KR102567791B1 - Lactobacillus plantarum GFC_B001 for removing hangover and food composition comprising the same as an effective ingredient - Google Patents

Abstract

The present invention relates to a Lactobacillus plantarum GFC_B001 (Accession Number: KCTC 14244BP) strain having a hangover relief effect and a food composition containing the same as an active ingredient.
The novel Lactobacillus plantarum GFC_B001 strain (accession number: KCTC 14244BP) provided by the present invention has an aldehyde dehydrogenase, so it has an excellent acetaldehyde decomposition effect, and is free from toxicity caused by alcohol. Since it is excellent in protecting liver cells, it can be used as a food composition for relieving hangover and improving alcoholic liver damage.

Description

Lactobacillus plantarum GFC_B001 strain having a hangover relief effect and a food composition comprising the same as an active ingredient

The present invention relates to a Lactobacillus plantarum GFC_B001 strain (Accession Number: KCTC 14244BP) having a hangover relief effect and a food composition containing the same as an active ingredient.

Usually, a hangover refers to symptoms such as headache, thirst, dizziness, and heartburn that begin within a few hours after drinking alcohol. It is caused by the toxicity of (alcohol) or acetaldehyde.

Alcohol, the main component of alcohol, is absorbed from the stomach or small intestine after ingestion and transported to the liver by blood. Alcohol thus transported is converted to acetaldehyde by metabolism in the liver. Alcohol dehydrogenase (ADH) is present in liver cells to oxidize alcohol to acetaldehyde, and acetaldehyde is decomposed into acetic acid by acetaldehyde dehydrogenase (ALDH), which is then transported to the muscles or adipose tissue of the advancing body. In this process, unoxidized acetaldehyde accumulates, which interferes with normal metabolism due to its toxicity, resulting in hangover symptoms such as headache, heartburn, and vomiting.

On the other hand, among microorganisms, bacteria that produce a lot of lactic acid as a metabolite while growing are collectively referred to as lactic acid bacteria. Lactic acid bacteria basically produce lactic acid, suppress the activity of harmful bacteria, and help maintain normal intestinal peristalsis. In particular, lactic acid bacteria settled in the intestine prevent disease occurrence by preventing pathogenic bacteria from attaching to the digestive tract epithelium, and antibiotics produced by lactic acid bacteria kill or inhibit the proliferation of pathogenic microorganisms or intestinal harmful bacteria that cause diarrhea.

Lactic acid bacteria were first identified by Pasteur in the process of researching the cause of wine rancidity in 1858, and later recognized as GRAS for use as a food additive by the US FDA, and are widely used in almost all food industries. there is. Lactic acid bacteria have long played an important role in dairy products such as yogurt, butter, and cheese, and are also present as major constituents of fermented foods such as soy sauce, kimchi, and soybean paste. Fermented foods using lactic acid bacteria exhibit symbolic and nutritional excellence, such as unique flavor, excellent preservability due to the produced lactic acid, and improved digestion and absorption by partial degradation of protein, forming a huge market size throughout the world.

Among the various genera currently used as lactic acid bacteria, the technology applied to various foods using the genus Lactobacillus includes Korean Registered Patent Publication No. 10-1917822, 「Lactobacillus plantarum KCCM 11322 strain fortified cirsimaritin-enhanced milk thistle Manufacturing method of concentrate”, Korean Patent Registration No. 10-1925997, “Lactobacillus plantarum wikim0067 with high sugar tolerance and fermented food using the same”, Korean Patent Registration No. 10-2065585, “Lactobacillus plantarum 3JSRL 24- Method for producing fermented brown rice sikhye using 4 strains”, Korean Patent Registration No. 10-1789155, “Lactobacillus paracasei strain having high viable cell count during fermentation, composition containing the same, and fermented food manufacturing method using the same”, etc. has been initiated.

Accordingly, the present inventors found that the Lactobacillus plantarum GFC_B001 strain (Accession Number: KCTC 14244BP) derived from the brewery has an aldehyde dehydrogenase activity, and thus has excellent acetaldehyde decomposition efficiency, and is induced by alcohol. The present invention was completed by discovering that it can impart an effect of protecting liver function from hepatotoxicity.

Republic of Korea Patent Publication No. 10-1917822 Republic of Korea Patent Registration No. 10-1925997 Republic of Korea Patent Registration No. 10-2065585 Republic of Korea Patent Registration No. 10-1789155

An object of the present invention is to provide a novel strain having a hangover relief effect.

Another object of the present invention is to provide a probiotics preparation containing the novel strain as an active ingredient.

Another object of the present invention is to provide a food composition for relieving hangover and improving alcoholic liver damage comprising the probiotics preparation.

In order to achieve the above object, the present invention is a Lactobacillus plantarum GFC_B001 ( Lactobacillus plantarum GFC_B001) strain (accession number: KCTC 14244BP) having aldehyde dehydrogenase activity, acetaldehyde decomposition effect and liver function protection activity against alcohol provides

In the present invention, the strain is characterized in that it is isolated from the brewery.

In addition, the present invention is a group consisting of a Lactobacillus plantarum GFC_B001 (Accession Number: KCTC 14244BP) strain, a lysate of the strain, an extract of the strain, a culture of the strain, a concentrate of the culture, and a dried product. It provides a probiotics preparation containing one or more active ingredients selected from.

In the present invention, the strain is characterized in that it is prepared in the form of live cells by freeze-drying.

In the present invention, the probiotics preparation is characterized in that any one formulation selected from the group consisting of liquid, powder, granule, tablet and capsule.

In addition, the present invention provides a food composition for relieving hangover and improving alcoholic liver damage comprising the probiotics preparation.

In the present invention, the food composition is characterized in that it is included in any one product selected from the group consisting of food, food additives, beverages, beverage additives, fermented milk and health functional foods.

The novel Lactobacillus plantarum GFC_B001 strain (accession number: KCTC 14244BP) provided by the present invention has an aldehyde dehydrogenase, so it has excellent acetaldehyde decomposition efficiency, and is free from alcohol-induced toxicity. Since it has an excellent protective effect, it can be used as a food composition for relieving hangover and improving alcoholic liver damage.

1 is a nucleotide sequence of the 16S rRNA gene of the Lactobacillus plantarum GFC_B001 strain (accession number: KCTC 14244BP).
Figure 2 is a diagram showing the phylogenetic relationship with other bacteria based on the nucleotide sequence of the 16S rRNA gene of the Lactobacillus plantarum GFC_B001 strain (accession number: KCTC 14244BP).
3 is a graph showing the ALDH (aldehyde dehydrogenase) activity of the Lactobacillus plantarum GFC_B001 strain (accession number: KCTC 14244BP).
4 is a graph showing the degree of acetaldehyde degradation of the Lactobacillus plantarum GFC_B001 strain (accession number: KCTC 14244BP).
5 is a graph showing the liver cell toxicity evaluation of the Lactobacillus plantarum GFC_B001 strain (accession number: KCTC 14244BP).
6 is a graph showing the hepatocellular protective activity of the Lactobacillus plantarum GFC_B001 strain (accession number: KCTC 14244BP) against an alcohol toxicity induced model.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclatures used herein are those well known and commonly used in the art.

Throughout the present specification, when a certain component is said to "include", it means that it may further include other components without excluding other components unless otherwise stated.

Therefore, in the present invention, strains with excellent ALDH activity are selected by screening strains having aldehyde dehydrogenase (ALDH) (hereinafter abbreviated as 'ALDH') from microorganisms isolated from breweries, and 16S RNA gene base As a result of sequence analysis, it was confirmed that the microorganism was a novel microorganism belonging to the genus Lactobacillus . Accordingly, it was named Lactobacillus plantarum GFC_B001 ( Lactobacillus plantarum GFC_B001) strain and patented with the Korea Research Institute of Bioscience and Biotechnology (KCTC) on July 20, 2020, and was given accession number KCTC 14244BP.

Therefore, the present invention is a Lactobacillus plantarum GFC_B001 strain (accession number: KCTC 14244BP) (hereinafter abbreviated as 'GFC_B001') having aldehyde dehydrogenase activity, acetaldehyde decomposition efficacy, and liver function protection activity against alcohol. ) is about.

At this time, the GFC_B001 strain is characterized in that it is isolated from the brewery.

In addition, the present invention relates to a probiotic preparation comprising at least one active ingredient selected from the group consisting of the GFC_B001 strain, a shellfish solution of the strain, an extract of the strain, a concentrate and a dried product of the culture solution of the strain.

The GFC_B001 strain may be prepared in the form of live cells by lyophilization.

The medium for culturing the cells of the GFC_B001 strain usually includes skim milk, whey, milk proteins such as casein, sugars, yeast extract, etc., and various general aerobic or anaerobic methods can be used as appropriate there is.

As the culture temperature of the strain to obtain the culture solution of the GFC_B001 strain, for example, the culture temperature is set to 25 ~ 40 ℃, and during culture, the pH of the medium is adjusted from acidic to neutral by using an alkali such as sodium hydroxide. For example, a neutralization culture method in which the pH is maintained at about 5 to 6 may be used. In addition to the neutralization culture method, any culture method such as batch culture may be used, and after cultivation, the culture or the supernatant may be concentrated, dried, diluted, etc., if necessary.

In addition, the supernatant of the culture and the cells may be separated using a centrifugal separation method or a membrane separation method, and the cells may be recovered in a concentrated state. In addition, the cells may be treated with ultrasonic waves or enzymes to extract components from the cells, or the cultures or supernatants thereof, or cells or extracts thereof may be dried. These may be included as active ingredients of the probiotics preparation.

The probiotics formulation may be any one formulation selected from the group consisting of liquid, powder, granules, tablets and capsules.

In addition, the present invention relates to a food composition for relieving hangover and improving alcoholic liver damage comprising the probiotics preparation.

The food composition may be any one product selected from the group consisting of food, food additives, beverages, beverage additives, fermented milk, and health functional foods. When used as a product as above, various foods, fermented milk, meat, beverages, chocolate, snacks, confectionery, pizza, ramen, other noodles, chewing gum, ice cream, alcoholic beverages, vitamin complexes, alcoholic beverages and other health functional foods It may, but is not limited thereto.

The food composition may include ingredients commonly added during food preparation. Examples include proteins, carbohydrates, fats, nutrients, seasonings and flavors. Examples of the carbohydrate include monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, oligosaccharides, etc.) and polysaccharides (eg, dextrin, cyclodextrin, etc.), and xylitol, sorbitol , and sugar alcohols such as erythritol. As flavoring agents, natural flavoring agents (taumarin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.

For example, when the food composition of the present invention is prepared as a drink, it may further include citric acid, high fructose corn syrup, sugar, glucose, acetic acid, malic acid, fruit juice, plum extract or barnacle fruit extract.

The food composition of the present invention includes processed forms of all natural materials such as food, functional food, nutritional supplement, health food and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

For example, as a health food, the GFC_B001 strain itself can be prepared and consumed in the form of tea, juice, or drink, or can be granulated, encapsulated, or powdered to be consumed. In addition, as foods, beverages (including alcoholic beverages), fruits and their processed foods (for example, canned fruits, bottled products, jams, marmalades, etc.), fish, meat and their processed foods (for example, ham, sausages, corned beef, etc.) , Breads and noodles (e.g. udon, buckwheat noodles, ramen, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, taffy, dairy products (e.g. yogurt, fermented milk, butter, cheese, etc.), edible vegetable oil, margarine , It can be prepared by adding vegetable protein, retort food, frozen food, various seasonings (eg, soybean paste, soy sauce, etc.). In addition, in order to use the GFC_B001 strain of the present invention in the form of a food additive, it can be prepared and used in the form of a powder or concentrate.

The amount of the food composition can be appropriately adjusted according to individual differences such as age and health status, dosage form, and form, and can be usefully used as a food composition for relieving hangover and improving alcoholic liver damage.

The composition obtained from the method described above has excellent aldehyde dehydrogenase activity, effectively decomposes acetaldehyde, and exhibits an effect of protecting hepatocytes from alcohol-induced toxicity, thereby providing an excellent food for relieving hangover and improving alcoholic liver damage. .

Hereinafter, the present invention will be described in more detail through examples. These examples are only for explaining the present invention in more detail, and it is to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. It will be self-evident.

<Example 1> Lactobacillus Plantarum GFC_B001 ( Lactobacillus plantarum Isolation and identification of GFC_B001)

1-1. Isolation of strains

In order to isolate the novel lactic acid bacteria strain according to the present invention, the brewery (Pocheon-si, Gyeonggi-do) makgeolli was inoculated into MRS agar medium (Digco), incubated anaerobically at 37 ° C for about 48 hours, and then colonies The strains that formed were isolated.

1-2. Selection of lactic acid bacteria with ALDH enzyme

In order to confirm the presence or absence of the ALDH enzyme of the isolated lactic acid bacteria, the strain was cultured at a concentration of 1X10 ⁸ CFU / mL, and the ALDH activity was confirmed using an ALDH activity assay kit (Adcam), and the strain with the highest activity was selected.

1-3. Identification of selected strains

The 16S rRNA gene nucleotide sequence of the selected strain was analyzed to determine the species of the strain, and the strain name was assigned. As a result of strain identification, the selected strain was confirmed to have 99.87% homology with Lactobacillus plantarum , and the strain was named Lactobacillus plantarum GFC_B001, and the base of the 16S rRNA gene The sequence (SEQ ID NO: 1) is shown in FIG. 1 . Figure 2 shows the phylogenetic relationship with other bacteria based on the nucleotide sequence of the 16S rRNA gene.

1-4. Accession information of the strain

The inventors of the present invention patented the Lactobacillus plantarum GFC_B001 strain to the Korea Research Institute of Bioscience and Biotechnology (KCTC) on July 20, 2020, and received accession number KCTC 14244BP.

<Example 2> Lactobacillus plantarum GFC_B001 ( Lactobacillus plantarum GFC_B001) Probiotics Preparation

The GFC_B001 strain obtained in Example 1 was washed twice with distilled water, suspended in distilled water, and lyophilized to prepare a probiotics preparation in the form of a final powder.

<Comparative Example 1> Lactobacillus plantarum Standard strain preparation

A Lactobacillus plantarum standard strain (KACC No. 11451) was received from the Korean Agricultural Culture Collection (KACC) of the National Institute of Agricultural Sciences and used as a comparative example.

<Experimental Example 1> ALDH enzyme activity test: ALDH activity assay

ALDH activity was evaluated to determine ALDH enzyme activity using the strains of Example 1 and Comparative Example 1.

After activating the strains of Example 1 and Comparative Example 1 and incubating them at a concentration of 1X10 ⁸ CFU/mL, the reaction solution was placed in a 96-well plate using an ALDH activity assay kit (Abcam) and analyzed at 450 nm using an ELISA reader. Absorbance was measured in kinetic mode for 30 minutes. In addition, the ALDH activity was corrected by the protein amount of each strain using the Bradford (Bio-rad) method. Therefore, the ALDH activity (mU/mg) of the sample was calculated as in Equation 1 based on the amount of protein for each sample and the reaction time of 0 minutes.

[Equation 1]

ALDH activity (mU/mg) = [(amount of NADH produced during reaction by sample)/(reaction time X amount of sample reacted)] X (dilution factor / amount of protein in sample)

As a result, as can be seen in FIG. 3, it was confirmed that Example 1 had significantly better ALDH activity than Comparative Example 1.

<Experimental Example 2> Acetaldehyde decomposition confirmation test: acetaldehyde quantitative assay

In order to determine the acetaldehyde decomposition effect using the strains of Example 1 and Comparative Example 1, the acetaldehyde content was measured.

After activating the strains of Example 1 and Comparative Example 1 and incubating them at a concentration of 1X10 ⁸ CFU/mL, 1 mM acetaldehyde was added to each strain at the same concentration and allowed to react for 30 minutes. After 30 minutes, the reaction solution reacted for 30 minutes using an acetaldehyde assay kit (Bio assay systems) was placed in a 96-well plate and absorbance was measured at 565 nm using an ELISA reader.

As a result, as can be seen in FIG. 4, it was confirmed that the acetaldehyde content of Example 1 was reduced compared to Comparative Example 1, and the acetaldehyde decomposition effect was better.

<Experimental Example 3> Cytotoxicity evaluation: MTT assay

In order to confirm whether the strains of Example 1 and Comparative Example 1 have toxicity to cells, a cytotoxicity test was conducted using HepG2 cells, a human-derived liver cancer cell line.

After activating and culturing the strains of Example 1 and Comparative Example 1, they were collected by centrifugation and washed, and the recovered cells were rapidly frozen at -80 ° C, lyophilized at -80 ° C, pulverized and stored in a refrigerator. .

In order to determine whether the cells are toxic to HepG2 cells, a human-derived liver cancer cell line, the cells were dispensed in a 24-well plate at a concentration of 1X10 ⁸ cells/well using MEM medium, and cultured for 24 hours at 37°C and 5% CO₂ conditions. did After mixing the lyophilized strains of Example 1 and Comparative Example 1 with the culture medium by concentration in the cells cultured for 24 hours, each well was treated, cultured for 24 hours at 37 ° C and 5% CO₂ conditions, and then the culture medium was removed. Then, each well was treated with 0.5 mg/mL MTT solution and reacted in an incubator for 4 hours. After 4 hours, the supernatant was removed, and 1 mL of DMSO was added to each well to dissolve the formazan crystals, and the absorbance was measured at 560 nm with an ELISA reader. The cell viability was calculated as shown in Equation 2 below. .

[Equation 2]

Cell viability (%) = [(absorbance of sample treated group / absorbance of untreated group) X 100]

As a result, as can be seen in FIG. 5, it was confirmed that Example 1 and Comparative Example 1 did not exhibit cytotoxicity.

<Experimental Example 4> Hepatocellular protective activity against alcohol-induced liver damage: MTT assay

In order to confirm whether the strains of Example 1 and Comparative Example 1 have a hepatocellular protective effect against alcohol-induced liver damage, a hepatocellular protective activity test was conducted using HepG2 cells, a human-derived liver cancer cell line.

After activating and culturing the strains of Example 1 and Comparative Example 1, centrifuged, recovered and washed, and the recovered cells were rapidly frozen at -80 ° C, freeze-dried at -80 ° C temperature conditions, then pulverized and stored in a refrigerator did

In order to confirm the protective effect on HepG2 cells induced by alcoholic liver damage, the cells were dispensed at a concentration of 1X10 ⁸ cell/well in a 24-well plate using MEM medium, and then cultured at 37℃ and 5% CO₂ for 24 incubated for a period of time. After mixing the strains of Example 1 and Comparative Example 1 with a medium containing 500 mM alcohol for each concentration in the cell cultured for 24 hours, treatment was performed in each well, followed by culturing for 24 hours at 37 ° C and 5% CO₂ conditions to prevent alcoholic liver damage. After induction, the culture medium was removed, and each well was treated with 0.5 mg/mL MTT solution and allowed to react in an incubator for 4 hours. After 4 hours, the supernatant was removed and 1 mL of DMSO was added to each well to dissolve the formed formazan crystals, and the absorbance was measured at 560 nm with an ELISA reader, and the hepatocellular protective activity was calculated as in Equation 3 below. did

[Equation 3]

Hepatocellular protective activity (%) = [(absorbance of alcohol-treated and sample-treated groups/absorbance of alcohol-free and sample-treated groups) X 100]

As a result, as can be seen in FIG. 6 , it was confirmed that Example 1 had better hepatocellular protective activity in HepG2 cells damaged by alcohol than Comparative Example 1.

<Formulation Example 1> Preparation of lactic acid bacteria beverage

A lactic acid bacteria beverage was prepared by a conventional method using the ingredients shown in Table 1 below.

ingredient Content (weight g) Probiotics of Example 2 2 Hydroxypropyl Starch 6 lactose One water 200

<Formulation Example 2> Manufacture of ice cream for relieving hangover and improving alcoholic liver damage

A health drink for relieving hangover and improving alcoholic liver damage was prepared by a conventional method using the ingredients shown in Table 2 below.

ingredient Content (weight g) Probiotics of Example 2 0.2 citric acid One oligosaccharide 100 taurine One Purified water 0.2

<Formulation Example 3> Manufacture of candy for relieving hangover and improving alcoholic liver damage

Candy for relieving hangover and improving alcoholic liver damage was prepared by a conventional method using the ingredients shown in Table 3 below.

ingredient Content (weight g) Probiotics of Example 2 2 glucose 11 citric acid 0.38 vitamin C 0.04 Stevioside 0.035 synthetic flavoring agent 0.18 Purified water 86.35

Having described specific parts of the present invention in detail above, it will be clear to those skilled in the art that these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby. will be. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

Korea Research Institute of Bioscience and Biotechnology (KCTC) KCTC14244BP 20200720

<110> GFC Life Science Co., Ltd. <120> Lactobacillus plantarum GFC_B001 for removing hangover and food composition comprising the same as an effective ingredient <130> P20265 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1453 <212> DNA <213> artificial sequence <220> <223> Lactobacillus plantarum GFC_B001 <400> 1 ccgcgcgctc tatctgcagt cgacgaactc tggtattgat tggtgcttgc atcatgattt 60 acatttgagt gagtggcgaa ctggtgagta acacgtggga aacctgccca gaagcggggg 120 ataaccctg gaaacagatg ctaataccgc ataacaactt ggaccgcatg gtccgagctt 180 gaaagatggc ttcggctatc acttttggat ggtcccgcgg cgttatagct agatggtggg 240 gtaacggctc accatggcaa tgatacgtag ccgacctgag agggtaatcg gccacattgg 300 gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc cacaatggac 360 gaaagtctga tggagcaacg ccgcgtgagt gaagaagggt ttcggctcgt aaaactctgt 420 tgttaaagaa gaacatatct gagagtaact gttcaggtat tgacggtatt taaccagaaa 480 gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttgtccgga 540 tttatgggc gtaaagcgag cgcaggcggt tttttaagtc tgatgtgaaa gccttcggct 600 caaccgaaga agtgcatcgg aaactgggaa acttgagtgc agaagaggac agtggaactc 660 catgtgtagc ggtgaaatgc gtagatatat ggaagaacac cagtggcgaa ggcggctgtc 720 tggtctgtaa ctgacgctga ggctcgaaag tatgggtagc aaacaggatt agataccctg 780 gtagtccata ccgtaaacga tgaatgctaa gtgttggagg gtttccgccc ttcagtgctg 840 cagctaacgc attaagcatt ccgcctgggg agtacggccg caaggctgaa actcaaagga 900 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagct acgcgaagaa 960 cctaccagg tcttgacata ctatgcaaat ctaagagatt agacgttccc ttcggggaca 1020 tggatacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080 gcaacgagcg caacccttat tatcagttgc cagcattaag ttgggcactc tggtgagact 1140 gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1200 gggctacaca cgtgctacaa tggatggtac aacgagttgc gaactcgcga gagtaagcta 1260 atctcttaaa gccattctca gttcggattg taggctgcaa ctcgcctaca tgaagtcgga 1320 atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc tgtacacacc 1380 gcccgtcaca ccatgagagt ttgtaacacc caaagtcggt ggggaacctt tagaaccgcc 1440 gctaaggcga tgg 1453

Claims (7)

A brewery-derived Lactobacillus plantarum GFC_B001 strain (accession number: KCTC 14244BP) having aldehyde dehydrogenase activity, acetaldehyde decomposition effect, and liver function protection activity against alcohol.


delete A probiotics preparation comprising at least one active ingredient selected from the group consisting of the strain of claim 1, a lysate of the strain, an extract of the strain, a culture solution of the strain, a concentrate of the culture solution, and a dried product.
The probiotics preparation according to claim 3, wherein the strain is freeze-dried and prepared in the form of live cells.
The probiotics formulation according to claim 3, wherein the probiotics formulation is any one formulation selected from the group consisting of liquid, powder, granule, tablet and capsule.
A food composition for relieving hangover and improving alcoholic liver damage comprising the probiotics preparation of claim 3.
The method of claim 6, wherein the food composition is for relieving hangover and improving alcoholic liver damage, characterized in that it is included in any one product selected from the group consisting of food, food additives, beverages, beverage additives, fermented milk and health functional foods. food composition.