KR20040106102A - The method enhancing quality of bean sprouts using the composition comprising of the copper sulfate - Google Patents

Abstract

PURPOSE: Provided is a method for increasing the quality of bean sprouts by using a preparation comprising copper sulfate. The preparation improves the color, side and th like of bean sprouts and inhibits disease occurrence therein. CONSTITUTION: The method for increasing quality of bean sprouts is characterized by injecting a preparation comprising copper sulfate while cultivating bean sprouts, wherein the copper sulfate is dissolved in water and used in the concentration of within 0.01ppm to 1000ppm. Therefore, it improves the color, size and the like of bean sprouts and inhibits disease occurrence therein.

Description

The method enhancing quality of bean sprouts using the composition comprising of the copper sulfate}

The present invention relates to a method for improving the quality of bean sprouts using a formulation consisting of copper sulfate.

Sprouts are nutritionally high in vitamin A, vitamin C and fiber, and can be grown for a short time regardless of place and season. . In recent years, the Doochae Association has been contracting soybean sprouts with local agricultural cooperatives for the supply and supply of domestic quality soybean sprouts.

However, researches on improving the quality of soybean sprouts have only been carried out on how to increase the production by treating growth regulators, and the results of the research on quality improvement are very poor.

The quality of bean sprouts can be thought of in two ways.

First is the apparent quality of the bean sprouts. When consumers choose bean sprouts, they usually look and judge the apparent quality of the bean sprouts. The apparent quality of bean sprouts depends not only on the size of the bean sprouts, but also on the thickness and thickness of the hypocotyl, as well as the length of the roots and the presence of fine roots.The bean sprouts are clear and glossy, the proportion of roots are small and the roots are not generated. It is evaluated as excellent. In particular, the cotyledon part has vivid yellow yellow color and silver white color from stem to root tip is very popular among consumers.

Therefore, the development of the bean sprouts with excellent appearance as described above is necessary, but the mechanism of expressing the exact expression mechanism and excellent color of the bean sprout color so far is little known state.

Second, the quality of soybean sprouts depends largely on the degree of decay that occurs during the growing process. The bean sprout cultivation environment has high humidity due to frequent irrigation, and the temperature rises due to dark conditions and respiration heat during the cultivation process, and it is difficult to ventilate. Therefore, as the environment suitable for the growth and propagation of the microorganisms inherent in the bean sprout seed beans is made, it causes rot of the germinating seed bean or the cultivating bean sprouts. Currently, about 30% of the total cultivation of soybean sprouts is caused by soybean sprout rot, which does not become germinated or germinate, which causes enormous damage to bean sprout growers.

Bean sprout rot is divided into fungal rot and bacterial rot.

Fungal decay is mainly caused by fungi in the fungus, representative fungi are Fusarium moniliform (ATCC 10052), Fusarium oxysporum (ATCC 60841) and Fusarium solani , ATCC 11712) strain, and the like.

Fungus in Fussium causes a significant outbreak around 6-7 days immediately before harvesting soybean sprouts, causing damage to the middle and lower portions of the bean sprouts' hypocotyls, significantly reducing commodity value. About 3-5 days after seed germination, a light brown external symptom appears in the middle and lower parts of the bean sprouts, and about 6-7 days after germination, the symptoms progress further and dark brown external symptoms develop and decay progresses. do.

Bacterial rot is caused by Erwinia carotovora subsp. Carotovora (ATCC 15390) or Pseudomonas putida biovar A strains.

Erwina carotoora subspecies strain is a representative strain that causes soybean sprout purifying disease, and the seed discoloration, germination is inhibited, and bite is decayed from 2-3 days after cultivation. The cotyledon region also has the same discoloration as the seeds, and the symptom of restraining the kidney of the hypocotyl is characterized.

Pseudomonas putida biobai strains begin to develop about 5 days after cultivation, and rapidly spread to the entire bean sprout in the growing container after 1 to 2 days. In the early stages of symptom, light brownish, immersive lesions appear in the abdominal axis, and the entire abdominal axis becomes translucent light brown, causing decay, which is known to be caused by the accumulation of toxic substances secreted by these bacteria. .

Therefore, the rot phenomenon occurring during the cultivation of bean sprouts can be summarized as rot caused by Erwinia cartoboro subspecies at the beginning of cultivation and by fungi and Pseudomonas putida biovail strains in Fujian in the middle and late cultivation period.

Until now, the method developed to prevent soybean sprouts has been attempted to suppress the onset of microorganisms causing soybean sprout rot by irrigation by lowering the acidity of water used for cultivating bean sprouts to pH 5 or less. The lowering method was not effective because it was uneconomical and not suitable for growers who did not want to change the method of growing bean sprouts.

Therefore, in order to develop quality through improving the color and health of the bean sprouts, the situation for a simple but remarkably high effect is required.

In order to achieve the above object, the present invention is to prepare a formulation consisting of copper sulfate, it is used in the cultivation of bean sprouts to provide a method that can improve the color and health of the bean sprouts.

The present invention relates to a method for improving the quality of bean sprouts using a formulation consisting of copper sulfate.

The copper sulfate formulation of the present invention is a formulation dissolved using a solvent capable of dissolving copper sulfate, and the copper sulfate formulation of the present invention is dissolved using water as a solvent.

In the copper sulfate formulation of the present invention, the concentration of copper sulfate is preferably used in the range of 0.1 ppm to 1,000 ppm.

The copper sulfate formulation of the present invention may be added with components known to exhibit an excellent effect on improving the quality of the bean sprouts as necessary.

When the copper sulfate preparation of the present invention is used for bean sprouts, one of the methods of immersing seed beans in the copper sulfate preparation of the present invention, spraying the copper sulfate preparation of the present invention on seed beans, or irrigation of the copper sulfate preparation of the present invention when cultivating bean sprouts is selected. Can be used.

When the copper sulfate preparation of the present invention is used for cultivation of bean sprouts, the color development of bean sprouts can be greatly improved, and the occurrence of soybean sprouts and rot diseases of bean sprouts can be significantly suppressed.

Therefore, since the copper sulfate formulation of the present invention can obtain high quality bean sprouts through a simple manufacturing method and use method, it is possible to increase the economical and commercial properties in bean sprouts cultivation, useful for bean sprout growers and general households who want to produce bean sprouts of high quality. Can be used.

Copper sulfate formulation of the present invention will be described in more detail in the following Examples, but the present invention is not limited by the Examples.

Example 1 Checking the Effect of Copper Sulfate Formulation of the Present Invention on Color Selection of Bean Sprouts

In order to confirm the effect of the copper sulfate formulation of the present invention on the color of bean sprouts, the experiment was carried out as follows.

In order to prepare the copper sulfate preparation of the present invention, after dissolving copper sulfate completely in water, the final concentration of copper sulfate is 0.01 ppm, 0.1 ppm, 1 ppm, 10 ppm, 100 ppm, 1,000 ppm to the preparation containing copper sulfate at various concentrations Were prepared.

Bean sprout seed beans were prepared by purchasing domestic and Chinese beans from a commercial bean sprout seed bean distributor.The two domestic seeds were seed bean # 1 and seed bean # 2 respectively, and the two Chinese seeds were seed bean # 3 and seed bean # 4 respectively. Named it.

Each, 100g of seed beans were prepared, and the control group was immersed in tap water for 4 hours, and the experimental group was immersed in various concentrations of copper sulfate solution prepared in Example for 4 hours. After soaking, the seed beans of the control group and the experimental group were placed in plastic containers with holes at the bottom, and were cultivated for 10 days while being irrigated with tap water six times at intervals of four hours a day under dark conditions.

After 10 days of harvesting, the color selection of each bean sprout was investigated. In order to increase the objectivity of the investigation, people who had no knowledge of the experiment were evaluated for color selection, and the results are shown in Table 1.

TABLE 1

(+: Poor color, ++: normal color, +++: slightly better color, ++++: excellent color, +++++: very good color)

As shown in Table 1, the color scent was found to be very good in the group treated with the copper sulfate formulation of the present invention. That is, in the group treated with copper sulfate, the cotyledon region was vivid yellowish yellow and silvery white from the stem to the root.

Therefore, it can be seen that the copper sulfate formulation of the present invention has a very excellent effect on the color tack improvement of bean sprouts.

[Example 2] Confirmation of the effect of copper sulfate formulation of the present invention on soybean sprouts and rot diseases

In order to confirm the effect of the copper sulfate formulation of the present invention on the soybean sprouts and rot diseases of cultivated bean sprouts, the experiment was carried out as follows.

1) Effect on Soybean Sprout Decay

First, an experiment for determining the effect of the copper sulfate formulation of the present invention on the microorganisms involved in the decay of bean sprouts was performed using the copper sulfate formulations prepared in Example 1 as follows.

The microorganisms involved in the decay of bean sprouts are Fusarium moniliform (ATCC 10052), Fusarium oxysporum (ATCC 60841), Fusarium solani (ATCC 11712), Erwinia carotovora subsp. Carotovora (ATCC 15390) was prepared by obtaining Pseudomonas putida biovar A strains from Korea University, respectively.

Each strain was mixed with various concentrations of copper sulfate preparations prepared in the same manner as in Example 1, then cultured in a medium, and the sterilization effect of copper sulfate on each strain was observed, and the results are shown in Table 2.

As shown in Table 2, the copper sulfate formulation of the present invention exhibited a poor bactericidal effect of 40-60% against the bean sprout rot strains.

TABLE 2

Therefore, it can be seen that the copper sulfate formulation of the present invention has a direct bactericidal effect against soybean sprout rot strains.

2) Effect of Copper Sulfate Formulation of the Present Invention on the Development of Soybean Disease in Soybean Sprouts

When the copper sulfate formulation of the present invention is used in cultivation of bean sprouts, it was confirmed as follows whether or not the occurrence of bean sprouts beetles can be actually suppressed.

Bean sprout seed beans were prepared by purchasing domestic and Chinese beans from a commercial bean sprout seed bean distributor.The two domestic seeds were seed bean 1 and seed bean 2, respectively, and the two Chinese seeds were seed bean 3 and seed bean 4, respectively. Named.

100 g of each seed bean was prepared, and the control group was immersed in tap water for 4 hours, and the experimental group was added to the copper sulfate solution of various concentrations prepared in Example 1 for 4 hours. After soaking, the seed beans of each of the control group and the experimental group were placed in plastic containers with holes at the bottom, and cultivated for 10 days while irrigated with tap water six times at intervals of 4 hours a day under dark conditions.

The incidence of purulent disease caused by the Erwina cardovara bacterial strain was examined from the second day after hemorrhage of seed beans. After 20 repetitions to reduce the error of the experiment, the results are shown in Table 3.

TABLE 3

As shown in Table 3, the copper sulfate formulation of the present invention significantly reduced the incidence of soybean sprouts of sprouts, and the disease was completely suppressed when the concentration of copper sulfate was 10 ppm or more.

3) Effect of Copper Sulfate Formulation of the Present Invention on the Actual Development of Corruption Disease during Soybean Sprout Cultivation

When the copper sulfate preparation of the present invention is used for cultivation of bean sprouts, it was confirmed in the same manner as in Example 2, 2) that the occurrence of sprouts sprouts can be suppressed.

The rot caused by the fungus fungus and Pseudomonas putida biovail strain was investigated from 6 days after hemorrhoids. After repeated 20 times to reduce the error of the experiment, the results are shown in Table 4.

TABLE 4

As shown in Table 4, the copper sulfate formulation of the present invention significantly reduced the incidence of rot of soybean sprouts, and the disease was completely suppressed when the concentration of copper sulfate was 1 ppm or more.

Therefore, the copper sulfate formulation of the present invention exhibits low bactericidal power against the causative agents of soybean sprouts and rot, but it can be seen that the treatment of soybean sprout seed beans can significantly suppress the occurrence of rot and rot.

The copper sulfate formulation of the present invention exhibits an effect of greatly improving color appearance of bean sprouts.

In addition, the copper sulfate formulation of the present invention has an effect of remarkably suppressing the bruises and rot of soybean sprout seed beans.

Therefore, if the cultivation using the copper sulfate formulation of the present invention can obtain a good quality bean sprouts by a simple cultivation method, it can be expected to obtain the effect of obtaining high economical efficiency and commercial properties in bean sprouts cultivation.

Claims (6)

How to improve the quality of bean sprouts by injecting copper sulfate during cultivation The method for improving the quality of bean sprouts according to claim 1, characterized by copper sulfate dissolved using water as a solvent. The method for improving the quality of bean sprouts according to claim 2, wherein the concentration of copper sulfate is used in the range of 0.01 ppm-1,000 ppm. The method for improving the quality of bean sprouts according to any one of claims 1 to 3, which improves the color selection of the bean sprouts. The method for improving the quality of bean sprouts according to any one of claims 1 to 3, wherein the sprouting of the bean sprouts is suppressed. The method for improving the quality of bean sprouts according to any one of claims 1 to 3, wherein the sprouting of the bean sprouts is suppressed.