KR20070044760A - Rice composition having improved anti-oxidation capacity from the extract of rhus verniciflua - Google Patents

Abstract

The present invention relates to a method for producing functional rice, and to prepare a sumac extract from which allergens have been removed, and remove the substance having a molecular weight of 100,000 or more with an ultrafiltration membrane to obtain a solid having a solid content of 5-10% (w / v). The present invention relates to a rice composition prepared by the method of making a concentration of an aqueous solution, and impregnating or spraying it with pre-washed rice and drying the lacquer extract on the rice.

Sumac, Ultrafiltration, Antioxidant, Flavonoids, Fustin, Pacetin

Description

Rice composition having improved anti-oxidation capacity from the extract of Rhus verniciflua}

Sumac has a large amount of antioxidants. Among them, flavonoids such as fustin, phycetin, sulfuretin, butyne and butane can be exemplified (Korean J. Food Sci.Technol. 31 (3): 855-63 (1999), Korean J. Plant Res) 14 (3), 220-8 (2001), Food Sci. Biotechnol. 9 (3), 139-45 (2000). However, the lacquer tree contains a large amount of urushiol, which is known to have strong antioxidant activity and anticancer action, and thus has a disadvantage of inducing allergy by ingestion or skin contact (Korean J. Plant Res. 10 (3): 227-30 ( 1997)). Most studies aim to isolate and purify these specific flavonoids or to see their physiological activity by using organic solvent extracts of lacquer trees (Korean J. Food & Nutr. 14 (6), 527-). 31 (2001), Planta Med. 69: 990-4 (2003), Food Sci. Biotechnol. 9 (4): 204-8 (2000), Food and Chemical Toxicology 39: 229-37 (2001).

The Korean patent proposes a food using detox lacquer in application 10-1994-0000266, and the anti-cancer agent using urushiol is proposed in application 10-1997-0004193, and a method for removing toxicity of lacquer is disclosed in application 10-1999-0056704 , Application 10-2000-0031328, Application 10-2000-0055559, Application 10-2002-0019904, Application 10-2001-0028569, Application 10-2003-0071302. In addition, regarding the rice using the lacquer extract, the Korean patent application 10-2000-0011923 proposes a healthy rice prepared by making the lacquer extract and coating it on the rice, and the application 10-2001-0077144 also provides a simple lacquer extract. It only suggests a method of producing clean rice by impregnating with rice and drying. All of these patent applications propose a method of producing rice for health promotion by simply obtaining a lacquer extract by boiling water and then treating the lacquer without coating and impregnating it with rice or drying it.

The present invention relates to a functional rice composition using the extract of the lacquer tree and to provide a functional rice composition that can be obtained by coating the extract of the lacquer tree with various pharmacological functions to the rice to obtain the pharmacological effect of the lacquer tree when ingesting it. Aim.

Sumac ( Rhus verniciflua STOKES) is a deciduous tree belonging to the family Sumac , and is native to China, and is mainly cultivated in Southeast Asia such as Korea, Japan, and China. Specifically, lacquer is used as a natural paint because of its durability, and because of its chemical resistance, lacquer can be used as a special exterior paint used for airplane, ship light submarine optical cable. Moreover, oriental medicine has been used for a long time, and oriental medicine has been introduced in the treatment of internal tumors and women's menstrual disorders, gastrointestinal drugs, repellents, blood circulation and anti-aging folk remedies (Hota, M. 1989). World Useful Plant Dictionary, Common History (Japan) 910-2). In particular, in Korea, lacquer trees have been used for lacquer chickens, lacquer ducks, etc. (New Testament, Il-hoon Kim, Publisher: Namu, 1986). However, for those who are sensitive to lacquer, dermatitis and itching may be caused by very little contact with lacquer, and most people tend to be reluctant to make contact with lacquer. About 3 to 4 of them. Even after allergic to lacquer temporarily, the sensitivity is extremely high, and even a small amount of contact can cause symptoms of dermatitis.

The main component of the lacquer sap (Lac lacquer) is urushiol (Urushiol), its main purpose is used in lacquerware, and its appearance is a dark brown liquid, which dissolves well in organic solvents. The chemical structure is mainly composed of 15 to 17 carbon atoms in the side chain, and many unsaturated bonds exist. The main pharmacological effects of lacquer are known to be anti-cancer, antioxidant, hangover-relieving action (Food Science Journal, 31, 1997, 238-245), gastrointestinal disease, heart disease, arthritis, high blood pressure, diabetes, stroke, chronic fatigue, etc. Known. Despite these various pharmacological actions, it has been known that the commercial use is not due to allergic properties to the human body.

Until now, lacquer tree is mainly in the form of hot water obtained by heating such as chicken, rabbit, duck, etc. In this case, allergy occurs after ingestion due to urushiol, an allergen that is present in the lacquer tree. There has been and has been eating it simply because it is good for the body. Development of a method that can be ingested without side effects while maintaining the pharmacological effects of sumac has been required for the public's health recovery and health promotion, and some technologies have been developed. The present inventors and the applicant have continued the research of the sumac until now.

In the production of functional rice, Kim Young-jin suggests a method for producing rice with enhanced collagen and monascus in Korean Patent Publication No. 10-2003-0063315, and Jung Il-soo has published Korean Patent Publication Nos. 10-2003-0086518 and 10-2003-0071740 Presented a method for producing black functional rice mixed with black soybeans and sesame in fermented black rice and a method for producing fermented coffee rice containing coffee in fermented rice, and Jincheon-gun has a safflower seed extract in Korean Patent 10-0398895. It proposes a method of manufacturing functional rice using, and Lee Sook-sook proposes a method of manufacturing rice with enhanced wormwood in the Republic of Korea Patent Publication 10-2004-0052945.

Therefore, the present invention provides a functional rice that can be easily ingested in the form of stock (rice, rice) while having the pharmacological effect of lacquer tree to help maintain health.

In the present invention, the manufacture of the lacquer extract to produce a lacquer extract that does not cause allergy to remove the high molecular weight material difficult to absorb in the body by using an ultrafiltration membrane to obtain a filtrate, while spraying the washed rice in the form of liquid Due to its dryness, the extract's ingredients are coated on the surface of the rice, and when it is cooked using it, high concentrations of functional ingredients contained in the lacquer extract are contained in the rice, which can help maintain health and restore health. To provide a functional rice composition.

The sumac tree was dried and made of sawdust to increase the extraction efficiency. Then, ethanol-containing solvent was added at a ratio of 3 to 10 times the weight, and heated and extracted at 50 to 90 ° C. for 3 to 24 hours. The solvent used for the extraction is 0 to 80% (v / v) of ethanol. When the concentration of ethanol exceeds 80% (v / v), it is not easy to remove the resin components soluble in ethanol. The ethanol concentration of the extractant should be 80% (v / v) or less to facilitate the removal of the components. The amount of solvent to be added is 3 to 10 times the weight of lacquer in consideration of the extraction efficiency and yield. As the amount of the solvent added increases, the extraction yield improves, but the time and energy required for evaporation of the solvent increase significantly and the efficiency of the process decreases. The extraction temperature is 50 ~ 90 ℃, which means that the extraction time should be very long when extracted at a low temperature below 50 ℃, and heat sensitive components are easily destroyed due to high temperature. The extraction time is 3 to 24 hours, which may be longer depending on the extraction yield and temperature desired, but 24 hours is sufficient for the efficiency of the production process.

After extraction, the solids were removed by filtration. The filtrate was concentrated in vacuo to completely evaporate the solvent. Then, 1-10 times the pure alcohol was added to the weight of the solids, and dissolved at 60 ° C. for 2 hours to dissolve the soluble components. After insoluble components are filtered off. Pure alcohol is water-soluble, but it is water-soluble to add ethanol concentration of 95% (v / v) or more, but to remove insoluble components in alcohol. The main insoluble components are polysaccharides. Since the amount of alcohol added for re-dissolution should be an amount capable of dissolving soluble components, more than one multiple could dissolve most of the soluble components. The resulting solution is concentrated again to evaporate the solvent, hexane is added 1 to 3 times, mixed at room temperature and filtered to remove the components dissolved in hexane. The most soluble components in hexane can be regarded as hydrophobic components such as urushiol. In this process, most of the urushiol components known as allergens are removed. The precipitate washed with hexane is dried at room temperature to remove hexane, and then water is added 1 to 5 times the weight of the precipitate, mixed and extracted for 30 minutes to 5 hours at 40 to 80 ° C. Extract At the end of this process, the insolubles are removed and a burgundy solution is obtained. The composition of this solution is very high, 4-15% (w / w), compared to 1.5-2.5% (w / w) of conventional lacquer water extracts based on flavonoids. The solution was diluted with water so that the concentration of the solid content was 1-5% (w / v), followed by microfiltration with a filter of 0.45 microns to obtain a filtrate, which was filtered through an ultrafiltration membrane having a molecular weight cut-off of 100,000 to obtain a component having a molecular weight of 100,000 or more. Remove and get a burgundy filtrate. The filtrate obtained was reduced by 10-25% of solids compared to before ultrafiltration, and was found to have been removed by ultrafiltration as molecular weight 100,000 or more. Analysis of fustine and phycetin, known as main constituents, of the obtained filtrate using HPLC, shows that the content of fustin is 10-35% (w / w) and phycetin is 5-25% (w / w). Simple extracts are very high compared to 5-15% (w / w) of fustine and 1-5% (w / w) of picetin.

Concentrate or spray the filtrate obtained after the ultrafiltration so as to have a solid content of 5 to 10% (w / v) and coat 0.15 to 3% (w / w) with respect to the weight of dry rice on the already washed rice. It is adsorbed on the surface of the rice in a manner to obtain a rice coated with the lacquer extract of the present invention coated yellow. The coating method of the extract on the rice can be various, and among them, the method of drying after immersion and the method of spraying with fine powder and drying at the same time are mainly available. However, the scope of the present invention is not limited by the coating method. When coating lacquer extract on rice, 0.15% or more by weight is the minimum concentration to see the pharmacological effects of lacquer extract when rice is consumed as rice. If the upper limit is 3%, side effects and rice are too high. Or the color of the rice is so dark that it is negative for intake and economical. This means that in some cases adding an appropriate level of excess is not a problem.

Hereinafter, the present invention will be described in detail by way of examples. However, the scope of the present invention is not limited by the embodiment.

Example 1.

7,000 ml of 30% (v / v) spirits were added to 1,000 g of lacquer tree and extracted for 12 hours at 70 ° C., filtered to obtain a filtrate, which was dried in vacuo to completely evaporate the solvent, 75 g of extract (called 'a' extract). ) 300 ml of 95.2% (v / v) alcohol was added thereto, redissolved at 60 ° C. for 3 hours, filtered to remove insoluble components, and the solvent was evaporated to remove 43 g of precipitate (extract 'b'). Got. 100 ml of hexane was added thereto, washed, and dried at room temperature to remove residual hexane. The amount of extract obtained was 42.5 g (extract 'c'). 400 ml of water was added thereto, followed by extraction at 80 ° C. for 3 hours, followed by filtration to obtain 376 ml of a wine solution (extract 'd') having a solid concentration of 9.5% (w / v). 825 ml of water was added to make 1,200 ml of a solid concentration of 3% (w / v), filtered through a 0.45 micron filter, and filtered through an ultrafiltration membrane with a molecular weight cut-off 100,000 to remove high molecular weight material 1,300 ml ( Extract 'e'), the solids content was 2.2% (w / v), and HPLC analysis of the components showed that the content of fustin was 29% (w / w) and phycetin 9.4% (w / w). w). The solids removed by the polymer material having a molecular weight of 100,000 or more during the ultrafiltration process was 20.5% of the solids before filtration. 200 g of the rice was washed to remove water, and the filtrate obtained after the ultrafiltration was immersed in 500 ml of a liquid having a solid content of 5.5% (w / v), impregnated for 10 minutes, and the rice was dried and dried in an oven at 70 ° C. The obtained rice was yellow, and the extract was impregnated to the 0.2 mm level of the rice surface. The weight of rice was 202g and the amount of coated lacquer extract was 2.7g, 1.34% (w / w) based on the remaining filtrate.

Example 2.

The antioxidation degree of rice obtained in Example 1 was examined by DPPH method and it was found that the control group had 100-fold antioxidant activity as compared with the case where no lacquer extract was coated.

The results are shown in the following table.

sample Coated Rice Uncoated rice Radical 50% inhibitory concentration (㎍ / ㎖) 35 365

This means that the coated rice can inhibit the same level of radicals even at the concentration of 1/100 of the uncoated rice, which can be said to be 100 times more antioxidant.

Example 3.

In the case of Example 2, water was added thereto, and rice was cooked to find the antioxidant level in the same manner as in Example 2. As a result, it was found to have an antioxidant degree of 97 times that of the control group. This means that in case of rice coated with lacquer extract, antioxidant capacity remains as it is.

Example 4.

When the rice of the present invention obtained in Example 1 was fed to a nude male mouse, CanN.Cg-Fox1nu / CrljBgi, which was not immunized, and injected with a human-derived lung cancer cell line A549 to induce lung cancer, Compared with the control group, lung cancer tumor growth was 40% and tumor growth inhibition effect was 60%. .

Example 5.

As a result of feeding the coated rice of Example 1 to the rats causing dementia, the result was to find the maze at 45% shorter time than the control in the water maze test. As a result, the rice coated with the lacquer extract of the present invention was found to be used as a food for the treatment of dementia.

Example 6.

Table 2 shows the results of comparing the increase of antioxidant activity in each step by examining the antioxidant activity of the lacquer extract of Example 1 manufacturing step.

Extract a Extract b Extract c Extract d Extract e Radical 50% inhibitory concentration (㎍ / ㎖) 98 79 76 35 13

As shown in the above table, the extract of the present invention showed a very improved antioxidant effect compared to the simple extract.

The present invention is to prepare the extract of the lacquer tree with pharmacological efficacy by coating it on rice and ingesting it, thereby providing a coating rice composition to fully exhibit the pharmacological effect of the lacquer tree by ingesting rice to cancer, dementia, etc. It can be used as a meal for patients who are suffering, and can be very useful for health promotion and recovery of the people.

Claims (3)

A rice composition containing the lacquer extract with improved antioxidant activity obtained by extracting, concentrating, and filtering lacquer with water or diluted alcohol. In preparing the lacquer extract in claim 1 1) ethanol content of 0 ~ 80% (v / v) of the solvent is added 3 to 10 times compared to the lacquer powder, and the filtrate obtained by extracting for 3 to 24 hours at 50 ~ 90 ℃ to obtain a powder by vacuum drying, 2) To the ethanol concentration of more than 95% (v / v) of alcohol is added 1 ~ 10 times, extracted at 60 ℃ for 2 hours to obtain a filtrate and concentrated in vacuo to obtain a powder, 3) To this, hexane is added 1 to 3 times, mixed at room temperature, filtered, hexane is removed and dried to obtain a powder, 4) Add water 1-5 times again, extract for 30 minutes to 5 hours at 40 ~ 80 ℃ and filter to obtain a filtrate, 5) After filtering the filtrate with a 0.45 micron filter, it is filtered through an ultrafiltration membrane having a molecular weight cut-off 100,000 to remove the high molecular weight material, which is a lacquer extract obtained.        Lacquer rice composition, characterized in that the lacquer extract coated on the surface of the rice in the claim 1 is 0.15 ~ 3% (w / w) of the weight of the rice on a solid basis.