KR20070022906A - Composition and food for prevention and/or treatment of disease to liver and nerve system with extracts of Wasabia japonica - Google Patents

Abstract

The present invention relates to a composition for preventing and treating liver and neurological diseases and health foods including horseradish extract, and more particularly, to confirm the effect of the horseradish extract on the cellular protection from oxidative damage caused at the cellular level. Chronic diseases can be applied to preventive and therapeutic compositions, and horseradish extracts with these effects can be blended with foods such as chewing gum, candy, biscuits, ice cream, beverages, and chocolates to broader levels of youth, adults, and the elderly. It always relates to a composition for preventing and treating liver and neurological diseases and health foods, including horseradish extract, which can be easily consumed anytime and anywhere and can be expected to have a continuous drinking effect.

Wasabi extract, oxidative damage, cytoprotective effect

Description

Composition and food for prevention and / or treatment of disease to liver and nerve system with extracts of Wasabia japonica}

1 shows oxidative damage to hepatocytes of mice with 200 μM H ₂ O ₂ under a phase contrast microscope, and shows hepatocyte protective effect at 100-200 ㎍ / ml horseradish extract.

Figure 2 induces apoptosis due to oxidative damage to the liver cells of the mouse with 200 μM H ₂ O ₂ and showing the cytotoxic protective effect at the concentration of horseradish extract 100 ~ 200 ㎍ / ml,

3 is a pheochromocytoma-12 (PC12) cells are pheochromocytoma isolated from the adrenal gland of rats, causing oxidative damage with 200 μM H ₂ O ₂ , wasabi extract Representative effect of PC12 cell protection under phase contrast microscope at 200-300 ㎍ / ml concentration,

4 shows oxidative damage to liver cells of mice with 200 μM hypoxanthine and 0.5 mU xanthine oxidase under a phase contrast microscope, and shows hepatocyte protection at a concentration of 25-200 μg / ml of wasabi extract, where HX is 200 μM hypoxanthine and 0.5 mU xanthine oxidase caused oxidative stress in the cells,

Figure 5 shows the cytotoxic protective effect at the concentration of horseradish extract 25 ~ 200 ㎍ / ml with 200 μM hypoxanthine and 0.5 mU xanthine oxidase induces apoptosis due to oxidative damage to the liver cells of mice.

The present invention relates to a composition and food for preventing and treating liver and neurological diseases, including horseradish extract, and more specifically, wasabi extract from oxidative damage caused in liver cells of rats and PC12 cells of rats. By confirming the cytoprotective effect can be applied to the composition for preventing and treating liver and neurological diseases, by combining horseradish extract with such effects in foods such as chewing gum, candy, biscuits, ice cream, drinks and chocolate, To a wide range of adults and seniors, anytime, anywhere can be easily consumed anytime, and relates to a composition and food for preventing and treating liver and neurological diseases, including horseradish extract can expect a continuous drinking effect.

90% of diseases are caused by free radicals, and the biggest problem in the medical field since the 1990s is how to respond to free radical attacks. Free radicals are the biggest enemy of mankind to promote disease and aging. Normal oxygen stays in the body for about 100 seconds or more, while harmful oxygen (active oxygen) remains lipid peroxide, which is the main component of the cell membrane, even if only 1 million to 100 million seconds stays, which adversely affects cell and red blood cell destruction. Cells are averaging more than 10,000 harmful oxygen attacks per day. In addition, under physiological conditions, 3-10% of molecular oxygen is converted into free radicals, and most free radicals react rapidly with various molecules to affect intracellular function.

Here, active oxygen is a free radical, harmful oxygen, a molecule having a very short activity cycle, and having an unstable structure due to the loss of an electron pair. These free radicals deprive electrons from other molecules in order to stabilize themselves, and thus, the molecules deprived of their electrons also become free radicals and deprive electrons from other molecules nearby.

As the above process continues, the cells are damaged and directly connected to the disease. Free radical damage can directly and indirectly affect adult diseases such as cancer, heart disease, and brain diseases, gastric ulcers, hypertension, aging of the skin, chronic fatigue syndrome, arterial diseases, allergies, asthma, arthritis, Parkinson's disease, cataracts, diabetes, and diabetes. It is known to be a major cause of aging and diseases such as retinopathy.

The xanthine oxidase (Xanthine oxidase (EC 1.2.3.2)) used in the active oxygen generation system in the present invention is a xanthine or hypoxanthine using molecular oxygen as a hydrogen (electron) receptor. ) Catalyzes the oxidation of uric acid into uric acid, and acts as a rate-limiting enzyme in the final oxidation of all purines. Uric acid, which is a product of the xanthine or hypoxanthine and xanthine oxidase reaction, may also cause gout, and the inhibitory effect of the enzyme has biological significance as well as inhibition of free radical production. As such inhibitors of xanthine oxidase, various tannins and related phenolic substances have been reported.

Recently, studies on the development of various natural ingredients have been attempted among free radical scavengers. As a result of this research, the most noticeable ingredients are Phytonutrients, which are phytonutrients, and numerous papers are published around the world. Citrus bioflavonoids extracted from grapefruit, orange, lemon, and tangerine A component called pycnogenol extracted from pine bark and grape seeds has been reported. In addition to the above components, there are tocopherols, ascorbic acid and derivatives thereof, carotenoids, flavonoid compounds, phospholipids, browning reaction products, amino acids, peptides, proteins, spice extracts, and the like.

On the other hand, the research on the spice extract has been a field of increasing interest since the beginning of the 1940s, in particular rosemary, sage extract has been approved by the FDA. In addition to the above ingredients, many ingredients such as nutmeg, cloves, and red pepper are known as anti-drug extracts. Although there are differences in the degree depending on the type, the free radical scavenger effect is recognized, and the other, oryzanol contained in rice bran Along with Guai medicinal resin, sesame oil in sesame oil, sesamolin, sesamin, etc. are known a lot, and it is reported that such medicinal herbs and edible plants, including balsam extract, rutin, joiner.

On the other hand, wasabi (Wasabia japonica (W. koreana), Japanese horseradish) is a plant belonging to the cruciferous family of Japanese wasabi (Wasabia japonica) and horseradish. Wasabi (Horse radish, Horseradish, Cocoholearia armoracia L.).

It is a perennial herb that is wild or cultivated in the mountains and valleys of Korea's Ulleungdo and Japan. It is about 30 cm in height. The folies are heart-shaped, fermented, and the flowers bloom in May-June with white cruciferous flowers at the end of the stems or total flowers. Root is used as a herbal medicine and is called San Kyu-Geun (Wasabiae Rhizoma). The main components include rhizome, sinigrin, arylisothiocyanate, and butylisothiocyanate.

When the plant is wound or peeled off the roots, it exhibits a unique smell and spicy taste. Due to this flavor, the plant has been suitably processed and used as an appetite promoting or digestive food.

The main ingredient of the above-mentioned flavor is found to be aryl isothiocyanate, which is the main volatile component of horseradish essential oil. Such arylisothiocyanates are not present from the beginning of the plant, but are present in an inactive state of cinigrin, which is a kind of thioglucoside in the tissue, and once the tissue is cut or injured, it is a myrosulpa as an enzyme contained in the tissue. As the synigrin is hydrolyzed by the action of myrosinase, a complex enzyme of myrosulfatase and thioglucosidase, glucose, potassium hydrogen sulfate and arylisothiocyanate are produced.

In addition to promoting appetite and digestion due to taste and aroma, arylisothiocyanate has been reported for antibacterial effects. Recent research trends using spice are not only food additives but also the cosmetics industry and aromatherapy. Research is being attempted at

From the past, horseradish has been used to increase appetite, sourness, preservation, and sterilization, especially in Japan, where it is used for the poisoning of fish and algae.

On the other hand, wasabi (wasabi) kimchi manufacturing method is disclosed [Korean Patent Registration No. 10-0483249], wasabi storage bowl is known [Korean Utility Model Registration No. 20-0356802], from horseradish A stock pest control agent containing an extracted horseradish essential oil as an active ingredient is disclosed [Korean Patent Registration No. 10-0391827].

However, the technology has not yet been widely used, most of the studies using wasabi was the study on the antimicrobial effect of wasabi extract, arylisothio cyanate content of horseradish root or its components.

In particular, as the awareness of diseases caused by the oxidation of cells is increased, oxidative stress is induced by using animal cells rather than chemical reactions, and studies on cell protection efficacy by using horseradish extract are not available in Korea. There have been no published cases, and no examples have been applied to foods such as beverages, chewing gum, candy, biscuits and ice cream.

Thus, the inventors of the present invention confirmed that horseradish extract has a cytoprotective effect against oxidative stress to complete the present invention.

Therefore, in the present invention, wasabi was added to the food by adding wasabi extract to the food by escaping the conventional methods used in the limited appetite, sour health, preservation, sterilization as a spice washer, various physiologically possessed wasabi itself It not only improves the activity but also protects against the oxidative stress of cells that cause various chronic diseases, so it is especially used as a composition that has the effect of preventing and treating liver and neurological diseases, and is also added to various foods. By providing a composition for preventing and treating liver and neurological diseases, and a food which exhibits a protective action against oxidative stress of cells, including wasabi extract, which can be easily commercialized.

The present invention is characterized by a liver, neurological disease prevention and treatment composition comprising horseradish extract.

In addition, the present invention is characterized by including wasabi extract and includes a health food exhibiting a protective action against oxidative stress of cells.

Hereinafter, the present invention will be described in detail.

The present invention can be applied to the composition for the prevention and treatment of liver and neurological diseases by confirming the cytoprotective effect of horseradish extract from oxidative damage caused in liver cells of rats and PC12 cells of rats. By combining the horseradish extract with foods such as chewing gum, candy, biscuits, ice cream, beverages and chocolate, it can be easily consumed anytime and anywhere to a wide range of teenagers, adults, and the elderly. It relates to a composition and health food for preventing and treating liver and neurological diseases comprising the extract.

The present invention uses horseradish extract as a component having a protective effect against the oxidative stress of the cells that cause chronic diseases, as a composition having a prophylactic and therapeutic effect against chronic diseases, particularly liver and neurological diseases. It relates to a new use of horseradish extract to be applied to food.

In particular, in the present invention, oxidative stress with oxidative stress generated by the reaction of H ₂ O ₂ and xanthine oxidase and hypoxanthine using a hepatic cell line (Hepa 1c1c7 murine hepatoma cell line) of the mouse rather than a simple chemical reaction. ), And the results were evaluated using horseradish extract, the oxidative damage of cells due to H ₂ O ₂ began to show a cytoprotective effect at a concentration of 100 ㎍ / ml, xanthine oxidase and In case of damage caused by hypoxanthine reaction, it was confirmed that excellent cytoprotective effect appeared from 25 ㎍ / ml concentration.

In addition, the PC12 cells used in the present invention are derived from rat adrenal pheochromocytoma, biosynthesis, storage and secretion of catecholamines, and tyrosine hydroxylase (EC 1.14). .16.2)] [Greene, LA] and Rein, G. (1977) Short-term regulation of catecholamine biosynthesis in a nerve growth factor responsive clonal line of rat pheochromocytoma cells. J. Neurochem. 30: 549-555.

In particular, PC12 cells have been used as a model for the study of neuronal differentiation, chromaaffin cell function, learning-habituation, etc. [Greene, LA and Tischler, AS (1982) PC12 pheochromocytoma cultures in neurobiological research: In Advance in cellular neurobiology. vol. 3. (ed. Feroroff S.), 373. Academic Press, New York.]. In the present invention, H ₂ O ₂ induces nerve cell damage by oxidative stress on PC12 cells and confirmed the protective effect by wasabi extract.

As a horseradish extract of the present invention, both Japanese horseradish or horseradish can be used, and water, lower alcohol having 1 to 6 carbon atoms, or a mixture thereof can be used as an extraction solvent.

In the present invention, the efficacy was measured using Japanese wasabi, using a method of extracting wasabi was extracted by using ethanol as a solvent, ultrasonic irradiation. That is, the method of obtaining wasabi extract in more detail in the present invention.

First, the root of horseradish was sampled with liquid nitrogen, and then it was made into a powder by a grinder. The resultant was concentrated under reduced pressure at 45 to 55 ° C. using a rotary vacuum evaporator, and the concentrate was lyophilized to obtain a light green horseradish dried powder, which was stored at a temperature of −70 ° C.

Wasabi extract prepared by the manufacturing method as described above is excellent in the protective effect against oxidative stress induced by H ₂ O ₂ and hypoxanthine and xanthine oxidase, and chewing gum, candy, biscuits, ice cream, beverages In addition, it can be applied to chocolate, etc. By blending together in the food, a wide range of adolescents, adults and the elderly can be easily consumed anytime, anywhere, so that continuous drinking effects can be expected.

As in the present invention, when the horseradish extract is applied to food, it is preferable to use the horseradish extract essentially 0.1 to 1.0% by weight based on the total composition. If the content is less than 0.1% by weight, it is not desirable to achieve the desired effect of the present invention, while if it is added in excess of the above range, it is uneconomical and affects the taste and color of the finished product. It is not desirable because it can.

Liver, neurological disease prevention and treatment compositions comprising horseradish extract of the present invention can be administered orally or parenterally, for example, intravenous, subcutaneous, intraperitoneal or topical application during clinical administration, general medicine and health It can be used in the form of food.

Formulations for oral administration such as tablets, troches, lozenges, aqueous or oily suspensions, prepared powders or granules, emulsions, hard or soft capsules, syrups or elixirs. Binders such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin for preparation in formulations such as tablets and capsules; Excipients such as dicalcium phosphate; Disintegrants such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax. Capsules contain liquid carriers, such as fatty oils, in addition to the substances mentioned above.

In addition, liver, neurological disease prevention and treatment compositions comprising horseradish extract of the present invention can be administered parenterally, parenteral administration is by subcutaneous injection, intravenous injection, intramuscular injection or intrathoracic injection injection method . To formulate into a parenteral formulation, wasabi extract is mixed in water with a stabilizer or buffer to prepare a solution or suspension, which is formulated in unit dosage forms of ampoules or vials.

In addition, the dosage of the active ingredient is generally 1 to 50 mg / day per kg body weight of an adult patient, preferably 5 to 20 mg / day, 1 to several times at regular intervals according to the judgment of the doctor or pharmacist Preferably, it can be dividedly administered 2-3 times a day.

In addition, the present invention includes a health supplement comprising horseradish extract as an active ingredient. The health supplement is a food prepared by adding horseradish extract to food materials, such as beverages, teas, spices, gums, confectionery, or the like, encapsulated, powdered, suspensions, etc., when ingesting it brings about a specific health effect. Meaning, unlike the general medicine has the advantage that there is no side effect that can occur when the long-term use of the drug using food as a raw material.

Hereinafter, the present invention will be described in more detail with reference to Examples. The present invention is not limited by the following Examples.

Reference example: Animal cell line culture

The animal cell line used in the present invention (mouse) derived hepatocytes (Hepa 1c1c7 cell line) was purchased from the American Type Culture Collection (ATCC CRL 2026), 2 mM glutamine (glutamine), 100 U / Minimum essential medium alpha modification (α-MEM) medium containing ml penicillin G, 100 μg / ml streptomycin, 10% petal bovine serum, and the like Addition was used for the experiment while subcultured in a CO ₂ incubator controlled at 37 ° C. in a 5% CO ₂ /95% air environment.

PC12 cells are pheochromocytoma isolated from the adrenal gland of rats at 37 ° C. using RPMI 1640 with 10% horse serum, 5% fetal calf serum, 100 units / ml penicillin and 100 μg / ml streptomycin. Incubated in a CO ₂ incubator (5%).

Preparation Example: Isolation of Wasabi Extract

The test material [Japanese wasabi, Wasabia japonica ] used in the present invention was purchased from a cultivated farmhouse located in Cheorwon-gun, Gangwon-do, washed and dried, and cut into approximately 2 × 2 × 2 (width × length × thickness, cm) sizes and sieved. Powdered horseradish was pulverized immediately after the rapid freezing with crusher, and ethanol, an extraction solvent, was used as a first-class reagent, and the other reagents were US Sigma, Promega, Qiagen. ) The product and the special reagent were used.

In other words, 5 g of ethanol was added to 500 g of wasabi powder, which was repeatedly extracted three times with ultrasonic waves for 30 minutes, and then the filtrate was obtained by using a Toyo filter No. 2, and decompressed at 50 ° C. with a rotary vacuum evaporator. The concentrate was freeze-dried to obtain a light green horseradish dry powder. The sample was then used while stored at -70 ° C.

Experimental Example 1: H in mouse liver cells (Hepa 1c1c 7 cells) ₂ O ₂ Cytoprotective Effect of Horseradish Extracts Against Oxidative-induced Oxidative Stress

The same number (4.0 × 10 ⁴ cells) of mouse hepatocytes (Hepa 1c1c7 cells) were dispensed into each well of a 6 well plate, and cultured 24 hours before, followed by the preparation of the reference example. Wasabi extract was added at a concentration of 100, 200 μg / ml and simultaneously 200 μM H ₂ O ₂ was added to react for 30 minutes in a 5% CO ₂ incubator at 37 ° C. After removing all the reaction solution, the solution was washed with phosphate buffered saline, and then saline was added to form MTT (3- (4,5-dimethyl-thiazo-2-yl) -2,5-diphenyltetrazoliumbromide). After adding the concentration at 0.5 mg / ml, the cells were photographed using a phase contrast microscope, and then reacted in a 5% CO ₂ incubator at 37 ° C. for 4 hours to formformazan formed from MTT with dimethyl sulfoxide ( The cytoprotective effect of horseradish extract against oxidative damage by H ₂ O ₂ was measured by measuring the absorbance at 570 nm by dissolving in dimethylsulfoxide.

As shown in FIG. 1, when only H ₂ O ₂ was treated, mouse liver cells (Hepa 1c1c 7 cells) caused cytotoxicity due to oxidative stress and killed more than 50%.

On the other hand, the group with horseradish extract showed little change in cell morphology and cell number recovery. This can be interpreted as a protective effect against the oxidative stress of cells by wasabi extract.

In the cytotoxicity test using the MTT of FIG. 2, the growth rate of mouse liver cell line (Hepa 1c1c7 cell) was increased as the amount of wasabi extract increased as in FIG. 1.

Experimental Example 2: H in neurons (PC12), which is a pheochromocytoma derived from the adrenal gland of rats ₂ O ₂ Cytoprotective Effect of Horseradish Extracts Against Oxidative-induced Oxidative Stress

Each well of a 6 well plate is dispensed with equal numbers (4.0 × 10 ⁴ cells) of rat adrenal pheochromocytoma-derived neurons (PC12) for 24 hours. After pre-cultivation was added horseradish extract prepared in the reference example at a concentration of 200, 300 ㎍ / ml and the experiment was carried out in the same manner as in Experiment 1 and then photographed the changed cell morphology using a phase contrast microscope.

As shown in FIG. 3, PC12 cells of rats were cytotoxic due to oxidative stress, and most of the cells were killed when H ₂ O ₂ was treated. The change in cell morphology was small and the cell number recovered. This can be interpreted as a protective effect against the oxidative stress of cells by wasabi extract.

Experimental Example 3 Cytoprotective Effect of Wasabi Extract Against Oxidative Stress Induced by Hypoxanthine and Xanthine Oxidase

In the same manner as in Experiment 1, the same number (6.0 × 10 ⁴ cells) of mouse liver cells (Hepa 1c1c7 cells) were dispensed into each well of a 6-well plate, incubated 24 hours ago, and the red pepper prepared according to the reference example. Wasabi extract was added at a concentration of 25, 50, 100, 200 μg / ml and at the same time 200 μM hypoxanthine and 0.5 mU xanthine oxidase were added and reacted in a 5% CO ₂ incubator at 37 ° C. for 30 minutes. After removing all of the reaction solution, carefully wash with phosphate buffered saline solution, and then add saline solution to the final MTT concentration of 0.5 mg / ml, and photograph the changed cell morphology using a phase contrast microscope for 4 hours. peppers for a poma John reacted in a 5% CO ₂ incubator at 37 ℃ formed from MTT in the oxidative damage caused by hypoxanthine and reaction of xanthine oxidase by measuring the absorbance at 570 nm by a microplate reader and dissolved in dimethyl sulfoxide for The cytoprotective effect of the extract was measured.

As shown in FIG. 4, when only hypoxanthine and xanthine oxidase were treated, mouse hepatocytes (Hepa 1c1c 7 cells) caused cytotoxicity due to oxidative stress and killed more than 70%.

On the other hand, the group with horseradish extract showed little change in cell morphology and cell number recovery. This can be interpreted as a cytoprotective effect on the oxidative stress caused by horseradish extract, mouse hepatocytes (Hepa 1c1c7 cell) as the addition of horseradish extract increased as in Figure 4 in the cytotoxicity test using MTT of Figure 5 The growth rate of 80% or more at a concentration of 100 ㎍ / ml, 200 ㎍ / ml at both the cell type and the number, it can be seen that the same results as the untreated control.

From the above results, it was confirmed that horseradish extract has a strong cellular protective effect against oxidative stress, not only in mouse liver cells (Hepa 1c1c 7 cells) but also in PC12 cells of rats. In particular, direct oxidation by H ₂ O ₂ was observed. Wasabi extract was more effective in protecting against oxidative damage than enzyme stress.

Experimental Example 4: Toxicity Test

1) Oral Administration of Rats Acute Toxicity

Acute toxicity test was performed using 6-week-old SPF SD rats. Horseradish extract was suspended in 0.5% methylcellulose solution in two animals per group and administered orally at a dose of 1 g / kg / 1 ml. After administration of the test substance, mortality, clinical symptoms, and changes in body weight were observed. Hematological and hematological examinations were performed. Necropsy was performed to observe abdominal and thoracic organ abnormalities. As a result, there were no clinical symptoms or deaths in all animals treated with the test substance, and no toxicity change was observed in weight change, blood test, blood biochemistry test, autopsy findings, etc. As a result, all of the tested compounds did not show toxic changes up to 500 mg / kg in rats, and the oral minimum dose (LD50) was determined to be a safe substance of 500 mg / kg or more.

2) Parenteral administration toxicity test for rats

Using the same rat as the oral administration toxicity test, wasabi was administered by the muscle injection method 2 mg each. One rat was administered twice at two week intervals, and the other rat was administered twice at two week intervals, followed by one month intervals, and then again three doses at two week intervals.

Six months after the first injection, the clinical signs, body weight, body temperature, physical examination, blood cell abnormalities (haematology) and fecal abnormalities (urinalysis) were observed, but all showed normal values.

Example 1 Preparation of Chewing Gum (A)

20% by weight of gum base

Sugar 76.5 wt%

Wasabi extract (manufacturing example) 0.5 wt%

1% by weight of fruit flavor

2 wt% water

Chewing gum was prepared according to the above composition and content.

Example 2 Preparation of Chewing Gum (B)

25% by weight of gum base

Sorbitol 72 wt%

Wasabi extract (manufacturing example) 0.5 wt%

Peppermint Flavor 2.5% by weight

Chewing gum was prepared according to the above composition and content.

Example 3 Preparation of Candy (A)

54.5 wt% sugar

45 wt% starch syrup

Horseradish Extract (Preparation) 0.4 wt%

Orange flavor 0.1 wt%

Candy was prepared by the conventional method and the composition and content.

Example 4 Preparation of Candy (B)

Maltitol 53.6 wt%

45 wt% of reduced syrup

Wasabi extract (manufacturing example) 0.5 wt%

Natural Herbal Flavor 0.14% by weight

Candy was prepared by the conventional method and the composition and content.

Example 5 Preparation of Biscuits

Class 1 88 kg

Gravity Class 1 76.4 kg

16.5 kg per white

2.5 kg of salt

2.7 kg of glucose

40.5 kg of farm shortening

5.3 kg of ammo

Medium kg 0.6 kg

0.55 kg sodium bisulfite

5.0 kg of rice flour

0.003 kg of vitamin B1

0.003 kg of vitamin B2

Milk Flavor 0.16 kg

71.1 kg of water

Whole milk powder 4 kg

1 kg of substitute powdered milk

0.1 kg of calcium phosphate

1 kg of spray salt

25 kg of spray oil

Wasabi extract (manufacturing example) 1.7 kg

The biscuits were prepared by the conventional method and the composition and content.

Example 6 Preparation of Ice Cream

Milk fat 10.0 wt%

Nonfat milk solids 10.8 wt%

12.0 wt% sugar

Starch syrup 3.0 wt%

Emulsifying stabilizer (span) 0.5 wt%

Spice (Strawberry) 0.15 wt%

63.05% by weight of water

Wasabi extract (manufacturing example) 0.5 wt%

The biscuits were prepared by the conventional method and the composition and content.

Example 7 Preparation of Beverages

522 mg of honey

Chioctosanamide 5 mg

Nicotinic Acid 10 mg

Riboflavin Sodium Hydrochloride 3 mg

Pyridoxine hydrochloride 2 mg

Inositol 30 mg

Orthoic acid 50 mg

Wasabi Extract (Preparation) 1,003 mg

200 ml of water

The beverage was prepared by the conventional method and the composition and content.

Example 8 Preparation of Chocolate

Sugar 34.5 wt%

Cocoa Butter 34%

15% by weight of cocoa mass

Cocoa Powder 15% by weight

Lecithin 0.5% by weight

0.5% by weight of vanilla

Wasabi extract (manufacturing example) 0.5 wt%

The beverage was prepared by the conventional method and the composition and content.

Formulation Example

As confirmed above, horseradish extract has a cytoprotective effect against oxidative stress, so it can be formulated into a composition for preventing and treating liver and neurological diseases.

1) Preparation of Syrup

Syrup containing 0.5% (weight / volume) of wasabi extract of the present invention was prepared by the following method.

2 g of wasabi extract and 25.4 g of sugar were dissolved in 80 g of warm water. After the solution was cooled, a solution consisting of 8.0 g of glycerin, 0.8 g of saccharin, 0.04 g of flavor, 4.0 g of ethanol, 0.4 g of sorbic acid, and distilled water was prepared and mixed. Water was added to this mixture to 100 ml.

2) Preparation of Tablets

A tablet containing 15 mg of active ingredient was prepared by the following method.

250 g wasabi extract was mixed with 175.9 g lactose, 180 g potato starch and 32 g colloidal silicic acid. 10% gelatin solution was added to the mixture, which was then ground and passed through a 14 mesh sieve. It was dried and the mixture obtained by adding 160 g of potato starch, 50 g of talc and 5 g of magnesium stearate was made into a tablet.

3) Preparation of Injection Solution

Injection solution containing 10 mg of the active ingredient was prepared by the following method.

1 g of wasabi extract, 0.6 g of sodium chloride and 0.1 g of ascorbic acid were dissolved in distilled water to make 100 ml. The solution was bottled and sterilized by heating at 20 ° C. for 30 minutes.

As described above, the present invention confirmed the horseradish extract H ₂ O ₂ or cytoprotective effect against oxidative stress induced by hypoxanthine and xanthine oxidase.

According to the present invention, since the horseradish extract can inhibit the oxidative damage of the cells caused by the oxidative stress of the cells, it is applied as a composition having a prophylactic and therapeutic effect against various chronic diseases caused by the oxidative damage of the cells can do.

In addition, when the horseradish extract of the present invention is formulated with various food ingredients, such as chewing gum, candy, biscuits, ice cream, beverages, chocolate, etc. to produce a variety of functional foods anytime, anywhere, a wide range of youth, adults and seniors Ingestion can be expected to have a continuous drinking effect.

Claims (3)

Liver, neurological disease prevention and treatment composition comprising horseradish extract. Contains horseradish extract and health foods, characterized in that it exhibits a protective effect against oxidative stress of cells. The health food of claim 2, wherein the food is selected from chewing gum, candy, biscuits, ice cream, beverages, and chocolate.

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