KR20080064297A - Fungus making process for mold prevent of plant - Google Patents

Abstract

A method for preparing a strain is provided to obtain Bacillus subtilis PJFA(deposition no. KACC 91197P) which has antagonistic activity on Cylindrocarpon destructans and Rhizoctonia solani and is able to grow under the temperature at which ginseng grows. A method for preparing a strain for preventing fungus of a plant comprises the steps of: (a) diluting 1ml of suspension obtained suspending 1g of soil in 10ml of sterile saline with 9ml of sterile saline to obtain a diluted solution and repeating dilution to obtain 100mul of a diluted solution; (b) after smearing a PDA plate, which consists of 4g of a potato extract, 20g of glucose and 15g/liter of agar, has a pH of 5.1 and is smeared with spores of Rhizoctonia solani, with the 100mul of the diluted solution, culturing it at a temperature of 30 deg.C for 5 days; (c) after selecting microorganism forming clear zone from a culture material obtained from the step(b) and inoculating the selected microorganism into a PDA plate and a malt extract plate which consists of 20g of a malt extract, 20g of glucose, 1g of peptone, and 20g/liter of agar, respectively, culturing it at a temperature of 20 deg.C for 14 days.

Description

Manufacturing method of fungi for controlling fungi of fungi {Fungus making process for mold prevent of plant}

1 is Bacillus subtilis PJFA 16S rDNA lineage analysis

2 is Bacillus subtilis PJFA 16S rDNA Sequence

3 is Bacillus Antifungal Characterization of subtilis PJFA

4 is Bacillus another photo of antifungal characterization test of subtilis PJFA

5 is Bacillus Growth of Subtilis PJFA by Starch Concentration

6 is Bacillus Growth of Subtilis PJFA by Yeast Extract Concentration

7 is Bacillus Hourly Growth of subtilis PJFA

In general, plant diseases are caused by phytopathogenic fungi, and environmental pollution and drug-resistant strains are increasing, especially due to chemical fertilizer or pesticide abuse.

In order to control the disease caused by the fungus can be divided into physical methods to improve the cultivation environment, chemical methods using fungicides and biological methods using microorganisms.

However, the physical method has a small control effect and is difficult to apply equally to a large area, and the chemical method is less effective due to the emergence of drug resistant strains due to the use of fungicides. As an alternative method, researches on biological methods are being actively conducted, and many researches on microbial pesticides for eco-friendly agriculture are conducted.

Biological methods are methods that control pathogens and weeds that are harmful to plants by using antagonism or parasitic relationships among living organisms. The organisms, metabolites, or antibiotics of microorganisms that have symbiotic or dependent relationships with plants and promote plant growth. Substances are used.

Ginseng (Panax ginseng) is a perennial semi-negative herb that belongs to Ogapiaceae. The growth temperature is around 20 ℃ and above 30 ℃, the growth disorder is intensively grown in April ~ June according to these characteristics, and this time is the most vulnerable to pathogens.

In addition, ginseng is cultivated in one place for 4-6 years, so it is a crop that is very likely to interfere in the series.

Conventional control methods for such diseases are difficult to control against soil infectious pathogens, and problems related to pesticide residues of ginseng have already been raised in relation to the ginseng industry law, and thus, restrictions on use of pesticides to suppress ginseng diseases are further strengthened. It is a situation.

Due to the nature of ginseng cultivation, it is grown at various temperatures, and thus pathogens are also affected by various diseases throughout the year.

Therefore, a biological method that is persistent in ginseng pathogens and does not cause problems such as pesticide residue and environmental pollution is the most appropriate method, and the research and development of this is urgently needed.

The technical problem to be achieved by the present invention is to identify new microorganisms having antifungal properties and to establish the optimum culture conditions thereof, the cultured strain can be used as a raw material of functional by-product fertilizer, compost and pesticides.

Details of the present invention will be described with reference to the following examples, but the present invention is not limited by the following examples.

Example 1 Isolation and Identification of Antifungal Activity Strains

The microorganism of the present invention isolates the microorganisms with mycelial growth inhibitory effect of the root and ginseng root disease in the soil.

Identified Bacillus subtilis was deposited with the Institute of Agricultural Biotechnology (KACC), with the accession number KACC 91197P.

After inoculation with fungi and isolates and incubated for 3-4 weeks in the incubator at 20 ~ 25 ℃, the distance between the end of mycelial growth and the end of the growth of the isolated bacteria as mycelial growth was measured.

Experimental results showed that starch and maltose, xylose, glucose and mannitol were excellent as carbon sources, and yeast extract was the best as nitrogen source.

When the concentration of starch with the highest growth of carbon source was varied from 0% to 2%, the growth was the best at 2%, but the cell growth per starch concentration was the highest at 0.8%.

When the concentration of yeast extract, which was the best growth among nitrogen sources, was varied from 0 to 2%, the growth was the best at 2%, but the cell growth rate per unit concentration of yeast extract was the best at 1%.

The present invention provides a new microorganism having antifungal properties by providing Bacillus subtilis PJFA KACC 91197P, which is capable of growing at various temperatures during growth inhibition and ginseng growth and growing in spores and low nutrients. After identifying and establishing the optimum culture conditions, it is a beneficial invention that can be used as a raw material of functional by-product fertilizer, compost and pesticides based on this strain.

Claims (1)

A method for producing a fungus for controlling a fungus of a plant, which is prepared to be active while simultaneously inhibiting ginseng diarrhea and root ginseng.

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