KR20030032678A - Method of culturing Phellinus linteus by seed fungus which incubated in culture material - Google Patents

Abstract

PURPOSE: A method of cultivating the titled product which solves the problem to removal of sawdust or the like during processing Phellinus linteus after cultivation of fruit bodies thereof is provided. Therefore, the product simultaneously contains a ginseng component having excellent anticancer activity and increasing immune function and a Phellinus linteus component exhibiting anticancer effect and thus provides an anticancer effect superior to conventional Phellinus linteus. CONSTITUTION: The ginseng Phellinus linteus is prepared by the following process: culturing stains of Phellinus linteus in a cereal medium to produce grain spawn; adding ginseng powder and ginseng extract to edible cereal to produce a ginseng medium which is filled into a culture vessel; sterilizing the culture vessel containing the ginseng medium; inoculating the grain spawn into the ginseng medium; and culturing the grain spawn for a specified period. The edible cereal is one of brown rice, polished rice, barley, wheat, corn, bean, adlay, sorghum and millet. The ginseng medium contains 3 to 10% by weight of ginseng.

Description

Method of culturing Phellinus linteus by seed fungus which incubated in culture material}

The present invention relates to a situation mushroom cultivation method, and more particularly to a ginseng situation mushroom cultivation method of cultivating ginseng situation mushroom excellent anti-cancer effect by making a culture medium with ginseng and edible grains.

In general, mushrooms are a generic term for a herd of fungi that form fruiting bodies of a size discernible to the eye. In other words, the fungus means a true fungus except bacteria and slime molds among lower plants that do not have chlorophyll. Most mushrooms belong to basidiomycetes with umbrella-shaped lampshades and bags. These mushrooms are composed of mycelium, a nutritive organ, and a fruiting body with spores, which are breeding organs. The fruiting layer is a layer of porcine vesicles or biliary disease layered side by side. Spores formed in the fruiting body germinate to become the first mycelia, and the compatible mycelium forms a cellular fusion with each other to become the secondary mycelia, and when it is ingested with sufficient nutrition, the mycelium is organized under the proper environment and forms the third fruit hyphae. Done. In addition, mushrooms can be classified into forest-producing fungi on the ground and wood-producing fungi sprouting from old trees.

Phellinus linteus is a perennial mushroom belonging to the order of Aphylloporales, Pine scales (Hymenochaltaclal), and mud mushrooms, and growing on old trees such as mulberry. . In other words, the situation mushroom is a woody mildew fungus is known to produce a polysaccharide-based anti-cancer immunoactive substance as a polysaccharide as a result of liquid culture of the mycelia obtained from the fruiting body as a test strain. This anti-cancer immuno-active substance is safe in terms of toxicity due to its low side effects, and it has an anti-cancer effect by enhancing the immune function in the human body in relation to T cells, B cells, NK cells, etc., and promoting the production of cytokines such as interleukin or interferon. It is being developed as an alternative medicine.

Artificial cultivation of such situation mushrooms are usually grown in the order of securing a strain, culturing mycelium from the strain in a culture medium, and then growing the situation mushroom in which fruiting bodies are formed from the mycelium in a cultivator.

Here, as a conventional method of cultivating situation mushrooms, cultivation methods using wood cultivation, sawdust cultivation, grains or liquid medium are known. For example, according to the registration number 10-226632, published October 15, 1999 in the Republic of Korea Patent Publication as a conventional situation mushroom cultivation method, culturing the strain using agar medium in the preparation of the seed, and the situation mushroom cultivation Culture medium for is composed of sawdust obtained from mulberry sawdust and bark, alder, oak, and aspen trees as a main ingredient, mixed in an appropriate ratio, and then added with a small amount of calcium acetate, sugar, rice bran and flour.

By the way, the medium using the sawdust of the wood, such as mulberry has a problem that processing is inconvenient to remove sawdust, etc. when processing the situation mushroom after fruiting body culture. And it would be very desirable from the perspective of national health if you can grow a situation mushroom more improved than the conventional situation mushroom.

The present invention aims to solve the above problems and to provide a functional situation mushroom cultivation method having an improved anticancer effect.

1 is a flow chart showing a ginseng situation mushroom cultivation method using a grain spawn according to the present invention,

Figure 2 is a view showing a container for culturing grain spawn in the present invention.

* Explanation of symbols for main parts of the drawings

201: plastic container 202: silistov marge

203: grain medium

The situation mushroom cultivation method of the present invention to achieve the above object, the grain seed culture step of culturing the strain in a grain medium to obtain grain seed; A ginseng medium preparation step of adding ginseng powder or ginseng extract to edible grains to form a ginseng medium, and filling the ginseng medium into a culture vessel; Sterilization step of sterilizing the culture vessel in which the ginseng medium is inserted at high temperature and high pressure; Grain spawn inoculation step of inoculating the grain spawn on the sterilized ginseng medium; And a ginseng situation mushroom culturing step of obtaining a ginseng situation mushroom by culturing the grain spawn seed inoculated into the culture vessel under a predetermined condition.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The present invention is a cultivation method of the pellius strain of Felius linteus, cultivated strains identified by the National Institute of Agricultural Science and Technology (Eummi51818-5) cultivated in the grain medium of tissues or spores of fruiting bodies and then added red ginseng or white ginseng powder or extract It is a technology to obtain ginseng situation mushrooms superior to the existing situation by inoculating in culture medium made of cereals. Using the ginseng situation mushroom of the present invention produced in this way, ginseng situation mushroom rice, concentrated red ginseng situation mushroom liquid, powder, tea, liquid tea, beverage, tablets, granules, candy, gum, confectionery, etc. Can contribute to promotion.

On the other hand, ginseng is an exceptional medicine that has been proven in herbal medicine for thousands of years. According to the ancient book, the ancient book, "Ginseng mainly protects the five intestines, stabilizes the mind, stops the alertness, brightens the eyes, makes the head wise and takes a long life. Extends. " In addition to other names and herbs, the benefits of ginseng are also concentrated. In modern times, prominent scholars from all over the world have physiology through active scientific research. biochemistry. Pharmacology. Pathologically, the efficacy of ginseng has been studied, and many clinical studies have been published. According to these findings, ginseng promotes brain activity, is effective in preventing and treating diabetes, controlling blood pressure, and treating atherosclerosis hyperlipidemia. In particular, ginseng increases the immune function of cancer patients to strengthen the body's resistance to inhibit cancer cell growth and metastasis to other organs, and has the effect of preventing recurrence after surgery. In addition, the results of Murata et al. Found that white ginseng concentrate containing large amounts of saponin was taken for cancer patients such as gastric cancer, pancreatic cancer, colon cancer, breast cancer for 3 to 6 months or longer, and clinical observation showed that 69.7% of patients Progress and development was suppressed, and a 2000 paper published by Professor Saiki of Toyama University and Professor Lee Byung-hoon of Wonkwang University showed that saponin, a major active ingredient, was metabolized by intestinal bacteria when taking white ginseng. Water is said to provide strong anticancer effects, including cancer cell metastasis suppression and carcinogenic inhibitory effects.

In general, ginseng is harvested in the field called 'Ginseng', dried ginseng is called 'white ginseng', dried ginseng at 80-90 ℃ for 10-20 minutes and then dried 'Taeguksam', Steamed and dried ginseng is called 'red ginseng'. These classifications are about 75% of the water ginseng, so according to the type of treatment to prevent the deterioration in distribution, and the treatment process, although a slight component change occurs in the efficacy as a ginseng is similar. Therefore, in the present invention, variously treated ginseng, that is, ginseng, white ginseng, taegeuk ginseng, red ginseng, etc. collectively referred to as ginseng, this ginseng is made by crushing powder or extract extracted with a bath.

1 is a flowchart illustrating a method of cultivating ginseng situation mushroom using grain seed according to the present invention, Figure 2 is a view showing a container for culturing grain seed in the present invention.

Referring to Figure 1, the ginseng situation mushroom cultivation method of the present invention is a grain seed culture step of producing a grain spawn by culturing strains (Eung 51818-5: Felinus linteus), which has been identified and identified from the Institute of Agricultural Science and Technology in a grain medium ( S1), and the ginseng medium preparation step (S2) to add a powder or extract of ginseng (red ginseng or white ginseng) to the culture medium material of edible grains (rice, brown rice, etc.) and filling the culture vessel (S2), and the ginseng Sterilization step (S3) for sterilizing the culture vessel filled with medium in a high-temperature high-pressure sterilization pot, and grain seeding inoculation step (S4) for inoculating grain spawn in a sterilized ginseng medium in a clean room; Ginseng situation mushroom culture step (S5) of cultivating the seed inoculated in ginseng medium for 20 to 30 days at 25 ℃ and the cultured ginseng situation mushroom to process the ginseng situation mushroom Mushroom processing step (S6) is made.

1) Grain spawn culture step (S1)

Usually, strains of situation mushrooms are used to obtain strains from wild situation mushrooms or to receive strains from microbial deposit institutions or to purchase seed species from spawn culture. In the present invention, a strain cultured by the present inventors and identified as identified by the Institute of Agricultural Science and Technology (Eut. 51818-5: Felinus linteus) is used as a strain. Yeom 51818-5 is a genuine Felinus linteus, excellent anti-cancer effect, unlike conventional strains, and is officially recognized by the official institution. By culturing the excellent strain in the ginseng medium according to the present invention can obtain a ginseng situation mushroom excellent in functionality compared to other situation mushrooms.

The grain medium for culturing the spawn according to the present invention is contained in a plastic container 201, as shown in Figure 2, the grain medium 203 is a sense of sensitivity to edible grains such as rice, brown rice, barley, wheat, etc. A culture medium made by adding additives. After inoculating the seed medium into the grain medium 203, the inlet of the plastic container 201 is closed with a marguerite 202 made of silistope or the like and cultured for a predetermined period of time to obtain a grain strain. At this time, since the fine air holes are formed in the silistope, air necessary for culturing can be communicated.

2) mushroom culture medium preparation step (S2)

Culture medium for cultivating the situation mushroom according to the present invention is to add a variety of ginseng, such as ginseng, white ginseng, Taegeuk ginseng, red ginseng in the form of powder or extract to the material consisting of edible grains edible by humans. As such, the medium to which the ginseng component is added according to the present invention is simply referred to as 'ginseng medium'.

As the edible grains used in the present invention, brown rice, barley, wheat, corn, soybeans, barley, sorghum, crude, etc., which are widely known and used for food, may be used, and any one or two or more thereof may be selected and mixed. Can also be used.

And when making culture medium with edible grains, it is preferable to use the crushed mushroom mycelium so as to easily absorb the nutrients in the growing process, but it may be used as it is or may be used after being boiled or processed.

In addition, when the culture medium is made from ginseng and edible grains such as red ginseng or white ginseng, the higher the content of ginseng, the better the efficacy of the produced situation mushrooms, but considering the production cost, the weight ratio of ginseng extract or powder is about It is preferable that it is about 3 to 50% weight ratio. However, the weight ratio of the extract or powder of ginseng may be about 3 to 90% by weight, and in some cases, the culture medium may be made only with ginseng (ie, 100% by weight).

Red ginseng or white ginseng powder is made by grinding red ginseng or white ginseng, and the extract of red ginseng or white ginseng is made by boiling red ginseng or white ginseng. And ginseng can be used as it is after crushing the high moisture itself.

The red ginseng or white ginseng powder or extract thus prepared is mixed with edible grains to form a ginseng medium, and then the preparation of the culture medium is completed in a culture container (cultivation container) in the form of a heat resistant plastic bottle or a heat resistant film bag.

3) Sterilization step (S3)

When cultivated mushrooms, put the culture vessel filled with ginseng medium into a sterilization pot (heating vessel) so that various bacteria do not enter, and sterilize at a high temperature and high pressure of about 121 ℃. At this time, the culture vessel should be made of a material (for example, heat resistant plastic or heat resistant film) that can withstand high temperatures.

4) Grain spawn inoculation step (S4)

The sterilized culture vessel is transferred to a clean room and inoculated with the seed grain cultured in the seed seed culture step. At this time, the inoculation method inoculates the whole surface of the ginseng medium exposed to the air in the culture vessel so as to completely cover it with grain seed. This inoculation allows the mycelia inoculated in ginseng medium to absorb nutrients from previous grain medium while adapting to ginseng medium.

5) Ginseng situation mushroom culture step (S5)

After inoculation in a sterile chamber, the culture vessel is transferred to the mushroom cultivation room to incubate the ginseng situation mushroom. Mushroom culture is also incubated about 20-30 days while maintaining the temperature conditions about 25 ℃ ± 2 ℃.

Mycelia inoculated into ginseng medium extends to the inner surface of ginseng medium from the surface of ginseng medium by expanding new mycelia as the culture days elapse. Mycelia that glide on the surface of ginseng medium continue to secrete enzymes to break down the ginseng medium, which is then absorbed and used as energy for metabolism and growth. As described above, the ginseng medium is decomposed from the part where the mycelium of the spawn contacts, so that the ginseng medium penetrates into the interior as time passes, and eventually, the ginseng medium is degraded and absorbed. In particular, according to the present invention, because ginseng powder or ginseng extract is added to the ginseng medium, the main components contained in ginseng such as saponin are absorbed into the situation mushroom, and thus the anti-cancer effect is better than the existing situation mushroom It will be grown.

In this way, the cultivated situation mushrooms form a situation mushroom fruiting body whose yellow surface is in contact with the air, and the remaining grains and ginseng components remain at the bottom side.

6) Mushroom processing step (S6)

The ginseng situation mushrooms cultivated as described above are cut in a culture vessel, dried and processed in various ways. For example, the dried ginseng situation mushroom can be ground and powdered to make concentrated ginseng mushroom solution, and tea (liquid tea), beverages, tablets, granules, candy, gum, sweets, etc. can be prepared in various forms. Can be. It can also be mixed with rice to make ginseng situation mushroom rice.

As described above, the ginseng situation mushroom cultivated by the method of the present invention promotes anti-cancer effects by promoting the production of internationally recognized ginseng components and cytokines such as interleukin and interferon, which are excellent for anticancer activity and enhancing immune function in the human body. Containing the components of the present situation mushrooms at the same time provides a much better anti-cancer effect than the conventional situation mushrooms. And by processing and selling such excellent ginseng situation mushrooms in various forms of food can contribute to the improvement of national health, and can give new hope for treatment to various cancer patients known to be difficult to treat.

Claims (4)

Grain spawn culture step of obtaining a grain spawn by culturing the strain in a grain medium; A ginseng medium preparation step of adding ginseng powder or ginseng extract to edible grains to form a ginseng medium, and filling the ginseng medium into a culture vessel; Sterilization step of sterilizing the culture vessel in which the ginseng medium is inserted at high temperature and high pressure; Grain spawn inoculation step of inoculating the grain spawn on the sterilized ginseng medium; And Ginseng situation mushroom cultivation method using a grain seed culture, characterized in that consisting of the ginseng situation mushroom culture step of obtaining a ginseng situation mushroom by culturing the grain seed inoculated into the culture vessel under a predetermined condition. According to claim 1, The ginseng badge ginseng situation mushroom cultivation method using grain spawn, characterized in that the content ratio of 3 to 100% by weight. The method of claim 1, wherein the edible grains are brown rice, barley, wheat, corn, soybeans, yulmu, sorghum, crude ginseng situation mushroom cultivation method using grains, characterized in that any one. According to claim 1, The cultivation method, Ginseng situation mushroom cultivation method using grain spawn, characterized in that further comprising the step of collecting / drying the cultured ginseng situation mushroom.