KR20030095958A - Preparation method of liquid agent for increasing immunity - Google Patents

Abstract

PURPOSE: Provided is a preparation method of a liquid type agent which increases immunity. The prepared composition has excellent stability not to generate precipitate, as well as solid solubility. CONSTITUTION: A preparation method of a liquid type agent for the increase of immunity is characterized by adding polysaccharides of the structural formula to a mixed solvent of a japanese apricot extract and water; adding at least one kind of additive; and heating and sterilizing the mixture.

Description

Preparation method of liquid agent for increasing immunity

The polysaccharide substance of the following formula is extracted, separated and purified from the culture medium and mycelium mixture in which BASDIOMYCETES is liquid cultured using the raw material of plant tissue as a medium raw material, and has an effect such as immunopotentiation. As a dietary supplement used as a supplement, it is widely marketed under the trade name AHCC. However, it has been pointed out that it is inconvenient to take because it is prepared in powder form, and taste and rejection of taking.

(Wherein R represents a hydrogen atom or an acetyl group, the hydroxyl group on the 2,3 position is about 30% acetylated and has a molecular weight of 500 to 1000)

In the conventional liquid herbal preparation, water or ethyl alcohol was used as the extraction solvent.In the National Institutes of Health regulations, the standard liquid solution was added with 10 times the amount of the total amount of the drug substance and extracted for 2 hours at 80 to 100 ° C. It is prescribed to extract until the amount reaches 1/2.

In the case of the polysaccharide, a liquid formulation is prepared in such a manner, and there is a lot of difficulty in preparing a liquid that is easy to take because it does not become a complete solution state such as a precipitate.

Accordingly, an object of the present invention relates to a process for preparing the polysaccharide in the form of a complete solution which does not form a precipitate even after long term storage.

The object of the present invention is achieved by a method for producing a liquid immuno-enhancing composition consisting of dissolving a polysaccharide of the following formula in a mixed solvent of plum extract and water and then adding an additive such as a sweetener.

(Wherein R represents a hydrogen atom or an acetyl group, the hydroxyl group on the 2,3 position is about 30% acetylated and has a molecular weight of 500 to 1000)

Plum extract added to the composition of the present invention is added in an amount to adjust the pH of the composition of the present invention in the range of 3-5. In addition, the sweetener is added in an amount such that the sugar content is 15-25%. Moreover, additives, such as various vitamin preparations, such as a vitamin B group, can be further added.

The liquid formulation prepared by the method for preparing the liquid composition of the present invention is suppressed the formation of prolonged precipitation and the physical stability of the dispersion system is also maintained for a long time has the effect of ensuring high commerciality and uniformity of efficacy as well as a simple operation. Since it can ensure the manufacturing time is shortened and there is an excellent effect that the manufacturing process is simplified without the need for expensive equipment such as a high speed centrifugal separator.

The following examples illustrate the invention in more detail, but these examples do not limit the scope of the invention.

Example 1

23 g of AHCC-SS (manufactured by Amino Up Kagaku, Japan), 20 g of oligosaccharide, 2 g of plum extract (54 Brix), and 155 g of purified water were mixed for 30 minutes, sterilized by heating to 100 ° C, and then 30 minutes at 70-80 ° C. Sterilize and fill each container with 20ml using a three-sided packaging machine.

Comparative Example 1

23 g of AHCC-S (manufactured by Amino Up Kagaku, Japan), 20 g of oligosaccharide, and 155 purified water were mixed for 30 minutes, then sterilized by heating to 100 ° C and sterilized for 30 minutes at 70-80 ° C. Fill the container with 20 ml each.

Example 2

23 g of AHCC-SS (manufactured by Amino Up Kagaku, Japan), 20 g of oligosaccharide, 2 g of plum extract, and 2 g of inositol 2 g of purified water were mixed for 30 minutes, sterilized by heating to 100 ° C, and then sterilized for 30 minutes at 70-80 ° C. Fill the container with 20 ml each by using a three-side packing machine.

Example 3

23 g of AHCC-SS (manufactured by Amino Up Kagaku, Japan), 20 g of oligosaccharide, 2 g of plum extract, 2 g of inositol, 1 g of citric acid, and 152 g of purified water were mixed for 30 minutes, and then sterilized by heating to 100 ° C., followed by 70-80 ° C. Sterilize for 30 minutes in a three-sided packaging machine to 20mL each container.

Example 4

23g of AHCC-SS (manufactured by Amino Up Kagaku, Japan), 20g of oligosaccharides, 2g of plum extract, 2.2g of inositol, 1g of citric acid, 1g of vitamin B1, 0.002g of vitamin B2, 0.008g of vitamin B6, and 151.738g of vitamin B6 After mixing for 10 minutes, sterilize by heating to 100 ℃ and sterilization for 30 minutes at 70-80 ℃ to fill the container 20ml each by using a three-side packaging machine.

Example 5

23 g of AHCC-S (manufactured by Amino Up Kagaku, Japan), 20 g of oligosaccharide, 2 g of plum extract, and 155 g of purified water were mixed for 30 minutes, then sterilized by heating to 100 ° C and sterilized for 30 minutes at 70-80 ° C. Fill the container with 20 ml each using a packaging machine.

Example 6

23 g of AHCC-S (manufactured by Amino Up Kagaku, Japan), 20 g of oligosaccharide, 2 g of plum extract, and 2 g of inositol 2 g of purified water were mixed for 30 minutes, sterilized by heating to 100 ° C., and then sterilized for 30 minutes at 70-80 ° C. Fill the container with 20 ml each by using a three-side packing machine.

Example 7

23 g of AHCC-S (manufactured by Amino Up Kagaku, Japan), 20 g of oligosaccharide, 2 g of plum extract, 2 g of inositol, 1 g of citric acid, and 152 g of purified water were mixed for 30 minutes, and then sterilized by heating to 100 ° C., followed by 70-80 ° C. Sterilize for 30 minutes in a three-sided packaging machine to 20mL each container.

Example 8

23g of AHCC-S (manufactured by Amino Up Kagaku, Japan), 20g of oligosaccharide, 2g of plum extract, 2.2g of inositol, 1g of citric acid, 1g of vitamin B1, 0.002g of vitamin B2, 0.008g of vitamin B6, 151.738g of vitamin B6 After mixing for 10 minutes, sterilize by heating to 100 ℃ and sterilization for 30 minutes at 70-80 ℃ to fill the container 20ml each by using a three-side packaging machine.

Experimental Example 1 Comparative Experiment of Soluble Solids by Solvent

1) Test solution: A supernatant obtained by centrifuging the liquid compositions of Example 1 and Comparative Example 1 at 3,000 rpm for 10 minutes.

2) Test method: Take 10 ml of each test solution, dry it to a constant quantity at 105 ° C, cool it in desiccator, and weigh.

3) Results: Three times each, an average value was obtained, and the results are shown in Table 1 below. The available solids increased in the case of the example compared to the comparative example.

Table 1

Example 1 Comparative Example 1 Available Solids (g / 10ml) 2.21 (± 0.012) 1.83 (± 0.018)

Experimental Example 2: Comparison of Stability by Solvent

Example 6 and each of the compositions of Comparative Example 1 in each of 10 10 ml of a colorless transparent vial and sealed, each of the composition of Example 1 and three samples of Comparative Example 1 was 12 months at room temperature, and the rest Each of the compositions of Example 1 and three samples of the compositions of Comparative Example 1 measured the start time of precipitation for 30 days at 50 ° C.

The stability test results of the product at 50 ° C. and room temperature are as shown in Table 2 and Table 3a, respectively.

TABLE 2

Example 1 Comparative Example 1 Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 Sediment Creation Start Date One One 3 21 21 20

As can be seen in Table 2, when the precipitation stability test was carried out daily in the precipitation stability test under 50 ° C harsh conditions, the composition of Comparative Example 1 using water as a solvent produced precipitate within 1 to 3 days, whereas mesyl extract The composition of Example 1 using water as a solvent showed excellent stability because no precipitation was formed until 20 days passed.

TABLE 3

Example 1 Comparative Example 1 Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 2 weeks - - - - - - 1 month - - - + + + 2 months - - - + + + 4 months - - - + + ++ 6 months - - - ++ ++ ++ 9 months - - - ++ ++ ++ 12 months + + - ++ ++ ++

No sedimentation

+ Generate small amount of precipitate

++ Create a large amount of precipitation

As a result of the stability test at a long time storage at room temperature, in the composition of Comparative Example 1 using a water solvent, a small amount of precipitation began to be produced within one month, and from 6 months, a large amount of mass precipitate precipitated in the lower part of the test tube. In contrast, in the composition of the present invention, there was no precipitation generation within 9 months, and a small amount of precipitation was observed at the beginning of 12 months, so the stability was excellent.

As can be seen in the experimental example, the composition prepared by the production method of the present invention is excellent in stability, no precipitate is produced, and the solubility of solids as a complete liquid formulation is excellent.

The immune enhancing composition of the above formula sold in solid form by the present invention can be prepared as a liquid preparation and can be easily taken. A sweetener, vitamin B, etc. can be added to prepare an excellent formulation in terms of effects such as taste and immune enhancement. It became possible.

Claims (4)

The polysaccharide substance of the following structural formula is added to a solvent in which mesyl extract and water are mixed, and at least one additive is added and mixed, followed by heat sterilization. (Wherein R represents a hydrogen atom or an acetyl group, the hydroxyl group on the 2,3 position is about 30% acetylated and has a molecular weight of 500 to 1000) The method of claim 1, wherein the additive is a sweetening agent, a flavoring agent, or a method for producing a liquid immune enhancing composition, characterized in that the vitamin B complex. The method according to claim 1 or 2, wherein the extract of plum is added in an amount such that the pH of the composition is 3-5. The method of claim 1 or 2, wherein the sweetener is added so that the sugar content of the composition is 15-25%.