KR20040099183A - Long term peservation method of the original form of garden balsam througth vacuum treatment after rapid freeze-drying - Google Patents

Abstract

PURPOSE: Provided is a long term preservation method of the original form of garden balsam(flower, leaf, root and seed) through vacuum treatment after rapid freeze-drying to use aromatic, dyeing and antibacterial functions of garden balsam. CONSTITUTION: A long term preservation method of the original form of garden balsam(flower, leaf, root and seed) through vacuum treatment after rapid freeze-drying comprises the steps of: collecting petals, leaves, roots and seeds of garden balsam and selecting them within a hour; coating the selected petals, leaves, roots and seeds with 70% alcohol containing glycerol(1-4%) and glucose(2-4%), and betaine(1-4%), followed by surface-drying; placing the surface-dried petals, leaves, roots and seeds in a vacuum packing container, followed by rapid freezing with liquified nitrogen for 10-20 minutes; and transferring the frozen petals, leaves, roots and seeds to a freeze-drier of -40 deg. C, followed by freeze-drying at -80 deg.C until a moisture content of 3 w% and under 10mTorr of vacuum condition.

Description

Long term peservation method of the original form of garden balsam througth vacuum treatment after rapid freeze-drying}

The present invention is to place the balsam (petals, leaves, seeds and mixtures) on the surface of the liquid nitrogen to quickly freeze, and to prevent the frost and the color change such as fine cracks caused by the rapid freezing of the balsam, Glucose, sucrose, fructose, trehalose, etc.), antifreeze (glycerol, ethylene glycol, etc.) and osmoprotective agents (betaine, etc.) are mixed with a spray coating and air-dried at room temperature for 3-5 minutes, then various vacuum packaging containers After decorating in a glass bottle, acrylic container, desiccant, etc., place a sealing cap (rubber stopper) at the upper end of the container and let it stand on the upper end of the liquid nitrogen surface at minus 196 ℃. Prefreeze rapidly for 10 to 20 minutes. The pre-frozen balsam is transferred into a pre-adjusted lyophilizer below -40 ° C and freeze-dried under vacuum for 5-15 days until the moisture content reaches 3w% at the final -80 ° C vacuum level below 10 mTorr. Press the sealing cap (rubber stopper) in a pressurized manner to vacuum-pack the container to prevent Transformation while having a sterilizing effect, and to suppress the color change at any time. It is a method of long-term preservation in order to take advantage of the unique fragrance, complexing and antibacterial functions contained in the balsam.

After unpacking, it is possible to reprocess or hydrolyze the prototype without powder, and to dye it to nails, toenails and skin, as well as to preserve the seeds and genetic material (DNA level) almost permanently. . Actually growing balsams are difficult to preserve as raw. It is easy to change shape and deteriorate, so it is generally stored dry and distributed.

In general, there are natural drying, hot air drying, and freeze-drying methods, but natural drying takes time by drying in the sun or by natural wind, and the shrinkage of tissue occurs as the moisture evaporates and disappears, so the shape is severely reduced and the color changes. It is hard to be crushed, hardened and pulverized. Especially, it is easy to be contaminated with mold or various bacteria during drying period, and deterioration of quality and decrease of yield cannot be excluded.

Hot air drying is a method of heating, blowing and drying at 40-60 ° C., which causes a lot of color and shape changes, resulting in very low aesthetics. Recently, the method of manufacturing the freeze-dried blossoming mushroom of Korean Patent Publication No. 2003-0019500 is similar to the freeze-drying process, but the coating treatment method for suppressing color change is not described, and it is simply absorbed by preserving it in a special protective container. As it provides only a blocking function, it is difficult to maintain the original dry level (below 3w%) because a moisture absorption blocking method is not provided during the treatment. This is because the lyophilized sample is absorbed very quickly when exposed to the outside. The lyophilization of this method starts drying in the frozen state at low temperature only at the initial stage, but it is dried by warming to room temperature until complete drying. Because change also happens. In addition, since the lyophilized sample is sterilized by ultraviolet sterilization and sealed in a sealed container by compression, there is a risk of deterioration from harmful bacteria that are not sterilized inside. On the other hand, since the product is dried by natural air-drying the nail specialty stump powder of the Korean Utility Model Publication No. 20-0326070, there is a high time of preservation period because of the high risk of deterioration or corruption in any storage container.

The balsam products to date are dried products dried by hot air or natural wind drying or powdered powder products thereof. For this reason, dried or powdered products are suspicious of the content of balsam, even if the contents are 100% pure. In order to exclude this doubt and provide a beautiful aesthetic unique to the balsam, it is necessary to confirm it directly with the naked eye of the consumer. Therefore, it is trying to commercialize the pristine freeze-dried vacuum-packed balsam which can be stored for a long time almost permanently without deterioration. In order to eliminate the defects of products that are difficult to discolor and become discolored due to discoloration, and to change the original characteristics, and to take advantage of lyophilization, first of all, to minimize the color and appearance change and to prevent damage and damage, (Procedure for further vacuum packaging) The balsam decoration should be completed. Second, the rapid prefreezing treatment using liquid nitrogen evaporative cooling minimizes the change of appearance. Third,-freeze-dried under vacuum below 10 mTorr at -80 ℃. The present invention is intended to solve the problem of improving the aesthetics of the color and appearance by sealing and fragile fragile defects, and long term storage almost permanently, unless the opening is completely blocked by moisture absorption and decay.

The present invention relates to a long-term preservation method of round balsam using lyophilized vacuum packaging method. To provide a lyophilized vacuum-packed balsam that is almost permanently safe and preserved almost permanently to prevent color and morphological changes in order to provide aesthetic beauty without altering the functional properties of the coloring, fragrance and antimicrobial activity that balsam contains. will be.

In order to achieve the desired invention, the following process must be solved, and the industrial mass production must be possible.

1) The process of coating the balsam with the composition of the plant coating material containing the color of the balsam, the anti-corrosive agent, the osmotic pressure protecting agent, the disinfectant and the softening agent to prevent lyophilization and the appearance and damage.

2) The process of decorating in a container by mixing alone or mixed petals, leaves, roots and seeds in order to provide aesthetics without sticking to each other during freeze-drying

3) A process in which the balsam decorated in the container is preliminarily frozen in the vaporization cold of liquid nitrogen (-196 ℃) to minimize the color and shape change during the freezing process, thereby enabling industrial mass production.

4) Process of adjusting the temperature and vacuum level so that the vacuum lyophilizer is suitable for the freeze-drying operation of balsam.

5) Vacuum packaging the container at a level of 10 mTorr or less so that balsam during lyophilization is sterilized in a state where moisture content is dried below 3w%.

<Description of the symbols for the main parts of the drawings>

A. Coating process before lyophilization

B. Bottling Decoration Process

C. Rapid prefreezing using liquid nitrogen vaporization

D. Freeze Drying and Vacuum Processing in the Freeze Dryer

E. Vacuum Packed Balsam Prototype After Freeze-Drying

The present invention relates to a long-term preservation method of round balsam by using a lyophilized vacuum packaging method so that color and shape change does not occur to provide aesthetic beauty without altering the functional properties of coloring, fragrance, and antibacterial activity of balsam. It is to be preserved almost permanently to provide a pristine balsam. Freeze-drying method is known to preserve growing balsam without damage, but rapid freeze treatment in the process of freezing living cells is caused by the freezing of intracellular water and damage of intracellular tissue due to the increase of water volume. It causes deterioration. Therefore, in order to freeze the living cells without damage, pretreatment of the East Sea protection agent to control intracellular moisture is an essential task. Therefore, glycerol (1-4%) and glucose (2-4%) and osmotic protective agent betaine (1-4%), etc., alone or mixed, are appropriately contained to protect the color, appearance, and damage of balsam. A first step of protecting the east sea of the tissue by coating the coating material composed of a 70% alcohol solution and using the same to coat the balsam tissue before freezing;

To freeze-dried, balsams are not attached to each other, to provide aesthetics, petals, leaves, roots and seeds alone or mixed and decorated in a container. A second process of decorating separately containing alum, salt or foxtail to allow for salting;

In order to minimize the color and shape change during the freezing, the balsam decorated in the container in the second process is rapidly pre-frozen for 5 to 10 minutes in vaporized cold air (-30 to -60 ° C) of liquid nitrogen (-196 ° C). The third process to enable mass production

A cap (lid, rubber product) for later sealing is placed on the upper end of the quick-prelimed frozen balsam and decorated container (glass bottle, acrylic container or desiccated vacuum container) and cooled to the same temperature level in advance (-30 to -60 ℃), the balsam is transferred to the vacuum lyophilizer and the inside of the lyophilizer is processed to a vacuum of 10 mTorr (0.01mmHg) or less with a temperature of -80 ℃ and an automatic pump so that the water content is less than 3w% for 5 to 15 days. A fourth step of freeze drying as much as possible,

Pressurize the cap of the upper end of the container with a hydraulic press located in the lyophilizer at a level of 10 mTorr or less so that the balsam during lyophilization is dried under 3w% of moisture and vacuum-packed. After the fifth step of freeze-drying and vacuum packaging by introducing into the freeze dryer, the final lyophilized vacuum-packed balsam is permanently preserved even at room temperature with its functional characteristics intact.

In the fourth process, the desiccator type vacuum processing container is a top and bottom separation type composed of a transparent acrylic (or PP) material, as shown in Fig. 2, and a silicon (or rubber product) ring exists in the middle separation part to vacuum internal air after bonding. If it is removed at the level, it can be combined and sealed by the external air pressure, and at the bottom of the inside, a plastic shelf for decorating the balsam and the lower part of the container have a tube that can remove or let air in. The middle part is equipped with a vacuum cage and the outside is equipped with a valve to block air blemishes. It is a top and bottom type hermetically sealed container that can vacuum the balsam after lyophilization even by a manual pump.

The balsam prepared above is stored in a freeze-dried vacuum to provide a shape and color almost permanently aesthetic appearance, and when opened, can be used as a dyeing material by pulverizing into fine particles, compared to the mass of freeze-dried balsam Water, alum and salt are mixed at a ratio of 1.0: 8.0: 0.8: 0.6 (w / w), which can be woven together to instantly color nails, toenails and skin. Antibacterial function is also included.

The freeze-dried vacuum-packed balsam product is the first completed by the present inventors, and until now, dried products dried by hot air or natural wind, or powdered powder products of dry balsam. The present invention solves the defect of color and shape change in general dry products and can preserve the primitive balsam semi-permanently, so that anytime, anywhere can use the coloring, fragrance, and antibacterial function that balsam contains. In addition, during preservation, it provides aesthetic function to appreciate the original beauty of balsam everywhere. Especially, since it is preserved without denaturation of protein, molecular genetic material can be preserved for a long time. These primitive balsams themselves are accompanied by images such as love, innocence, and customs (the customs of red in the nails with balsams, which means that the enemy defeats the war, in the custom of Korean customs). It can also be used as a gift for a specific day, and it is freeze-dried because it contains complexing (functions such as printing and printing), fragrance (effective for mild pancreas and migraine), antibacterial (effective for eczema, athlete's foot, etc.), and antioxidant function. Balsam, which is vacuum-packed and semi-permanently preserved, can provide various functions at any time.

Claims (4)

In order to prevent color change, appearance, and breakage of balsam, glycerol (1-4%) and glucose (2-4%) and betaine (1-4%), such as osmotic pressure-protective agent, are appropriately contained. Discoloration preventing coating agent composed of 70% alcohol solution and using it to coat balsamic tissue before freezing to reduce color and appearance change due to damage caused by freezing of tissues To freeze-dried, balsams are not attached to each other, but the petals, leaves, roots and seeds are decorated alone or mixed in a container in order to provide a shape and color that is nearly circular in appearance, and alum relative to the mass of the balsams before freeze-drying. And salt is mixed in a ratio of 0.8: 0.6 (w / w) to be separately packaged to be contained in the container, or after opening the dog grass (coloring function as a substitute for alum and salt) by wrapping it in a container with balsam and decorating Packing method that can be squeezed together with the contents by watering (single quantity = 1: 8 compared to building balsam) to immediately use coloring (bio imprinting and printing) function that can stain nails, toenails and skin immediately A cap (lid, rubber product) for later sealing is placed on the upper end of the quick-prelimed frozen balsam and decorated container (glass bottle, acrylic container or desiccated vacuum container) and cooled to the same temperature level in advance (-30 to -60 ℃), the balsam is transferred to the vacuum lyophilizer, and the inside of the lyophilizer is vacuumed to below 10 mTorr (0.01mmHg) with the temperature of -80 ℃ and the automatic pump so that the water content is less than 3w% for 5 to 15 days. How to freeze dry When the vacuum treatment is manually processed in claim 3, the container is made of a transparent acrylic (or PP) material so that a silicon (or rubber product) ring is present in the middle separation part in a top and bottom separation type, and the internal air is exhausted after the combination. The vacuum gauge attached to the vacuum gauge was removed and attached to the vacuum gauge to visually check the vacuum level below 100 mTorr (0.1mmHg), and then locked with a valve to block air flow from the outside. To vacuum in combination with air