KR20030041738A - Cosmetic composition containing lysine added succinate or sulfonated atelocollagen and hyaluronic acid as essential ingredients - Google Patents

Abstract

PURPOSE: A cosmetic composition containing, as an essential component, anionized atelocollagen, hyaluronic acid and lysine which have excellent skin moisturizing, anti-aging and wrinkle inhibiting properties is provided. Therefore, the composition improves skin elasticity, provides skin softness and improves skin moisturization. CONSTITUTION: A cosmetic composition contains succinated or sulfonated atelocollagen, hyaluronic acid and lysine as an essential component. The composition is applied to essence, cream, lotion or pack. For an example, 0.1g of freeze dried succinated atelocollogen is dissolved in 10ml of purified water to produce a succinated atelocollogen solution. And 0.1g of hyaluronic acid and 0.05mg of lysine are dissolved in 10ml of purified water to produce a hyaluronic acid solution. The succinated atelocollogen solution and hyaluronic acid solution are mixed with preservatives, additives and flavors.

Description

Cosmetic composition containing lysine-added succinate or sulfonated atelocollagen and hyaluronic acid as essential ingredients

The present invention relates to a cosmetic product comprising a combination of succinate or sulfonated atelocollagen and hyaluronic acid having excellent performance in skin moisturizing, anti-aging, and anti-wrinkle, and more particularly anionized collagen, which is a component of skin tissue. The present invention provides a cosmetic composition containing lysine as a composition for improving the adhesion between succinate or sulfonated atelolol and hyaluronic acid and their cell surface.

The skin is composed of epidermis, dermis and subcutaneous fat tissue. The epidermis is composed of keratinocytes, granules, stratum corneum and basal layer by keratinocytes, and the dermis is composed of fibrous cells. Epidermal and dermal cells are maintained anatomically by major proteins such as collagen and elastin and extracellular matrix including glycosaminoglycans.

Wrinkles occurring with increasing age are known to be caused by two natural phenomena. In other words, the dermis corresponding to the deeper skin is exposed to repetitive tension by muscle activity located in the deeper to form wrinkles of the skin according to the deformation of the anatomical structure. Wrinkles of the epidermis are of course directly controlled by the wrinkles generated in the dermis, but on the other hand, it is known to form a thin wrinkle layer with the aging of keratinocytes. That is, the keratinocytes continuously generated from the base layer for a lifetime continue to move to the surface of the skin and reach the stratum corneum through the polar layer and the granule layer, and when exposed to oxygen in the air, the keratinocytes are exfoliated and spontaneously detached from the epidermis. It is known that when the rate of change to the stratum corneum gradually increases with age, the harmony with the stratum corneum collapses and a light wrinkle is formed, and the wrinkle formation is accelerated when the tension caused by muscle movement is added thereto. This is because the proper amount of collagen in the extracellular matrix maintains the structure of tissue by maintaining the mechanism of synthesis and degradation, but the synthesis gradually decreases with age, and the hyaluronic acid that moisturizes also decreases. It is known to be directly related.

Therefore, a method of relieving skin wrinkles by supplementing collagen, a major component of extracellular matrix, to skin tissues has been introduced in various ways. In general, deep wrinkles formed from the dermis are used to subcutaneously inject collagen to elevate the skin and remove the wrinkles morphologically.However, collagen injected is absorbed over time, and its effect is lost and does not affect the wrinkles of the epidermis. can not do it. In general, collagen is known to be effective in reducing the fine wrinkles that appear on the skin because it plays an important role in maintaining the tension and flexibility of the skin because of its high binding force with water as well as the structure as an extracellular matrix in the epidermal layer. . The method of directly applying collagen has been introduced as a method of alleviating the wrinkles of the epidermal layer. However, since large collagen molecules having a molecular weight of more than 300,000 are difficult to penetrate the surface of the stratum corneum, various methods have been devised to penetrate the stratum corneum and granule layer.

For example, cosmetic raw materials (2000, Korean Patent Laid-Open Publication No. 2000-0029382) containing a dispersion of collagen association products obtained by adjusting an aqueous solution of collagen having a specific isoelectric point to a pH close to the isoelectric point, and the ends of collagen molecules. A process for producing atelocollagen by selectively removing the telopeptide present in the portion; and saturation and esterification of the atelocollagen to generate collagen with increased solubility in a predetermined pH region (especially in a neutral pH region); Or the resulting succinate collagen and esterified collagen are emulsified and mixed in a mixture mainly of fats and oils, alcohols, surfactants and water, or dissolved in a mixture of alcohols and water (surfactant if necessary) or in water. Cosmetic manufacturing method characterized in that there is a process to make (1980, Japanese Laid-Open Patent Publication: 昭55-28947), after modifying animal tissues containing collagen, such as esterification of carboxyl groups present in collagen molecules with alcohol or acylation of amino groups present in collagen molecules with hydrochloric acid or acid anhydride, followed by extraction of the modified product. And a method for producing modified collagen characterized in that the modified formula is esterification (1996, 1 minute Patent Publication: 特 공개 平: 8-27192) and the like. In addition, a method of preparing crosslinked collagen or atelocollagen with a glycidyl group as a cosmetic collagen capsule coating agent has been introduced (Korea Patent Registration: SPECIAL 0143385).

The contents of the above patents have the advantage of not only reducing the size but also causing an immune response in the human body by removing the telopeptides present at both ends of the collagen molecule having a size of about 300 nanometers in length and about 2.4 nanometers in diameter. The atelo collagen thus prepared is soluble in trace amounts of hydrochloric acid or acetic acid due to acid solubility, but is insoluble in purified water due to water insolubility (generally, the ingredients used in cosmetics can be formulated at pH 5-9). It can be used as a cosmetic raw material) Various actions such as chemical modification by esterification, chemical modification by succinization and chemical modification by sulfonation to increase solubility by changing electrical properties of collagen molecule through chemical formula Genitals are introduced and succinized collagen and sulfonated collagen In the case of introducing an anionic functional group such as such, it is proposed a method for obtaining a water-soluble atelocollagen that is dissolved at pH 5-9 suitable for use as a cosmetic raw material.

Hyaluronic acid, which is present in the extracellular matrix of skin tissue, is a type of glycosaminoglycan (GAG) .However, unlike conventional GAG, which is combined with protein, it exists as a form of proteoglycan. It can retain moisture and, upon absorption of moisture, forms a gel-like structure that can be easily filled with fibrous proteins. In addition, hyaluronic acid is known to play a role in absorbing and maintaining moisture in skin tissue, as well as in regulating pH, suppressing fine wrinkles and skin aging, and constituting the NMF (Natural Moisturizing Factor) of the stratum corneum. Lonic acid is reduced, weakening the skin's moisturizing function.

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1 is a picture of crystal biot staining after the toxicity test for cells of the cosmetic composition of the present invention,

Figure 2 is a photograph showing the results of the solubility test of the cosmetic composition,

3 is a photograph of the degree of penetration test

4 is a penetration test graph,

5 is a photograph after the wrinkle relaxation test.

The present invention provides a cosmetic composition containing succinate or sulfonated atelocollagen, hyaluronic acid and lysine as essential ingredients, which have excellent performance in moisturizing, anti-aging, and anti-wrinkle of skin, and in particular, lysine is a skin of these components. Improves adhesion.

Lysine is a basic amino acid and is an essential amino acid that is not synthesized in the body. Until now, when lysine is used as a cosmetic raw material, a cationic polylysine is mixed with an anionic amino acid in order to promote hydrolysis in a hair care cosmetic composition as a polyamino cationic agent (2001, (Sec. 2001-0012619) is a representative example, but cosmetic preparations using very strong cell membrane adhesion among lysine properties have not been suggested.

The present invention facilitates skin penetration of atelocollagen by adding lysine to the composition consisting of anionized atelocollagen and hyaluronic acid, and once penetrated collagen maintains high adhesion to the cell surface by lysine, For all cosmetics, such as essences, creams, lotions, emulsions, lotions, packs to maintain the moisturizing effect. In other words, applying anionized atelocollagen penetrates the intercellular spaces of the epidermis and penetrates the stratum corneum and some granule layers of the skin, filling the intercellular voids widened with cellular aging, thereby maintaining the tension between cells. Hyaluronic acid acts as an NMF of the skin, thereby providing a moisturizing effect. Lysine is absorbed into the stratum corneum of the skin and connects the cells of the stratum corneum by secondary bonds to further increase the effect of anionized atelo collagen. By such an effect, the cosmetics containing lysine, anionized atelocollagen, and hyaluronic acid presented in the present invention as a composition impart softness to the skin and exhibit a fine wrinkle removal effect.

As a description for this, the process for preparing succinized atelocollagen and sulfonated atelocollagen, which is a composition of the anionized atelo cosmetics, is shown below, and the anionized atelocollagen, hyaluronic acid and lysine prepared according to the present invention The example of the manufacturing method of all the cosmetics containing as an example is illustrated. However, this embodiment does not limit the scope of the present invention.

Example 1

Succinic Atelocollagen Manufacturing Process

Add 2 mL of 1N acetic acid solution to 100 ml of distilled water, add 0.1 g of atelocollagen, and dissolve completely while stirring at room temperature. When atelocollagen is completely dissolved in distilled water containing acetic acid solution, adjust pH to 10 using 4N sodium hydroxide solution, and add 0.1 g of succinic anhydride. At this time, since the pH of the atelocollagen and succinic anhydride reacts, the pH decreases, and the mixture is stirred at room temperature for 1 hour while maintaining the pH at 10 using 4N sodium hydroxide solution to complete the succilation reaction of atelocollagen. After completion of the reaction, the succinized atelocollagen solution was adjusted to pH 4 using 1N hydrochloric acid solution at 4 ° C. to produce a white precipitate, and the precipitate was separated by centrifugation at 1500 rpm for 20 minutes. 0.1 g of the separated precipitate was dissolved in 10 mL of distilled water, adjusted to pH 7 using 4N sodium hydroxide, dialyzed for 2 days using a dialysis membrane capable of dialysis of molecules having a molecular weight of 40000, and lyophilized by lyophilization. Obtain collagen.

Example 2

Sulfonated Atelocollagen Manufacturing Process

Add 2 mL of 1N acetic acid solution to 100 ml of distilled water, add 0.1 g of atelocollagen, and dissolve completely while stirring at room temperature. After complete dissolution of atelocollagen in distilled water containing acetic acid solution, adjust the pH to 10 using 4N sodium hydroxide solution, add 0.1g of taurine and maintain pH 10 using 1N sodium hydroxide solution at room temperature for 12 hours. Stir to sulfonate atelocollagen. The reaction completes the sulfonated atelocollagen solution at 4 ° C. with 1N hydrochloric acid solution to pH 4 to form a white precipitate, which is separated by centrifugation at 1500 rpm for 20 minutes. 0.1 g of the separated precipitate was dissolved in 10 mL of distilled water, adjusted to pH 7 using 1N sodium hydroxide, dialyzed for 2 days using a dialysis membrane capable of dialysis of a molecular weight of 40000, and freeze-dried sulfonated atherosclerosis. Obtain collagen.

Example 3

0.1 g of lyophilized succinate atelocollagen was added to 10 mL of purified water to dissolve at room temperature to prepare a succinized atelocollagen solution. A hyaluronic acid solution was prepared by dissolving 0.1 g of hyaluronic acid and 0.05 mg of lysine in 10 mL of purified water. After preparing the succinated collagen solution and hyaluronic acid solution to prepare a cosmetic composition by adding a preservative, adjuvant and flavoring.

Example 4

1 g of lyophilized succinate atelocollagen was added to 200 mL of purified water to dissolve at room temperature to prepare a succinate collagen solution. After dissolving 1 g of hyaluronic acid and 0.5 mg of lysine in the prepared succinate atelocollagen solution, a preservative, adjuvant, and flavoring agent are added to prepare a cosmetic composition.

Example 5

0.1 g of lyophilized sulfonated atelocollagen was added to 10 mL of purified water and dissolved at room temperature to prepare a sulfonated collagen solution. A hyaluronic acid solution was prepared by dissolving 0.1 g of hyaluronic acid and 0.05 mg of lysine in 10 ml of purified water. After preparing the sulfonated atelocollagen solution and hyaluronic acid solution to prepare a cosmetic composition by adding a preservative, adjuvant and flavoring.

Example 6

1 g of lyophilized sulfonated atelocollagen was added to 200 mL of purified water and dissolved at room temperature to prepare a sulfonated atelocollagen solution. After dissolving 1 g of hyaluronic acid and 0.5 mg of lysine in the prepared sulfonated atelocollagen solution, a preservative, adjuvant, and flavoring agent are added to prepare a cosmetic composition.

Next, the effect of this invention is illustrated as an experimental example.

Experimental Example 1

Toxicity Tests on Cells of Cosmetic Products Prepared by the Patent:

In the cytotoxicity test using the cosmetic composition of Example 3, L929 fibroblasts were cultured to a certain concentration, and then the cosmetics were tested for cytotoxicity by indirect extraction.

The results are shown in FIG.

As a result, the cosmetic composition of the present invention was confirmed that there is no toxicity as a result of cytotoxicity test.

Experimental Example 2

Solubility test results of the cosmetic composition of the present invention:

Calf skin originated type I collagen purchased from Sigma (St. Louis, MO, USA) was used as a standard substance, and the rest of the ingredients were used in the same cosmetics. Compared.

The results are shown in FIG.

As a result of the solubility test of the cosmetic composition of the present invention, it was confirmed that the solubility of the sample containing anionized collagen was much better.

Experimental Example 3

Penetration test results of the cosmetic composition of the present invention

As a standard material, a solution containing Calf Skin Original Type I collagen and a solution containing only hyaluronic acid purchased from Sigma (St. Louis, MO. USA) were used. After loading onto the scaffolds, the scaffolds were placed in an electric field to create an electrical environment similar to that of the skin to compare penetration.

The photograph of the post-testing degree of penetration is shown in FIG. 3, and the graph of penetration is shown in FIG. 4.

As a result, the penetration rate of the sample containing the anionized collagen which is the composition of the present invention was excellent.

Experimental Example 4

Skin test results of cosmetic composition of the invention

In order to observe the degree of relaxation of the wrinkles by the cosmetic composition prepared by the present invention, after applying the cosmetics to the fine wrinkles on the back of the hand once a day, the degree of relaxation of the wrinkles was observed after 1 day, 5 days, 10 days.

5 is a photograph after the wrinkle relaxation test.

As a result of the photograph of Figure 5, it can be confirmed that the fine wrinkles on the back of the hand of the cosmetic composition of the present invention is alleviated.

The cosmetic composition containing lysine, anionized atelo collagen and hyaluronic acid produced by the present invention as the main component is absorbed and filled into the stratum corneum and some granule layers through intercellular skin pores to maintain the tension of the skin and impart flexibility, It enhances the moisturizing effect and is excellent in suppressing wrinkle formation and fine wrinkles on the skin surface.

Claims (2)

A cosmetic composition containing lysine, anionized atelo collagen and hyaluronic acid as main components. The cosmetic composition of claim 1 wherein the cosmetic composition is selected from the group consisting of essences, creams, lotions, packs.