KR20030083251A - Extracts of Chaga Fungus And Stomach Inflammatory Agent Containing The Same - Google Patents

Abstract

PURPOSE: Provided are an extract of Inonotus Obliquus and a therapeutic agent for gastritis containing the same. The extract of Inonotus Obliquus is applied to the prevention and treatment of stomach ulcer. CONSTITUTION: A pharmaceutical composition for the treatment of gastritis and stomach ulcer is characterized by containing an extract of Inonotus Obliquus as an active ingredient. The extract is orally administered three times per day within the range of 100-5000mg.

Description

Extract of Black Birch Mushrooms and Agent for Gastritis Containing It {{Extracts of Chaga Fungus And Stomach Inflammatory Agent Containing The Same}

The present invention relates to a pharmaceutical composition containing an extract of black birch mushroom as an active ingredient, which has a therapeutic effect against acute gastritis and gastric injury and removes excess gastric acid.

Korean people have many digestive diseases such as gastritis and gastric ulcer due to eating habits, and these diseases have high recurrence rate after initial treatment. Peptic ulcer is a disease in which a circular, elliptical or linear ulcer develops in a part of the gut mucosa. It is called peptic ulcer because it causes a strong digestive gastric juice to contact areas, namely the lower esophagus, gastric antrum, proximal part of the duodenum, McCell diverticulum and gastrointestinal sutures. Most cases occur in the stomach and duodenum, so it is generally called stomach or duodenal ulcer.

Pathologically, the presence of ulcers in the mucosal areas of the stomach and intestine is called hemorrhagic erosion or acute superficial ulcer. Superficial ulcers reach muscularis mucosa and pass through submucosa to muscularis. In severe cases, the damaged area reaches the serosa and gastric fluid leaks into the abdominal cavity, causing severe pain and requiring surgical intervention. Peptic ulcers can cure naturally without any symptoms, but most ulcer patients complain of heartburn or sintrim.

Pepsin (pepsin) is a digestive enzyme that can digest almost any kind of protein ingested by meal. It is most active under acidic conditions, and this environment is exposed to hydrochloric acid (HCl) in gastric juice. Provided by When gastric ulcers occur due to the various causes mentioned, pepsin acts on the ulcer site while digestion is in progress in the stomach, causing severe pain by acting as hydrochloric acid, a strong acid.

Peptic ulcers are caused by a number of factors, including mental and physical stress, medications (primarily anti-inflammatory drugs), and traumas that break down the equilibrium between homeostasis and attackers on the gastrointestinal mucosa. Attack factors include hydrochloric acid secreted from parietal cells of gastricglands and pepsin, a digestive enzyme secreted from chief cells. The acid in the gastric juice plays an important role as an attacker of ulcers. Most patients with stomach and duodenal ulcer are accompanied by symptoms such as sintrim due to excessive stomach acid. Bile acid in bile is recognized as an attack factor because it damages the gastric mucosa like alcohol or anti-inflammatory analgesics, or destroys the mucose barrier that prevents mucosal damage by the release of secreted hydrochloric acid into the mucosa. Protective factors include mucosa, mucin that surrounds the surface of the gut mucosa, blood flow in the mucosa, and prostaglandin (PG), which constitutes the mucosa.

In general, peptic ulcer disease is stabilized and the symptoms are promptly improved by taking medication, but it is known that the recurrence is difficult to cure frequently. It is also a factor unless the patient stops taking medication because it is judged that the patient has completed the self-recognition symptom and stops taking the medication. It is true. There are many causes of all illnesses, but the cause of the disease is explained by the imbalance between the attacker and the defender. No matter how strong the attacker is, even if the defender is strong, the disease does not occur. Also, if you are poor, you will get sick. Thus, the essence of medicine in the treatment of diseases is to construct a rational formula that enhances the defensive factor and at the same time disables the attacker.

The pain-causing factor in most ulcers is gastric acid (hydrochloric acid), which is a strong acid with a pH of 1-2, which is essential for digesting protein, the main nutrient. The stomach wall of a healthy person is surrounded by mucous membranes and is protected from gastric acid. However, if the erosion or ulceration occurs on the gastric mucosa due to the causes listed above, hydrochloric acid acts on these areas and the wound becomes worse and painful.

Most of the ulcer treatments used to date have been based on drugs such as antacids and acid secretion inhibitors because of the action of hydrochloric acid. As long as a person is alive, gastric acid will continue to be secreted and if ulcers are formed by this acid, you will have to take acid suppressants or antacids for life. However, these drugs only control the hydrochloric acid attacking the ulcer rather than treating the ulcer, so you can feel a bit of comfort only when you stop taking it. Let's do it.

The antacids commonly used in these peptic ulcers, sodium bicarbonate and calcium carbonate, have strong antacid abilities, but secondary acidic stimulation of the stomach wall by carbon dioxide generated after gastric acid neutralization produces more acid in the stomach. High doses of sodium and calcium have side effects such as alkalosis, hypercalcemia and hypertension. In addition, the acid secretion inhibitor (H ₂ antagonist) cimetidine suppresses the central nervous system in the elderly, and has an anti-androgen effect, which causes side effects such as breast enlargement and decreased libido and other drugs. It also increases the toxicity.

The antacid (Antacid), H ₂ inhibitors (H ₂ antagonist), proton pump blockers (Proton pump inhibitor), the gastric mucosa protective agent (Cytoprotective), etc. have been developed, but and improved and further the effect of side effects and accumulation operation for the long-term use for As the need for safe drug development increases, efforts are being made to develop the drug with drugs applied in private or oriental medicine.

The inventors of the present invention studied the physiological activity of black birch mushroom, while the extract of black birch mushroom protects the gastric mucosa from acute gastritis and gastrointestinal damage, protects stomach damage from excessive secreted gastric acid, and damages the stomach. It has been found to help restore the membranes of mucous membranes.

Therefore, in one aspect, the present invention provides a method for producing an extract of black birch mushroom, characterized in that the extract is obtained by hydrothermally extracting the black birch mushroom.

In another aspect, the present invention provides a therapeutic agent for acute gastritis and ulcers containing an effective amount of extract of black birch mushroom.

The present invention relates to a pharmaceutical composition containing a hot water extract of Black Birch Mushroom (Inonotus Obliquus) as an active ingredient, which has a therapeutic effect against acute gastritis and gastric injury and removes excess gastric acid. Black birch mushroom refers to a mushroom that grows by growing on birch trees. Black birch mushroom is a mushroom that grows in climatically growing birch trees in the north Korea, mainly 45 degrees north latitude, and is found in Russia, Finland, Canada, etc. have.

The anticancer and innate immune deficiency of black birch mushrooms are well known for their effectiveness, but only recently have scientific objectives proved their efficacy. However, the black birch mushroom has not yet been found to function as a therapeutic agent for gastritis and gastric ulcer. However, as a result of our research, the extract of the black birch mushroom acts on the gastrointestinal mucosa, which is significant for wound healing due to gastrointestinal inflammation or ulcer. It turned out to be effective. In addition, it was found that the extract of black birch mushroom showed gastric acid defensive function by combining with gastric acid and affecting gastric mucosa blood flow and prostaglandin which regulates acid secretion. In other words, the extract of the black birch mushroom can protect the gastric acid, a gastric ulcer attack factor, and at the same time exhibit a two-sided therapeutic effect of activating the protective factor, prostaglandin. Therefore, in the present invention, the hot water extract of the black birch mushroom has proved to have sufficient use as a therapeutic material effective for acute gastritis and gastrointestinal damage and also has a very low toxicity.

Accordingly, the present invention provides an anti-inflammatory composition comprising an extract of black birch mushroom in an amount sufficient to treat inflammation, and a method for treating inflammation by administering the same. The composition of the present invention may comprise a pharmaceutically acceptable carrier. Carriers include solvents, dispersion media, absorption retardants, and the like commonly used in the pharmaceutical art. The pharmaceutical composition of the present invention may be administered via any general route as long as it can reach the desired tissue. Thus, the compositions of the present invention can be administered topically, orally, parenterally, intranasally, intravenously, intramuscularly, subcutaneously, intraocularly, transdermally, and formulated into solutions, suspensions, tablets, pills, capsules, sustained release formulations, and the like. can do. Preferred formulations are injections. Dosage is determined in consideration of the skill of the art depending on the type and extent of the disease, age, sex, etc. of the patient.

Furthermore, the present invention provides an anti-cancer to anticancer composition comprising an effective amount of black birch mushroom extract and a method for preventing cancer by administering the same. The pharmaceutical composition of the present invention may comprise a pharmaceutically acceptable carrier. Carriers include solvents, dispersion media, absorption retardants, and the like commonly used in the pharmaceutical art. The pharmaceutical composition of the present invention may be administered via any general route as long as it can reach the desired tissue. Thus, the compositions of the present invention can be administered topically, orally, parenterally, intranasally, intravenously, intramuscularly, subcutaneously, intraocularly, transdermally, and formulated into solutions, suspensions, tablets, pills, capsules, sustained release formulations, and the like. can do. Preferred formulations are injections. Dosage is determined in consideration of the skill of the art depending on the type and extent of the disease, age, sex, etc. of the patient.

The present invention provides a cancer prevention or anti-inflammatory food composition containing the extract of the black birch mushroom. The food used as a base material is not specifically limited, Various foods, such as drinks, confectionery, bread, noodles, seasonings, such as water, a soft drink, or fruit drink, can be based on. The food of this invention is easily made by adding the process of adding the composition of this invention mentioned above to the manufacturing process of the base food, or by adding the process of adding the composition of this invention mentioned above after manufacture of the food of base materials. You can get At this time, you may add a taste and odor correction agent as needed. The content of the above-mentioned composition in the food of the present invention varies depending on the type and preference of the intended food, but the black birch mushroom may be used to fully utilize the preventive and health treatment effects of the black birch mushroom. The plant extract containing the extract is preferably at least 0.1% by weight. Thus, the food of this invention obtained is a food which can fully utilize the health prevention effect and health treatment effect which the black birch mushroom extract originally had because it contains the composition of this invention.

Hot water extract of black birch mushroom is preferably administered orally three times a day within the range of 100mg to 5,000mg. The dosage can be adjusted according to the degree of gastritis and ulcer and / or age of the patient.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are illustrative of the present invention, and the content of the present invention is not limited by the examples.

Example 1

Preparation of Samples of Black Birch Mushrooms and Preparation of Hot Water Extracts

Black birch mushrooms were collected and used near Lake Baikal in Irkutsk Autonomous Region, Siberia, Russia. The black birch mushroom used in the experiment was dried in the shade until there was no change in weight at room temperature, and then the dried sample was pulverized finely to be used as an extraction material.

The dry pulverized black birch mushroom (dry weight, 3.0 kg) was put into a 5 liter round flask, and 3.0L of distilled water was added thereto, followed by treatment with water in a hot water at 60 ° C. for 48 hours to extract the active ingredient. The distilled water extract was filtered using filter paper (Watman 1 time, Whatman), and then the filtrate was taken and extracted twice with distilled water in the same manner. The filtrate obtained through the above process was freeze-dried and concentrated. As a result, 67 g of distilled water extract was obtained.

Example 2

Acute Toxicity Test

To examine the toxicity of the hydrothermal extract of black birch mushroom, a large amount of samples were orally administered at a time to visually observe the toxicity in experimental animals. Ten male rats (breed: ICR) weighing 20 to 30 g were treated as group 1 with oral administration of hot water extract of black birch mushrooms at 5,000 mg / kg body weight, and observed abnormal behavior. Acute toxicity was observed by measuring. The results of the acute toxicity test up to 72 hours after the hydrothermal extract administration of black birch mushroom are shown in Table 1 below. In other words, there were no deaths and the minimum lethal dose of hot water extract of black birch mushroom was over 5,000mg / kg, and no behavioral abnormalities were observed during the observation period.

Table 1. Acute Toxicity Test Results of Hot Water Extract of Black Birch Mushrooms

Example 3

Hydrochloric acid-ethanol gastric damage test

In order to confirm the increased secretion of mucus or the presence of a defensive factor to the acid, an experiment was conducted using the Okabe method that causes damage to the stomach wall by direct administration of free hydrochloric acid, an attack factor.

Six Sprague-Dawley male rats, weighing 150-180 g, were fed as a group and fasted for 24 hours or more with water freely supplied, followed by oral administration of the sample, and 30 minutes later with hydrochloric acid-ethanol solution (60 1 ml of 150 mM HCl) was orally administered to% ethanol. After leaving for 1 hour under water-saving, the stomach was removed by lethality with ether, the stomach was fixed with 2% formalin solution, and after 5 minutes, the Taiwanese section was cut out to sum up the damage area.

For the measurement of the experimental results, the area of gastric ulceration was measured by measuring the width and length in millimeters under the low magnification microscope (10 times magnification), and the total area of the stomach was summed up and the treatment rate was calculated as follows.

Treatment Rate (%) = [(Control Ulcer Area-Drug Ulcer Area) / Control Ulcer Area] * 100

As a control drug, scrawlate (sucralfate), which is used as a coating agent (gastric wall protecting agent), was used. This experiment induced gastric damage by the administration of free hydrochloric acid, which was a protective effect of hot birch extract of black birch, suggesting increased mucus secretion or the presence of a defense factor against HCl. Could.

Table 2. Protective effect against hydrochloric acid-ethanol gastric injury

Table 2 shows the gastric protective effect of the hydrothermal extract of black birch mushroom at each concentration. As shown in Table 2, the gastric protective effect of the hydrothermal extract of the black birch mushroom was clear. The hot water extract of black birch mushroom showed a significant effect in the group administered more than 1,000mg / kg, showing a similar treatment effect to sucralate, an ulcer drug, and the protective effect of the hot water extract of black birch mushroom was very good. Could. In particular, the hydrothermal extract of black birch mushroom showed a significant effect even at low concentration (100mg / kg administration).

Example 4

Indomethacin Gastric Injury Experiment

This experiment was an experimental model by Yamagaki et al. That an experimental model of gastric ulceration was induced by suppressing the synthesis of prostaglandin, a gastric wall protective factor, when an excessive amount of nonsteroidal anti-inflammatory drugs were administered (Yamakaki K, et al. 1989, JAP J). Pharmacol., 49: 44.1-448.) Therefore, the gastric protective effect in this experiment affects the action of prostaglandin and the protective effect of gastric wall (Robert, A., Zamis, JENE). , Lan caster, C. and Hancher, AF: Cytoprotection by prostaglandins in Rats.Gastroenterology, 77,433-443 (1979))

Six SD male rats weighing 200-250 g were used as a group and fasted for 24 to 48 hours while freely supplying water. The samples were orally administered and subcutaneously injected with 25 mg / kg of indomethacin (indomethacin) 30 minutes later. After 7 hours of subcutaneous injection, the stomach was removed by ether, followed by fixation of the stomach with 2% formalin solution. Treatment rate was measured in the same manner as the hydrochloric acid-ethanol gastric injury experiment.

Cimetidine was used as a control drug. Nonsteroidal analgesic agents, such as indomethacin and aspirin, cause gastric ulcers by inhibiting the synthesis of prostaglandins in large doses. Therefore, it can be seen that the hot water extract of black birch, which showed a gastric protective effect in this experiment, affected the action of prostaglandins.

Table 3. Protective effect against indomethacin gastric injury

Table 3 shows the gastric protective effect of the hydrothermal extract of black birch mushrooms at each concentration. As can be seen in Table 3, it was found that the gastric protective effect of the hydrothermal extract of black birch was clear. The hydrothermal extract of black birch mushroom showed a significant effect in the group administered more than 1,000mg / kg, showing a similar therapeutic effect to cimetidine, an acid secretion inhibitor, and thus, it was confirmed that the hot water extract of black birch mushroom was excellent in protecting the stomach wall. In particular, the hot water extract of black birch mushroom showed a significant effect even at low concentrations (10 mg / kg administration).

Example 5

Acetic acid ulcer test

Acetic acid ulcer experiments are designed to measure the effectiveness of treatment for chronic ulcers and to indirectly measure the effects of prostaglandins on ulcers (Takagi K, et al, 1969, Jap J Pharmacol 199: 418). -426.)

Six SD male rats weighing 200-250 g were grouped as one group, and the stomach was exposed under ether anesthesia and exposed to 0.05 ml of 10% acetic acid at the grandular of the anterior wall. Injected into a sub-serosal layer using a micro-syringe and then sutured. After that, the animals were fed with normal feeding and watering, and the samples were orally administered once a day at a predetermined time for 10 days from the second day after the operation. On the 12th day after surgery, the stomach was removed by ether, and the stomach was fixed with 2% formalin solution for 5 minutes. Treatment rate was measured in the same manner as the hydrochloric acid-ethanol gastric injury experiment. Thus, as shown in Table 4, hot water extract of black birch mushroom showed a significant treatment rate in chronic ulcer caused by acetic acid.

Table 4. Healing Effect on Acetic Acid Ulcer

Reports of natural therapeutic effects and prostaglandin production on acetic acid ulcers (Uchida, M., Kawano, O., Misaki, N. and Irino, O .: Healing process of acetic acid-induced gastric ulcer and gastric mucosal prostaglandin E generation level in rats.Jap. J. Pharmacol., 50, 366 (1989).), The therapeutic effect of acetic ulcers was said to be due to an increase in prostaglandin-E2. Therefore, hydrothermal extract of black birch mushroom was thought to be involved in prostaglandin production.

According to the experiment, the hot water extract of the black birch mushroom has very low toxicity, has an excellent gastric protective effect against gastric acid, affects the production of prostaglandin, a protective protective factor of the stomach, and promotes rapid recovery of chronic ulcers. As a treatment for gastritis and ulcers, there was no inferiority.

The hydrothermal extract extracted from the black birch mushroom according to the present invention can be applied to the purpose of treating and preventing gastric ulcer in humans and animals. In particular, it can be useful to protect the gastric mucosa from acute gastritis and gastric injuries, to protect the gastric injuries from excessive secreted gastric acid, and to help restore the cell membrane of damaged gastrointestinal mucosa.

The pharmaceutical composition of the present invention can be administered orally, for example, in tablets, capsules, granules, granules. Such formulations may be prepared by the corresponding pharmaceutical techniques using starch as a excipient, lower substituted hydroxypropyl cellulose, hydroxypropyl cellulose or carboxymethyl cellulose as binders, magnesium stearate or starch as lubricants, colorants as additives, flavours and fragrances as additives. It is possible to manufacture.

In addition, the hot water extract of the black birch mushroom according to the present invention can be used in food, beverages, tea and other dietary supplements.

Hot water extract of black birch mushroom is preferably administered orally three times a day within the range of 100mg to 5,000mg. The dosage can be adjusted according to the degree of gastritis and ulcer and / or age of the patient.

Claims (2)

A pharmaceutical composition for the treatment of acute gastritis and peptic ulcer, containing the hydrothermal extract of black birch mushroom as an active ingredient. The pharmaceutical composition for treating acute gastritis and peptic ulcer according to claim 1, wherein the hot water extract of the black birch mushroom is administered orally three times a day within the range of 100 mg to 5,000 mg.