KR20060066179A - The cosmetic composition having the prevention of atopy - Google Patents

Abstract

The present invention relates to a cosmetic composition having a prophylactic effect of atopic dermatitis, and more particularly, to provide a cosmetic composition with excellent preventive effect against atopic dermatitis, comprising a polysaccharide extract of Korean mistletoe as a main herbal ingredient. It is.

Atopy, Korean Mistletoe, Polysaccharides

Description

{A Cosmetic Composition Having The Prevention Of Atopy}

The present invention relates to a cosmetic composition having a prophylactic effect of atopic dermatitis, and more particularly to a cosmetic composition capable of providing a prophylactic effect of atopic dermatitis, characterized in that it contains a polysaccharide extracted from Korean mistletoe.

Many skin diseases, such as atopic dermatitis, a recurrent chronic dermatitis with severe pruritus, are still known as incurable diseases that humanity must solve with cancer, AIDS, and dementia in the 21st century.

Atopic dermatitis, also known as fever, is accompanied by allergic diseases such as asthma and hay fever. Persons with atopic dermatitis have a high level of urticaria response to a large number of antigens when skin tests are performed on protein antigens such as house dust, mites, animal hair, pollen, and food, which are commonly found in the environment. Within minutes to several hours of exposure to anaphylactic shock, urticaria and angioedema, wheezing and shortness of breath, nasal congestion, runny nose, sneezing, conjunctival hyperemia and swelling, vomiting, diarrhea and abdominal pain. Occurs well in the eye and intestinal mucosa, and these unusual and inappropriate reactions are characterized by familial appearance.

The causes of atopic dermatitis are still unclear, including constitutional factors, food factors, fever maladjustment, mental problems, environmental problems, side effects of drugs, malnutrition, problems with overwork, bacterial infections, external damage, etc. The causes can be divided into various ways. In addition, genetic and immunological factors are considered to be large. Atopic dermatitis refers to a cytotoxic type classification, a reaction that is released into a type 1 allergy.

Other causes of atopic dermatitis have to be cured in different ways, with several overlapping. In particular, atopic dermatitis refers to a skin disease that is extremely toxic due to frequent use of steroid ointments and antihistamines when the fever does not diverge and accumulates on the skin, causing rashes and itching. Systemic administration of antihistamines for the control of pruritus, the first-generation antihistamines commonly used include hydroxyzine, chlorfenaramin, cyproheptadine, among which hydroxyzine has the strongest antipruritic effect. Recently, asthemazole tefenadin, cetirizine, loratadine, evastin, azelastine and the like have been used as second-generation agents that have reduced sedation as a side effect of antihistamines.

Since the cause of atopy cannot be reliably identified, all treatments are only mass therapy and it is important to maintain normal life by removing the worsening factors and reducing inflammation and pruritus.                         

The overall imbalance should be resolved in consideration of one's exact constitution, the current atopic symptoms, and the current state of the human body. The reason why uniform or uniform treatments fail to treat atopy is because it does not control the changing state of the body. Also, don't think that atopy improves just because your skin condition has calmed down temporarily.

Strengthening your fitness for atopy therapy is an essential process. In the case of a lot of weakness, nutrition should be replenished with energy as much as possible, and food should be steadily improved.

It is especially good to avoid excessive food restrictions for young children. By constitution, avoid eating harmful food for a long time, etc. However, if food limitations deteriorate the overall nutritional status of children, it's a good idea to feed anything.

In relation to the composition for conventional atopic dermatitis, cosmetic compositions containing hair vulgaris, vigor, gardenia, ephedra root extract, benzoyl peroxide, retinoic acid, betaglyciletin acid, salicylic acid, etc. are known as active ingredients, but atopic dermatitis is known. There are many problems such as not providing a satisfactory effect on dermatitis and causing a lot of irritation to the skin.

Therefore, an object of the present invention is to solve the problems of the prior art as described above, by conducting research to develop a cosmetic composition having a preventive effect of atopic dermatitis using a natural herbal medicine, the polysaccharide component extracted from Korean mistletoe It is to provide a cosmetic composition for preventing atopic dermatitis, characterized in that it contains.

The present invention for achieving the above object is characterized by containing a polysaccharide material extracted from Korean mistletoe having a cosmetic composition having a prophylactic effect of atopic dermatitis.

Hereinafter, the present invention will be described in detail.

Polysaccharides refer to polymers in which one or more aldose or ketoses are bound by glycosidic bonds. One type of monosaccharide is called a homoglycan, and two or more types are called a heteroglycan. Polysaccharides are the basic constituents of all organisms and are closely related to the various biological functions that sustain life. It is therefore abundant in algae, fungi and higher plants. The sugars present in the woody biomass, which is a higher plant, are mostly complex polysaccharides composed of hemicellulose, starch, and the like, which are heteropolymers composed of cellulose and non-cellulosic polysaccharides.

Recently, polysaccharides are importantly recognized as bioactive substances, and they have been studied in a wide range of anti-tumor, immune, anti-complementary, anti-inflammatory, hypoglycemic and antiviral activities. Because of its high dependence, it is very important to identify the structure of polysaccharides contained in plants.

The structure of polysaccharides in nature has a very simple aspect, due to the action of specific enzymes that link monosaccharides to form a polysaccharide structure. This is because the monosaccharides that make up the natural polysaccharide are mainly composed of five carbon sugars, glucose, mannose, galactose, and five carbon atoms, xylose and arabinose.

However, the necessity of studying the structure has been regarded as insignificant because of the fact that the predominant species of polysaccharides in nature is nothing more than monotonous repeats such as starch or cellulose. However, in practice, most polysaccharides are heterogeneous glycans, which are composed of two or more sugar components, have various binding patterns, and also the types and amounts of monosaccharides constituting the polysaccharides, whether the polysaccharides are linear or branched, and the linking sequence. The structure of the polysaccharide varies depending on the size of the annulus, that is, furanose or pyranose, the type of linkage, and the coordination of the anomer.

Chemical structural analysis studies have been conducted on the structural importance of polysaccharides, and the structures of glucosilanes present in the roots of mature stems and immature sugar maple leaves have been revealed. By reaction and methylation, it was found that the side branch was at O-6 and the backbone structure was a (1 → 3) bonded galactopyranosyl residue.

The study of mistletoe has been recognized in Europe and elsewhere for its activity of lowering blood pressure, vasodilation, lowering heart rate, anti-tumor, etc., and lectins present in plants that specifically bind sugars are effective in treating cancer. The research is also active. However, studies on Korean mistletoe are still incomplete.

Korean oak mistletoe is an evergreen parasitic plant planted as a tree host and has a distinct woody part. It is a plant native to oak, camellia, mineral, sesame, oak, cedar, and other species of conifers and deciduous trees. Is known to have photosynthesis and various major components and interact with the host.

The present invention is a composition for preventing atopic dermatitis, polysaccharide extract of leaves and stems of Korean mistletoe to obtain the action of inhibiting the itching of atopic dermatitis, antimicrobial action to prevent the weakened skin from exposure to bacterial infection Use in combination.

In the composition according to the present invention, polysaccharides extracted from Korean mistletoe can be divided into stems and leaves, respectively, and used in combination. That is, since the types of polysaccharides that are composed according to the tissue parts of the Korean mistletoe are different, they can be combined or each extract of polysaccharides is used.

That is, the stem and leaves are divided and pulverized, extracted with ethanol for 24 hours to remove the extract and dried. The degreasing component obtained here is ball milled for 48 hours and finely pulverized to have a particle size of about 10 to 50 µm.

After distilled water was added to distilled water repeatedly extracted for 2 hours to 3 times, filtered, and distilled under reduced pressure to leave only a small amount of water. Then, ethanol was added to induce precipitation, and the precipitate was freeze-dried after centrifugation. 15% Trichloroacetic acid (TCA) was added to the dried samples to remove proteins, precipitated with ethanol, lyophilized and added during cosmetic preparation.                     

The composition according to the present invention contains the polysaccharide extract extracted from the stem and leaves of the Korean mistletoe prepared in a volume ratio of 1: 1 to 1: 3 in a ratio of 0.1 to 5% by weight, preferably 2 to 3% by weight. It is characterized by.

The water-soluble polysaccharide extracted from the leaves of Korean mistletoe had the highest amount of galactose, and the water-soluble polysaccharide extracted from the stem was most abundantly present in the same amount of arabinose and galactose, and more specifically DEAE-cellulose anion exchange chromatography (1 → 3) Glycoside-linked galactan forms a skeletal structure, (1 → 5) Glycoside-linked alpha-L-arabinofuranosyl residues are also branched, and (1 → 6) Glycoside-bound beta-D-galactopyranosyl residues also appeared to have side branches.

The composition having a prophylactic effect of atopic dermatitis according to the present invention may have a conventional formulation, for example, to be formulated with softening longevity, astringent longevity, nourishing longevity, nutrition cream, body lotion, body oil, body essence, etc. Can be. The composition of each of these formulations may contain a variety of bases and additives necessary for the formulation of the formulation and are suitable, and the types and amounts of these components can be readily selected by those skilled in the art. Hereinafter, a skin and a lotion composition will be described as an example of application of a composition having a prophylactic effect of atopic dermatitis.

In addition, the composition having a prophylactic effect of atopic dermatitis according to the present invention can be used according to a conventional method of use, and the number of times of use may vary depending on the user's skin condition or taste.                     

Hereinafter, a composition having a prophylactic effect of atopic dermatitis of the present invention will be described in more detail with reference to examples of the present invention, but the present invention is not limited to these examples.

Example 1 Preparation of Korean Mistletoe Polysaccharide Extract

Korean mistletoe polysaccharide extract was dried and powdered using Korean mistletoe as a material.

In addition, the polysaccharide extracted from Korean mistletoe in the composition according to the present invention is divided into stems and leaves, respectively divided and used or used separately.

That is, the stem and leaves are divided and pulverized, extracted with ethanol for 24 hours to remove the extract and dried. The degreasing component obtained here is ball milled for 48 hours and finely pulverized to have a particle size of about 10 to 50 µm.

After distilled water was added to distilled water repeatedly extracted for 2 hours to 3 times, filtered, and distilled under reduced pressure to leave only a small amount of water. Then, ethanol was added to induce precipitation, and the precipitate was freeze-dried after centrifugation. The dried sample was added 15% Trichloroacetic acid (TCA) to remove the protein, precipitated with ethanol and freeze-dried.

The composition according to the present invention contains the polysaccharide extract extracted from the stem and leaves of the Korean mistletoe prepared in a volume ratio of 1: 1 to 1: 3 in a ratio of 0.1 to 5% by weight, preferably 2 to 3% by weight. It is characterized by.

Example 2: Preparation of skin lotion having a prophylactic effect of atopic dermatitis according to the present invention

A skin lotion was prepared at the composition ratio as shown in Table 1 below. The alcohol part was produced by dissolving in the order of MP, tween 20, NP-12 in alcohol distilled at 78 ° C in the order of grain, and the purified water part was glycerin, 1,3-BG, DC-193, hyaluronic acid in sterilized purified water. Prepared by dissolving in the order of, allantoin, citric acid, sodium citric acid, and then mixed with the polysaccharide extract of Korean mistletoe prepared in Example 1 to prepare a skin lotion.

Classification Raw material name Weight (% by weight) Alcohol part ethanol 7 Methylparahydroxybenzoic acid 0.1 tween-20 0.2 Nonylphenylether-12 0.2 Purified water Purified water 89.01 glycerin 0.1 1,3-butylene glycol 0.2 Alanine 193 0.1 Hyaluronic acid 0.5 Allantoin 0.05 Citric acid 0.01 Citric acid 0.03 Herbal medicine Addition of Korean Mistletoe Polysaccharide Extract 2.5 Sum 100

Example 3: Preparation of milk lotion having a prophylactic effect of atopic dermatitis according to the present invention

Milk lotion was prepared in the same manner as in Example 2.

Classification Raw material name Weight (% by weight) Alcohol part Stearic acid 2.1 Cetanol 1.3 Liquid paraffin 7.0 Squalene 0.5 Isopropyl myristate 1.0 Glyceryl Monostearate 0.5 Arasel-60 0.85 tween-20 0.1 Isostearyl-isostearate 0.5 Propylparaben 0.1 Alanine 200F / 100CS 0.1 Purified water Purified water 69.65 Triethanolamine 0.7 Propylene glycol 7.0 glycerin 3.0 Methylparahydroxybenzoic acid 0.1 Herbal medicine Korean Mistletoe Polysaccharide Extract 5.5 Sum 100

Experimental Example 1: Skin irritation test of the skin lotion according to the present invention

Apply the skin lotion prepared in Example 2 (10 m / 5 cm 2) to the forearm of 20 healthy men and women (10 males and 10 females) and shield the rest with aluminum foil for 30 minutes. Subsequently, long wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result of the observation, no side effects such as erythema occurred on the skin, and thus the skin lotion was confirmed that there is no skin irritation.

Experimental Example 2: Skin irritation test of milk lotion according to the present invention

Apply the milk lotion prepared in Example 3 (10m / 5cm2) to the forearm of 20 healthy men and women (10 males and 10 females) and shield the rest with aluminum foil. After minutes, long-wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, no side effects such as erythema occurred on the skin, and thus the milk lotion was confirmed that there is no skin irritation.

Experimental Example 3: Test for irritation confirmation of atopic dermatitis of skin lotion according to the present invention

The skin lotion prepared in Example 2 was applied twice a day to the site of atopic dermatitis on 20 adult men and women (10 males, 10 females) showing atopic dermatitis symptoms. The treatment effect was judged after 2 weeks of application. As shown in Table 4, 16 out of 20 patients showed no effect or irritation in improving the symptoms of atopic dermatitis, and no side effects from long-term administration.

Experimental Example 4: Test for irritation confirmation of atopic dermatitis of milk lotion according to the present invention

The milk lotion prepared in Example 3 was applied twice a day to the site of atopic dermatitis on 20 adult men and women (10 males, 10 females) showing atopic dermatitis symptoms. The treatment effect was judged after 2 weeks of application. As shown in Table 4, 19 out of 20 patients showed no effect or irritation in improving the symptoms of atopic dermatitis, and there were no side effects from long-term administration.

sample Very good Good No change Bad Skin lotion 2 7 7 3 Milk lotion 2 9 8 2

The cosmetic composition of the present invention through the above examples and experimental examples is to provide a cosmetic composition excellent in the preventive effect against atopic dermatitis by containing a polysaccharide as a natural active ingredient derived from the leaves and stems of Korean mistletoe.

Claims (6)

Cosmetic composition having a prophylactic effect of atopic dermatitis characterized by containing the polysaccharide extracted from Korean mistletoe as the main herbal ingredient The method of claim 1, Cosmetic composition having a prophylactic effect of atopic dermatitis, characterized in that the extracted polysaccharide of Korean mistletoe is derived from stem The method of claim 1, A cosmetic composition having a prophylactic effect of atopic dermatitis, characterized by extracting polysaccharides from Korean mistletoe The method of claim 1, Prevention of atopic dermatitis, characterized in that the polysaccharide extracted from the mistletoe of Korea is contained in a volume ratio of the extract of the stem and leaves in a ratio of 1: 1 to 1: 3, preferably 0.1 to 5% by weight, preferably 2 to 3% by weight. Cosmetic composition having an effect The method of claim 1, The polysaccharide extracted from Korean mistletoe is water soluble, and its main constituents are Galatos and Arabinos. Cosmetic composition having a prophylactic effect of atopic dermatitis, characterized in that the arabinofuranosyl residue forms a side branch, and the (1 → 6) glycoside-linked beta-D-galactopyranosyl residue also has a side branch. Dividing the stem and leaves of Korean mistletoe, respectively, and pulverizing and extracting with ethanol for 24 hours to remove the extract and drying it, Ball milling the degreasing component obtained here for 48 hours and finely pulverizing the particle size to about 10 to 50 μm, After distilled water was added to distilled water repeatedly extracted for 6 hours 2 to 3 times, filtered and distilled under reduced pressure, leaving only a small amount of water, followed by ethanol to induce precipitation and freeze-drying the precipitate after centrifugation. 15% Trichloroacetic acid (TCA) was added to the dried samples to remove proteins, precipitated with ethanol and freeze-dried. Method for producing a cosmetic composition having a preventive effect of atopic dermatitis, characterized in that it is contained in 5% by weight to 2 to 3% by weight.