KR20080012546A - Dodok (codonopsis lanceolata) extracts, funtional beverage comprising dodok (codonopsis lanceolata) extracts as main components and method of preparing the same - Google Patents

Abstract

A method of manufacturing a functional beverage containing Codonopsis root extract as an active ingredient is provided to obtain the beverage effective for treatment and prevention of cancer and hypertension and easy to drink. An anticancer and antihypertensive beverage contains 0.01 to 0.1% by weight of Codonopsis root extract and additives containing 1 to 5% by weight of sugars, 0.1 to 0.5% by weight of citric acid, 0.01 to 0.005% by weight of vitamin C and 0.1 to 0.5% by weight of dextrin. The Codonopsis root extract is obtained by extracting Codonopsis root with water, alcohol or enzyme. The water-extract of Codonopsis root is obtained by the steps of: adding Codonopsis root with 2 to 4 times their weight of water; adding 0.5 to 0.9% by weight of enzyme thereto to adjust the pH; extracting at 36 to 38deg.C for 4 to 6hr in an incubator; and freeze-drying to 70 to 90meshes. The alcohol is 30 to 60% ethanol. The enzyme is cellulase.

Description

Deodek extract, functional beverage using the same as an active ingredient, and a method for producing the same

Figure 1 shows the measurement of cancer cell proliferation inhibitory effect according to the concentration of 1, 3, 5, 7, 9, 12mg / ml of the extract of the present invention prepared according to Examples 1 to 5,

Figure 2 shows the results for the antihypertensive effect of the extract of Sandodeok of the present invention prepared according to Examples 1 to 5,

3 is a manufacturing process of the sand extract extract and beverage according to an embodiment of the present invention,

Figure 4 is a manufacturing process of the sand duck drink according to an embodiment of the present invention.

The present invention relates to a deodeok extract showing an anticancer effect and an antihypertensive effect, a beverage using the same as an active ingredient, and a method for preparing the same.

Deodeok is a perennial vine plant belonging to Campahutaceae, and its cultivation history is not long, and it has been harvested mainly in the wild, and has been grown for the sake of herbs and edibles. Deodeok is known to be effective in tonsillitis, ulcers, pulmonary tuberculosis, asthma, detoxification, expectoration and cough, and has been widely used as a ginseng substitute for ginseng. Codonopsis lanceolata Benth et Hook) includes wild ducks and wild cultivators, which are superior to the ones in taste and aroma, but there are no objective data.

Sandodeok is a perennial herb that is wild in the mountainous regions of Korea, China, and Japan. Since ancient times, it has been widely used as a folk medicine for ginseng as a common food and ginseng. Due to its unique flavor, sanda duck is widely used for raw foods such as baked and pickled foods, and is used as a raw material for some processed foods. However, its use is still very limited compared to potential use value. Therefore, it is not only academic, technical, but economical to develop high value-added food ingredients and processed foods by demonstrating the scientific excellence of Sandodeok by scientifically exploring the various medicinal effects as well as the new physiological functions. In that sense, the significance is very large.

In particular, hypertension, cancer, etc., which occupy most of modern adult diseases, are diseases that all modern people have to overcome, and these diseases are derived from eating habits to improve their eating habits or to take long-term use of functional foods that are effective for these diseases. I would say. In particular, the drink is easy and easy, and excellent taste, it would be more desirable if the drink does not have a refusal to drink. The inventors of the present invention have completed the present invention while studying to complete a functional food having excellent anti-cancer and antihypertensive properties, in particular, a functional beverage that is easy to drink.

An object of the present invention is to provide a deodeok extract effective for the prevention and treatment of anticancer and antihypertension, a functional health drink effective for the prevention and treatment of antihypertension and anticancer using the same, and a method for producing the same.

In order to achieve the object of the present invention,

Deodeok extract, which is effective for the prevention and treatment of anticancer and antihypertension obtained by extracting Deodeok with water, alcohol, or enzyme.

The deodeok extract extracted with water may be a hydrothermal extract obtained by extracting and filtering Deodeok by adding water 2 to 4 times the weight of Deodeok for 3-6 hours at 90 ~ 150 ℃.

Deodeok extract extracted with the alcohol may be an alcohol extract obtained by extracting and filtering the Deodeok 2 ~ 4 times the weight of the deodeok for 3-6 hours at 90 ~ 150 ℃ and filtered. Herein, the alcohol may be preferably used in one embodiment of the present invention. The alcohol is preferably 30 to 60% alcohol.

Deodeok extract extracted with the enzyme was added to 2 ~ 4 times the weight of the deodeok to the weight of the deodeok 0.5 ~ 0.9% of the enzyme is added to adjust the pH to 4 ~ 6 and then 4 ~ 6 in an incubator at 36 ~ 38 ℃ After extracting for time may be an enzyme extract obtained by filtration with filter paper. Herein, the enzyme may be a cellulase.

The deodeok is preferably extracted after grinding using a grinder to improve the extraction yield of the deodeok extract.

    The deodeok extracts may be frozen and powdered with a freeze dryer, and then filtered by 70 to 90 mesh.

The deodeok may be a sand dude or a field deodeok.

For another object of the present invention, the present invention

     It provides a deodeok functional health drink effective in anti-cancer and antihypertensive containing 0.01 ~ 0.1% by weight of the deodeok extract as an active ingredient. If the deodeok extract is less than 0.01% by weight based on the total weight of the Deodeok beverage, you can not feel the aroma and taste of the deodeok at all, and if the deodeok extract is more than 0.1% by weight, the aroma and bitter taste of the deodeok is too strong to reduce the preference There is. In terms of pharmacological effects, the higher the concentration, the more effective it is possible to add more if there is no problem in drinking, and even at low concentrations, since the useful ingredients can accumulate in the body due to continuous drinking, the function can be exerted. Although the above-mentioned effective range is preferable, it may be used outside the range in view of efficacy.

The deodeok extract is preferably a hot water extract.

The deodeok extract can be used as a main ingredient of the deodeok beverage by concentrating the extract to up to 60 Brix (Brix) to increase the shelf life.

The deodeok functional health drink may further include 1 to 5% by weight of sugar, 0.1 to 0.5% by weight of citric acid, 0.01 to 0.005% by weight of vitamin C, 0.1 to 0.5% by weight of dextrin.

For another object of the present invention

Grinding the deodeok with a grinder,

Extracting the pulverized deodeok with water, alcohol or enzyme,

Provides a method for producing a deodeok functional health drink effective in anti-cancer and antihypertensive comprising the step of mixing purified water so as to include 0.01 ~ 0.1% by weight relative to the total beverage weight by using the deodeok extract as an active ingredient.

The manufacturing method of the deodeok functional health drink may further comprise the step of filtering the extract.

The deodeok extract extracted with water may be a hydrothermal extract obtained by extracting and filtering Deodeok by adding water 2 to 4 times the weight of Deodeok for 3-6 hours at 90 ~ 150 ℃.

Deodeok extract extracted with the alcohol may be an alcohol extract obtained by extracting and filtering the Deodeok 2 ~ 4 times the weight of the deodeok for 3-6 hours at 90 ~ 150 ℃ and filtered. Herein, the alcohol may be preferably used in one embodiment of the present invention. The alcohol is preferably 30 to 60% alcohol.

Deodeok extract extracted with the enzyme was added to 2 ~ 4 times the weight of the deodeok to the weight of the deodeok 0.5 ~ 0.9% of the enzyme is added to adjust the pH to 4 ~ 6 and then 4 ~ 6 in an incubator at 36 ~ 38 ℃ After extracting for time may be an enzyme extract obtained by filtration with filter paper. Herein, the enzyme may be a cellulase.

In addition, in order to improve the palatability and functionality of the deodeok beverage in the step of mixing the deodeok solution and purified water, a known food additive may be added as a subsidiary material.

The manufacturing method of the deodeok functional health drink, as other food additives, may include adding 1 to 5% by weight of sugar, 0.1 to 0.5% by weight citric acid, 0.01 to 0.005% by weight of vitamin C, 0.1 to 0.5% by weight of dextrin Can be.

On the other hand, the additive is a kind of food additives used in the beverage field, if one of ordinary skill in the art such additives can be selected and used appropriately, so the food additive in the deodeok beverage of the present invention is Not only the food additives but various food additives can be used according to the consumer's preference.

      Acid deodeok beverages according to an embodiment of the present invention is a raw material blending step as shown in Figure 4; Foreign material removal step; Preheating step; Degassing step; Sterilization step; Filling step; Sealing step; Cap sterilization step; It may consist of a cooling step and a packing step.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are described by way of example and should not be construed as limiting the scope of the invention.

Example  One: Sanderuck Hydrothermal  Preparation of Extract

1Kg of sandalwood was washed well to remove foreign substances, and then pulverized using a pulverizer, and extracted by adding three times the weight of sandalwood at 100 ° C for 4 hours, and filtered through a Toyo No 5C filter paper to obtain 3700mL of sandalwood hot water extract. The extract thus obtained was frozen at −40 ° C., powdered with a freeze dryer, filtered through 80mesh to obtain 50 g of hot water extract powder.

Example  2-4: Sanderuck  Preparation of Ethanol Extract

1Kg of sandalwood was washed well to remove foreign substances, and then pulverized by using a grinder. Extracted by adding 50%, 70%, and 100% ethanol, three times the weight of deodeok, was extracted for 4 hours at 100 ° C. with Toyo No 5C filter paper. After filtration, the solvent was concentrated to remove all solvents, thereby obtaining 3700 mL of extract ethanol. In addition, the extracted extract was frozen at −40 ° C., powdered with a freeze dryer, and filtered through 80 mesh to obtain 40 g of ethanol extract powder.

Example  5: Sanderuck  Preparation of Enzyme Extracts

1Kg of sandalwood was washed well to remove foreign substances, and then pulverized by using a pulverizer. After adding water three times the weight of deodeok, 0.75% of enzyme was added and the pH was adjusted to 5.0. After filtering with Toyo No 5C filter paper, 3700 mL of the extract of Sandus enzyme was obtained. The extract was frozen at −40 ° C., powdered with a lyophilizer, and filtered through 80mesh to obtain 50 g of the enzyme extract powder.

Experimental Example  One: Sanderuck  Inhibition of Cancer Cell Proliferation by Extracts Cytotoxity )

Cytotoxity measurement by MTT assay was performed by injecting 130 mL of SNU-1 (human gastric adenocarcinoma (gastric cancer cell) into 96 well plates at 1 × 10 ⁴ cells / well and then 14.5 μL / well It was added and incubated for 72hr in 37 ° C., 5% CO ₂ Incubator. Since the salt concentration contained in the sample has a great effect on the growth of cancer cells, the solution suitable for the salt concentration of each sample was prepared and used as each control. After 72hr incubation, 14.5 μL of MTT (microtetrazoline (Sigma)) solution prepared at 5 mg / mL in phosphate saline solution was added to each well, and 108.5 μL of dimethyl sulfoxide (DMSO) was added to each well, followed by ELISA (Enzyme-Linked). Absorbance was measured at 550 nm with an immunosorbent assay reader to obtain Cytotoxity (%) as the following Equation 1 as an index of cytotoxic activity.

The hydrothermal extracts, ethanol extracts, and enzyme hydrolysates prepared according to Examples 1 to 5 were shown in Fig. 1 by measuring the effect of inhibiting cancer cell proliferation according to the concentrations of 1, 3, 5, 7, 9, and 12 mg / ml. .

As shown in Fig. 1, all of the extracts of the present invention showed cancer cell proliferation inhibitory effect, among them, the hot water extract showed a cancer cell proliferation inhibitory effect and a high inhibitory activity in the overall concentration range, and the EtOH extract had a low EtOH concentration. The extract showed high cancer cell proliferation inhibitory effect. Enzyme extract according to the present invention showed the lowest cancer cell proliferation inhibitory effect in the sample sphere, it can be seen that also showed the cancer cell proliferation inhibitory effect. In the case of hot water extraction, 18.0 ~ 70.0% at 1.0 ~ 12.0 mg / mL showed the highest cancer cell proliferation inhibitory effect, followed by 16.0 ~ 52.0% at 50% EtOH extract. The increased extractor showed lower cancer cell proliferation inhibitory effect. The enzyme extract showed the lowest cancer cell proliferation inhibitory effect and showed 39% proliferation inhibition rate at 12.0 mg / mL.

Experimental Example  2: Sanderuck  Antihypertensive Effect of Extracts

Angiotensin-I Converting Enzyme (ACE) is a 400 mg sodium borate buffer (pH 8.3) in 1 g of acetone powder purified from rabbit lungs (previously obtained from rabbit lungs). After adding mL and stirring for 24hr at 5 ° C, the supernatant obtained by centrifugation was used as ACE coenzyme solution. 50 μL of each of the extract powders obtained in Examples 1 to 5 was added 50 μL of ACE coenzyme solution and 100 μL of 0.1 M Sodium borate buffer (pH 8.3), followed by preincubation at 37 ° C. for 5 minutes. 50 μL of 0.5 mM His-His-Leu was added thereto as a substrate, followed by reaction at 37 ° C. for 30 minutes, and then 250 μL of 1 N HCl was added to stop the reaction. 1.5 mL of ethyl acetate was added thereto, stirred for 15 seconds, and centrifuged at 200 × g for 5 minutes to obtain a supernatant. The supernatant was completely dried, dissolved in distilled water, and absorbed at 228 nm, and then calculated using Equation 2. The control was used as a control to prepare a solution for the salt concentration of each sample.

S: absorbance of the sample SC: absorbance of the sample control

C: Absorbance of the control control CC: Absorbance of the control control

The results of the antihypertensive effect of the above-mentioned Shandeokdeok extract is shown in FIG. The ACE inhibitory effect was similar to that of cancer cell proliferation inhibitory effect and showed the highest effect in hydrothermal extract. In addition, the EtOH extract showed a high inhibitory effect in the extract of low concentration of EtOH. The inhibitory effect was 21.6% at 1.0 mg / mL of hot water extract and 68.4% at 7.0 mg / mL. EtOH extract showed the highest efficacy at 50% EtOH and showed an inhibitory effect of 52.8% at 9.0 mg / mL. In addition, even in the case of the enzyme extract was shown to exhibit an antihypertensive effect. Furthermore, at concentrations of 7.0-12.0 mg / mL, they showed higher efficacy (40.8-52.8%) than 70 and 100% EtOH extracts.

Example  6: Sanderuck  Deodeok Functionality Effective against Anticancer and Antihypertension with Extract as an Active Ingredient Health drink  Produce

7 mL of the sandalwood hot water extract prepared according to Example 1 was mixed with 95 mL of purified water. 0.1 g of citric acid, vitamin C 0.05 g, dextrin 0.2 g, sodium citrate 0.05 g, glucose 4.0 g, white sugar 4.0 g, and 5.0 g liquid fructose were added to the deodeok mixture as a food additive with respect to the total weight of the deodeok mixture. Mix at low temperature of 5 ° C. for 1 hour. The mixed solution was filtered through a Toyo No 5C filter paper to remove foreign substances. After filtration, the mixture of Deodeok extract, purified water and food additive was preheated to 65 ° C. and then degassed at room temperature, sterilized at 95 ° C., and then filled in a bottle. The bottle filled with the mixture containing the deodeok extract was sterilized at 85 ° C. at low temperature, cooled, and then packaged to prepare a deodeok beverage.

Deodeok extract according to the present invention has been confirmed to have anti-cancer and antihypertensive activity, and thus it is easy to drink when prepared as a drink, it can be seen that effective in the prevention and treatment of anticancer and antihypertensive when taken for a long time. Particularly, when the beverage is prepared according to the present invention, all age groups may be satisfied, so that the beverage according to the present invention is easy to drink and tastes better than the prevention of anti-cancer and antihypertension with a separate drug or a refusal food. By drinking it, you will be able to prevent cancer and antihypertension. In addition, if there is a history of hypertension or cancer in the family, by taking the beverage according to the present invention for a long time, useful components will accumulate, thereby preventing cancer and hypertension, which are the main adult diseases.

Claims (21)

Deodeok extract effective in the prevention and treatment of anti-cancer and antihypertensive, characterized in that the deodeok obtained by extracting with water, alcohol, or enzyme. The anti-cancer and anti-cancer extract according to claim 1, wherein the deodeok extract extracted with water is a dehydrated hydrothermal extract obtained by extracting and filtering Deodeok by adding 2-4 times the weight of Deodeok at 90-150 ° C. for 3-6 hours. Deodeok extract effective in the prevention and treatment of high blood pressure. [Claim 2] The anti-cancer extract according to claim 1, wherein the deodeok extract extracted with alcohol is an alcohol extract obtained by extracting and filtering deodeok by adding 2-4 times the weight of deodeok at 90-150 ° C. for 3-6 hours. Deodeok extract effective for the prevention and treatment of antihypertensive. The method of claim 1, wherein the alcohol is ethanol extract effective in the prevention and treatment of anticancer and antihypertensive, characterized in that the ethanol. The extract of claim 3, wherein the alcohol is 30 to 60% of the alcohol, which is effective for the prevention and treatment of anticancer and antihypertension. According to claim 1, Deodeok extract extracted with the enzyme is added to 2 ~ 4 times the water to the weight of the deodeok and 0.5 ~ 0.9% of the enzyme is added to adjust the pH to 4 ~ 6 and then 36 ~ 38 ℃ Deodorant extract effective for the prevention and treatment of anticancer and antihypertensive, characterized in that the enzyme extract obtained by filtration with a filter paper after 4-6 hours extraction in the incubator. The deodeok extract of claim 6, wherein the enzyme is cellulase, which is effective for the prevention and treatment of anticancer and antihypertension. According to claim 1, Deodeok extract is effective for the prevention and treatment of anti-cancer and antihypertensive, characterized in that after extracting the ground using a mill. According to claim 1, Deodeok extract is effective in the prevention and treatment of anti-cancer and antihypertensive, characterized in that the powder is frozen and powdered with a freeze dryer and then filtered through a 70 ~ 90 mesh (mesh). According to claim 1, Deodeok extract is effective in the prevention and treatment of anti-cancer and antihypertensive, characterized in that the deodeok or deodeok. Deodeok extract prepared by any one of claims 1 to 10 as an active ingredient, Deodeok functional health drink effective in anti-cancer and antihypertensive, characterized in that the deodeok extract contains 0.01 ~ 0.1% by weight of the total beverage. 12. The anticancer and antihypertensive agent according to claim 11, wherein the deodeok extract is a hydrothermal extract obtained by extracting and filtering deodeok by adding 2-4 times the weight of deodeok at 90-150 ° C. for 3-6 hours. Deodeok functional drink effective for prevention and treatment. Deodeok functional health drink effective anti-cancer and antihypertensive. 12. The deodeok functional healthy drink effective in anticancer and antihypertension according to claim 11, wherein the deodeok extract is 60 Brix concentrate. The method according to claim 11, wherein the functional health drink, the deodeok further comprises 1 to 5% by weight of sugar, 0.1 to 0.5% by weight of citric acid, 0.01 to 0.005% by weight of vitamin C, 0.1 to 0.5% by weight of dextrin. Deok functional health drink effective in anti-cancer and antihypertensive. Grinding the deodeok with a grinder, Extracting the pulverized deodeok with water, alcohol or enzyme, A method for producing a deodeok functional health drink effective in anti-cancer and antihypertension, comprising the step of mixing purified water so that the deodeok extract as an active ingredient to be contained in an amount of 0.01 to 0.1% by weight based on the total beverage weight. 16. The method of claim 15, wherein the extract further comprises the step of filtering the extract, which is effective for anticancer and antihypertension. 16. The method of claim 15, wherein the step of extracting the pulverized deodeok with water comprises the step of extracting and filtering by adding 2 to 4 times the weight of the deodeok powder at 90 ~ 150 ℃ for 3-6 hours. A method for producing a deodeok functional health drink effective for anticancer and antihypertension. 16. The method of claim 15, wherein the step of extracting the pulverized deodeok with alcohol comprises extracting and filtering the deodorant pulverized product by adding 2 to 4 times the alcohol weight of deodeok for 3-6 hours at 90 ~ 150 ℃ A method for producing a deodeok functional health drink effective for anticancer and antihypertensive, characterized in that. 17. The method of claim 16, wherein the alcohol is 30-60% ethanol. 16. The method of claim 15, wherein the step of extracting the pulverized deodeok with enzyme is added to the weight of the deodeok 2 ~ 4 times the weight of the deodeok 0.5 to 0.9% of the enzyme is added to adjust the pH to 4-6 After extracting 4 to 6 hours in an incubator at 36 ~ 38 ℃ after the step of filtration with a filter paper characterized in that the anti-cancer and anti-hypertension effective method for producing a functional functional health drink effective. 21. The method of claim 20, wherein the enzyme is a cellulase, which is effective for anticancer and antihypertensive health.

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