CN110423707B - Bacillus subtilis strain NH-6 and application thereof - Google Patents
Bacillus subtilis strain NH-6 and application thereof Download PDFInfo
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- 244000063299 Bacillus subtilis Species 0.000 title claims abstract description 42
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- 241000243785 Meloidogyne javanica Species 0.000 claims abstract description 12
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- 241000813090 Rhizoctonia solani Species 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 7
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- 238000009472 formulation Methods 0.000 claims 3
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- 240000003768 Solanum lycopersicum Species 0.000 claims 1
- 244000038559 crop plants Species 0.000 claims 1
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- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 5
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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Abstract
The invention relates to the field of bacillus, in particular to a bacillus subtilis strain NH-6 and application thereof. A Bacillus subtilis strain NH-6 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC 17815. The bacillus subtilis strain NH-6 has the advantages of simple culture method, high growth speed, wide antibacterial spectrum, strong stress resistance and the like. The biological control preparation prepared from the bacillus subtilis strain NH-6 can be used as a biological pesticide or a biological fertilizer for controlling root-knot nematode diseases and various plant pathogenic fungi including black spot pathogen, rhizoctonia solani, gray mold pathogen and the like.
Description
Technical Field
The invention relates to the field of bacillus, in particular to a bacillus subtilis strain NH-6 and application thereof.
Background
The bacillus subtilis is one of bacillus, is widely distributed in soil and putrefactive organic matters, is easy to reproduce in the bacillus subtilis extract, and is widely applied to feeds and fertilizers and used as a bactericide. The preparation is a non-toxic, residue-free and pollution-free green microbial inoculum, has a wide development prospect, and shows great social and ecological benefits.
However, most of the existing bacillus subtilis only can singly play a role in controlling certain plant diseases, and some bacillus subtilis can control various plant diseases, but the effect is not ideal. Therefore, the invention is continued to invent a new bacillus subtilis capable of efficiently preventing and treating various plant diseases.
Disclosure of Invention
The invention aims to provide a novel bacterium, namely a bacillus subtilis strain NH-6, for efficiently preventing and treating various plant diseases and root-knot nematode diseases.
The bacillus subtilis strain NH-6 provided by the invention is obtained by separating and screening from soil, is preserved in China general microbiological culture Collection center in 2019, 5 months and 27 days, and has the specific addresses as follows: the Beijing, Chaoyang, Beichen Xilu No. 1 Hospital No. 3 has a preservation number of CGMCC 17815, and has the following morphological characteristics:
the gram stain of the bacillus subtilis strain NH-6 is positive, the thallus is rod-shaped, the PYS culture medium is cultured for 24 hours at 26 ℃ to generate spores, and the spores are oval and neutral. The bacterial colony at the initial culture stage is circular and milky, the edge is neat, the bacterial colony is raised into a steamed bun shape, and the surface is wet; the bacterial colony in the later culture period is light yellow, the edge is dentiform, and the surface is dried and wrinkled. When the strain is statically cultured in a liquid culture medium, the strain forms a white mycoderm on the surface of the culture medium, and the strain is combined with 'handbook for identifying common bacteria system', and the strain is bacillus.
The conventional culture method or propagation method of the bacillus subtilis strain NH-6 comprises the following steps:
inoculating the bacillus subtilis strain NH-6 into a PBGA culture medium for conventional culture at 25-30 ℃, wherein the culture medium formula comprises tryptone, beef extract, glucose and agar.
The second aspect of the invention discloses the application of bacillus subtilis strain NH-6 in the prevention and treatment of root-knot nematodes and the prevention and treatment of plant pathogenic fungi. The plant pathogenic fungi include black spot pathogen, Rhizoctonia solani and Botrytis cinerea.
It is to be understood that the present invention is not limited to root-knot nematodes, black spot, sheath blight and gray mold, and that any species or bacteria capable of being controlled by the Bacillus subtilis strain NH-6 of the present invention are within the scope of the present invention.
In a third aspect, the invention discloses a biological control preparation, which comprises the bacillus subtilis strain NH-6.
The fourth aspect of the invention discloses a method for preparing the biological control preparation, which comprises the following steps:
s1: preparing seed solution of bacillus subtilis strain NH-6;
s2: inoculating the seed liquid obtained in the step S1 into a culture medium for fermentation culture to obtain the biological control preparation.
In a fifth aspect of the present invention, there is disclosed a method for controlling root knot nematode disease in crops which comprises applying the above-mentioned Bacillus subtilis strain NH-6 or the above-mentioned biological control agent to crops having root knot nematodes.
Preferably, the crop is tomato.
It is to be understood that the scope of the present invention is not limited to tomatoes, and that other suitable crops can be used by those skilled in the art and are within the scope of the present invention, given the teachings of the present invention.
Compared with the prior art, the invention has the following advantages or beneficial effects:
the bacillus subtilis strain NH-6 has the advantages of simple culture method, high growth speed, wide antibacterial spectrum, strong stress resistance and the like. The biological control preparation prepared from the bacillus subtilis strain NH-6 can be used as a biological pesticide or a biological fertilizer for controlling root-knot nematode diseases and various plant pathogenic fungi including black spot pathogen, rhizoctonia solani, botrytis cinerea and the like.
Drawings
The invention is further illustrated by means of the attached drawings, the examples of which are not to be construed as limiting the invention in any way.
FIG. 1 is a schematic diagram showing the antagonistic action of Bacillus subtilis strain NH-6 against Rhizoctonia solani in the examples of the present invention;
FIG. 2 is a schematic diagram showing the antagonistic action of Bacillus subtilis strain NH-6 on Sphaerotheca fuliginea in the example of the present invention;
FIG. 3 is a schematic diagram showing the antagonistic action of Bacillus subtilis strain NH-6 on Botrytis cinerea in the present invention;
FIG. 4 is a schematic diagram showing that Bacillus subtilis NH-6 has an antagonistic effect on the growth of Meloidogyne incognita.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are only for the purpose of the present invention and are not intended to limit the scope of the present invention.
Example 1 isolation and purification of Bacillus subtilis Strain NH-6
The bacillus subtilis strain NH-6 in the embodiment is obtained by separating soil by a dilution plate method and a plate marking method, and the separation method comprises the following steps: in a tomato plant with root-knot nematode, healthy roots are selected to collect soil, the soil is dissolved by sterile water, supernatant is sequentially diluted and coated on a flat plate, a single colony is picked for slant culture, and primary screening, secondary screening and quantitative screening are carried out by taking rhizoctonia solani as indicator bacteria.
The separated bacillus subtilis strain NH-6 is positive in gram staining, the thallus is rod-shaped, and after being cultured for 24 hours at 26 ℃ in a PYS culture medium, spores are generated, wherein the spores are oval and are neutral. The bacterial colony is circular and milky white in the initial culture stage, the edge is neat, the bacterial colony bulges are in a steamed bun shape, and the surface is wet; the bacterial colony in the later culture period is light yellow, the edge is dentiform, and the surface is dried and wrinkled. When the strain is statically cultured in a liquid culture medium, the strain forms a white bacterial film on the surface of the culture medium, which is combined with the handbook for identifying common bacterial systems, and the strain is a bacillus. The specific physiological and biochemical characteristics of the separated bacillus subtilis strain NH-6 are shown in Table 1.
TABLE 1 physiological and biochemical Properties of NH-6
Among them, the catalase in Table 1 is specifically catalase.
The bacillus subtilis strain NH-6 is preserved in China general microbiological culture Collection center with the preservation number of CGMCC 17815.
Example 2 NH-6 Strain 16S rDNA identification
The DNA of the NH-6 strain extracted in the example 1 is subjected to PCR amplification, the cloning analysis of the amplified gene fragment, and the sequencing result shows that the length of the 16S rDNA sequence of the strain is l510bp, and the homology comparison of the sequencing result (GenBank accession number is DF520956) and the gene sequence of the Bacillus subtilis 16S rDNA in NCBI shows that the homology of the 16S rDNA sequence of the strain and the sequence of the Bacillus subtilis 16S rDNA reaches 99%. And preliminarily determining the NH-6 to be Bacillus subtilis by integrating the morphological characteristics of the NH-6 strain and the measurement results of physiological and biochemical indexes.
Example 3 NH-6 Strain having antagonistic action against various plant diseases
NH-6 strain is picked and transplanted to PBGA slant, and cultured for 36h at 26 ℃. The bacterial inhibition tests are carried out by taking rhizoctonia solani, black spot pathogen and gray mold pathogen as indicator bacteria, and the antagonism must be carried out under the condition of consistent environmental conditions. Transplanting pathogenic bacteria to be tested on an activated PDA (personal digital assistant) plate, culturing at 26 ℃ for 48h, punching on a bacterial-carrying PDA plate by using a puncher, connecting NH-6 strains to the bacterial-free PDA plate in a diagonal manner, transferring a bacterial block of the pathogenic bacteria to be tested in the center of the plate after growing for 10h, culturing at 26 ℃ for 48h, placing a pathogenic bacterial block but not connecting the NH-6 plate as a contrast, measuring the width of an antagonistic zone when the contrast is full of the plate, and repeatedly treating each pathogenic bacterial strain for 3 times. The results are shown in FIGS. 1-3, in which panel A is the control and panel B is the treatment. By analyzing and comparing the experimental results of fig. 1 to fig. 3, it can be concluded that the NH-6 strain has antagonistic action against various plant diseases.
Example 4 NH-6 strains have antagonistic effects on the growth of root-knot nematodes
And (4) after the tomato seedlings are transplanted for 3d, inoculating NH-6 strains. Randomly drawing the transplanted tomato seedlings, irrigating roots and inoculating 25mL of NH-6 bacterial suspension (1010 CFU/mL); after 3 days of root irrigation treatment, 5mL (90 strips/mL) of nematode suspension was diluted and instilled onto the tomato roots for direct inoculation of 5mL (90 strips/mL) of nematode suspension treatment as a control. NH-6 and clear water as treatment groups. Each treatment of 20 seedlings was repeated 3 times. Culturing at 26 deg.C and 50% relative humidity for 5 weeks. And (4) taking out the inoculated plants, counting the occurrence condition of diseases, and calculating the severity and prevention effect of the diseases. The results are shown in FIG. 4, in which A is a control group and B is a treatment group, from which it can be concluded that NH-6 strain has an antagonistic effect on the growth of root-knot nematodes.
The embodiments of the present invention have been described in detail, but the embodiments are only examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications and substitutions for the present invention are within the scope of the present invention for those skilled in the art. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (7)
1. A Bacillus subtilis strain NH-6 is preserved in China general microbiological culture Collection center with the preservation number of CGMCC 17815.
2. Use of the bacillus subtilis strain NH-6 of claim 1 for the control of meloidogyne.
3. Use of the bacillus subtilis strain NH-6 according to claim 1 for the control of phytopathogenic fungi;
the plant pathogenic fungi are black spot pathogen, rhizoctonia solani and gray mold pathogen.
4. A biocontrol formulation comprising bacillus subtilis strain NH-6 of claim 1.
5. A method of preparing the biocontrol formulation of claim 4, comprising the steps of:
s1: preparing seed solution of bacillus subtilis strain NH-6;
s2: inoculating the seed liquid obtained in the step S1 into a culture medium for fermentation culture to obtain the biological control preparation.
6. A method for controlling root knot nematode disease in crops comprising applying the bacillus subtilis strain NH-6 of claim 1 or the biological control formulation of claim 4 to crops having root knot nematodes in their roots.
7. The method of claim 6, wherein the crop plant is a tomato plant.
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| CN111302857B (en) * | 2020-02-26 | 2022-07-05 | 新疆农垦科学院 | Water-soluble organic biological bacterial fertilizer for drip irrigation tomatoes and preparation method thereof |
| CN112175050A (en) * | 2020-08-19 | 2021-01-05 | 南通大学 | Extracellular antibacterial protein of bacillus subtilis and application thereof |
| CN112226392A (en) * | 2020-11-10 | 2021-01-15 | 青岛滋百农农业科技有限公司 | Bacterial strain for preventing and treating ginger root-knot nematode disease and promoting ginger growth and application thereof |
| CN112831443B (en) * | 2021-02-22 | 2022-08-02 | 青岛农业大学 | Bacillus subtilis, microbial inoculum and application thereof in preventing and treating plant diseases |
| CN113151065B (en) * | 2021-03-30 | 2022-08-05 | 山东蔚蓝生物科技有限公司 | Bacillus subtilis for preventing and treating nematode diseases and application thereof |
| CN113880925B (en) * | 2021-11-05 | 2023-11-03 | 南通大学 | Extracellular antibacterial protein of bacillus subtilis and application thereof |
| CN115418333A (en) * | 2022-09-08 | 2022-12-02 | 河北省科学院生物研究所 | Bacillus subtilis ZLP-121 and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012087980A1 (en) * | 2010-12-21 | 2012-06-28 | Agraquest, Inc. | Sandpaper mutants of bacillus and methods of their use to enhance plant growth, promote plant health and control diseases and pests |
| CN103703120A (en) * | 2011-05-26 | 2014-04-02 | Sds生物技术株式会社 | Strain belonging to bacillus genus, microbiological agent, and plant cultivation method |
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| US7033781B1 (en) * | 1999-09-29 | 2006-04-25 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2012087980A1 (en) * | 2010-12-21 | 2012-06-28 | Agraquest, Inc. | Sandpaper mutants of bacillus and methods of their use to enhance plant growth, promote plant health and control diseases and pests |
| CN103703120A (en) * | 2011-05-26 | 2014-04-02 | Sds生物技术株式会社 | Strain belonging to bacillus genus, microbiological agent, and plant cultivation method |
Non-Patent Citations (2)
| Title |
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| Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway;Verónica Donato et al;《Nat Commun》;20170130;第8卷;全文 * |
| 枯草芽孢杆菌菌株RSS_1对油菜菌核病菌的生防作用及其机制研究;代玉立;《中国优秀博士学位论文全文数据库 农业科技辑》;20170215;第119页第2节，表7-1至7-2，图7-1至7-4 * |
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