KR20030079237A - Composition of anti-diabetes patient food using silkworm extract - Google Patents

Abstract

PURPOSE: Provided is a composition of anti-diabetic diet using a silkworm extract which has, as a natural product, more excellent bioactive functionality than insulin therapy. Therefore, it is useful for the prevention of diabetes. CONSTITUTION: A composition of anti-diabetic diet is manufactured by the steps of: freeze-drying a silk worm and extracting it with methanol; concentrating the extract under reduced pressure and freeze-drying the concentrate; adding 0.001-2.50 parts by weight of extracts of ginger, licorice root and cinnamon to 0.01-15.0 parts by weight of the concentrate and followed by addition of 0.001-1.0 parts by weight of citric acid, 0.001-1.0 parts by weight of vitamin C and 0.01-10.0 parts by weight of taurine; and packing the mixture into a 200ml can.

Description

Composition of anti-diabetic patient using silkworm extract {COMPOSITION OF ANTI-DIABETES PATIENT FOOD USING SILKWORM EXTRACT}

The present invention relates to an antidiabetic patient composition using silkworm extract.

According to the WHO report (1985), diabetes is classified into insulin dependent diabetes (type I), insulin independent diabetes (type II), and malnutrition diabetes (MRDM). Fortunately, 84% of diabetic patients in Korea are type II diabetes, independent of insulin (Lee et al., 1984). In particular, type II diabetes is more common among adults in their 40s and older in 1) obese and 2) less active, 3) complications of hypertension, 4) hepatic dysfunction, and 5) high levels of triglycerides in the blood. It has the characteristic to

In view of the above results, the five diabetes-induced characteristics are the same as those of today's chronic degenerative disease.

In addition, since 84% of Korean diabetics are non-insulin-type diabetes, it is necessary to develop functional foods having a preventive or protective effect of diabetes among natural products having higher physiological activity than insulin therapy.

The purpose of the present invention is to use functional antidiabetic foods having a physiologically active effect that can prevent the onset of diabetes and prevent complications by using silkworm powder extract (SWE) extracted from silkworm powder, which has been used as a folk remedy since ancient times. I'm trying to provide.

Until now, the study on the anti-diabetic effect of silkworms mainly relates to the anti-diabetic effect of silkworm powder which is commercially available by directly or in a capsule.

The present invention focuses on the fact that 84% of the diabetic patients in Korea are insulin-independent (type II) diabetes. Therefore, it is necessary to develop a functional food having a prevention or defense effect of diabetes among natural products having higher physiological activity than insulin therapy. We confirmed the fact and developed a functional antidiabetic patient with antidiabetic effect.

That is, the 5-year-old 3-day silkworm, which was studied to be the most physiological effect, was freeze-dried to be powdered in consideration of the destruction of the ingredients, and then extracted with methanol, concentrated under reduced pressure, and frozen and dried again to give no silkworm destruction. A functional antidiabetic patient was prepared by using silkworm powder extract having a complete component of and adding a odorant, a flavoring and palatability agent, vitamins, and anti-fatigue agents. The functional antidiabetic diet of the present invention is effective for insulin-independent patients and also for the health of adult patients who develop due to lack of exercise.

The silkworms of 5 days and 3 days were lyophilized, extracted with methanol and concentrated under reduced pressure, and then lyophilized extracts containing 0.01 ~ 15.0 parts of silkworm extract (SWE) completely stored as ginger, licorice, Cinnamon extract is added to each other to add 0.001 to 2.50 parts by weight of the odor, and the taste and taste enhancer are added to 0.001 to 1.0 parts by weight of citric acid and 0.001 to 1.0 parts by weight of vitamin C, and 0.01 to 10.0 parts by weight of taurine as an anti-fatigue. A total of 200 ml of the added and mixed composition cans are packaged and the functional antidiabetic food is shipped.

Diabetic test animals were injected into the tail vein so that streptozotocin 60 mg / kg BW was administered orally daily to Crj / Bgi rats suffering from diabetes-causing diabetes for 4 days to evaluate the anti-diabetic effect for 12 days. It was. The experimental materials, test methods, and experimental results of animal testing on the development of the antidiabetic patient diet are as follows.

1. Animal testing and feed composition

(1) Animals and experiment

Crj / Bgi rats (male, 200 ± 10 g) purchased from Biogenomics (Seoul) were preliminarily bred for two weeks in an animal feeding room, and then blood sugar content was 3 days after inducing diabetes with streptozotocin (STZ). Only rats of 300 mg / dl or more were used for the experiment.

In the in vivo experiment, 7 animals were divided into 4 groups, and the control group was only Greenbia diabetes mellitus (DM) without silkworm powder extract, and the silkworm powder extract (SWE) was added to Greenbia diabetes mellitus (DM) in the experimental group. The antidiabetic effect of 200 ml prepared with the addition of 300, 500 mg was evaluated by free feeding of antidiabetic patients from day 3.

The animal breeding room is automatically controlled with a 12-hour cycle (06: 00-18: 00) under constant temperature and humidity (22 ± 2 ℃, 65 ± 2% RH).

(2) Composition of experimental antidiabetic patient food

The feed composition used in this experiment was mixed with 33 g maltodextrin as carbohydrate, 4.44 g sunflower oil as lipid, 10 g sodium caseinate as protein and 10 g soybean protein. Vitamins A (77.78 μgRE), D ₃ (0.56 μg), E (1.12 mgα-TE), C (6.12 mg), Folic acid (27.78 μg), Niacin (1.89 mg NE), Biotin (7.78 μg) As minerals Ca (77.78 mg), P (77.78 mg), Fe (1.34 mg), Mg (38.89 mg), K (128 mg), Na (138 mg) were added. 200 ml cans of this were prepared so that the total energy amount was 200 kcal to form an antidiabetic patient.

2. Experimental materials

(1) Ingredients for antidiabetic patients

The silkworms at 5 weeks of age and 3 weeks of age were frozen and dried, extracted as methanol, filtered, frozen and dried by low-temperature centrifugation at 2,000 × g for 10 minutes, and anti-diabetic patients were formed.

(2) Reagent

As the reagents used in this experiment, streptozotocin (STZ) for experimentally causing diabetes, a kit reagent for measuring glucose (glucose), a blood glucose in blood, and other reagents for analysis were used as a special reagent made by Sisma. .

3. Experimental method

(1) Induction of diabetes and administration of patient type

Streptozotocin (STZ), which is widely used as a diabetes inducer, was dissolved in 0.1M sodium citrate buffer (pH 4.3) in a Crj / Bgi rat to 60 mg / kg BW and injected through the tail vein. Daily blood glucose levels were analyzed in the tail vein of diabetes-induced rats and were used in animal experiments using only rats with blood glucose levels of 300 mg / dl or more on the third day.

In the in vivo experiment, 7 animals were divided into 4 groups, and the control group included only Greenbia diabetes mellitus (DM) without the silkworm powder extract, and the silkworm powder extract (SWE) 100, 300, The anti-diabetic effect of 200 ml of 500 mg added and prepared free anti-diabetic patients was evaluated from day 3. However, since rats consume about 20 to 25 ml of liquid per day, the daily intake of silkworm powder extract in antidiabetic children corresponds to 15, 45 and 75 mg, respectively, even at 30 ml per day.

(2) measurement of blood glucose

Glucose content in serum isolated from blood collected from tail vein was measured by kit reagent (Sigma, Co., USA) by enzyme method. First, 5 μl of glucose standard solution (400 mg / dl) is added as a serum and a standard solution, respectively, and 1.0 ml of a coloring reagent is added thereto, followed by well mixing and reaction at 37 ° C. for 15 minutes. Absorption was measured at 505 nm with the blank containing the coloring reagent in distilled water, and the content of glucose was determined according to the following equation.

Glucose content (mg / dl serum) = (OD _sample / OD _{standard solution} ) × 400 ^*

^* Concentration of glucose standard solution

(3) Measurement of lipid component and AI

One of the causes of insulin independent type II diabetes is the accumulation of lipid components. Therefore, triglyceride (TG) content as a neutral lipid in serum was measured by kit reagent (Sigma Co., USA). Total cholesterol content in serum was measured by o -phthalaldehyde method according to Rudel et al. (1973) method to quantify total cholesterol content in serum based on standard calibration curve. In addition, the contents of low density lipoprotein (LDL) and high density lipoprotein (HDL) -cholesterol in serum were measured according to the method of Noma et al. (1978). The atherosclerotic index (AI), which is used as an indicator of atherosclerosis, known as an early symptom of adult disease, was calculated according to the following equation according to Haglund et al. (1991).

AI = [(Total cholesterol-HDL cholesterol)] / HDL cholesterol

4. Evaluation of Experimental Results

(1) Hypoglycemic Effects of Antidiabetic Patients

After preliminary breeding for two weeks, the blood glucose level was almost 86.8 to 87.6 mg / dl serum. To induce hyperglycemia, 5 mg of Crj / Bgi rats with blood glucose above 300 mg / dl serum were divided into 4 groups of 1 group and treated with 60 mg / kg BW of streppozotocin 100, 300 and 500, respectively. The blood glucose level was measured for 8 days with the 200 ml antidiabetic patient developed by adding and mixing to the mg-based Greenbia DM in a water bottle. The results are shown in Table 1.

As shown in Table 1, the blood glucose content at the 8th day of administration of the anti-diabetic SWE-100, SWE-300 and SWE-500 administration groups was 293.1 ± 32.3, 276.5 ± 32.4 and 262.2 ± 31.6 mg / dl serum, respectively. The blood glucose content (100%) was 84.6%, 80.5%, and 75.9%, respectively, and very significant hypoglycemic effect of 15.2%, 19.5%, and 24.1%, respectively, was observed.

^a mean (mg / dl serum) ± SD; ^b STZ-induced diabetes resulting in a blood glucose level of 300 or more rats (5 mice each); ^c compared to day 3

(%); SWE-100, 300, 600 dose group: daily silkworm powder extract 15, 45, and 75 mg intake; Significance test:

^* P <0.05 compared to the control group; ^** p <0.01; ^*** p <0.001.

The anti-diabetic children developed with this drug have no side effects and are expected to improve diabetes more effectively. In particular, if the diabetes is not severe, it is expected that the product of the present invention can be used to treat diabetes.

(2) Inhibitory effect of lipid component and arteriosclerosis index (AI)

Obesity and accumulation of triglycerides are the causes of the development of insulin-independent type II. Therefore, as a result of administering the blood glucose 300 mg / dl or more rats in the same manner as in (1), compared to the effects on the neutral lipid, cholesterol content and atherosclerosis (atherogenic index) is shown in Table 1.

The content of triglyceride (TG) in the SWE-100, SWE-300 and SWE-500 administration groups was 222.8 ± 27.6, 178.7 ± 24.9 and 135.9 ± 10.8 mg / dl serum, respectively, and the TG content of the control group (265.6 ± 21.1 mg / dl). seum: 100%), 16.1%, 32.7% and 48.8%, respectively, showed a significant inhibitory effect of TG. The total cholesterol content of the antidiabetic group was also 10.2%, 13.3%, and 26.3%, respectively, compared with the control group. In addition, the LDL-cholesterol content of the anti-diabetic group was 11.2%, 18.3%, and 24.8%, respectively, compared with the control group, and the significant inhibitory effect of total cholesterol was recognized.

^a mean ± SD; ^a % relative to the control; SWE-100, 300, 500 Administered group: Daily silkworm powder extract 15, 45, 75

mg intake; Arteriosclerosis index (AI) = [(total cholesterol-HDL-cholesterol)] / HDL-cholesterol; Significance test: ^* p <0.05 compared to control group; ^** p <0.01; ^*** p <0.001.

On the other hand, comparing the effects of these antidiabetic patients on the amount of HDL-cholesterol, which is called cholesterol suppressor, was significantly increased by 9.0% and 26.9% in the SWE-300 and SWE-500 administration groups, respectively. The effect was recognized.

In comparison of the total cholesterol content and HDL-cholesterol content, the atherosclerosis index (AI), which is known as an indicator of adult disease, showed that the AI of SWE-300 and SWE-500 groups was 5.87 ± 2.4 and 4.02 ± 3.21, respectively, 23.2% and 47.4% compared to the control group (7.64 ± 0.54: 100%) to reduce the AI very effectively, it can be seen that very effectively prevent the complications of diabetes.

Example 1

The silkworms of 5 days and 3 days were lyophilized, extracted with methanol and concentrated under reduced pressure, and then, again, the extracts of ginger, licorice and cinnamon were used as a odor remover in 0.5 parts by weight of silkworm extract (SWE) in which the physiologically active ingredients were completely preserved 0.02 parts by weight is added to purify the odor, 0.05 parts by weight of citric acid and 0.05 parts by weight of vitamin C are added as a food and taste enhancer, 0.1 parts by weight of taurine as an anti-fatigue agent is added and mixed, followed by 200 ml of can packaging. Functional antidiabetic patients were prepared, and compared with the test results of the prepared by weight parts of each component shown in the following table, the weight parts were the most effective.

In the present invention, the beverage of the present invention is very effective in insulin-independent (type II) diabetics, which is the most difficult to treat among adult diseases caused by excessive nutrition of triglycerides and lack of exercise due to nutritional preference. Was confirmed by the animal experiment.

Therefore, when taking the anti-diabetic patient of the present invention, the hypoglycemic effect was significantly recognized.

Claims (1)

The silkworms of 5 days and 3 days were lyophilized, extracted with methanol and concentrated under reduced pressure, and then lyophilized extracts containing 0.01 ~ 15.0 parts of silkworm extract (SWE) completely stored as ginger, licorice, Add 0.001-2.50 parts by weight of cinnamon extract to purify the odor, add 0.001-1.0 parts by weight of citric acid and 0.001-1.0 parts by weight of vitamin C, and add 0.01-10.0 parts by weight of taurine as an anti-fatigue agent. A functional antidiabetic patient's composition prepared by mixing and then preparing a total 200 ml can packaging.