KR20050006592A - Manufacturing method for lycopene bound Zn - Google Patents

Abstract

PURPOSE: A manufacturing method for Zn bound lycopene is provided, which Zn bound lycopene has improved active oxygen-removing activity, so that the Zn bound lycopene can be useful as a raw material for producing health food. CONSTITUTION: The manufacturing method for Zn bound lycopene comprises the steps of: (a) homogenizing the total of lycopene-containing plants such as tomato and watermelon to produce a semi-liquid mass of plants; (b) boiling the semi-liquid mass of plants, adding 50mg to 30g of calcium carbonate per 1kg of the plants, and maintaining the boiling status of the semi-liquid mass of plants to break the cell walls of plants; (c) adding 10 mg to 500 mg of ZnSO4.7H2O per 1kg of plants into the resulting product of step (b) to bind Zn to the lycopene; and (d) heating the resulting product of step (c) to evaporate water, and adding an excessive amount of a solvent containing 95% alcohol and dichloromethane into the evaporated product of step (c) to extract the Zn bound lycopene.

Description

Manufacturing method of lycopene in combination with zinc {Manufacturing method for lycopene bound Zn}

The present invention relates to a method for extracting and purifying lycopene having Zn bound using a plant known to contain lycopene.

Lycopene is a high lipophilic carotenoid that is mainly present in tomatoes and is known to be present in small amounts in other vegetables or algae species, and is always present as a secondary metabolite in mammals, as an antioxidant and as a free radical phagocyte. Plays an important role along with rides, such as vitamin C and other compounds.

Lycopene has also been demonstrated to cooperate with low density lipoproteins that reduce the oxidation of cholesterol and other lipids and thus prevent damage to blood vessels; Furthermore, an inverse correlation between lycopene levels in the blood and prostate cancer has recently been demonstrated. Therefore, the administration of lycopene to the human body plays an important role in reducing the risk of cancer or arteriosclerosis.

In addition, lycopene has been reported to inhibit the growth of HL-60 cells (promyelocytic leukemia cells) and growth inhibition of uterine cancer, breast cancer and lung cancer cells. Tomato-based foods affect the inhibition of prostate cancer. The research is being actively conducted.

In addition, in recent years, the importance of ingestion of trace elements is increasing, the zinc intake as a nutrient is becoming a general trend. Zn is a metalloenzyme, an essential factor that mainly consists of various enzymes and proteins, and is a trace element involved in carbohydrates and fat metabolism. Zinc deficiency causes mildly anorexia and growth retardation. In the case of physiological and clinically obvious zinc deficiency, dermatitis, diarrhea, sore throat, weight loss of liver and spleen, hypogonadism, hepatosplenomegaly, Alkaline Phosphatase (AP) activity, Angiotensin Converting Enzyme (ACE) activity, and zinc in blood Low levels of zinc and hair are known to cause obesity.

In view of the above, the present inventors have completed the method for preparing lycopene, in which Zn is bound as a new functional food, and thus the present invention has been accomplished. According to the present invention, the present invention provides a cancer, arteriosclerosis, Lycopene, a metalloenzyme that has scavenging activity against free radicals that cause diseases such as diabetes mellitus or aging, is an essential factor consisting mainly of various enzymes and proteins. It is to provide a method for producing a combined lycopene Zn that can be used as a raw material for preparing a multi-component health food of Zn and lycopene is coupled to the trace element Zn is also involved.

The present invention to achieve the above technical problem

(a) homogenizing the lycopene-containing plant, such as tomato and watermelon, into a semi-liquid mass;

(b) adding 50 mg to 30 g of calcium carbonate per 1 kg of plant weight after heating the resultant of step (a) until boiling, and maintaining the boiling state to a certain degree to destroy the plant cell wall;

(c) adding 10 mg to 500 mg of ZnSO ₄ 7H ₂ O per kg of plant weight to the result of step (b) to bind Zn to lycopene; And

(d) continuously heating the resultant of step (c) and evaporating water, followed by adding an excess solvent in which 95% alcohol and dichloromethane are mixed to extract Zn-bound lycopene. Provided is a method for preparing lycopene with Zn.

In the method for preparing Zn-bound lycopene according to the present invention as described above, after removing the solvent from the extract of step (d) to obtain crystals of Zn-bound lycopene, after dissolving it again, impurities are formed using a resin tower. It may further comprise a purification step to remove.

The method for preparing Zn-coupled lycopene according to the invention as described above may further include the step of drying or lyophilizing the purified extract using a dryer to obtain lycopene Zn-coupled in a powder state.

Hereinafter, a method for preparing lycopene Zn-coupled according to the present invention will be described in detail.

First, plants known to contain lycopene, such as tomatoes and watermelons, are homogenized with a mixer or knife mill to obtain a watery, semi-liquid mass of water.

The semi-liquid lump is placed in a container and heated to a boil with moisture inside, and then calcium carbonate is added. The reason for adding the calcium carbonate is to break the cell wall so that the lycopene existing in the cell is leaked to the outside, and the reason for heating to boiling is to promote the destruction of the cell wall.

The added amount of calcium carbonate is not particularly limited, but if the amount of calcium carbonate is too large, alkalinity becomes strong, which is a problem when used as a food, and as a result of checking through various tests of the present inventors, adding 50 mg to 30 g of calcium carbonate per 1 kg of plant weight It is proved to be the most appropriate, and other alkaline chemicals can be used to break the cell wall, but calcium carbonate, which is weakly alkaline, is considered to be the most suitable since it is intended to produce raw materials for use as a health food.

In addition, after adding calcium carbonate, it is preferable to maintain a boiling state for a certain time in order to destroy cell walls of sufficient plants.

Next, while stopping the heating or continuing heating the additional ZnSO ₄ 7H ₂ O reason for adding .ZnSO ₄ 7H ₂ O is to couple the Zn lycopene in the cell walls are destroyed cells flow out. In this case, Zn may be bound to other intracellular components in addition to lycopene, but in the subsequent extraction step, Zn is bound to lycopene.

The amount of ZnSO ₄ 7H ₂ O is not particularly limited, but it is preferable to add 10 mg to 500 mg of ZnSO ₄ 7H ₂ O per kg of plant weight.

Next, the semi-liquid mass in the vessel is continuously heated to reduce the moisture and concentrated to perform the extraction step for extracting the lycopene bound Zn.

When it is concentrated to some extent, the heating is stopped, and after cooling to room temperature, an excess of a solvent mixed with 95% alcohol and dichloromethane is added, and the mixture is left for a certain time to extract Zn-bound lycopene.

Through the process as described above, the extraction of the lycopene Zn-coupled according to the present invention is completed, but there is a need to proceed further purification process. The purification process is as follows.

First, the solvent is evaporated from the lycopene extract having the Zn-bound bond to precipitate the lycopene extract to obtain crystals. Subsequently, after dissolving the crystal in a 95% alcohol alcohol and dichloromethane mixed solvent, Zn-bound lycopene may be purified by passing an ion exchange resin column to remove impurities in the solution.

In order to practically use the lycopene combined with purified Zn after extraction or extraction, the method described above needs to be prepared in powder form. The lycopene combined with purified Zn after extraction or extraction is dried in a dryer or rapidly frozen. The cake obtained by lyophilization, which is then dried at or below the triple point, may be prepared by pulverizing the cake.

As described above, according to the method of the present invention, lycopene having a scavenging activity against active oxygen known as one of the substances causing cancer, arteriosclerosis, diabetes, or aging promoting agent is mainly referred to as metalloenzyme. It is an essential factor constituting various enzymes and proteins, and Zn, which is a trace element that is also involved in carbohydrates and fat metabolism, is combined, and Zn is combined with Zn, which can be used as a raw material for the preparation of Zn and lycopene It can manufacture.

Hereinafter, a method for preparing lycopene Zn-coupled according to the present invention will be described in detail with reference to Examples.

<Example 1>

After washing 1 kg of tomatoes cleanly, the whole was ground using a knife mill without removing the skin to obtain a semi-liquid mass in which a liquid component and a mass component were mixed.

This semi-liquid mass was then transferred to a vessel, poured and heated until boiling. After boiling 300 mg of calcium carbonate was added at the start of boiling, the boiling state was maintained for 30 minutes.

Next, while keeping boiling, ZnSO ₄ 7H ₂ O 200mg was added and continued heating to visually check, and concentrated until the capacity of the semi-liquid mass in the container was reduced by half.

After the concentration was completed, the heating was stopped, and after cooling to room temperature, 2 liters of a solvent in which 95% alcohol alcohol and dichloromethane were mixed in the same amount was added and mixed.

After the mixing of the extraction solvent was completed, the extraction solvent was left until it was separated from the semi-liquid mass, and then separated. After the recovery solvent was recovered, the extraction solvent was evaporated to obtain Zn-bound lycopene crystals.

Subsequently, Zn-bonded lycopene crystals were dissolved again in a solvent in which 95% alcohol alcohol and dichloromethane were mixed in the same amount to make a solution, and then passed through an ion exchange resin tower to remove impurities. Drying and grinding to prepare a lycopene Zn-bonded in a powder form.

As a result of confirming the powder prepared by HPLC, electron microscopy, etc., it was confirmed that the lycopene has a structure in which Zn is bound.

<Example 2>

After washing 10 kg of watermelon cleanly, the whole was ground using a knife mill without removing the skin to obtain a semi-liquid mass in which a liquid component and a mass component were mixed.

This semi-liquid mass was then transferred to a vessel, poured and heated until boiling. The boiling state was maintained for 30 minutes after adding 3000 mg of calcium carbonate at the time of boiling.

Next, 2000 mg of ZnSO ₄ 7H ₂ O was added while maintaining a boiling state and continuously heated to visually check, and the concentration of the semi-liquid mass in the container was reduced to half.

After the concentration was completed, the heating was stopped, and after cooling to room temperature, 5 liters of a solvent in which 95% alcohol alcohol and dichloromethane were mixed in the same amount was added and mixed.

After the mixing of the extraction solvent was completed, the extraction solvent was left until it was separated from the semi-liquid mass, and then separated. After the recovery solvent was recovered, the extraction solvent was evaporated to obtain Zn-bound lycopene crystals.

Subsequently, Zn-bonded lycopene crystals were dissolved again in a solvent in which 95% alcohol alcohol and dichloromethane were mixed in the same amount to make a solution, and then passed through an ion exchange resin tower to remove impurities. Drying and grinding to prepare a lycopene Zn-bonded in a powder form.

As a result of confirming the prepared powder through HPLC, electron microscopy, etc., it was confirmed that Zn is bound to lycopene.

As described above, according to the present invention, lycopene having a scavenging activity against free radicals that cause diseases such as cancer, arteriosclerosis, diabetes, or one of the aging-promoting agents, are mainly various as metalloenzymes. It is an essential factor constituting enzymes and proteins, and Zn, a trace element that is also involved in carbohydrates and fat metabolism, is combined to produce lycopene combined with Zn, which can be used as a raw material for the preparation of Zn and lycopene. Can be.

Claims (3)

(a) homogenizing the entire plant containing lycopene, such as tomato and watermelon, into a semi-liquid mass; (b) adding 50 mg to 30 g of calcium carbonate per 1 kg of plant weight after heating the resultant of step (a) until boiling, and maintaining the boiling state to a certain degree to destroy the plant cell wall; (c) adding 10 mg to 500 mg of ZnSO ₄ 7H ₂ O per kg of plant weight to the result of step (b) to bind Zn to lycopene; And (d) continuously heating the resultant of step (c) and evaporating water, followed by adding an excess solvent in which 95% alcohol and dichloromethane are mixed to extract Zn-bound lycopene. Lycopene manufacturing method combined with Zn. The method of claim 1, Zn-coupled, characterized in that it further comprises a purifying step of removing impurities using a resin tower after removing the solvent from the extract of step (d) to obtain crystals of Zn-bound lycopene and re-dissolving it again. Lycopene production method. The method according to claim 1 or 2, Zn-coupled lycopene manufacturing method characterized in that to obtain a lycopene Zn-bonded in a powder state by drying or lyophilized purified extract using a dryer.