KR20060032733A - Functional composition comprising oldenlandiae herba and ganoderma lucidum extracts - Google Patents

Abstract

The present invention relates to a composition comprising baekhwasaeng and Ganoderma lucidum extract exhibiting antiviral activity, and more particularly, excellent antiviral activity against PMWS virus, PRRS virus, PED virus, TGE virus, parvovirus and distemper virus. It relates to a functional composition containing baekhwasacho and Ganoderma lucidum extract as an active ingredient.

The composition according to the present invention is a substance derived from natural products, has no toxicity and side effects, and exhibits excellent bactericidal effect against various viruses, and thus may be usefully used as an effective and safe food or feed composition for the prevention and improvement of viral diseases.

White flower, ganoderma lucidum mushroom, virus, medicine

Description

Functional Composition Comprising Oldenlandiae Herba and Ganoderma Lucidum Extracts

The present invention relates to a composition comprising baekhwasaeng and Ganoderma lucidum extract exhibiting antiviral activity, and more particularly, excellent antiviral activity against PMWS virus, PRRS virus, PED virus, TGE virus, parvovirus and distemper virus. It relates to a functional composition containing baekhwasacho and Ganoderma lucidum extract as an active ingredient.

As modern medicine advances, bacterial diseases are markedly suppressed by the development of antibiotics and preventive vaccines, but viral diseases are not yet conquered, but rather are reported to increase through air pollution and environmental pollution.

PMWS is a post-weaning multisystemic wasting syndrome of major weaning pigs.The main symptoms are appetite deficiency, systemic weakness, dyspnea, jaundice due to weight loss, and sometimes diarrhea, cough, and sometimes central nervous system disorders. Practically, it is a wasting disease characterized by multiple lesions in the general lymphatic organs. At the time of autopsy of infected pigs, systemic lymph node swelling and lymph node bleeding can be found. In these cases, morbidity is low in weaning pigs, but mortality is high (70%) in clinical cases (Harding, JC, Proc. West. Can.Assoc. Swine. Pract. , 58: 503, 1996). Although the exact cause of PMWS is still controversial, PCV2 is recognized as the primary cause of PMWS because the infection of porcine circovirus type 2 (PCV2) has been common in PMWS. Recently, in addition to PMWS, various disease syndromes such as reproductive disorders, swine dermatitis syndrome (PDNS), swine respiratory disease complex infection (PRDC), proliferative necrotic pneumonia (PNP), congenital tremors, etc. It has been reported that PCV2 is associated with disease.

Porcine reproductive and respiratory syndrome (PRRS) is caused by an enveloped positive-stranded virus with a membrane from the arteririviridae. About 10 to 15 years ago, two other PRRS virus strains were apparently independently present in the United States and Europe, and the disease now causes swine fertility and respiratory disease in many swine producing countries in North America, Europe and Asia. The prevalence of infection in the United States is estimated at 70% (Snijder, EF and Meulenberg JJM, J. of General Virology , 79: 961, 1998). The PRRS virus is infected by breathing, achievement, bite wounds or needles, and multiplies in the macrophage of the mucous membranes, lungs or local areas. Subclinically, the disease results in degradation or persistent infection, and persistently infected animals release the virus from oral / pharyngeal fluid, blood, feces, urine and semen. Clinical symptoms in sows are associated with preterm delivery of aborted or weakly born pigs, stillbirth pigs, and autolyzed fetuses.

The causative agent of porcine ephidemic diarrhea (PED) is the swine epidemic diarrhea virus belonging to the coronavirus, which is a coronavirus that is not antigenically related to the TGE virus. The virus propagates in epithelial cells of the small intestine and colon and mesenteric lymph nodes. PED is mainly generated in Europe and Japan, and has been steadily occurring since the PED virus was isolated and reported for the first time in 1992. The PED virus is combined with some transmissible gastroententis (TGE) to increase the damage, and is caused by sows of pigs regardless of age. Symptoms in mammalian pigs are vomiting and suddenly cause watery diarrhea, and they die. Adult pigs, fosters, and pigs recover sporadically from vomiting, diarrhea, and loss of appetite. Mortality is higher at younger age, and mortality of young pigs is rare. The pathogen of porcine infectious gastroenteritis (TGE) is a TGE virus belonging to the coronavirus and is easily killed by sunlight, high temperature and is easily inactivated in formalin, caustic soda, iodine solution, ether and chloroform. The development of TGE is almost similar to that of PED, but it does not occur in hot seasons, but mainly in winter.

Canine parvovirus (CPV) belongs to the genus parvovirus (pravoviridae), parvovirus (parvovirus), and belongs to the feline parvovirus (feline parvovirus). Canine parvopyrus particles are 18-26 nm in diameter, without envelopes and are single-stranded DNA viruses, and viral proteins contain three polypeptides. The main symptoms caused by parvovirus are severe after 5 days of oral infection. When susceptible dogs are exposed to the virus, 100% of the dogs are infected, about 75% of them are non-infectious, and 25% have high mortality. Early symptoms include lack of energy, loss of appetite and vomiting. Diarrhea is observed within 24 to 48 hours after vomiting begins, and diarrhea, including dehydration, weight loss, emulsification, and blood, is observed if progression continues.

Carnine distemper virus infection is a highly infectious acute or subacute recessive disease in dogs and other predators. It is not only found in dogs worldwide, but also in dogs such as dogs, foxes, wolves, and raccoons. Raccoons, pandas and other weasels, such as weasels, badgers, skunks, and ferrets, are infected by carnivores and are a viral disease with high morbidity and mortality. The pathogen of canine distempervirus infection is a distemper virus and belongs to the parabilliovirus of paramyxoviridae, which is contained in large amounts of blood, cerebrospinal fluid, lymph nodes, spleen, lungs, bronchus, and brain in congested blood. In acute cases, it is discharged into droplets, eye secretions, saliva, urine, etc., and is spread to airway infections and contact infections. Clinical symptoms vary, but usually 4 symptoms such as respiratory, digestive, skin and nerves appear alone or in combination. Respiratory symptoms such as fever, nasal secretion, cardinal aneurysm, bronchitis, pharyngitis and diarrhea are also present. There are few treatments for canine distempervirus infection, and serum preparations are sometimes used as serum therapy, but they are not practical because they are easy to miss.

The PMWS, PRRS, PED, TGE, CD and CP viruses, etc. are occurring all over the world, giving a huge economic loss in the livestock industry. However, it is urgent to find a proper countermeasure for this, and therefore, an urgent measure is required.

On the other hand, bleaching saseolcho of the present invention is a high as 20 ~ 40㎝ annual sentinel of the one year old herbal baekunpul (Hedyotis lindleyana var. Hirsuta HARA or Oldenlandia diffusa L. ROXB.) Belonging to the Rubiaceae (Rubiaceae), to the side the bottom of the As it grows, the branches split and stand at an angle, with a few multicellular white hairs (Yoshida, Y. et al., Int. J. Immunopharmacol. , 19: 359, 1997). The blemishes of Baekhwasaolcho are not cold or poisonous, the taste is sweet and bitter, and the meridians that act are stomach, large, and small intestines.瘀) It is effective in swelling of pneumonia, tonsillitis, sore throat, appendicitis, dysentery, jaundice, uterine appendixitis, arthritis, and digestive cancer, liver cancer, lung cancer, lymph cancer and throat cancer. (Tong, IH et al., Phytochemistry. , 25: 1988, 1986).

Furthermore, Ganoderma lucidum (. Ganoderma lucidum (Fr) Karst ) belongs to bulrochogwa (Ganodermataceae), called the bulrocho, mushrooms ten thousand years, is found in temperate minutes north hemisphere, including South Korea, Japan and China. It is a root-bearing fungi that grows or grows on hardwoods or stumps of broad-leaved trees from summer to autumn. It contains various amino acids, water-soluble proteins, steroids, anthraquinone derivatives, carbohydrates, various glycosides, small amounts of alkaloid-reducing substances and lignin. Contains enzymes that degrade Ganoderma lucidum has no side effects, and is widely used for the treatment and prevention of various kinds of diseases such as anticancer effect, squeezing effect, anti-blood effect, glycemic effect, diuretic effect, detoxification effect and widely used for medicinal purposes Polysaccharides are recognized as bioreactive modulators that can confer nonspecific active immunity to tumors (Shimura, K. et al., Japan. J. Pharmac. , 33: 403, 1983). In addition, the polysaccharide extracted and separated from the Ganoderma lucidum mycelium culture is reported to have antimicrobial activity and anticancer activity by directing cytotoxicity against microorganisms and tumor cells and enhancing immune function of the host according to macrophage activity. (Kim, SW, J. Korean. Soc. Food. Sci. Nutr. , 27: 1183, 1998).

Derivation of the biologically active substance based on the natural product as described above is economical as well as less side effects and toxicity, research has been actively conducted recently. As a virus therapeutic agent using herbal medicines, Korean Patent Application No. 10-2003-0011238 discloses antiviral activity against hepatitis B virus (HBV) and human immunodeficiency virus (HIV) of African Ganoderma lucidum extract, Korean Patent Application No. 10-2003-0024419 discloses a pharmaceutical composition for treating HIV / AIDS patients containing chlorosis. However, no disclosure or teaching has been made on the antiviral activity of P. vulgaris and Ganoderma lucidum extracts against various viruses such as PMWS virus, PRRS virus, PED virus.

The inventors of the present invention, while studying the antiviral activity against a variety of viruses in the herbal medicines derived from natural products with little toxicity and side effects, PMWS virus, PRRS virus, PED virus, TGE virus Excellent antiviral effects on the CP virus and CD virus were confirmed and the present invention was completed.

After all, the main object of the present invention is to provide a feed composition for the prevention or improvement of viral diseases containing baekhsasacho and ganoderma lucidum extract as an active ingredient.

Another object of the present invention is to provide a food composition for preventing or ameliorating a viral disease, including the baekrye-saengcho and Ganoderma lucidum extract and a food supplement.

In order to achieve the above object, the present invention provides a feed composition for the prevention or amelioration of viral diseases, including the extract of oldenlandia herba and ganoderma lucidum having antiviral activity.

The present invention also provides a food composition for the prevention or amelioration of a viral disease, which contains antibacterial activity of baekrye-saengcho and ganoderma lucidum extract and a food acceptable additive.

In the present invention, the baekrye ssaengcho and Ganoderma lucidum extract may be blended in a weight ratio of 0.1-10: 0.1-5, preferably in a weight ratio of 4-10: 1-3, more preferably 7-9: It may be formulated in a weight ratio of 1 to 3, the baekrye sagecho and ganoderma lucidum extract may be characterized in that it comprises 10 to 99% by weight based on the total weight of the composition.

In the present invention, the composition is chaga, situation mushroom, fingerling mushroom, Cordyceps sinensis, roe beet mushroom, Bokyeong, Agarikusu, longevity mushroom, leafy mushroom, spirit, horseshoe mushroom, oyster mushroom, enoki mushroom, shiitake mushroom, spirit It may be characterized by further comprising one or more herbal extracts or sodium dichloroisocyanurate selected from the group consisting of mushrooms, mulberry mushrooms, tile mushrooms, pine mushrooms and sour mushroom extracts.

In the present invention, the virus is a post-weaning multisystemic wasting syndrome (PMWS) virus, a porcine reproductive and respiratory syndrome (PRRS) virus, a porcine ephidemic diarrhea (PED) virus, a transmissible gastroententis (TGE) virus, a carnine parvo (CP) virus. Or a CD (carnine distemper) virus.

In the present invention, the feed composition may be characterized by feeding to pigs, dogs, foxes, wolves, raccoons, pandas, weasels, badgers or skunks.

Hereinafter, the present invention will be described in detail.

Baeksasaeng and Ganoderma lucidum extract of the present invention can be obtained as follows.

Purified white sesame seed or Ganoderma lucidum mushroom and pulverized to 30-50 mesh, water and methanol of about 2 to 10 times, preferably about 3 to 6 times the weight of the whitening saecho or ganoderma lucidum sample, A polar solvent of C ₁ to C ₄ lower alcohols such as ethanol, butanol, or the like, or a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10, preferably about 1 to about 70% ethanol or alcohol at room temperature. After extraction using hot water extraction, cold needle extraction, reflux cooling extraction or ultrasonic extraction for 1-5 weeks, preferably 1-5 days, and then filtered with a filter of 0.1 ~ 10㎛, and then the supernatant Gathered and concentrated under reduced pressure to obtain 10 ~ 70 brix, preferably 20 ~ 50 briquettes of baekryeoksa or ganoderma lucidum extract.

Baeksasulcho and ganoderma lucidum mushroom extracts can be obtained by extracting each in the same manner as described above, then Baeksasasulgo and ganoderma lucidum extract, or by mixing each component first, followed by extraction according to the above method.

In addition, the non-polar solvent soluble extract of the present invention is suspended by distilled water, the suspension is added to a nonpolar solvent such as hexane, ethyl acetate, chloroform of about 1 to 100 times, preferably about 1 to 5 times the volume 1 It can be obtained by extracting and separating the non-polar solvent soluble layer once to 10 times, preferably 2 to 5 times. It is also possible to further carry out conventional fractionation processes (Harborne, JB, Phytochemical methods: A guide to modern techniques of plant analysis, 3: 6-7, 1998).

The present invention provides a functional composition for the prevention and improvement of a viral disease containing baekhsasacho and ganoderma lucidum extract obtained by the above method as an active ingredient. The composition of the present invention may further include any other herbal medicine or extract thereof to enhance the effect, in this case, after preparing the extract of the herbal medicine according to the extraction method, or added to the composition of the present invention And after mixing the Ganoderma lucidum and the herbal medicine together, the extract obtained by the extraction by the above method can be included in the composition.

Herbal extracts that can be added to the composition of the present invention include chaga, situation mushroom, fingerling mushroom, Cordyceps sinensis, roe deer day mushroom, fueryeong, agaricus, longevity mushroom, leaf mushroom, white neck mushroom, herb, horseshoe mushroom, oyster mushroom , Mushrooms, mushrooms, shiitake mushrooms, mulberry mushrooms, tiled mushrooms, pine mushrooms and sour mushrooms extract may be exemplified, but is not limited thereto, the herbal extracts may be used alone or in combination with each other. In addition, it may further include sodium dichloroisocyanu rate (sodium dichloroisocyanu rate) to enhance the effect of the composition of the present invention. The added herbal medicine extract and sodium isocyanate dichloride may be included in an amount of 0 to 90% by weight, preferably 40 to 80% by weight, based on the total weight of the composition.

In addition, the baekhwasacho and ganoderma lucidum of the present invention has been used as a herbal medicine for a long time, the extracts of the present invention extracted therefrom also have no problems such as toxicity and side effects.

As a result of searching the antiviral effect of the extract of P. vulgaris and Ganoderma lucidum extract obtained by the above method, it was confirmed that the antiviral activity was excellent against PMWS virus, PRRS virus, PED virus, TGE virus, CP virus and CD virus. .

The composition containing baekrye sagecho and ganoderma lucidum extract having the antiviral activity of the present invention can be usefully used as a feed composition or food composition for the prevention and improvement of viral diseases.

Baeksasaeng and Ganoderma lucidum extract of the present invention can be added to various foods for the prevention of viral diseases. Examples of the food to which the extract can be added include various foods, beverages, gums, teas, vitamin complexes, and health functional foods.

The health functional beverage composition of the present invention is not particularly limited to other ingredients except for having the extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates, etc. as additional ingredients, as in general beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

In addition to the above, the extract of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and its Salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the extracts of the present invention may contain flesh for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is usually selected in the range of 0 to 20 parts by weight per 100 parts by weight of the extract of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1: Preparation of Baekhwa Jijisan

1-1. Preparation of Pear Blossom

After purchasing dried white chlorosis, it was pulverized into 30-50 mesh, 10 ml of 40% ethanol was added to 1 g of chlorosis, and then refluxed at 100 ° C. for 3 hours, and then the liquid component was 35 brix (brix). Evaporated to give chlorosis extract.

1-2. Preparation of Ganoderma lucidum extract

After purchasing dried Ganoderma lucidum mushrooms, crushed into 30-50 mesh, 10 ml of 40% ethanol was added to 1 g of white sand vinegar powder, followed by reflux extraction at 100 ° C. for 3 hours, and then evaporated the liquid component to 35 bricks. Ganoderma lucidum mushroom extract was then obtained.

1-3. Preparation of Baekhwa Youngjisan Extract

After purchasing dried white saengchocho and ganoderma lucidum mushrooms, crushed into 30-50 mesh, mixed with crushed whitehachosulcho powder and ganoderma lucidum powder in a ratio of 8: 2, and extracted by the same method as above. A mixed extract of the mushroom was obtained, which was named 'baekhwa youngjisan'.

Example 2: PMWS virus sterilizing effect

In order to examine the bactericidal effect of Baek Young Ji-san, the causative agent of PMWS was PK 15 cell line as a porcine circovirus type 2 (PCV2) cell line, and PCV2 strain DR673 (10 ^6.0) as a positive control virus. TCID ₅₀ /0.1 mL by PCR) was used.

After adding 1 ml of white chlorosis acid and 1 ml of virus solution, sterilized hard water was added to make 8 ml, and the mixture was diluted 1:10 times. 1 ml of the virus solution was added to 1 ml of the diluted solution, sterilized hard water was added to make 8 ml, and the mixture was diluted 1:50 times. The same procedure was repeated to obtain dilutions of 500, 800, 1,000, 1,200, 1,500, and 2,000 times. The resulting dilutions were incubated at 4 ° C. for 30 minutes, and three 96-well plates containing PCV from which PK-15 cells had been removed were removed from the medium and washed with PBS. 100 μl of each dilution (800, 1000, 1200, 1500, 2000 times) was inoculated three times in a 96-well plate, followed by incubation at 37 ° C. for 1 hour, and the negative control group received 100 μl of the culture medium. . After incubation, the dilutions were removed and washed three times with PBS. After incubating 50 μl of 300 mM D-glucosamine in each well of the plate, the culture solution was incubated for 15 minutes, and then the virus solution was removed and washed three times with PBS. 200 μl of the culture medium was added to each well of the plate from which the virus solution was removed, followed by further incubation for 48 hours at 37 ° C., and then the bactericidal effect against PMWS virus was measured using the IFA method. That is, after removing the culture medium from each well, a 96-well plate was fixed using ethanol for 2 hours or more at -20 ° C, and then ethanol was removed and the plate was dried for 30 minutes. 100 μl of PCV positive serum was added to each well, incubated for 2 hours at 37 ° C., and then serum was removed, washed three times with 100 μl of PBS, followed by 50 μl fluorescein conjugated anti-pig IgG ( Fluorescein conjugated anti-swine IgG) was added to each well and incubated at 37 ° C. for 1 hour. Each well was washed once again and the plates were observed using a fluorescence microscope (10X objective lens).

As a result, it was confirmed that Baek Young Jisan of the present invention effectively sterilizes PCV2 virus to 1,200-fold concentration under 1% organic matter and 5% organic matter conditions (see Table 1).

1% organic matter 5% organics 800 U U 1,000 U U 1,200 Null Null 1,500 Null Null 2,000 Null Null

Example 3: Antiviral Effect

To measure the PRRS, PED, TGE, CP and CD virus bactericidal effects of Baek Young Jisan, the cell lines and the published virus of Table 2 were used. The experiment on the virus disinfection effect of Baekhwa Youngjisan was carried out according to the guidelines for testing the disinfectant efficacy of viruses (Korean Animal Drug Association: http://kahpa.or.kr/index_k.htm, prescribed in Annex 2) as follows.

Specifically, MEM [minimum essential medium; 10% FBS to the cell line for each virus; L-glutamine, HEPES buffer, non-essential amino acids, NaHCO ₃ , antibiotics] media were used for subculture, using live virus during subculture, and harvesting at the point of maximum virus proliferation and shortly before use. And stored in ice water for a while. Before reacting the test sample and virus, the samples were diluted 400 times, 500 times, 600 times, 700 times, 800 times, 900 times, and 1000 times according to the dilution method of the disinfectant in the disinfectant test guide. 1.0 ml of the expanded 4 ° C. virus solution was mixed with 19.0 ml of 1% organic matter dilution at 4 ° C., 2.5 ml of the mixed solution was removed at 1 minute intervals, and mixed in a test tube containing the same amount of sample dilution at 4 ° C. The reaction was carried out for exactly 30 minutes at 4 ℃, was mixed every 10 minutes in the middle of the reaction. The control group used hard or distilled water instead of the sample. After the reaction was completed, 1.0 ml was immediately taken out, mixed in a neutralizing medium of 37 ° C. in the same amount, and then diluted. The neutralization reaction solution was diluted and the appropriate amount was inoculated into cells to measure the virus content. To determine the proliferation of the virus, the medium was removed from the monolayer plate to be inoculated with the sample, washed with PBS, and inoculated three times with 100 µl of the diluted sample in each well of the plate. At this time, the negative control group was added 200µl of the media component, the plate was incubated for 72 hours at 37 ℃, and observed the growth of the virus using the cytopathic effect (CPE). The Kaerber method was used for the virus content. The virus concentration of the pathogen control group was compared with the virus content of the sample treatment group, and the dilution concentration for which 10 ⁴ TCID ₅₀ inactivation was recognized was used as an effective concentration. The median of the results was taken.

As a result, as shown in Table 3, the white baekryongji of the present invention was confirmed to effectively sterilize the PRRS virus 1: 1 concentration in 5% organic matter conditions. In addition, it was confirmed that PED virus, TGE virus and canine parvovirus (CPV) were effectively sterilized to a concentration of 1: 800 and canine distemper virus (CDV) to a concentration of 1: 500.

Cell line Disclosure Virus PRRS virus MARC-145 cells VR-2332 strain, 10 ^5.0 TCID ₅₀ / ml PED virus Vero cells KPEDV-9 strain, 10 ^5.5 TCID ₅₀ / mL TGE virus ST cells Pyungtak-40 strain, 10 ^5.5 TCID ₅₀ / ml CP virus (canine parvovirus) CRFK cells C-780916 strain, 10 ^5.0 TCID ₅₀ / mL CD Virus (Dog Distempervirus) Vero cells Lederle strain, 10 ^5.0 TCID ₅₀ / ml

Dilution factor Antiviral Effective Concentration PRRS PED TGE CP CD 1: 400 U U U U U 1: 500 U U U U U 1: 600 U U U U radish 1: 700 U U U U radish 1: 800 U U U U radish 1: 900 U radish radish radish radish 1: 1,000 radish radish radish radish radish

Example 4: Toxicity Assessment

Toxicity experiments were conducted using three week-old Sprague-dawley rats, 50 males each. First, the experimental animals were purified for 1 week at a temperature of 22 ± 3 ° C., a relative humidity of 50 ± 10%, and a 12 hour contrast chamber, and then screened for general symptoms to select only healthy rats (cancer, 40 for each). Randomly, they were classified into a solvent control group, a low dose administration group [feed 0.0001% (w / w)], a medium dose administration group [feed 0.01% (w / w)], and a high dose administration group [feed 0.1% (w / w)]. The powder of P. sulciosa extract of the present invention was formulated with a feed powder and starch according to the concentration of the test substance, and then fed freely to 10 animals of each male and female. The test substance was administered once a day for a total of 90 days. After administration of the test substance, mortality, clinical symptoms and changes in body weight were observed, hematological and serum biochemical tests were performed. Was measured and histopathological examination was performed.

As a result, no significant clinical symptoms or dead animals were noted in all animals treated with the test substance, and no toxic changes were observed in weight changes, blood tests, blood biochemical tests, and autopsy findings. As a result, baekrye saengcho of the present invention did not show a change in toxicity at a concentration of less than 10% feed intake of baekrye-saengcho extract in high doses, and was determined to be a safe substance with a minimum lethal dose (LD ₅₀ ) of about 5731 mg / kg. .

Having described the specific part of the present invention in detail, it is obvious to those skilled in the art that such a specific description is only a preferred embodiment, thereby not limiting the scope of the present invention. something to do. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

As described in detail above, the composition comprising the baekhwasasaeng and Ganoderma lucidum extract of the present invention has no toxicity and side effects as a substance derived from natural products, and shows an excellent bactericidal effect against various viruses, thereby preventing and improving viral diseases. It can be usefully used as an effective and safe food or feed composition.

Claims (10)

Feed composition for the prevention or amelioration of viral diseases comprising an extract of oldenlandia herba and ganoderma lucidum having antiviral activity. The composition according to claim 1, wherein the whitening sandweed and Ganoderma lucidum extract are blended in a weight ratio of 0.1-10: 0.1-5. The method of claim 1, wherein the composition is chaga, situation mushroom, fingerling mushroom, Cordyceps sinensis, roe deer fungus, Fueryeong, Agarikusu, longevity mushroom, leafy mushroom, herb, horseshoe mushroom, oyster mushroom, enoki mushroom, shiitake mushroom, Composition comprising a further one or more herbal extracts or sodium dichloroisocyanurate selected from the group consisting of the mushroom, mulberry, tile mushroom, matsutake and sour mushroom extract. According to claim 1, wherein the virus is post-weaning multisystemic wasting syndrome (PMWS) virus, porcine reproductive and respiratory syndrome (PRRS) virus, porcine ephidemic diarrhea (PED) virus, transmissible gastroententis (TGE) virus, carnine parvo (CP) A composition comprising a virus or a CD (carnine distemper) virus. The composition of claim 1, wherein the feed composition is fed to pigs, dogs, foxes, wolves, raccoons, pandas, weasels, badgers or skunks. A food composition for preventing or ameliorating a viral disease, comprising baekhwasacho and ganoderma lucidum extract, and a food-acceptable food supplement additive having an antiviral activity of claim 1. 7. A composition according to claim 6, formulated in the form of a powder, tablet, granule, capsule or beverage. The composition of claim 6, wherein the baekhwasaengcho and Ganoderma lucidum extract are blended in a weight ratio of 0.1-10: 0.1-5. The method of claim 6, wherein the composition is chaga, situation mushroom, fingerling mushroom, Cordyceps sinensis, roe deer fungus, Fueryeong, Agarikusu, longevity mushroom, leafy mushroom, herb, horseshoe mushroom, oyster mushroom, enoki mushroom, shiitake mushroom, Composition comprising a further one or more herbal extracts or sodium dichloroisocyanurate selected from the group consisting of the mushroom, mulberry, tile mushroom, matsutake and sour mushroom extract. The virus of claim 6, wherein the virus is a post-weaning multisystemic wasting syndrome (PMWS) virus, a porcine reproductive and respiratory syndrome (PRRS) virus, a porcine ephidemic diarrhea (PED) virus, a transmissible gastroententis (TGE) virus, or a carnine parvo (CP) virus. A composition comprising a virus or a CD (carnine distemper) virus.