10439) are shown in Table 2.

TABLE 1

Lactobacillus Planta Room SAM2446 Note (FERM ABP-10438)

Cell Form Spore Gram Staining Motile Apocatalase Reaction Growth at 15 ° C Magnetization of Growth Sugar at 45 ° C (positive: +, negative:-, weakly positive: w) Glycerol D-Arabinose L-Arabinose Ribose D- Xylose L-Xylose Galactose Glucose Fructose Mannose Rhamnose Mannitol Sorbitol α-Methyl-D-Mannoside α-Methyl-D-Glucoside Cellobiose Lactose Trehalose Raffinose Xylitol Bacillus not formed Positive None None Negative Good Not developed--+ +--+ + + +-+ +--+ +----

TABLE 2

Lactobacillus Brevis SAM2447 Note (FERM ABP-10439)

Cell Form Spore Gram Staining Motile Apocatalase Reaction Growth at 15 ° C Magnetization of Growth Sugar at 45 ° C (positive: +, negative:-, weakly positive: w) Glycerol D-Arabinose L-Arabinose Ribose D- Xylose L-Xylose Galactose Glucose Fructose Mannose Rhamnose Mannitol Sorbitol α-Methyl-D-Mannoside α-Methyl-D-Glucoside Cellobiose Lactose Trehalose Raffinose Xylitol Bacillus not formed Positive None None Negative Good Not developed-w + +--+ + +--w--w------

In the present invention, the term "having an immunomodulatory action" refers to an action of activating this when a steady state or immune function is lowered (immune reactivation action) or to suppressing an appropriate immune function when the immune function is excessively advanced. It means having the action of at least one of the effect of making a state (immunosuppressive action) and the effect of optimizing the balance between cellular immunity and humoral immunity. Examples of immunomodulatory actions include promoting or inhibiting cytokine production, activating lymphocytes, enhancing NK (natural killer) activity, improving Th1 / Th2 balance, suppressing immune lowering, antiallergic action, and the like. Although it may be mentioned, It is not limited to these.

The Lactobacillus genus of the present invention can be obtained by separating microorganisms from the microorganism-containing component of the liquor production process, measuring the immunomodulatory action of each microorganism separated, and selecting a bacterium with a strong immunomodulatory action.

In the present invention, the "liquor production process" is a series of processes related to alcohol production. Here, the "liquor" includes fruit wines such as wine, malt fermented beverages such as beer and sparkling wine, distilled wines such as sake, whiskey and the like.

The microorganism-containing component refers to a component in which microorganisms exist in the liquor production process, and is, for example, an injection raw material or fermented urine.

<Composition>

The Lactobacillus genus according to the present invention can be used as a composition containing the Lactobacillus genus. In particular, use as a pharmaceutical composition having an immunomodulatory action is preferable.

As Lactobacillus genus contained in the said composition, Lactobacillus genus (live and dead bacteria), Lactobacillus genus containing material, Lactobacillus genus fermentation product, Lactobacillus genus processed material, etc. are mentioned. Live bacteria can be obtained from Lactobacillus bacteria-containing substances such as the Lactobacillus bacteria culture medium. The dead bacteria can be obtained by, for example, heating, ultraviolet irradiation, formalin treatment or the like to live bacteria. The obtained live bacteria and dead bacteria can be further treated by grinding or crushing.

That is, the Lactobacillus genus containing composition using the Lactobacillus genus of this invention is at least any one of the Lactobacillus genus which concerns on this invention, the said Lactobacillus genus containing substance, the said Lactobacillus genus fermentation product, and the said Lactobacillus genus processed material. What is necessary is just to contain. Examples of the Lactobacillus genus-containing material include, for example, Lactobacillus genus suspensions, Lactobacillus genus cultures (including microorganisms, culture supernatants, and media components), and Lactobacillus genus culture medium (solids removed from the bacterial cultures). And Lactobacillus genus fermented milk (Lactobacillus genus beverage, acid oil, yoghurt, etc.) are mentioned. As a medium used for culturing microorganisms belonging to the genus Lactobacillus, any medium that can grow microorganisms belonging to the genus Lactobacillus can be used without particular limitation. As the Lactobacillus fungus treated material, for example, ground products, crushed products, liquid products (extracts, etc.), concentrates, pastes, dried products (spray dried products, freeze-dried products, vacuum dried products, drum dried products, etc.), dilutions Etc. can be mentioned. In addition, since the Lactobacillus plantarum SAM2446 strain and the Lactobacillus brevis SAM2447 strain which are related to this invention are isolate | separated from the wine brewing process, for example, the fermentation which fermented vegetables and cereals by the Lactobacillus genus bacterium which concerns on this invention was carried out. It is also preferable to contain water as one form of the lactic acid bacteria containing composition using the Lactobacillus genus of this invention. Moreover, as mentioned above, since the Lactobacillus genus concerning this invention was isolate | separated from the original foodstuff, safety is high.

It is preferable to use the Lactobacillus genus containing composition using the Lactobacillus genus of this invention as a pharmaceutical composition which has an immunomodulatory effect. For example, food and drink, medicine, feed, etc. are mentioned. When used for food and drink, it is preferable to carry out as a health food which has an immunomodulatory effect. Moreover, what is necessary is just to mix with various components, such as a well-known sweetener, an acidulant, and a vitamin, and to make it the product suited to a user's preference. For example, it can be provided in the form of dairy products such as tablets, capsules, drinks, troches, gums, yogurts, ice creams, beverages, alcoholic beverages, seasonings, processed foods, desserts, confectionery.

Moreover, when used for pharmaceuticals, it is formulated using the well-known adjuvant normally used in the art of manufacture of medicines, such as excipient, binder, disintegrant, glidant, copper, dissolution aid, suspending agent, coating agent, etc. in a main medicine. can do. Examples of the formulation include tablets, capsules, granules, powders, syrups, suppositories, injections, and the like, and are not particularly limited. Examples of the route of administration of the drug include oral administration, rectal administration, and enteral administration, but are not particularly limited.

When used for feed, the cells can be mixed with raw materials such as pet food or livestock feed. Or you may add to the commercial animal feed. Especially, it can use suitably for diet, such as a dog, a cat, or a bird of a chicken.

<Isolation method of lactic acid bacteria having immunomodulatory action>

The measurement of an immunomodulatory effect may be any of methods known to those skilled in the art. For example, in the interleukin 12 (hereinafter abbreviated as "IL-12") induction test of the Th1 cytokine, by measuring the amount of IL-12 produced by in vitro treatment of peritoneal macrophages prepared from mice, Conduct.

Selection of lactic acid bacteria having a strong immunomodulatory effect can be performed by selecting strains having a high immunomodulatory effect by measuring the immunomodulatory effect. What is necessary is just to set suitably as a selection criteria, for example, the comparison with a lactic acid bacterium for which a known immunomodulatory effect is known, a comparison with a control, or a mean value. In addition to the immunomodulatory action, it is possible to add to the selection criteria whether or not good proliferation is exhibited in the medium for lactic acid bacteria.

In the present invention, the "liquor production process" is a series of processes related to alcohol production. Here, the "liquor" includes fruit wines such as wine, malt fermentation drinks such as beer and sparkling wine, distilled wines such as sake whiskey, and the like.

The microorganism-containing component refers to a component in which microorganisms exist in the liquor production process, and is, for example, an injection raw material or fermented urine.

As a separation source, by using a fermented moromi or the like in the wine brewing step, lactic acid bacteria having an immunomodulatory effect can be separated very efficiently.

EXAMPLES The present invention will be described in more detail with reference to Examples, but the present invention is not limited to the above-described embodiments, and various modifications are possible within the scope shown in the claims, and combinations of the technical means disclosed in the different embodiments are appropriately combined. Embodiment obtained by making is also included in the technical scope of this invention.

Example 1

[Use Lactobacillus]

From wine brewing, lactic acid bacteria were isolated for 104 weeks and used in experiments comparing interleukin 12 (IL-12) inducing ability to select strains having high Th1 type immune activating ability.

[Induction of IL-12 by In Vitro Stimulation]

Intraperitoneal administration of 4.05% thioglycolate to BALB / c mice (7 weeks old male), 4 days later, intraperitoneal macrophages were recovered using PBS, and 2 × using RPMI medium containing 10% FBS. After adjusting to 10 ⁶ cells / ml, it was sprinkled 0.5 ml in a 24 well plate. Heat killing bacteria (1 µg / ml) of each strain was added to each well, and IL-12 concentration in the culture supernatant after 24 hours of culture was measured. The control was supposed to be dead bacteria free. IL-12 (p70) was measured because the active form of IL-12 is p70 to which p35 and p40 subunits are bound. In addition, the OptEIA mouse IL-12 measurement kit (made by BD Pharmingen) was used for the measurement of IL-12.

As a result of evaluating IL-12 acid production by in vitro for the 104 strains of isolated lactic acid bacteria, many strains having IL-12 acid production were included. For some of these, IL-12 production was thought to have a sufficiently high activity compared with lactic acid bacteria whose immunomodulatory activity is known. Of these, for 12 weeks having a IL-12 production capacity more than 10 times the control, it was selected as a week having a high IL-12 production capacity.

Considering a very high criterion of 10 times for the control, this probability was considered to be a fairly high value, and wine brewing was considered to be very useful as a source of immunoregulatory lactic acid bacteria.

TABLE 3

Example 2

[Use Lactobacillus]

Twelve weeks of lactic acid bacteria selected in Example 1 were used for the experiment comparing IL-12 inducing ability by in vivo stimulation.

[Induction of IL-12 by In vivo Stimulation]

BALB / c mice (7 weeks, males) were administered intraperitoneally with a suspension (solvent: physiological saline) of heated bacteria (500 µg / 0.2 ml / mouse) of each strain, and after 6 hours, after cervical dislocation, Blood was collected. In addition, the control mice were dosed with an equal amount of physiological saline. After blood collection, serum was collected by centrifugation, and IL-12 concentration in serum was measured using an OptEIA mouse IL-12 measurement kit (manufactured by BD Pharmingen).

The results are shown in FIG. Serum IL-12 concentration was especially strong in A and L weeks. Thus, they were named as SAM2446 and SAM2447, respectively.

Example 3 Identification of SAM2446 strain and SAM2447 strain

The results of the examination of the bacteriological properties of SAM2446 strains are shown in Table 1, and the results of the examination of the bacteriological properties of SAM2447 strains are shown in Table 2.

Based on the results of these studies, the SAM2446 strain was estimated to be Lactobacillus plantarum and the SAM2447 strain was lactobacillus brevis. Lactobacillus plantarum is known both with and without magnetization of sorbitol. As shown in Table 1, SAM2446 strain is a lactic acid bacterium having magnetization of sorbitol. Incidentally, both Lactobacillus and Brevis are known to have D-xylose magnetization and non-magnetic properties. As shown in Table 2, the SAM2447 strain is a lactic acid species that does not have D-xylose magnetization.

DNA was extracted from the SAM2446 and SAM2447 strains, followed by translation of the 16S rRNA gene region. The 16S rRNA gene sequence (SEQ ID NO: 1) of the decoded SAM2446 strain was identified as Lactobacillus plantarum because 99% homology with the 16S rRNA gene sequence of Lactobacillus plantarum JCM1149T (ATCC14917) was obtained ( 2). The 16S rRNA gene sequence of the SAM2447 strain (SEQ ID NO: 2) was identified as Lactobacillus brevis because 97% homology with the 16S rRNA gene sequence of Lactobacillus Brevis JCM1059T (ATCC14869) was obtained (Fig. 3).

Example 4 Immune Revitalization and Immune Regulation by Oral Ingestion

C57BL / 6 mice (Nihon Charles River Co., Ltd., 7 weeks male and 4 males, 1 group) were freely ingested with SAM2446 strain and SAM2447 strain (killing bodies) for 1 week in negative water (equivalent to 0.2 mg / day). Moreover, the control group which does not ingest SAM2446 week and SAM2447 week was formed. After one week the spleens were removed. Splenocytes were prepared from the spleens removed according to a conventional method, and NK activity and cytokine acid production were measured. NK activity was co-cultured with spleen cells and target cells Yac-1 at a ratio of 200: 1 or 100: 1 for 4 hours, and cytotoxic activity of splenocytes was measured by PINK method using flow cytometry. In addition, 2.5 mg / ml of concanavalin A (Nakaraitesk) was allowed to act on spleen cells (5 × 10 ⁶ cells / ml) for 24 hours, and IFN-γ concentration produced in the culture supernatant was determined by ELISA method (OptEIA (BD BioScience). Was measured).

The measurement result of NK activity is shown in FIG. In FIG. 4, NK activity (%) shows cytotoxicity with respect to Yac-1 of mouse spleen cells, and E: T ratio shows the spleen cell number: Yac-1 number which reacted. As is apparent from FIG. 4, the NK activity of spleen cells prepared from mice orally ingested with SAM2446 and SAM2447 strains was significantly increased as compared with the control.

The measurement result of IFN- (gamma) concentration is shown in FIG. As is apparent from FIG. 5, the spleen cells prepared from mice orally ingested with SAM2446 and SAM2447 were significantly increased in IFN-γ concentrations as compared with the control.

As mentioned above, NK activity increased and the production | generation of IFN- (gamma) which is Th1-type cytokine was accelerated | stimulated in the mouse which orally ingested SAM2446 week and SAM2447 week. Therefore, it became clear that the SAM2446 strain and the SAM2447 strain have an immunostimulating action and an immunomodulatory action.

Example 5 Preparation of Soymilk Fermentation

The SAM2446 main fermentation bacterial solution was added to commercially available soymilk so as not to be subjected to other microbial contamination, and left standing at 30 ° C for 24 hours to obtain a soymilk fermentation product. The fermentation proceeded rapidly, and the acidity rise and pH fall were seen. The obtained fermented product did not exhibit an unpleasant fermentation odor or the like compared with the conventional plant fermented product, and exhibited a sensory excellent flavor.

Example 6 Preparation of Soymilk Added with Lactic Acid Bacteria Powder

After heat sterilization of the SAM2447 main fermentation bacteria solution, the cells were collected by centrifugation (3000 rpm, 10 minutes), and the obtained cells were freeze-dried. The lactic acid bacteria powder-added soy milk obtained by adding and stirring the lactic acid bacteria cell powder to commercially available soymilk and suspended in soymilk exhibited a taste that was superior to conventional lactic acid bacteria-containing fermented foods without any unpleasant fermentation odor. .

Example 7 Preparation of Lactic Acid Bacteria-Containing Composition

Preparation Example 1 Drug Containing Lactic Acid Bacteria

refine :

By the method shown below, the pharmaceutical product (tablet) containing a lactic acid bacterium was manufactured.

50 g of SAM2446 main cell powder was mixed with 250 g of lactose and 2 g of magnesium stearate, and compressed into a single tableting machine to prepare a tablet having a diameter of 10 mm and a weight of 300 mg.

Granules:

0.5 g of magnesium stearate was added to 100 g of SAM2447 main cell powder, which was compacted, pulverized, sieved, and sieved to obtain a granule of 20 to 50 mesh.

Preparation Example 2 Various Foods Containing Lactic Acid Bacteria

Various food-drinks containing the lactic acid bacterium were manufactured with the composition shown below.

TABLE 4

Fucked:

(Composition) (parts by weight)

Powder Sorbitol 99.7

Spices 0.2

SAM2446 Main Cell Powder 0.05

Sorbitol seed             0.05

Total amount 100.00

TABLE 5

Jelly (coffee jelly):

(Composition) (parts by weight)

15.0 per Granyu

Gelatin 1.0

Coffee Extract 5.0

Water 78.93

SAM2447 Main cell  Powder 0.07

Total amount 100.00

TABLE 6

juice :

(Composition) (parts by weight)

Frozen Concentrated Wenzhou Citrus Juicer 5.0

Fructose glucose liquid 11.0

Citric Acid 0.2

L-ascorbic acid 0.02

SAM2446 Main Cell Powder 0.05

Spices 0.2

Pigment 0.1

Water 83.43

Total amount 100.00

TABLE 7

carbonated drink :

(Composition) (parts by weight)

8.0 per granue

Concentrated Lemon Juicer 1.0

L-ascorbic acid 0.10

Citric Acid 0.09

Sodium Citrate 0.05

Colorant 0.05

Spices 0.15

Sparkling water 90.55

SAN12447 Main cell  Powder 0.01

Total amount 100.00

TABLE 8

Lactic acid bacteria drink:

(Composition) (parts by weight)

Milk Solid 21% Fermented Milk 14.76

Fructose glucose liquid sugar 13.31

Pectin 0.5

Citric Acid 0.08

Spices 0.15

Water 71.14

SAM2446 Main cell  Powder 0.06

Total amount 100.00

TABLE 9

Lactic acid bacteria drink:

(Composition) (parts by weight)

SAM2447 main fermented milk with 21% milk solids 14.82

Fructose glucose liquid sugar 13.31

Pectin 0.5

Citric Acid 0.08

Spices 0.15

Water 71.14

Total amount 100.00

TABLE 10

Coffee Drinks:

(Composition) (parts by weight)

8.0 per granue

Skim milk powder 5.0

Caramel 0.2

Coffee Extract 2.0

Spices 0.1

Polyglycerin Fatty Acid Ester 0.05

Saline 0.05

Water 84.56

SAM2446 Main cell  Powder 0.04

Total amount 100.00

TABLE 11

Alcoholic drinks:

(Composition) (parts by weight)

50% by volume ethanol 32.0

Sugar 8.4

Juicer 2.4

SAM2447 Main Cell Powder 0.2

Purified water                             57.0

Total amount 100.0

TABLE 12

Sparkling wine:

(Composition) (parts by weight)

Sparkling wine 99.98

SAM2447 Main cell  Powder 0.02

Total amount 100.0

Preparation Example 3 Feed containing Lactobacillus

The feed containing lactic acid bacteria was manufactured with the composition shown below.

TABLE 13

feed :

(Composition) (parts by weight)

Commercial Dock Food 99.98

SAM2446 Main cell  Powder 0.02

Total amount 100.0

When the Lactobacillus genus bacterium of the present invention is ingested, the immune function can be suppressed by activating the immune function. In addition, by adjusting the balance of the immune function, excessive hyperactivity of the immune function adversely affecting the living body and various unpleasant symptoms in the living body derived therefrom can be suppressed. Moreover, according to this invention, the lactic acid bacterium which has an immune regulation effect can be obtained efficiently.

                         SEQUENCE LISTING <110> Suntory Limited <120> A novel Lactobacillus bacterium having immunomodulationg effects and a composition for immunomodulation containing it <130> YCT-1198 <160> 4 <170> PatentIn version 3.1 <210> 1 <211> 242 <212> DNA <213> Lactobacillus plantarum <400> 1 atacatgcaa gtcgaacgaa ctctggtatt gattggtgct tgcatcatga tttacatttg 60 agtgagtggc gaactggtga gtaacacgtg ggaaacctgc ccagaagcgg gggataacac 120 ctggaaacag atgctaatac cgcataacaa cttggaccgc atggtccgag tttgaaagat 180 ggcttcggct atcacttttg gatggtcccg cggcgtatta gctagatggt ggggtaacgg 240 ct 242 <210> 2 <211> 353 <212> DNA <213> Lactobacillus plantarum <400> 2 aggcggctgg ttcctaaaag gttaccccac cgactttggg tgttacaaac tctcatggtg 60 tgacgggcgg tgtgtacaag gcccgggaac gtattcaccg cggcatgctg atccgcgatt 120 actagcgatt ccgacttcat gtaggcgagt tgcagcctac aatccgaact gagaatggct 180 ttaagagatt agcttactct cgcgagttcg caactcgttg taccatccat tgtagcacgt 240 gtgtagccca ggtcataagg ggcatgatga tttgacgtca tccccacctt cctccggttt 300 gtcaccggca gtctcaccac aatgcccaac ttaatgctgg caacagataa taa 353 <210> 3 <211> 346 <212> DNA <213> Lactobacillus brevis <400> 3 aacgctggcg gcatgcctaa tacatgcaag tcgaacgagc ttccgttgaa tgacgtgctt 60 gcactgattt taacaatgaa gcgagtggcg aactggtgag taacacgtgg gaaatctgcc 120 cagaagcagg ggataacact tggaaacagg tgctaatacc gtataacaac aaaatccgca 180 tggattttgt ttgaaaggtg gcttcggcta tcacttctgg atgatcccgc ggcgtattag 240 ttagttggtg aggtaaaggc ccaccaagac catgatgcgt agccgacctg agagggtaat 300 cgcccacatt gggactggca cacggcccat actcctacgg gaggca 346 <210> 4 <211> 524 <212> DNA <213> Lactobacillus brevis <400> 4 ctaatcatct gtcccacctt agacggctga ctcccgaagg ttatctcacc ggctttgggt 60 gttacaaact ctcatggtgt gacgggcggt gtgtacaagg cccgggaacg tattcaccgc 120 ggcatgctga tccgcgatta ctagcgattc caacttcatg taggcgagtt gcagcctaca 180 atccgaactg agaacggctt taagagatta gcttagcctc acgacttcgc gactcgttgt 240 accgtccatt gtagcacgtg tgtagcccag gtcataaggg gcatgatgat ttgacgtcat 300 ccccaccttc ctccggtttg tcaccggcag tctcaccaga gtgcccaact gaatgctggc 360 aactgataat aagggttgcg ctcgttgcgg gacttaaccc aacatctcac gacacgagct 420 gacgacaacc atgcaccacc tgtcattctg tccccgaagg gaacgtctta tctctaagat 480 tggcggaaga tgtcaagacc tggtaaggtt cattcgcgta gctt 524  

Claims (15)

The Lactobacillus genus which has an immunomodulatory effect contained in the fermented moromi of a wine brewing process. The method of claim 1, The bacterium Lactobacillus is Lactobacillus plantarum. The method of claim 1, The bacterium Lactobacillus brevis is a genus of Lactobacillus. The method of claim 2, A bacterium in which Lactobacillus plantarum has sorbitol magnetization. The method of claim 3, wherein A bacterium in which Lactobacillus brevis does not have magnetization of D-xylose. The method of claim 2, Lactobacillus plantarum is Lactobacillus plantarum SAM2446 strain (FERM ABP-10438). The method of claim 3, wherein The bacterium Lactobacillus brevis is Lactobacillus brevis SAM2447 strain (FERM ABP-10439). The composition containing the Lactobacillus genus of any one of Claims 1-7, and having an immunomodulatory effect. The method of claim 8, The composition is a food-drinks. The method of claim 8, The composition is a pharmaceutical. The method of claim 8, The composition is a feed. As a method of separating lactic acid bacteria having an immunomodulatory action, (a) separating the microorganism from the microorganism-containing component of the liquor manufacturing process, (b) measuring the immunomodulatory activity of each isolated microorganism, and (c) A process for selecting lactic acid bacteria with strong immunomodulatory action The method comprising a. The method of claim 12, Lactic acid bacteria is Lactobacillus genus. The method according to claim 12 or 13, The microorganism-containing component is fermented moromi. The method according to any one of claims 12 to 14, Wherein said liquor making process is a wine brewing process.

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