KR102567790B1 - Lactobacillus gasseri GFC_1220(GFC_EJR_JOY) for alcohol metabolism enzyme activity and food composition comprising the same as an effective ingredient - Google Patents

Abstract

The present invention relates to a Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain having an alcohol metabolizing enzyme activity and a food composition containing the same as an active ingredient.
The novel Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain provided by the present invention is derived from breast milk, and has an aldehyde dehydrogenase, so it has an excellent acetaldehyde decomposition effect and is resistant to alcohol. Since it is excellent in protecting liver cells from toxicity by poisoning, it can be used as a food composition for relieving hangover and improving alcoholic liver damage.

Description

Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain having alcohol metabolism enzyme activity and a food composition comprising it as an active ingredient

The present invention relates to a Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain (accession number: KCTC 13840BP) having an excellent alcohol metabolizing enzyme activity and a hangover relief effect, and a food composition containing the same as an active ingredient .

A hangover refers to a phenomenon that occurs when a person wakes up from sleep after drinking alcohol, such as unusual discomfort, headache, and reduced mental and physical performance. These phenomena are intermediate metabolites produced in the process of breaking down alcohol in the liver It is caused by toxicity caused by phosphorus acetaldehyde.

The liver is a very important organ that manages energy metabolism in our body as a whole, has the function of storing or distributing various metabolites and compounds throughout the body, detoxifying toxins, or playing the role of a major immune organ.

Since the alcohol cannot be stored in the body and must be excreted from the body, the alcohol metabolism process in the liver is very important. It is oxidized to acetaldehyde by aldehyde dehydrogenase, oxidized to acetic acid by aldehyde dehydrogenase, and finally excreted from the lungs through the process of decomposing acetic acid into water and carbon dioxide.

When alcohol enters the body, about 20% is absorbed in the stomach, and most of the rest is absorbed in the small intestine, and 80-90% of the alcohol not absorbed into the body is metabolized in the liver. It is decomposed, and the acetaldehyde is toxic and damages liver cells. Continuous and excessive drinking causes acute/chronic alcoholic liver disease accompanied by liver cell damage.

These alcoholic liver diseases are largely classified into alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis. The mechanisms of liver damage include alcohol itself, metabolites such as acetaldehyde, and immune responses. In particular, acetaldehyde It acts as the main culprit of liver toxicity, such as peroxidation of fatty liver, binding with cytoplasm, disturbance of mitochondrial electron transport system, function disruption of microtubls, formation of proteins and binding substances, and increase in collagen synthesis. If a large portion of alcohol metabolism by the liver can be processed in the small or large intestine, the burden on the liver can be reduced, and furthermore, liver damage can be suppressed.

On the other hand, most of the lactic acid bacteria are Gram-positive bacteria, and the cell wall exists outside the cell membrane, and the cell wall forms a complex layered structure of peptidoglycan, lipoprotein, and teichoic acid. These cell wall components combine with MAMPs by PRR (Pattern Recognition Receptor) expressed in the human intestinal mucosa to trigger an immune response. In addition, it is known to inhibit harmful microorganisms in the intestines and is metabolized in the intestines to have physiological activities such as enhancement of intestinal peristalsis, regulation of the immune system, and anti-inflammation. There are reports showing a photoprotective effect.

Probiotics, which have recently been in the limelight as beneficial bacteria in the intestine, are widely applied to the prevention and treatment of diseases, and in particular, are widely known to have an effect of inhibiting the growth and decay activities of intestinal putrefactive bacteria and pathogens. It has also been reported that probiotics suppress the growth of harmful bacteria that produce ammonia or amines, thereby suppressing the production of toxic substances, thereby reducing the amount of ammonia and alpha amino nitrogen in the blood, thereby improving the symptoms of liver disease patients. In addition, among these useful bacteria, it has been reported that it protects the liver from alcohol and improves specific functions of the liver. Recently, a study on the possibility of rapidly decomposing alcohol by actively promoting alcohol metabolism using useful lactic acid bacteria such as probiotics Lactobacillus and Bifidobacterium and metabolizing and detoxifying acetaldehyde, which is harmful to the human body generated in this process, has been conducted. reported

Among them, technologies related to hangover relief using Lactobacillus genus include Korean Patent Registration No. 10-2078324, "Composition for Relieving Hangover and Intestinal Trouble Containing Grain-derived Lactobacillus", and Korean Patent Registration No. 10-2078324. -1853603, 「Composition containing probiotics for decomposing alcohol or acetaldehyde」, etc. are disclosed.

Accordingly, the present inventors found that the Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain (accession number: KCTC 13840BP) isolated from breast milk has an aldehyde decomposition enzyme and has excellent alcohol decomposition ability, It was found that it inhibits damage and completed the present invention.

Republic of Korea Patent Registration No. 10-2078324 Republic of Korea Patent Registration No. 10-1853603

An object of the present invention is to provide a novel strain having a hangover relief effect.

Another object of the present invention is to provide a probiotics preparation containing the novel strain as an active ingredient.

Another object of the present invention is to provide a food composition for relieving hangover and improving alcoholic liver damage comprising the probiotics preparation.

In order to achieve the above object, the present invention provides a Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain (accession number: KCTC 13840BP) having alcohol metabolizing enzyme activity.

In the present invention, the strain is characterized in that it has an acetaldehyde decomposing effect and a liver function protecting activity against alcohol through aldehyde dehydrogenase (ALDH) activity.

In the present invention, the strain is characterized in that it is derived from breast milk.

In addition, the present invention is Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) ( Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY)) strain (accession number: KCTC 13840BP), a lysate of the strain, an extract of the strain, a culture of the strain, a concentrate of the culture And it provides a probiotics preparation containing at least one active ingredient selected from the group consisting of dry matter.

In the present invention, the strain is characterized in that it is prepared in the form of live cells by freeze-drying.

In the present invention, the probiotics preparation is characterized in that any one formulation selected from the group consisting of liquid, powder, granulated tablets and capsules.

In addition, the present invention provides a food composition for relieving hangover and improving alcoholic liver damage comprising the probiotics preparation.

In the present invention, the food composition is characterized in that it is included in any one product selected from the group consisting of food, food additives, beverages, beverage additives, fermented milk and health functional foods.

The novel Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain (accession number: KCTC 13840BP) provided by the present invention has an aldehyde dehydrogenase, so it has excellent acetaldehyde decomposition efficiency, and alcohol Since it has an excellent effect of protecting hepatocytes from toxicity by , it can be used as a food composition for relieving hangover and improving alcoholic liver damage.

1 is a nucleotide sequence of the 16S rRNA gene of the Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain (Accession Number: KCTC 13840BP).
Figure 2 shows the phylogenetic relationship with other bacteria based on the nucleotide sequence of the 16S rRNA gene of the Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) ( Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY)) strain (accession number: KCTC 13840BP) It is also
3 is an image confirming the presence or absence of the ALDH enzyme of the Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain (accession number: KCTC 13840BP).
Figure 4 is a graph showing the ALDH (aldehyde dehydrogenase) activity of the Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain (Accession Number: KCTC 13840BP).
5 is a graph showing the evaluation of liver cell toxicity of the Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) ( Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY)) strain (accession number: KCTC 13840BP).
6 is a graph showing the hepatocellular protective activity of the Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain (accession number: KCTC 13840BP) in an alcohol toxicity-induced model.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclatures used herein are those well known and commonly used in the art.

Throughout the present specification, when a certain component is said to "include", it means that it may further include other components without excluding other components unless otherwise stated.

Accordingly, in the present invention, as a result of 16S rRNA gene sequencing analysis from microorganisms isolated from breast milk, it was confirmed that the microorganisms belong to the genus Lactobacillus , and Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) ( Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY)) strain and deposited a patent with the Korea Research Institute of Bioscience and Biotechnology (KCTC) on April 22, 2019, and received accession number KCTC13840BP.

In addition, Aldehyde dehydrogenase (ALDH) (hereinafter abbreviated as 'ALDH') in the isolated novel microorganism Lactobacillus gasseri GFC_1220 (EJR_JOY) strain (Accession Number: KCTC 13840BP) It was confirmed that it possesses.

Accordingly, the present invention relates to a Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain (accession number: KCTC 13840BP) having alcohol metabolizing enzyme activity (hereinafter abbreviated as 'GFC_1220 (GFC_EJR_JOY)') .

The strain may have an acetaldehyde decomposing effect and a liver function protective activity against alcohol through aldehyde dehydrogenase (ALDH) activity.

At this time, the GFC_1220 (GFC_EJR_JOY) strain is characterized in that it is derived from breast milk.

In addition, the present invention is a probiotics preparation containing at least one active ingredient selected from the group consisting of the GFC_1220 (GFC_EJR_JOY) strain, a lysate of the strain, an extract of the strain, a culture of the strain, a concentrate of the culture, and a dried product. it's about

The GFC_1220 (GFC_EJR_JOY) strain may be prepared in the form of live cells by freeze-drying.

The medium for culturing the cells of the GFC_1220 (GFC_EJR_JOY) strain usually contains skim milk, whey, milk proteins such as casein, sugars, yeast extract, etc., and various general aerobic or anaerobic methods are used as culture methods. can be used appropriately.

As the culture temperature of the strain to obtain the culture solution of the GFC_1220 (GFC_EJR_JOY) strain, for example, set the culture temperature to 25 ~ 40 ℃, and during culture, use an alkali such as sodium hydroxide to change the pH of the medium from acidic to neutral. , For example, a neutralization culture method in which pH is maintained at about 5 to 6 may be used. In addition to the neutralization culture method, any culture method such as batch culture may be used, and after cultivation, the culture or its supernatant may be concentrated, dried, diluted, etc., if necessary.

In addition, the supernatant of the culture and the cells may be separated using a centrifugal separation method or a membrane separation method, and the cells may be recovered in a concentrated state. In addition, the cells may be treated with ultrasonic waves or enzymes to extract components from the cells, or the cultures or supernatants thereof, or cells or extracts thereof may be dried. These may be included as active ingredients of the probiotics preparation.

In the present invention, "Probiotics" is a live microbial food supplement that has a beneficial effect on the water column by improving the microbial balance of the intestine, and more broadly, when consumed in a specific number, provides health effects beyond the original basic nutrition. can be defined as living microorganisms.

The probiotics formulation may be any one formulation selected from the group consisting of liquid, powder, granules, tablets and capsules.

In addition, the present invention relates to a food composition for relieving hangover and improving alcoholic liver damage comprising the probiotics preparation.

The food composition may be any one product selected from the group consisting of food, food additives, beverages, beverage additives, fermented milk, and health functional foods. When used as a product as above, various foods, fermented milk, meat, beverages, chocolate, snacks, confectionery, pizza, ramen, other noodles, chewing gum, ice cream, alcoholic beverages, vitamin complexes, alcoholic beverages and other health functional foods It may, but is not limited thereto.

The food composition may include ingredients commonly added during food preparation. Examples include proteins, carbohydrates, fats, nutrients, seasonings and flavors. Examples of the carbohydrate include common sugars such as monosaccharides (eg glucose, fructose, etc.), disaccharides (eg maltose, sucrose, oligosaccharides, etc.) and polysaccharides (eg dextrin, cyclodextrin, etc.) and xylitol, sorbitol , and sugar alcohols such as erythritol. As flavoring agents, natural flavoring agents (taumarin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.

For example, when the food composition of the present invention is prepared as a drink, it may further include citric acid, high fructose corn syrup, sugar, glucose, acetic acid, malic acid, fruit juice, plum extract or barnacle fruit extract.

The food composition of the present invention includes processed forms of all natural materials such as food, functional food, nutritional supplement, health food and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

For example, as a health food, the GFC_1220 (GFC_EJR_JOY) strain itself can be prepared and consumed in the form of tea, juice and drink, or granulated, encapsulated and powdered to be consumed. In addition, as foods, beverages (including alcoholic beverages), fruits and their processed foods (for example, canned fruits, bottled products, jams, marmalades, etc.), fish, meat and their processed foods (for example, ham, sausages, corned beef, etc.) , Breads and noodles (e.g. udon, buckwheat noodles, ramen, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, taffy, dairy products (e.g. yogurt, fermented milk, butter, cheese, etc.), edible vegetable oil, margarine , It can be prepared by adding vegetable protein, retort food, frozen food, various seasonings (eg, soybean paste, soy sauce, sauce, etc.). In addition, in order to use the GFC_1220 (GFC_EJR_JOY) strain of the present invention in the form of a food additive, it can be prepared and used in the form of a powder or concentrate.

The amount of the food composition can be appropriately adjusted according to individual differences such as age, health status, dosage form, and form, and can be usefully used as a food composition for relieving hangover and improving alcoholic liver damage.

The composition obtained from the method described above has excellent aldehyde dehydrogenase activity, effectively decomposes acetaldehyde, and exhibits an effect of protecting hepatocytes from alcohol-induced toxicity, thereby providing an excellent food for relieving hangover and improving alcoholic liver damage.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for explaining the present invention in more detail, and it is to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. It will be self-evident.

<Example 1> Lactobacillus gasseri GFC_1220 (EJR_JOY) ( Lactobacillus gasseri Isolation and identification of GFC_1220 (GFC_EJR_JOY))

1-1. Isolation of strains

In order to isolate the novel lactic acid strain according to the present invention, breast milk was obtained and suspended in PBS buffer. Thereafter, 0.1 ml was inoculated into MRS agar medium (Difco), incubated aerobically and anaerobically at 37° C. for about 48 hours, and strains forming colonies were isolated.

1-2. Identification of the strain

By analyzing the 16S rRNA gene nucleotide sequence of the isolated strain, the species of the strain was confirmed and the strain name was given. As a result of strain identification (NCBI), the isolated strain confirmed 99.72% homology with Lactobacillus gasseri , and the strain name was Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) and the nucleotide sequence (SEQ ID NO: 1) of the 16S rRNA gene is shown in FIG. Figure 2 shows the phylogenetic relationship with other bacteria based on the nucleotide sequence of the 16S rRNA gene.

1-3. Accession information of the strain

The inventors of the present invention patented the Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain to the Korea Research Institute of Bioscience and Biotechnology (KCTC) on April 22, 2019, and received accession number KCTC13840BP.

1-4. Confirmation of the presence of ALDH enzyme in the isolated strain

In order to confirm the presence or absence of the ALDH enzyme in the isolated strain, Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) ( Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY)), the strain was cultured on MRS agar medium (MRS agar medium, Difco), and 48 hours after culture, single The colony was diluted in sterilized purified water and mixed with ALDH primers, SEQ ID NO: 2 (ALDH2(R): 5'-AGGAATGTTCCAAGCACCAC-3') and SEQ ID NO: 3 (ALDH2(F): 5'-GTTGCCATCATTGACACTTGGT-3') and mixed with 95 After pre-denaturation at ° C for 5 minutes, denaturation at 95 ° C for 1 minute, attachment at 55 ° C for 1 minute, extension at 72 ° C for 1 minute was repeated 30 times, followed by post-extension at 72 ° C for 10 minutes did It was shown in Figure 3 that ALDH was retained in the strain by electrophoresis on a 1.2% agarose gel.

<Comparative Example 1> Lactobacillus gasseri Standard strain preparation

A Lactobacillus gasseri standard strain (KACC No. 12424) was purchased from the Korean Agricultural Culture Collection (KACC) of the National Institute of Agricultural Sciences and used as a comparative example.

<Experimental Example 1> ALDH enzyme activity test: ALDH activity assay

ALDH activity was evaluated to determine ALDH enzyme activity using the strains of Example 1 and Comparative Example 1.

After activating the strains of Example 1 and Comparative Example 1 and incubating them at a concentration of 1X10 ⁸ CFU/mL, the reaction solution was placed in a 96-well plate using an ALDH activity assay kit (Abcam) and analyzed at 450 nm using an ELISA reader. Absorbance was measured in kinetic mode for 30 minutes. In addition, the ALDH activity was corrected by the protein amount of each strain using the Bradford (Bio-rad) method. Therefore, the ALDH activity (mU/mg) of the sample was calculated as shown in Equation 1 below based on the amount of protein and 0 minute of reaction for each sample.

[Equation 1]

ALDH activity (mU/mg) = [(amount of NADH produced during reaction by sample)/(reaction time X amount of sample reacted)] X (dilution factor / amount of protein in sample)

ALDH (mU/mL) ALDH activity (mU/mg) Example 1 3.130 963.438 Comparative Example 1 1.994 818.898

As a result, as can be seen in Table 1 and FIG. 4, it was confirmed that Example 1 had significantly better ALDH activity than Comparative Example 1.

<Experimental Example 2> Cytotoxicity evaluation: MTT assay

In order to confirm whether the strains of Example 1 and Comparative Example 1 have toxicity to cells, a cytotoxicity test was conducted using HepG2 cells, a human-derived liver cancer cell line.

After activating and culturing the strains of Example 1 and Comparative Example 1, they were collected by centrifugation and washed, and the recovered cells were rapidly frozen at -80 ° C, lyophilized at -80 ° C, pulverized and stored in a refrigerator. .

In order to determine whether it is toxic to HepG2 cells, a human-derived liver cancer cell line, the cells were dispensed in a 24-well plate at a concentration of 1X10 ⁵ cell/well using MEM medium, and then maintained at 37°C and 5% CO ₂ for 24 hours. cultured. The lyophilized strains of Example 1 and Comparative Example 1 were mixed with the medium by concentration to the cell cultured for 24 hours, treated in each well, cultured for 24 hours at 37 ° C and 5% CO ₂ conditions, and then the culture medium After removal, each well was treated with 0.5mg/mL MTT solution and reacted in an incubator for 4 hours. After 4 hours, the supernatant was removed, and 1 mL of DMSO was added to each well to dissolve the formazan crystals, and the absorbance was measured at 560 nm with an ELISA reader. The cell viability was calculated as shown in Equation 2 below. .

[Equation 2]

Cell viability (%) = [(absorbance of sample treated group / absorbance of untreated group) X 100]

As a result, as can be seen in FIG. 5, it was confirmed that Example 1 and Comparative Example 1 did not show cytotoxicity.

<Experimental Example 3> Hepatocellular protective activity against alcohol-induced liver damage: MTT assay

In order to confirm whether the strains of Example 1 and Comparative Example 1 have a hepatocellular protective effect against alcohol-induced liver damage, a hepatocellular protective activity test was conducted using HepG2 cells, a human-derived liver cancer cell line.

After activating and culturing the strains of Example 1 and Comparative Example 1, they were collected by centrifugation and washed, and the recovered cells were rapidly frozen at -80 ° C, lyophilized at -80 ° C, pulverized and stored in a refrigerator. .

In order to confirm the protective effect on HepG2 cells induced by alcoholic liver damage, the cells were dispensed at a concentration of 1X10 ⁵ cell/well in a 24-well plate using MEM medium, and then at 37 °C and 5% CO ₂ conditions. Incubated for 24 hours. After mixing the strains of Example 1 and Comparative Example 1 with a medium containing 500 mM alcohol for each concentration in the cells cultured for 24 hours, each well was treated, and then cultured for 24 hours at 37 ° C and 5% CO ₂ conditions to prevent alcoholic liver damage. After induction, the culture medium was removed, and each well was treated with 0.5 mg/mL of MTT solution and allowed to react in an incubator for 4 hours. After 4 hours, the supernatant was removed and 1 mL of DMSO was added to each well to dissolve the formazan crystals, and the absorbance was measured at 560 nm with an ELISA reader, and the hepatocellular protective activity was calculated as shown in Equation 3 below. did

[Equation 3]

Hepatocellular protective activity (%) = [(absorbance of alcohol-treated and sample-treated groups/absorbance of alcohol-free and sample-treated groups) X 100]

As a result, as can be seen in FIG. 6 , it was confirmed that Example 1 had better hepatocellular protective activity in HepG2 cells damaged by alcohol than Comparative Example 1.

<Formulation Example 1> Lactobacillus gasseri GFC_1220 (EJR_JOY) ( Lactobacillus gasseri Preparation of probiotics preparation of GFC_1220 (GFC_EJR_JOY))

The GFC_1220 (EJR_JOY) strain obtained in Example 1 was washed twice with distilled water, suspended in distilled water, and lyophilized to prepare a probiotics preparation in the form of a final powder.

<Formulation Example 2> Preparation of health functional food powder

Formulation Example 1 --------------------------------------- 20mg

Lactose ---------------------------------------------- 100mg

Talc ---------------------------------------------- 10mg

The above components are mixed and filled in airtight bags to prepare a powder.

<Formulation Example 3> Manufacture of health functional food tablets

Formulation Example 1 --------------------------------------- 10mg

Corn Starch ------------------------------------ 100mg

Lactose ---------------------------------------------- 100mg

Magnesium Stearate ---------------------------- 2mg

After mixing the above ingredients, tablets are prepared by tableting according to a conventional tablet manufacturing method.

<Formulation Example 4> Preparation of beverage for relieving hangover

Formulation Example 1 --------------------------------------- 0.02g

Citric Acid ------------------------------ 0.25g

Fructose -------------------------------------------- 35g

Lemon juice ---------------------------------------- 60g

After mixing the above components, a beverage for relieving hangover was prepared according to a conventional beverage manufacturing method.

<Formulation Example 5> Preparation of chewing gum for relieving hangover

Formulation Example 1 --------------------------------------- 0.02g

Gum Base ------------------------------------------ 26.1g

Sugar ------------------------------------------- 70.9g

Spices -------------------------------------------- 1g

Water ---------------------------------------------- 2g

After mixing the above components, chewing gum for relieving hangover was prepared according to a conventional chewing gum method.

Having described specific parts of the present invention in detail above, it will be clear to those skilled in the art that these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby. will be. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Korea Research Institute of Bioscience and Biotechnology (KTCT) KCTC13840BP 20190422

<110> GFC Life Science Co., Ltd. <120> Lactobacillus gasseri GFC_1220(GFC_EJR_JOY) for alcohol metabolic enzyme activity and food composition comprising the same as an effective ingredient <130> P20264 <160> 3 <170> KoPatentIn 3.0 <210> 1 <211> 1022 <212> DNA <213> artificial sequence <220> <223> Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) <400> 1 ttagacggct gactcctata aaggttatcc caccggcttt gggtgttaca gactctcatg 60 gtgtgacggg cggtgtgtac aaggcccggg aacgtattca ccgcggcgtg ctgatccgcg 120 attactagcg attccagctt cgtgtaggcg agttgcagcc tacagtccga actgagaacg 180 gctttcagag atccgcttgc cttcgcaggt tcgcttctcg ttgtaccgtc cattgtagca 240 cgtggtgtagc ccaggtcata aggggcatga tgacttgacg tcatccccac cttcctccgg 300 tttgtcaccg gcagtctcat tagagtgccc aacttaatga tggcaactaa tgacaagggt 360 tgcgctcgtt gcgggactta acccaacatc tcacgacacg agctgacgac agccatgcac 420 cacctgtctc agcgtccccg aagggaactc ctaatctctt aggtttgcac tggatgtcaa 480 gacctggtaa ggttcttcgc gttgcttcga attaaaccac atgctccacc gcttgtgcgg 540 gccccccgtca attcctttga gtttcaacct tgcggtcgta ctccccaggc ggaggtgctta 600 atgcgttagc tgcagcactg agaggcggaa acctcccaac acttagcact catcgtttac 660 ggcatggact accagggtat ctaatcctgt tcgctaccca tgctttcgag cctcagcgtc 720 agttgcagac cagagagccg ccttcgccac tggtgttctt ccatatatct acgcattcca 780 ccgctacaca tggagttcca ctctcctctt ctgcactcaa gttcaacagt ttctgatgca 840 attctccggt tgagccgaag gctttcacat cagacttatt gaaccgcctg cactcgcttt 900 acgcccaata aatccggaca acgcttgcca cctacgtatt accgcggctg ctggcacgta 960 gttagccgtg actttctaag taattacgt caaataaagg ccagttacta cctctatctt 1020 tc 1022 <210> 2 <211> 20 <212> DNA <213> artificial sequence <220> <223> ALDH2(R) <400> 2 aggaatgttc caagcaccac 20 <210> 3 <211> 22 <212> DNA <213> artificial sequence <220> <223> ALDH2 (F) <400> 3 gttgccatca ttgacacttg gt 22

Claims (8)

Breast milk-derived Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) strain having alcohol metabolizing enzyme activity (Accession No.: KCTC 13840BP).
The method of claim 1, wherein the strain has an acetaldehyde decomposition effect by aldehyde dehydrogenase (ALDH) activity and a liver function protective activity against alcohol Lactobacillus gasseri GFC_1220 (GFC_EJR_JOY) (GFC_EJR_JOY)) strain (accession number: KCTC 13840BP).
delete A probiotics preparation comprising at least one active ingredient selected from the group consisting of the strain of any one of claims 1 and 2, a lysate of the strain, an extract of the strain, a culture of the strain, a concentrate of the culture, and a dried product. .
The probiotics preparation according to claim 4, wherein the strain is freeze-dried and prepared in the form of live cells.
The probiotics preparation according to claim 4, wherein the probiotics preparation is any one formulation selected from the group consisting of liquid, powder, granulated tablets and capsules.
A food composition for relieving hangover and improving alcoholic liver damage comprising the probiotics preparation of claim 4.
The method of claim 7, wherein the food composition is for relieving hangover and improving alcoholic liver damage, characterized in that it is included in any one product selected from the group consisting of food, food additives, beverages, beverage additives, fermented milk and health functional foods. food composition.