KR20060045943A - Compositions comprising lycopene and phytoestrogen - Google Patents

Abstract

The present invention relates to a mixed composition containing lycopene in order to alleviate or eliminate the side effects of phytoestrogens, especially isoflavones and pomegranate extracts, which are taken for various purposes. Due to the side effects, it is possible to prevent and treat acne, seborrheic dermatitis, blemishes, menopausal disorders that are difficult to treat, and have an excellent effect on removing marks of acne or seborrheic dermatitis.

In addition, at least one substance selected from the group consisting of vitamin B, vitamin C, a complex of vitamin B and C, vitamin E, squalene, beta glucan, fermented soybean extract, ginkgo biloba extract, beta carotene, ogalpi, and lizveratrol Not only can be used as a skin care agent to clean and smooth the skin as it can enhance the effect, but also lycopene to alleviate or eliminate the side effects of all phytoestrogens, especially isoflavones or pomegranate extracts. It can be used as a mixed composition that can be taken together.

Phytoestrogens, isoflavones, pomegranate extracts, lycopene, acne, seborrheic dermatitis, menopausal disorders, premenstrual syndrome

Description

Compositions Comprising Lycopene and Phytoestrogens {Compositions Comprising Lycopene and Phytoestrogen}

Figure 1 is a photograph showing the case of erythema caused by side effects of isoflavones in menopausal women.

Figure 2 is a photograph showing the erythema and cheek rashes on the back of the nose appeared as a side effect of acne patients.

Figure 3 is a picture of the side effects of Figure 2 disappeared by taking together isoflavones and lycopene according to the present invention.

Figure 4 is a photograph showing the side effects of taking isoflavones and lycopene according to the present invention.

Figure 5 is a photo of the side effect of Figure 4 removed by administering with vitamin C.

Figure 6 shows the skin brightness after taking isoflavones according to the present invention.

Figure 7 shows the skin brightness after taking isoflavones and lycopene according to the present invention.

Figures 8a to 8d is a picture of the skin treated with acne marks after taking isoflavones and lycopene according to the present invention, Figures 8a and 8c before treatment, Figures 8b and 8d are photographs after treatment.

The present invention relates to a mixed composition comprising lycopene and phytoestrogens, and more particularly, to alleviate or eliminate side effects that may occur when a long-term or large dose of phytoestrogens, especially isoflavones and pomegranate extracts, taken for various purposes. It relates to a mixed composition comprising lycopene and phytoestrogens, in particular isoflavones or pomegranate extracts, in combination with lycopene.

Phytoestrogen is found in legumes, peanuts, pine nuts, green vegetables, and rice. It is also called phytoestrogen because it has a similar function and form to female hormone estrogen. Among them, it is possible to prevent and treat various menopausal disorders which may appear after isoflavone menopause. That is, by changing the menstrual cycle can prevent and treat premenstrual syndrome, can prevent or treat cancer, and is known to be good for preventing brain aging of postmenopausal women.

In addition, cerebral neurotransmitters such as endorphins and serotonin increase and calcium absorption increases, which is effective for cardiovascular diseases such as osteoporosis, obesity, anti-aging, cell membrane and vascular wall strengthening, heart disease, hypertension, arteriosclerosis, seborrheic dermatitis, blemishes It has a preventive and therapeutic effect, which can make your skin clean.

Moreover, isoflavones have a function of inhibiting the activity of 5-alpha-reductase, and thus many patents have been applied for cosmetics for the treatment of acne (WO 00/22728, WO 99/48496, WO 01/52840, WO 98 / 08503).

Such isoflavones are typically contained in soybean, and the representative ingredients thereof are daidzein, genistein, genistin, and glycite.

However, in order to achieve the effects described above, isoflavones should be taken in a certain amount or more, and the longer the dose, the skin becomes very turbid, itchy, rough, rashes occur, sometimes the gums swell, and erythema This makes it difficult to take long-term, especially acne is difficult to cure, as well as a lot of side effects such as shoulder pain (肩痛) occurs.

The side effects of taking isoflavones go away when you stop taking them. However, in the acne treatment that is noticeable and easy to see, when you put on isoflavones and various side effects appear to stop the acne treatment, the acne disappears again. Therefore, isoflavone alone is not easy to treat acne.

As a result of experimenting with isoflavones, the inventors found that the cosmetic type had no or very little effect when sweating, and when used to see the effects on acne, itching, facial millet rash, erythema, and facial inflammation In addition, facial anesthesia was observed when high concentrations were used. It is very difficult to continue using these side effects, making acne difficult.

Even when taken orally, the skin becomes very turbid, rough, itchy, erythemaous, inflamed, and large rashes occur when applied with cosmetics, and in severe cases, pain and swelling of the gums. . As a side effect, it is also difficult to continue taking isoflavones, which makes it difficult to treat acne.

In addition, even when used for the purpose of treating menopausal disorders, osteoporosis, the face becomes very turbid, erythema occurs, severe rashes occur and often refuse to take.

Isoflavones, due to these serious side effects, it is difficult to produce a product that is particularly effective for acne.

Another phytoestrogens are pomegranate extracts, which are mild in order to achieve their intended purpose, but tend to become cloudy and erythema like isoflavones.

Therefore, the present inventors can prevent and treat acne, seborrheic dermatitis and blemishes that are very difficult to treat due to side effects of phytoestrogens, especially isoflavones and pomegranate extracts, when taken together with lycopene. The present invention has been completed by confirming that the treatment can make the skin clean and smooth.

The main object of the present invention is lycopene and phyto which is effective in eliminating or alleviating various side effects such as acne, seborrheic dermatitis, and blemishes when taking phytoestrogens, especially isoflavones or pomegranate extracts alone, which are used for various purposes. It is to provide a mixed composition containing estrogen.

In addition, another object of the present invention is to provide a mixed composition containing lycopene and phytoestrogens effective to remove or alleviate the stimulation of acne marks, seborrheic dermatitis.

In order to achieve the above object, the present invention provides a mixed composition comprising lycopene and phytoestrogens effective to remove or alleviate various side effects such as menopausal disorders, osteoporosis, acne, premenstrual syndrome, seborrheic dermatitis.

The present invention also provides a mixed composition comprising lycopene and phytoestrogens effective to remove or alleviate the stimulation of acne marks, seborrheic dermatitis.

Although the exact pharmacological mechanism of the composition of the present invention has not been demonstrated, when owners of skin diseases such as acne consume phytoestrogens, especially isoflavones or pomegranate extracts for treatment, the extracts do not circulate well in the blood vessels. The treatment effects such as acne are reduced, and these clusters appear as side effects such as inflammation, especially in the face closest to the epidermis. In order to eliminate the above side effects by taking lycopene with excellent blood circulation to help blood circulation of isoflavones or pomegranate extract to maximize the therapeutic effect. As such, when lycopene and isoflavone or pomegranate extract are taken as a mixture, it can be seen that the treatment of symptoms such as acne or seborrheic dermatitis, which is easily recognized as a face, ultimately smoothes the skin. If any of the isoflavones or pomegranate extracts are taken in the morning for any purpose, itching and rashes occur, especially in the evening, and the lycopene taken together does not seem to cause the above side effects. At this time, the side effects are getting worse.

The addition of lycopene to these phytoestrogens, especially isoflavones and pomegranate extracts, also adds vitamin C, a complex of vitamins B and C, vitamin E, and squalene to aid blood circulation.

In addition to this, it is more effective to add ogalpi, resveratrol, ginkgo leaf extract or fermented soybean extract, which are known to help blood circulation.

In addition, acne patients usually have a weak immune function, so the composition that adds beta-glucan (such as Ganoderma lucidum extract) to enhance the immune function is preferred, beta carotene is also effective to convert to vitamin A.

Hereinafter, the components of the mixed composition according to the present invention will be described in detail.

In addition to isoflavones, phytoestrogens include naringin, naringenin, hollyhock, and mallow, which are contained in oranges, citron and lemon grapefruit. Shamrock's biochanin A (biochanin A), etc., all of these are effective against acne, lycopene may be taken together. In addition, phytoestrogens contained in pomegranate are estrogens with the same structural characteristics as humans.

The phytoestrogens used in the present invention can be used as foods, and in particular, isoflavones of soybeans, and isoflavones include daidzein, genistein, genistin, and glycidin. Each or a mixture thereof may be used, or commercially available isoflavone-containing soybean powder may be used.

Genistein is also known as Genistein aglucone, and Genistein is also called Genistein glycosides. Genistin is converted to genistein by microorganisms in the intestine. Dizyne includes dizygen aglucon and dizygen glycoside.

Lycopene is found in tomatoes, watermelons, and other vitamins, along with vitamin C, vitamin E, and carotene, and is a powerful antioxidant that prevents cardiovascular disease, exerts anti-cancer effects, and is known to be good for the prostate and for skin care. Tomato extract containing 5% of lycopene is commercially available.

Pomegranate extract, which is mainly used for treating menopausal disorders, is commercially available containing 50% of the extract stock solution.

The composition according to the present invention may be used by adding other ingredients or excipients in addition to the above.

Ginkgo biloba extract refers to Ginkgo biloba extracts, and ginkgo biloba extract, which is formulated and standardized to have a total ginkgo flavone glycoside of 24%, is commercially available.

The composition of the present invention for oral administration preferably further comprises beta glucan (β-glucan), which is preferably obtained from mushrooms or microorganisms, and among them, ganoderma lucidum and the like are particularly preferred. The pharmacological effects of ganoderma lucidum, such as immune enhancement and anticancer activity, are well known (Sone, Y., Okuda, R., Wada, N., Kishida, E., Misaki, A. 1985., Agric. Biol. Chem , 49: 2641-2653).

In addition, as the beta glucan of microorganisms, cell walls of yeast, cell walls of lactic acid bacteria, polysaccharides produced by microorganisms, and the like can be preferably used. In particular, the polysaccharide produced by the genus Oreobacidium is already used in food as its main ingredient is beta glucan.

In addition, the composition of the present invention for oral administration preferably further comprises a soybean fermented extract. Soybean fermented extract is a protease produced by Bacillus , and it is widely used in dietary supplements because of its ability to dissolve blood clots (Sumi, H., Maruyama, M., Yoneta, T., Mihara, H. 1983, Experientia 43: 1110-1111).

In addition to the above components, the composition of the present invention for oral administration preferably further comprises regalvera (l veravera) with blood circulation function. These are all widely known as antioxidants. In addition, the organolpi, in particular, gogagalpi is shown in the patent No. 0284120 obtained by the present inventors excellent efficacy in skin beauty, may be used in combination with phytoestrogens.

In the case of oral administration of the composition according to the present invention, representative isoflavones are preferably 5 to 200 mg of soy isoflavones (30% isoflavones), preferably 10 mg to 160 mg, based on an adult daily dose. That is, about 1.5 to 67 mg of pure isoflavones (100%) is the appropriate dosage.

However, when the average body weight is 60 kg or more, the dose is increased in proportion to the body weight, and the same applies to the other components below.

Pomegranate extract (pomegranate extract content 50%) is a proper dosage of 20 mg to 4 g, preferably 20 mg to 3 g. DiZane, Genistein, Genistin, Glycsteine, Naringin, Naringenin, Proretin, Quercetin and Biochanin are preferably from 1 to 80 mg per adult daily dose.

Lycopene (content 5%) is preferably 5 to 200 mg, preferably 5 to 160 mg.

Vitamin C or a complex of vitamin C and B is 100 mg to 1 g and vitamin E is 100 to 1000 IU, which is added to the composition for oral administration of the present invention to further eliminate side effects based on the daily dose of an adult. And squalene is preferably from 0.1 to 2 g.

In addition, beta glucan is 3-100 mg of Ganoderma lucidum fruiting body powder, 5-100 mg of microorganism producing polysaccharide (betaglucan), soybean fermentation extract powder (enzyme activity 13000 FU / g) 3 to 200 mg, ginkgo leaf extract is 3 To 50 mg (1.7 to 25 mg in total ginkgo flavone glycosides), and organolpi is more preferably added at 5 to 100 mg as prickly torches.

In addition, it is preferable to add 1 to 100 mg of lizveratrol, and 2 to 500 mg of beta carotene is preferable.

The above conditions are in the range of the maximum amount, and in case of using less than the minimum value or using the excess amount, the synergistic effect with other components is lowered or the effect is lowered.

Hereinafter, the present invention will be described in detail.

The following compositional examples and experimental examples are merely illustrative of the present invention and do not limit the scope of the present invention.

Compositional examples of the composition for oral administration according to the present invention are as follows.

<Configuration example 1>

Soy isoflavones (with 30% isoflavones) ------------------ 25 g

Lycopene (containing 5% lycopene) ----------------------------------- 60 g

The composition was compressed into 2000 capsules.

<Composition Example 2>

Soy Isoflavones (30%) ----------------------------------- 25 g

Lycopene (5%) --------------------------------------------- 60 g

Vitamin B and C Complex ------------------------------------- 189 g

The composition was compressed into 2000 capsules.

<Composition Example 3>

Soy Isoflavones (30%) ----------------------------------- 25 g

Lycopene (5%) --------------------------------------------- 60 g

Vitamin C ------------------------------------------------ 250 g

Ganoderma lucidum extract ----------------------------------------- 10 g

The composition was compressed into 2000 capsules.

<Composition Example 4>

Soy Isoflavones (30%) ----------------------------------- 250 g

Lycopene (5%) --------------------------------------------- 60 g

Squalene ----------------------------------------------- 150 mg

Ganoderma lucidum extract ----------------------------------------- 10 g

Soybean Fermented Extract ------------------------------------------- 10 g

Ginkgo Biloba Extract ------------------------------------------- 5 g

Prickly Pear Extract ---------------------------------------- 5 g

Lisveratrol -------------------------------------------- 10 g

Beta-carotene ---------------------------------------------- 10 g

The composition was compressed into 2000 capsules.

<Composition Example 5>

Soy isoflavones (30%) ------------------------------------ 25 g

Lycopene (5%) --------------------------------------------- -60 g

Vitamin E ------------------------------------------------ 100 IU

Pomegranate Extract ---------------------------------------------- 100 g

The composition was compressed into 2000 capsules.

You may substitute 100 g of pomegranate extract instead of 30 g of isoflavones from the composition examples 1 to 4. However, tablets should be 3000 or 4000 for ease of taking.

 <Composition Example 6>

Narinjin ------------------------------------------------- -5 g

Lycopene (5%) --------------------------------------------- 60 g

Vitamin B and C Complex ------------------------------------- 189 g

Ganoderma lucidum extract ----------------------------------------- 10 g

Beta-carotene (5%) ---------------------------------------- 10 g

Lisveratrol -------------------------------------------- 10 g

The composition was compressed into 2000 capsules. In the above-mentioned complex of vitamins B and C, the ratio of products on the market in general is good. For example, the content of 754 mg is 15.5 mg of thiamine nitrate, 10 mg of riboflavin, 5.2 mg of pyridoxine hydrochloride, 5 µg of cyanocobalamin, 600 mg of ascorbic acid, 23.2 mg of calcium pantothenate, and 100 mg of nicotinic acid amide.

The present inventors conducted the following experiment to prove the effect of the composition according to the present invention.

Experimental Example 1.Check the occurrence of side effects when taking isoflavones and lycopene

10 people each divided into Group 1 and Group 2, taking 60 mg of isoflavones (containing 30% total isoflavones), 60 mg of isoflavones + 40 mg of lycopene (containing 5% total lycopene) (group 2) Daytime skin condition was observed, and the results are shown in Table 1 below.

Severe side effects were set to 10, and no cases were set to 0. Face itch, erythema (see FIG. 1), rashes, skin roughness, and shoulder pain were seen as side effects, but face turbidity was also referred to.

TABLE 1

group Skin condition Degree of Side Effects Group 1 Worse 8 Group 2 Much better 2

Almost all of the group 1 taking isoflavones became cloudy, rough, itchy, and six had erythema or rashes and two shoulders. However, in group 2 with isoflavones and lycopene, the skin became whiter and smoother, and almost all of them were satisfied. I could hardly feel my shoulder, but there were two cases of facial erythema.

These shoulders and facial erythema were eliminated when Group 2 took 500 mg of vitamin C, or 378 mg of vitamin B or C complex (composition of composition 2), vitamin E 200 IU, or 0.3 g of squalene. . Premenstrual syndrome also showed a similar trend.

1 is a photograph of the erythema next to the eye of the facial erythema as a side effect of Group 1.

Experimental Example 2. Acne Treatment Check

Patients with severe acne and seborrheic dermatitis were examined in 10 patients in Group 1 (acne) and 2 (acne), and 2 patients in the rest. Soy isoflavones (containing 30% total isoflavones) 60 mg (group 1) per day, isoflavones 60 mg + lycopene (containing 5% lycopene) 40 mg (group 2), isoflavones 60 mg + lycopene 40 mg + ganoderma lucidum Experiments were conducted for 4 weeks, taking 20 mg of extract (betaglucan) (group 3), 60 mg of isoflavone + 40 mg of lycopene + 20 mg of betaglucan polysaccharide (Group 4) in oreovaciddium . Before the experiment, the severe condition was set to 10 and completely treated, and the case was 0, and the severe side effects were set to 10, and the case was 0, and the experimental results are shown in Table 2 below.

TABLE 2

group  Treatment effect  Degree of Side Effects Week 0 2 weeks 4 Weeks Week 0 2 weeks 4 Weeks Group 1 (acne) 10 7 6 0 3 8 Group 1 (dermatitis) 10 8 6 0 4 8 Group 2 (acne) 10 5 3 0 One 3 Group 2 (dermatitis) 10 4 2 0 One 3 Group 3 (acne) 10 4 2 0 0 2 Group 3 (dermatitis) 10 4 2 0 0 2 Group 4 (acne) 10 4 2 0 0 2 Group 4 (dermatitis) 10 4 2 0 0 2

As shown in Table 2, in Group 1, although there is a therapeutic effect, side effects were large. Almost everyone became cloudy with isoflavones, and facial erythema and rashes appeared in eight patients. There were two people who said itchy face.

FIG. 2 shows that acne subsided to the face of acne patients in Group 1, but a rash appeared on the nose and cheeks. However, Group 2, with lycopene added, became whiter and smoother, and almost all were satisfied with it. Acne treatment effect was more than twice that of Group 1. This is because lycopene does not show any rashes, so there are some areas that are not counted as acne. Unlike menopausal men, no one complained of pain.

The beta-glucans resulted in better acne treatments with fewer side effects.

3 is a case where the isoflavones and lycopene of Group 2 of Experimental Example 2 were taken together for 4 weeks after the experiment of FIG. 2, the face was very clean.

As described above, it was found that the excellent blood circulation of lycopene prevents or reduces side effects caused by partial isoflavone aggregation.

In other words, as can be seen in Table 2, including the lycopene beta glucan is the main component of Ganoderma lucidum, Oreobashidium culture polysaccharides seem to increase the immune function to increase the immune function to increase the effect even better.

Subsequent experiments showed that when only isoflavones were used alone, many side effects such as rough skin, turbid skin, itching, rashes, shoulder pain, and gum pain appeared. I noticed it disappeared or nearly alleviated. It was also more effective to add beta glucan-containing substances that enhance immune function.

Therefore, these mixtures were much more effective. In most cases, menopausal disorders and acne have to be taken for more than 1 month to treat isoflavones.

Increasing the dose of isoflavones increases the side effects to increase the effectiveness of treatment, and taking lycopene together almost eliminates these side effects.

Of course, very light acne, such as isoflavones can be cured without major side effects, but if you are worried about acne, isoflavones are easy to treat without side effects. Not only is the treatment less effective, but as a side effect, acne disappears as often as rashes.

Experimental Example 3. Determination of isoflavone dosage for acne treatment

The appropriate dose level for acne patients was investigated. A daily dose of 5, 10, 20, 40, 60, 100, 120, 160, 200 mg of isoflavones (containing 30% of total isoflavones) should be taken for 4 weeks in the morning and evening at two concentrations. It was. One month later, one of the previous experiments and one new acne test were added to take each isoflavone concentration and lycopene 20 mg each morning and evening. Each group was expressed by isoflavone concentration, and when lycopene was added, 'L' was added to each isoflavone concentration. Before the experiment, the severe condition was set to 10 and completely treated as 0. The severe side effect was also set to 10 and no case was 0. The experimental results are shown in Table 3 below.

TABLE 3

Group (mg)  Treatment effect  Degree of Side Effects Week 0 2 weeks 4 Weeks Week 0 2 weeks 4 Weeks 5 0 10 10 0 0 One 5+ Lee 0 10 9 0 0 0 10 0 10 9 0 One One 10+ Lee 0 9 8 0 0 One 20 0 9 8 0 2 4 20+ Lee 0 8 6 0 One One 40 0 8 7 0 3 7 40+ Lee 0 6 4 0 One 2 60 0 7 6 0 3 8 60+ Lee 0 5 3 0 One 3 100 0 6 5 0 5 9 100+ Lee 0 4 2 0 3 5 120 0 5 4 0 6 9 120+ Lee 0 3 2 0 3 5 160 0 5 4 0 7 9 160+ Lee 0 3 2 0 4 6 200 0 5 0 8 200+ Lee 0 3 0 5

As shown in Table 3, the higher the concentration of isoflavones alone, the better the effect but the greater the side effects. However, when lycopene is administered with 40 mg, the side effects can be alleviated. In Table 3, the isoflavone dosing concentration is effective in the range of 5 to 200 mg based on the adult daily dose, preferably 10 to 160 mg.

Experimental Example 4. Determination of Lycopene Dose for Acne Treatment

The appropriate dose level for acne patients was investigated. Lycopene (5% total lycopene content) is daily dose of 5, 10, 20, 40, 60, 80, 120, 160, 200 mg divided into two groups at morning and evening for 4 weeks 60 mg isoflavones Take with you. The severe condition before the experiment was 10, the complete treatment was 0, and the severe side was 10, and the absence was 0. The experimental results are shown in Table 4 below. Each group representation is represented by lycopene concentration.

TABLE 4

Group (mg)  Treatment effect  Degree of Side Effects Week 0 2 weeks 4 Weeks Week 0 2 weeks 4 Weeks 5 0 7 6 0 2 6 10 0 7 5 0 2 5 20 0 6 4 0 2 4 40 0 5 3 0 One 3 60 0 5 3 0 0 One 80 0 5 3 0 One One 120 0 5 3 0 One One 160 0 5 2 0 One One 200 0 5 2 0 One 2

As shown in Table 4, the higher the concentration of lycopene, the better the acne treatment effect and the side effects disappeared, but the administration of lycopene 80 mg or more occasional spots such as freckles on the face. These spots sometimes occurred slightly at 80 mg, and the frequency was slightly higher at higher concentrations of lycopene. That is, the dosage of lycopene is 5 to 200 mg, preferably 5 to 160 mg, based on the daily dose of the adult.

Experimental Example 5. Confirmation of the side effect removal effect (1)

In Experimental Example 2, patients with severe abnormal acne and seborrheic dermatitis had side effects when taking isoflavone (30%) 60 mg + lycopene (5%) 40 mg twice a day. Unlike group 1 in Experimental Example 2, most of the side effects were rarely seen, but mainly red erythema appeared on the ridge of the nose (however, it does not appear well in the picture compared to Figure 2), and is said to be slightly sick over time Mild symptoms of side effects. Also rashes occasionally occurred (see FIG. 4).

In order to eliminate these side effects, the experiment was carried out as follows.

Based on isoflavone 60 mg + lycopene 40 mg, add 500 mg of vitamin C (group 1), 400 IU of vitamin E (group 2), 0.3 g of squalene (group 3) 4 weeks. Before the experiment, the severe condition was set to 10 and completely treated as 0. The severe side effect was also set to 10 and no case was 0. The experimental results are shown in Table 5 below.

TABLE 5

group  Treatment effect  Degree of Side Effects Week 0 2 weeks 4 Weeks Week 0 2 weeks 4 Weeks Group 1 (acne) 10 4 2 0 0 0 Group 1 (dermatitis) 10 3 One 0 0 0 Group 2 (acne) 10 4 2 0 0 0 Group 2 (dermatitis) 10 3 One 0 0 0 Group 3 (acne) 10 4 2 0 0 0 Group 3 (dermatitis) 10 3 One 0 0 0

As shown in Table 5, there was no side effect (erythema), such as runny nose, while there was a therapeutic effect in each group. In addition, in the case of B and C complex (composition of the composition 2) instead of the vitamin C of group 1, no adverse effects appeared. At this time, the content of vitamin B and C was as follows. The daily dose was 8 mg thiamine nitrate, 5 mg riboflavin, 2.6 mg pyridoxine hydrochloride, 3 μg cyanocobalamin, 300 mg ascorbic acid, 12 mg calcium pantothenate, 50 mg nicotinamide. In Experimental Example 3, it was thought that the addition of vitamins and squalene resulted in better blood circulation so that the isoflavones did not stick to specific areas of the face.

Figure 5 is a picture disappeared by taking the vitamin C 500 mg when the rash appeared when taking only 4 weeks isoflavones and lycopene. As shown in FIG. 5, the effective doses of these experiments were 100 mg to 1 g of vitamin C, 100 to 1000 IU of vitamin E, and 0.1 to 2 g of squalene based on the daily dose of the adult. And it was better to add these vitamins and antioxidants to eliminate the side effects.

Experimental Example 6. Confirmation of side effect elimination effect (2)

Referring to Experiment 5, the acne patients were treated with 60 mg of isoflavone (30% isoflavone) + 40 mg of lycopene (5% lycopene) + 377 mg of a complex of vitamin B and C, and ganoderma lucidum as a beta glucan. Extract 20 mg (group 1), soybean fermentation extract with thrombolytic function 20 mg (group 2), ginkgo biloba extract 20 mg (group 3), prickly pepper 20 mg (group 4), lizberarol with blood circulation function 10 mg (group 5), beta-carotene 20 mg (group 6), and pomegranate extract 200 mg (group 7) were added to each group of 2 people, divided twice a day for 4 weeks. Their acne treatment and skin care effects are shown in Table 6 below.

TABLE 6

group  Treatment effect  Degree of Side Effects Week 0 2 weeks 4 Weeks Week 0 2 weeks 4 Weeks One 0 3 0 0 0 0 2 0 3 0 0 0 0 3 0 3 0 0 0 0 4 0 3 0 0 0 0 5 0 3 0 0 0 0 6 0 3 0 0 0 0 7 0 2 0 0 0 One

Looking at Table 6, it was shown that the acne treatment effect is excellent in all groups, yet there are no side effects. But in terms of skin beauty, skin condition improved in order of 1> 2> 3> 4> 5> 6> 7. Therefore, beta glucan, soybean fermented extract, ginkgo biloba extract, ogalpi (gaciogalpi), lisveratrol, beta-carotene, pomegranate extract, etc. were better added, and mixed addition was also good in the future experiments. These additions were based on the daily dose of adult beta glucan 3 to 100 mg of Ganoderma lucidum fruit body powder, 5 to 100 mg of microbial polysaccharide (betaglucan), soybean fermented extract powder (enzyme activity 13000FU / g) 3 to 200 mg, ginkgo biloba extract is 3 to 50 mg (1.7 to 25 mg in total ginkgo flavone glycosides), and the organolpi is more preferably added as 5 to 100 mg as psorigalpi. And it is good to add 1 to 100 mg of Rizveratrol, beta carotene is preferably 2 to 500 mg.

Experimental Example 7. Confirmation of side effect elimination effect (3)

One week of acne patients and one menopausal woman were used for two weeks, with Group 1 taking 0.5 g of commercial pomegranate extract (containing 50% extract) in the morning and evening, and Group 2 taking 20 mg of lycopene. In the case of acne, the severe condition before the experiment was set to 10 and completely cured was set to 0. The severe side effects were also set to 10 and no cases were set to 0. Acne test results are shown in Table 7 below.

TABLE 7

group  Treatment effect Degree of Side Effects Week 0 1 week 2 weeks Week 0 1 week 2 weeks Group 1 (acne) 10 9 8 0 4 7 Group 2 (acne) 10 8 6 0 One 2

As shown in Table 7, in the case of acne and menopausal women of Group 1, erythema appeared partially on the face as shown in Figure 1, but not so severe as when taking isoflavones. Lycopene, as in Group 2, was more effective in treating acne and had fewer side effects. However, lycopene alone showed little acne treatment. Menopausal women also had no spots and were much more effective for skin care.

Experimental Example 8. Confirmation of the concentration of pomegranate extract for the treatment of acne

Appropriate dose levels of pomegranate extract for acne patients were investigated. A daily dosage of pomegranate extract (containing 50% pomegranate extract) is 20, 50, 100, 200, 400, 600, 800, 1000, 2000, 3000, 4000 mg, divided into two concentrations each morning and evening. Intake with 20 mg of lycopene for 4 weeks. Each group is represented by pomegranate extract concentration. Before the experiment, the severe condition was set to 10 and completely treated as 0. The severe side effect was also set to 10 and no case was 0. The experimental results are shown in Table 8 below.

TABLE 8

Group (mg)  Treatment effect  Degree of Side Effects Week 0 2 weeks 4 Weeks Week 0 2 weeks 4 Weeks 20 0 8 7 0 0 0 50 0 8 6 0 0 0 100 0 8 6 0 0 One 200 0 7 5 0 One One 400 0 7 5 0 One One 600 0 6 5 0 One 2 800 0 6 4 0 2 2 1000 0 6 4 0 2 3 2000 0 6 3 0 3 4 3000 0 6 3 0 3 5 4000 0 3 - 0 6 -

In Table 8, the higher the pomegranate extract concentration, the better the acne treatment effect, but the higher the side effects. The daily dose of pomegranate extract in Table 8 and in further experiments ranged from 20 mg to 4 g, preferably from 20 mg to 3 g, based on adults.

Experimental Example 9. Confirmation of elimination of side effects (4)

In Experimental Example 7, when the pomegranate extract and lycopene were taken twice a day, there were those who showed lighter side effects compared with the administration of isoflavone and lycopene.

Pomegranate extract (containing 50% pomegranate extract) 600 mg + lycopene 40 mg added vitamin C 500 mg (group 1), vitamin E 400 IU addition (group 2), 0.3g squalene addition (group 3) It was taken for 4 weeks. Before the experiment, the severe condition was set to 10 and completely treated as 0. The severe side effect was also set to 10 and no case was 0. The experimental results are shown in Table 9 below.

TABLE 9

group  Treatment effect  Degree of Side Effects Week 0 2 weeks 4 Weeks Week 0 2 weeks 4 Weeks One 0 5 4 0 0 0 2 0 5 4 0 0 0 3 0 5 4 0 0 0

Looking at the above Table 9 was excellent in the acne treatment effect in all groups, yet little side effects. As shown in Table 9 above, there was no side effect (erythema) such as snorkel while all were in a therapeutic effect. In addition, in the case of B and C complex (composition example is the same as Experimental Example 5) instead of vitamin C of group 1, no adverse effects appeared. As a result of further experiments, these dosages are preferably 100 mg to 1 g of vitamin C, 100 to 1000 IU of vitamin E, and 0.1 to 2 g of squalene based on the daily dose of the adult. And it was better to add these vitamins and antioxidants to get rid of the side effects.

Experimental Example 10. Confirmation of elimination of side effects (5)

In reference to Experiment 9, acne patients received pomegranate extract (containing 50% pomegranate extract) 600 mg + lycopene (lycopene content 5%) 40 mg + 377 mg complex of vitamin B and C (composition, see Experiment 5). It is based on beta glucan, 20mg of Ganoderma lucidum extract (Group 1), 20 mg of soybean extract (Group 2), 20 g of Ginkgo biloba extract (Group 3), 10 mg of Lisveratrol (Group 4) Group 5), beta-carotene 20 mg (group 6) and isoflavones 20 mg (group 7) were added to each of the two groups, and divided into two times a day for 4 weeks. Their acne treatment and skin care effects are shown in Table 10 below.

TABLE 10

group  Treatment effect  Degree of Side Effects Week 0 2 weeks 4 Weeks Week 0 2 weeks 4 Weeks One 0 4 3 0 0 0 2 0 4 3 0 0 0 3 0 4 3 0 0 0 4 0 4 3 0 0 0 5 0 4 3 0 0 0 6 0 4 3 0 0 0 7 0 3 0 0 0 One

Looking at Table 10, it was shown that the acne treatment effect is excellent in all groups, and yet there are no side effects. However, adding isoflavones together in group 7 was effective for acne. But in terms of skin beauty, skin condition improved in order of 1> 2> 3> 4> 5> 6> 7. Therefore, beta glucan, soybean fermented extract, ginkgo biloba extract, ogalpi (gaciogalpi), lisveratrol, beta-carotene, isoflavones, etc. was better to add, and the mixed addition was also good in the future experiments. These additions were based on the daily dose of adult beta glucan 3-100 mg of Ganoderma lucidum fruit body powder, 5-100 mg of microorganism-producing polysaccharide (betaglucan), soybean fermented extract powder (enzyme activity 13000 FU / g) 3 to 200 mg, ginkgo biloba extract is 3 to 50 mg (1.7 to 25 mg in total ginkgo flavone glycosides), and the organolpi is more preferably added as 5 to 100 mg as psorigalpi. And it is good to add 1 to 100 mg of Rizveratrol, beta carotene is preferably 2 to 500 mg.

Experimental Example 11. Confirmation of effect according to phytoestrogens

A phytoestrogens-containing lotion was prepared and applied to the forehead for sebum inhibition. Inhibitory effects were naringin, naringenin, phloretin, quercetin, and biochanin A, but none were zealalenone or kaempferol. It was.

In this way, phytoestrogens with sebum suppression function were selected and each acne patient was treated with phytoestrogens such as naringin (group 1), naringin and lycopene (group 2), naringenin (group 3), naringenin and lycopene (group 4), and proretin (Group 5), Proretin and Lycopene (Group 6), Quercetin (Group 7), Quercetin and Lycopene (Group 8), Biochanin (Group 9), Biochanin + Lycopene (Group 10), and phytoestrogens are 1 People received 20 mg two weeks. At this time, lycopene (containing 5%) was taken with 40 mg. Before the experiment, the severe condition was set to 10 and completely treated as 0. The severe side effect was also set to 10 and no case was 0. The experimental results are shown in Table 11 below.

TABLE 11

group  Treatment effect  Degree of Side Effects Week 0 2 weeks Week 0 2 weeks Group 1 10 7 0 3 Group 2 10 5 0 0 Group 3 10 7 0 4 Group 4 10 5 0 0 Group 5 10 7 0 3 Group 6 10 5 0 0 Group 7 10 7 0 3 Group 8 10 5 0 0 Group 9 10 7 0 3 Group 10 10 5 0 0

As shown in Table 11, taking lycopene together was much more effective in the treatment of acne, with little side effects. It was also much more effective for skin care. Therefore, it was found that lycopene was effective to treat acne with the phytoestrogens in Experimental Example 11.

And it was better to add vitamin C, vitamin B and C complex, vitamin E, squalene as in Experimental Examples 5 and 9, the content was the same as the addition of Experimental Examples 5 and 9. In addition, it was good to add the Ganoderma lucidum extract, soybean fermentation extract, ginkgo biloba extract, lysveratrol, ogalpi, beta carotene as in Experimental Examples 6 and 10, and the amount was the same as in Experimental Example 10. Of course, the combined administration of phytoestrogens was also good. The daily dosage of naringin, naringenin, proretin, quercetin, and biochanin is preferably between 1 and 80 mg per adult.

Experimental Example 12. Confirmation of Skin Texture Change by Image Analysis

(1) skin color analysis

Soy isoflavones (30% total isoflavone content) 30 mg (group 1) per day, isoflavones 30 mg + lycopene (5% total lycopene content) 40 mg (group 2), pomegranate extract 0.4 g (group 3), pomegranate With 0.4 g of extract plus 40 mg of lycopene (group 4), two people each took groups 1 and 3 for two weeks in the morning and evening, and the faces were analyzed. Then I took a week off, then took groups 2 and 4 for 2 weeks each. The color of the facial skin was analyzed after taking with a skin visiometer. In other words, after taking group 1, the brightness of the forehead was input to the computer through the skin analyzer, and again after the group 2 was taken for 2 weeks, the change in facial brightness (skin whiteness) was shown.

The analysis results of Group 1 and Group 2 are in FIGS. 6 and 7. 6 is a case of group 1, and FIG. 7 is a case of group 2. FIG. The central point of FIG. 7 is 136, and in this respect, the skin of the light side is about 9% more than that of FIG.

In group 1, the skin became slightly cloudy compared to before taking the dose. And attempted to take lycopene only whitened similarly to group 2. Also, when comparing groups 3 and 4 experimented in the same way, it was better to add lycopene to pomegranate extract because it was similar to the results of groups 1 and 2.

As described above, the isoflavone and pomegranate extract itself tends to become cloudy, but when lycopene is added, the skin becomes white similarly to when lycopene is taken.

(2) analysis of skin texture

In the above experiments, the skin smoothness was measured using a skin visiometer. The ball was measured five times and the average value was shown. The results are shown in Table 12 below.

TABLE 12

Item LL R1 R2 R3 R4 R5 Before the experiment 430 58 42 33 10 32 Group 1 430 61 47 35 12 35 Group 2 430 53 40 30 7 28

Where R stands for "roughness" and generally means that the higher the value, the rougher. LL is the selected length of the skin, R1 is the distance between the highest and lowest depths of the skin, R2 is the average of R1 divided into 5 areas, R3 is the largest of the R1 values in 5 areas, and R4 is Average smoothness, R5 is average depth.

As can be seen from the above values, the skin became slightly rougher in Group 1 than before the experiment, and the skin texture was better in Group 2 than before the experiment. Thus, it can be seen that the skin is smoothed by adding lycopene to the isoflavone. When taking lycopene alone for 2 weeks, the 'R' value was about halfway between the pre-experiment value and the group 2 value.

Therefore, taking isoflavones and lycopene together showed that the skin became whiter and smoothest, similar to lycopene. And pomegranate extract showed a similar tendency. In future experiments, the addition of one or more of vitamin C, vitamin B and C complex, vitamin E, squalene, beta glucan, fermented soy extract, ginkgo biloba extract, beta carotene, ogalpi, and lizveratrol, It was better for skin texture.

Experimental Example 13. Confirmation of Lycopene Dose According to Isoflavone Dose for Menopausal Treatment

4 weeks menopausal women divided into morning and evening at each concentration of isoflavones (30% total isoflavone content) of 10 mg (group 1), 160 mg (group 2), and 200 mg (group 3) Each group was given two doses. At this time, lycopene (5% of total lycopene content) is administered in 1/3 (ri 1/3), equivalent (ri 1/1), and twice (ri 2/1) of the isoflavone dosage, but 200 mg is administered. It is not over. Severe side effects caused by isoflavones were set as 10 and no cases as 0. The experimental results are shown in Table 13.

TABLE 13

group   Degree of Side Effects Week 0 2 weeks 4 Weeks Group 1 0 One 2 Group 1-Lee 0 0 0 Group 1-Li 0 0 0 Group 1-Li2 / 1 0 0 0 Group 2 0 7 9 Group 2-L 0 2 3 Group 2-L 0 2 3 Group 2-L2 / 1 0 3 5 Group 3 0 8 10 Group 3-L 0 4 6 Group 3-L 0 4 6 Group 3-L2 / 1 0 5 7

As can be seen from Table 13, the group that did not take lycopene together became cloudy and rough with typical side effects. In addition, when taking isoflavones 160 and 200 mg, the erythema was severe and there was a rash, but when taken with lycopene, these side effects were alleviated.

Long experiments have shown that lycopene should be administered in the range of 5 to 200 mg for isoflavones, but the proper dose is about 1/2 of isoflavones. Lycopene 5 to 200 mg was also titrated against 1 to 80 mg of pomegranate extract from 2 mg to 4 g, genistein, genistin, glycitine, naringin, naringenin, proretin, quercetin, and biochanin. The concentration range was good.

Experimental Example 14. Confirmed acne and seborrheic dermatitis treatment

People with acne scars were selected and divided into two groups, control group (group 1), soy isoflavones (containing 30% total isoflavones) 25 mg (group 2), isoflavones 25 mg + lycopene (containing 5% lycopene) 60 mg (Group 3), Lycopene 60 mg (Group 4) was taken twice daily in the morning and in the evening.

In addition, people with seborrheic dermatitis marks were selected and divided as described above (groups 5 to 8), and taken in the morning and evening, respectively.

The severe condition before the experiment was set to 10 and completely treated as 0. The degree of rashes was confirmed as a side effect of isoflavones, and the results are shown in the following table.

TABLE 14

group  Treatment effect  Degree of Side Effects 0 days 10 days 20 days 0 days 10 days 20 days acne One 10 10 10 0 0 0 2 10 9 9 0 2 3 3 10 5 One 0 0 0 4 10 9 8 0 0 0 Dermatitis 5 10 10 10 0 0 0 6 10 9 9 0 2 3 7 10 6 2 0 0 0 8 10 9 8 0 0 0

As can be seen from the above table, there was no change of marks in Group 1 and Group 5, and Group 2 and Group 6 who took only isoflavones also had turbidity and rashes 3-4 with minimal effect of marks treatment. It wasn't as good as it appeared.

On the other hand, Group 3 and Group 7 have lost acne, seborrheic dermatitis marks and the skin is very good.

However, Group 4 and Group 8 seemed to have better skin, but most of the marks remained intact.

8 is a photograph of the group 3, by taking a combination of isoflavones and lycopene for 20 days, the face was very clean and acne marks disappeared.

As described above, the present invention is conspicuous and easily noticeable, and it is difficult to prolong the long-term use of acne treatment drugs, and it is possible to prevent and treat acne, seborrheic dermatitis and blemishes that are difficult to treat, and to treat premenstrual syndrome and menopausal disorders. You can relieve or eliminate many of the side effects that can occur with long-term or high doses.

In addition, the effect of removing acne marks or seborrheic dermatitis marks is not only useful as a skin care agent that can clean and smooth the skin, it can also be very useful in the pharmaceutical industry.

In addition, any combination of phytoestrogens, especially isoflavones or pomegranate extracts, may be provided with a mixed composition that may be taken with lycopene for the relief or elimination of side effects that may occur when taken alone.

Claims (8)

Mixed composition comprising lycopene and phytoestrogens, characterized in that orally administered by containing phytoestrogens and lycopene as an active ingredient. The method of claim 1, The phytoestrogens, a mixture comprising lycopene and phytoestrogens, characterized in that at least one or more substances from the group consisting of naringin, naringenin, proretin, quercetin, biochanin, pomegranate extract and isoflavones, alone or in combination Composition. The method of claim 1, Lycopene, characterized in that it further comprises at least one substance of vitamin C, vitamin B and C complex, vitamin E, squalene, beta glucan, soybean fermentation extract, ginkgo biloba extract, beta carotene, ogalpi (risgagalpi) or lizveratrol And a mixed composition comprising phytoestrogens. The method of claim 2, The isoflavone is a mixed composition comprising lycopene and phytoestrogens, characterized in that at least one selected from the group consisting of diezein, genistein, genistin and glycinein is used alone or in combination. The method of claim 1, Lycopene (lycopene content 5%) is a mixed composition comprising lycopene and phytoestrogens, characterized in that configured to 5 to 200 mg based on the daily dose of an adult. The method of claim 2, The isoflavone (isoflavone content 30%) is a mixed composition comprising lycopene and phytoestrogens, characterized in that configured to 5 to 200 mg based on the daily dose of the adult. The method of claim 2, The pomegranate extract (containing 50% pomegranate extract) is a mixed composition comprising lycopene and phytoestrogens, characterized in that it is configured to 20 mg to 4 g on an adult's daily dose basis. The method of claim 2, The naringin, naringenin, proretin, quercetin and biochanin is a mixed composition comprising lycopene and phytoestrogens, characterized in that configured to 1 to 80 mg based on the daily dose of the adult.

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