KR20040102766A - Cosmetic composition containing niosome with tranexamic acid and supercritical fluid extract of phellinus linteus - Google Patents

Abstract

PURPOSE: Provided is a cosmetic composition containing niosome including tranexamic acid and the supercritical fluid extract of Phellinus linteus. Therefore, the composition increase collagen synthesis and skin elasticity to prevent skin wrinkles, and reduces melanogenesis to whiten the skin. CONSTITUTION: The cosmetic composition is characterized by containing 0.001-15 wt.% of niosome which includes 0.01-20.0 wt.% of the mixture of tranexamic acid and the supercritical fluid extract of Phellinus linteus in a mixing ratio of 1:0.1-10.0.

Description

COSMETIC COMPOSITION CONTAINING NIOSOME WITH TRANEXAMIC ACID AND SUPERCRITICAL FLUID EXTRACT OF PHELLINUS LINTEUS}

The present invention relates to a cosmetic composition containing a tranexamic acid and a super mushroom extract, and more particularly, to a cosmetic composition containing a stabilized niexome and a supercritical extract.

The skin of modern man is exposed to various harmful environment and stresses and suffers various damages. As he ages, the physiological activity of the skin decreases and the recovery rate of damaged skin is slowed down, and the dryness, decrease of elasticity, wrinkle formation, etc. Will meet various skin aging phenomena. In addition, the irradiation of ultraviolet rays from sunlight generates spots, freckles and the like due to pigmentation in the skin. On the other hand, various cosmetic materials for improving skin aging, pigmentation, etc. have been developed.

Liposomes are a special kind of liposomes that are used in drug delivery systems, and are widely used not only in the pharmaceutical field but also in cosmetics and food. This has the advantage that the bioactive ingredient can be stably delivered in an unbroken state, and that the bioactive ingredient can be contained in both fat-soluble and water-soluble.

Liposomes are largely divided into MLV (Multilamella vesicle) and monolithic liposomes (ULV: Unilamella vesicle), and ULV can be divided into Large Unilamella vesicle and Small Uni lame lla vesicle. MLV is 400-3500 nm in size and 5-15% of the amount can be contained in liposomes. LUV has a size of 100-1,000 nm, 36-65% of the amount can be contained in liposomes, and SLV has a size of 20-50 nm, and 0.5-1.0% of the amount can be contained in liposomes. The amount of bioactive substances that can be contained is the most LUV but unstable, SLV is the most stable but the amount that can contain less. In addition, the structure of the human skin has a multi-layered structure, so MLV has affinity with the skin, but its size is so large that it is difficult to penetrate the skin.

Niosome, on the other hand, has a size of 10-50 nm, and in its constituents, unlike liposomes consisting of phospholipids, ceramide, cerebromide, glycolipid, sphingomyelin Sphingolipid derivatives, such as), are used to form vehicles and to incorporate bioactive components in the vehicle.

Phellinus linteus belongs to the Phellinus Quel. Em. Lmaz of the Hymenochaetaceae family and refers to the woody mud mushroom (Phellinus Linteus) in Korea. In this mud mushroom, about 48 kinds of natural situation mushrooms are reported around the world, and in Korea, rusty mud mushrooms (P.ferruginosus), dried mud mushrooms (P. gilvus), horse mud mushrooms (P. igniarius), and branch mud mushrooms ( There are eight kinds of natural situation mushrooms including P. laevigatus, P. pomaceus, P. robustus, fir mud and P. pini.

These situational mushrooms have pharmacological effects such as anticancer effect, immune enhancing function and tumor suppression effect, and are known to be effective in the treatment of gynecological diseases such as uterine bleeding and menstrual irregularities (Immune activity of woody mud (situation) mushroom) Vol.26 (1) pp.86-90, 1998 Song Chi-Hyun.In vitro and in vivo Anticancer Activity of Situation Mushroom Fruiting Body Korean Journal of food science and technology Vol.32 (2) pp.477-480, 2000 A study on the immunomodulatory function of mycelium Korean journal of mycology Vol.24 (2) p.142-148, 1996 Bae Bae Jong).

In addition, the situation mushroom extract contains a large amount of various kinds of sugars, flavonoids, fiber, isoflavones, etc. When used as a cosmetic, it is excellent in moisturizing, anti-aging and anti-oxidant effects, especially in terms of skin whitening effect. Changes in Polysaccharide Production and Polysaccharide Composition of Situation Mushrooms According to Culture Conditions Journal of mycology Vol.23 (4) pp.325-331, 1995 Postdoctoral Training Course Vol.40 pp.51-56, 1998

In this regard, in recent years, products containing natural situation mushroom extracts having a whitening effect and an antioxidant effect have been developed in the pharmaceutical and cosmetic fields, but products having more excellent effects are required.

On the other hand, for tranexamic acid, it was reported that the intraoral wound effect (Journal of Korean Asspcoiation of Oral and Maxillifacual Surgeons 1987198713 1 217217 ~ P233 Lee Young-seok), antimicrobial effect on periodontal pathogens and bad breath-causing agents (Journal of the Korean) Academy of Dental Health 1998 22 3 P205 ~ P214 Moon Hyuk-su), The Journal of Korean Society of Anesthesiologist 1996 31 5 P634 ~ P639 Shinchiman, Restoration of skin barrier function (J Invest Dermatol. 1997 Jul; 109 (1): 84-90.Denda M), skin wound healing enhancing effect (Int J Oral Maxillofac Surg. 1988 Aug; 17 (4): 275-6.Bjorlin G, Nilsson IM.) A Comparative Study of Bleeding in Goat Treated with Anticoagulant (Journal of Korean Asspcoiation of Oral and Maxillifacual Surgeons, 1987, # 13, # 1, P207, P216, Se., Invest Clin. 1998 Jun; 39 (2): 77- 83.Spanish Bernardoni-Socorro C) has been studied. It was. In the patent document related to Tranexamsan, a pre-formed gel sheet (Domestic Application Publication No. 1020027000213), a sheet-like device (Domestic Application Publication No. 1020027000208), a transdermal absorbent using polymer nanoparticles, and an external preparation composition containing the same (Domestic Application Publication) No. 1020010019869), cleaning products for skin and hair in which skin protection active ingredients are deposited (National Application Publication No. 1020007011848), preparation method of sustained-release microspheres by the multiple emulsion method (Domestic Application Publication No. 1020000036178), traneck Samsan derivatives and skin external preparations containing them (Domestic Application Publication No. 1019947000952).

Recently, due to the rise of environmental problems, a new extraction method is being developed to replace the existing extraction method using organic materials. Among these methods, a method of applying supercritical fluid technology to natural product extraction has been tried as an extraction method applicable to the human body by minimizing the toxicity of residual materials as much as possible. Supercritical fluids generally exist above critical temperatures and pressures, and have a low viscosity and high mass transfer ability due to the intermediate nature of gases and liquids. Representative supercritical fluids include carbon dioxide, which are widely used because they have advantages such as nontoxic environmental friendliness at low critical temperatures and pressures (supercritical extraction (SFE) and solvent extraction of β-ecdysone in the dew) , Journal of the Korean Society of Agricultural Chemistry and Biotechmology Vol. 44 (3) pp.197-201, 2001, Shim Jae-Hwan, Soil Residue Analysis of Quinclorac in HPLC by Supercritical Extraction and Fluorescent Inductor, Journal of the Korean Agricultural chemistry and biotechnology Vol.40 (5) pp.442-446, 1997, Yong-hwan Kim, Extraction of Phenol Compounds from Ginkgo Biloba Leaves by Supercritical Extraction, Journal of Pharmacognosy Vol.25 No.1 [1994] 85-86, Hur Hoon) .

Applicants have tried to mix various substances based on the situation mushroom supercritical fluid extract from which more effective physiologically active ingredients were extracted through supercritical fluid extraction in order to obtain a cosmetic having an effective anti-wrinkle and whitening effect. By blending samsan and stabilizing with niosomes, it was found that a synergistic effect beyond the additive effect of the active ingredient was obtained and completed the present invention.

Therefore, an object of the present invention is to obtain a cosmetic containing tranexamic acid and super mushrooms supercritical extract, which is excellent in anti-wrinkle, whitening effect and maximized on the skin.

In order to achieve the above object, according to the present invention, characterized in that it contains 0.01 ~ 20.0% by weight relative to the total weight of the composition of the tranexamic acid and mushroom mushroom supercritical extract, containing the tranexamic acid and mushroom mushroom supercritical A cosmetic composition containing niosomes is provided.

Hereinafter, the present invention will be described in detail.

As a situation mushroom of this invention, all the situation mushroom which belongs to the mud mushroom genus with pine-scale mushroom can be used. It is preferable to use wild mushrooms, and it is particularly preferable to use wild mushrooms native to Korea.

Supercritical extract is prepared from these situational mushrooms.

In order to prepare a supercritical extract, first the dried mushrooms are dried and then ground to prepare a mushroom mushroom powder.

As the extraction solvent, polyhydric alcohols such as isopropyl alcohol, propylene glycol, butylene glycol, and the like can be used, and 1,3-butylene glycol and ethanol are mixed in the same mass ratio, followed by stirring to form a single phase and co-solvent. It is preferable to use as.

Supercritical fluid extraction used in the present invention can be carried out by a conventional method using carbon dioxide, specifically, it is preferably carried out as follows.

The ground mushroom and cosolvent are mixed and stirred. The mixing ratio is the mass ratio of the pulverized mushroom and the cosolvent is 1: 2.5 ~ 6.5, preferably using a mass ratio of 1: 4 is the best in terms of yield. Subsequently, the mixture is introduced into a supercritical extraction tank, and carbon dioxide is supplied to the extraction tank using a high pressure gas pump to increase the pressure. Then, the temperature inside the extraction tank is raised to 40 ° C. and maintained therein. Then, while continuously supplying carbon dioxide to the extraction tank, the extract is released using a pressure regulator attached to the outlet side. By controlling the temperature of the pressure regulator, the pressure and temperature drop of the discharge prevent carbon dioxide from transferring to solid dry ice and clogging the tube at the final outlet. As for this temperature, about 60 degreeC is preferable. The released extract is decompressed to atmospheric pressure to separate carbon dioxide and mushroom extract, carbon dioxide is released into the atmosphere and the mushroom extract is recovered. The process may be repeated several times to increase the yield of the extract.

Tranexamic acid can be used as it is, or can be used, melt | dissolving in solvents, such as 1, 3- butylene glycol and ethanol, or a mixed solvent thereof. Especially, it is preferable to use the same amount liquid mixture of ethanol and 1, 3- butylene glycol.

Subsequently, the mushroom extract and tranesamic acid are mixed. The mixing ratio is preferably 1: 0.1 to 10.0, more preferably 1: 0.5 to 1.5, and most preferably 1: 1. In the case of using tranexamic acid as a solution, the ratio of tranexamic acid in the tranexamic acid solution is as described above.

As niosomes for stabilizing the tranexamic acid and the situation mushroom supercritical extract, it is easy to penetrate the skin, can contain excessive water-soluble components and the size of 10 ~ 50nm, niosome prepared to have a closed double layer structure to use Can be.

In order to contain the tranexamic acid and the situation mushroom supercritical extract in the niosomes, the components of the niosomes are heated and uniformly mixed and dissolved to obtain a niobium, wherein the extract of the tranexamic acid and the situation mushroom supercritical Add the mixture and pass the mixture through a Microfludizer.

Through the above process, by effectively stabilizing into the closed bilayer structure of niosome, the active ingredients of the tranexamic acid and situation mushroom supercritical extract, the effect of the tranexamic acid and situation mushroom supercritical extract can be maximized.

Tranexamic acid and super mushroom mushroom supercritical extract is preferably included in an amount of 0.001 to 15% by weight, particularly preferably 1 to 15% by weight, based on the total weight of niosomes containing the same, including 5.0% by weight. Most preferably. When the content is less than 0.001% by weight, the effect is insignificant, and when the content is more than 15% by weight, the synergy is not sufficient. At this time, when using the tranexamic acid in a solution state, a mixture of the tranexamic acid and the situation mushroom supercritical extract contained in the tranexamic acid solution is included in the above content for niosomes.

Prepared as described above, niosome containing tranexamic acid and superficial mushroom supercritical extract according to the present invention, skin, such as lotion, nourishing longevity, nutrition cream, essence, pack, cleansing cream, cleansing foam, cleansing water, massage cream It can be added to the elasticity, wrinkle improvement and whitening-related cosmetics, it is preferred to add 0.01 to 20.0% by weight based on the total weight of the composition. If it is less than 0.01% by weight, the efficacy is too small, and if it is more than 20.0% by weight, the feeling of use as a cosmetic is poor, which is not suitable for use in thin products such as lotions.

The following Examples, Comparative Examples, Preparation Examples, and Test Examples are provided to explain the present invention in more detail, and the scope of the present invention is not limited thereto.

(Example)

(Preparation of natural situation mushroom supercritical extract)

As a natural mushroom, P. linteus and P. pini, native to the Korean Peninsula, are dried in a cool place without sunlight and mixed in the same amount. It was ground to a size of 1 cm in diameter or less by a mechanical method. In addition, 1,3-butylene glycol and ethanol were mixed in the same mass ratio and stirred to prepare a single-phase cosolvent.

The mixed mushroom and cosolvent were mixed at a mass ratio of 1: 4 and stirred for 30 minutes, and then the mushroom and cosolvent mixture was put into a supercritical extraction tank having an internal volume of 1.5 L and the extraction tank was sealed. After supplying carbon dioxide to the extraction tank and increasing the pressure by using a high-pressure gas pump, the supply of carbon dioxide was stopped when the pressure of the extraction tank reached 300 atm.

The temperature inside the extraction tank was raised to 40 ° C. at a rate of 0.5 / min using a heating wire wrapped outside the extraction tank using a PID temperature controller. The pressure of the extraction tank due to the elevated temperature was partially discharged by the pressure regulator attached to the outlet of the extraction tank and maintained at 300 atm. In addition, the temperature and pressure of the extraction tank were kept at 40 ° C and 300 atmospheres for 30 minutes.

The high pressure gas pump was restarted and carbon dioxide was continuously supplied to the extraction tank at a flow rate of 12 l / min. At the same time, the extract was released using a pressure regulator attached to the outlet, while maintaining the temperature of the pressure regulator at 60 ° C. The released extract was decompressed to atmospheric pressure to separate carbon dioxide and mushroom extract and carbon dioxide was released into the atmosphere. The separated mushroom extracts were recovered using a glass collector. During the above process, the process of recovering the separated mushroom extract from the step of raising the temperature inside the extraction tank to 40 ° C. at a rate of 0.5 ° C./min was repeated several times for 10 hours, and then the extraction was terminated. It was used as a sample (hereinafter referred to as sample S1).

(Preparation of Tranexamic Acid Solution)

The same mixture of ethanol and 1.3-butylene glycol and tranexamic acid were mixed at a weight ratio of 1: 1, and stirred at room temperature for 2 hours to dissolve and used as a sample (hereinafter referred to as sample S2).

(Preparation of a mixture of Tranexamic acid solution and supercritical mushroom supercritical extract)

The supernatant mushroom supercritical extract (S1) and the tranexamic acid solution (S2) were mixed, but the weight ratio of the supernatant mushroom supercritical extract and the tranexamic acid in the tranexamic acid solution was mixed to be 1: 1. It was stirred for 2 hours at room temperature to dissolve and used as a sample (hereinafter referred to as sample S3).

(Preparation of Niosome Containing Natural Situation Mushroom Supercritical Extract)

The phase A composition of Table 1 was heated to 95 ° C., mixed and dissolved uniformly to obtain niobium, which was cooled to 40 ° C., followed by mixing with phase B. A mixture of A and B was passed through a high pressure emulsifier three times under a pressure of 1000 bar to obtain niobium containing a situation mushroom supercritical extract (hereinafter referred to as sample S4).

ingredient weight% A Phenyltrimethicone 5 glycerin 5 Cyclopentasiloxane 20 Beta-sitosterol 3 cholesterol 3 Methylparaben 0.2 Coles-24 / Setes-24 3 Hydrogenated Dressin One Die-cetylphosphate 0.4 Disodium ID 0.01 Butylated-hydroxytoluene 0.05 Tocopheryl Acetate 0.5 Propylene glycol Quantity B Sample S1 10 system 100

(Preparation of Trinexamic Acid-containing Niosome)

Sample S2 is added to phase B of Table 1, except that the content of tranexamic acid in niosomes is 10% by weight, except for adding tranexamic acid-containing niosomes (Sample S5). Was prepared).

Preparation of Niosomes Containing a Mixture of Tranexamic Acid and Sacral Mushroom Supercritical Extraction

Sample S3 is added to phase B of Table 1, except that tranexamic acid and supernatant mushroom supercritical extract in niosomes are added in an amount of 10% by weight. A niosome containing supercritical extract (hereinafter referred to as sample S6) was prepared.

(Test Example 1)

Melanin Production Inhibition Test

Melanocytes isolated from human tissues were primaryly cultured and passaged two times, and then cultured in 60 mm culture dishes at a concentration of 2 × 10 ⁴ cells / culture plate using RPMI 1640 medium. After confirming that the cells adhered to the dish, samples S1, S2, S3, S4, S5 and S6 were added for each concentration as shown in Tables 2a and 2b. Three days after the addition, the cells were washed three times with PBS, and then cells were detached with trypsin-EDTA to uniformly distribute 2 × 10 ⁵ cells. 100 μl of 1N NaOH solution was added thereto and treated at 37 ° C. for 12 hours to completely lyse the cells. Absorbance was measured at 490 nm for 500 μl of cells in which cells were completely lysed. In addition, absorbance was measured using the extract-free treatment as a control.

As a standard indicator, 10 mg of synthetic melanin (M8601, manufactured by Sigma) was dissolved in 10 ml of 1N NaOH to prepare a mother solution (1 mg / ml), followed by 700 µg / ml, 300 µg / ml, 100 µg / ml, Dilutions of 70 µg / ml, 30 µg / ml, 10 µg / ml, 7 µg / ml, 3 µg / ml, 1 µg / ml and 0.1 µg / ml were prepared and the absorbance was measured at 490 nm.

Using the measured absorbance, the amount of melanin reduction was calculated according to the following equation.

The results are shown in Tables 2a and 2b.

In Tables 2a and 2b, it can be seen that the niorosomal containing tranexamic acid and the situation mushroom supercritical extract according to the present invention has an excellent melanin reduction effect. In addition, it can be seen that it is preferable to use trinemic acid and niobium containing supernatant mushroom supernatant at 0.01 to 20% by weight.

(Test Example 2)

Collagen Biosynthesis Effect

Fibroblasts incubated in T-75 flasks were washed 2-3 times with PBS, the adhered cells were dropped using trypsin, and the cells were collected by centrifugation (Heraeus; Labofuge 400R) at 1500 rpm for 5 minutes. After centrifugation, the supernatant was discarded, and 10 ml of DMEM (10% FBS) was added to the collected cells and mixed evenly. Cell counts were measured using a hemocytometer, and 200 μl of cells of about 96 ea / well were dispensed into each well of 96 wells. When cells at about 50% of the well area were grown while incubating at 37 ° C. and 5% CO ₂ , the samples S1, S2, S3, S4, S5 and S6 were exchanged with new DMEM (FBS10%). Cells were cultured by adding each concentration as shown in 3b.

Collagen synthesis performance measurement kit (Procollagen Type-I C-peptide EIA kit (Takara, MK 101)) was used to confirm the collagen biosynthesis effect. For measurement using immunological methods, 100 μl of Ab-POD conjugate solution was transferred to one well and aliquoted, and 20 μl of cell culture or standard solution was added. A 96 well plate was wrapped in foil and incubated at 37 ° C. for about 3 hours. After 3 hours, all the solutions in each well were removed and washed 4 times with PBS. After the washing step, 100 μl of substrate solution was added to each well and incubated at 20-30 ° C. for 15 minutes. In the final step, 100 µl of 1N H ₂ SO ₄ , the reaction termination solution, was added and mixed gently. The absorbance was measured with an ELISA reader and the result was used. After the reaction solution was added, the plate was stored at room temperature for about 1 hour and absorbance was measured at 450 nm.

Synthesis (%) was calculated according to the following formula, and the results are shown in Tables 3a and 3b.

In the above Table 3, it can be seen that the nitric acid-containing trinexamic acid and the situation mushroom supercritical extract according to the present invention have excellent collagen biosynthesis by cell proliferation. In addition, it can be seen that it is preferable to use trinemic acid and niobium-containing mushroom supercritical extract at 0.01 to 20% by weight.

(Manufacture example 1)

To prepare a cosmetics according to the invention by mixing the components of Table 4.

(Comparative Production Examples 1 to 5)

To prepare a cosmetic by mixing the components of Table 4.

(Test Example 3)

Skin elasticity enhancing effect

Thirty healthy women over the age of 20 were divided into six groups at a temperature of 22-24 ° C and a relative humidity of 55% .Preparation Example 1 in Group A, Comparative Production Example 1 in Group B, Comparative Production Example 2 in Group C, Comparative Preparation Example 3 in Group D, Comparative Preparation Example 4 in Group E, and Comparative Production Example 5 in Group F, respectively, were formulated according to Table 5 below, followed by 12 weeks of application (2 times / day) around the eyes. Skin elasticity was measured using a skin elasticity measuring instrument (Cutometer SEM 575, C + K Electronic Co., Germany).

The experimental results are described in Table 5 below as the average value of each test group of the skin elasticity measuring instrument as ΔR5 value [R5 (12 weeks) -R5 (0 weeks)]. The value of R5 represents the actual modulus of elasticity, and closer to 1 indicates that the elasticity is better.

Skin elasticity enhancing effect (△ R5) Preparation Example 1 0.28 Comparative Production Example 1 0.10 Comparative Production Example 2 0.08 Comparative Production Example 3 0.11 Comparative Production Example 4 0.10 Comparative Production Example 5 0.12

In Table 5, the skin elasticity-enhancing effect of Preparation Example 1 containing the situation mushroom supercritical extract and Tranexamic acid-containing niosomes was 35.71% compared to Comparative Preparation Example 1, 28.57% compared to Comparative Preparation Example 2, Comparative Preparation Example 3 Compared with Comparative Preparation Example 4, it was 39.29%, 35.71% and 42.85%, respectively.

Therefore, the cosmetic composition containing the supernatant mushroom supercritical extract and tranexamic acid-containing niosomes according to the present invention is mixed with the supernatant mushroom supercritical extract and tranexamic acid and stabilized with niosomes, which are not stabilized with niosomes. Compared to the case of using the niobium containing supernatant extract and tranexamic acid or supernatant mushroom and tranexamic acid, respectively, the skin elasticity enhancing effect was obtained.

Experimental Example 4

Whitening effect by inhibiting melanin production

In order to investigate the effect of external skin on blemishes and freckles, Well Control Test was conducted according to the double blind method, and the results were analyzed using a color difference meter (Minolta, Chromameter CR-300). The value (brightness) was calculated.

As an experimental method, ultraviolet rays were irradiated to 0.88J / cm <2> three times once a day for 1 day, and manufacture example 1 and comparative manufacture examples 1-5 were applied twice a day for 30 days, respectively. The whitening effect according to L value before and after application was calculated according to the following formula.

The results are shown in Table 6 below.

Whitening Effect L (%) Preparation Example 1 94 Comparative Production Example 1 32 Comparative Production Example 2 28 Comparative Production Example 3 45 Comparative Production Example 4 38 Comparative Production Example 5 39

In Table 6, the cosmetic composition containing the situation mushroom supercritical extract and tranexamic acid-containing niosome according to the present invention, by stabilizing niosome by mixing the situation mushroom supercritical extract and tranexamic acid, niosome It is much more effective in preventing skin blackening as compared to the case of using niobium containing supernatant extract and tranexamic acid, or supersomnia extract and tranexamic acid, respectively. It was found that a whitening effect was obtained to remove stains.

Experimental Example 5

Formulation Stability Test

Samples stored in an opaque glass container for 12 weeks in a constant temperature bath at constant temperature maintained at 45 ℃, respectively, Preparation Example 1 and Comparative Production Examples 1 to 5, the opaque glass container in a completely shaded refrigerator maintained at 4 ℃ constant The separation and discoloration of the samples stored for 12 weeks and samples stored for 12 weeks in a circulation chamber circulating at -5 ℃ to 37 ℃ was measured.

The results are shown in Table 7 below.

At this time, the degree of product separation and discoloration were evaluated by classifying into the following six grades.

Product discoloration evaluation criteria:

0: no change 1: very little separation (discoloration)

2: Separate slightly (discolored) 3: Separate slightly (discolored)

4: severely separated (discolored) 5: extremely severely separated (discolored)

Temperature Preparation Example 1 Comparative Production Example 1 Comparative Production Example 2 Comparative Production Example 3 Comparative Production Example 4 Comparative Production Example 5 45 ℃ 0 2 0 0 0 One 4 ℃ 0 0 0 0 0 0 -10 ~ -50 ℃ 0 2 One 0 0 2

In Table 7, Preparation Example 1 according to the present invention was maintained stable in all the test conditions, Comparative Preparation Example 1 is 45 ℃, discoloration or separation symptoms in the circulation test, Comparative Preparation Example 2 is very little in the circulation test Dissociation and discoloration were unstable, and Comparative Example 3 showed dissociation in discoloration and circulation tests at 45 ° C. Accordingly, Comparative Preparation Example 1, Comparative Preparation Example 2, and Comparative Preparation Example 5, which were used directly without stabilizing the tranesamic acid and the situation mushroom supercritical extract, respectively, had a slight discoloration and separation in the circulating test, resulting in unstable extracts. And it was found.

(Manufacture example 2)

Lotion (skin lotion)

(Manufacture example 3)

Nourishing Cosmetic Lotion

(Manufacture example 4)

Concentrated Cosmetic Water (Essence)

(Manufacture example 5)

Nutrition Cream

(Manufacture example 6)

Massage cream

(Manufacture example 7)

Cleansing cream

(Manufacture example 8)

pack

According to the present invention, the anti-wrinkle effect due to collagen biosynthesis and skin elasticity and the whitening effect due to reduced melanin production are not only excellent, but also excellent in stability and safety, and excellent skin permeability. An extract-containing cosmetic composition can be obtained.

Claims (2)

A cosmetic composition comprising tranexamic acid and supernatant mushroom supercritical extract, containing 0.01-20.0% by weight, based on total weight of the composition. The method of claim 1, The niomesome containing the tranexamic acid and the situation mushroom supercritical extract, the weight ratio of the tranexamic acid and the situation mushroom supercritical extract 1: 0.1 ~ 10.0 mixture is contained in an amount of 0.001 ~ 15% by weight relative to the total weight of the niosome Cosmetic composition, characterized in that.