KR20050028272A - Functional food prepared from combined prescription having an effects on diet, diabetes, hyperlipemia and radical scavenging - Google Patents

Abstract

Functional food containing medicinal herbs is provided, which functional food has effects on diet, anti-diabetes, anti-hyperlipemia and radical scavenging. A diet, anti-diabetic, anti-hyperlipemia and radical scavenging composition comprises 5 to 15 wt.% of Bombyx mori L., 5 to 15 wt.% of ginseng tail, 5 to 15 wt.% of Nelumbo nucifera Gaertner, 5 to 15 wt.% of root joint of Nelumbo nucifera Gaertner, 5 to 15 wt.% of Luffa cylindrica Roem, 5 to 15 wt.% of Citrus reticulata seeds, 5 to 15 wt.% of Laminaria, 5 to 15 wt.% of Platycodon grandiflorum and 5 to 15 wt.% of Lonicera japonica THUNB.

Description

Functional Food Prepared from Combined Prescription Having an Effects on Diet, Diabetes, Hyperlipemia and Radical Scavenging}

The present invention relates to a functional food effective for diet, antidiabetic, hyperlipidemia and antioxidant. More specifically, the present invention includes a herbal medicine including white asleep, rice bran, lotus root, falcon, apple rock, mandarin kernels, bales, gilyeong and gilding to lower blood glucose, triglyceride and cholesterol values. The present invention relates to a functional food that is effective in diet, antidiabetic, hyperlipidemia and antioxidant by compounding the ingredients.

Although economic development and cultural benefits are enjoyed by the development of modern industrial society, our lives are directly or indirectly threatened by environmental pollution or over-nutrition, and in response, modern people are concerned about their health. More than ever, there is a growing interest in functional foods or such drugs that are effective in maintaining health or regulating biorhythms.

On the other hand, in the function of food until recently, the function of nutrition and its taste or tissues on the depreciation of the human body, etc., but the range of the function of food currently is to neutralize, detox, excrete, blood pressure, blood sugar, cholesterol, It is developing into a functional food having bioregulatory functions such as obesity prevention and diet.

Functional food refers to foods that have bioregulatory functions such as biological defense, immunity, prevention and treatment of diseases, recovery of symptoms, and inhibition of aging, and should be harmless to the human body when taken in the long term.

Currently, the most problematic diseases of most adult diseases are circulatory diseases and diabetes caused by obesity due to overnutrition and lack of exercise. The most preferable way to prevent and treat this is to prevent diseases in advance by using functional foods, and functional foods that can be used for the treatment and prevention of them are organza, tofu, cornus and omija, which have been used as nutrient tonics since ancient times. , Wolfberry, ginseng and gourd.

To prevent diabetes, you should not consume too much carbohydrates first, and it should not be absorbed even if you eat it. There may be a variety of foods that have such an effect, but currently, baekgamjang, samsam, yeonjak, pilgrim, apple rock, mandarin kernels, bales, and Gilkyung are known through active experiments and herbal studies.

The effect of these plants is known that the extract acts on the intestine, inhibiting or absorbing the absorption of sugar, triglycerides and cholesterol, which effectively reduces the amount of blood sugar, triglycerides and cholesterol.

As mentioned above, the biggest problem of modern people is a disease such as diabetes due to obesity, and in order to prevent or treat this disease, it is most ideal to inhibit the absorption of the carbohydrates in the small intestine when the carbohydrate is eaten. In Korea, only extracts such as bundling or red ginseng are sold as functional foods.However, by using a single herbal medicine, not only certain functions can be expected by a single herbal medicine, but also the disadvantages of a single herbal medicine cannot be secured. There was this.

Accordingly, the present invention is provided to more efficiently solve the problems of the prior art as described above,

An object of the present invention is a combination of herbal ingredients consisting of baekpja, misam, lotus, chops, apple rock, mandarin kernels, bales, gilyeong and gold and silver coin to lower blood glucose, triglyceride and cholesterol (cholesterol) values The prescription is to provide a functional food effective for diet, antidiabetic, hyperlipidemia and antioxidants.

The above and other objects can be achieved by the present invention described below.

In order to achieve the above object, the diet, antidiabetic, hyperlipidemia and antioxidant composition according to the present invention is 5 to 15% by weight, 5 to 15% by weight, 5 to 15% by weight, 5 to 15% by weight of scapula 5 to 15% by weight, 5 to 15% by weight, 5 to 15% by weight, 5 to 15% by weight, 5 to 15% by weight, and 5 to 15% by weight of gold coins.

Juice and flavoring may be further included in the composition as an additive.

Hereinafter, the present invention will be described in detail.

In the present invention, herbal medicines that act on the carbohydrates and fats in the small intestine, inhibit absorption and are also absorbed to lower blood glucose, triglycerides and cholesterol levels. Preferred herbal medicines of the present invention include Baekgamjang, misam, lotus root, pilgrim, apple rock, tangerine core, packing, Gilkyung, gold and silver coins.

Baekgamjang is a fungus in which silkworm larvae stiffen with leukemia caused by infection with Leukemia bacteria. It lowers blood sugar and has a therapeutic effect for acute mastitis. Baekjang according to the present invention is used in 5 to 15% by weight, more preferably 10 to 13% by weight. When it is used below 5% by weight, it is difficult to expect the medicinal effect.When it is used above 15% by weight, it is not supplemented with other herbal ingredients.

Misam refers to the roots of ginseng, which is cheap and can get the effect of ginseng, and is generally used a lot. Misam is known to be effective in fatigue recovery, physical strength enhancement, and blood pressure enhancement. Misam according to the present invention is used 5 to 15% by weight, more preferably 10 to 13% by weight. When it is used below 5% by weight, it is difficult to expect the medicinal effect.When it is used above 15% by weight, it is not supplemented with other herbal ingredients.

The fruit of lotus is called Yeonjakyuk (蓮 子 肉), if you have a large boil and drink to see the effect, overall tonic, nourishing action is excellent for postpartum women. There is a function to clear the heart (廳) can be used to try insomnia or heart disease (心悸). Soft meat according to the present invention is used in 5 to 15% by weight, more preferably 10 to 13% by weight. When it is used below 5% by weight, it is difficult to expect the medicinal effect.When it is used above 15% by weight, it is not supplemented with other herbal ingredients.

The root of the lotus root is called 'wool' in oriental medicine, and it is not only good for various bleeding diseases, anemia, etc., but also has an excellent gangjeong effect, as well as excellent effect on the non-gastrointestinal digestive system. The falcon according to the present invention is used at 5 to 15% by weight, more preferably at 10 to 13% by weight. When it is used below 5% by weight, it is difficult to expect the medicinal effect.When it is used above 15% by weight, it is not supplemented with other herbal ingredients.

Apple-lac, also known as loofah, is known to have a good effect on the stalk of swelling and flatulence. Apple Lac according to the present invention is used in 5 to 15% by weight, more preferably in 10 to 13 weight percent. When it is used below 5% by weight, it is difficult to expect the medicinal effect.When it is used above 15% by weight, it is not supplemented with other herbal ingredients.

Tangerine nucleus is a seed of the tangerine tree and has pharmacological properties effective for testicles, mastitis and back pain. Tangerine core according to the present invention is used in 5 to 15% by weight, more preferably in 10 to 13% by weight. When it is used below 5% by weight, it is difficult to expect the medicinal effect.When it is used above 15% by weight, it is not supplemented with other herbal ingredients.

Packing is a low-calorie, mineral-rich alkaline food, rich in calcium, strong bones, and lowers blood pressure (laminin), which is good for high blood pressure and has anticancer activity. There is an action to improve the thyroid dysfunction and hyperactivity, and remove the blood, and hemorrhoidal hemorrhagic hemorrhagic artery bleeding and fever and diuretic action, the packing according to the present invention is used in 5 to 15% by weight, more preferably Is used in 10 to 13% by weight. When it is used below 5% by weight, it is difficult to expect the medicinal effect.When it is used above 15% by weight, it is not supplemented with other herbal ingredients.

Gilkyung is known as a herbal medicine that has been used in respiratory diseases such as drainage, expectoration, bronchitis, asthma and anti-inflammatory. The main pharmacological components of Gilkyung are terpene-based saponins, hemolysis, local stimulation, antitussive action, expectoration, central nervous system depression, anti-inflammatory action against acute inflammation, anti-allergic, anti-ulcer and gastric juice secretion, blood vessel expansion It has been shown to have anticholinergic, hypoglycemic and cholesterol metabolism lowering effects (Lgarashi, K., Sogoigaku, 3, 1 (1951) and Lee Eun-bang, Journal of Pharmacognosy, 5, 49 (1974)). Gil length according to the present invention is used in 5 to 15% by weight, more preferably in 10 to 13% by weight. When it is used below 5% by weight, it is difficult to expect the medicinal effect.When it is used above 15% by weight, it is not supplemented with other herbal ingredients.

Gold and silver coins are also called Indongcho. It has a sweet and cold energy to lower heat and detoxification, to inflame inflammation, to discharge the boil pus. It is widely used for colds, diarrhea, recessive diseases and purulent skin diseases. Gold and silver coins according to the present invention is used in 5 to 15% by weight, more preferably 10 to 13% by weight. When using less than 5% by weight it is difficult to expect the efficacy, and when used more than 15% by weight does not make up with other herbal ingredients.

It is also possible to enhance the taste and aroma by adding the fruit juice or flavoring to the composition of the present invention in a known manner.

Hereinafter, the present invention will be described in detail by the following examples. However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited to the following examples.

Example 1

10g Baekjangjang, 10g of ginseng, 10g of lotus root, 10g of scallop, 10g of apple rock, 10g of tangerine kernel, 10g of bale, 10g of mixed length, pour 200ml of ethanol and heat it at 70 ℃ for 3 hours. After freezing and concentrating for 15 hours at -40 ° C, the resultant was dried in a freeze dryer for at least 35 hours under a pressure of 0.23 Torr or less to obtain 8 g of powder.

Example 2

Proceed in the same manner as in Example 1, except that 100 g of baekgamjang, 10 g of ginseng, 10 g of yeonjak, 10 g of scallions, 10 g of apple rock, 10 g of tangerine kernels, 10 g of bales, 10 g of Gilyeong, 10 g of gold and silver coin were added. 9g of powder was obtained.

Experimental Example

Powders prepared by the above examples were tested for antioxidant activity, hyperlipidemia and diabetes-induced effects and weight loss.

Experimental Example 1

The effects of DPPH on antioxidant activity and LBA lipid peroxidation using TBARS assay were examined.

1) Antioxidant Activity Test by DPPH

According to the contents of FIG. 1, 0.1 mL of a solution prepared in six concentrations of 100, 200, 500, 1000, 2000 and 3000 ppm (99.5% ethanol) by the method of Hatano et al. (Control: 99.5% ethanol) was added 1.9 ml of 0.1 mM DPPH solution (99.5% ethanol). After 10 seconds shaking with the Vortex mixer was incubated for 30 minutes at 37 ℃. Thereafter, the absorbance was measured at 515 nm using an ultraviolet spectrophotometer.

As a positive control drug, L-ascorbic acid was measured by preparing four concentrations of 25, 50, 100, and 200 ppm (99.5% ethanol). The antioxidant activity of each sample was measured by measuring the electron donating ability (EDA%) and IC ₅₀ (μl concentration required to inhibit DPPH radical formation by 50%) for DPPH of Formula 1, and the results are shown in Table 1 and Table 2 below. 3 and 4 are shown.

Sample O.D. : Absorbance of Test Solution Added Example

Control O.D .: Absorbance of Test Solution Added Ethanol Instead of Example

As can be seen in Table 1 and 2, Figures 3 and 4, the activity was shown in the order of Example 2, Example 1 compared with the control drug ascorbic acid, especially Example 2 is the antioxidant activity of DPPH (radical The scavenging activity was excellent. In the case of IC ₅₀ , Example 2 showed an excellent antioxidant capacity of 85.31 ± 0.322 μg / ml and Example 1 of 231.05 ± 26.97 μg / ml.

2) Influence experiment on LDL lipid peroxidation using TBARS method

According to the contents of FIG. 2, in 100 μl of human plasma LDL (Human plasma LDL (400 μg protein)), 16 μl of 1 mM CuSO ₄ , and each sample (25, 50, 100, 200, 500, 1000 and 2000 ppm) prepared for each concentration. PBS (pH 7.4) was mixed to bring the total volume to 1 ml. The mixture was mixed with a Vortex mixer and oxidized by shaking culture for 4 hours in a 37 ° C. water bath, and then 20 µl of 1 mM EDTA was added to stop the oxidation.

1 ml of 25% trichloroacetic acid was added to the oxidized LDL solution to precipitate proteins, and 1 ml of 1% thiobarbituric acid was added to the supernatant, followed by color development at 95 ° C., followed by cooling. The amount of MDA produced was measured using a spectrophotometer at 532 nm.

As an MDA standard sample, 10 nM 1,1,3,3-tetraethoxypropane (1,1,3,3-Tetraethoxypropane) solution was prepared and used.

F: absorbance of standard sample (532 nm)

f: absorbance of the sample (532 nm)

In order to compare the LDL lipid peroxidation inhibitory effect of each sample, the concentration of the sample (IC ₅₀ ) required to inhibit the production of lipid peroxide induced by Cu ²⁺ by 50% was measured and the results are shown in Table 3, Table 4, and FIG. 5 and FIG. 6.

LDL: Unoxidized LDL

Ox-LDL: Cu ²⁺ induced oxidized LDL

[Ox-LDL]: MDA of Cu ²⁺ Induced Oxidized LDL

[Sample LDL]: MDA of LDL oxidized with sample added

[LDL]: MDA of unoxidized LDL

IC ₅₀ : Sample concentration (μg / ml) required for 50% inhibition of Cu ²⁺ induced LDL lipid peroxidation

As can be seen in Tables 3 and 4, FIGS. 5 and 6, the Example 2 administration group, which had a large antioxidant activity compared to the control drug ascorbic acid, exhibited lipid peroxidation inhibitory activity.

Experimental Example 2

Experiments on the effects on hyperlipidemia and diabetes-induced rats

1) Experimental Animal

Sprague-Dawley female rats were purchased from Hallym Farm and were bred at 24 ° C ± 1 ° C for one week and weighed 140 ± 20g in the laboratory.

2) Induced Diabetes

Streptozotocin 50 mg / kg was administered to rats. To measure blood glucose, cholesterol, and triglyceride, blood samples were taken from the inferior vena cava and centrifuged.

3) Blood Sugar, Cholesterol and Triglycerides

Blood samples were centrifuged at room temperature to measure plasma using a conventional spectrophotometer for GOD-glucose analysis, CHOD-PAP cholesterol analysis, and GPO-PAP triglyceride analysis. , 6, 7, FIGS. 7, 8 and 9 are shown. All experimental results calculated the mean and standard error, and the differences between the groups were statistically significant when the p-value was less than 5% using the student t-test.

A) action on blood glucose in the blood

Control: 50 mg / kg streptozotocin (streptozotocin ip.)

Each value represents ± S.E (N = 6).

Significant difference from negative control: ^* p <0.05, ^** p <0.01

As can be seen in Table 5, the control group intraperitoneally administered streptozotocin 50mg / kg blood sugar level of 342.00mg / dl was significantly elevated compared to 115.6mg / dl of normal animals was found to be induced hyperglycemia In the tolbutamide-treated group, a positive control drug for the treatment of diabetes, blood glucose levels were reduced to 117.4 mg / dl.

Next, the hypoglycemic effect of the formulation was remarkable when the Example 2 administration of diabetes mellitus 100 and 200 mg / kg, respectively, and the blood glucose was 155.67 mg / dl, 124.67 mg / dl compared to the control drug It was found to significantly decrease significantly depending on (see FIG. 7).

B) action on total cholesterol in serum

Control: 50 mg / kg streptozotocin (streptozotocin ip)

Each value represents ± S.E (N = 6).

Significant difference from negative control: ^* p <0.05, ^** p <0.01

As can be seen in Table 6, in the control group intraperitoneally administered streptozotocin 50mg / kg cholesterol level 70.0mg / dl slightly increased compared to 58.2mg / dl of normal animals cholesterol concentration when causing diabetes The tolbutamide treatment group, which is a diabetic drug, showed no change to 76.4 mg / dl, indicating that it did not affect blood cholesterol.

On the other hand, when administration of 100, 200mg / kg of Example 2 to the mice that induced diabetes showed cholesterol-lowering activity, it can be seen that the dose-dependent decrease to 67.8, 61.5mg / dl (see Figure 8).

C) action on triglycerides in serum

Control: 50 mg / kg streptozotocin (streptozotocin ip)

50% Methanol Extract

Each value represents ± S.E (N = 6).

Significant difference from negative control: ^* p <0.05, ^** p <0.01

As can be seen in Table 7, in the control group intraperitoneally administered streptozotocin 50mg / kg triglyceride value 123.0mg / dl was significantly increased compared to 60.4mg / dl of normal animals, diabetes In the tolbutamide group, the triglyceride level of triglyceride was significantly reduced to 74.2mg / dl. Meanwhile, when 100 and 200 mg / kg of Example 2 was administered to rats that induced diabetes, triglyceride levels were reduced to 66.3 and 62.3 mg / dl, indicating good activity.

On the other hand, the Example 2 administration group having good antioxidant and lipid peroxidation inhibitory activity showed low activity in triglycerides in diabetic-induced blood, and in particular, as the dose was increased, the triglyceride concentration was increased as well as the concentration of cholesterol (see FIG. 9). ).

Experimental Example 3

Weight loss experiment

1) Experimental Animal

ICR mice were purchased at Hallym Farm, and were bred at 24 ° C. ± 1 ° C. for 1 week and weighed 24 ± 2 g.

2) Experiment Method

Six male ICR mice were weighed before oral administration and administered orally three times a day for three days, and then weighed again three times a day after the last oral dose from day two. Are shown in Table 8 and FIG.

Control: 50 mg / kg streptozotocin (streptozotocin ip)

Each value represents ± S.E (N = 6).

Significant difference from negative control: ^* p <0.05

As can be seen in Table 8, the control group gained weight from 21.8 ± 1.9 g to 23.1 ± 3.0 g, and in the 100 mg / Kg administration group of Example 2 was reduced from 24.2 ± 2.6 g to 23.0 ± 0.5 g, but there was no significant In the 200 mg / Kg administered group of Example 2, it was reduced from 22.7 ± 0.9 g to 20.5 ± 2.7 g, but there was no significance, and in the 400 mg / Kg administered group of Example 2, 28.9 ± 4.9 g and 18.9 ± 4.4 g, respectively. Significant weight loss.

Functional foods effective for anti-diabetic and diet according to the present invention is antioxidant and inhibits absorption by carbohydrates and fats in the small intestine and inhibits absorption, and also absorbs blood glucose, triglyceride and cholesterol Herbal medicine that lowers cholesterol and can be used as food. Antioxidant obesity inhibitor, diet food, and diabetes prevention effect are excellent in combination with white baekjang, ginseng, lotus root, chopsticks, apple rock, tangerine core, packing, gilyeong and gold and silver coin. .

While the present invention has been described in detail with reference to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the scope and spirit of the present invention, and such modifications and variations are included in the appended claims. It is natural to belong.

1 is a schematic diagram showing the antioxidant activity measurement step performed for the embodiments of the present invention.

Figure 2 is a schematic diagram showing the TBARS analysis method of lipid peroxide induced by Cu ²⁺ for the embodiments of the present invention.

Figure 3 is a graph showing the antioxidant capacity measurement by DPPH for the embodiments of the present invention.

4 is a graph showing IC ₅₀ values by DPPH for the embodiments of the present invention.

5 is a graph showing the inhibitory effect of lipid peroxide induced by Cu ²⁺ for the embodiments of the present invention.

FIG. 6 is a graph showing IC ₅₀ values for the inhibition of lipid peroxide induced by Cu ²⁺ for embodiments of the present invention.

Figure 7 is a graph showing the effect on blood glucose when administering the embodiments of the present invention in mice hyperglycemia induced by streptozotocin (streptozotocin).

Figure 8 is a graph showing the effect on blood cholesterol upon administration of the embodiments of the present invention in mice hyperglycemia induced by streptozotocin (streptozotocin).

Figure 9 is a graph showing the effect on the amount of triglycerides in the administration of the embodiments of the present invention in mice hyperglycemia induced by streptozotocin (streptozotocin).

10 is a graph showing the weight loss effect of the groups administered the embodiments of the present invention.

Claims (5)

Baekjeom 5 to 15% by weight, 5-5 to 15% by weight, 5 to 15% by weight of lotus root, 5 to 15% by weight, 5 to 15% by weight of apple rock, 5 to 15% by weight, mandarin kernels 5 to 15% by weight, Dietary composition comprising 5 to 15% by weight and 5 to 15% by weight of gold and silver. 5-15% by weight, 5-15% by weight of ginseng, 5-15% by weight of lotus root, 5-15% by weight of scallop, 5-15% by weight of apple lacquer, 5-15% by weight of tangerine kernels, 5-15% by weight, The composition for anti-diabetic, characterized in that it comprises 5 to 15% by weight and 5 to 15% by weight of gold and silver. 5-15% by weight, 5-15% by weight of ginseng, 5-15% by weight of lotus root, 5-15% by weight of scallop, 5-15% by weight of apple lacquer, 5-15% by weight of tangerine kernels, 5-15% by weight, The composition for hyperlipidemia comprising 5 to 15% by weight and 5 to 15% by weight of gold and silver. 5-15% by weight, 5-15% by weight of ginseng, 5-15% by weight of lotus root, 5-15% by weight of scallop, 5-15% by weight of apple lacquer, 5-15% by weight of tangerine kernels, 5-15% by weight, An antioxidant composition comprising 5 to 15% by weight and 5 to 15% by weight of gold and silver. Functional food, characterized in that it further comprises a fruit juice and flavoring as an additive to the composition of claim 1 to claim 3.