KR20070078156A - Composition for stabilizing fructose 1,6-diphosphate trisodium salt in water phase and method for stabilizing fructose 1,6-diphosphate trisodium salt using thereof - Google Patents

Abstract

A Composition for stabilizing fructose 1,6-diphosphate trisodium salt in water phase by using a cationic polymer and an anionic polymer is provided to inhibit decomposition of fructose 1,6-diphosphate trisodium salt by outer environment such as water, temperature and light rays. The composition comprises (a) 1.0 to 20.0 wt% of fructose 1,6-diphosphate trisodium salt; (b) 0.1 to 5.0 wt% of a cationic high molecule; (c) 0.1 to 5.0 wt% of an anionic high molecule; and (d) the balance of water. The composition can further comprise 1 wt% or less, based on the total weight of the composition, of an antioxidant and any one selected from sodium hydroxide, potassium hydroxide, magnesium hydroxide and aluminum hydroxide to control pH of the composition to 6 or less. The cationic polymer is at least one selected from chitosan, lysine, arginine, cystine, polyethyleneimine and polyvinylpyrrolidone and the anionic polymer is at least one selected from gelatin, hyaronic acid, alginic acid, sodium arginate, starch, starch oxide and methyl carboxy cellulose.

Description

Composition for stabilizing fructose 1,6-diphosphate trisodium salt in water phase and method for stabilizing fructose 1,6-diphosphate trisodium salt using the same {composition for stabilizing fructose 1,6-diphosphate trisodium salt in water phase and method for stabilizing fructose 1,6-diphosphate trisodium salt using pretty}

The present invention relates to a composition for stabilizing fructose 1,6-diphosphate trisodium salt (FDP) in the water phase and to stabilize the fructose 1,6-diphosphate trisodium salt using the same It is about a method. More specifically, the present invention stabilizes fructose 1,6-diphosphate trisodium salt using cationic and anionic polymers, so that fructose 1,6-diphosphate trisodium salt is not only water, but also temperature, Decomposition by an external environment such as light rays can be effectively suppressed.

Not only does skin occupy the largest area of our body, but it is always exposed to the harmful environment of the outside. It is generally well known that skin, which serves as the body's primary protective layer, is always exposed to the risk of oxidative damage, and if it is not effectively defended, skin aging such as wrinkles is accelerated.

Fructose 1,6-Diphosphate Trisodium Salt (FDP) protects tissues from oxidative damage such as Ischemia / reperfusion or prevents ATP depletion of isolated organs. It has been used to prevent damage. Whether the FDP can protect skin cells in an environment causing oxidative damage such as UV, such as effectively protecting tissues such as the heart in a rapid environment such as surgery, furthermore, this effect directly affects skin aging. By conducting various experiments not only on skin cells but also on animals and humans to see if they can alleviate or delay, the FDP not only inhibits skin cell damage caused by UV, but also significantly suppresses or improves skin aging. Could get

However, the fructose 1,6-diphosphate trisodium salt is very unstable and has a problem of being easily decomposed by sensitively reacting to an external environment such as air, particularly oxygen, heat, and light.

Therefore, in recent years, a method of adding an antioxidant as a method for inhibiting the oxidation of fructose 1,6-diphosphate trisodium salt, to suppress the oxidation of fructose 1,6-diphosphate trisodium salt in terms of formulation A method of stabilizing in a polysulfide, a method of stabilizing in an oil-in-water type emulsion, and a method of inhibiting oxidation of fructose 1,6-diphosphate trisodium salt by using zinc sulfate and L-tyrosine together have been proposed.

However, all of the above-described methods have the following problems. That is, when using phenol derivatives such as tocopherol, which is widely used as a natural antioxidant among the methods of using antioxidants, it is known that there is some antioxidant effect, but when oxidized, the color is changed from yellow to purple generally. There are disadvantages, and the effect is relatively insignificant compared to the antioxidant power of fructose 1,6-diphosphate trisodium salt, which is not recognized as an effective antioxidant method of fructose 1,6-diphosphate trisodium salt. On the other hand, the method of using a thiosulfate analogue as an inorganic salt antioxidant is a pH of about 2 to 4 when the fructose 1,6-diphosphate trisodium salt is dissolved, in the pH range The unpleasant odor derived from the liberated sulfur dioxide gas by the equilibrium of thiosulfate and sulfur dioxide gas has the disadvantage that the value as a product is greatly reduced. There are other ways of adding chemically synthetic antioxidants, but they are not compatible with the natural concept of using fructose 1,6-diphosphate trisodium salt, and are not suitable for practical applications. There was a problem with this. In addition, in the case of stabilizing fructose 1,6-diphosphate trisodium salt in multiple emulsions, even if the fructose 1,6-diphosphate trisodium salt is stabilized in the multiple emulsion itself, most of the multiple emulsions are over time. There is a problem in that the stability is inevitably caused to decrease the potency over time. In addition, efforts have been made to stabilize the oil phase, but since it is difficult to add it to the award product, there is a problem in that the award product such as medicine or cosmetics is not easy to use.

Accordingly, the present inventors have conducted a study to solve the above-mentioned problems for stabilizing fructose 1,6-diphosphate trisodium salt, the result that the fructose 1,6-diphosphate trisodium salt and the cationic polymer In the case of encapsulating by physicochemically binding to the polymer chain using an anionic polymer, fructose 1,6-diphosphate trisodium salt is effectively prevented from being decomposed in response to external environment such as water, oxygen, heat, air, light, etc. It has been found that the present invention can be prevented, and the present invention has been completed.

Accordingly, it is an object of the present invention to provide a composition capable of effectively stabilizing fructose 1,6-diphosphate trisodium salt in an aqueous phase.

Another object of the present invention is to provide a method for stabilizing fructose 1,6-diphosphate trisodium salt using the composition described above.

In order to achieve the above object, the composition for stabilizing fructose 1,6-diphosphate trisodium salt in the water phase according to the present invention (A) fructose 1,6-diphosphate trisodium salt 1.0 ~ 20.0% by weight ; (B) 0.1-5.0 wt% of cationic molecules; (C) 0.1-5.0% by weight of anionic molecules; And (d) water as the residual amount.

Hereinafter, the present invention will be described in more detail.

Composition for stabilizing fructose 1,6-diphosphate trisodium salt in the water phase according to the present invention is (A) fructose 1,6-diphosphate trisodium salt 1.0 ~ 20.0% by weight; (B) 0.1-5.0 wt% of cationic molecules; (C) 0.1-5.0% by weight of anionic molecules; And (d) water as the remaining amount.

The composition for stabilizing fructose 1,6-diphosphate trisodium salt in the water phase according to the present invention is based on the fact that fructose 1,6-diphosphate trisodium salt is dissolved to become an anion, and fructose in the composition The 1,6-diphosphate trisodium salt and the cationic polymer form a stable acid-base complex so that fructose 1,6-diphosphate trisodium salt is first stabilized, and then the complex is secondary to the anionic polymer. It is encapsulated in to further stabilize the fructose 1,6-diphosphate trisodium salt, thereby effectively preventing the fructose 1,6-diphosphate trisodium salt from being decomposed by water, light or the like. At this time, fructose 1,6-diphosphate trisodium salt is contained in 1.0 to 20.0% by weight based on the total weight of the composition. This is because efficacy is not significantly significant at concentrations below 1.0% by weight, and at concentrations above 20.0% by weight, the use is limited due to problems such as irritation.

Since fructose 1,6-diphosphate trisodium salt may be decomposed by water in the composition before stabilization, it may be contained in a form dissolved in a polyhydric alcohol. In this case, the polyhydric alcohol used is preferably at least one selected from the group consisting of propylene glycol, glycerin, 1,3-butanediol and sorbitol, and the amount of the polyhydric alcohol is generally used in an amount of 10.0 to 30.0% by weight based on the total weight of the composition. Preferably, this is not limitative in the present invention.

In addition, although the kind of cationic polymer contained in the composition by this invention is not limited, The molecule | numerator which is harmless to a human body, having two or more amine groups in a molecule is preferable. Specific examples thereof include one or more selected from chitosan, lysine, arginine, cystine, polyethyleneimine, polyvinylpyrrolidone and the like. If too small amount is used, the complexing effect on fructose 1,6-diphosphate trisodium salt does not appear, and when the amount is high, 0.1 to 5.0% by weight of the total weight of the composition may occur due to solubility. It is preferable to contain in the amount of%.

In addition, the composition according to the present invention further contains an anionic polymer in order to completely encapsulate the fructose 1,6-diphosphate trisodium salt complexed with the cationic polymer. These anionic polymers surround the three-dimensional fructose 1,6-diphosphate trisodium salt, which forms a complex with the cationic polymer, to completely separate from water. In addition, the anionic polymer can protect the fructose 1,6-diphosphate trisodium salt because of its ability to absorb ultraviolet rays when ultraviolet rays penetrate. The anionic polymer is not limited in kind, and specifically includes one or more selected from gelatin, hyaluronic acid, alginic acid, sodium alginate, starch, oxide starch, carboxymethyl cellulose and the like. In particular, gelatin and hyaluronic acid are preferred, but gelatin or hyaluronic acid improves the strength of the capsule membrane when the molecular weight is high, while the solubility is limited and the viscosity of the composition becomes too high, which may cause problems in ease of use. It is preferable to use one having a molecular weight of about several million at, and in the case of hyaluronic acid, one having a molecular weight of about two to eight million. The amount of the anionic polymer used is preferably the same amount as the cationic polymer in order to stabilize the capsule membrane.

The composition for stabilizing fructose 1,6-diphosphate trisodium salt in the water phase according to the present invention may further contain an antioxidant, a basic compound and the like in addition to the components (a) to (d).

The composition for stabilizing fructose 1,6-diphosphate trisodium salt in the water phase according to the present invention, in order to further increase the stabilizing effect of fructose 1,6-diphosphate trisodium salt, antioxidants tyrosine, tryptophan and the like It may contain. However, antioxidants have both amine groups and carboxylic acids in their structure, and because they are acidic, they increase the acidity of the solution when the amount is high and cationic to form a complex with fructose 1,6-diphosphate trisodium salt. Since the amine group of the polymer can be blocked to reduce the stabilizing effect of fructose 1,6-diphosphate trisodium salt, it is preferably contained in an amount of 1% by weight or less based on the total weight of the composition.

In addition, the composition may further contain a basic compound such as sodium hydroxide, potassium hydroxide, calcium hydroxide, magnesium hydroxide, aluminum hydroxide in order to minimize the effect of acidity as described above. This is used within the range of adjusting the pH of the composition according to the present invention to 6 or less.

Since the composition for stabilizing fructose 1,6-diphosphate trisodium salt in the aqueous phase according to the present invention is harmless to the human body, it is formulated in the form of gel, membrane, bead, mesh or coated fiber to be used in injection, external skin preparation, etc. It can be applied in various ways. That is, when the composition stabilized with fructose 1,6-diphosphate trisodium salt with chitosan is used as an injection, the effect of activating cells and treating wounds can be expected. In addition, when the composition is coated and dried on the gauze nonwoven substrate, it may provide an effect such as a wrinkle improvement effect as a cosmetic material. In addition, the coating on the fiber is in the form of a gauze, when attached to the wound has the effect of increasing the speed of wound recovery.

Method for stabilizing fructose 1,6-diphosphate trisodium salt in the water phase according to the composition of the composition according to the present invention is as follows.

Method for stabilizing fructose 1,6-diphosphate trisodium salt in the water phase according to the present invention comprises the steps of dissolving fructose 1,6-diphosphate trisodium salt in water; Adding a cationic polymer to the aqueous solution of fructose 1,6-diphosphate trisodium salt; And adding an anionic polymer to the fructose 1,6-diphosphate trisodium salt and a cationic polymer solution.

In this case, when the fructose 1,6-diphosphate trisodium salt is simply dissolved in water, the fructose 1,6-diphosphate trisodium salt may be decomposed by water before it is stabilized, so that it is first dissolved in polyhydric alcohol. It is preferable to introduce into water after making it possible to minimize the decomposition in the preparation. In this case, the polyhydric alcohol used is preferably at least one selected from the group consisting of propylene glycol, glycerin, 1,3-butanediol and sorbitol, and the amount of the polyhydric alcohol is generally used in an amount of 10 to 30% by weight based on the total weight of the composition. It is preferred, but this is not limiting in the present invention.

 In addition, each step in the stabilization method of the present invention is preferably carried out at a temperature condition of 10 ~ 25 ℃. This is to minimize the decomposition of fructose 1,6-diphosphate trisodium salt by heat.

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples, but the present invention is not limited thereto.

[Examples 1-5 and Comparative Examples 1-2]

Furtherance Example Comparative example One 2 3 4 5 One 2 1) Glycerin - - 20 30 30 - 10 2) water 79.3 75.1 49.3 40.3 35.2 65 63.2 3) cationic molecules Lysine - - - 0.25 0.25 - 0.2 Arginine 0.1 0.1 - - - - Cystine 0.1 - - - - Chitosan 0.75 0.75 0.75 0.75 0.75 - 0.5 4) anionic molecules gelatin 0.75 0.75 0.75 0.75 0.75 - - Hyaluronic acid - - 0.1 0.25 0.25 - - 5) tryptophan - 0.1 0.5 0.4 0.4 - 0.1 6) Fructose 1,6-diphosphate trisodium salt 15 15 20 20 20 15 5 7) Sodium Hydroxide (0.5N) - 5 10 15 15 - -

<Production method>

1) First, component (2) or a mixture of (1) and (2) was placed in a beaker or flask, and component (6) was dissolved.

2) Component (3) was added and dissolved in the solution of 1) at room temperature.

3) Component (4) was dissolved in the solution of 2) above, and component (5) and component (7) were added to encapsulate the fructose 1,6-diphosphate trisodium salt of component (6).

Test Example 1 Measurement of titer of fructose 1,6-diphosphate trisodium salt

The initial titer of fructose 1,6-diphosphate trisodium salt in the composition of Examples 1-5 and Comparative Examples 1-2 was 100, and the titer of fructose 1,6-diphosphate trisodium salt after one month. Was measured at room temperature, 37 ℃ and 45 ℃ and the results are shown in Table 2. At this time, the sample was taken according to the concentration range of the standard product, put into a 100ml volumetric flask, dissolved in water, filtered through a 0.45mm filtration filter, and then injected into 25µl of IC.

Example Comparative example One 2 3 4 5 One 2 Room temperature 92 90 95 99 97 37 42 37 ℃ 89 85 90 96 90 32 35 45 ℃ 78 87 88 93 86 19 25

As can be seen from Table 2, in Examples 1 to 5 in which fructose 1,6-diphosphate trisodium salt was encapsulated with a cationic molecule and an anion molecule, it contained solely fructose 1,6-diphosphate trisodium salt. It was confirmed that the decrease in titer over time of fructose 1,6-diphosphate trisodium salt was less than that of Comparative Example 1 and Comparative Example 2 stabilized only with a cationic molecule.

As described above, the composition for stabilizing fructose 1,6-diphosphate trisodium salt in the water phase according to the present invention, the fructose 1,6-diphosphate trisodium salt to a cationic polymer and an anionic polymer It improves stability by encapsulating it, and can be effectively suppressed from being decomposed by an external environment such as water, temperature or light rays. Therefore, the composition may be usefully used in cosmetics and pharmaceuticals, and because it is an aqueous phase, it may be said to be a molecular assembly having excellent feeling.

Claims (12)

(A) 1.0-20.0 wt% of fructose 1,6-diphosphate trisodium salt; (B) 0.1 to 5.0% by weight of cationic polymer; (C) 0.1 to 5.0% by weight of anionic polymer; And (D) residual water; Composition for stabilizing fructose 1,6-diphosphate trisodium salt in an aqueous phase characterized by containing a. The composition of claim 1, wherein the composition further contains an antioxidant in an amount of 1% by weight or less based on the total weight of the composition. The composition of claim 1, wherein the composition further comprises one selected from the group consisting of sodium hydroxide, potassium hydroxide, calcium hydroxide, magnesium hydroxide and aluminum hydroxide to adjust the pH of the composition to 6 or less. The at least one polyhydric alcohol according to any one of claims 1 to 3, wherein the fructose 1,6-diphosphate trisodium salt is selected from the group consisting of propylene glycol, glycerin, 1,3-butanediol, and solitol. And dissolved in the composition. The composition according to any one of claims 1 to 3, wherein the cationic molecule is at least one selected from the group consisting of chitosan, lysine, arginine, cystine, polyethyleneimine and polyvinylpyrrolidone. The composition according to any one of claims 1 to 3, wherein the anionic molecule is at least one member selected from the group consisting of gelatin, hyaluronic acid, alginic acid, sodium alginate, starch, starch oxide and carboxymethylcellulose. . The composition of claim 1, wherein the composition is formulated in the form of a gel, membrane, bead, mesh or coated fiber. (1) dissolving fructose 1,6-diphosphate trisodium salt in water; (2) adding a cationic polymer to the aqueous solution of fructose 1,6-diphosphate trisodium salt of (1); And (3) adding an anionic polymer to the solution of (2); Method for stabilizing fructose 1,6-diphosphate trisodium salt in the water phase characterized by consisting of. 9. The stabilization of claim 8, wherein the fructose 1,6-diphosphate trisodium salt is dissolved in at least one polyhydric alcohol selected from the group consisting of propylene glycol, glycerin, 1,3-butanediol, and solitol. Way. The method of claim 8, wherein the cationic polymer is at least one selected from the group consisting of chitosan, lysine, arginine, cystine, polyethyleneimine, and polyvinylpyrrolidone. The stabilizing method of claim 8, wherein the anionic polymer is at least one selected from the group consisting of gelatin, hyaluronic acid, alginic acid, sodium alginate, starch, starch oxide, and carboxymethyl cellulose. 9. The method of claim 8, wherein each step is performed under a temperature condition of 10-25 ° C.