KR20060096130A - Anti-inflammation composition including kanapanax cortex extract and ginger extract - Google Patents

Abstract

    The present invention relates to an anti-inflammatory composition comprising a thawing extract and ginger extract, the composition of the present invention includes both pharmaceutical preparations and food, cosmetic composition for skin protection. The composition of the present invention comprising the extract of the oak and ginger extract may exhibit an anti-inflammatory effect by inhibiting the biosynthesis of nitrogen monoxide and prostaglandin E2 which are important mediators in the inflammatory process.

Thawed skin, ginger, anti-inflammatory composition, prostaglandins, nitric oxide

Description

Anti-inflammation composition including Kanapanax cortex extract and ginger extract}

Figure 1 shows the anti-inflammatory effect of the anti-inflammatory composition of the thaw skin extract and ginger extract 9: 9: 1 by quantifying the amount of nitrogen monoxide produced after inducing an inflammatory response through lipopolysaccharide in RAW264.7 cell line, a macrophage line The result is.

Figure 2 is an anti-inflammatory composition of the thaw skin extract and ginger extract 9: 9 by quantifying the biosynthesis amount of prostaglandin E2, an inflammatory hormone produced after inducing an inflammatory response to lipopolysaccharide in the macrophage RAW264.7 cell line Results show anti-inflammatory efficacy.

   The present invention relates to an anti-inflammatory composition comprising a Kalopanax cortex extract and a ginger extract, and more particularly, by inhibiting the biosynthesis of prostaglandin E2, which is a central signaling agent during an inflammatory response, arthritis, gastritis, colitis, dermatitis It relates to a novel natural composition that can improve inflammatory diseases such as.

  Inflammatory diseases are bacteria-induced abscesses in which tissues invade tissues to proliferate purulent fungi, resulting in the death of white blood cells, tissues damaged by bacteria, and cell debris that accumulate in tissues. Refers to the pathological state of the place. However, in all inflammatory reactions, the immune action due to the invasion of microorganisms does not appear, and in inflammatory diseases such as degenerative arthritis and rheumatoid arthritis, the immune response occurs without infection of the microorganisms, and has the same symptoms as the inflammation.

  Until now, it is known that a lipid-derived hormone called prostaglandin plays an important role in important signal transduction pathways that develop in the inflammatory process. Prostaglandin biosynthesis is initiated by the release of arachidonic acid present in the cell membrane through the activity of phospholipase A2 activated by external stimulation. The free arachidonic acid is transformed into prostaglandin H2 by an enzyme called cyclooxygenase, which is then converted into prostaglandin E2 through several steps, and prostaglandin E2 is a hormone that stimulates immune cells again to invade the tissues of inflammatory cells. Induces an inflammatory response.

   Until now, as an important therapeutic agent for inflammatory diseases, drugs with an inhibitory mechanism of activity of phospholipase A2 and cyclooxygenase, which are important enzymes in prostaglandin biosynthesis, are mainly used. Although steroid hormones are used as inhibitors of phospholipase A2, the use of long-term or long-term use of steroidal drugs is known to cause a number of side effects. The use of drugs is extremely limited.

   Due to the excessive side effects of steroid drugs, non-steroidal anti-inflammatory drugs are often used in the treatment of general inflammatory diseases or chronic treatment. As nonsteroidal anti-inflammatory agents, acetylsalicylic acid (aspirin), ibuprofen, acetaminophen and the like are widely used. Nonsteroidal anti-inflammatory drugs are mostly composed of inhibitors of cyclooxygenase, which either completely inhibit the activity of cyclooxygenase or bind to the active site of the enzyme to produce compounds of prostaglandins and other structures. However, these nonsteroidal anti-inflammatory drugs have less side effects than steroid drugs, but are known to cause gastrointestinal disorders such as gastrointestinal bleeding during chronic intake. Since most of the chronic inflammatory diseases such as arthritis, dermatitis and gastritis take a long time to eliminate the cause and complete treatment, side effects in the use of these steroidal or nonsteroidal anti-inflammatory drugs are a major obstacle in the treatment of the disease. Works. The number of these chronic inflammatory diseases is increasing rapidly due to the aging population, social stress and increased chemical intake. Therefore, in the treatment of chronic inflammatory diseases, the development of a material that can effectively control inflammation without side effects when taking for a long time is urgently needed.

   Thawing blood refers to the bark of the oak tree (Kalopanax pictus). The oak tree is a perennial deciduous arborescent tree belonging to the Agalaceae family, and the leaves are palm-shaped, common to the Agalaceae family, and are usually divided into 5-9 branches. The oak tree grows in all areas of Korea and has strong self-sustainability and its development is very fast. The oak tree is abundant (祛風 하고), blood-blooded (活血), and pain relief because it has the effect of neuralgia low back pain, arthritis is widely used. It is known in traditional medicine to have this effect in all parts of the oak tree, but among them, the bark part, Haedongpi (海桐 皮) is most widely used as a medicinal herb. The effects of thawing blood have been reported to be mainly due to hedergaenin monodesmoside throughout the moth. Several species of substances were newly separated, and the antibacterial, antidiabetic, and anti-inflammatory effects of bark are the most widely used as medicines compared to other parts of the oak tree. Studies on the anti-inflammatory effect of such thawed blood are being conducted in part, and the Korean patent application filed by Sangji Institute (application number: 10-2002-0037750) arthritis using carlopanax saponin K, one of the active ingredients of thawed blood It provides a cure.

  Ginger (Ginger) has been used for a long time as a mating agent in herbal medicine according to the new agricultural herbal medicine and is widely used as a treatment for nausea, cough, dizziness, cold hands, low back pain, diarrhea and vomiting. In addition, ginger has been shown to have high antioxidant activity in recent studies, and also has analgesic, cholesterol-lowering and anticancer effects. In addition, ginger has a strong antimicrobial activity has long been used as a natural food preservative. Gingerol has been identified as an important active ingredient of ginger, and this component is considered to have excellent anticancer and cholesterol lowering effects and antioxidant effects.

  The present invention is to provide a composition consisting of a thawing extract and ginger extract having anti-inflammatory activity.

  Another object of the present invention relates to the provision of food, medicine and cosmetics using the composition as an active ingredient having anti-inflammatory activity.

  In order to achieve the above object, the present invention provides a composition comprising antithalamic extract and ginger extract having anti-inflammatory activity and food, pharmaceuticals and cosmetics comprising the same as an active ingredient.

  Hereinafter, the present invention will be described in detail.

  The present inventors, when mixed with 1 part by weight of hot water extract of ginger with respect to 9 parts by weight of hot water extract of thawed skin, the anti-inflammatory ability is much better than when using each extract alone, synergistic action according to the mixing ratio of the two extracts It was confirmed that the present invention was completed based on this.

  The present invention relates to a composition having an anti-inflammatory activity and to foods, pharmaceuticals and cosmetics containing the same as an active ingredient, wherein the extract may be water or a solvent extract, preferably thawed skin and ginger each using hot water of 90 ° C. or higher. It is preferable to extract for 1 hour or more, but the present invention is not limited thereto.

  The present invention also provides an anti-inflammatory composition containing the composition as well as the composition having the anti-inflammatory activity as an active ingredient. The antimicrobial and anti-inflammatory compositions can be used in food, medicine and cosmetics.

   When the composition is applied to food, it can be in the form of powder, granules, soft capsules, hard capsules, liquids, tablets, and can be blended in an amount of 0.1 to 100.0% by weight based on the total food composition.

   When the composition is applied to cosmetics, basic product cosmetics (cosmetics, creams, essences, cleansing foams, cleansing water, packs), body cosmetics (body lotions, body oils, body gels), color cosmetics (foundation, lipstick, mascara, Makeup base), hair product cosmetics (shampoo, rinse, hair conditioner, hair gel), etc., but preferably cosmetics such as lotion (skin lotion), nutrition lotion, nutrition cream, massage cream, nutrition essence, moisturizing cream, etc. The amount may be formulated in an amount of 0.0001 to 30.0 wt% based on the weight of the composition.

  When the composition is applied to a pharmaceutical formulation, it can be mixed with a pharmaceutically acceptable carrier to prepare a pharmaceutical composition exhibiting anti-inflammatory function. The pharmaceutical composition may further include conventionally used excipients, disintegrants, sweeteners, lubricants, flavoring agents, and the like, and are prepared by tablets, capsules, powders, granules, suspensions, emulsifiers, syrups, solutions, and the like by conventional methods. Or in dosage unit or multiple dosage form pharmaceutical formulations, such as parenteral formulations. Haemophilized blood and ginger extract is mixed with the composition of the present invention for the purpose of anti-inflammatory action can be formulated in 0.01% to 30.0% by weight.

  In order to confirm the anti-inflammatory efficacy of the composition containing the thawed blood and ginger extract according to the present invention, the composition for prostaglandin E2, which is an inflammation-inducing hormone that is biosynthesized after inducing an inflammatory response with lipopolysaccharide to macrophage line RAW264.7 The effect of inhibiting the synthesis and the effect of inhibiting the generation of nitrogen monoxide (NO) was investigated.

   Through the FIG. 1, the 9: 1 mixed composition of thawed blood and ginger was shown to alleviate the inflammatory response by efficiently suppressing the amount of nitrogen monoxide (NO), an inflammatory mediator, by lipopolysacchride, an inflammation-inducing substance.

   Through the Fig. 2, the 9: 1 mixed composition of thawed blood and ginger inhibited the biosynthesis of prostaglandin E2, an inflammation-inducing hormone induced by the inflammation-inducing substance lipopolysaccharide, and anti-inflammatory than when only the same dose of thawing blood or ginger extract was used. It was confirmed that it was superior to the function, and especially, it showed that the anti-inflammatory function was much higher than the 8: 2 mixed composition of Haedongpi and ginger, and the composition of 9: 1 ratio of Haedongpi and ginger was excellent in anti-inflammatory function. It was revealed that the composition is represented.

   According to the present invention, the composition consisting of thawed blood and ginger extract can improve health through anti-inflammatory action from chronic inflammatory diseases such as arthritis, gastritis, nephritis, dermatitis and colitis by inhibiting biosynthesis of inflammation-inducing hormone.

   Hereinafter, the present invention provides the following examples to aid understanding, but the following examples are merely to illustrate the present invention and the scope of the present invention is not limited to the following examples.

Example 1 Preparation of Thawing Extract

  After washing the dried thawed blood well, dried enough in a cool shade, and then added about 10 times of water to 1 part by weight and extracted for 2 hours at 121 ℃. The extract thus obtained was filtered through filter paper, concentrated under a reduced pressure concentrator, and dried to obtain a thawed skin extract.

Example 2 Preparation of Ginger Extract

  The dried ginger was washed well and then dried sufficiently in a cool shade, and then added about 10 times of water with respect to 1 part by weight, and extracted at 121 ° C. for 2 hours. The extract thus obtained was filtered through filter paper, concentrated under a reduced pressure concentrator, and dried to obtain a ginger extract.

Example 3 Preparation of Composition

 9 parts by weight of the extract obtained in Example 1 and Example 2 by weight based on the weight of the extract and 1 part by weight of the ginger extract was thoroughly mixed through a mixing process of a homogenizing mixer to complete the 9: 1 mixed composition of the thaw and ginger extract. In addition, 8 parts by weight of thawed skin extract and 2 parts by weight of ginger extract was thoroughly mixed through a mixing process of a homogenizing mixer to complete the 8: 2 mixed composition of thawed blood and ginger extract.

Example 4 Inhibitory Effect of Nitric Oxide on Inflammatory Signaling Induced by Lipopolysaccharide

  Lipo, an inflammation-inducing substance, in RAW264.7 cell row, which is a pre-cultured macrophage train, to observe the inhibitory ability of nitric oxide production of the thawed blood and ginger 9: 1 mixed composition and the thawed blood and ginger 8: 2 mixed composition obtained in Example 3 A certain amount of polysaccharide was added and the composition was added at the same time and incubated for 24 hours. Thereafter, the amount of nitrogen monoxide generated during the incubation time was compared by quantifying nitrate, an oxide of nitrogen monoxide produced from the culture solution. As shown in FIG. 1, the thaw and ginger extracts were found to have a nitric oxide production inhibitory effect in both the composition mixed at 9: 1 or 8: 2. Nitric oxide is an inflammatory mediator known to increase vascular permeability during chronic or acute inflammatory reactions to induce immune cell infiltration and promote edema. Thus, the reduction in the amount of nitrogen monoxide produced by both compositions obtained in Example 3 may alleviate the inflammatory symptoms that may occur during chronic inflammation by inhibiting the mediator of this inflammatory response.

Example 5 Inhibitory Effect of Liposomal Induced Inflammatory Hormone Prostaglandin E2 Synthesis

  The pomegranate extract obtained in Example 3 was evaluated for the inhibition of the synthesis of prostaglandin E2, an inflammation-related hormone that is increased synthetically from the macrophage line RAW264.7 cell line by lipopolysaccharide. Lipopolysaccharide was added to the cultured macrophage line RAW264.7 cell line, and at the same time, more prostaglandins were added after 24 hours after the addition of thawed skin extract obtained in Example 1, ginger extract obtained in Example 2 and two compositions obtained in Example 3. In order to inhibit the biosynthesis of the same amount of aspirin was added. Thereafter, the concentration of prostaglandin E2 from each supernatant was quantified by enzyme immunoassay to observe anti-inflammatory capacity. As illustrated in FIG. 2, it can be seen that the increased biosynthesis amount of prostaglandin E2 through lipopolysaccharide is reduced by adding thawed blood or ginger extract. In the case of the composition of 9 parts by weight of the thaw extracts presented in the present invention and 1 part by weight of the ginger extract, the biosynthesis amount of prostaglandin E2 is much higher than that of the thaw extract or the ginger extract alone. The synergistic effect between the components showed that the anti-inflammatory function was very high. In addition, in the case of the composition of 8 parts by weight of thawed skin extract and 2 parts by weight of ginger extract, the anti-inflammatory activity was also increased compared to when treated with thaw or ginger extract alone, but 9 parts by weight of the thawed skin extract presented in the present invention and ginger extract 1 It was confirmed that the activity is very inferior to the composition mixed by weight, and the appropriate ratio between the two extracts can be confirmed that it is an important factor in showing the elevated anti-inflammatory activity. In Example 4, when the thawed blood and ginger were mixed at 8: 2, it was confirmed that the amount of nitrogen monoxide, which is an inflammation mediator, was also reduced, but the decrease in the amount of the synthesis of prostaglandin E2 was not relatively high, so that sufficient anti-inflammatory function could be performed. none. However, in the case of a 9: 1 mixture of the thawed skin and ginger extract, it was determined that the antisynthetic function could be exhibited by inhibiting both the biosynthesis of nitrogen monoxide and prostaglandin E2. The mixed composition was completed.

  The present invention has anti-inflammatory activity through the inhibition of biosynthesis of inflammatory mediators nitrogen monoxide and prostaglandin E2 as a composition exhibiting anti-inflammatory function as described in the above examples. Actively utilizing the composition with an anti-inflammatory function can improve the public health through the health of the people suffering from chronic inflammatory diseases such as arthritis, gastritis, dermatitis, nephritis.

Claims (6)

  Anti-inflammatory composition comprising 9 parts by weight of haejupi extract and 1 part by weight of ginger extract as an active ingredient The anti-inflammatory composition according to claim 1, wherein the extracts of thawed bark and ginger are extracted by hot water extraction and extracted using a solvent selected from the group consisting of methanol, ethanol and butanol. The food product according to claim 1, which comprises 0.001 to 100.0% by weight of the composition. The cosmetic according to claim 1, which comprises 0.001 to 30.0% by weight of the composition. According to claim 1, Anti-inflammatory pharmaceutical composition comprising 0.001 to 30.0% by weight of the composition 2. The anti-inflammatory food, cosmetic and pharmaceutical preparation according to claim 1, wherein the composition is selected from the group consisting of arthritis, gastritis, colitis, hepatitis, dermatitis, nephritis and chronic inflammatory diseases.

Images