KR20080051523A - Food additive for preventing specializing fat cell of infant - Google Patents

Abstract

A food additive for inhibition of adipocyte differentiation of infants containing fucoidan, fucose and galactose is provided to be effective in development of a brain cell and nerve cell while inhibiting adipocyte differentiation in infants. A food additive for inhibition of adipocyte differentiation of infants contains 55 to 90% by weight of fucoidan extracted from sporophyll of sea mustard, sea tangle, or Rheum Root, 5 to 25% by weight of fucose and 5 to 20% by weight of galactose in a powder form. The food additive for inhibition of adipocyte differentiation of infants also contains 100 parts by weight of the food additive for infant, 250 to 500% by weight of mannitol from seaweed and 100 to 150% by weight of soybean protein.

Description

Food additive for preventing specializing fat cell of infant

1a to 1e is a cell photograph for explaining the effect of inhibiting the adipocyte differentiation of food additives for inhibiting adipocyte differentiation of infants, and

Figure 2 is a graph showing the results of measuring the amount of fat actually produced by staining fat globules.

The present invention relates to food additives, and more particularly, to food additives for infants, which are easy to absorb and inhibit the adipocyte differentiation of infants and are effective in the development of brain cells and nerve cells.

Childhood obesity is a social problem worldwide because it causes a variety of diseases. In the United States, about 20% of the pediatric population is classified as childhood obesity, and in Korea, it is estimated that 10 to 15% are childhood obesity. However, childhood obesity and adult obesity occur in different forms. Since childhood obesity occurs during the rapid growth period, the number of adipocytes increases, and fat is saturated in the adipocytes of adult obesity. As a result, the differentiation of adipocytes increases during the early childhood, which is a rapid growth period, and the number of fat cells increases, increasing the likelihood of obesity in adults. In fact, more than 80% of childhood obesity patients are reported to progress to adult obesity.

Childhood obesity is a social problem because diabetes, hyperlipidemia, hypertension, and heart disease, known as adult diseases, occur in children with childhood obesity. Therefore, suppressing the differentiation of fat cells during infancy is very important to maintain healthy life. To date, obesity inhibitors are known as materials containing dietary fiber, materials that inhibit adipocyte differentiation, materials that promote fat metabolism, and the like. It is known that each material has a function of suppressing obesity, but the range of use is limited due to inconveniences such as stomach and intestines or safety issues. Therefore, the development of fat cell differentiation inhibiting material proved to be safe is very important.

On the other hand, fucoidan is a substance contained in brown algae, such as kelp, seaweed, and rhubarb, and its structure is a highly complex sulfated polysaccharide mainly composed of esterified sulfuric acid in L-fucose. The various physiological functionalities of fucoidan are believed to be closely correlated with the composition and structure of the fucoidan, and the functions of fucoidan, reported in the academic world, include immunopotentiation, hypoglycemic activity, antioxidant activity, and triglycerides. Inhibitory effect, anticoagulant action, gastric ulcer healing action, anti-tumor action, liver function enhancement action, apotosis action, anti-allergic action, neovascular control action, intestinal action, cholesterol lowering action, HGF production induction action, blood pressure Synergistic inhibitory action, IgE overproduction inhibitory action, and antibacterial action of H. pylori . Fucoidan has a low viscosity and excellent solubility compared to other polysaccharides, and has a high value as an additive for preventing viral diseases of fish and animals including basic raw materials and additives. However, a large amount of iodine is extracted along with fucoidan from kelp, and excessive intake of iodine has side effects such as thyroid disorders. In addition, milk contains a large amount of galactose, which is known to be good for the development of brain cells and nerve cells as a sugar, but infants and toddlers are not digested when ingested, so milk powder containing galactose is commercially available. There is a problem that it is difficult to feed a large amount of powdered milk to provide galactose to them.

The present invention has been made in order to solve the above problems, the technical problem to be achieved by the present invention is not only inhibit the differentiation of fat cells in the growth phase, including fucoidan extracted from seaweed spores, kelp, or rhubarb brain of infants It is to provide an optimized food additive for infants and toddlers comprising galactose in an easily absorbable form effective for the development of cells and nerve cells.

Infant food additives according to the present invention for achieving the above technical problem is 55 to 90% by weight of fucoidan, 5 to 25% by weight of fucose and 5 to 20% by weight of galactose as a component extracted from the spores, kelp, or rhubarb of seaweed It is characterized by including the percentage in the powder form.

In addition, it is preferable to further include 250 to 500% by weight of seaweed mannitol, 100 to 150% by weight of soy protein based on 100% by weight of the baby food additive.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Infant food additives according to the present invention as a component extracted from seaweed spores, kelp, or rhubarb,

Fucoidan 55-90 weight percent,

Fucose 5-25 weight percent, and

Galactose 5 to 20% by weight characterized in that it comprises a powder form.

First, anti-adipocyte differentiation effect was evaluated for the above food additives. Describe the materials and methods. First, 3T3-L1 preadipocytes (predifferentiated cells) were cultured using DMEM (Dubelcco's Modified Eagles' Medium) containing 10% FBS (fetal bovine serum) as a cell culture process. Adipocyte differentiation was induced by adding 1 μM dexamethasone, 10 μg / ml insulin and 0.5 mM isobutylxanthine to the cultured cells, followed by incubation for 2 days. 250 μM of theophylin, a drug used previously, was administered to induced adipocytes and used as a positive control group, and fucoidan (0.1 mg / ml and 0.2 mg / ml) was added to evaluate the inhibitory effect of adipocyte differentiation. It was.

Next, as a process for staining fat, 10% neutral formalin (10% buffered neutral formalin) was added to the cells, and the cells were fixed and washed with 60% propanol. After washing, 1 ml of 0.3% Red O staining reagent was added to stain the fat globules, and the absorbance was measured at 510 nm using an ELISA reader.

1A to 1E are enlarged photographs of cell tissues as experimental results. The cells inducing adipocyte differentiation (FIG. 1B) can be observed to rapidly increase the differentiation of adipocytes as compared to the control group (FIG. 1A) not induced. In the case of administration of theophyline (C), which is known to inhibit adipocyte differentiation, adipocyte differentiation was significantly inhibited compared to adipocyte induction group (B). Groups (D, E) administered fucoidan can be seen that the differentiation of adipocytes is suppressed when visually observed.

To evaluate the stomach objectively, the effect of inhibiting adipocyte differentiation by oil red O staining and sensory evaluation of 100 infants were evaluated. A, B, and C represent the composition ratios of fucoidan, fucose, and galactose, respectively, in the oil red O staining evaluation results, Δ is less than 10%, and ○ is 10 to 15%. , ◎ indicates a difference in dyeing degree of more than 20%, ◎ is tasteless and odorless in sensory evaluation, ○ has no difficulty in ingestion, ○ has a slight taste change but no difficulty in ingestion, × indicates a taste change in sensitive infants with 5% or more When sensing and refusal to consume occurred, the following results were obtained.

Comparative Example 1 A: 45 B: 30 C: 25 First Embodiment A: 55 B: 25 C: 20 Second Embodiment A: 75 B: 15 C: 10 Third Embodiment A: 90 B: 5 C: 5 Comparative Example 2 A: 95 B: 2.5 C: 2.5 Adipocyte differentiation inhibitory effect  △  ○  ◎  ◎  ◎ Sensory evaluation results ◎ ◎ ◎ ○ ×

Referring to Table 1, in Example 1, oil red O staining was evaluated for cells inducing adipocyte differentiation by adding 55% by weight of fucoidan, 25% by weight of fucose, and 20% by weight of galactose. In the evaluation of the effect of inhibiting the differentiation of adipocytes, the staining difference was more than 10-15% compared to that of the control group, and the effect of the inhibition of adipocyte differentiation was somewhat significant.

In the second embodiment, 75% by weight of fucoidan, 15% by weight of fucose, and 10% by weight of galactose were added to the cells that induced adipocyte differentiation. In the evaluation, the difference of staining degree was more than 20% compared to the control group, indicating that the effect of inhibiting fat cell differentiation was significant.

In Example 3, the effect of inhibiting fat cell differentiation through oil red O staining was evaluated for cells inducing fat cell differentiation by adding 90% by weight of fucoidan, 5% by weight of fucose, and 5% by weight of galactose. In the evaluation, the difference in staining degree was more than 20% compared to the control group, indicating that the effect of inhibiting fat cell differentiation was significant and there was a slight taste change in sensory evaluation, but there was no difficulty in ingestion.

On the contrary, in Comparative Example 1, 45% by weight of fucoidan, 30% by weight of fucose, and 25% by weight of galactose were added to inhibit the fat cell differentiation by assessing the oil red O staining level for the cells inducing the adipocyte differentiation. In the effect evaluation, the difference in staining degree within 10% compared to the control group was not significant. Also, in Comparative Example 2, 95% by weight of fucoidan, 2.5% by weight of fucose, and 2.5% by weight of galactose were added to inhibit the fat cell differentiation by assessing the oil red O staining rate on the cells inducing adipocyte differentiation. In the effect evaluation, the staining difference was more than 20% compared to the control group, and there was an effect of inhibiting the adipocyte differentiation, but in sensory evaluation, it was difficult to commercialize because it detected the taste change and rejected the intake in sensitive infants of 5% or more.

As described above, since childhood obesity occurs during the rapid growth phase, the number of adipocytes is increased, and the fat is saturated in the fat cells of adult obesity, thereby increasing the differentiation of adipocytes during the rapid growth phase. Increasing the number increases the likelihood of obesity in adults, so suppressing the differentiation of fat cells in infants and toddlers will be very important to maintain healthy life.

Here, while the known efficacy of fucoidan is neutral fat suppression or diet effect to reduce the already produced fat cells, the benefits of fucoidan in food additives according to the present invention to feed infants and young children in the growth phase by inhibiting the differentiation of fat cells It should be noted that by preventing the differentiation of mast cells themselves, it is possible to prevent obesity very differently from the conventional method, which can greatly reduce the possibility of becoming obese in adulthood. In other words, the food additive for inhibiting adipocyte differentiation of infants and toddlers can prevent adult obesity, which causes adult disease, when they become adults by inhibiting adipocyte differentiation.

However, in case of fucoidan extracted from seaweed spores, kelp, or other parts of seaweed other than rhubarb, or other brown algae, it had some effect on inhibiting the differentiation of adipocytes, but it was necessary to increase the dose to make the peculiar taste of brown seaweed unique. Infants were reluctant to eat, resulting in unsatisfactory results in sensory evaluation.

In addition, the food additive for inhibiting the differentiation of fat cells of infants and toddlers includes fucoidan extracted from Wakame spores, kelp, or rhubarb in powder form. It is known to help development. Milk contains a large amount of galactose, but the galactose is lactose and people with some lactose intolerance have problems with digestive disorders if the enzymes are not supplied properly. Some people have an allergic reaction to milk proteins. On the other hand, according to the present invention, the galactose constituting the fucoidan is not a lactose but is hydrolyzed as a polysaccharide and absorbed into the body, and there is no big problem. It is possible. That is, the food additive for inhibiting adipocyte differentiation of infants and toddlers includes galactose, which is effective for the development of brain cells and nerve cells, in an easily absorbed form.

Figure 2 shows the results of measuring the amount of fat actually produced by staining the fat globules. Referring to FIG. 2, there was a tendency to decrease in the case of theophyline, which is known to inhibit adipocyte differentiation, but there was no significant difference. However, the fucoidan group significantly inhibited the production of fat. These results indicate that the administration of fucoidan can inhibit adipocyte differentiation.

On the other hand, as another embodiment of the food additive for suppressing the differentiation of fat cells of infants and toddlers according to the present invention will be described an example of adding seaweed mannitol and soy protein in consideration of taste and nutrients.

Raw material  persons 1 serving  Packing unit Fucoidan (Fucoidan) 200 ~ 2.000mg Seaweed Mannitol 500 ~ 5,000mg Soy Protein (ISP) 200 ~ 3,000mg Lactobacillus Appropriate amount Colostrum Powder Appropriate amount Calcium Appropriate amount Magnesium Appropriate amount Casein phosphopeptide (CPP) Appropriate amount Vitamin D ₃ Appropriate amount Folic acid Appropriate amount Other minerals, vitamins Appropriate amount

Fucoidan added amount should be taken into consideration the weight and absorption rate according to age and has a characteristic fishy taste so sensory evaluation is also necessary. Fucoidan is also a polysaccharide, so its effect on solubility and digestion should be considered. Therefore, it is preferable to add 0.2 g to 2 g depending on the age.

According to another embodiment of the present invention, with respect to 100 weight percent of baby food additives, the additive is composed of 250 to 500 weight percent of seaweed mannitol, and 100 to 150 weight percent of soy protein. Infants and baby food additives containing fucoidan have been stored at room temperature for about 30 days or more, the cause of which cannot be elucidated. However, when the algal mannitol and soy protein were mixed at a certain composition ratio, the effect of inhibiting fat cell differentiation was reduced to within 10% even when stored at room temperature for about 30 days or more. After oil storage at room temperature for 30 days, X indicates that the effect of inhibiting adipocyte differentiation was reduced by more than 50%, △ decreased by 10% to 40%, and ○ decreased within 10%. No difficulty at all, × was the result of indigestion symptoms in more than 5% sensitive infants, the following results were obtained.

Comparative Example 1 D: 100 E: 50 Comparative Example 2 D: 220 E: 80 First Embodiment D: 250 E: 100 Second Embodiment D: 400 E: 120 Third Embodiment D: 500 E: 150 Comparative Example 3 D: 600 E: 200 Change over time (inhibition of fat cell differentiation)  ×  △  ○  ○  ○  ○ Digestive Absorption Assessment ○ ○ ○ ○ ○ ×

Referring to Table 3, in the first embodiment, after mixing 250% by weight of seaweed mannitol and 100% by weight of soy protein with respect to 100% by weight of the baby food additive described with reference to Table 1, while adding at room temperature for 30 days In the evaluation of the effect of inhibiting the adipocyte differentiation through the oil red O staining evaluation, the cells induced the adipocyte differentiation were reduced by less than 10%, and the suppression of the change over time was good. In Example 2 and Example 3, 400 and 500 weight percent of seaweed mannitol and 120 and 150 weight percent of soy protein were mixed and allowed to stand for 30 days at room temperature, and then added to cells inducing adipocyte differentiation. In the evaluation of the effect of inhibiting the differentiation of fat cells through oil red O staining, it was shown that the effect of preventing the change over time was reduced by only 10%.

On the contrary, in Comparative Example 1, 100% by weight of seaweed mannitol and 50% by weight of soy protein were mixed, and then allowed to stand for 30 days at room temperature and then added to the cells to induce adipocyte differentiation. In the evaluation of the differentiation inhibitory effect, it was significantly reduced by more than 50% compared to the case of no addition. In Comparative Example 2, the result of the above evaluation for the seaweed mannitol 220% by weight and the soy protein 80% by weight was reduced by about 25%. appear. In Comparative Example 3, 600 wt% of seaweed mannitol and 200 wt% of soy protein were reduced by less than 10%, but symptoms of indigestion appeared in 5% or more sensitive infants.

In addition, in addition to the novel effects of inhibiting the changes over time by mixing seaweed mannitol and soy protein in fucoidan, seaweed mannitol has been reported to reduce edema, weight loss, etc. as a low calorie sweetener. In addition, there is a cool sweet taste can have a big impact on the taste of the product. In consideration of age and weight, it is preferable to add 0.5 to 5 g.

Soybeans are known for their high protein content and are well known as a source of vegetable protein. Although soy protein is a vegetable protein, it has a good content and composition of essential amino acids, so it can be used as a good protein source for growing children. Soy protein is a sulfur-containing amino acid methionine (methionine) is the first restriction amino acid, but the quality of protein can be improved by the addition of colostrum powder, CPP and the like. Soy protein has a disadvantage of poor solubility because it has a very high molecular weight. Therefore, the addition amount per bag was set to 200 ~ 3,000mg based on the solubility and sensory test. Although the above is described as the amount of packaging per package, it is equivalent to 250 to 500% by weight of seaweed mannitol and 100 to 150% by weight of soy protein for 100% by weight of baby food additives.

Although milk is relatively rich in protein, it is difficult to digest in infants, but if you use 100% to 150% by weight of soy protein, you will be able to consume protein without any difficulty in digestion.

On the other hand, lactic acid bacteria are known as a material that can improve the intestinal environment by inhibiting the growth of harmful bacteria. Especially for children in the growing season, it is very important to improve the intestinal microbial environment. Therefore, in consideration of age and body weight, based on 100% by weight of the baby food additive according to an embodiment of the present invention, to add 10 to 20% by weight.

Colostrum powder, CPP (casein phosphopeptide) added to the product is preferably added to 5 to 15% by weight in consideration of the synergistic effect of proteins, promoting the absorption of calcium.

In addition, it is desirable to add 2 to 5 wt% of minerals and vitamins based on the 8th Dietary Reference Intakes for Koreans (KDRIS, Korean Nutrition Society, Korean Nutrition Commission Committee, 2005). Do.

According to the present invention, a food additive optimized for infants and toddlers including galactose, which is effective in the development of brain cells and nerve cells, as well as inhibiting the differentiation of fat cells of infants in the growing phase, is provided.

In addition, after mixing algae mannitol and soy protein at a predetermined ratio, the cells were left at room temperature for about 30 days and evaluated for suppressing the effect of adipocyte differentiation on the cells inducing adipocyte differentiation. It also has the effect of easy storage.

As described above, according to the present invention, food additives optimized for infants and toddlers, including galactose, which are effective in the development of brain cells and nerve cells, as well as inhibiting the differentiation of fat cells of infants in the growing phase, are easily absorbed. This is provided. In addition, there is an effect that not only replenishment of nutritional ingredients but also easy storage.

Claims (2)

Adipocyte differentiation of infants and toddlers, comprising 55 to 90% by weight of fucoidan, 5 to 25% by weight of fucose, and 5 to 20% by weight of galactose as extracts from the seaweed spores, kelp, or rhubarb Inhibitory food additives. The method of claim 1, For 100% by weight of the baby food additive, Food additives for inhibiting adipocyte differentiation of infants and infants, characterized in that it further comprises 250 to 500% by weight of seaweed mannitol, 100 to 150% by weight of soy protein.

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