KR20030086187A - Antiageing cosmetics containing natural extracts - Google Patents

Abstract

PURPOSE: An anti-aging cosmetic composition containing an extract of Polia or Bombycis Corpus as an effective ingredient is provided which has safety for the skin and improves skin wrinkles, promotes collagen biosynthesis and has whitening effect on the skin. CONSTITUTION: An anti-aging cosmetic composition contains 0.0001 to 10% by weight of an extract of Polia or Bombycis Corpus, based on the total weight of the composition. Polia and Bombycis Corpus are ground, extracted in a solvent, filtered and concentrated in a distiller having a cooling condenser under reduced pressure. The solvent comprises C1-4 anhydrous or hydrous lower alcohol, a mixture of water and lower alcohol, a polar solvent such as acetone, 1,3-butylene glycol, n-propanol, n-butanol and a mixture thereof. The cosmetic composition increases the skin activity.

Description

Anti-aging cosmetics using natural products {Antiageing cosmetics containing natural extracts}

The present invention relates to anti-aging / whitening cosmetics containing herbal extracts with excellent blemishes, freckles improvement and wrinkle improvement, and elasticity enhancing effect of the face. More specifically, Baekjap and Baekbokryeong having excellent melanin inhibitory effect and collagen biosynthesis promoting effect. The present invention relates to an excellent dermatological drug and cosmetics containing a sequentially extracted from a polar solvent to a low polar solvent as an active ingredient.

Skin is the body's primary protective film that protects internal organs from changes in temperature and humidity, and from stimuli from the external environment such as ultraviolet rays and pollutants, and plays an important role in maintaining homeostasis such as body temperature control. However, cell damage stimuli, such as excessive physical and chemical stimuli and stress, malnutrition, ultraviolet radiation, environmental pollutants, and sudden changes in temperature, applied from outside the cell, induce a variety of biochemical changes in the cell, causing a cellular damage response. Among these, skin aging is recognized to be due in part to changes in the physiological function of the skin resulting from the oxidative damage response caused by these external stresses. In other words, if the cell membrane of skin cells is continuously damaged by free radicals or other radicals, the cross-linkage of protein or lipoprotein is increased or degraded, and the amount of lipid peroxide is increased. When the cell and the cell membrane are damaged, the permeability of the cell membrane is changed, and the ion balance in the cell is broken, and this change prevents the release of metabolic debris in the cell and deteriorates the cell's ability to detoxify the metabolic debris. If such cellular level changes persist, the tissue structure loses regularity. At the same time, individual cells grow relatively, while the total number of cells decreases. Local scars appear on the dermis, and the thickness of the dermis decreases due to the reduction of collagen fibers or elastic fibers. Crosslinking between collagen fibers also increases and the fine structure of the elastic fibers is also damaged, resulting in a decrease in the elasticity of the skin as a whole. Factors influencing this include hydrogen peroxide (H ₂ O ₂ ), superoxide anion, and hydroxy radicals. These harmful free radical species are involved, which in turn lowers the elasticity of the dermis and at the same time affects skin degradation. do. In other words, oxidative damage of skin cells by external stimuli ultimately promotes aging and degradation of the skin.

Eventually, the appearance of the skin promotes skin problems such as blemishes, freckles, blemishes, loss of elasticity, keratinization, and wrinkle formation and aging. In order to prevent such a phenomenon and maintain a healthier and more elastic skin, various kinds of animals, plants, Efforts have been made to effectively inhibit skin aging by maintaining skin's intrinsic function and activating skin cells by using cosmetics containing bioactive substances obtained by microorganisms and organic synthesis.

As such substances, vitamins such as retinol and ascorbic acid, proteins and flavonoids extracted from various plants and animals, amino acids, epidermal growth factor, alpha hydroxy acid and the like are known, and are currently used in cosmetics. However, most of these ingredients are known to be unstable by themselves or cause some irritation to the skin.

In addition, natural extracts, which are widely known in the folklore, have a small amount of active ingredients and vary in content depending on the place of origin or harvest, making it difficult to achieve homogeneity of quality.

In addition, various compounds are extracted together and have disadvantages in skin side effects and color. In order to overcome this point, purification is required, but the cost is high, and there is a problem in that it is not applied to actual mass production.

Therefore, researches on raw materials having an anti-aging effect on the skin without causing side effects on the skin have been actively conducted, and the present inventors have a problem with conventional anti-aging substances targeting various natural products that have been used as herbals. As a result of overcoming research and developing anti-aging agent with superior efficacy, it has been found that Baekbokryeong and Baekkang sleep extract have whitening / anti-aging effects, while developing purification methods to maximize its safety and efficacy, The present invention was completed by knowing the synergy effect of the combination. Accordingly, the present invention provides a cosmetic composition for preventing skin aging, which contains white baekbokryeong and baekpyeong, which are excellent in whitening / anti-aging effect, sequentially as an active ingredient.

It is an object of the present invention to provide a cosmetic composition containing Baekbokryeong and Baekjagyeong extract as active ingredients. In another aspect, an object of the present invention is to provide a cosmetic composition having a skin improving and / or activating effect. In another aspect, an object of the present invention is to provide a cosmetic composition having a wrinkle improvement effect, collagen biosynthesis promoting effect, whitening effect

Accordingly, the present invention provides a skin anti-aging composition containing Baekbokyeong and Baekjap, which are excellent in whitening and antiwrinkle effect, sequentially extracted from a polar solvent with a low polar solvent as an active ingredient.

Baekgangjam is one of the herbal medicines that have been widely known and used not only in oriental medicine but also in folk medicine. White squirrel refers to the dead body of silkworm moth and silkworm moth, which is an animal, and died of sclerotic disease. Its main ingredients are lipoprotein and minerals. It has been used to whiten pigments such as blemishes since ancient times, and it is known to have an excellent effect on the prevention of aging. In Korean medicine, white nap is dizziness due to a stroke, nasal congestion and itchy skin, headache, vomiting, mouth and eyes of the stroke. When crooked, it was used for tetanus and the like.

Baekbokryeong is a white or grayish white mycelium that parasitizes pine roots in soil 5 to 9 years after logging pines. It helps to hydrate metabolism smoothly, it is effective for blemish skin and aging skin, and helps blood circulation smoothly. Its main ingredients include β-Pachymose, Pachymic acid, organic acids, proteins, fats, minerals and Lecithine. In oriental medicine, it is used to strengthen the spleen, nourish the metabolism and all cells, and activate brain cells to stabilize the mind and improve weakness. It helps to circulate blood waste and helps to circulate forgetfulness and guanwasa.

In the present invention, the first extract of Baekbokryeong and Baekjagi from anhydrous or lower alcohols, acetone, and second extraction with low polar solvents such as ethyl acetate, diethyl ether, benzene, methylene chloride, chloroform, hexane It is added to cosmetics, that is, supple cosmetics (skin), nourishing cosmetics (lotions), nourishing creams, massage creams, essences, and packs.

The present invention will be described in more detail as follows. Buy baekbokryeong and baekpzeum, which are commercially available as herbal medicines, and finely grind them. Extracted solvents are water, lower alcohols (methanol and ethanol), mixtures of water and lower alcohols, acetone, and 1,3-butylene glycol (Butylene). 5-20 times the volume of polar solvents such as glycol, n-Propanol, iso-propanol, and n-butanol, and mixed solvents were added by dipping, filtration, and filtration. The filtrate is concentrated under reduced pressure. After dispersion by adding water to the concentrate, an equal amount of low polar organic solvent, ie, ethyl acetate, diethyl ether, benzene, chloroform, etc., is added to the dispersion and shaken. The organic layer is separated, treated with activated carbon, and concentrated under reduced pressure to obtain an excellent extract. Extraction method includes cooling condenser installed to prevent evaporation of active ingredient, in case of cold acupuncture and percolation about 12-96 hours, or 1-4 anhydrous or hydrous lower alcohol, ethyl acetate or dimethyl The active ingredient may be extracted by aging for 3-20 days at 4-25 ° C. at room temperature using ether as a solvent. In the case of a warm needle, it is different depending on the type and temperature of the solvent and the like to be used, but it is preferable to perform about 5-24 hours at a temperature close to the reflux temperature of the solvent. After the extraction is completed, the resultant is filtered with a filter cloth, and the residue is further extracted one or more times in the same manner. The primary extract is dispersed in water and then extracted twice with a low polar solvent. The combined organic layers were combined, treated with activated carbon, dried over anhydrous sodium sulfate, concentrated under reduced pressure, and lyophilized or spray dried to obtain a dry extract. Dried Baekbokryeong and Baekjam extracts show very strong activity inhibitory effect and collagen synthesis promoting effect when treated with human tyrosinase compared to conventional polar solvents.

Baekbokryeong and Baekjapeng extract obtained as described above are manufactured with cosmetics such as skin ointment or lotion, and when applied to human skin, the skin whitening effect and wrinkle improvement effect are very excellent. In addition, it does not cause any side effects, and it can be seen that Baekbokryeong and Baekjam extracts are safe and effective, improving blemishes and freckles and improving skin wrinkles.

In addition, in the combination of Baekbokryeong and Baekkang sleep extract of the present invention, the combination ratio of Baekbokyeong and Baekjam sleep extract is preferably 1: 1 to 2: 3. The content of Baekbokryeong and Baekjapeng extracts contained in the preparation of external skin ointments or cosmetics is appropriately 0.0001-10.0 weight, preferably 0.0005-5.0 weight in dry weight. A clear effect could not be expected at concentrations below 0.0001 weight, and no increase in effect was seen at concentrations above 5 wt.

The composition according to the invention contains cosmetically and dermatologically acceptable media and / or bases. These are all formulations suitable for topical application, for example emulsions, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) obtained by dispersing an oil phase in a solution, gel or solid or pasty anhydrous product, aqueous phase and / or Or in the form of a nonionic vesicle dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or cone stick. These compositions can be prepared according to conventional methods in the art. The composition according to the invention can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

Compositions of the present invention include fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers. Cosmetics such as fillers, metal-ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics or It may contain adjuvants commonly used in the field of dermatology. These adjuvants are introduced in amounts commonly used in the cosmetic or dermatological field.

Next, the present invention will be described in detail with reference to Examples. However, it is apparent that the present invention is not limited by the examples.

Example 1

50 g of Baekbokryeong powder was added to 1 liter of a 50% ethanol aqueous solution, and extracted by boiling in an extractor equipped with a cooling condenser for 12 hours, filtered through a 300 mesh filter cloth, and the filtrate was left at 4-15 ° C. for 7 days to mature. Filter with filter paper once. The extract is concentrated under reduced pressure at 60 ° C. using a distillation apparatus equipped with a cooling condenser. This was dispersed in 50 ml of water and extracted again with 300 ml of ethyl acetate. 10 g of activated carbon was added thereto, stirred at room temperature for 1 hour, and filtered. The filtrate was dried with anhydrous sodium sulfate, filtered and concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser and dried to obtain a dry weight of 2.0 g.

Example 2

50 g of white nap powder was added to 1 liter of ethanol, boiled in an extractor equipped with a cooling condenser for 12 hours, extracted, filtered through a 300 mesh filter cloth, and the filtrate was left at 4-15 ° C. for 7 days to mature, and then filtered through Whatman No. 2 filter paper. To remove insoluble matter. The extract is concentrated under reduced pressure at 60 ° C. using a distillation apparatus equipped with a cooling condenser. This was dispersed in 50 ml of water and extracted again with 300 ml of ethyl acetate. 10 g of activated carbon was added thereto, stirred at room temperature for 1 hour, and filtered. The filtrate was dried over anhydrous sodium sulfate, filtered, and the organic layer was concentrated and freeze-dried to obtain a dry weight of 2.4 g.

Example 3

50 g of Baekbokryeong flour was added to 1 liter of normal butanol and boiled in an extractor equipped with a cooling condenser for 12 hours, followed by filtration with a 300 mesh filter cloth. Filter to remove insoluble matter. The extract is concentrated under reduced pressure at 80 ° C. using a distillation apparatus equipped with a cooling condenser. This was dispersed in 50 ml of water and extracted again with 300 ml of methylene chloride. 10 g of activated carbon was added thereto, stirred at room temperature for 1 hour, and filtered. The filtrate was dried over anhydrous sodium sulfate, filtered, and the organic layer was concentrated and freeze-dried to obtain a dry weight of 2.7 g.

Example 4

50 g of white bran powder was added to 1 liter of normal butanol, and extracted by boiling in an extractor equipped with a cooling condenser for 12 hours, and then filtered through a 300 mesh filter cloth. Filter to remove insoluble matter. The extract is concentrated under reduced pressure at 80 ° C. using a distillation apparatus equipped with a cooling condenser. This was dispersed in 50 ml of water and extracted again with 300 ml of methylene chloride. 10 g of activated carbon was added thereto, stirred at room temperature for 1 hour, and filtered. The filtrate was dried over anhydrous sodium sulfate, filtered, and the organic layer was concentrated and freeze-dried to obtain a dry weight of 2.8 g.

Example 5

50 g of each of the Baekbokryeong and Baekjapje powders, which were granulated by Baekbokryeong and Baekseomjam, were put into 1 liter of a 50% ethanol aqueous solution, boiled for 12 hours in an extractor equipped with a cooling condenser and filtered with 300 mesh filter cloth. After aging for one day, it is filtered with Whatman No. 2 filter paper. The extract is concentrated under reduced pressure at 80 ° C. using a distillation apparatus equipped with a cooling condenser. Disperse it in 100 ml of water, add 3 liters to a separatory funnel, add 1 liter of ethyl acetate, shake well, mix well, separate completely into two layers, and take the upper layer (ethyl acetate layer). Put the lower layer (water layer) back into the separating funnel and extract twice more with ethyl acetate. The combined upper layers were combined, 20 g of activated carbon was added, stirred at room temperature for 1 hour, and filtered. The filtrate was dried over anhydrous sodium sulfate, filtered, concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser, and dried to obtain a dry weight of 5.2 g.

Example 6

50 g of each of Baekbokryeong and Baekjap, finely ground Baekbokryeong and Baekjap, were added to 1 liter of ethanol, boiled for 12 hours in an extractor with a cooling condenser, filtered, and filtered through a 300 mesh filter cloth, and the filtrate was left at 4-15 ° C for 7 days. After aging, the filter is filtered through Whatman No. 2 filter paper to remove insoluble matters. The extract is concentrated under reduced pressure at 60 ° C. using a distillation apparatus equipped with a cooling condenser. After dispersing this in 100 ml of water and extracting three times with 1000 ml of methylene chloride, 20 g of activated carbon was added thereto, stirred at room temperature for 1 hour, and filtered. The filtrate was dried over anhydrous sodium sulfate, filtered, and the organic layer was concentrated and freeze-dried to obtain a dry weight of 5.1 g.

Example 7

50 g of each of the Baekbokryeong and Baekseom powders, which were granulated by Baekbokyeong and Baekseom, were added to 1 liter of normal butanol, boiled in an extractor equipped with a cooling condenser for 12 hours, extracted, and filtered through a 300 mesh filter cloth. After aging, it is filtered through Whatman No. 2 filter paper to remove insoluble matters. The extract is concentrated under reduced pressure at 80 ° C. using a distillation apparatus equipped with a cooling condenser. After dispersing this in 100 ml of water and extracting three times with 1000 ml of methylene chloride, 20 g of activated carbon was added thereto, stirred at room temperature for 1 hour, and filtered. The filtrate was dried over anhydrous sodium sulfate, filtered and the organic layer was concentrated and lyophilized to obtain a dry weight of 4.9 g.

Comparative Example 1

50 g of Baekbokryeong flour was added to 1 liter of ethanol, extracted by boiling in an extractor equipped with a cooling condenser for 12 hours, filtered through a 300 mesh filter cloth, and the filtrate was left to mature at 4-15 ° C. for 7 days, and then filtered through Whatman No. 2 filter paper. do. The extract was concentrated under reduced pressure at 50 ° C. using a distillation apparatus with a cooling condenser and dried to obtain a dry weight of 3.4 g.

Comparative Example 2

50 g of white nap powder was added to 1 liter of ethanol, boiled for 12 hours in an extractor equipped with a cooling condenser, filtered, and filtered through a 300 mesh filter cloth. The filtrate was left at 4-15 ° C. for 7 days to ripen, and then filtered through Whatman No. 2 filter paper. do. The extract was concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser and dried to obtain a dry weight of 3.5 g.

Comparative Example 3

50 g of each of the Baekbokryeong and Baekjapje powders, which were granulated by Baekbokryeong and Baekjap, were added to 1 liter of ethanol, boiled for 12 hours in an extractor equipped with a cooling condenser, filtered, and filtered through a 300 mesh filter cloth. After aging, the filter is filtered using Whatman No. 2 filter paper. The extract was concentrated under reduced pressure at 50 ° C. using a distillation apparatus equipped with a cooling condenser and dried to obtain a dry weight of 6.9 g.

The present inventors conducted experiments on skin safety and improvement / or activation effect on Baekbokryeong and Baekkang sleep extract. It was shown by the following experiment that Baekbokryeong and Baekgwa extracts had anti-wrinkle effect, collagen biosynthesis promoting effect and human tyrosinase activity inhibiting effect.

Test Example 1 Safety of Baekbokryeong and Baekkang Sleep Extract

Since raw materials for cosmetics are used in the human body, safety for the human body is important above all. The present inventors confirmed that the raw materials without toxicity and irritation as cosmetics through the following experiments on the presence or absence of toxic and irritant to the human body of Baekbokryeong and baekgangjam extract. Safety experiments were performed with the solution of Example 7 in a 10% concentration in squalane.

1-1] Acute oral toxicity test in mice: 1 ml / Kg of test substance was administered to 10 rats of 5 males and 5 females, and no dead animals were observed. Not observed.

1-2] Acute dermal toxicity test in mice and rabbits: 0.2 mL / Kg of test substance was administered once percutaneously to 10 rats of 5 males and 5 females. Results No abnormal symptoms were observed in the pre-administration group. No abnormalities were observed in the rabbits in the same manner.

1-3] Primary skin irritation test: Twelve rabbits were removed 24 hours before application of test substance and observed by applying 0.1 mL each for 2.5 hours at 2.5cm × 2.5cm width. . Observation determined that there was no stimulus.

1-4] Eye irritation test: 9 rabbits were diluted in saline at 2% concentration and administered to the eye 0.1 mL per animal. Experimental results showed no specific eye mucosal irritation to cornea, iris and conjunctiva.

1-5] Skin sensitization test: Skin sensitization test was performed in six of each of three male and female gunea pigs according to Magnusson's and Kligman's test methods.

Human patch test: Thirty healthy women aged 20-28 years were tested according to the CTFA Guideline (The Cosmetic Toiletry and Fragrance Association, INC, Washington, D. C. 20036, 1991). Results There was no skin primary irritation response.

1-7] Repeat Insult Human Patch Test: As a result of the CTFA guideline, the human patch test did not show cumulative stimulus response or sensitization response.

In the above toxicity and skin safety experiments, it was confirmed that Baekbokryeong and Baekjapgi extracts were safe as external preparations for skin.

Test Example 2 Wrinkle Improvement Effect

Examples of anti-wrinkle and baekbokryeong baekgangjam extract effective to see the proliferative skin cells (skin cell) effect in each well (Well) of the human normal fibroblast cells (Human Fibroblast Nomal) the 96-well microtiter plates (96-well microplate) 1x10 ⁴ Inoculate the cells to incubate for 24 hours in DMEM (Dulbecco's Modified Eagle's Medium) medium. After culturing, Example 1-7 and Comparative Example 1-3 prepared above were replaced with a serum-free DMEM medium adjusted to a final concentration of 250 μl / ml, followed by further incubation for 24 hours. 3- (4,5-dimethylriazol-2-yl) -2,5-diphenyltetrazolium (MTT: (3- (4,5-dimethyl-thiazol-2-yl) 2,5-diphenyl tetrazolium: 5 mg / ml) was added 10 µl, and the media was discarded after 4 hours, and 100 µl of dimethyl sulfoxide (DMSO) solution was added to each well, and the absorbance at 570 nm was measured using a microplate reader. By performing the above procedure, the cell proliferation effect was obtained from the following equation, and the results are shown in Table 1.

Cell proliferation effect (%) = {(absorbance at extract treatment-absorbance of control) / absorbance of control} X 100

Table 1 Cell Proliferation Effect

As shown in Table 1, it can be seen that the cell proliferation effect is excellent in normal human fibroblasts to which the Baekbokryeong and Baekjapgi extracts of the present invention are added.

Experimental Example 2 Collagen Biosynthesis Promoting Effect

To investigate the effects of Paekbokyeong and Paekjong extract on the skin, the effects of promoting collagen biosynthesis were examined in vitro. In the test method, human normal fibroblasts (Human Normal Fibroblast) are seeded in 24 wells of 10 ⁵ per well and incubated until development of about 90%. This was washed once with PBS, and then treated with Baekbokryeong and Baekjap extract of Examples 1 to 7 and Comparative Examples 1-3 prepared above, and incubated in a CO ₂ incubator for 24 hours. The supernatant was removed and the amount of procollagen type (1) C-Peptide (PICP) was increased. The test results are shown in Table 2 below. Synthetic performance is prepared by setting the control (untreated group) to 100.

[Table 2] Collagen biosynthesis promoting effect

Collagen is a biomaterial that accounts for most of the skin's connective tissue. It is related to skin elasticity and decreases with age. Therefore, increasing the amount of collagen biosynthesis can increase the elasticity of the skin.

Experimental Example 3 Inhibitory Effect on Human Skin Pigment Cell Tyrosinase Activity

Enzyme tyrosinase used human tyrosinase. The activity of the enzyme was measured at 475 nm to produce DOPA chrome, the browning product of L-DOPA. 76ul of 50mM Tris-HCl buffer solution (pH 7.5) was added to the test tube and maintained at 37 ° C. Then, 20mM L-DOPA 190ul, 50m of enzyme solution and 40ul of whitening activity target sample were added, mixed well, and the absorbance change was measured at 475nm. At this time, the measurement is performed without the target sample. When the enzyme activity of the control was seen as 100, each enzyme inhibition activity was expressed in% compared to the control. The relative activity of arbutin widely used in whitening cosmetics was compared to observe the relative strength of tyrosinase inhibitory activity of Baekbokryeong or Baekjapgi extract.

Tyrosinase Inhibitory Activity (%) = (absorbance / Control Absorbance at Sample Addition) by Subject Feed × 100

TABLE 3 Tyrosinase Inhibitory Activity

As shown in Table 3, Baekbokryeong and Baekgam sleep extracts showed inhibitory effect on human tyrosinase activity.

The present invention will be described in more detail by illustrating the preparation of the following formulations by introducing the above cosmetically effective Baekbokryeong and Baekjapgi extract into the solubilizer type and the emulsifier type, respectively, but the present invention is not limited to these preparation examples.

[Production example]

Preparation Example 1 to 3 Emulsion Formulation

Table 4 Composition of Emulsifier

Preparation Examples 4 to 6 Solubilized Formulations

[Table 5] Composition of the solubilizing agent type

The storage stability as a cosmetic was evaluated for the formulations of Preparation Examples 1 to 6 prepared above. In order to confirm the storage stability, the stability of each temperature was investigated, and the results are shown in Table 6.

[Table 6] Storage Stability by Temperature

From Table 6, it can be seen that the formulation according to the present invention has excellent storage stability.

As described above, the cosmetics containing Baekbongryeong and Baeksijam extract of the present invention is excellent in wrinkle improvement effect, collagen biosynthesis promoting effect, whitening effect and safe for skin.

In addition, the cosmetics containing the Baekbokryeong and Baekjapgi extract of the present invention can be provided as a water-soluble cosmetics or emulsified cosmetic formulation, when formulated was also excellent storage stability.

Claims (3)

An anti-aging cosmetic composition comprising, as an active ingredient, 0.0001-10% by weight of Baekbokryeong and Baekjapgi extract, based on the total weight of the composition. The method according to claim 1, wherein the Baekbokryeong and Baekjang extract are crushed Baekbokryeong and Baekja, then anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol), a mixture of water and lower alcohol, acetone, 1,3-butyl Deposition extraction by adding 5-20 times the volume of polar solvents such as butylene glycol, n-Propanol, iso-propanol, and n-Butanol and their mixed solvents After filtration and concentration under reduced pressure, the extract obtained by using ethyl acetate or ethyl ether as a solvent was treated with activated charcoal, and concentrated under reduced pressure to a suitable temperature in a distillation apparatus equipped with a cooling condenser. Cosmetic composition comprising the prepared Baekbokryeong and Baekkang sleep extract. According to claim 1, Cosmetic composition, characterized in that to improve the wrinkles, collagen biosynthesis promoting effect, whitening effect to increase the skin vitality.